Aberrant cortex contractions impact mammalian oocyte quality
Dev Cell. 2024 Feb 20:S1534-5807(24)00047-9. doi: 10.1016/j.devcel.2024.01.027. Online ahead of print.ABSTRACTThe cortex controls cell shape. In mouse oocytes, the cortex thickens in an Arp2/3-complex-dependent manner, ensuring chromosome positioning and segregation. Surprisingly, we identify that mouse oocytes lacking the Arp2/3 complex undergo cortical actin remodeling upon division, followed by cortical contractions that are unprecedented in mammalian oocytes. Using genetics, imaging, and machine learning, we show that these contractions stir the cytoplasm, resulting in impaired organelle organization and activity. Oocy...
Source: Developmental Cell - February 22, 2024 Category: Cytology Authors: Elvira Nikalayevich Ga ëlle Letort Ghislain de Labbey Elena Todisco Anastasia Shihabi Herv é Turlier Rapha ël Voituriez Mohamed Yahiatene Xavier Pollet-Villard Metello Innocenti Melina Schuh Marie-Emilie Terret Marie-H élène Verlhac Source Type: research

ATG5 attenuates inflammatory signaling in mouse embryonic stem cells to control differentiation
Dev Cell. 2024 Feb 14:S1534-5807(24)00046-7. doi: 10.1016/j.devcel.2024.01.026. Online ahead of print.ABSTRACTAttenuated inflammatory response is a property of embryonic stem cells (ESCs). However, the underlying mechanisms are unclear. Moreover, whether the attenuated inflammatory status is involved in ESC differentiation is also unknown. Here, we found that autophagy-related protein ATG5 is essential for both attenuated inflammatory response and differentiation of mouse ESCs and that attenuation of inflammatory signaling is required for mouse ESC differentiation. Mechanistically, ATG5 recruits FBXW7 to promote ubiquitina...
Source: Developmental Cell - February 22, 2024 Category: Cytology Authors: Sheng Li Jin Sun Bo-Wen Zhang Lu Yang Ying-Cui Wan Bei-Bei Chen Nan Xu Qian-Ru Xu Juan Fan Jia-Ni Shang Rui Li Chen-Ge Yu Yan Xi Su Chen Source Type: research

Aberrant cortex contractions impact mammalian oocyte quality
Dev Cell. 2024 Feb 20:S1534-5807(24)00047-9. doi: 10.1016/j.devcel.2024.01.027. Online ahead of print.ABSTRACTThe cortex controls cell shape. In mouse oocytes, the cortex thickens in an Arp2/3-complex-dependent manner, ensuring chromosome positioning and segregation. Surprisingly, we identify that mouse oocytes lacking the Arp2/3 complex undergo cortical actin remodeling upon division, followed by cortical contractions that are unprecedented in mammalian oocytes. Using genetics, imaging, and machine learning, we show that these contractions stir the cytoplasm, resulting in impaired organelle organization and activity. Oocy...
Source: Developmental Cell - February 22, 2024 Category: Cytology Authors: Elvira Nikalayevich Ga ëlle Letort Ghislain de Labbey Elena Todisco Anastasia Shihabi Herv é Turlier Rapha ël Voituriez Mohamed Yahiatene Xavier Pollet-Villard Metello Innocenti Melina Schuh Marie-Emilie Terret Marie-H élène Verlhac Source Type: research

ATG5 attenuates inflammatory signaling in mouse embryonic stem cells to control differentiation
Dev Cell. 2024 Feb 14:S1534-5807(24)00046-7. doi: 10.1016/j.devcel.2024.01.026. Online ahead of print.ABSTRACTAttenuated inflammatory response is a property of embryonic stem cells (ESCs). However, the underlying mechanisms are unclear. Moreover, whether the attenuated inflammatory status is involved in ESC differentiation is also unknown. Here, we found that autophagy-related protein ATG5 is essential for both attenuated inflammatory response and differentiation of mouse ESCs and that attenuation of inflammatory signaling is required for mouse ESC differentiation. Mechanistically, ATG5 recruits FBXW7 to promote ubiquitina...
Source: Developmental Cell - February 22, 2024 Category: Cytology Authors: Sheng Li Jin Sun Bo-Wen Zhang Lu Yang Ying-Cui Wan Bei-Bei Chen Nan Xu Qian-Ru Xu Juan Fan Jia-Ni Shang Rui Li Chen-Ge Yu Yan Xi Su Chen Source Type: research

Stem cell migration drives lung repair in living mice
Dev Cell. 2024 Feb 14:S1534-5807(24)00074-1. doi: 10.1016/j.devcel.2024.02.003. Online ahead of print.ABSTRACTTissue repair requires a highly coordinated cellular response to injury. In the lung, alveolar type 2 cells (AT2s) act as stem cells to replenish both themselves and alveolar type 1 cells (AT1s); however, the complex orchestration of stem cell activity after injury is poorly understood. Here, we establish longitudinal imaging of AT2s in murine intact tissues ex vivo and in vivo in order to track their dynamic behavior over time. We discover that a large fraction of AT2s become motile following injury and provide di...
Source: Developmental Cell - February 20, 2024 Category: Cytology Authors: Maurizio Chioccioli Shuyu Liu Sumner Magruder Aleksandra Tata Lucia Borriello John E McDonough Arvind Konkimalla Sang-Hun Kim Jessica Nouws David G Gonzalez Brian Traub Xianjun Ye Tao Yang David R Entenberg Smita Krishnaswamy Caroline E Hendry Naftali Kam Source Type: research

Stem cell migration drives lung repair in living mice
Dev Cell. 2024 Feb 14:S1534-5807(24)00074-1. doi: 10.1016/j.devcel.2024.02.003. Online ahead of print.ABSTRACTTissue repair requires a highly coordinated cellular response to injury. In the lung, alveolar type 2 cells (AT2s) act as stem cells to replenish both themselves and alveolar type 1 cells (AT1s); however, the complex orchestration of stem cell activity after injury is poorly understood. Here, we establish longitudinal imaging of AT2s in murine intact tissues ex vivo and in vivo in order to track their dynamic behavior over time. We discover that a large fraction of AT2s become motile following injury and provide di...
Source: Developmental Cell - February 20, 2024 Category: Cytology Authors: Maurizio Chioccioli Shuyu Liu Sumner Magruder Aleksandra Tata Lucia Borriello John E McDonough Arvind Konkimalla Sang-Hun Kim Jessica Nouws David G Gonzalez Brian Traub Xianjun Ye Tao Yang David R Entenberg Smita Krishnaswamy Caroline E Hendry Naftali Kam Source Type: research

SLAM-ITseq identifies that Nrf2 induces liver regeneration through the pentose phosphate pathway
Dev Cell. 2024 Feb 12:S1534-5807(24)00044-3. doi: 10.1016/j.devcel.2024.01.024. Online ahead of print.ABSTRACTThe liver exhibits a remarkable capacity to regenerate following injury. Despite this unique attribute, toxic injury is a leading cause of liver failure. The temporal processes by which the liver senses injury and initiates regeneration remain unclear. Here, we developed a transgenic zebrafish model wherein hepatocyte-specific expression of uracil phosphoribosyltransferase (UPRT) enabled the implementation of SLAM-ITseq to investigate the nascent transcriptome during initiation of liver injury and regeneration. Usi...
Source: Developmental Cell - February 17, 2024 Category: Cytology Authors: Vicky W T Tan Talhah M Salmi Anthony P Karamalakis Andrea Gillespie Athena Jessica S Ong Jesse J Balic Yih-Chih Chan Cerys E Bladen Kristin K Brown Mark A Dawson Andrew G Cox Source Type: research

A metabolically controlled contact site between vacuoles and lipid droplets in yeast
Dev Cell. 2024 Feb 16:S1534-5807(24)00036-4. doi: 10.1016/j.devcel.2024.01.016. Online ahead of print.ABSTRACTThe lipid droplet (LD) organization proteins Ldo16 and Ldo45 affect multiple aspects of LD biology in yeast. They are linked to the LD biogenesis machinery seipin, and their loss causes defects in LD positioning, protein targeting, and breakdown. However, their molecular roles remained enigmatic. Here, we report that Ldo16/45 form a tether complex with Vac8 to create vacuole lipid droplet (vCLIP) contact sites, which can form in the absence of seipin. The phosphatidylinositol transfer protein (PITP) Pdr16 is a furt...
Source: Developmental Cell - February 17, 2024 Category: Cytology Authors: Duy Trong Vien Diep Javier Collado Marie Hugenroth Rebecca Martina Fausten Louis Percifull Mike W älte Christian Schuberth Oliver Schmidt Rub én Fernández-Busnadiego Maria Bohnert Source Type: research

SLAM-ITseq identifies that Nrf2 induces liver regeneration through the pentose phosphate pathway
Dev Cell. 2024 Feb 12:S1534-5807(24)00044-3. doi: 10.1016/j.devcel.2024.01.024. Online ahead of print.ABSTRACTThe liver exhibits a remarkable capacity to regenerate following injury. Despite this unique attribute, toxic injury is a leading cause of liver failure. The temporal processes by which the liver senses injury and initiates regeneration remain unclear. Here, we developed a transgenic zebrafish model wherein hepatocyte-specific expression of uracil phosphoribosyltransferase (UPRT) enabled the implementation of SLAM-ITseq to investigate the nascent transcriptome during initiation of liver injury and regeneration. Usi...
Source: Developmental Cell - February 17, 2024 Category: Cytology Authors: Vicky W T Tan Talhah M Salmi Anthony P Karamalakis Andrea Gillespie Athena Jessica S Ong Jesse J Balic Yih-Chih Chan Cerys E Bladen Kristin K Brown Mark A Dawson Andrew G Cox Source Type: research

A metabolically controlled contact site between vacuoles and lipid droplets in yeast
Dev Cell. 2024 Feb 16:S1534-5807(24)00036-4. doi: 10.1016/j.devcel.2024.01.016. Online ahead of print.ABSTRACTThe lipid droplet (LD) organization proteins Ldo16 and Ldo45 affect multiple aspects of LD biology in yeast. They are linked to the LD biogenesis machinery seipin, and their loss causes defects in LD positioning, protein targeting, and breakdown. However, their molecular roles remained enigmatic. Here, we report that Ldo16/45 form a tether complex with Vac8 to create vacuole lipid droplet (vCLIP) contact sites, which can form in the absence of seipin. The phosphatidylinositol transfer protein (PITP) Pdr16 is a furt...
Source: Developmental Cell - February 17, 2024 Category: Cytology Authors: Duy Trong Vien Diep Javier Collado Marie Hugenroth Rebecca Martina Fausten Louis Percifull Mike W älte Christian Schuberth Oliver Schmidt Rub én Fernández-Busnadiego Maria Bohnert Source Type: research

SLAM-ITseq identifies that Nrf2 induces liver regeneration through the pentose phosphate pathway
Dev Cell. 2024 Feb 12:S1534-5807(24)00044-3. doi: 10.1016/j.devcel.2024.01.024. Online ahead of print.ABSTRACTThe liver exhibits a remarkable capacity to regenerate following injury. Despite this unique attribute, toxic injury is a leading cause of liver failure. The temporal processes by which the liver senses injury and initiates regeneration remain unclear. Here, we developed a transgenic zebrafish model wherein hepatocyte-specific expression of uracil phosphoribosyltransferase (UPRT) enabled the implementation of SLAM-ITseq to investigate the nascent transcriptome during initiation of liver injury and regeneration. Usi...
Source: Developmental Cell - February 17, 2024 Category: Cytology Authors: Vicky W T Tan Talhah M Salmi Anthony P Karamalakis Andrea Gillespie Athena Jessica S Ong Jesse J Balic Yih-Chih Chan Cerys E Bladen Kristin K Brown Mark A Dawson Andrew G Cox Source Type: research

A metabolically controlled contact site between vacuoles and lipid droplets in yeast
Dev Cell. 2024 Feb 16:S1534-5807(24)00036-4. doi: 10.1016/j.devcel.2024.01.016. Online ahead of print.ABSTRACTThe lipid droplet (LD) organization proteins Ldo16 and Ldo45 affect multiple aspects of LD biology in yeast. They are linked to the LD biogenesis machinery seipin, and their loss causes defects in LD positioning, protein targeting, and breakdown. However, their molecular roles remained enigmatic. Here, we report that Ldo16/45 form a tether complex with Vac8 to create vacuole lipid droplet (vCLIP) contact sites, which can form in the absence of seipin. The phosphatidylinositol transfer protein (PITP) Pdr16 is a furt...
Source: Developmental Cell - February 17, 2024 Category: Cytology Authors: Duy Trong Vien Diep Javier Collado Marie Hugenroth Rebecca Martina Fausten Louis Percifull Mike W älte Christian Schuberth Oliver Schmidt Rub én Fernández-Busnadiego Maria Bohnert Source Type: research

SLAM-ITseq identifies that Nrf2 induces liver regeneration through the pentose phosphate pathway
Dev Cell. 2024 Feb 12:S1534-5807(24)00044-3. doi: 10.1016/j.devcel.2024.01.024. Online ahead of print.ABSTRACTThe liver exhibits a remarkable capacity to regenerate following injury. Despite this unique attribute, toxic injury is a leading cause of liver failure. The temporal processes by which the liver senses injury and initiates regeneration remain unclear. Here, we developed a transgenic zebrafish model wherein hepatocyte-specific expression of uracil phosphoribosyltransferase (UPRT) enabled the implementation of SLAM-ITseq to investigate the nascent transcriptome during initiation of liver injury and regeneration. Usi...
Source: Developmental Cell - February 17, 2024 Category: Cytology Authors: Vicky W T Tan Talhah M Salmi Anthony P Karamalakis Andrea Gillespie Athena Jessica S Ong Jesse J Balic Yih-Chih Chan Cerys E Bladen Kristin K Brown Mark A Dawson Andrew G Cox Source Type: research

Gibberellin acid promotes single-celled fiber elongation through the activation of two signaling cascades in cotton
Dev Cell. 2024 Feb 6:S1534-5807(24)00038-8. doi: 10.1016/j.devcel.2024.01.018. Online ahead of print.ABSTRACTThe agricultural green revolution spectacularly enhanced crop yield through modification of gibberellin (GA) signaling. However, in cotton, the GA signaling cascades remain elusive, limiting our potential to cultivate new cotton varieties and improve yield and quality. Here, we identified that GA prominently stimulated fiber elongation through the degradation of DELLA protein GhSLR1, thereby disabling GhSLR1's physical interaction with two transcription factors, GhZFP8 and GhBLH1. Subsequently, the resultant free Gh...
Source: Developmental Cell - February 15, 2024 Category: Cytology Authors: Peng He Liping Zhu Xin Zhou Xuan Fu Yu Zhang Peng Zhao Bin Jiang Huiqin Wang Guanghui Xiao Source Type: research

Lineage motifs as developmental modules for control of cell type proportions
Dev Cell. 2024 Feb 6:S1534-5807(24)00037-6. doi: 10.1016/j.devcel.2024.01.017. Online ahead of print.ABSTRACTIn multicellular organisms, cell types must be produced and maintained in appropriate proportions. One way this is achieved is through committed progenitor cells or extrinsic interactions that produce specific patterns of descendant cell types on lineage trees. However, cell fate commitment is probabilistic in most contexts, making it difficult to infer these dynamics and understand how they establish overall cell type proportions. Here, we introduce Lineage Motif Analysis (LMA), a method that recursively identifies...
Source: Developmental Cell - February 15, 2024 Category: Cytology Authors: Martin Tran Amjad Askary Michael B Elowitz Source Type: research