Metaplastic regeneration in the mouse stomach requires a reactive oxygen species pathway
Dev Cell. 2024 Mar 19:S1534-5807(24)00142-4. doi: 10.1016/j.devcel.2024.03.002. Online ahead of print.ABSTRACTIn pyloric metaplasia, mature gastric chief cells reprogram via an evolutionarily conserved process termed paligenosis to re-enter the cell cycle and become spasmolytic polypeptide-expressing metaplasia (SPEM) cells. Here, we use single-cell RNA sequencing (scRNA-seq) following injury to the murine stomach to analyze mechanisms governing paligenosis at high resolution. Injury causes induced reactive oxygen species (ROS) with coordinated changes in mitochondrial activity and cellular metabolism, requiring the transc...
Source: Developmental Cell - March 23, 2024 Category: Cytology Authors: Zhi-Feng Miao Jing-Xu Sun Xuan-Zhang Huang Shi Bai Min-Jiao Pang Jia-Yi Li Han-Yu Chen Qi-Yue Tong Shi-Yu Ye Xin-Yu Wang Xiao-Hai Hu Jing-Ying Li Jin-Wei Zou Wen Xu Jun-Hao Yang Xi Lu Jason C Mills Zhen-Ning Wang Source Type: research
S-nitrosylation-triggered unfolded protein response maintains hematopoietic progenitors in Drosophila
Dev Cell. 2024 Mar 19:S1534-5807(24)00145-X. doi: 10.1016/j.devcel.2024.02.013. Online ahead of print.ABSTRACTThe Drosophila lymph gland houses blood progenitors that give rise to myeloid-like blood cells. Initially, blood progenitors proliferate, but later, they become quiescent to maintain multipotency before differentiation. Despite the identification of various factors involved in multipotency maintenance, the cellular mechanism controlling blood progenitor quiescence remains elusive. Here, we identify the expression of nitric oxide synthase in blood progenitors, generating nitric oxide for post-translational S-nitrosy...
Source: Developmental Cell - March 23, 2024 Category: Cytology Authors: Bumsik Cho Mingyu Shin Eunji Chang Seogho Son Incheol Shin Jiwon Shim Source Type: research
Metaplastic regeneration in the mouse stomach requires a reactive oxygen species pathway
Dev Cell. 2024 Mar 19:S1534-5807(24)00142-4. doi: 10.1016/j.devcel.2024.03.002. Online ahead of print.ABSTRACTIn pyloric metaplasia, mature gastric chief cells reprogram via an evolutionarily conserved process termed paligenosis to re-enter the cell cycle and become spasmolytic polypeptide-expressing metaplasia (SPEM) cells. Here, we use single-cell RNA sequencing (scRNA-seq) following injury to the murine stomach to analyze mechanisms governing paligenosis at high resolution. Injury causes induced reactive oxygen species (ROS) with coordinated changes in mitochondrial activity and cellular metabolism, requiring the transc...
Source: Developmental Cell - March 23, 2024 Category: Cytology Authors: Zhi-Feng Miao Jing-Xu Sun Xuan-Zhang Huang Shi Bai Min-Jiao Pang Jia-Yi Li Han-Yu Chen Qi-Yue Tong Shi-Yu Ye Xin-Yu Wang Xiao-Hai Hu Jing-Ying Li Jin-Wei Zou Wen Xu Jun-Hao Yang Xi Lu Jason C Mills Zhen-Ning Wang Source Type: research
S-nitrosylation-triggered unfolded protein response maintains hematopoietic progenitors in Drosophila
Dev Cell. 2024 Mar 19:S1534-5807(24)00145-X. doi: 10.1016/j.devcel.2024.02.013. Online ahead of print.ABSTRACTThe Drosophila lymph gland houses blood progenitors that give rise to myeloid-like blood cells. Initially, blood progenitors proliferate, but later, they become quiescent to maintain multipotency before differentiation. Despite the identification of various factors involved in multipotency maintenance, the cellular mechanism controlling blood progenitor quiescence remains elusive. Here, we identify the expression of nitric oxide synthase in blood progenitors, generating nitric oxide for post-translational S-nitrosy...
Source: Developmental Cell - March 23, 2024 Category: Cytology Authors: Bumsik Cho Mingyu Shin Eunji Chang Seogho Son Incheol Shin Jiwon Shim Source Type: research
Spatially resolved proteomics of the Arabidopsis stomatal lineage identifies polarity complexes for cell divisions and stomatal pores
Dev Cell. 2024 Mar 18:S1534-5807(24)00141-2. doi: 10.1016/j.devcel.2024.03.001. Online ahead of print.ABSTRACTCell polarity is used to guide asymmetric divisions and create morphologically diverse cells. We find that two oppositely oriented cortical polarity domains present during the asymmetric divisions in the Arabidopsis stomatal lineage are reconfigured into polar domains marking ventral (pore-forming) and outward-facing domains of maturing stomatal guard cells. Proteins that define these opposing polarity domains were used as baits in miniTurboID-based proximity labeling. Among differentially enriched proteins, we fin...
Source: Developmental Cell - March 22, 2024 Category: Cytology Authors: Eva-Sophie Wallner Andrea Mair Dominik Handler Claire McWhite Shou-Ling Xu Liam Dolan Dominique C Bergmann Source Type: research
Spatially resolved proteomics of the Arabidopsis stomatal lineage identifies polarity complexes for cell divisions and stomatal pores
Dev Cell. 2024 Mar 18:S1534-5807(24)00141-2. doi: 10.1016/j.devcel.2024.03.001. Online ahead of print.ABSTRACTCell polarity is used to guide asymmetric divisions and create morphologically diverse cells. We find that two oppositely oriented cortical polarity domains present during the asymmetric divisions in the Arabidopsis stomatal lineage are reconfigured into polar domains marking ventral (pore-forming) and outward-facing domains of maturing stomatal guard cells. Proteins that define these opposing polarity domains were used as baits in miniTurboID-based proximity labeling. Among differentially enriched proteins, we fin...
Source: Developmental Cell - March 22, 2024 Category: Cytology Authors: Eva-Sophie Wallner Andrea Mair Dominik Handler Claire McWhite Shou-Ling Xu Liam Dolan Dominique C Bergmann Source Type: research
Single-cell omics identifies inflammatory signaling as a trans-differentiation trigger in mouse embryos
Dev Cell. 2024 Mar 18:S1534-5807(24)00109-6. doi: 10.1016/j.devcel.2024.02.010. Online ahead of print.ABSTRACTTrans-differentiation represents a direct lineage conversion; however, insufficient characterization of this process hinders its potential applications. Here, to explore a potential universal principal for trans-differentiation, we performed single-cell transcriptomic analysis of endothelial-to-hematopoietic transition (EHT), endothelial-to-mesenchymal transition, and epithelial-to-mesenchymal transition in mouse embryos. We applied three scoring indexes of entropies, cell-type signature transcription factor expres...
Source: Developmental Cell - March 20, 2024 Category: Cytology Authors: Yifan Zhang Zhixin Kang Mengyao Liu Lu Wang Feng Liu Source Type: research
Synchronized assembly of the oxidative phosphorylation system controls mitochondrial respiration in yeast
Dev Cell. 2024 Mar 18:S1534-5807(24)00110-2. doi: 10.1016/j.devcel.2024.02.011. Online ahead of print.ABSTRACTControl of protein stoichiometry is essential for cell function. Mitochondrial oxidative phosphorylation (OXPHOS) presents a complex stoichiometric challenge as the ratio of the electron transport chain (ETC) and ATP synthase must be tightly controlled, and assembly requires coordinated integration of proteins encoded in the nuclear and mitochondrial genome. How correct OXPHOS stoichiometry is achieved is unknown. We identify the MitochondrialRegulatory hub for respiratoryAssembly (MiRA) platform, which synchronize...
Source: Developmental Cell - March 20, 2024 Category: Cytology Authors: Daiana N Moretti-Horten Carlotta Peselj Asli Aras Taskin Lisa Myketin Uwe Schulte Oliver Einsle Friedel Drepper Marcin Luzarowski F-Nora V ögtle Source Type: research
Single-cell omics identifies inflammatory signaling as a trans-differentiation trigger in mouse embryos
Dev Cell. 2024 Mar 18:S1534-5807(24)00109-6. doi: 10.1016/j.devcel.2024.02.010. Online ahead of print.ABSTRACTTrans-differentiation represents a direct lineage conversion; however, insufficient characterization of this process hinders its potential applications. Here, to explore a potential universal principal for trans-differentiation, we performed single-cell transcriptomic analysis of endothelial-to-hematopoietic transition (EHT), endothelial-to-mesenchymal transition, and epithelial-to-mesenchymal transition in mouse embryos. We applied three scoring indexes of entropies, cell-type signature transcription factor expres...
Source: Developmental Cell - March 20, 2024 Category: Cytology Authors: Yifan Zhang Zhixin Kang Mengyao Liu Lu Wang Feng Liu Source Type: research
Synchronized assembly of the oxidative phosphorylation system controls mitochondrial respiration in yeast
Dev Cell. 2024 Mar 18:S1534-5807(24)00110-2. doi: 10.1016/j.devcel.2024.02.011. Online ahead of print.ABSTRACTControl of protein stoichiometry is essential for cell function. Mitochondrial oxidative phosphorylation (OXPHOS) presents a complex stoichiometric challenge as the ratio of the electron transport chain (ETC) and ATP synthase must be tightly controlled, and assembly requires coordinated integration of proteins encoded in the nuclear and mitochondrial genome. How correct OXPHOS stoichiometry is achieved is unknown. We identify the MitochondrialRegulatory hub for respiratoryAssembly (MiRA) platform, which synchronize...
Source: Developmental Cell - March 20, 2024 Category: Cytology Authors: Daiana N Moretti-Horten Carlotta Peselj Asli Aras Taskin Lisa Myketin Uwe Schulte Oliver Einsle Friedel Drepper Marcin Luzarowski F-Nora V ögtle Source Type: research
The sirtuin-associated human senescence program converges on the activation of placenta-specific gene PAPPA
Dev Cell. 2024 Mar 11:S1534-5807(24)00107-2. doi: 10.1016/j.devcel.2024.02.008. Online ahead of print.ABSTRACTSirtuins are pro-longevity genes with chromatin modulation potential, but how these properties are connected is not well understood. Here, we generated a panel of isogeneic human stem cell lines with SIRT1-SIRT7 knockouts and found that any sirtuin deficiency leads to accelerated cellular senescence. Through large-scale epigenomic analyses, we show how sirtuin deficiency alters genome organization and that genomic regions sensitive to sirtuin deficiency are preferentially enriched in active enhancers, thereby promo...
Source: Developmental Cell - March 14, 2024 Category: Cytology Authors: Shijia Bi Xiaoyu Jiang Qianzhao Ji Zehua Wang Jie Ren Si Wang Yang Yu Ruoqi Wang Zunpeng Liu Junhang Liu Jianli Hu Guoqiang Sun Zeming Wu Zhiqing Diao Jingyi Li Liang Sun Juan Carlos Izpisua Belmonte Weiqi Zhang Guang-Hui Liu Jing Qu Source Type: research
The sirtuin-associated human senescence program converges on the activation of placenta-specific gene PAPPA
Dev Cell. 2024 Mar 11:S1534-5807(24)00107-2. doi: 10.1016/j.devcel.2024.02.008. Online ahead of print.ABSTRACTSirtuins are pro-longevity genes with chromatin modulation potential, but how these properties are connected is not well understood. Here, we generated a panel of isogeneic human stem cell lines with SIRT1-SIRT7 knockouts and found that any sirtuin deficiency leads to accelerated cellular senescence. Through large-scale epigenomic analyses, we show how sirtuin deficiency alters genome organization and that genomic regions sensitive to sirtuin deficiency are preferentially enriched in active enhancers, thereby promo...
Source: Developmental Cell - March 14, 2024 Category: Cytology Authors: Shijia Bi Xiaoyu Jiang Qianzhao Ji Zehua Wang Jie Ren Si Wang Yang Yu Ruoqi Wang Zunpeng Liu Junhang Liu Jianli Hu Guoqiang Sun Zeming Wu Zhiqing Diao Jingyi Li Liang Sun Juan Carlos Izpisua Belmonte Weiqi Zhang Guang-Hui Liu Jing Qu Source Type: research
The sirtuin-associated human senescence program converges on the activation of placenta-specific gene PAPPA
Dev Cell. 2024 Mar 11:S1534-5807(24)00107-2. doi: 10.1016/j.devcel.2024.02.008. Online ahead of print.ABSTRACTSirtuins are pro-longevity genes with chromatin modulation potential, but how these properties are connected is not well understood. Here, we generated a panel of isogeneic human stem cell lines with SIRT1-SIRT7 knockouts and found that any sirtuin deficiency leads to accelerated cellular senescence. Through large-scale epigenomic analyses, we show how sirtuin deficiency alters genome organization and that genomic regions sensitive to sirtuin deficiency are preferentially enriched in active enhancers, thereby promo...
Source: Developmental Cell - March 14, 2024 Category: Cytology Authors: Shijia Bi Xiaoyu Jiang Qianzhao Ji Zehua Wang Jie Ren Si Wang Yang Yu Ruoqi Wang Zunpeng Liu Junhang Liu Jianli Hu Guoqiang Sun Zeming Wu Zhiqing Diao Jingyi Li Liang Sun Juan Carlos Izpisua Belmonte Weiqi Zhang Guang-Hui Liu Jing Qu Source Type: research
The sirtuin-associated human senescence program converges on the activation of placenta-specific gene PAPPA
Dev Cell. 2024 Mar 11:S1534-5807(24)00107-2. doi: 10.1016/j.devcel.2024.02.008. Online ahead of print.ABSTRACTSirtuins are pro-longevity genes with chromatin modulation potential, but how these properties are connected is not well understood. Here, we generated a panel of isogeneic human stem cell lines with SIRT1-SIRT7 knockouts and found that any sirtuin deficiency leads to accelerated cellular senescence. Through large-scale epigenomic analyses, we show how sirtuin deficiency alters genome organization and that genomic regions sensitive to sirtuin deficiency are preferentially enriched in active enhancers, thereby promo...
Source: Developmental Cell - March 14, 2024 Category: Cytology Authors: Shijia Bi Xiaoyu Jiang Qianzhao Ji Zehua Wang Jie Ren Si Wang Yang Yu Ruoqi Wang Zunpeng Liu Junhang Liu Jianli Hu Guoqiang Sun Zeming Wu Zhiqing Diao Jingyi Li Liang Sun Juan Carlos Izpisua Belmonte Weiqi Zhang Guang-Hui Liu Jing Qu Source Type: research
The sirtuin-associated human senescence program converges on the activation of placenta-specific gene PAPPA
Dev Cell. 2024 Mar 11:S1534-5807(24)00107-2. doi: 10.1016/j.devcel.2024.02.008. Online ahead of print.ABSTRACTSirtuins are pro-longevity genes with chromatin modulation potential, but how these properties are connected is not well understood. Here, we generated a panel of isogeneic human stem cell lines with SIRT1-SIRT7 knockouts and found that any sirtuin deficiency leads to accelerated cellular senescence. Through large-scale epigenomic analyses, we show how sirtuin deficiency alters genome organization and that genomic regions sensitive to sirtuin deficiency are preferentially enriched in active enhancers, thereby promo...
Source: Developmental Cell - March 14, 2024 Category: Cytology Authors: Shijia Bi Xiaoyu Jiang Qianzhao Ji Zehua Wang Jie Ren Si Wang Yang Yu Ruoqi Wang Zunpeng Liu Junhang Liu Jianli Hu Guoqiang Sun Zeming Wu Zhiqing Diao Jingyi Li Liang Sun Juan Carlos Izpisua Belmonte Weiqi Zhang Guang-Hui Liu Jing Qu Source Type: research
