Multilayered regulation of amino acid metabolism in Escherichia coli
Curr Opin Microbiol. 2023 Dec 6;77:102406. doi: 10.1016/j.mib.2023.102406. Online ahead of print.ABSTRACTAmino acid metabolism in Escherichia coli has long been studied and has established the basis for regulatory mechanisms at the transcriptional, posttranscriptional, and posttranslational levels. In addition to the classical signal transduction cascade involving posttranslational modifications (PTMs), novel PTMs in the two primary nitrogen assimilation pathways have recently been uncovered. The regulon of the master transcriptional regulator NtrC is further expanded by a small RNA derived from the 3´UTR of glutamine syn...
Source: Current Opinion in Microbiology - December 7, 2023 Category: Microbiology Authors: Masatoshi Miyakoshi Source Type: research
Unlocking the potential of optogenetics in microbial applications
Curr Opin Microbiol. 2023 Nov 30;77:102404. doi: 10.1016/j.mib.2023.102404. Online ahead of print.ABSTRACTOptogenetics is a powerful approach that enables researchers to use light to dynamically manipulate cellular behavior. Since the first published use of optogenetics in synthetic biology, the field has expanded rapidly, yielding a vast array of tools and applications. Despite its immense potential for achieving high spatiotemporal precision, optogenetics has predominantly been employed as a substitute for conventional chemical inducers. In this short review, we discuss key features of microbial optogenetics and highligh...
Source: Current Opinion in Microbiology - December 1, 2023 Category: Microbiology Authors: Moritz Benisch Stephanie K Aoki Mustafa Khammash Source Type: research
Unlocking the potential of optogenetics in microbial applications
Curr Opin Microbiol. 2023 Nov 30;77:102404. doi: 10.1016/j.mib.2023.102404. Online ahead of print.ABSTRACTOptogenetics is a powerful approach that enables researchers to use light to dynamically manipulate cellular behavior. Since the first published use of optogenetics in synthetic biology, the field has expanded rapidly, yielding a vast array of tools and applications. Despite its immense potential for achieving high spatiotemporal precision, optogenetics has predominantly been employed as a substitute for conventional chemical inducers. In this short review, we discuss key features of microbial optogenetics and highligh...
Source: Current Opinion in Microbiology - December 1, 2023 Category: Microbiology Authors: Moritz Benisch Stephanie K Aoki Mustafa Khammash Source Type: research
Unlocking the potential of optogenetics in microbial applications
Curr Opin Microbiol. 2023 Nov 30;77:102404. doi: 10.1016/j.mib.2023.102404. Online ahead of print.ABSTRACTOptogenetics is a powerful approach that enables researchers to use light to dynamically manipulate cellular behavior. Since the first published use of optogenetics in synthetic biology, the field has expanded rapidly, yielding a vast array of tools and applications. Despite its immense potential for achieving high spatiotemporal precision, optogenetics has predominantly been employed as a substitute for conventional chemical inducers. In this short review, we discuss key features of microbial optogenetics and highligh...
Source: Current Opinion in Microbiology - December 1, 2023 Category: Microbiology Authors: Moritz Benisch Stephanie K Aoki Mustafa Khammash Source Type: research
Unlocking the potential of optogenetics in microbial applications
Curr Opin Microbiol. 2023 Nov 30;77:102404. doi: 10.1016/j.mib.2023.102404. Online ahead of print.ABSTRACTOptogenetics is a powerful approach that enables researchers to use light to dynamically manipulate cellular behavior. Since the first published use of optogenetics in synthetic biology, the field has expanded rapidly, yielding a vast array of tools and applications. Despite its immense potential for achieving high spatiotemporal precision, optogenetics has predominantly been employed as a substitute for conventional chemical inducers. In this short review, we discuss key features of microbial optogenetics and highligh...
Source: Current Opinion in Microbiology - December 1, 2023 Category: Microbiology Authors: Moritz Benisch Stephanie K Aoki Mustafa Khammash Source Type: research
Unlocking the potential of optogenetics in microbial applications
Curr Opin Microbiol. 2023 Nov 30;77:102404. doi: 10.1016/j.mib.2023.102404. Online ahead of print.ABSTRACTOptogenetics is a powerful approach that enables researchers to use light to dynamically manipulate cellular behavior. Since the first published use of optogenetics in synthetic biology, the field has expanded rapidly, yielding a vast array of tools and applications. Despite its immense potential for achieving high spatiotemporal precision, optogenetics has predominantly been employed as a substitute for conventional chemical inducers. In this short review, we discuss key features of microbial optogenetics and highligh...
Source: Current Opinion in Microbiology - December 1, 2023 Category: Microbiology Authors: Moritz Benisch Stephanie K Aoki Mustafa Khammash Source Type: research
Nutritional control of bacterial DNA replication
Curr Opin Microbiol. 2023 Nov 29;77:102403. doi: 10.1016/j.mib.2023.102403. Online ahead of print.ABSTRACTAll cells must ensure precise regulation of DNA replication initiation in coordination with growth rate and in response to nutrient availability. According to a long-standing model, DNA replication initiation is tightly coupled to cell mass increase in bacteria. Despite controversies regarding this model, recent studies have provided additional support of this idea. The exact molecular mechanisms linking cell growth with DNA replication under different nutrient conditions remain elusive. However, recent studies in Caul...
Source: Current Opinion in Microbiology - November 30, 2023 Category: Microbiology Authors: Joel Hallgren Kristina Jonas Source Type: research
Nutritional control of bacterial DNA replication
Curr Opin Microbiol. 2023 Nov 29;77:102403. doi: 10.1016/j.mib.2023.102403. Online ahead of print.ABSTRACTAll cells must ensure precise regulation of DNA replication initiation in coordination with growth rate and in response to nutrient availability. According to a long-standing model, DNA replication initiation is tightly coupled to cell mass increase in bacteria. Despite controversies regarding this model, recent studies have provided additional support of this idea. The exact molecular mechanisms linking cell growth with DNA replication under different nutrient conditions remain elusive. However, recent studies in Caul...
Source: Current Opinion in Microbiology - November 30, 2023 Category: Microbiology Authors: Joel Hallgren Kristina Jonas Source Type: research
Matching the β-oxidation gene repertoire with the wide diversity of fatty acids
Curr Opin Microbiol. 2023 Nov 21;77:102402. doi: 10.1016/j.mib.2023.102402. Online ahead of print.ABSTRACTBacteria can use fatty acids (FAs) from their environment as carbon and energy source. This catabolism is performed by the enzymes of the well-known β-oxidation machinery, producing reducing power and releasing acetyl-CoA that can feed the tricarboxylic acid cycle. FAs are extremely diverse: they can be saturated or (poly)unsaturated and are found in different sizes. The need to degrade such a wide variety of compounds may explain why so many seemingly homologous enzymes are found for each step of the β-oxidation cyc...
Source: Current Opinion in Microbiology - November 22, 2023 Category: Microbiology Authors: Veronica Schiaffi Fr édéric Barras Emmanuelle Bouveret Source Type: research
Matching the β-oxidation gene repertoire with the wide diversity of fatty acids
Curr Opin Microbiol. 2023 Nov 21;77:102402. doi: 10.1016/j.mib.2023.102402. Online ahead of print.ABSTRACTBacteria can use fatty acids (FAs) from their environment as carbon and energy source. This catabolism is performed by the enzymes of the well-known β-oxidation machinery, producing reducing power and releasing acetyl-CoA that can feed the tricarboxylic acid cycle. FAs are extremely diverse: they can be saturated or (poly)unsaturated and are found in different sizes. The need to degrade such a wide variety of compounds may explain why so many seemingly homologous enzymes are found for each step of the β-oxidation cyc...
Source: Current Opinion in Microbiology - November 22, 2023 Category: Microbiology Authors: Veronica Schiaffi Fr édéric Barras Emmanuelle Bouveret Source Type: research
Matching the β-oxidation gene repertoire with the wide diversity of fatty acids
Curr Opin Microbiol. 2023 Nov 21;77:102402. doi: 10.1016/j.mib.2023.102402. Online ahead of print.ABSTRACTBacteria can use fatty acids (FAs) from their environment as carbon and energy source. This catabolism is performed by the enzymes of the well-known β-oxidation machinery, producing reducing power and releasing acetyl-CoA that can feed the tricarboxylic acid cycle. FAs are extremely diverse: they can be saturated or (poly)unsaturated and are found in different sizes. The need to degrade such a wide variety of compounds may explain why so many seemingly homologous enzymes are found for each step of the β-oxidation cyc...
Source: Current Opinion in Microbiology - November 22, 2023 Category: Microbiology Authors: Veronica Schiaffi Fr édéric Barras Emmanuelle Bouveret Source Type: research
Matching the β-oxidation gene repertoire with the wide diversity of fatty acids
Curr Opin Microbiol. 2023 Nov 21;77:102402. doi: 10.1016/j.mib.2023.102402. Online ahead of print.ABSTRACTBacteria can use fatty acids (FAs) from their environment as carbon and energy source. This catabolism is performed by the enzymes of the well-known β-oxidation machinery, producing reducing power and releasing acetyl-CoA that can feed the tricarboxylic acid cycle. FAs are extremely diverse: they can be saturated or (poly)unsaturated and are found in different sizes. The need to degrade such a wide variety of compounds may explain why so many seemingly homologous enzymes are found for each step of the β-oxidation cyc...
Source: Current Opinion in Microbiology - November 22, 2023 Category: Microbiology Authors: Veronica Schiaffi Fr édéric Barras Emmanuelle Bouveret Source Type: research
Matching the β-oxidation gene repertoire with the wide diversity of fatty acids
Curr Opin Microbiol. 2023 Nov 21;77:102402. doi: 10.1016/j.mib.2023.102402. Online ahead of print.ABSTRACTBacteria can use fatty acids (FAs) from their environment as carbon and energy source. This catabolism is performed by the enzymes of the well-known β-oxidation machinery, producing reducing power and releasing acetyl-CoA that can feed the tricarboxylic acid cycle. FAs are extremely diverse: they can be saturated or (poly)unsaturated and are found in different sizes. The need to degrade such a wide variety of compounds may explain why so many seemingly homologous enzymes are found for each step of the β-oxidation cyc...
Source: Current Opinion in Microbiology - November 22, 2023 Category: Microbiology Authors: Veronica Schiaffi Fr édéric Barras Emmanuelle Bouveret Source Type: research
Matching the β-oxidation gene repertoire with the wide diversity of fatty acids
Curr Opin Microbiol. 2023 Nov 21;77:102402. doi: 10.1016/j.mib.2023.102402. Online ahead of print.ABSTRACTBacteria can use fatty acids (FAs) from their environment as carbon and energy source. This catabolism is performed by the enzymes of the well-known β-oxidation machinery, producing reducing power and releasing acetyl-CoA that can feed the tricarboxylic acid cycle. FAs are extremely diverse: they can be saturated or (poly)unsaturated and are found in different sizes. The need to degrade such a wide variety of compounds may explain why so many seemingly homologous enzymes are found for each step of the β-oxidation cyc...
Source: Current Opinion in Microbiology - November 22, 2023 Category: Microbiology Authors: Veronica Schiaffi Fr édéric Barras Emmanuelle Bouveret Source Type: research
PhoU: a multifaceted regulator in microbial signaling and homeostasis
Curr Opin Microbiol. 2023 Nov 20;77:102401. doi: 10.1016/j.mib.2023.102401. Online ahead of print.ABSTRACTInorganic phosphate (Pi) is a fundamental molecule crucial for numerous biological processes, such as ATP synthesis and phospholipid formation. To prevent cellular toxicity, Pi transport is often linked to counterion transport within the bacterium. This review discusses the multifaceted functions of the PhoU protein in bacterial regulation, focusing on its role in coordinating Pi transport with counterions, controlling polyphosphate accumulation, and regulating secondary metabolite biosynthesis and DNA repair. We also ...
Source: Current Opinion in Microbiology - November 21, 2023 Category: Microbiology Authors: Seungwoo Baek Eun-Jin Lee Source Type: research
