Expression Analyses of Rich2/Arhgap44, a Rho Family GTPase-Activating Protein, during Mouse Brain Development
In this study, we carried out expression analyses of Rich2 by focusing on mouse brain development. In immunoblotting, Rich2 exhibited a tissue-dependent expression profile in the young adult mouse, and the expression was increased during brain development. In immunohistochemical analyses, Rich2 was observed in the cytoplasm of cortical neurons at postnatal day (P) 0 and then came to be enriched in the nucleus with moderate distribution in neuropils at P7. Later at P30, a complex immunostaining pattern of Rich2 was observed; Rich2 was distributed in the nucleus, cytoplasm, and neuropils in many cortical neurons, whereas oth...
Source: Developmental Neuroscience - January 11, 2023 Category: Neuroscience Source Type: research
Expression analyses of Rich2/Arhgap44, a Rho family GTPase-activating protein, during mouse brain development
In this study, we carried out expression analyses of Rich2 by focusing on mouse brain development. In immunoblotting, Rich2 exhibited a tissue-dependent expression profile in the young adult mouse, and the expression was increased during brain development. In immunohistochemical analyses, Rich2 was observed in the cytoplasm of cortical neurons at postnatal day (P) 0, and then came to be enriched in the nucleus with moderate distribution in neuropils at P7. Later at P30, complex immunostaining pattern of Rich2 was observed; Rich2 was distributed in the nucleus, cytoplasm and neuropils in many cortical neurons whereas other ...
Source: Developmental Neuroscience - January 11, 2023 Category: Neuroscience Source Type: research
Microglial Characterization in Transient Human Neurodevelopmental Structures
Human neurodevelopment is characterized by the appearance, development, and disappearance or transformation of various transient structures that underlie the establishment of connectivity within and between future cortical and subcortical areas. Examples of transient structures in the forebrain (among many others) include the subpial granular layer and the subplate zone. We have previously characterized the precise spatiotemporal dynamics of microglia in the human telencephalon. Here, we describe the diversity of microglial morphologies in the subpial granular layer and the subplate zone. Where possible, we couple the pred...
Source: Developmental Neuroscience - January 5, 2023 Category: Neuroscience Source Type: research
Microglial Characterization in Transient Human Neurodevelopmental Structures
Human neurodevelopment is characterized by the appearance, development, and disappearance or transformation of various transient structures that underlie the establishment of connectivity within and between future cortical and subcortical areas. Examples of transient structures in the forebrain (among many others) include the subpial granular layer and the subplate zone. We have previously characterized the precise spatiotemporal dynamics of microglia in the human telencephalon. Here, we describe the diversity of microglial morphologies in the subpial granular layer and the subplate zone. Where possible, we couple the pred...
Source: Developmental Neuroscience - January 5, 2023 Category: Neuroscience Source Type: research
Microglial characterisation in transient human neurodevelopmental structures
Human neurodevelopment is characterised by the appearance, development and disappearance or transformation of various transient structures that underlie the establishment of connectivity within and between future cortical and subcortical areas. Examples of transient structures in the forebrain (amongst many others) include the subpial granular layer and the subplate zone. We have previously characterised the precise spatiotemporal dynamics of microglia in the human telencephalon. Here, we describe the diversity of microglial morphologies in the subpial granular layer and the subplate zone. Where possible, we couple the pre...
Source: Developmental Neuroscience - January 5, 2023 Category: Neuroscience Source Type: research
Auditory Outcomes in Adolescents with Prenatal Alcohol Exposure
Investigate three aspects of auditory function (auditory acuity, cochlear dysfunction, and auditory processing) in adolescents with fetal alcohol exposure without phenotypic changes. Fifty-one adolescents with and without intrauterine exposure to alcohol were selected from a cohort study. The summons, evaluation, and analysis of the results were carried out blindly regarding the respective exposure to alcohol. The auditory tests were pure-tone audiometry, transient otoacoustic emissions, and behavioral assessment of auditory processing (speech-in-noise, dichotic digits, and gap-in-noise). After testing 45 adolescents were ...
Source: Developmental Neuroscience - December 29, 2022 Category: Neuroscience Source Type: research
Neurochemical Profile of < b > < i > BRAF < /i > < /b > < sup > V600E < /sup > / < b > < i > Akt < /i > < /b > < sup > T308D/S473D < /sup > Mouse Gangliogliomas Reveals Impaired GABAergic System Inhibition
In conclusion, changes in the levels of key proteins in neurotransmitter signaling suggest a loss of synapses and may thereby lead to ne uronal network alterations in mouse GGs. (Source: Developmental Neuroscience)
Source: Developmental Neuroscience - December 20, 2022 Category: Neuroscience Source Type: research
Neurochemical profile of BRAFV600E/AktT308D/S473D mouse gangliogliomas reveals impaired GABAergic system inhibition
In conclusion, changes in the levels of key proteins in neurotransmitter signaling suggest a loss of synapses and may thereby lead to n euronal network alterations in mouse GGs. (Source: Developmental Neuroscience)
Source: Developmental Neuroscience - December 20, 2022 Category: Neuroscience Source Type: research
Cross-sectional Observational Study of Typical in-utero Fetal Movements using Machine Learning
In conclusion, using ML we successfully quantified complex 3D fetal limb motion in-utero and across gestation, showing maternal factors (hyperoxia) and fetal factors (gestational age, joint) impact movement. Quantification of fetal motion on MRI is a potential new biomarker of fetal health and neuromuscular development. (Source: Developmental Neuroscience)
Source: Developmental Neuroscience - December 20, 2022 Category: Neuroscience Source Type: research
miR-506-3p relieves neuropathic pain following brachial plexus avulsion via mitigating microglial activation through targeting the CCL2-CCR2 axis
Neuroinflammation results in neuropathic pain following brachial plexus avulsion (BPA). This research was designed for investigating the function of miR-506-3p in BPA-induced neuropathic pain (NP). A total brachial plexus root avulsion (tBPRA) model was produced in adult rats as well as IL-1 β-treated motoneuron-like NSC-34 cells and the LPS-treated microglia cell line BV2 for in vivo and in vitro experiments, respectively. RT-PCR and western blot were performed to detect the profiles of miR-506-3p, CCL2 and CCR2, NF-κB, FOXO3a, TNF-α, IL-1β, and IL-6 in cells or the spinal cord clo se to the tBPI lesion. Neuronal apop...
Source: Developmental Neuroscience - December 5, 2022 Category: Neuroscience Source Type: research
Progesterone as a neuroprotective agent in neonatal hypoxic-ischaemic encephalopathy - a systematic review
Hypoxic-ischaemic encephalopathy (HIE) in the newborn baby is a major contributor to neonatal mortality and morbidity across the world. Therapeutic hypothermia (HT) is the current standard treatment for moderate to severe HIE, but not all babies benefit. Potential neuroprotective actions of PROG include anti-apoptotic, anti-inflammatory and anti-oxidative effects and reduction of energy depletion, tissue/cellular oedema and excitotoxicity. In pre-clinical studies of neonatal HIE, progesterone (PROG) has neuroprotective properties, but has not been the subject of systematic review. Here our objective was to evaluate the evi...
Source: Developmental Neuroscience - November 25, 2022 Category: Neuroscience Source Type: research
Association of Nogo-A gene polymorphisms with cerebral palsy in Southern China: a case-control study
This study showed that the SNPs rs1012603 of Nogo-A were significantly correlated with CP, and the correlations were also found in spastic diplegia, GMFCS I of CP, ADL (>9) of CP and female subgroups, indicating that Nogo-A might mainly affected mild types of CP and there might be sex-related di fferences. (Source: Developmental Neuroscience)
Source: Developmental Neuroscience - November 2, 2022 Category: Neuroscience Source Type: research
