PUF partner interactions at a conserved interface shape the RNA-binding landscape and cell fate in Caenorhabditis elegans
Dev Cell. 2024 Jan 25:S1534-5807(24)00005-4. doi: 10.1016/j.devcel.2024.01.005. Online ahead of print.ABSTRACTProtein-RNA regulatory networks underpin much of biology. C. elegans FBF-2, a PUF-RNA-binding protein, binds over 1,000 RNAs to govern stem cells and differentiation. FBF-2 interacts with multiple protein partners via a key tyrosine, Y479. Here, we investigate the in vivo significance of partnerships using a Y479A mutant. Occupancy of the Y479A mutant protein increases or decreases at specific sites across the transcriptome, varying with RNAs. Germline development also changes in a specific fashion: Y479A abolishes...
Source: Developmental Cell - January 30, 2024 Category: Cytology Authors: Brian H Carrick Sarah L Crittenden Fan Chen MaryGrace Linsley Jennifer Woodworth Peggy Kroll-Conner Ahlan S Ferdous S ündüz Keleş Marvin Wickens Judith Kimble Source Type: research
Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells
Dev Cell. 2024 Jan 22:S1534-5807(24)00002-9. doi: 10.1016/j.devcel.2024.01.002. Online ahead of print.ABSTRACTMutations or dysregulation of nucleoporins (Nups) are strongly associated with neural developmental diseases, yet the underlying mechanisms remain poorly understood. Here, we show that depletion of Nup Seh1 in radial glial progenitors results in defective neural progenitor proliferation and differentiation that ultimately manifests in impaired neurogenesis and microcephaly. This loss of stem cell proliferation is not associated with defects in the nucleocytoplasmic transport. Rather, transcriptome analysis showed t...
Source: Developmental Cell - January 25, 2024 Category: Cytology Authors: Wenxiu Dai Zhixiong Liu Minbiao Yan Ximing Nian Fan Hong Zhihao Zhou Chaomeng Wang Xing Fu Xuewen Li Mengyun Jiang Yanqin Zhu Qiuying Huang Xiaoyun Lu Lichao Hou Ning Yan Qin Wang Jin Hu Wei Mo Xueqin Zhang Liang Zhang Source Type: research
A single fluorescent probe to examine the dynamics of mitochondria-lysosome interplay and extracellular vesicle role in ferroptosis
In this study, we aimed to understand the interplay between mitochondria (Mito) and lysosomes (Lyso) in cell homeostasis and ferroptosis. For this purpose, we designed a single fluorescent probe that marks GSH in Mito and hypochlorous acid (HOCl) in Lyso with two distinct emissions. Using this dual-targeted single fluorescent probe (9-morphorino pyronine), we detected Mito-Lyso interplay in ferroptosis. We disclosed differences in Mito-Lyso interplay depending on the induction of ferroptosis. Although erastin treatment decreased GSH, RSL3 triggered a HOCl burst, and FIN56- and FINO2-induced ferroptosis increased GSH and HO...
Source: Developmental Cell - January 25, 2024 Category: Cytology Authors: Ling-Li Wang Yu-Zhuo Mai Ming-Hua Zheng Guang-Hai Yan Jing-Yi Jin Source Type: research
Blood and bones: Mechanical cues and Hippo signaling drive vascular invasion during limb formation
Dev Cell. 2024 Jan 22;59(2):173-174. doi: 10.1016/j.devcel.2023.12.008.ABSTRACTAlthough mechanical cues are known to influence the postnatal skeleton, the impact of bone cell mechano-transduction on early skeletal development remains less clear. In this issue of Developmental Cell, Collins et al. (2023) report that YAP/TAZ deletion in osteoblast precursors reduces Cxcl12 expression, leading to defects in bone vascularization.PMID:38262346 | DOI:10.1016/j.devcel.2023.12.008 (Source: Developmental Cell)
Source: Developmental Cell - January 23, 2024 Category: Cytology Authors: Marc N Wein Source Type: research
Optimizing rice grain size by attenuating phosphorylation-triggered functional impairment of a chromatin modifier ternary complex
Dev Cell. 2024 Jan 10:S1534-5807(23)00693-7. doi: 10.1016/j.devcel.2023.12.013. Online ahead of print.ABSTRACTHistone acetylation affects numerous cellular processes, such as gene transcription, in both plants and animals. However, the posttranslational modification-participated regulatory networks for crop-yield-related traits are largely unexplored. Here, we characterize a regulatory axis for controlling rice grain size and yield, centered on a potent histone acetyltransferase (chromatin modifier) known as HHC4. HHC4 interacts with and forms a ternary complex with adaptor protein ADA2 and transcription factor bZIP23, whe...
Source: Developmental Cell - January 18, 2024 Category: Cytology Authors: Shao-Yan Shen Ming Ma Chen Bai Wei-Qing Wang Rui-Bin Zhu Qiong Gao Xian-Jun Song Source Type: research
ZFP281 controls transcriptional and epigenetic changes promoting mouse pluripotent state transitions via DNMT3 and TET1
Dev Cell. 2024 Jan 17:S1534-5807(23)00698-6. doi: 10.1016/j.devcel.2023.12.018. Online ahead of print.ABSTRACTThe progression from naive through formative to primed in vitro pluripotent stem cell states recapitulates epiblast development in vivo during the peri-implantation period of mouse embryo development. Activation of the de novo DNA methyltransferases and reorganization of transcriptional and epigenetic landscapes are key events that occur during these pluripotent state transitions. However, the upstream regulators that coordinate these events are relatively underexplored. Here, using Zfp281 knockout mouse and degron...
Source: Developmental Cell - January 18, 2024 Category: Cytology Authors: Xin Huang Sophie Balmer Cong Lyu Yunlong Xiang Vikas Malik Hailin Wang Yu Zhang Bishuang Cai Wei Xie Anna-Katerina Hadjantonakis Hongwei Zhou Jianlong Wang Source Type: research
Acinar-ductal cell rearrangement drives branching morphogenesis of the murine pancreas in an IGF/PI3K-dependent manner
Dev Cell. 2024 Feb 5;59(3):326-338.e5. doi: 10.1016/j.devcel.2023.12.011. Epub 2024 Jan 17.ABSTRACTDuring organ formation, progenitor cells need to acquire different cell identities and organize themselves into distinct structural units. How these processes are coordinated and how tissue architecture(s) is preserved despite the dramatic cell rearrangements occurring in developing organs remain unclear. Here, we identified cellular rearrangements between acinar and ductal progenitors as a mechanism to drive branching morphogenesis in the pancreas while preserving the integrity of the acinar-ductal functional unit. Using ex ...
Source: Developmental Cell - January 18, 2024 Category: Cytology Authors: Jean-Francois Darrigrand Anna Salowka Alejo Torres-Cano Rafael Tapia-Rojo Tong Zhu Sergi Garcia-Manyes Francesca M Spagnoli Source Type: research
Distinct epicardial gene regulatory programs drive development and regeneration of the zebrafish heart
Dev Cell. 2024 Feb 5;59(3):351-367.e6. doi: 10.1016/j.devcel.2023.12.012. Epub 2024 Jan 17.ABSTRACTUnlike the adult mammalian heart, which has limited regenerative capacity, the zebrafish heart fully regenerates following injury. Reactivation of cardiac developmental programs is considered key to successfully regenerating the heart, yet the regulation underlying the response to injury remains elusive. Here, we compared the transcriptome and epigenome of the developing and regenerating zebrafish epicardia. We identified epicardial enhancer elements with specific activity during development or during adult heart regeneration...
Source: Developmental Cell - January 18, 2024 Category: Cytology Authors: Michael Weinberger Filipa C Sim ões Trishalee Gungoosingh Tatjana Sauka-Spengler Paul R Riley Source Type: research
Live-cell imaging defines a threshold in CDK activity at the G2/M transition
Dev Cell. 2024 Jan 15:S1534-5807(23)00695-0. doi: 10.1016/j.devcel.2023.12.014. Online ahead of print.ABSTRACTCyclin-dependent kinase (CDK) determines the temporal ordering of the cell cycle phases. However, despite significant progress in studying regulators of CDK and phosphorylation patterns of CDK substrates at the population level, it remains elusive how CDK regulators coordinately affect CDK activity at the single-cell level and how CDK controls the temporal order of cell cycle events. Here, we elucidate the dynamics of CDK activity in fission yeast and mammalian cells by developing a CDK activity biosensor, Eevee-sp...
Source: Developmental Cell - January 16, 2024 Category: Cytology Authors: Hironori Sugiyama Yuhei Goto Yohei Kondo Damien Coudreuse Kazuhiro Aoki Source Type: research
A mechanical wave travels along a genetic guide to drive the formation of an epithelial furrow during Drosophila gastrulation
Dev Cell. 2024 Feb 5;59(3):400-414.e5. doi: 10.1016/j.devcel.2023.12.016. Epub 2024 Jan 15.ABSTRACTEpithelial furrowing is a fundamental morphogenetic process during gastrulation, neurulation, and body shaping. A furrow often results from a fold that propagates along a line. How fold formation and propagation are controlled and driven is poorly understood. To shed light on this, we study the formation of the cephalic furrow, a fold that runs along the embryo dorsal-ventral axis during Drosophila gastrulation and the developmental role of which is still unknown. We provide evidence of its function and show that epithelial f...
Source: Developmental Cell - January 16, 2024 Category: Cytology Authors: Anna Popkova Ur ška Andrenšek Sophie Pagnotta Primo ž Ziherl Matej Krajnc Matteo Rauzi Source Type: research
Tissue-resident macrophages specifically express Lactotransferrin and Vegfc during ear pinna regeneration in spiny mice
Dev Cell. 2024 Jan 10:S1534-5807(23)00697-4. doi: 10.1016/j.devcel.2023.12.017. Online ahead of print.ABSTRACTThe details of how macrophages control different healing trajectories (regeneration vs. scar formation) remain poorly defined. Spiny mice (Acomys spp.) can regenerate external ear pinnae tissue, whereas lab mice (Mus musculus) form scar tissue in response to an identical injury. Here, we used this dual species system to dissect macrophage phenotypes between healing modes. We identified secreted factors from activated Acomys macrophages that induce a pro-regenerative phenotype in fibroblasts from both species. Trans...
Source: Developmental Cell - January 16, 2024 Category: Cytology Authors: Jennifer Simkin Ajoy Aloysius Mike Adam Fatemeh Safaee Ren ée R Donahue Shishir Biswas Zohaib Lakhani John C Gensel David Thybert Steven Potter Ashley W Seifert Source Type: research
PCGF6 controls murine Tuft cell differentiation via H3K9me2 modification independently of Polycomb repression
We report that PCGF6 regulates mouse intestinal Tuft cell differentiation independently of H2AK119ub1 deposition. We show that PCGF6 chromatin occupancy expands outside Polycomb repressive domains, associating with unique promoter and distal regulatory elements. This occurs in the absence of RING1A/B and involves MGA-mediated E-BOX recognition and specific H3K9me2 promoter deposition. PCGF6 inactivation induces an epithelial autonomous accumulation of Tuft cells that was not phenocopied by RING1A/B loss. This involves direct PCGF6 association with a Tuft cell differentiation program that identified Polycomb-independent pro...
Source: Developmental Cell - January 16, 2024 Category: Cytology Authors: Annachiara Del Vecchio Patrizia Mul é Daniel Fern ández-Pérez Simona Amato Georgia Lattanzi Marika Zanotti Samantha Rustichelli Silvia Pivetti Paola Oldani Andrea Mariani Fabiola Iommazzo Haruhiko Koseki Federica Facciotti Simone Tamburri Karin J Ferra Source Type: research
Disruption of H3K36 methylation provokes cellular plasticity to drive aberrant glandular formation and squamous carcinogenesis
Dev Cell. 2024 Jan 22;59(2):187-198.e7. doi: 10.1016/j.devcel.2023.12.007. Epub 2024 Jan 9.ABSTRACTChromatin organization is essential for maintaining cell-fate trajectories and developmental programs. Here, we find that disruption of H3K36 methylation dramatically impairs normal epithelial differentiation and development, which promotes increased cellular plasticity and enrichment of alternative cell fates. Specifically, we observe a striking increase in the aberrant generation of excessive epithelial glandular tissues, including hypertrophic salivary, sebaceous, and meibomian glands, as well as enhanced squamous tumorige...
Source: Developmental Cell - January 10, 2024 Category: Cytology Authors: Eun Kyung Ko Amy Anderson Carina D'souza Jonathan Zou Sijia Huang Sohyun Cho Faizan Alawi Stephen Prouty Vivian Lee Sora Yoon Keegan Krick Yoko Horiuchi Kai Ge John T Seykora Brian C Capell Source Type: research
Understanding the cell fate and behavior of progenitors at the origin of the mouse cardiac mitral valve
Dev Cell. 2024 Feb 5;59(3):339-350.e4. doi: 10.1016/j.devcel.2023.12.006. Epub 2024 Jan 9.ABSTRACTCongenital heart malformations include mitral valve defects, which remain largely unexplained. During embryogenesis, a restricted population of endocardial cells within the atrioventricular canal undergoes an endothelial-to-mesenchymal transition to give rise to mitral valvular cells. However, the identity and fate decisions of these progenitors as well as the behavior and distribution of their derivatives in valve leaflets remain unknown. We used single-cell RNA sequencing (scRNA-seq) of genetically labeled endocardial cells ...
Source: Developmental Cell - January 10, 2024 Category: Cytology Authors: Batoul Farhat Ignacio Bordeu Bernd Jagla St éphanie Ibrahim Sonia Stefanovic Hugo Blanc Karine Loulier Benjamin D Simons Emmanuel Beaurepaire Jean Livet Michel Puc éat Source Type: research
Dynamic palmitoylation of STX11 controls injury-induced fatty acid uptake to promote muscle regeneration
Dev Cell. 2024 Feb 5;59(3):384-399.e5. doi: 10.1016/j.devcel.2023.12.005. Epub 2024 Jan 9.ABSTRACTDifferent types of cells uptake fatty acids in response to different stimuli or physiological conditions; however, little is known about context-specific regulation of fatty acid uptake. Here, we show that muscle injury induces fatty acid uptake in muscle stem cells (MuSCs) to promote their proliferation and muscle regeneration. In humans and mice, fatty acids are mobilized after muscle injury. Through CD36, fatty acids function as both fuels and growth signals to promote MuSC proliferation. Mechanistically, injury triggers th...
Source: Developmental Cell - January 10, 2024 Category: Cytology Authors: Juan Wang Dong-Lin Li Lang-Fan Zheng Su Ren Zi-Qin Huang Ying Tao Ziyu Liu Yanxia Shang Dejian Pang Huiling Guo Taoling Zeng Hong-Rui Wang He Huang Xingrong Du Haobin Ye Hai-Meng Zhou Peng Li Tong-Jin Zhao Source Type: research