Screening and Identification of ssDNA aptamers against HN protein for detection of bovine parainfluenza virus type 3 antibodies in serum.
Abstract Bovine parainfluenza virus type 3 (BPIV3) is a major but often overlooked pathogen that causes respiratory disease in cattle, especially during transportation and in feedlot situations. In the present study, ssDNA aptamers with high affinity and specificity against the HN protein of BPIV3 were selected using microplates as the matrix. After eleven rounds selection, thirty-four different DNA sequences were obtained in total, wherein w-32, w-33, and w-34 were repeated seven, eleven, and nine times, and with Kd values of 56.57 ± 2.7 nM, 24.64 ± 2.84 nM, and 31.3 ± 3.32 nM, respectively. ...
Source: Current Pharmaceutical Biotechnology - October 31, 2018 Category: Biotechnology Authors: Cheng J, Wang JW, Liu Y, Wu QM, Wang Z Tags: Curr Pharm Biotechnol Source Type: research

The Association of Fractalkine receptor (T280M) Polymorphism in the Pathogenesis of Acute Coronary Syndrome in the Egyptian Population.
CONCLUSIONS: This study shows that CX3CR1 T280M polymorphism does not affect the incidence of ACS the Egyptian population. Moreover, none of the genotypes were associated with higher FKN levels. PMID: 30381070 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 31, 2018 Category: Biotechnology Authors: Hassan DS, Hashad IM, Abdel Rahman MF, Abdel-Maksoud SM Tags: Curr Pharm Biotechnol Source Type: research

Erythropoietin directly affects human macrophage functionality.
Abstract We studied direct effects of erythropoietin (Epo) on functional properties of human monocytes/macrophages (Mc/Mphs) in vitro. Cells expressing CD14 marker (a co-receptor for bacterial LPS) were isolated from human peripheral blood mononuclear cells (PBMCs) by positive magnetic separation. We showed that Epo treatment reduce the percentages of CD14+ cells, CD124 (alpha subunit of IL-4 receptor)+ cells and CD197 (CCR7) + cells in non-activated Mph cultures without affecting the levels of CD16 (low-affinity Fc-receptor)+ and CD119 (interferon-γ (IFN-γ) receptor)+ cells. Epo also markedly reduced ...
Source: Current Pharmaceutical Biotechnology - October 31, 2018 Category: Biotechnology Authors: Melashchenko OV, Meniailo ME, Malashchenko VV, Gazatova ND, Goncharov AG, Seledtsova GV, Seledtsov VI Tags: Curr Pharm Biotechnol Source Type: research

Factors regulating human extravillous trophoblast invasion.
Abstract The invasion of an extravillous trophoblast (EVT) into maternal decidual tissues, especially toward maternal spiral arteries, is an essential process in human placental formation and subsequent normal fetal development. However, the precise regulatory mechanisms to induce EVT invasion toward arteries and/or to protect EVT from further invasion are not well understood. We found that a chemokine receptor, CCR1, was specifically expressed on EVT migrating toward maternal arteries. Using EVT isolated from a primary villous explant culture, RANTES, which is one of the ligands for CCR1, was shown to enhance EVT...
Source: Current Pharmaceutical Biotechnology - October 29, 2018 Category: Biotechnology Authors: Fujiwara H, Matsumoto H, Sato Y, Horie A, Ono M, Nakamura M, Mizumoto Y, Kagami K, Fujiwara T, Hattori A, Maida Y, Daikoku T, Imakawa K, Araki Y Tags: Curr Pharm Biotechnol Source Type: research

Anti-Inflammatory Effects Of Variola Virus Tnf Decoy Receptor In Experimental Model Of Contact Dermatitis.
Abstract Large DNA poxviruses encode a diverse family of secreted proteins that modulate host inflammatory and antiviral responses, in particular by inhibiting one of the key players of the mammalian immune system, the tumor necrosis factor (TNF). We investigated the effects of a recombinant variola (smallpox) virus TNF-decoy receptor (VARV-CrmB) in a murine model of contact dermatitis. Our results demonstrate that the VARV-CrmB protein significantly reduces the 2,4-dinitrochlorbenzene (DNCB)-induced migration of skin leukocytes during the sensitization phase and suppresses ear oedema during the elicitation phase ...
Source: Current Pharmaceutical Biotechnology - October 29, 2018 Category: Biotechnology Authors: Viazovaia EA, Gileva IP, Toporkova LB, Shchelkunov SN, Orlovskaya IA Tags: Curr Pharm Biotechnol Source Type: research

A microplate-based platform with immobilized human glutathione transferase A1-1 for high-throughput inhibitor screening.
Abstract Glutathione transferases (GSTs) catalyze the conjugation of glutathione (GSH) to endogenous and xenobiotic electrophilic compounds and have been involved in the development of resistance toward cancer chemotherapeutic drugs and in the etiology, pathology and progression of several other diseases. In the present work, the human isoenzyme GSTA1-1 (hGSTA1-1) was used to assemble a microplate-based platform for high-throughput screening of inhibitors from plant extracts. The enzyme was immobilized using sol-gel chemistry and deposited as a layer at the bottom surface of 96-well format ELISA microplate. The se...
Source: Current Pharmaceutical Biotechnology - October 28, 2018 Category: Biotechnology Authors: Chronopoulou EG, Labrou NE Tags: Curr Pharm Biotechnol Source Type: research

Extracellular Production of a Potent and Chemical Resistant Nattokinase in Immobilized Escherichia coli Using Response Surface Methodology.
Abstract Nattokinase is a potent fibrinolytic protease, besides various industrial applications used as a drug for treatment or a supplement for preventing thrombosis. Generally, most of native extracellular proteins such as nattokinase from B.subtilis are lysed by some secretory proteases. Therefore, one way for solving this problem, is to apply other hosts for nattokinase production. There are different factors for producing secretory form of nattokinase from B.subtilis, such as suitable host, optimized media for the maximum activity and easy purification which are studied in this investigation. E.coli BL21 (DE3...
Source: Current Pharmaceutical Biotechnology - October 22, 2018 Category: Biotechnology Authors: Mohammadi F, Nezafat N, Berenjian A, Negahdaripour M, Zamani M, Ghoshoon MB, Morowvat MH, Hemmati S, Ghasemi Y Tags: Curr Pharm Biotechnol Source Type: research

Comparative Molecular Characterization to Reveal Surface Behaviour of Non-proteolytic Bromelain Mutants.
Abstract Rheumatoid Arthritis (RA) is a chronic autoimmune disease that results in the systemic inflammation principally affecting the capsule covering the articulating ends of the synovial joints. Pharmacological treatment involving analgesics and anti-inflammatory drugs including steroids suppress the symptoms and have no effect on disease progression. However, disease modifying anti rheumatic drugs (DMARD) used for the treatment were still analysed for their long term effects. Bromelain has been widely used as phytotherapeutic drug owing to its anti-inflammatory, analgesic, anti-tumor and fibrinolytic propertie...
Source: Current Pharmaceutical Biotechnology - October 17, 2018 Category: Biotechnology Authors: Rajan GS, Sharma A, Kumari P, Biswas S Tags: Curr Pharm Biotechnol Source Type: research

Enzymatic hydrolysis of hyaluronic layer before drug dosing.
Abstract Polymeric drug delivery system, allowing the controlled long-term drug release, is a good alternative to the standard way (oral or intravenous) of pharmaceuticals' administration. The most widespread drug release strategy is based on the drug molecules diffusion: first inside the carrier, then through the external layers of carrier and then, outside the carrier, to the cells surrounding. The proposed type of the local therapy is a particularly proper approach in treatment of cancer diseases, at which the drugs are highly toxic. Unfortunately, antitumor drugs diffusion is quite often limited by hyaluronic ...
Source: Current Pharmaceutical Biotechnology - October 17, 2018 Category: Biotechnology Authors: Trusek-Holownia A, Niesobska A, Semba D Tags: Curr Pharm Biotechnol Source Type: research

Leukocyte-Independent Effects of CC-Chemokines on Vascular Remodeling.
Abstract Vascular remodeling is an alteration in the structure of vessels in response to injury or hemodynamic changes. Disturbance of the structural and functional integrity of the endothelial cell layer can be observed in vascular remodeling associated with inflammation. Chemokines have been implicated in a wide range of diseases with prominent inflammatory components, and also in vascular remodeling. Among them, CC-chemokines are of great interest. They act through conventional CC-chemokine receptors (CCRs), widely expressed by leucocytes and especially monocytes, which are attracted to sites of chronic inflamm...
Source: Current Pharmaceutical Biotechnology - October 17, 2018 Category: Biotechnology Authors: Paccosi S, Parenti A Tags: Curr Pharm Biotechnol Source Type: research

Exosome-like nanovesicles isolated from Citrus limon L. exert antioxidative effect.
CONCLUSION: We demonstrated that incubation with EXO-CL exerts antioxidant activity in human cells. This is most likely due to the direct delivery and uptake of micronutrients by human cells that are well preserved inside the nanovesicle membrane, including the unstable vitamin C. Based on our results, we speculate that fruit-derived nanovesicles have the potential to mediate interspecies influence after food intake. PMID: 30332948 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 17, 2018 Category: Biotechnology Authors: Baldini N, Torreggiani E, Roncuzzi L, Perut F, Zini N, Avnet S Tags: Curr Pharm Biotechnol Source Type: research

Circulating endothelial progenitor cells biology and regenerative medicine in pulmonary vascular diseases.
Abstract Pulmonary vascular disorders (PVDs) include primary or secondary diseases who influence the right heart function. Several researches showed that stem and progenitor cells may represent a novel approach in treating pulmonary hypertension. In particular, circulating endothelial progenitor cells (EPCs) are mobilized either from the bone marrow and/or arteries to replace dysfunctional endothelial cells and restore blood perfusion to ischemic tissues. They may deliver paracrine signals to stimulate local angiogenesis or may be physically incorporated within neovessels. Understanding the molecular mechanisms ut...
Source: Current Pharmaceutical Biotechnology - October 17, 2018 Category: Biotechnology Authors: Guerra G, Perrotta F, Testa G Tags: Curr Pharm Biotechnol Source Type: research

Gastroduodenal Disorders in Patients with CVID Undergoing Immuno-globulin Therapy.
CONCLUSION: In our cohort of CVID patients, gastro-duodenal histopathological findings, including ma-lignancies, are frequent and can affect long-term prognosis. A rigorous endoscopic follow-up is needed in CVID patients irrespective of the gastrointestinal symptoms. PMID: 30336770 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 10, 2018 Category: Biotechnology Authors: Varricchi G, Pecoraro A, Crescenzi L, Marone G, Travaglino A, D'Armiento FP, Genovese A, Spadaro G Tags: Curr Pharm Biotechnol Source Type: research

Probiotic supplementation and topical application in the treatment of pediatric atopic dermatitis.
Abstract Atopic dermatitis is a chronic inflammatory skin disease that commonly presents in early childhood. To date, the pharmacological treatment of atopic dermatitis is far from ideal, poses several limitations and constantly requires novel approaches. The theory that appropriate colonization of gut bacteria during infancy, influences the development of the immune system has initiated numerous clinical trials that evaluated the effectiveness of probiotic supplementation for the prevention and treatment of atopic eczema in children. In addition, topical administration of probiotics has demonstrated the ability t...
Source: Current Pharmaceutical Biotechnology - October 8, 2018 Category: Biotechnology Authors: Marcinkowska M, Zagorska A, Fajkis N, Kołaczkowski M, Pasko P Tags: Curr Pharm Biotechnol Source Type: research

Identification and characterization of genes in curcuminoid pathway of Curcuma zedoaria Roscoe.
CONCLUSION: The sequence information of DCS and CURS1-3 genes in C. zedoaria will be very valuable for subsequent study on the effects of elicitors on the transcription of genes involving in curcuminoid biosynthesis pathway. PMID: 30295188 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - October 8, 2018 Category: Biotechnology Authors: Lan TTP, Huy ND, Luong NN, Nghi NV, Tan TH, Quan LV, Loc NH Tags: Curr Pharm Biotechnol Source Type: research

Circulating endothelial progenitor cells biology and regenerative medicine in pulmonary vascular diseases.
Abstract Pulmonary vascular disorders (PVDs) include primary or secondary diseases who ultimately influence the right heart function. Several researches showed that stem and progenitor cells may represent a novel approach in treating pulmonary hypertension. In particular, circulating endothelial progenitor cells (EPCs) are mobilized either from the bone marrow and/or arteries to replace dysfunctional endothelial cells and restore blood perfusion to ischemic tissues. They may deliver paracrine signals to stimulate local angiogenesis or may be physically incorporated within neovessels. Understanding the molecular me...
Source: Current Pharmaceutical Biotechnology - September 27, 2018 Category: Biotechnology Authors: Guerra G, Perrotta F, Testa G Tags: Curr Pharm Biotechnol Source Type: research

Dietary Patterns: A new therapeutic approach for depression?
CONCLUSION: More large scale randomized clinical trials need to be conducted, in order to confirm the association between high quality dietary patterns and lower risk of depression and depressive symptoms. PMID: 30255747 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - September 25, 2018 Category: Biotechnology Authors: Jesus M, Silva T, Cagigal C, Martins V, Silva C Tags: Curr Pharm Biotechnol Source Type: research

Does diet affect the symptoms of ADHD?
CONCLUSION: There is no clear evidence that supports dietary interventions for the treatment of ADHD. The effects of unhealthy diet patterns in ADHD individuals are not yet fully understood and, like the general population, children with ADHD may benefit from a healthy lifestyle. PMID: 30255748 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - September 25, 2018 Category: Biotechnology Authors: Cagigal C, Silva T, Jesus M, Silva C Tags: Curr Pharm Biotechnol Source Type: research

Food components with potential to be used in the therapeutic approach of mental diseases.
CONCLUSION: More research is needed to prove the therapeutic potential of the food components based on scientific evidence, also on intervention studies to demonstrate the improvement of neuronal and cognitive impairments. PMID: 30255749 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - September 25, 2018 Category: Biotechnology Authors: Frutos MJ, Valero-Cases E, Rincon-Frutos L Tags: Curr Pharm Biotechnol Source Type: research

Food with influence in the sexual and reproductive health.
CONCLUSION: The compiled results indicate that despite the multifactorial etiology of sexual/reproductive dysfunction, nutritional factors may affect the sexual and reproductive health in both men and women. However, it is necessary further studies to clarify this association, and simultaneously improve the approach and treatment of patients with sexual and/or reproductive problems. PMID: 30255750 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - September 25, 2018 Category: Biotechnology Authors: Silva T, Jesus M, Cagigal C, Silva C Tags: Curr Pharm Biotechnol Source Type: research

Preeclampsia - Current Management and Future Approach.
Abstract Preeclampsia is one of the most serious pregnancy - specific medical conditions of increasing incidence. It remains a major cause of maternal and fetal morbidity and mortality. Although maternal mortality is especially high in developing countries, preeclampsia and its complications are one of the top four causes of maternal deaths even in developed societies. Unfortunately, yet the only effective and definitive treatment of preeclampsia is delivery of the baby and placenta, and its time depends on the disease severity and gestational age. In this review we report principles of management of preeclampsia ...
Source: Current Pharmaceutical Biotechnology - September 25, 2018 Category: Biotechnology Authors: Dymara-Konopka W, Laskowska M, Oleszczuk J Tags: Curr Pharm Biotechnol Source Type: research

The role of Agonistic Autoantibodies to the Angiotensin II Type 1 Receptor (AT1-AA) in Pathophysiology of Preeclampsia.
Abstract Preeclampsia is the leading cause of death and morbidity world-wide for the mother and fetus during pregnancy. Preeclampsia does not only effect the mother and the baby during pregnancy, but can also have long-term effects, such as the increase risk of hypertension and cardiovascular disease, on the offspring and the postpartum mother later in life. The exact cause of preeclampsia is unknown, but women with preeclampsia have elevated concentrations of agonistic autoantibodies against the angiotensin II type 1 receptor (AT1-AA). These AT1-AA's through multiple studies have shown to play a significant role ...
Source: Current Pharmaceutical Biotechnology - September 25, 2018 Category: Biotechnology Authors: Campbell N, LaMarca B, Cunningham MW Tags: Curr Pharm Biotechnol Source Type: research

Angiogenic imbalance as a contributor of preeclampsia.
Abstract Preeclampsia is one of the most serious pregnancy - specific medical conditions affecting 3-6% of all gestations. It remains a leading cause of maternal and fetal morbidity and mortality. The aetiology of preeclampsia is not fully elucidated yet, although a huge progress has been made in its understanding within the last decade. Numerous studies have provided compelling evidence that an excess of some antiangiogenic molecules released by the placenta to maternal circulation, in particular soluble fms-like tyrosine kinase 1 (sFlt-1) and soluble endoglin (sEng) and decreased levels of proangiogenic substanc...
Source: Current Pharmaceutical Biotechnology - September 25, 2018 Category: Biotechnology Authors: Dymara-Konopka W, Laskowska M, Blazewicz A Tags: Curr Pharm Biotechnol Source Type: research

Endless pursuit :evolution of insulin administration.
Endless pursuit:evolution of insulin administration. Curr Pharm Biotechnol. 2018 Sep 18;: Authors: Sun C, Yu J, Lin K, Han R, Hu L Abstract Diabetes mellitus is a chronic metabolic health condition affecting the steady state of blood sugar level. The usual method of administration is subcutaneous injection of insulin. There are several ways to subcutaneously inject insulin, such as syringes, insulin pens, and insulin pumps. However, subcutaneous injections of insulin can lead to discomfort, pain and local infection. This review focuses on traditional methods of insulin administration, non-invasive a...
Source: Current Pharmaceutical Biotechnology - September 18, 2018 Category: Biotechnology Authors: Sun C, Yu J, Lin K, Han R, Hu L Tags: Curr Pharm Biotechnol Source Type: research

Evidence of 68Ga-DOTA-NT-20.3 uptake in pancreatic adenocarcinoma AsPC-1 cell line - in vitro study.
Conclusion New tracer 68Ga-DOTA-NT-20.3 can be a suitable candidate for the clinical use in patients with pancreatic ductal adenocarcinoma. PMID: 30160211 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 29, 2018 Category: Biotechnology Authors: Marenco M, Lodola L, Persico MG, Frangipane V, Facoetti A, Aprile C, Hodolic M Tags: Curr Pharm Biotechnol Source Type: research

Recent progress and future perspectives of antibiofilm drugs immobilized on nanomaterials.
CONCLUSION: This review article evaluates the current impact of antibiofilm drugs including its delivery, efficiency of blocking cell attachment and molecular mechanisms of action that are conjugated or encapsulated with different types of biocompatible nanomaterials. It will lead to a better understanding of the antibiofilm drugs and their role in combating biofilms. It will also open new doors for the application of immobilized antibiofilm drugs. PMID: 30152281 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 27, 2018 Category: Biotechnology Authors: Khan F, Khan MM, Kim YM Tags: Curr Pharm Biotechnol Source Type: research

Determination of menaquinone-7 by a novel, simplified HPLC method.
CONCLUSION: The general usefulness of the described method is demonstrated by the application of this method in the analysis of MK-7 from Bacillus species. Under these conditions, the analysis of MK-7 was achieved in less than 8 minutes with a retention time of 7.19 ± 0.1 minutes. PMID: 30152282 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 27, 2018 Category: Biotechnology Authors: Ranmadugala D, Grainger M, Manley-Harris M, Berenjian A Tags: Curr Pharm Biotechnol Source Type: research

Apoptosis: Mediator molecules, interplay with other cell death processes and therapeutic potentials.
Abstract Apoptosis, a form of programmed cell death, plays a very crucial role in various physiological processes for maintaining the homeostasis. This process is characterized by membrane blebbing, nuclear condensation, DNA fragmentation and cell shrinkage. Any defect in this highly regulated process eventually leads to extended cell survival and could result in neoplastic cell expansion followed by genetic instability. The apoptotic machinery is mainly processed and regulated by various caspases, a family of cysteine proteases. Significant advancement has been made towards understanding the molecular mechanisms ...
Source: Current Pharmaceutical Biotechnology - August 20, 2018 Category: Biotechnology Authors: Barman J, Kumar R, Saha G, Tiwari K, Dubey VK Tags: Curr Pharm Biotechnol Source Type: research

Antioxidant, antimicrobial and the phenolic content of infusion, decoction and methanolic extracts of Thyme and Rosmarinus species.
CONCLUSIONS: This study showed important bioactivities (antioxidant, antimicrobial, and safety potential) of the plant species RO, TA and TC used in traditional medicine. PMID: 30117391 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 17, 2018 Category: Biotechnology Authors: Zairi A, Nouir S, M'hamdi N, Bennani M, Bergaoui I, Mtiraoui A, Chaouachi M, Trabelsi M Tags: Curr Pharm Biotechnol Source Type: research

The impact of Antiepileptic drugs on Vitamins levels in epileptic patients.
CONCLUSION: The most significant and surprising finding of this study revealed that serum vitamin D levels in particular were very low in almost all patients and in some patients vitamin B1 serum levels were also below the reference range. More importantly, it is first time reported here that vitamin C serum levels were also below reference range in the majority of these Chinese epileptic patients. It is recommended that all these vitamins should be regularly monitored in addition to therapeutic drug monitoring of antiepileptic drugs. Additional clinical trials are required for further evaluation. It is also recommended th...
Source: Current Pharmaceutical Biotechnology - August 15, 2018 Category: Biotechnology Authors: Shaikh AS, Guo X, Li Y, Cao L, Liu X, Li P, Zhang R, Guo R Tags: Curr Pharm Biotechnol Source Type: research

Aquacultural, Nutritional and Therapeutic Biology of Delicious Seeds of Euryale ferox Salisb. : A minireview.
CONCLUSION: Active research is required to harness this macro-hydrophyte along with co-culture of compatible fish varieties and other hydrophytes into sustainable livelihood and economic prosperity. PMID: 30091404 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 8, 2018 Category: Biotechnology Authors: Jha V, Shalini R, Kumari A, Jha P, Sah NK Tags: Curr Pharm Biotechnol Source Type: research

Manipulating intracellular Ca2+ signals to stimulate therapeutic angiogenesis in cardiovascular disorders.
Abstract Endothelial progenitor cells (EPCs) are mobilized in peripheral blood to rescue blood perfusion in ischemic tissues. Several approaches were, therefore, designed to inject autologous EPCs and induce therapeutic angiogenesis in patients affected by cardiovascular disorders. Endothelial colony forming cells (ECFCs) represent the only truly endothelial precursor and are regarded as the most suitable substrate for cell based therapy of ischemic diseases. Intracellular Ca2+ signalling drives ECFC proliferation, migration, homing and neovessel formation. Vascular endothelial growth factor (VEGF) triggers repeti...
Source: Current Pharmaceutical Biotechnology - August 8, 2018 Category: Biotechnology Authors: Moccia F, Berra-Romani R, Rosti V Tags: Curr Pharm Biotechnol Source Type: research

Role of NLRP-3 Inflammasome in Hypertension: a Potential Therapeutic Target.
Conclusion The inflammasome represents a leading promoter of hypertensive inflammation opening new perspective in the field of the clinical approach in this pathology. PMID: 30091406 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 8, 2018 Category: Biotechnology Authors: Pasqua T, Pagliaro P, Rocca C, Angelone T, Penna C Tags: Curr Pharm Biotechnol Source Type: research

Validation of calculated globulin (CG) as a screening test for antibody deficiency in an Italian University Hospital.
This study investigated the possibility to use low levels of CG to detect antibody deficiency in an Italian University Hospital. First, we conducted an analysis of anonymized adult samples collected at our biochemistry laboratory with a range of calculated globulin levels from 15 to 22 g/l. A CG cut-off of 19 g/l detected subjects with IgG lower than 600 mg/dl with a sensitivity of 70% and a specificity of 75%. To further verify the clinical usefulness of CG, we retrospectively evaluated the relationship between CG values and serum IgG levels in 38 patients diagnosed with CVID at our Institution. Using a CG cut-off of 19 g...
Source: Current Pharmaceutical Biotechnology - August 8, 2018 Category: Biotechnology Authors: Pecoraro A, Jolles S, Crescenzi L, Varricchi G, Marone G, Savoia M, Genovese A, Spadaro G Tags: Curr Pharm Biotechnol Source Type: research

The role of Transforming growth factor- β1 gene polymorphism and its serum levels in Hashimoto's thyroiditis.
CONCLUSION: We suggest that heterozygous genotype CT is a genetic risk factor for developing more severe HT due to enhanced free T4 serum level at the onset of the disease, before developing the hypothyroid stage. PMID: 30070177 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 2, 2018 Category: Biotechnology Authors: Stanilova SA, Gerenova JB, Miteva LD, Manolova I Tags: Curr Pharm Biotechnol Source Type: research

Small activating RNAs: towards development of new therapeutic agents and clinical treatments.
CONCLUSION: Accordingly, this article aims to provide insights into how RNAa cellular machinery can be manipulated to activate gene expression and for more effective clinical treatments of diverse diseases. PMID: 30070178 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - August 2, 2018 Category: Biotechnology Authors: Li SM, Hu J Tags: Curr Pharm Biotechnol Source Type: research

Clinical management of drug resistant Mycobacterium tuberculosis strains: Pathogen-targeted versus host-directed treatment approaches.
DISCUSSION: This review discusses the main suggestions proposed so far to resolve the clinical challenges associated with drug resistance, in the context of TB. These suggestions include novel drug delivery approaches that could optimize treatment outcome and increase patients' compliance to the treatment. PMID: 30062961 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 31, 2018 Category: Biotechnology Authors: Al-Ghafli H, Al-Hajoj S Tags: Curr Pharm Biotechnol Source Type: research

Functionalization of inorganic nanoparticles to augment antimicrobial efficiency: a critical analysis.
CONCLUSION: In this review we describe functionalized NPs as an alternative for targeting MDR bacteria, their mode of action and future directions that are necessary to move forward with this approach. PMID: 30062962 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 31, 2018 Category: Biotechnology Authors: Khan K, Javed S Tags: Curr Pharm Biotechnol Source Type: research

The release of perillyl alcohol from the different kind of vehicles.
Abstract BACKGROUND: Skin cancers are the most common malignancy of humans, and the number of cases has increased dramatically in the past few decades. Therefore, it is very important to carry out studies concerning new and safer anticancer natural agents (e.g. perillyl alcohol) and modern drug delivery systems, such as nanoformulations, which increase their bioavailability. OBJECTIVES: The aim of this work was to obtain different kinds of topical vehicles formulation and compare their efficiency in the release of perillyl alcohol. The release kinetics was determined by using certain selected mathematical mod...
Source: Current Pharmaceutical Biotechnology - July 30, 2018 Category: Biotechnology Authors: Miastkowska M, Konieczna M, Lason E, Tabaszewska M, Sikora E, Ogonowski J Tags: Curr Pharm Biotechnol Source Type: research

Forage Legumes as Sources of Bioactive Phytoestrogens for Use in Pharmaceutics: A Review.
CONCLUSION: Available evidence suggests that forage legumes represent high promising sources of health promoting phytoestrogens. Due to numerous favorable features they can find commercial application in different industries such as pharmaceutical, nutraceutical, cosmetic, and agriculture. PMID: 30062960 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 30, 2018 Category: Biotechnology Authors: Tucak M, Horvat D, Cupic T, Krizmanic G, Tomas V, Ravlic M, Popovic S Tags: Curr Pharm Biotechnol Source Type: research

Changes In The Expression Profile Of Jak/Stat Signaling Pathway Genes And Mirnas Regulating Their Expression Under The Adalimumab Therapy.
CONCLUSION: It can be observed that miRNAs play extremely important role in the regulation of the expression of genes associated with JAK/STAT signaling pathway. The results of this study show the possibility of using changes in mRNAs and miRNAs profile expression, as complementary molecular markers of adalimumab treatment effectiveness. PMID: 30058482 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 29, 2018 Category: Biotechnology Authors: Grabarek B, Wcislo-Dziadecka D, Gola J, Kruszniewska-Rajs C, Brzezinska-Wcislo L, Zmarzly N, Mazurek U Tags: Curr Pharm Biotechnol Source Type: research

New Sources of Calcium (Chicken Eggshells, Chelates) - Preparation of Raw Material and Tablets.
CONCLUSION: In turn, calcium carbonate added to calcium bisglycinate statistically significantly delayes the release of calcium ions to 4 hours. PMID: 30033866 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 22, 2018 Category: Biotechnology Authors: Siemiradzka W, Dolinska B, Ryszka F Tags: Curr Pharm Biotechnol Source Type: research

New Developments in Pichia pastoris Expression System, Review and Update.
CONCLUSION: New developments occurred in the P. pastoris expression system converted this system to a versatile host for production of complex proteins. These progresses paved the way for several proteins to enter to the clinical trials or industrial processes from this valuable expression host. PMID: 30019641 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 17, 2018 Category: Biotechnology Authors: Baghban R, Farajnia S, Ghasemi Y, Mortazavi M, Zarghami N, Samadi N Tags: Curr Pharm Biotechnol Source Type: research

Low Doses of G-CSF Prevent Cerebral Infarction and 1 Maintain Muscle Strength in an Experimental Model of 2 Global Ischemic Stroke 3.
CONCLUSIONS: The results indicate a neuroprotective effect of G-CSF, even in small doses, in mice subjected to global cerebral ischemia, thereby reducing the neurofunctional impairment caused by stroke, when considering the maintenance of muscle strength in the treated animals. PMID: 30019642 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 17, 2018 Category: Biotechnology Authors: da Ros Peruch B, Monteiro BL, Aires R, Martins E Silva C, Guimaraes MCC, Vasquez EC, Nogueira BV Tags: Curr Pharm Biotechnol Source Type: research

The drug combination of SB202190 and SP600125 significantly inhibit the growth and metastasis of olaparib-resistant ovarian cancer cell.
CONCLUSION: Our data suggested that aberrant over-expression of P38 and JNK is causally linked to olaparib resistance in ovarian cancer. Combination of P38 and JUN inhibitors demonstrated significantly anti-tumor activity both in vitro and in vivo. Our study highlighted the potential therapeutic value of Mitogen-Activated Protein Kinase (MAPK) inhibitors in olaparib-resistant human ovarian cancer. PMID: 30003858 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 12, 2018 Category: Biotechnology Authors: Chen X, Chen Y, Lin X, Su S, Hou X, Zhang Q, Tian Y Tags: Curr Pharm Biotechnol Source Type: research

HELLP Syndrome: Pathophysiology and Current Therapies.
Abstract BACKGROUND: HELLP syndrome is a disorder associated with serious maternal morbidity and mortality. Distinguishing HELLP from other pregnancy related disorders is often challenging and may result in delay of treatment. Differential diagnoses include acute fatty liver of pregnancy, thrombotic thrombocytopenic purpura, antiphospholipid syndrome, and hemolytic uremic syndrome, and are reviewed in this chapter. While there is not any current treatment for HELLP, the mainstay of treatment involves maternal stabilization and timely delivery. Various treatment strategies have been attempted to help decrease the m...
Source: Current Pharmaceutical Biotechnology - July 11, 2018 Category: Biotechnology Authors: Bean C, Harris S, Addison A, Wallace K Tags: Curr Pharm Biotechnol Source Type: research

Analytical Advances in Clinical and Forensic Toxicology. Second Part.
PMID: 29962330 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 4, 2018 Category: Biotechnology Authors: Pichini S, Busardo FP Tags: Curr Pharm Biotechnol Source Type: research

Preface: A New Era of Nanoimmunology.
PMID: 29962331 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - July 4, 2018 Category: Biotechnology Authors: Prosperi D Tags: Curr Pharm Biotechnol Source Type: research

Luteolin Regulates Macrophage Polarization via the PI3K/Akt Pathway to Inhibit the Apoptosis Stimulated by Angiotensin Ⅱ.
CONCLUSIONS: These results indicated that Lut has anti-apoptotic effects on macrophages stimulated with Ang II through macrophage polarization, which might be associated with the PI3K/Akt signaling pathway. PMID: 29956625 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - June 29, 2018 Category: Biotechnology Authors: Jiang Q, Pan D, Yang Y, Hu Y, Fang L, Shang P, Xia Y, Li D Tags: Curr Pharm Biotechnol Source Type: research

Biotechnological Approaches for Production of Pharmaceutically Important Compound: Plumbagin.
CONCLUSION: Plumbagin is an important phytocompound which shows potential towards treatments of various diseases. Demand for the production of plumbagin continuously increasing worldwide due to its pharmacological properties. To fulfil commercial demand of plumbagin alternative technologies need to be investigated. Biotechnological approaches like cell suspension culture, root suspension culture and hairy root culture are alternative techniques for plumbagin production. These techniques provide continuous supply of bioactive compounds. However, research on various aspects of tissue culture production techniques is in prepa...
Source: Current Pharmaceutical Biotechnology - June 29, 2018 Category: Biotechnology Authors: Roy A, Bharadvaja N Tags: Curr Pharm Biotechnol Source Type: research