Novel DRAQ5 ™/SYTOX® Blue Based Flow Cytometric Strategy to Identify and Characterize Stem Cells in Human Breast Milk
ConclusionsHence, we developed a novel and reliable flow cytometry based ‐approach to quantify subpopulation of cells in human breast milk with a high content of milk fat globules, lipid droplets, and particles. This approach will improve the identification and quantification of breast milk cells and allow standardizing the flow cytometry‐based evaluation of the stem cell content. © 2018 International Clinical Cytometry Society (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - November 26, 2018 Category: Molecular Biology Authors: Titus Keller, Leonie Wengenroth, Denise Smorra, Kristina Probst, Leo Kurian, Angela Kribs, Bent Brachvogel Tags: Original Article Source Type: research

CD123 expression levels in 846 acute leukemia patients based on standardized immunophenotyping
ConclusionsAuthors evaluated CD123 expression in a large cohort of acute leukemia patients, based on standardized and reproducible methodology. Our results may facilitate stratification of patients most likely to respond to CD123 targeted therapies and serve as reference for CD123 expression (in health and disease). © 2018 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - November 18, 2018 Category: Molecular Biology Authors: Anne E. Bras, Valerie Haas, Arthur Stigt, Mojca Jongen ‐Lavrencic, H. Berna Beverloo, Jeroen G. Marvelde, C. Michel Zwaan, Jacques J.M. Dongen, Jeanette H.W. Leusen, Vincent H.J. Velden Tags: Original Article Source Type: research

Alteration of T Cell Phenotypes in HIV ‐Neurotuberculosis Coinfection
ConclusionsIncreased activation compared to HIV+TB ‐, HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ groups and increased senescence compared to HIV‐nTB+ and HIV‐TB‐ groups were observed in CD8 T cells in HIV+nTB+, suggesting that the frequencies of these T cell subsets are altered to a greater extent in these individuals. © 2018 International Cli nical Cytometry Society (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - November 18, 2018 Category: Molecular Biology Authors: Deepashri Rao, Manjunatha M. Venkataswamy, Ravi Vasanthapuram, P. Satishchandra, Anita Desai Tags: Original Article Source Type: research

Adult Bone Marrow Three ‐Dimensional Phenotypic Landscape of B‐Cell Differentiation
ConclusionWe provide the first analysis of 3D B ‐cell differentiation by multicolor flow cytometry leading to propose reference values for each bone marrow and blood B‐cell compartment. This warrants further exploration of normal and pathological human B‐cell maturation. © 2018 International Clinical Cytometry Society (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - November 18, 2018 Category: Molecular Biology Authors: Claire Carrion, Estelle Gu érin, Nathalie Gachard, Alexandre Guyader, Stéphane Giraut, Jean Feuillard Tags: Original Article Source Type: research

Identification of functionally primitive and immunophenotypically distinct subpopulations in secondary acute myeloid leukemia by mass cytometry
ConclusionsThe identification of this cellular subpopulation highlights the limitations of manual gating in sAML. Our study demonstrates the potential for mass cytometry to elucidate rare subpopulations in highly heterogeneous tumors by utilizing unsupervised high dimensional analysis. © 2018 International Clinical Cytometry Society (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - November 13, 2018 Category: Molecular Biology Authors: Shovik Bandyopadhyay, Jared S. Fowles, Liyang Yu, Daniel A.C. Fisher, Stephen T. Oh Tags: Original Article Source Type: research

BrdU incorporation in multiparameter flow cytometry: A new cell cycle assessment approach in multiple myeloma
ConclusionsCombination of flow cytometry with BrdU or ki67/DAPI staining could become a standard for the determination of MM cell proliferation. Furthermore, in the context of new effective myeloma treatment options, assessment of MM cell proliferation may be valuable, in clinical trials, to identify novel agents that could significantly affect the small proliferative compartment of MM cells. © 2018 International Clinical Cytometry Society (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - November 12, 2018 Category: Molecular Biology Authors: Guilhem Requirand, Nicolas Robert, St éphanie Boireau, Laure Vincent, Anja Seckinger, Salaheddine Bouhya, Patrice Ceballos, Guillaume Cartron, Dirk Hose, Bernard Klein, Jérôme Moreaux Tags: Original Article Source Type: research

Pattern associated leukemia immunophenotypes and measurable disease detection in acute myeloid leukemia or myelodysplastic syndrome with mutated NPM1
ConclusionThe characteristic pattern of LAIPs of myeloid blasts with mutatedNPM1 is common and stable, and allows sensitive and specific detection of AML or MDS with mutatedNPM1 after therapy. © 2018 International Clinical Cytometry Society (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - November 12, 2018 Category: Molecular Biology Authors: Yi Zhou, Andres Moon, Eric Hoyle, Jonathan R. Fromm, Xueyan Chen, Lori Soma, Stephen J. Salipante, Brent L. Wood, David Wu Tags: Original Article Source Type: research

A reproducible strategy for analysis of minimal residual disease measured by Standardized multiparametric flow cytometry in b acute lymphoblastic leukemia
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 24, 2018 Category: Molecular Biology Authors: Hector Sarmiento Palao, Fabi án Tarín, Fernanda Martirena, Eva Barragán, Esperanza Such, Amparo Sempere, Maria Tasso, Pablo Manresa, Francisco López Tags: Letter to the Editor Source Type: research

A reproducible strategy for analisis of minimal residual disease measured by Standarized multiparametric flow cytometry in b acute lymphoblastic leukemia
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 24, 2018 Category: Molecular Biology Authors: Hector Sarmiento Palao, Fabi án Tarín, Fernanda Martirena, Eva Barragán, Esperanza Such, Amparo Sempere, Maria Tasso, Pablo Manresa, Francisco López Source Type: research

Re: Blaha J et al.: The monoclonal anti ‐CD157 antibody clone SY11B5, used for high sensitivity detection of PNH clones on WBCs, fails to detect a common polymorphic variant encoded by BST‐1
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 24, 2018 Category: Molecular Biology Authors: D. Robert Sutherland, Rumina Musani Source Type: research

Interlaboratory comparison of the TransFix ®/EDTA Vacuum Blood Collection tube with the 5 mL Cyto‐Chex® BCT
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 24, 2018 Category: Molecular Biology Authors: Daniel Harrison, Rosalie Ward, Sarah Bastow, Andrew Parr, Susan Macro, Paul K. Wallace Source Type: research

Determination of reference values of MDR ‐ABC transporter activities in CD3+ lymphocytes of healthy volunteers using a flow cytometry based method
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 24, 2018 Category: Molecular Biology Authors: P éter Szerémy, Katalin Tauberné Jakab, Sándor Baráth, András Apjok, Kata Filkor, Zsolt Holló, János Márki‐Zay, János Kappelmayer, Sándor Sipka, Péter Krajcsi, Gergely Toldi Source Type: research

Use of flow cytometry in the phenotypic diagnosis of hodgkin's lymphoma
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 23, 2018 Category: Molecular Biology Authors: Ravnit ‐Kaur Grewal, Manogari Chetty, Emmanuel‐Akinola Abayomi, Ciprian Tomuleasa, Jonathan R. Fromm Source Type: research

Flow Cytometric Analysis of Mechanically Disaggregated Bone Marrow Trephine Biopsies
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 23, 2018 Category: Molecular Biology Authors: M. Wheeler, G. White, S. Brockie, M. Dickson, R. Weinkove Source Type: research

Immunophenotypic Profile of CD34+ Subpopulations and Their Role in the Diagnosis and Prognosis of Patients with De ‐Novo, Particularly Low‐Grade Myelodysplastic Syndromes
Cytometry Part B: Clinical Cytometry, EarlyView. (Source: Cytometry Part B: Clinical Cytometry)
Source: Cytometry Part B: Clinical Cytometry - October 18, 2018 Category: Molecular Biology Authors: Nikolaos Gardikas, Myrofora Vikentiou, Evgenia Konsta, Christos K. Kontos, Sotirios G. Papageorgiou, Aris Spathis, Efthimia Bazani, Anthi Bouchla, Violetta Kapsimali, Katherina Psarra, Periklis Foukas, George Dimitriadis, Vasiliki Pappa Source Type: research