Prof. Luis Diaz and the Early Days of Solid State NMR in Argentina - Recollections from his First Graduate Student.
PMID: 27398421 [PubMed] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 12, 2016 Category: Biotechnology Authors: Frydman L Tags: Curr Pharm Biotechnol Source Type: research

Memory Enhancing Effect of Black Pepper in the AlCl3-Induced Neurotoxicity Mouse Model is Mediated Through its Active Component Chavicine.
CONCLUSION: The study revealed that BP possesses therapeutic effectiveness against neurodegeneration and suggesting Chavicine as a lead compound in disease modification. PMID: 27396401 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 9, 2016 Category: Biotechnology Authors: Ahmed T, Iqbal G, Iqbal A, Mahboob A, Farhat SM Tags: Curr Pharm Biotechnol Source Type: research

Plasma Rich in Growth Factors Inhibits Ultraviolet B Induced Photoageing of the Skin in Human Dermal Fibroblast Culture.
CONCLUSION: This preliminary in vitro study suggests that PRGF is able to prevent UVB derived photo-oxidative stress and to diminish the cell damage caused by ultraviolet irradiation. PMID: 27396402 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 9, 2016 Category: Biotechnology Authors: Anitua E, Pino A, Orive G Tags: Curr Pharm Biotechnol Source Type: research

Reverse Pharmacognosy and Reverse Pharmacology; Two Closely Related Approaches for Drug Discovery Development.
Abstract Pharmacognosy is a science of study of natural products as a source of new drug leads and effective drug development. Rational and economic search for novel lead structures could maximize the speed of drug discovery by using powerful high technology methods. Reverse pharmacognosy is complementary to pharmacognosy which coupled the high throughput screening (HTS), virtual screening and knowledge databases along with the knowledge from traditional medicines which caused identification of numerous in vitro active and selective hits enhancing the speed of drug discovery from natural sources. Besides, reverse ...
Source: Current Pharmaceutical Biotechnology - July 9, 2016 Category: Biotechnology Authors: Saeidnia S, Manayi A, Gohari AR Tags: Curr Pharm Biotechnol Source Type: research

A Systematic Review of the Uterine Relaxant Effect of Herbal Sources.
CONCLUSION: Laboring uterus response to dissimilar tocolytics differs from that of non-laboring uterus. In order to find a treatment for preterm labor, future studies should focus on the laboring uterus and also determine the structure activity relationship of the different tocolytics. This systematic review was registered to the PROSPERO with code number CRD42015027551. PMID: 27396394 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - June 30, 2016 Category: Biotechnology Authors: Rezaeizadeh G, Hantoushzadeh S, Ghiasi S, Nikfar S, Abdollahi M Tags: Curr Pharm Biotechnol Source Type: research

Erratum.
Abstract Corrigendum to Manuscript "Pfeifer T, Buchebner M, Chandak PG, Patankar J, Kratzer A, Obrowsky S, Rechberger GN, Kadam RS, Kompella UB, Kostner GM, Kratky D, Levak-Frank S. Synthetic LXR agonist suppresses endogenous cholesterol biosynthesis and efficiently lowers plasma cholesterol. Curr Pharm Biotechnol. 2011 Feb 1;12(2):285-92." LC-MS internal standard peak areas used in the calculation of brain DMHCA levels in the original manuscript were in error. Brain tissue levels were recalculated using valid internal standard peak areas and the revised Figure 6C is presented below. Thus, brain DMHCA co...
Source: Current Pharmaceutical Biotechnology - June 23, 2016 Category: Biotechnology Authors: Pfeifer T, Buchebner M, Chandak PG, Patankar J, Kratzer A, Obrowsky S, Rechberger GN, Kadam RS, Kompella UB, Kostner GM, Kratky D, Levak-Frank S. Tags: Curr Pharm Biotechnol Source Type: research

Editorial (Thematic Issue: Special Issue for Current Pharmaceutical Biotechnology Miniaturized Platforms & Methods for Pharmaceutical Studies).
Editorial (Thematic Issue: Special Issue for Current Pharmaceutical Biotechnology Miniaturized Platforms & Methods for Pharmaceutical Studies). Curr Pharm Biotechnol. 2016;17(9):753-4 Authors: Li XJ PMID: 27324984 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - June 23, 2016 Category: Biotechnology Authors: Li XJ Tags: Curr Pharm Biotechnol Source Type: research

Antihyperlipidemic, antioxidant and cytotoxic activities of methanolic and aqueous extracts of different parts of star fruit.
This study aimed to evaluate the influence of aqueous and methanolic extracts of ripe and unripe fruits, leaves and stem on the antihyperlipidemic, antioxidant potentials and cytotoxicity of A. carambola. METHODS: Antihyperlipidemic activity was assessed on poloxamer-407 (P-407) induced acute hyperlipidemic rat's model. The potential of antioxidants was assessed in vitro using 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging, 1-diphenyl-2-dipicrylhydrazyl radical scavenging (DPPH) and ferric reducing antioxidant power (FRAP) assays. In addition, cytotoxicity of A. carambola extracts w...
Source: Current Pharmaceutical Biotechnology - June 2, 2016 Category: Biotechnology Authors: Saghir SA, Sadikun A, Al-Suede FS, Abdul Majid AM, Murugaiyah V Tags: Curr Pharm Biotechnol Source Type: research

Targeting cancer with epi-drugs: a precision medicine perspective.
Abstract Recent pan-cancer studies have shown the importance of coupling DNA methylation patterns with transcriptome profiles to reveal tumor subgroups with clinically relevant distinct characteristics. While the coupling patterns remain in most cases matter for further study and/or interpretation, it is emerging that all associations between epigenetic changes and specific cancer histotypes can facilitate the development of novel epidrugs. In particular, together with chemotherapy and chemoprevention of cancer, these epidrugs will target specific enzymes involved in the complex regulation of gene expression. This...
Source: Current Pharmaceutical Biotechnology - May 27, 2016 Category: Biotechnology Authors: Gherardini L, Sharma A, Capobianco E, Cinti C Tags: Curr Pharm Biotechnol Source Type: research

Editorial: Lipidic Nano-carrier Delivery of "Big" Molecules.
Editorial: Lipidic Nano-carrier Delivery of "Big" Molecules. Curr Pharm Biotechnol. 2016;17(8):661 Authors: Elbayoumi T PMID: 27215370 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - May 25, 2016 Category: Biotechnology Authors: Elbayoumi T Tags: Curr Pharm Biotechnol Source Type: research

Lipid Nanoparticles to Deliver miRNA in Cancer.
Abstract MicroRNAs (miRNAs) are a class of post-transcriptional gene expression modulators. In the past two decades, over 1500 human miRNAs were discovered. These small non-coding RNAs regulate various biological processes, including cell growth, proliferation, differentiation, and cell death. Thus, miRNAs have been proposed as new therapeutical agents in different multifactorial diseases such as cancer. Since miRNAs therapies represent a great promise, many research studies have been focused on the development of delivery strategies to overcome miRNAs biopharmaceutical issues. Lipid delivery systems are undoubted...
Source: Current Pharmaceutical Biotechnology - May 25, 2016 Category: Biotechnology Authors: Campani V, De Rosa G, Misso G, Zarone MR, Grimaldi A Tags: Curr Pharm Biotechnol Source Type: research

Systematic evaluation of drug-loaded hydrogels for application in osteosarcoma treatment.
Abstract This is a literature review of studies focusing on the preparation of hydrogels for use as oncological drug delivery systems in the treatment of osteosarcoma (OS).The databases of the US National Library of Medicine National Institutes of Health, Embase, OVID, and Cochrane Library, and the references of retrieved studies, were traced from 1843 to December 21, 2015, without language restrictions. The obtained data were evaluated by complementary statistical methods. Potentially relevant studies were found and included in the analysis. OS-specific chemotherapeutic agents can be successfully embedded within ...
Source: Current Pharmaceutical Biotechnology - May 19, 2016 Category: Biotechnology Authors: Gumustas SA, Isyar M, Topuk S, Yilmaz I, Oznam K, Onay T, Ofluoglu O, Mahirogullari M Tags: Curr Pharm Biotechnol Source Type: research

H2020 and beyond: skip discrepancy between theory and practice of Personalized Medicine. A position paper by the Italian Society of Personalized Medicine.
Abstract Many unsolved practical issues, from technical and scientific to ethical, legal and economic topics, are slowing down the translation of Personalized Medicine principles into medical practice. The Italian Society of Personalized Medicine exposes here its point of view, based on the real-world practice of precision medicine carried-out in Italian healthcare structures. PMID: 27194357 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - May 19, 2016 Category: Biotechnology Authors: Borro M, Simmaco M, Aceti A, Barni S, De Luca A, Fineschi V, Frati P, Girardi P, Miozzo M, Nati G, Nicoletti F, Santini D, Marchetti P Tags: Curr Pharm Biotechnol Source Type: research

Microfluidic Devices for Automation of Assays on Drosophila Melanogaster for Applications in Drug Discovery and Biological Studies.
Abstract Drug discovery is a long and expensive process, which usually takes 12-15 years and could cost up to ~$1 billion. Conventional drug discovery process starts with high throughput screening and selection of drug candidates that bind to specific target associated with a disease condition. However, this process does not consider whether the chosen candidate is optimal not only for binding but also for ease of administration, distribution in the body, effect of metabolism and associated toxicity if any. A holistic approach, using model organisms early in the drug discovery process to select drug candidates tha...
Source: Current Pharmaceutical Biotechnology - May 19, 2016 Category: Biotechnology Authors: Ghaemi R, Ravi Selvaganapathy P Tags: Curr Pharm Biotechnol Source Type: research

Effect of thermal and shear stressors on the physical properties, structural integrity and biological activity of the anti-TNF-alpha monoclonal antibody, Infliximab.
Abstract The influence of thermal and shear stressors on the stability of the anti-TNF-α monoclonal antibody (mAb), Infliximab® (INF) was investigated. INF at concentrations of 1, 4 and 10 mg/ml was subjected to thermal stress at temperatures of 25-65°C and to shear force by sonication for 1 and 3 minutes. The stressed samples were analysed for physical properties by particle size, zeta potential, for structural integrity by gel electrophoresis (SDS-PAGE) and circular dichroism, INF content by UV spectroscopy and for biological activity by ELISA. Results show no change in physical properties or struc...
Source: Current Pharmaceutical Biotechnology - May 19, 2016 Category: Biotechnology Authors: Alsaddique JA, Pabari RM, Ramtoola Z Tags: Curr Pharm Biotechnol Source Type: research

Recent developments of three-dimensional paper-based electrochemical devices for cancer cell detection and anticancer drug screening.
Abstract Cancer, as the most serious threat to human health, poses millions of deaths in the world each year. In vitro cancer cell and biomarker detection is of great importance for early cancer diagnosis and therapy, which needs highly sensitive, selective and quantitative diagnosis platforms. Three-dimensional paper-based electrochemical devices (3D-PEDs) have attracted considerable interest in biomedical fields in recent years based on their simplicity, low cost, portability, high sensitivity and selectivity. Recently, since 3D-PEDs can particularly provide in vitro 3D structures to mimic the native 3D cell mic...
Source: Current Pharmaceutical Biotechnology - May 19, 2016 Category: Biotechnology Authors: Li Z, Hu J, Xu F, Li F Tags: Curr Pharm Biotechnol Source Type: research

Empowering translational research in fetal growth restriction: Sheep and swine animal models.
The objective of the current review is to offer an overview on the possibilities of large animals for translational research in IUGR related to inadequate maternal conditions and/or placental dysfunction. PMID: 27194361 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - May 19, 2016 Category: Biotechnology Authors: Gonzalez-Bulnes A, Astiz S, Parraguez VH, Garcia-Contreras C, Vazquez-Gomez M Tags: Curr Pharm Biotechnol Source Type: research

Editorial: Metabolic Syndrome in Pregnancy.
PMID: 27165110 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - May 16, 2016 Category: Biotechnology Authors: Kimber-Trojnar Ż Tags: Curr Pharm Biotechnol Source Type: research

Characterization and Antihypertensive Effect of the Complex of ( )-β-pinene in β-cyclodextrin.
In conclusion, the slurry complexation method was the best among them. Pharmacological results demonstrated that the complex promoted antihypertensive effect. Furthermore, βP induced endothelium-independent vasorelaxation possibly caused by the inhibition of the Ca2+ influx through L-type Ca2+ channel associated to a decrease in calcium sensitivity. PMID: 27109904 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - April 25, 2016 Category: Biotechnology Authors: Moreira ÍJ, Menezes PP, Serafini MR, Araújo AA, Quintans-Júnior LJ, Bonjardim LR, Filho VJ, Júnior DB, Santos SL, Júnior WL, Scotti L, Scotti MT, Santos MR Tags: Curr Pharm Biotechnol Source Type: research

Fruit as potent natural antioxidants and their biological effects.
Abstract The consumption of fruit has increased in the last 20 years, along with the growing recognition of its nutritional and protective values. Many of the benefits of a diet rich in fruit are attributed to the presence of different bioactive substances, such as vitamins, carotenoids and phenolic compounds. Flavanoids, a class of phenolic compounds, present particular antioxidant activity and thus provide protection against cellular damage caused by reactive oxygen species. Research suggests that an increased intake of plant foods is associated with a reduced incidence of chronic disease. There is currently a g...
Source: Current Pharmaceutical Biotechnology - April 25, 2016 Category: Biotechnology Authors: Gomes-Rochette NF, Da Silveira Vasconcelos M, Nabavi SM, Mota EF, Nunes-Pinheiro DC, Daglia M, De Melo DF Tags: Curr Pharm Biotechnol Source Type: research

A systematic review of the uterine relaxant effect of herbal sources.
CONCLUSION: Laboring uterus response to dissimilar tocolytics differs from that of non-laboring uterus. In order to find a treatment for preterm labor, future studies should focus on the laboring uterus and also determine the structure activity relationship of the different tocolytics. This systematic review was registered to the PROSPERO with code number CRD42015027551. PMID: 27109906 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - April 25, 2016 Category: Biotechnology Authors: Rezaeizadeh G, Hantoushzadeh S, Ghiasi S, Nikfar S, Abdollahi M Tags: Curr Pharm Biotechnol Source Type: research

Lipid Nanoparticles and their Application in Nanomedicine.
Abstract In the recent years many different nanotechnology platforms have been developed for both diagnostics and cancer therapy. Researchers have been attracted to use lipid-based nanoparticles with particle size approximately 100 nm as a new pharmaceutical delivery system or pharmaceutical formulation that can be assembled from different types of lipid or other chemical components in order to overcome biological barriers. This mini-review highlights the recently published studies in the development of lipid-based nanoparticles as appropriate vehicles in cancer diagnosis and therapy with focus on their chemical s...
Source: Current Pharmaceutical Biotechnology - April 15, 2016 Category: Biotechnology Authors: Rajabi M, Mousa SA Tags: Curr Pharm Biotechnol Source Type: research

In vitro Evaluation of Copaifera oblongifolia Oleoresin against Bacteria Causing Oral Infections and assessment of its Cytotoxic Potential.
This study also investigated the bactericidal kinetics (time-kill curves) and the synergistic effect of the C. oblongifolia oleoresin. Additionally, this study evaluated the cytotoxic activity of the oleoresin toward V79 cells by means of the colony-forming assay. The C. oblongifolia oleoresin gave promising MIC and MBC values, which ranged from 25 to 200 μg/mL. Analysis of the MBIC50 values of the oleoresin revealed it displayed biofilm inhibitory activity against all the assayed bacteria. Analysis of the bactericidal kinetics showed different behaviors of the oleoresin against the tested bacteria at the different time...
Source: Current Pharmaceutical Biotechnology - April 15, 2016 Category: Biotechnology Authors: da Silva Moraes T, Leandro LF, de Oliveira Silva L, Santiago MB, Souza AB, Furtado RA, Tavares DC, Veneziani RC, Ambrósio SR, Bastos JK, Martins CH Tags: Curr Pharm Biotechnol Source Type: research

Nanocarriers Conjugated with Cell Penetrating Peptides: New Trojan Horses by Modern Ulysses.
Abstract Nanomedicine has opened the way to the design of more efficient diagnostics and therapeutics. Moreover,recent literature has illustratedthe use of short cationic and/or amphipathic peptides, known as cell-penetrating peptides (CPPs), for mediating advanceddrug delivery.CPPs exploit their ability to enter cells to enhance the uptake of many cargoes ranging from small molecules to proteins. The distinctiveproperties of NC based systems provide unforeseen benefitsover pure drugsfor biomedical applicationsand constitute a challenging research field particularly focused on imaging and delivery; nonetheless, se...
Source: Current Pharmaceutical Biotechnology - April 15, 2016 Category: Biotechnology Authors: Zappavigna S, Misso G, Falanga A, Perillo E, Novellino E, Galdiero M, Grieco P, Caraglia M, Galdiero S Tags: Curr Pharm Biotechnol Source Type: research

Nutraceuticals as Lipid-Lowering Treatment in Pregnancy and Their Effects on the Metabolic Syndrome.
Abstract Maternal nutrition and lifestyle before and during pregnancy influence both mother and offspring's health and can be correlated with the metabolic syndrome in later life. Findings from animal and human studies indicate that nutrition during pregnancy has an important role in microbiological, metabolic, physiologic and immunologic development and homeostasis. A low nutritional intake in early pregnancy may represent a risk for adverse effects during pregnancy as well as on birth outcome. It seems that dietary supplementation with probiotics in perinatal period may represent safe and practical approach in d...
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Al-Dughaishi T, Nikolic D, Al-Hashmi K, Al-Waili K, Rizzo M, Al-Rasadi K Tags: Curr Pharm Biotechnol Source Type: research

Prospects of Nanocarriers for Oral Delivery of Bioactives Using Targeting Strategies.
Abstract The oral route is the preferred mode of administration, however the poor intestinal absorption of many bioactive compounds limit their efficacy. Several strategies have been developed to overcome the low oral bioavailability of bioactive compounds. Nanocarriers present a unique opportunity to overcome this limitation due to their diverse bioactive carriage potential, surface functionality and design flexibility. Despite these favorable characteristics, the oral delivery of nanocarriers faces several challenges which are discussed in this review. The review addresses the different mechanisms of transport a...
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Parayatha NN, Nehoffa H, Taurina S, Greish K Tags: Curr Pharm Biotechnol Source Type: research

Janus Nano- and Microparticles as Smart Drug Delivery Systems.
Abstract Research has been lately prospering in the field of Janus nanoparticles, focusing on new synthesis techniques and their application in various fields. This is due to the unique characteristics possessed by this type of particles such as their anisotropic nature, and their synergistic potential for combination therapy in addition to the multilevel targeting. These unique features are essential in several biomedical applications, especially the controlled drug delivery. Various techniques have been used for the synthesis of Janus nanoparticles including; masking, self-assembly, microfluidic and phase separa...
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: El-Sherbiny IM, Abbas Y Tags: Curr Pharm Biotechnol Source Type: research

Lipid-based siRNA Delivery Systems: Challenges, Promises and Solutions Along the Long Journey.
Abstract RNA interference (RNAi) is an evolutionary conserved highly specific gene-silencing mechanism initiated by small interfering RNA (siRNA) molecules. Fast-paced preclinical and clinical studies helped the siRNA technology become an efficient tool for undruggable targets in different diseases including genetic diseases, viral diseases and cancer. Despite great feature of siRNAs that can down-regulate any protein in the cells, the full potential and the success of the preclinical studies could not be translated into largely successful clinical outcomes. It has become clear that the possibility of overcoming t...
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Sarisozen C, Salzano G, Torchilin VP Tags: Curr Pharm Biotechnol Source Type: research

Nucleic-Acid Delivery Using Lipid Nanocapsules.
In conclusion, LNCs are very good candidates to deliver nucleic acids to cells in the course of anti-cancer therapies. PMID: 27033510 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Lagarce F, Passirani C Tags: Curr Pharm Biotechnol Source Type: research

Characterization of Pharmaceutical IgG and Biosimilars Using Miniaturized Platforms and LC-MS/MS.
Abstract Therapeutic monoclonal antibodies (mAbs) have made a tremendous impact in treating patients with various diseases. MAbs are designed to specifically target a cell and illicit a response from the immune system to destroy the cell. As originator mAb drug patents are coming to an end, generic pharmaceutical companies are poised to replicate and produce so-called biosimilar drugs. MAbs are significantly more complicated than small drugs to analyze and produce. The mAb proteoform and glycoform must be as similar to the original drug as possible to be a viable replacement. The mAb proteoform is well characteriz...
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Wooding KM, Peng W, Mechref Y Tags: Curr Pharm Biotechnol Source Type: research

Biopharmaceutical potential of two Ramalina lichens and their metabolites.
Abstract This paper studies the phytochemical analysis of the acetone extracts of the Ramalina fraxinea and Ramalina fastigiata lichens and the antioxidant, antimicrobial and antitumour activities of these species and their constituents. The phytochemical analysis of two Ramalina species was evaluated using HPLC-UV test. The depsides (evernic acid, obtusatic acid, sekikaic acid and atranorin), depsidones (protocetraric acid) and dibenzofurane (usnic acid) were identified from these lichens. Antioxidant activity was evaluated by DPPH assay, reducing power assay and by measuring the amounts of total phenolics in ext...
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Ristić S, Ranković B, Kosanić M, Stamenković S, Stanojković T, Sovrlić M, Manojlović N Tags: Curr Pharm Biotechnol Source Type: research

Synergistic Combination of Doxorubicin and Paclitaxel Delivered by Blood Brain Barrier and Glioma Cells Dual Targeting Liposomes for Chemotherapy of Brain Glioma.
In conclusion, synergistic combination of doxorubicin and paclitaxel delivered by the TF/TAT-LP could efficiently target to the brain glioma with satisfying treatment efficiency, which may be a promising formulation for glioma therapy. PMID: 27033513 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Chen X, Yuan M, Zhang Q, Yang Y, Gao H, He Q Tags: Curr Pharm Biotechnol Source Type: research

Synthesis of Iron Oxide Micro and Nanoparticles from Aluminum Industry Waste and its application in the decolorization of Reactive Blue 235 dye.
Abstract Iron oxide micro and nanoparticles were successfully synthesized from Red Mud (RM) using a simple two- step process. RM was characterized using various techniques such as X-Ray Diffractometer (XRD), Fourier Transform- Infra Red (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX) analysis and Carbon Hydrogen Nitrogen (CHN) microanalysis. Red mud derived iron oxide micro and nanoparticles (RIMNP) were characterized using techniques such as XRD, FTIR, SEM/EDX, Transmission Electron Microscopy (TEM) and Vibrating Sample Magnetometry (VSM). RIMNP were employed as a Fenton lik...
Source: Current Pharmaceutical Biotechnology - April 1, 2016 Category: Biotechnology Authors: Natarajan E, Ponnaiah GP Tags: Curr Pharm Biotechnol Source Type: research

Editorial: Special Issue in Memory of Prof. Dr. Luis Eduardo Diaz: Current Topics in Pharmaceutical Biotechnology.
PMID: 26956106 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - March 11, 2016 Category: Biotechnology Authors: Desimone MF Tags: Curr Pharm Biotechnol Source Type: research

Healing or Not Healing.
Abstract Healing process might be considered as a byproduct of the mechanisms underlying the biological defense system consisting of hemostasis and clotting, the innate immune system, and fibrogenesis. But there is no biological process that does not potentially entail high costs through trade-offs with other life-history parameters and that might be seen as collateral damage. Depending on the balance among the robust and flexible modular defense system, which will be deployed in many different arrays, the structural outcome of the healing process will not resolve with a unitary outcome. Drawing on the regenerativ...
Source: Current Pharmaceutical Biotechnology - March 11, 2016 Category: Biotechnology Authors: Padilla S, Sanchez M, Padilla I, Orive G, Anitua E Tags: Curr Pharm Biotechnol Source Type: research

Insect Larvae: A New Platform to Produce Commercial Recombinant Proteins.
Abstract In Biotechnology, the expression of recombinant proteins is a constantly growing field and different hosts are used for this purpose. Some valuable proteins cannot be produced using traditional systems. Insects from the order Lepidoptera infected with recombinant baculovirus have appeared as a good choice to express high levels of proteins, especially those with post-translational modifications. Lepidopteran insects, which are extensively distributed in the world, can be used as small protein factories, the new biofactories. Species like Bombyx mori (silkworm) have been analyzed in Asian countries to prod...
Source: Current Pharmaceutical Biotechnology - March 11, 2016 Category: Biotechnology Authors: Targovnik AM, Arregui MB, Bracco LF, Urtasun N, Baieli MF, Segura MM, Simonella MA, Fogar M, Wolman FJ, Cascone O, Miranda MV Tags: Curr Pharm Biotechnol Source Type: research

Innovative Immobilization Matrices.
We present a brief survey of some of the recent work of Professor Luis E. Díaz, performed together with his students and collaborators at the University of Buenos Aires. Dr Luis E. Díaz has been involved in research on biochemical and pharmaceutical sciences solving scientific and industry problems for over 40 years until he passed away. Prof. Díaz scientific interests included various topics from NMR spectroscopy to biomedicine but fundamentally he focused in various aspects of chemistry (analytical, organic, inorganic and environmental). This is not a complete survey but a sampling of prominent proje...
Source: Current Pharmaceutical Biotechnology - March 11, 2016 Category: Biotechnology Authors: Alvarez GS, Echazu MI, Bertinatto JA, Catalano PN, Copello GJ, Foglia ML, Gonzalez JA, Giorgieri SA, Iglesias SL, Mebert AM, Santo-Orihuela PL, Tuttolomondo MV, Villanueva EE, Desimone MF Tags: Curr Pharm Biotechnol Source Type: research

Potential Application of Plant-derived Bioengineered Human VEGF for Tissue Regeneration.
Abstract The design and development of novel biological drugs are among the most exciting new areas of biotechnology which are gaining the attention of scientists. In the last few decades several fabrication processes have been proposed and developed for the production of recombinant growth factors. However, traditional production processes have several limitations in terms of scale- up, cost-efficiency and purity grade of the proteins. In the present study, we propose for the first time the proof-of-concept of large-scale production of growth factors in plants as a new alternative to other production processes. W...
Source: Current Pharmaceutical Biotechnology - March 11, 2016 Category: Biotechnology Authors: Laura W, Pablo L, Arantxa A, Isabel B, Alberto R, Silvia J, Noelia C, de Francisco Ana, Raquel C, Gorka O Tags: Curr Pharm Biotechnol Source Type: research

Nanotoxicological Effects of SiO2 Nanoparticles on Spodoptera frugiperda Sf9 Cells.
In conclusion, we have demonstrated that the NPs possess an effect that is highly influenced by the size, charge and concentration. Although, silica NPs are being used in the biomedical field, these results contribute to further understanding the risk that could be associated to nanoparticles and how these can be modified in order to meet the requirements of each desired application. PMID: 26956111 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - March 11, 2016 Category: Biotechnology Authors: Santo-Orihuela PL, Foglia ML, Targovnik AM, Miranda MV, Desimone MF Tags: Curr Pharm Biotechnol Source Type: research

The Unbiased Search of Biomarkers in Neurodegenerative Diseases.
Abstract A clear link exists between the extension of life expectancy throughout the world and the increased incidence of age-related neurodegenerative disorders. Diseases like Alzheimer's and Parkinson's are chronic diseases with devastating consequences for patients and their families. They also represent a major economic cost for society. Therapeutic progress has been made mainly by alleviating some of the symptoms of these diseases, but currently no cure is available. Attempts to develop new therapies have been hampered mainly because of gaps in our current knowledge about the pathogenic mechanism underlying n...
Source: Current Pharmaceutical Biotechnology - March 11, 2016 Category: Biotechnology Authors: Ferrara P Tags: Curr Pharm Biotechnol Source Type: research

Short term, low dose simvastatin pretreatment alters memory immune function following secondary Staphylococcus aureus infection.
In this study, we report that levels of total serum IgG2c, following primary infection, were decreased in simvastatin pretreated mice, and investigate the effect of simvastatin treatment, prior to primary infection, on immune responses activated during secondary S. aureus infection. A secondary infection model was implemented whereby simvastatin pretreated and control mice were re-infected with S. aureus 14 days after primary infection, with no additional simvastatin treatment, and assessed for survival and alterations in immune function. While survivability to secondary S. aureus infection was not different between simvas...
Source: Current Pharmaceutical Biotechnology - March 1, 2016 Category: Biotechnology Authors: Smelser LK, Walker C, Burns EM, Curry M, Black N, Metzler JA, McDowell SA, Bruns HA Tags: Curr Pharm Biotechnol Source Type: research

Anticoagulant Therapy in Pregnant Patients with Metabolic Syndrome: A Review.
Abstract Pregnancy is a specific state of heightened coagulability related to the increase in procoagulant agents and to the reduced fibrinolysis. Pregnancy is associated with a 4-fold increased risk of developing venous thromboembolism (VTE) and this risk still increases to 14-fold during puerperium. A correlation between the metabolic syndrome and development of cardiovascular events and cerebrovascular incidents has been described. Such a relationship is referred to a hypercoagulable state due to increased serum levels of the plasminogen activator inhibitor-1 (PAI-1), fibrinogen, factor (F) VII and VIII, von Wi...
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Mierzyński R, Poniedziałek-Czajkowska E, Kimber-Trojnar Ż, Leszczyńska-Gorzelak B, Oleszczuk J Tags: Curr Pharm Biotechnol Source Type: research

Plasma Rich in Growth Factors Enhances Wound Healing and Protects from Photo-oxidative Stress in Dermal Fibroblasts and 3D Skin Models.
CONCLUSION: These findings suggest that this autologous approach might be useful for antifibrotic wound healing and provide an effective protection against sun derived photo-oxidative stress regardless the age of the patient. PMID: 26927211 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Anitua E, Pino A, Jaen P, Orive G Tags: Curr Pharm Biotechnol Source Type: research

Nature and Nurture in the Early-Life Origins of Metabolic Syndrome.
Abstract The combination of genetic background together with food excess and lack of exercise has become the cornerstone of metabolic disorders associated to lifestyle. The scenario is furthermore reinforced by their interaction with other environmental factors (stress, sleeping patterns, education, culture, rural versus urban locations, and xenobiotics, among others) inducing epigenetic changes in the exposed individuals. The immediate consequence is the development of further alterations like obesity and metabolic syndrome, and other adverse health conditions (type-2 diabetes, cardiovascular diseases, cancer, re...
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Gonzalez-Bulnes A, Astiz S, Ovilo C, Garcia-Contreras C, Vazquez-Gomez M Tags: Curr Pharm Biotechnol Source Type: research

Microfabricated Devices for Studying the Metabolism and Cytotoxicity of Drug Candidates.
Abstract During drug development, large libraries of new chemical entities (NCEs) are generated and undergo in vitro screens of metabolism and cytotoxicity. These screens are heavily relied upon to select lead compounds with the highest chance of success in pre-clinical studies and clinical trials, but suffer from limited in vivo predictive power despite using human liver-derived components. There is a need for new assays that utilize smaller reagent volumes to reduce the cost of these high-throughput screens; better mimic the liver environment; and ultimately account for toxicities in other major organ systems. M...
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Whitman NA, McIntosh JC, Penley JB, Lockett MR Tags: Curr Pharm Biotechnol Source Type: research

Microfluidic Devices for Circulating Tumor Cells Isolation and Subsequent Analysis.
Abstract The research of circulating tumor cells (CTCs) has drawn much attention in recent years. It is because of the potential values of CTCs in early diagnosis of cancer, management of clinical treatment, exploration of metastatic mechanism, and development of personalized medicine. However, isolating CTCs has been technically challenging due to their rare numbers in blood. Recently, a variety of microfluidic devices have been developed for CTC isolation, and these devices can realize high capture efficiency and high purity. While enumeration of CTCs has been achieved, further cellular and DNA analysis on the c...
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Khamenehfar A, Li PC Tags: Curr Pharm Biotechnol Source Type: research

A prevention of pre-eclampsia with the use of acetylsalicylic acid and low-molecular weight heparin - molecular mechanisms.
Abstract Pre-eclampsia appears to be the main cause for the maternal and fetal morbidity and mortality. Pregnant women with pre-eclampsia are more likely to be threatened with conditions which potentially may be lethal, such as: disseminated intravascular coagulation, cerebral hemorrhage, liver and renal failure. Pregnancy complicated with pre-eclampsia is also associated with a greater risk for iatrogenic prematurity, intrauterine growth retardation, premature abruption of placenta, and even intrauterine fetal death. In the majority of cases the reasons for arterial hypertension among pregnant women remain obscur...
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Darmochwal-Kolarz D, Kolarz B, Korzeniewski M, Kimber-Trojnar Z, Patro-Malysza J, Mierzynski R, Przegalinska-Kałamucka M, Oleszczuk J Tags: Curr Pharm Biotechnol Source Type: research

Tumor necrosis factor-α, a regulator and therapeutic agent on breast cancer.
Tumor necrosis factor-α, a regulator and therapeutic agent on breast cancer. Curr Pharm Biotechnol. 2016 Feb 29; Authors: Liu D Abstract The cell-mediated immunity and cytotoxic agents play a significant role on tumor cell apoptosis. Tumor necrosis factor-α (TNF-α) is an intricate linker between inflammation and cancer through mediating the process of apoptosis and cell-mediated immunity. A variety of evidences have confirmed the critical role of TNF-α on tumor migration, proliferation, matrix degradation, tumor metastasis, invasion, and angiogenesis. Through binding to recepto...
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Liu D Tags: Curr Pharm Biotechnol Source Type: research

Nano-Sized Technologies for Miconazole Skin Delivery.
Abstract Various nano-based strategies for increasing the efficiency of topical drugs have offered the potential advantage of miconazole skin delivery. Miconazole nitrate is an antifungal drug with a drawback of poor skin-penetration in the treatment of deep seated fungal skin infections. Drug entrapment in nanoparticles such as ethosome, liposomes, solid lipid nanoparticles (SLNs) and nano structured lipid carriers (NLCs) can facilitate localized drug delivery and remove the skin barriers for an efficient drug delivery. Different nano-formulations have been recently examined for the controlled release, retention ...
Source: Current Pharmaceutical Biotechnology - February 29, 2016 Category: Biotechnology Authors: Firooz A, Namdar R, Nafisi S, Maibach HI Tags: Curr Pharm Biotechnol Source Type: research

Effect of a Metalloantibiotic Produced by Pseudomonas aeruginosa on Klebsiella pneumoniae Carbapenemase (KPC)-producing K. pneumoniae.
ade G Abstract Multidrug-resistant organisms (MDRO) are a great problem in hospitals, where thousands of people are infected daily, with the occurrence of high mortality rates, especially in infections caused by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-producing Kpn). The challenge is to find new compounds that can control KPC producing-Kpn infections. The aim of this study was to evaluate the antibiotic activity of the F3d fraction produced by the Pseudomonas aeruginosa LV strain against clinical isolates of KPC-producing Kpn. The results showed that the minimum inhibitory concentration of...
Source: Current Pharmaceutical Biotechnology - February 22, 2016 Category: Biotechnology Authors: Kerbauy G, Vivan AC, Simões GC, Simionato AS, Pelisson M, Vespero EC, Costa SF, de J Andrade CG, Barbieri DM, Mello JC, Morey AT, Yamauchi LM, Yamada-Ogatta SF, de Oliveira AG, Andrade G Tags: Curr Pharm Biotechnol Source Type: research