Sodium Oleate Increases c in Methylophilus quaylei Biofilms on Teflon and Polypropylene.
Conclusion: The study demonstrates that sodium oleate has a promoting activity against planktonic growth of M. quaylei strains and has a slight inhibitory effect on biofilm. Addition of sodium oleate enhances the bactericidal effect of ampicillin against biofilm cells. Combination of ampicillin 0.1 mg/ml (MIC) and sodium oleate 0.03 mg/ml showed a remarkable destruction effect on established biofilms. Overall, results indicated that sodium oleate in combination with ampicillin enhances the inhibition of M. quaylei biofilms and this combination can be utilized for combating bacterial biofilm resistance. PMID: 30806309 ...
Source: Current Pharmaceutical Biotechnology - February 22, 2019 Category: Biotechnology Authors: Abdelzaher Mohamed AMHA, Vladimirovna SN, Grigorievich ZV, Borisovna PA, Ivanovich SV Tags: Curr Pharm Biotechnol Source Type: research

Comparative evaluation of conventional and novel extracts of stem bark of Terminalia arjuna for antihypertensive activity in BSO induced oxidative stress based rat model.
CONCLUSION: The total percentage yield, the yield of the marker compounds arjunolic and arjunic acid, the IC50 values for antioxidant activity as well as the antihypertensive effect was in order: MAE>USAE>SE>RTE that suggested the role of biomarkers arjunolic acid and arjunic acid in reversing the effect of buthionine sulfoxamine. PMID: 30806310 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 22, 2019 Category: Biotechnology Authors: Khatkar S, Nanda A, Ansari SH Tags: Curr Pharm Biotechnol Source Type: research

Gender Difference Response of Male and Female Immunodeficiency Rats Treated with Tissue-specific Biomolecules.
Abstract BACKGROUND: The modern immunology is targeted to the detailed study of various immunopathological conditions at the molecular and cellular level, development of new methods for prevention, diagnostics and treatment of contagious and non-contagious diseases of humans and animals. METHODS: In the present work we took the rats with model of cyclophosphamide-induced immunodeficiency and studied the features of the gender impact of the complex extract of immunocompetent organs (thymus, spleen and mesenteric lymph nodes) Sus scrofa and its separate fraction with molecular weight less than 30 kDa administer...
Source: Current Pharmaceutical Biotechnology - February 22, 2019 Category: Biotechnology Authors: Fedulova LV, Basov AA, Vasilevskaya ER, Dzhimak SS Tags: Curr Pharm Biotechnol Source Type: research

Expression of NRP-1 and NRP-2 in Endometrial Cancer.
CONCLUSION: NRP1 and NRP2 are candidates for complementary diagnostic molecular markers and promising new targets for molecular, personalized anticancer therapies. PMID: 30806307 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 19, 2019 Category: Biotechnology Authors: Oplawski M, Dziobek K, Grabarek B, Zmarzly N, Dabrus D, Januszyk P, Brus R, Tomala B, Boron D Tags: Curr Pharm Biotechnol Source Type: research

Phytosterols and Triterpenoids for Prevention and Treatment of Metabolic- related Liver Diseases and Hepatocellular Carcinoma.
Conclusion: Plant and food-derived sterols and triterpenoids (TTP) possess antioxidant, metabolic-regulating, immunomodulatory, and anti-inflammatory activities, as well as they are recognized as anticancer agents, suggesting their application strongly as an alternative therapy in some chronic diseases. Thus, it is interesting to review current reports about them as hepatoprotective agents, but also because they structurally resemble cholesterol, sexual hormones, corticosteroids and bile acids due to the presence of the steroid nucleus, so they all can share pharmacological properties through activating nuclear and membran...
Source: Current Pharmaceutical Biotechnology - February 19, 2019 Category: Biotechnology Authors: Isabel SC, Eduardo FM, Raquel CC, Gabriel BC, Rosa BA Tags: Curr Pharm Biotechnol Source Type: research

A Novel Oral Glutarimide Derivative XC8 Suppresses Sephadex-Induced Lung Inflammation in Rats and Acute Asthma in Guinea Pigs.
CONCLUSION: The anti-asthmatic effect of XC8 is comparable to that of budesonide. Moreover, in contrast to budenoside treatment XC8 is capable of reducing of airway resistance and degranulating mast cell numbers in the airway tissue. PMID: 30767739 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 14, 2019 Category: Biotechnology Authors: Ferko B, Romanova J, Rydlovskaya AV, Kromova TA, Proskurina OV, Amelina AN, Schmutz H, Renner A, Nebolsin VE Tags: Curr Pharm Biotechnol Source Type: research

Separation, Characterization and Discriminant Analysis of Subvisible Particles in Biologics Formulations.
Abstract The presence of subvisible particles (SVPs) in parenteral formulations of biologics is a major challenge in the development of therapeutic protein formulations. Distinction between proteinaceous and non-proteinaceous SVPs is vital in monitoring formulation stability. The current compendial method based on light obscuration (LO) has limitations in the analysis of translucent/low refractive index particles. A number of attempts have been made to develop an unambiguous method to characterize SVPs, albeit with limited success. Herein, we describe a robust method that characterizes and distinguishes both poten...
Source: Current Pharmaceutical Biotechnology - February 13, 2019 Category: Biotechnology Authors: Akhunzada ZS, Hubert M, Sahin E, Pratt J Tags: Curr Pharm Biotechnol Source Type: research

Nutritional culturomics and big data: Macroscopic patterns of change in food, nutrition and diet choices.
Abstract Nutritional culturomics (NCs) is a specific focus area of culturomics epistemology developing digital humanities and computational linguistics approaches to search for macro-patterns of public interest in food, nutrition and diet choice as a major component of cultural evolution. Cultural evolution is considered as driver at the interface of environmental and food science, economy and policy. The paper presents an epistemic programme that builds on the use of big data from web-based services such as Google Trends, Google Adwords or Google Ngram Viewer. A comparison of clearly defined NCs in terms of geogr...
Source: Current Pharmaceutical Biotechnology - February 11, 2019 Category: Biotechnology Authors: Troumbis AY, Hatziantoniou M, Vasios GK Tags: Curr Pharm Biotechnol Source Type: research

Valorization of crude glycerol, residue deriving from biodiesel-production process, with the use of wild-type new isolated Yarrowia lipolytica strains: production of metabolites with pharmaceutical and biotechnological interest.
Abstract Crude glycerol (Glol), used as substrate for screening eleven natural Yarrowia lipolytica strains in shake-flask experiments. Aim of this study was to assess the ability of the screened strains to produce biomass (dry cell weight; X), lipid (L), citric acid (Cit), mannitol (Man), arabitol (Ara) and erythritol (Ery), compounds presenting pharmaceutical and biotechnological interest, in glycerol-based nitrogen-limited media, in which initial glycerol concentration had been adjusted to 40 g/L. Citric acid may find use in biomedical engineering (i.e. drug delivery, tissue engineering, bioimaging, orthopedics,...
Source: Current Pharmaceutical Biotechnology - February 11, 2019 Category: Biotechnology Authors: Sarris D, Sampani Z, Rapti A, Papanikolaou S Tags: Curr Pharm Biotechnol Source Type: research

Antitumoral and anticholinesterasic activity of the seven species from Rubiaceae.
CONCLUSION: Herein, the anticholinesterasic and antiproliferative activity of extracts produced from species of Psychotria and Palicourea genus with anticholinesterasic and antiproliferative activity is reported. PMID: 30747062 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 11, 2019 Category: Biotechnology Authors: Volobuff CRF, de Oliveira PC, Dos Santos SM, Pereira ZV, Ferreira DC, Cardoso CAL, Ruiz ALTG, Foglio MA, de Carvalho JE, Formagio ASN Tags: Curr Pharm Biotechnol Source Type: research

Improve Healthcare quality through Mortality Committee: Retrospective analysis of Bambino Ges ù Children Hospital's ten years' experience 2008-2017.
CONCLUSION: Introduction of the mortality review committee has proved to be a valid instrument to improve the quality of the care provided in a hospital, allowing early identification of care gaps that could lead to an increase in mortality rates. PMID: 30747063 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 11, 2019 Category: Biotechnology Authors: Offidani C, Lodise M, Gatto V, Frati P, D'Errico S, Degli Atti MLC, Raponi M Tags: Curr Pharm Biotechnol Source Type: research

Evaluation of Activity Kinetic Parameters of SK319cys, As a New Cysteine Variant of Streptokinase: A Comparative Study.
CONCLUSION: SK319cys, as the new cysteine variant of streptokinase, together with SK263cys and SK45cys can be considered as appropriate molecules for specific PEGylation. PMID: 30734674 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 8, 2019 Category: Biotechnology Authors: Alinodehi NN, Sadeh S, Nezamiha FK, Keramati M, Hasanzadeh M, Mianroodi RA Tags: Curr Pharm Biotechnol Source Type: research

Chemical composition, antioxidant and anti-inflammatory activity evaluation of the Lebanese propolis extract.
CONCLUSION: Lebanese propolis is rich in various amount of flavonoids which showed promising anti-inflammatory and antioxidant properties. Additionally, chemical analysis showed unique chemical compositions with the potential of identifying ingredients with interesting anti-inflammatory activities. PMID: 30727882 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 6, 2019 Category: Biotechnology Authors: Zeitoun R, Najjar F, Wehbi B, Khalil A, Fayyad-Kazan M, Dagher-Hamalian C, Faour WH, El-Makhour Y Tags: Curr Pharm Biotechnol Source Type: research

Downregulation of TdT Expression through Splicing Modulation by Antisense Peptide Nucleic Acid (PNA).
CONCLUSION: Overall, modulation of splicing events with antisense PNAs targeting the TdT pre-mRNA may be a promising route for the discovery of novel anticancer agents. PMID: 30727883 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - February 6, 2019 Category: Biotechnology Authors: Montazersaheb S, Kazemi M, Nabat E, Nielsen PE, Hejazi MS Tags: Curr Pharm Biotechnol Source Type: research

Effect of powdered activated carbon as advanced step in wastewater treatments on antibiotic resistant microorganisms.
a A Abstract Conventional wastewater treatment plants discharge important amounts of antibiotic resistant bacteria and antibiotic resistance genes into natural water bodies contributing to the spread of antibiotic resistance. Some advanced wastewater treatment technologies have been shown to effectively decrease the number of bacteria. Nevertheless, there is still a lack of knowledge about the effectiveness of these treatments on antibiotic resistant bacteria and antibiotic resistant genes. To our knowledge, no specific studies have considered how powdered activated carbon (PAC) treatments can act on antibiotic re...
Source: Current Pharmaceutical Biotechnology - February 6, 2019 Category: Biotechnology Authors: Ravasi D, König R, Principi P, Perale G, Demarta A Tags: Curr Pharm Biotechnol Source Type: research

Establishment of novel cells stably secreting various human IL-18 recombinant proteins.
Abstract The immunotherapies against cancer, autoinmmune diseases or infection are remarkable development. These days programmed cell death (PD)-1 antibody-induced immune checkpoint blockade or chimeric antigen recpetor-T cells (CAR-T) have been shown to have eminent therapeutic effects on tumor development. We have focused on adoptive transfer with human gamma delta T cells for novel immunotherapies. Additionally, IL-18 is one of the cytokines that enhances cytokine secretion and cytotoxicity of human gamma delta T cells. Thus, we established novel cell lines stably expressing and secreting various types of human...
Source: Current Pharmaceutical Biotechnology - February 6, 2019 Category: Biotechnology Authors: Kumagai A, Shimizu K, Kurata R, Cui X, Isagawa T, Harada M, Nagai J, Yoshida Y, Ozaki KI, Takeda N, Yonezawa T Tags: Curr Pharm Biotechnol Source Type: research

Functional Biomolecule Delivery Systems and Bioengineering in Cartilage Regeneration.
Abstract Osteoarthritis (OA) is a common degenerative disease which involves articular cartilage, and leads to total joint disability in the advanced stages. Due to its avascular and aneural nature, damaged cartilage cannot regenerate itself. Stem cell therapy and tissue engineering represent a promising route in OA therapy, in which cooperation of mesenchymal stem cells (MSCs) and three-dimensional (3D) scaffolds contribute to cartilage regeneration. However, this approach still presents some limits such as poor mechanical properties of the engineered cartilage. The natural dynamic environment of the tissue repai...
Source: Current Pharmaceutical Biotechnology - February 6, 2019 Category: Biotechnology Authors: Szychlinska MA, D'Amora U, Ravalli S, Ambrosio L, Rosa MD, Musumeci G Tags: Curr Pharm Biotechnol Source Type: research

Regulation of Vaginal Microbiome by Nitric Oxide.
Abstract In this review, the composition and regulation of vaginal microbiome that displays an apparent microbial diversity and interacts with other microbiota in the body were presented. The role of nitric oxide (NO) in the regulation of vaginal microflora in which lactobacillus species typically dominate has been delineated from the perspective of maintaining gynecologic ecosystem and prevention of onset of bacteriostatic vaginosis (BV) and/or sexually transmitted diseases (STD) including HIV-1 transmission. The interactions between NO and vaginal microbiome and its influence on the levels of Lactobacillus, horm...
Source: Current Pharmaceutical Biotechnology - February 6, 2019 Category: Biotechnology Authors: Yeruva T, Lee CH Tags: Curr Pharm Biotechnol Source Type: research

Theoretical investigation of design space for multi layer drug eluting bioresorbable suture threads.
Abstract The here presented work is focused on the development of a comprehensive model for the description of drug release from a double - layer bioresorbable suture thread and the therapeutic efficacy of the active compounds delivered in the surrounding tissue. In particular, the system under investigation is composed by a core of slow-degrading poly-lactic-acid-co-ε-caprolactone (PLCL), where an antibiotic compound (Vancomycin) is loaded, surrounded by a shell of a fast-degrading polylactic-co-glycolic acid (PLGA) which contains an anesthetic drug (Lidocaine hydrochloride) for the post-surgical pain rel...
Source: Current Pharmaceutical Biotechnology - February 6, 2019 Category: Biotechnology Authors: Casalini T, Rossi F, Brizielli L, Perale G Tags: Curr Pharm Biotechnol Source Type: research

In vivo evaluation and Alzheimer's disease treatment outcome of siRNA loaded dual targeting drug delivery system.
Abstract To deliver drugs to treat Alzheimer's disease (AD), nanoparticles should firstly penetrate through blood brain barrier, and then target neurons. Recently, we developed an Apo A-I and NL4 dual modified nanoparticle (ANNP) to deliver beta-amyloid converting enzyme 1 (BACE1) siRNA. Although promising in vitro results were obtained, the in vivo performance was not clear. Therefore, in this study, we further evaluated the in vivo neuroprotective effect and toxicity of the ANNP/siRNA. The ANNP/siRNA was 80.6 nm with good stability when incubated with serum. The ANNP/siRNA could target both bEnd.3 and PC12 cells...
Source: Current Pharmaceutical Biotechnology - February 4, 2019 Category: Biotechnology Authors: Zhang C, Shen L, Gu Z, Liu X, Lin H Tags: Curr Pharm Biotechnol Source Type: research

Ginger: A novel strategy to battle cancer through modulating cell signalling pathways.
Abstract Numerous studies have been performed in understanding the development of cancer. Though, the mechanism of action of genes in the development of cancer remains to be explained. The current mode of treatment of cancer shows adverse effect on normal cells and also alter the cell signalling pathways. However, ginger and their active compound has fascinated research based on animal model and laboratories during past decade due to its potentiality in killing of cancer cells. Ginger is a mixture of various compound including gingerol, paradol, zingiberene and shogaol and such compound are main player in the dise...
Source: Current Pharmaceutical Biotechnology - January 19, 2019 Category: Biotechnology Authors: Almatroudi A, Alsahli MA, Alrumaihi F, Allemailem KS, Rahmani AH Tags: Curr Pharm Biotechnol Source Type: research

Neutrophil Extracellular Traps as a Drug Target to Counteract Chronic and Acute Inflammation.
Abstract Neutrophil extracellular traps (NET), extruded decondensated chromatin entangled with neutrophil proteases, have been first identified in neutrophils stimulated with bacteria or phorbol myristate acetate (PMA) via activation of NADPH oxidase and the generation of reactive oxygen species. Although the first findings demonstrated the beneficial role of NET formation by trapping the bacteria and limiting their dissemination, numerous studies in the recent decade revealed the multifunctional aspects of NET formation which manifests itself not only in the context of anti-microbial effect but also as a patholog...
Source: Current Pharmaceutical Biotechnology - January 11, 2019 Category: Biotechnology Authors: Saffarzadeh M Tags: Curr Pharm Biotechnol Source Type: research

The Capability to Forecast Response to Therapy with Regard to The Time and Intensity of The Inflammatory Process in vitro in Dermal Fibroblasts Induced by IL-12.
CONCLUSION: The conducted studies indicate that conclusions about the intensity of the inflammatory process, thus the efficacy of the anti-cytokine therapeutic strategy, may be made on the basis of the TNF-α, IFN-γ expression. PMID: 30636601 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - January 11, 2019 Category: Biotechnology Authors: Grabarek B, Wcisło-Dziadecka D, Strzałka-Mrozik B, Adamska J, Mazurek U, Brzezińska-Wcisło L Tags: Curr Pharm Biotechnol Source Type: research

Molecular Identification and Antimicrobial Activity of Vaginal Lactobacillus sp.
CONCLUSION: It was found that the secondary metabolites of some isolates contained both acetic and lactic acid, while some of them contained either acetic or lactic acid. PMID: 30636598 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - January 10, 2019 Category: Biotechnology Authors: Eryilmaz M, Gurpinar SS, Palabiyik IM, Guriz H, Gerceker D Tags: Curr Pharm Biotechnol Source Type: research

Pattern Recognition Receptors and Control of Innate Immunity: Role of Nucleic Acids.
Abstract The innate immune system protects against infectious microbes by the recognition of pathogen- associated molecular patterns, which serve to detect pathogens on the host cell surface or in endosomes by pattern recognition receptors such as Toll-like receptors, nucleotide-binding oligomerization domain-containing protein-1-like receptors, mannose-receptor, or retinoic acid-inducible gene-1- like receptors that initiate proper host defense mechanisms. In addition to pathogen-associated molecular patterns, a series of endogenous danger-associated molecular patterns, such as nucleic acids, are recognized by pa...
Source: Current Pharmaceutical Biotechnology - January 10, 2019 Category: Biotechnology Authors: Fischer S Tags: Curr Pharm Biotechnol Source Type: research

Comparison of Anti-HCV Activity of Multiple Punica granatum Extracts and Fractions in Virus-infected Human Hepatocytes.
CONCLUSION: Our findings suggest that PK AV 003 fruit extract might be useful as an add-on therapeutic candidate for treating HCV infection. PMID: 30608038 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - January 4, 2019 Category: Biotechnology Authors: Rehman S, Ashfaq UA, Ijaz B, Jabeen Z, Riazuddin S Tags: Curr Pharm Biotechnol Source Type: research

Extracellular RNA, a Potential Drug Target for Alleviating Atherosclerosis, Ischemia/Reperfusion Injury and Organ Transplantation.
Abstract Extracellular RNA (eRNA), composed of mainly rRNA e.g. released upon cell injury, has previously been shown to have three main detrimental functions in the context of cardiovascular disease: (1) to promote tissue edema by activating the VEGF signal transduction cascade, disrupting endothelial tight junctions and increasing intercellular permeability; (2) to induce thrombus formation by activating the contact phase system of intrinsic blood coagulation; and (3) to increase inflammation by stimulating leukocyte adhesion and transmigration and the mobilization of pro-inflammatory cytokines. This review propo...
Source: Current Pharmaceutical Biotechnology - January 2, 2019 Category: Biotechnology Authors: Kluever AK, Deindl E Tags: Curr Pharm Biotechnol Source Type: research

Hyperglycemia and diabetes induced by glucocorticoids in nondiabetic and diabetic patients: revision of literature and personal considerations.
CONCLUSIONS: Glucocorticoids-induced diabetes is a harmful and underestimated problem when the therapeutic program is not correct and well planned. Screening or more stringent monitoring must always be suggested before starting with corticosteroids; moreover, the hypoglycemic therapy should be personalized, based on the severity of hyperglycemia, type of steroid used and patients' comorbidities. All the oral glucose-lowering drugs can be used, but insulin is the most studied drug and the medication recommended for severe hyperglycemia, for patients with pre-existing diabetes or for those patients who have to undergo prolon...
Source: Current Pharmaceutical Biotechnology - January 2, 2019 Category: Biotechnology Authors: Elena C, Chiara M, Angelica B, Chiara MA, Laura N, Chiara C, Claudio C, Antonella F, Nicola G Tags: Curr Pharm Biotechnol Source Type: research

The Role of Extracellular Nucleic Acids in Rheumatoid Arthritis.
r U Abstract Chronic inflammation, synovial hyperplasia, and local hypoxia lead to synovial cell activation causing severe joint damage in chronic-inflammatory rheumatoid arthritis (RA). The pro-inflammatory and joint-destructive property of the increased release of extracellular nucleic acids has been demonstrated for extracellular mitochondrial DNA and oxidized DNA using an arthritis model. Microparticles derived from different cells are able to transport nucleic acids to distant cells and promote cellular activation in RA. In addition, extracellular RNA (eRNA) is present in the RA synovial lining layer, whereas...
Source: Current Pharmaceutical Biotechnology - January 2, 2019 Category: Biotechnology Authors: Neumann E, Hasseli R, Lange U, Müller-Ladner U Tags: Curr Pharm Biotechnol Source Type: research

Clinical Trials of Novel Targeted Therapies in Ovarian Cancer: Moving Beyond Poly ADP Ribose Polymerase (PARP) Inhibitors.
DISCUSSION: Implications of on-going phase III trials are: 1) combination therapy of bevacizumab with atezolizumab may be the most anticipated studies for approvals ; 2) mirvetuximab soravtansine plus chemotherapy may generate positive results to justify an approval; and 3) Immune therapy for EOC may bring new treatments for the patients. PMID: 30585545 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - December 26, 2018 Category: Biotechnology Authors: Guo Q, Yang Q, Li J, Liu G, Nikoulin I, Jia S Tags: Curr Pharm Biotechnol Source Type: research

Colorectal Cancer: Causes and Evidence of Chemopreventive Treatments.
Conclusions: The main aim of this review is to provide data regarding the pathophysiological characteristics, molecular mechanisms as well as carcinogenic and chemopreventive agents for CRC, with emphasis on evidence supporting their efficacy. These compounds may modulate multiple signaling pathways involved in cell proliferation and apoptosis in transformed cells, they also enhance the host immune system and favor an effective treatment. Despite promising results from experimental studies, only a limited number of these compounds have been tested in clinical trials. The mechanistic spectrum and specificity of the action o...
Source: Current Pharmaceutical Biotechnology - December 25, 2018 Category: Biotechnology Authors: Emmanuel PE, Raquel CC, Eduardo FM, Mario IO, Manuel MV, Isabel SIBJL, Luis JÁ Tags: Curr Pharm Biotechnol Source Type: research

A recombinant Fab antibody against kwakhurin as a tool for sensitive indirect competitive ELISA.
CONCLUSION: Characterization of anti-Kwa Fab revealed that it possessed high specificity (cross-reactivities with other Kwa-related compounds,
Source: Current Pharmaceutical Biotechnology - December 25, 2018 Category: Biotechnology Authors: Chanpokapaiboon K, Khoonrit P, Yusakul G, Juengwatanatrakul T, Putalun W, Tanaka H, Sakamoto S, Morimoto S Tags: Curr Pharm Biotechnol Source Type: research

Phenolic Acids Derivatives - Biotechnological Methods of Synthesis and Bioactivity.
Abstract Phenolic acids are secondary plant metabolites belonging to polyphenol classes, widely spread throughout the plant kingdom. The name "phenolic acids", in general, describes phenols that possess one carboxylic acid functionality and they could be divided into three major subclasses: hydroxybenzoic, hydroxycinnamic and hydroxyphenylacetic acids. The great interest in phenolic acids is associated with their high potential for food preservation (antioxidant and antimicrobial activity) and, last but not least, high therapeutic potential. The aim of this review is to summarise the current knowledge co...
Source: Current Pharmaceutical Biotechnology - December 17, 2018 Category: Biotechnology Authors: Białecka-Florjańczyk E, Fabiszewska A, Zieniuk B Tags: Curr Pharm Biotechnol Source Type: research

Suppressive Effects of the Standardized Extract of Phyllanthus amarus on Type II collagen-induced Rheumatoid Arthritis in Sprague Dawley Rats.
CONCLUSION: The results suggest that the oral administration of standardized extract of P. amarus was able to suppress the humoral and cellular immune responses to type II collagen, resulting in the reduction of the development of TCIA in the rats. PMID: 30539691 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - December 11, 2018 Category: Biotechnology Authors: Alam J, Jantan I, Kumolosasi E, Nafiah MA, Mesaik MA Tags: Curr Pharm Biotechnol Source Type: research

Optimization of Silver Nanoparticle Synthesis by Banana Peel Extract Using Statistical Experimental Design, and testing of their Antibacterial and Antioxidant Properties.
In this study, silver nanoparticles (AgNPs) were synthesized using Banana Peel Extract (BPE), and characterized using UV- Vis absorbance spectroscopy, X-Ray Powder Diffraction (XRD), Atomic Force Microscopy (AFM), and Fourier Transform Infrared Spectroscopy (FTIR). UV - Vis absorbance spectroscopy showed the characteristic plasmon resonance of AgNPs at 433 nm. Nanoparticle size (between 5 and 9 nm) was measured using AFM, whereas their crystallinity was shown by XRD. FTIR identified the ligands that surround the nanoparticle surface. The synthesis conditions were optimised using Central Composite Design (CCD) under Respons...
Source: Current Pharmaceutical Biotechnology - December 9, 2018 Category: Biotechnology Authors: Rigopoulos N, Thomou E, Kouloumpis Α, Lamprou ER, Petropoulea V, Gournis D, Poulios E, Karantonis HC, Giaouris E Tags: Curr Pharm Biotechnol Source Type: research

Cranberry consumption against urinary tract infections: Clinical state-of-the-art and future perspectives.
CONCLUSIONS: Additional well-designed, double-blind, placebo-controlled clinical trials that use standardized cranberry products for long study periods are strongly recommended in order to determine the efficiency of cranberry on the prevention of UTIs in susceptible populations. At present, cranberry supplementation can safely be suggested as complementary therapy in women with recurrent UTIs. . PMID: 30520372 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - December 5, 2018 Category: Biotechnology Authors: Maria M, Constantinos G Tags: Curr Pharm Biotechnol Source Type: research

Control of aggregation, coaggregation, and liquid droplet of proteins using small additives.
Abstract This review article presents the concepts of aggregates, coaggregates, and liquid droplet of proteins and compares the concentrated states in the presence of small additives to control their formation and dissociation. The aggregates composed of single protein molecules result mainly from hydrophobic interactions between unfolded protein molecules. Thus, the aggregation of protein can be effectively suppressed by small additives that increase the solubility of hydrophobic solutes, typically arginine (Arg) and chaotropes. In contrast, coaggregation that is composed of two or more types of proteins results ...
Source: Current Pharmaceutical Biotechnology - December 3, 2018 Category: Biotechnology Authors: Iwashita K, Mimura M, Shiraki K Tags: Curr Pharm Biotechnol Source Type: research

Improving Bovine Bone Mechanical Characteristics for the Development of Xenohybrid Bone Grafts.
Abstract The further functionalization of natural existing biomaterials is a very efficient method to introduce additional advanced characteristics on a unique structural composition and architecture. As an example, different animal sources, if properly treated, can be used to develop bone xenograft active in hard tissues regeneration. In this sense, it is also important to consider that the selected process has to take into consideration the intrinsic variability of the base material itself and possibly being able to compensate for it. In this work we characterize cancellous bovine bone treated by deposition of p...
Source: Current Pharmaceutical Biotechnology - November 28, 2018 Category: Biotechnology Authors: Cingolani A, Grottoli CF, Esposito R, Villa T, Rossi F, Perale G Tags: Curr Pharm Biotechnol Source Type: research

Expression Profile of Endoglin in Different Grades of Endometrial Cancer.
CONCLUSION: Endoglin can be considered a valuable complementary molecular marker, allowing to visualize the advancement of the cancer process, including endometrial cancer. PMID: 30479213 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 27, 2018 Category: Biotechnology Authors: Opł Awski M, Dziobek K, Adwent I, Dabrus D, Grabarek B, Zmarzł Y N, Plewka A, Boroñ D Tags: Curr Pharm Biotechnol Source Type: research

Enhanced Vitamin K (Menaquinone-7) Production by Bacillus subtilis natto in Biofilm Reactors by Optimization of Glucose-based Medium.
CONCLUSION: These results demonstrate the potential of utilizing biofilm reactors for MK-7 production on an industrial scale. PMID: 30474527 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 26, 2018 Category: Biotechnology Authors: Mahdinia E, Demirci A, Berenjian A Tags: Curr Pharm Biotechnol Source Type: research

Anti-inflammatory, antioxidant and antiproliferative activities from Trichilia silvatica (C.DC).
o ASN Abstract The aim of this research was to investigate the anti-inflammatory, antiproliferative and antioxidant activities of the leaves (MEL-TS) and bark (MEB-TS) from T. silvatica. We evaluated the concentrations of phenolic compounds, condensed tannins, flavonoids, flavonols and HPLC/PDA analysis. MEL-TS and MEB-TS were analysed by LC-PDA, and the results revealed the presence of caffeic acid in both extracts. It showed high total phenolics concentrations (270.8 ± 17.10 mg GAE/ g extract and 278.8 ± 25.13 mg GAE/g extract, respectively), and content of flavonoids in MEL-TS (209.30 ± 2.9...
Source: Current Pharmaceutical Biotechnology - November 23, 2018 Category: Biotechnology Authors: Da Silva JV, Dos Santos RC, Júnior PCO, Pederiva MMC, Kassuya CAL, Cardoso CAL, Pereira ZV, Ruiz ALTG, Foglio MA, De Carvalho JE, Formagio ASN Tags: Curr Pharm Biotechnol Source Type: research

Clinical Efficacy of Combination Intravenous and Intraperitoneal Chemotherapy in the Treatment of Gastric Cancer with Peritoneal Carcinomatosis.
Abstract OBJECTIVE: To identify the clinical and adverse effects of intravenous chemotherapy combined with intraperitoneal chemotherapy in the treatment of gastric cancer with abdominal peritoneal carcinomatosis. METHODS: Retrospective analysis was performed on 49 gastric cancer patients with abdominal peritoneal carcinomatosis. Patients were divided into two groups: patients subjected to intravenous chemotherapy (n = 27) were defined as the Control group and patients subjected to combined intravenous and intraperitoneal chemotherapy (n = 22) were defined as the Combination Therapy group. Then the objective e...
Source: Current Pharmaceutical Biotechnology - November 14, 2018 Category: Biotechnology Authors: Zhang S, Feng R, Pan Z, Lin M, Huang X, Jiang T, Xu Q, Chen Q, Yang S Tags: Curr Pharm Biotechnol Source Type: research

Small Activating RNAs as Promising Agents for Biotechnological Use.
Abstract PMID: 30417767 [PubMed - in process] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 13, 2018 Category: Biotechnology Authors: Vaschetto LM Tags: Curr Pharm Biotechnol Source Type: research

Cinnamate Hybrids: A unique family of compounds with multiple biological activities.
Abstract Cinnamic acid derivatives are widely distributed in nature presenting a wide range of biological activities: antiseptic, stimulant, carminative and insecticide activities. Hydroxy cinnamic acids especially caffeic, ferulic chlorogenic present various biological activities and they are synthesized in nature through the phenyl propanoid pathway. The cinnamic moiety is still present in various drugs such as a) Ozagrel, an imidazole π-substituted cinnamic acid acting as a thromboxane A2 synthase inhibitor, used therapeutically for treating acute ischemic stroke, b) Cinnarizine, Cinanserin, Tranilasta serie...
Source: Current Pharmaceutical Biotechnology - November 11, 2018 Category: Biotechnology Authors: Pontiki E, Peperidou A, Fotopoulos I, Hadjipavlou-Litina D Tags: Curr Pharm Biotechnol Source Type: research

Effect of vinca alkaloids on the expression levels of microRNAs targeting apoptosis-related genes in breast cancer cell lines.
Conclusion - This study suggests the possible involvement of miR-222-3p expression in breast cancer cell apoptosis, triggered by vincristine, vinblastine, and vinorelbine. PMID: 30417784 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 11, 2018 Category: Biotechnology Authors: Mavrogiannis AV, Kokkinopoulou I, Kontos CK, Sideris DC Tags: Curr Pharm Biotechnol Source Type: research

Evidence for L-Dopa decarboxylase activity in cancer cell cytotoxicity induced by Docetaxel and Mitoxantrone.
Conclusion - This study suggests the possible involvement of DDC expression in Docetaxel and Mitoxantrone-induced cytotoxicity and apoptosis. PMID: 30417785 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 11, 2018 Category: Biotechnology Authors: Kalantzis ED, Scorilas A, Vassilacopoulou D Tags: Curr Pharm Biotechnol Source Type: research

In vitro bioaccessibility, bioavailability, and the plasma protein interaction of new oral anticoagulants in presence of macronutrients.
CONCLUSION: Dabigatran, rivaroxaban and apixaban may be administered independently of a standard meal, provided that it does not include a high amount of dietary fibers. PMID: 30417786 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 11, 2018 Category: Biotechnology Authors: Raiola A, Tenore GC, Ritieni A, Santomauro M, Ciampaglia R, Novellino E Tags: Curr Pharm Biotechnol Source Type: research

Molecular effects of treatment of human colorectal cancer cells with natural and classical chemotherapeutic drugs: alterations in the expression of apoptosis-related BCL2 family members, including BCL2L12.
Conclusion - The intrinsic apoptotic pathway is activated during treatment of colorectal cancer cells with common chemotherapeutic drugs. Moreover, BCL2L12 mRNA expression increases progressively during treatment, similarly to the expression of other BCL2 family genes favoring apoptosis and/or particular proapoptotic transcripts, thus suggesting a proapoptotic role for BCL2L12 in chemotherapy-treated colorectal cancer. PMID: 30417787 [PubMed - as supplied by publisher] (Source: Current Pharmaceutical Biotechnology)
Source: Current Pharmaceutical Biotechnology - November 11, 2018 Category: Biotechnology Authors: Kontos CK, Avgeris M, Vassilacopoulou D, Ardavanis A, Scorilas A Tags: Curr Pharm Biotechnol Source Type: research

Scorpion toxins targeting voltage-gated sodium channels associated with pain.
Abstract Pain affects approximately 30% of people and places a large economic and social burden on society. Despite the availability of a range of analgesics, complete alleviation of symptoms are still rarely occurred. Effective and safe drugs for the treatment of pain are still an unmet clinical need. In recent years, the voltage-gated sodium channels (VGSCs) have been recognized as potential targets for analgesic development. VGSCs are major players in generating and propagating action potentials. They represent an appealing target for the development of new and safer drugs in the treatment of pain. The majority...
Source: Current Pharmaceutical Biotechnology - November 5, 2018 Category: Biotechnology Authors: Xu Y, Sun J, Liu H, Sun J, Yu Y, Su Y, Cui Y, Zhao M, Zhang J Tags: Curr Pharm Biotechnol Source Type: research

Screening and Identification of ssDNA aptamers against HN protein for detection of bovine parainfluenza virus type 3 antibodies in serum.
Abstract Bovine parainfluenza virus type 3 (BPIV3) is a major but often overlooked pathogen that causes respiratory disease in cattle, especially during transportation and in feedlot situations. In the present study, ssDNA aptamers with high affinity and specificity against the HN protein of BPIV3 were selected using microplates as the matrix. After eleven rounds selection, thirty-four different DNA sequences were obtained in total, wherein w-32, w-33, and w-34 were repeated seven, eleven, and nine times, and with Kd values of 56.57 ± 2.7 nM, 24.64 ± 2.84 nM, and 31.3 ± 3.32 nM, respectively. ...
Source: Current Pharmaceutical Biotechnology - October 31, 2018 Category: Biotechnology Authors: Cheng J, Wang JW, Liu Y, Wu QM, Wang Z Tags: Curr Pharm Biotechnol Source Type: research