Sweat gland development requires an eccrine dermal niche and couples two epidermal programs
Dev Cell. 2023 Dec 7:S1534-5807(23)00613-5. doi: 10.1016/j.devcel.2023.11.015. Online ahead of print.ABSTRACTEccrine sweat glands are indispensable for human thermoregulation and, similar to other mammalian skin appendages, form from multipotent epidermal progenitors. Limited understanding of how epidermal progenitors specialize to form these vital organs has precluded therapeutic efforts toward their regeneration. Herein, we applied single-nucleus transcriptomics to compare the expression content of wild-type, eccrine-forming mouse skin to that of mice harboring a skin-specific disruption of Engrailed 1 (En1), a transcrip...
Source: Developmental Cell - December 14, 2023 Category: Cytology Authors: Heather L Dingwall Reiko R Tomizawa Adam Aharoni Peng Hu Qi Qiu Blerina Kokalari Serenity M Martinez Joan C Donahue Daniel Aldea Meryl Mendoza Ian A Glass Birth Defects Research Laboratory (BDRL) Hao Wu Yana G Kamberov Source Type: research
Eclosion muscles secrete ecdysteroids to initiate asymmetric intestinal stem cell division in Drosophila
Dev Cell. 2023 Dec 8:S1534-5807(23)00614-7. doi: 10.1016/j.devcel.2023.11.016. Online ahead of print.ABSTRACTDuring organ development, tissue stem cells first expand via symmetric divisions and then switch to asymmetric divisions to minimize the time to obtain a mature tissue. In the Drosophila midgut, intestinal stem cells switch their divisions from symmetric to asymmetric at midpupal development to produce enteroendocrine cells. However, the signals that initiate this switch are unknown. Here, we identify the signal as ecdysteroids. In the presence of ecdysone, EcR and Usp promote the expression of E93 to suppress Br ex...
Source: Developmental Cell - December 14, 2023 Category: Cytology Authors: Song Zhang Song Wu Ruining Yao Xueying Wei Benjamin Ohlstein Zheng Guo Source Type: research
Sweat gland development requires an eccrine dermal niche and couples two epidermal programs
Dev Cell. 2023 Dec 7:S1534-5807(23)00613-5. doi: 10.1016/j.devcel.2023.11.015. Online ahead of print.ABSTRACTEccrine sweat glands are indispensable for human thermoregulation and, similar to other mammalian skin appendages, form from multipotent epidermal progenitors. Limited understanding of how epidermal progenitors specialize to form these vital organs has precluded therapeutic efforts toward their regeneration. Herein, we applied single-nucleus transcriptomics to compare the expression content of wild-type, eccrine-forming mouse skin to that of mice harboring a skin-specific disruption of Engrailed 1 (En1), a transcrip...
Source: Developmental Cell - December 14, 2023 Category: Cytology Authors: Heather L Dingwall Reiko R Tomizawa Adam Aharoni Peng Hu Qi Qiu Blerina Kokalari Serenity M Martinez Joan C Donahue Daniel Aldea Meryl Mendoza Ian A Glass Birth Defects Research Laboratory (BDRL) Hao Wu Yana G Kamberov Source Type: research
HOPX-associated molecular programs control cardiomyocyte cell states underpinning cardiac structure and function
This study uses genetic loss-of-function human-induced pluripotent stem cell-derived cardiomyocytes to evaluate the genomic regulatory basis of the non-DNA-binding homeodomain protein HOPX. We show that HOPX interacts with and controls cardiac genes and enhancer networks associated with diverse aspects of heart development. Using perturbation studies in vitro, we define how upstream cell growth and proliferation control HOPX transcription to regulate cardiac gene programs. We then use cell, organoid, and zebrafish regeneration models to demonstrate that HOPX-regulated gene programs control cardiomyocyte function in develop...
Source: Developmental Cell - December 13, 2023 Category: Cytology Authors: Clayton E Friedman Seth W Cheetham Sumedha Negi Richard J Mills Masahito Ogawa Meredith A Redd Han Sheng Chiu Sophie Shen Yuliangzi Sun Dalia Mizikovsky Romaric Bouveret Xiaoli Chen Holly K Voges Scott Paterson Jessica E De Angelis Stacey B Andersen Yuanz Source Type: research
Two-phase kinetics and cell cortex elastic behavior in Xenopus gastrula cell-cell adhesion
Dev Cell. 2023 Dec 5:S1534-5807(23)00612-3. doi: 10.1016/j.devcel.2023.11.014. Online ahead of print.ABSTRACTMorphogenetic movements during animal development involve repeated making and breaking of cell-cell contacts. Recent biophysical models of cell-cell adhesion integrate adhesion molecule interactions and cortical cytoskeletal tension modulation, describing equilibrium states for established contacts. We extend this emerging unified concept of adhesion to contact formation kinetics, showing that aggregating Xenopus embryonic cells rapidly achieve Ca2+-independent low-contact states. Subsequent transitions to cadherin-...
Source: Developmental Cell - December 13, 2023 Category: Cytology Authors: Serge E Parent Olivia Luu Ashley E E Bruce Rudolf Winklbauer Source Type: research
HOPX-associated molecular programs control cardiomyocyte cell states underpinning cardiac structure and function
This study uses genetic loss-of-function human-induced pluripotent stem cell-derived cardiomyocytes to evaluate the genomic regulatory basis of the non-DNA-binding homeodomain protein HOPX. We show that HOPX interacts with and controls cardiac genes and enhancer networks associated with diverse aspects of heart development. Using perturbation studies in vitro, we define how upstream cell growth and proliferation control HOPX transcription to regulate cardiac gene programs. We then use cell, organoid, and zebrafish regeneration models to demonstrate that HOPX-regulated gene programs control cardiomyocyte function in develop...
Source: Developmental Cell - December 13, 2023 Category: Cytology Authors: Clayton E Friedman Seth W Cheetham Sumedha Negi Richard J Mills Masahito Ogawa Meredith A Redd Han Sheng Chiu Sophie Shen Yuliangzi Sun Dalia Mizikovsky Romaric Bouveret Xiaoli Chen Holly K Voges Scott Paterson Jessica E De Angelis Stacey B Andersen Yuanz Source Type: research
Two-phase kinetics and cell cortex elastic behavior in Xenopus gastrula cell-cell adhesion
Dev Cell. 2023 Dec 5:S1534-5807(23)00612-3. doi: 10.1016/j.devcel.2023.11.014. Online ahead of print.ABSTRACTMorphogenetic movements during animal development involve repeated making and breaking of cell-cell contacts. Recent biophysical models of cell-cell adhesion integrate adhesion molecule interactions and cortical cytoskeletal tension modulation, describing equilibrium states for established contacts. We extend this emerging unified concept of adhesion to contact formation kinetics, showing that aggregating Xenopus embryonic cells rapidly achieve Ca2+-independent low-contact states. Subsequent transitions to cadherin-...
Source: Developmental Cell - December 13, 2023 Category: Cytology Authors: Serge E Parent Olivia Luu Ashley E E Bruce Rudolf Winklbauer Source Type: research
An Arabidopsis Rab18 GTPase promotes autophagy by tethering ATG18a to the ER in response to nutrient starvation
This study finds that ATG18a acts as a downstream effector of RABC1, a member of the poorly characterized Rab18/RabC GTPase subclass in plants. Active RABC1 interacts with ATG18a on the ER, particularly under nutrient starvation. In rabc1 mutants, autophagy is compromised, especially under nutrient deprivation, affecting the ER association and expansion of ATG18a-positive autophagosomes. Furthermore, both dominant-negative and constitutively active RABC1 forms inhibit autophagy. The dominant inactive RABC1 impedes the ER association of ATG18a, whereas the constitutively active RABC1 delays ATG18a detachment from the ER. Co...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: Jiaqi Sun Yang Shao Songyang Wang Xunzheng Li Shuqing Feng Weina Wang Pierre Leroy Chengyang Li Huanquan Zheng Source Type: research
Murine trophoblast organoids as a model for trophoblast development and CRISPR-Cas9 screening
Dev Cell. 2023 Nov 24:S1534-5807(23)00584-1. doi: 10.1016/j.devcel.2023.11.007. Online ahead of print.ABSTRACTThe placenta becomes one of the most diversified organs during placental mammal radiation. The main in vitro model for studying mouse trophoblast development is the 2D differentiation model of trophoblast stem cells, which is highly skewed to certain lineages and thus hampers systematic screens. Here, we established culture conditions for the establishment, maintenance, and differentiation of murine trophoblast organoids. Murine trophoblast organoids under the maintenance condition contain stem cell-like population...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: Qian Mao Qinying Ye Yiwen Xu Jingwei Jiang Yunhao Fan Lili Zhuang Guohui Liu Tengfei Wang Zhenwu Zhang Teng Feng Shuangbo Kong Jinhua Lu Hui Zhang Haopeng Wang Chao-Po Lin Source Type: research
Chromatin state transitions in the Drosophila intestinal lineage identify principles of cell-type specification
Dev Cell. 2023 Nov 30:S1534-5807(23)00581-6. doi: 10.1016/j.devcel.2023.11.005. Online ahead of print.ABSTRACTTissue homeostasis relies on rewiring of stem cell transcriptional programs into those of differentiated cells. Here, we investigate changes in chromatin occurring in a bipotent adult stem cells. Combining mapping of chromatin-associated factors with statistical modeling, we identify genome-wide transitions during differentiation in the adult Drosophila intestinal stem cell (ISC) lineage. Active, stem-cell-enriched genes transition to a repressive heterochromatin protein-1-enriched state more prominently in enteroe...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: Manon Josserand Natalia Rubanova Marine Stefanutti Spyridon Roumeliotis Marion Espenel Owen J Marshall Nicolas Servant Louis Gervais Allison J Bardin Source Type: research
A reversible epigenetic memory of inflammatory injury controls lineage plasticity and tumor initiation in the mouse pancreas
Dev Cell. 2023 Nov 30:S1534-5807(23)00583-X. doi: 10.1016/j.devcel.2023.11.008. Online ahead of print.ABSTRACTInflammation is essential to the disruption of tissue homeostasis and can destabilize the identity of lineage-committed epithelial cells. Here, we employ lineage-traced mouse models, single-cell transcriptomic and chromatin analyses, and CUT&TAG to identify an epigenetic memory of inflammatory injury in the pancreatic acinar cell compartment. Despite resolution of pancreatitis, our data show that acinar cells fail to return to their molecular baseline, with retention of elevated chromatin accessibility and H3K4...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: David J Falvo Adrien Grimont Paul Zumbo William B Fall Julie L Yang Alexa Osterhoudt Grace Pan Andre F Rendeiro Yinuo Meng John E Wilkinson Friederike D ündar Olivier Elemento Rhonda K Yantiss Erika Hissong Richard Koche Doron Betel Rohit Chandwani Source Type: research
CRIPTO-based micro-heterogeneity of mouse muscle satellite cells enables adaptive response to regenerative microenvironment
Dev Cell. 2023 Nov 30:S1534-5807(23)00585-3. doi: 10.1016/j.devcel.2023.11.009. Online ahead of print.ABSTRACTSkeletal muscle repair relies on heterogeneous populations of satellite cells (SCs). The mechanisms that regulate SC homeostasis and state transition during activation are currently unknown. Here, we investigated the emerging role of non-genetic micro-heterogeneity, i.e., intrinsic cell-to-cell variability of a population, in this process. We demonstrate that micro-heterogeneity of the membrane protein CRIPTO in mouse-activated SCs (ASCs) identifies metastable cell states that allow a rapid response of the populati...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: Ombretta Guardiola Francescopaolo Iavarone Chiara Nicoletti Maurizio Ventre Cristina Rodr íguez Laura Pisapia Gennaro Andolfi Valentina Saccone Eduardo J Patriarca Pier Lorenzo Puri Gabriella Minchiotti Source Type: research
An Arabidopsis Rab18 GTPase promotes autophagy by tethering ATG18a to the ER in response to nutrient starvation
This study finds that ATG18a acts as a downstream effector of RABC1, a member of the poorly characterized Rab18/RabC GTPase subclass in plants. Active RABC1 interacts with ATG18a on the ER, particularly under nutrient starvation. In rabc1 mutants, autophagy is compromised, especially under nutrient deprivation, affecting the ER association and expansion of ATG18a-positive autophagosomes. Furthermore, both dominant-negative and constitutively active RABC1 forms inhibit autophagy. The dominant inactive RABC1 impedes the ER association of ATG18a, whereas the constitutively active RABC1 delays ATG18a detachment from the ER. Co...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: Jiaqi Sun Yang Shao Songyang Wang Xunzheng Li Shuqing Feng Weina Wang Pierre Leroy Chengyang Li Huanquan Zheng Source Type: research
Murine trophoblast organoids as a model for trophoblast development and CRISPR-Cas9 screening
Dev Cell. 2023 Nov 24:S1534-5807(23)00584-1. doi: 10.1016/j.devcel.2023.11.007. Online ahead of print.ABSTRACTThe placenta becomes one of the most diversified organs during placental mammal radiation. The main in vitro model for studying mouse trophoblast development is the 2D differentiation model of trophoblast stem cells, which is highly skewed to certain lineages and thus hampers systematic screens. Here, we established culture conditions for the establishment, maintenance, and differentiation of murine trophoblast organoids. Murine trophoblast organoids under the maintenance condition contain stem cell-like population...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: Qian Mao Qinying Ye Yiwen Xu Jingwei Jiang Yunhao Fan Lili Zhuang Guohui Liu Tengfei Wang Zhenwu Zhang Teng Feng Shuangbo Kong Jinhua Lu Hui Zhang Haopeng Wang Chao-Po Lin Source Type: research
Chromatin state transitions in the Drosophila intestinal lineage identify principles of cell-type specification
Dev Cell. 2023 Nov 30:S1534-5807(23)00581-6. doi: 10.1016/j.devcel.2023.11.005. Online ahead of print.ABSTRACTTissue homeostasis relies on rewiring of stem cell transcriptional programs into those of differentiated cells. Here, we investigate changes in chromatin occurring in a bipotent adult stem cells. Combining mapping of chromatin-associated factors with statistical modeling, we identify genome-wide transitions during differentiation in the adult Drosophila intestinal stem cell (ISC) lineage. Active, stem-cell-enriched genes transition to a repressive heterochromatin protein-1-enriched state more prominently in enteroe...
Source: Developmental Cell - December 6, 2023 Category: Cytology Authors: Manon Josserand Natalia Rubanova Marine Stefanutti Spyridon Roumeliotis Marion Espenel Owen J Marshall Nicolas Servant Louis Gervais Allison J Bardin Source Type: research
