COPII with ALG2 and ESCRTs control lysosome-dependent microautophagy of ER exit sites
Dev Cell. 2024 Apr 6:S1534-5807(24)00195-3. doi: 10.1016/j.devcel.2024.03.027. Online ahead of print.ABSTRACTEndoplasmic reticulum exit sites (ERESs) are tubular outgrowths of endoplasmic reticulum that serve as the earliest station for protein sorting and export into the secretory pathway. How these structures respond to different cellular conditions remains unclear. Here, we report that ERESs undergo lysosome-dependent microautophagy when Ca2+ is released by lysosomes in response to nutrient stressors such as mTOR inhibition or amino acid starvation in mammalian cells. Targeting and uptake of ERESs into lysosomes were ob...
Source: Developmental Cell - April 9, 2024 Category: Cytology Authors: Ya-Cheng Liao Song Pang Wei-Ping Li Gleb Shtengel Heejun Choi Kathy Schaefer C Shan Xu Jennifer Lippincott-Schwartz Source Type: research
No need to "sugar coat": Removing glycocalyx on apoptotic blebs promotes phagocytosis
Dev Cell. 2024 Apr 8;59(7):827-829. doi: 10.1016/j.devcel.2024.02.014.ABSTRACTThe viscous glycocalyx of mammalian cells, composed of glucosaminoglycans, glycolipids, and glycoproteins, "sugar coat" the outer plasma membrane. In this issue of Developmental Cell, Le et al. (2024) show that the glycocalyx is removed from apoptotic blebs via disassembly of the cortical cytoskeleton, exposing the "eat-me" signals necessary for efferocytosis.PMID:38593785 | DOI:10.1016/j.devcel.2024.02.014 (Source: Developmental Cell)
Source: Developmental Cell - April 9, 2024 Category: Cytology Authors: Shannon M Kelley Kodi S Ravichandran Source Type: research
3D reconstruction of the mouse cochlea from scRNA-seq data suggests morphogen-based principles in apex-to-base specification
Dev Cell. 2024 Apr 4:S1534-5807(24)00196-5. doi: 10.1016/j.devcel.2024.03.028. Online ahead of print.ABSTRACTIn the mammalian auditory system, frequency discrimination depends on numerous morphological and physiological properties of the organ of Corti, which gradually change along the apex-to-base (tonotopic) axis of the organ. For example, the basilar membrane stiffness changes tonotopically, thus affecting the tuning properties of individual hair cells. At the molecular level, those frequency-specific characteristics are mirrored by gene expression gradients; however, the molecular mechanisms controlling tonotopic gene ...
Source: Developmental Cell - April 9, 2024 Category: Cytology Authors: Shuze Wang Saikat Chakraborty Yujuan Fu Mary P Lee Jie Liu Joerg Waldhaus Source Type: research
OsMADS6-OsMADS32 and REP1 control palea cellular heterogeneity and morphogenesis in rice
We report that the transcription factor (TF) REP1 promotes epidermal cell proliferation and differentiation in the BOP, resulting in hard silicified protrusion cells, by regulating the cyclin-dependent kinase gene, OsCDKB1;1. Conversely, TFs OsMADS6 and OsMADS32 are expressed exclusively in the MRP, where they limit cell division rates by inhibiting OsCDKB2;1 expression and promote endoreduplication, yielding elongated epidermal cells. Furthermore, reciprocal inhibition between the OsMADS6-OsMADS32 complex and REP1 fine-tunes the balance between cell division and differentiation during palea morphogenesis. We further show ...
Source: Developmental Cell - April 9, 2024 Category: Cytology Authors: Xuelian Zhang Qiang Cai Ling Li Li Wang Yun Hu Xiaofei Chen Dabing Zhang Staffan Persson Zheng Yuan Source Type: research
COPII with ALG2 and ESCRTs control lysosome-dependent microautophagy of ER exit sites
Dev Cell. 2024 Apr 6:S1534-5807(24)00195-3. doi: 10.1016/j.devcel.2024.03.027. Online ahead of print.ABSTRACTEndoplasmic reticulum exit sites (ERESs) are tubular outgrowths of endoplasmic reticulum that serve as the earliest station for protein sorting and export into the secretory pathway. How these structures respond to different cellular conditions remains unclear. Here, we report that ERESs undergo lysosome-dependent microautophagy when Ca2+ is released by lysosomes in response to nutrient stressors such as mTOR inhibition or amino acid starvation in mammalian cells. Targeting and uptake of ERESs into lysosomes were ob...
Source: Developmental Cell - April 9, 2024 Category: Cytology Authors: Ya-Cheng Liao Song Pang Wei-Ping Li Gleb Shtengel Heejun Choi Kathy Schaefer C Shan Xu Jennifer Lippincott-Schwartz Source Type: research
SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements
Dev Cell. 2024 Apr 5:S1534-5807(24)00193-X. doi: 10.1016/j.devcel.2024.03.025. Online ahead of print.ABSTRACTThe commitment and differentiation of human placental progenitor cytotrophoblast (CT) cells are crucial for a successful pregnancy, but the underlying mechanism remains poorly understood. Here, we identified the transcription factor (TF), specificity protein 6 (SP6), as a human species-specific trophoblast lineage TF expressed in human placental CT cells. Using pluripotent stem cells as a model, we demonstrated that SP6 controls CT generation and the establishment of trophoblast stem cells (TSCs) and identified msh ...
Source: Developmental Cell - April 6, 2024 Category: Cytology Authors: Yanglin Chen Xianhua Ye Yulong Zhong Xiangjin Kang Yanqing Tang Haoyun Zhu Changmiao Pang Shaoqiang Ning Shiqing Liang Feifan Zhang Chao Li Jie Li Chengtao Gu Yuanxiong Cheng Zhanpeng Kuang Jingyang Qiu Jin Jin Haisi Luo Mingyu Fu Hannah Xiaoyan Hui Lei L Source Type: research
SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements
Dev Cell. 2024 Apr 5:S1534-5807(24)00193-X. doi: 10.1016/j.devcel.2024.03.025. Online ahead of print.ABSTRACTThe commitment and differentiation of human placental progenitor cytotrophoblast (CT) cells are crucial for a successful pregnancy, but the underlying mechanism remains poorly understood. Here, we identified the transcription factor (TF), specificity protein 6 (SP6), as a human species-specific trophoblast lineage TF expressed in human placental CT cells. Using pluripotent stem cells as a model, we demonstrated that SP6 controls CT generation and the establishment of trophoblast stem cells (TSCs) and identified msh ...
Source: Developmental Cell - April 6, 2024 Category: Cytology Authors: Yanglin Chen Xianhua Ye Yulong Zhong Xiangjin Kang Yanqing Tang Haoyun Zhu Changmiao Pang Shaoqiang Ning Shiqing Liang Feifan Zhang Chao Li Jie Li Chengtao Gu Yuanxiong Cheng Zhanpeng Kuang Jingyang Qiu Jin Jin Haisi Luo Mingyu Fu Hannah Xiaoyan Hui Lei L Source Type: research
SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements
Dev Cell. 2024 Apr 5:S1534-5807(24)00193-X. doi: 10.1016/j.devcel.2024.03.025. Online ahead of print.ABSTRACTThe commitment and differentiation of human placental progenitor cytotrophoblast (CT) cells are crucial for a successful pregnancy, but the underlying mechanism remains poorly understood. Here, we identified the transcription factor (TF), specificity protein 6 (SP6), as a human species-specific trophoblast lineage TF expressed in human placental CT cells. Using pluripotent stem cells as a model, we demonstrated that SP6 controls CT generation and the establishment of trophoblast stem cells (TSCs) and identified msh ...
Source: Developmental Cell - April 6, 2024 Category: Cytology Authors: Yanglin Chen Xianhua Ye Yulong Zhong Xiangjin Kang Yanqing Tang Haoyun Zhu Changmiao Pang Shaoqiang Ning Shiqing Liang Feifan Zhang Chao Li Jie Li Chengtao Gu Yuanxiong Cheng Zhanpeng Kuang Jingyang Qiu Jin Jin Haisi Luo Mingyu Fu Hannah Xiaoyan Hui Lei L Source Type: research
Oct4 controls basement membrane development during human embryogenesis
Dev Cell. 2024 Apr 1:S1534-5807(24)00175-8. doi: 10.1016/j.devcel.2024.03.007. Online ahead of print.ABSTRACTBasement membranes (BMs) are sheet-like structures of extracellular matrix (ECM) that provide structural support for many tissues and play a central role in signaling. They are key regulators of cell behavior and tissue functions, and defects in their assembly or composition are involved in numerous human diseases. Due to the differences between human and animal embryogenesis, ethical concerns, legal constraints, the scarcity of human tissue material, and the inaccessibility of the in vivo condition, BM regulation d...
Source: Developmental Cell - April 5, 2024 Category: Cytology Authors: Margit Rosner Markus Hengstschl äger Source Type: research
Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization
Dev Cell. 2024 Apr 2:S1534-5807(24)00177-1. doi: 10.1016/j.devcel.2024.03.009. Online ahead of print.ABSTRACTPlant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predi...
Source: Developmental Cell - April 5, 2024 Category: Cytology Authors: Lukas Hoermayer Juan Carlos Montesinos Nicola Trozzi Leonhard Spona Saiko Yoshida Petra Marhava Silvia Caballero-Mancebo Eva Benkov á Carl-Philip Heisenberg Yasin Dagdas Mateusz Majda Ji ří Friml Source Type: research
Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoids
Dev Cell. 2024 Apr 3:S1534-5807(24)00192-8. doi: 10.1016/j.devcel.2024.03.024. Online ahead of print.ABSTRACTEmbryogenesis requires substantial coordination to translate genetic programs to the collective behavior of differentiating cells, but understanding how cellular decisions control tissue morphology remains conceptually and technically challenging. Here, we combine continuous Cas9-based molecular recording with a mouse embryonic stem cell-based model of the embryonic trunk to build single-cell phylogenies that describe the behavior of transient, multipotent neuro-mesodermal progenitors (NMPs) as they commit into neur...
Source: Developmental Cell - April 5, 2024 Category: Cytology Authors: Adriano Bolondi Benjamin K Law Helene Kretzmer Seher Ipek Gassaloglu Ren é Buschow Christina Riemenschneider Dian Yang Maria Walther Jesse V Veenvliet Alexander Meissner Zachary D Smith Michelle M Chan Source Type: research
A peroxisomal cinnamate:CoA ligase-dependent phytohormone metabolic cascade in submerged rice germination
Dev Cell. 2024 Apr 2:S1534-5807(24)00191-6. doi: 10.1016/j.devcel.2024.03.023. Online ahead of print.ABSTRACTThe mechanism underlying the ability of rice to germinate underwater is a largely enigmatic but key research question highly relevant to rice cultivation. Moreover, although rice is known to accumulate salicylic acid (SA), SA biosynthesis is poorly defined, and its role in underwater germination is unknown. It is also unclear whether peroxisomes, organelles essential to oilseed germination and rice SA accumulation, play a role in rice germination. Here, we show that submerged imbibition of rice seeds induces SA accu...
Source: Developmental Cell - April 5, 2024 Category: Cytology Authors: Yukang Wang Gaochen Jin Shuyan Song Yijun Jin Xiaowen Wang Shuaiqi Yang Xingxing Shen Yinbo Gan Yuexing Wang Ran Li Jian-Xiang Liu Jianping Hu Ronghui Pan Source Type: research
Basal delamination during mouse gastrulation primes pluripotent cells for differentiation
Dev Cell. 2024 Apr 2:S1534-5807(24)00176-X. doi: 10.1016/j.devcel.2024.03.008. Online ahead of print.ABSTRACTThe blueprint of the mammalian body plan is laid out during gastrulation, when a trilaminar embryo is formed. This process entails a burst of proliferation, the ingression of embryonic epiblast cells at the primitive streak, and their priming toward primitive streak fates. How these different events are coordinated remains unknown. Here, we developed and characterized a 3D culture of self-renewing mouse embryonic cells that captures the main transcriptional and architectural features of the early gastrulating mouse ...
Source: Developmental Cell - April 5, 2024 Category: Cytology Authors: Nanami Sato Viviane S Rosa Aly Makhlouf Helene Kretzmer Abhishek Sampath Kumar Stefanie Grosswendt Alexandra L Mattei Olivia Courbot Steffen Wolf Jerome Boulanger Frederic Langevin Michal Wiacek Daniel Karpinski Alberto Elosegui-Artola Alexander Meissner Source Type: research
Bifunctional transcription factors SlERF.H5 and H7 activate cell wall and repress gibberellin biosynthesis genes in tomato via a conserved motif
Dev Cell. 2024 Apr 2:S1534-5807(24)00174-6. doi: 10.1016/j.devcel.2024.03.006. Online ahead of print.ABSTRACTThe plant cell wall is a dynamic structure that plays an essential role in development, but the mechanism regulating cell wall formation remains poorly understood. We demonstrate that two transcription factors, SlERF.H5 and SlERF.H7, control cell wall formation and tomato fruit firmness in an additive manner. Knockout of SlERF.H5, SlERF.H7, or both genes decreased cell wall thickness, firmness, and cellulose contents in fruits during early development, especially in double-knockout lines. Overexpressing either gene ...
Source: Developmental Cell - April 5, 2024 Category: Cytology Authors: Yangang Pei Qihan Xue Peng Shu Weijie Xu Xiaofei Du Mengbo Wu Kaidong Liu Julien Pirrello Mondher Bouzayen Yiguo Hong Mingchun Liu Source Type: research
Oct4 controls basement membrane development during human embryogenesis
Dev Cell. 2024 Apr 1:S1534-5807(24)00175-8. doi: 10.1016/j.devcel.2024.03.007. Online ahead of print.ABSTRACTBasement membranes (BMs) are sheet-like structures of extracellular matrix (ECM) that provide structural support for many tissues and play a central role in signaling. They are key regulators of cell behavior and tissue functions, and defects in their assembly or composition are involved in numerous human diseases. Due to the differences between human and animal embryogenesis, ethical concerns, legal constraints, the scarcity of human tissue material, and the inaccessibility of the in vivo condition, BM regulation d...
Source: Developmental Cell - April 5, 2024 Category: Cytology Authors: Margit Rosner Markus Hengstschl äger Source Type: research