A Feasibility Study for a Novel trans-infraorbital Canal Approach to the Maxillary Nerve in Pigs ( < em > Sus domesticus < /em > )
This study evaluated the feasibility of a novel maxillary nerve block approach. In part I, cadavers were used to determine anatomic landmarks and assess maxillary nerve dye staining by using 0.03 mL kg-1 of a 1:10 mixture of commercial food dye and 0.5% bupivacaine. In part II, 10 additional pig cadavers underwent bilateral ultrasound-guided maxillary nerve blocks by using trans-infraorbital canal needle placement. The maxillary nerve was harvested and scored based on degree of staining (0 and 1, absent or incomplete staining; 2, staining; >1 cm circumferentially). Intracranial and intraconal spread of dye was evaluated...
Source: Comparative Medicine - February 20, 2024 Category: Zoology Authors: Stephanie A Hon Stephen Parry Jordyn M Boesch Cristina de Miguel Garcia Source Type: research
A Feasibility Study for a Novel trans-infraorbital Canal Approach to the Maxillary Nerve in Pigs ( < em > Sus domesticus < /em > )
This study evaluated the feasibility of a novel maxillary nerve block approach. In part I, cadavers were used to determine anatomic landmarks and assess maxillary nerve dye staining by using 0.03 mL kg-1 of a 1:10 mixture of commercial food dye and 0.5% bupivacaine. In part II, 10 additional pig cadavers underwent bilateral ultrasound-guided maxillary nerve blocks by using trans-infraorbital canal needle placement. The maxillary nerve was harvested and scored based on degree of staining (0 and 1, absent or incomplete staining; 2, staining; >1 cm circumferentially). Intracranial and intraconal spread of dye was evaluated...
Source: Comparative Medicine - February 20, 2024 Category: Zoology Authors: Stephanie A Hon Stephen Parry Jordyn M Boesch Cristina de Miguel Garcia Source Type: research
A Feasibility Study for a Novel trans-infraorbital Canal Approach to the Maxillary Nerve in Pigs ( < em > Sus domesticus < /em > )
This study evaluated the feasibility of a novel maxillary nerve block approach. In part I, cadavers were used to determine anatomic landmarks and assess maxillary nerve dye staining by using 0.03 mL kg-1 of a 1:10 mixture of commercial food dye and 0.5% bupivacaine. In part II, 10 additional pig cadavers underwent bilateral ultrasound-guided maxillary nerve blocks by using trans-infraorbital canal needle placement. The maxillary nerve was harvested and scored based on degree of staining (0 and 1, absent or incomplete staining; 2, staining; >1 cm circumferentially). Intracranial and intraconal spread of dye was evaluated...
Source: Comparative Medicine - February 20, 2024 Category: Zoology Authors: Stephanie A Hon Stephen Parry Jordyn M Boesch Cristina de Miguel Garcia Source Type: research
A Feasibility Study for a Novel trans-infraorbital Canal Approach to the Maxillary Nerve in Pigs ( < em > Sus domesticus < /em > )
This study evaluated the feasibility of a novel maxillary nerve block approach. In part I, cadavers were used to determine anatomic landmarks and assess maxillary nerve dye staining by using 0.03 mL kg-1 of a 1:10 mixture of commercial food dye and 0.5% bupivacaine. In part II, 10 additional pig cadavers underwent bilateral ultrasound-guided maxillary nerve blocks by using trans-infraorbital canal needle placement. The maxillary nerve was harvested and scored based on degree of staining (0 and 1, absent or incomplete staining; 2, staining; >1 cm circumferentially). Intracranial and intraconal spread of dye was evaluated...
Source: Comparative Medicine - February 20, 2024 Category: Zoology Authors: Stephanie A Hon Stephen Parry Jordyn M Boesch Cristina de Miguel Garcia Source Type: research
A Feasibility Study for a Novel trans-infraorbital Canal Approach to the Maxillary Nerve in Pigs ( < em > Sus domesticus < /em > )
This study evaluated the feasibility of a novel maxillary nerve block approach. In part I, cadavers were used to determine anatomic landmarks and assess maxillary nerve dye staining by using 0.03 mL kg-1 of a 1:10 mixture of commercial food dye and 0.5% bupivacaine. In part II, 10 additional pig cadavers underwent bilateral ultrasound-guided maxillary nerve blocks by using trans-infraorbital canal needle placement. The maxillary nerve was harvested and scored based on degree of staining (0 and 1, absent or incomplete staining; 2, staining; >1 cm circumferentially). Intracranial and intraconal spread of dye was evaluated...
Source: Comparative Medicine - February 20, 2024 Category: Zoology Authors: Stephanie A Hon Stephen Parry Jordyn M Boesch Cristina de Miguel Garcia Source Type: research
High-pressure Hydrodynamic Injection as a Method of Establishing Hepatitis B Virus Infection in Mice
Comp Med. 2024 Feb 16. doi: 10.30802/AALAS-CM-23-000050. Online ahead of print.ABSTRACTAmong several existing mouse models for hepatitis B virus (HBV) infection, the high-pressure hydrodynamic injection (HDI) method is frequently used in HBV research due to its economic advantages and ease of implementation. The use of the HDI method is influenced by factors such as mouse genetic background, age, sex, and the type of HBV plasmid used. This overview provides a multidimensional analysis and comparison of various factors that influence the effectiveness of the HBV mouse model established through HDI. The goal is to provide a ...
Source: Comparative Medicine - February 16, 2024 Category: Zoology Authors: Juan Wen Guoli Chen Tianshun Wang Wan Yu Zhengyun Liu Huan Wang Source Type: research
High-pressure Hydrodynamic Injection as a Method of Establishing Hepatitis B Virus Infection in Mice
Comp Med. 2024 Feb 16. doi: 10.30802/AALAS-CM-23-000050. Online ahead of print.ABSTRACTAmong several existing mouse models for hepatitis B virus (HBV) infection, the high-pressure hydrodynamic injection (HDI) method is frequently used in HBV research due to its economic advantages and ease of implementation. The use of the HDI method is influenced by factors such as mouse genetic background, age, sex, and the type of HBV plasmid used. This overview provides a multidimensional analysis and comparison of various factors that influence the effectiveness of the HBV mouse model established through HDI. The goal is to provide a ...
Source: Comparative Medicine - February 16, 2024 Category: Zoology Authors: Juan Wen Guoli Chen Tianshun Wang Wan Yu Zhengyun Liu Huan Wang Source Type: research
High-pressure Hydrodynamic Injection as a Method of Establishing Hepatitis B Virus Infection in Mice
Comp Med. 2024 Feb 16. doi: 10.30802/AALAS-CM-23-000050. Online ahead of print.ABSTRACTAmong several existing mouse models for hepatitis B virus (HBV) infection, the high-pressure hydrodynamic injection (HDI) method is frequently used in HBV research due to its economic advantages and ease of implementation. The use of the HDI method is influenced by factors such as mouse genetic background, age, sex, and the type of HBV plasmid used. This overview provides a multidimensional analysis and comparison of various factors that influence the effectiveness of the HBV mouse model established through HDI. The goal is to provide a ...
Source: Comparative Medicine - February 16, 2024 Category: Zoology Authors: Juan Wen Guoli Chen Tianshun Wang Wan Yu Zhengyun Liu Huan Wang Source Type: research
High-pressure Hydrodynamic Injection as a Method of Establishing Hepatitis B Virus Infection in Mice
Comp Med. 2024 Feb 16. doi: 10.30802/AALAS-CM-23-000050. Online ahead of print.ABSTRACTAmong several existing mouse models for hepatitis B virus (HBV) infection, the high-pressure hydrodynamic injection (HDI) method is frequently used in HBV research due to its economic advantages and ease of implementation. The use of the HDI method is influenced by factors such as mouse genetic background, age, sex, and the type of HBV plasmid used. This overview provides a multidimensional analysis and comparison of various factors that influence the effectiveness of the HBV mouse model established through HDI. The goal is to provide a ...
Source: Comparative Medicine - February 16, 2024 Category: Zoology Authors: Juan Wen Guoli Chen Tianshun Wang Wan Yu Zhengyun Liu Huan Wang Source Type: research
Pigs in Transplantation Research and Their Potential as Sources of Organs in Clinical Xenotransplantation
Comp Med. 2024 Feb 15. doi: 10.30802/AALAS-CM-23-000030. Online ahead of print.ABSTRACTThe pig has long been used as a research animal and has now gained importance as a potential source of organs for clinical xenotransplantation. When an organ from a wild-type (i.e., genetically unmodified) pig is transplanted into an immunosuppressed nonhuman primate, a vigorous host immune response causes hyperacute rejection (within minutes or hours). This response has been largely overcome by 1) extensive gene editing of the organ-source pig and 2) the administration to the recipient of novel immunosuppressive therapy based on blockad...
Source: Comparative Medicine - February 15, 2024 Category: Zoology Authors: Sikandar S Raza Hidetaka Hara Willard Eyestone David Ayares David C Cleveland David K C Cooper Source Type: research
Pigs in Transplantation Research and Their Potential as Sources of Organs in Clinical Xenotransplantation
Comp Med. 2024 Feb 15. doi: 10.30802/AALAS-CM-23-000030. Online ahead of print.ABSTRACTThe pig has long been used as a research animal and has now gained importance as a potential source of organs for clinical xenotransplantation. When an organ from a wild-type (i.e., genetically unmodified) pig is transplanted into an immunosuppressed nonhuman primate, a vigorous host immune response causes hyperacute rejection (within minutes or hours). This response has been largely overcome by 1) extensive gene editing of the organ-source pig and 2) the administration to the recipient of novel immunosuppressive therapy based on blockad...
Source: Comparative Medicine - February 15, 2024 Category: Zoology Authors: Sikandar S Raza Hidetaka Hara Willard Eyestone David Ayares David C Cleveland David K C Cooper Source Type: research
A Novel Scoring System for Humane Endpoints in Mice with Cecal Ligation and Puncture-Induced Sepsis
The objective of this study was to establish a scoring system for mice undergoing cecal ligation and puncture (CLP)-induced sepsis based on 3 visual parameters: respiratory status, activity and response to stimulus (ASR), and eye appearance, with scores ranging from 0 to 3. In the first study, we evaluated interobserver agreement. Veterinary and investigative staff assessed 283 mice with CLP and had substantial to near-perfect agreement for all 3 parameters as evaluated using weighted Cohen κ statistic. The second study assessed the ability of the scoring system and temperature to predict death. The scoring system and sub...
Source: Comparative Medicine - January 12, 2024 Category: Zoology Authors: Lindsey T Ferguson Ammar A Rashied Zhe Liang Tetsuya Yumoto Jerome C Anyalebechi David A Swift Marina S Hernandes Robert T Krafty Craig M Coopersmith Vanessa K Lee Source Type: research
Spontaneous Pituitary Neoplasm in Two Female Geriatric Southern Giant Pouched Rats ( < em > Cricetomys ansorgei < /em > )
Comp Med. 2023 Dec 1;73(6):486-493. doi: 10.30802/AALAS-CM-23-000051.ABSTRACTSouthern giant pouched rats (Cricetomys ansorgei) are a small muroid species native to the sub-Saharan Africa. Their exceptionally developed olfactory system, trainability, and relatively small size makes them useful working animals for various applications in humanitarian work. At our institution, a breeding colony of Southern giant pouched rats is maintained to study their physiology and utility as scent detectors. This case report describes the occurrence of spontaneous pituitary neoplasms with distinct clinical presentations in 2 geriatric (ap...
Source: Comparative Medicine - January 12, 2024 Category: Zoology Authors: Anna L Voigt Sophie Nelissen Aaron J Percival Henning U Voss Elizabeth S Lavin Andrew D Miller Erica R Feldman Source Type: research
Pharmacokinetics of a Single Transdermal Dose of Mirtazapine in Rhesus Macaques ( < em > Macaca mulatta < /em > )
Comp Med. 2023 Dec 1;73(6):432-438. doi: 10.30802/AALAS-CM-23-000060.ABSTRACTDecreased appetite is a common clinical problem in captive rhesus macaques (Macaca mulatta). Mirtazapine, a tetracyclic antidepressant originally developed for humans, has shown promise as a safe and effective promoter of weight gain and appetite in several veterinary species including rhesus and cynomolgus macaques. Although mirtazapine is available as oral formulations, transdermal delivery in macaques with reduced appetite would allow quick, painless, topical application. Here we describe the pharmacokinetics of a single application of a widely...
Source: Comparative Medicine - January 12, 2024 Category: Zoology Authors: David W Bissinger Luke A Wittenburg Laura M Garzel Diane E Stockinger Gregory B Timmel Source Type: research
A Review of the Effects of Some Extrinsic Factors on Mice Used in Research
Comp Med. 2023 Dec 1;73(6):413-431. doi: 10.30802/AALAS-CM-23-000028.ABSTRACTAnimals have been used in research for over 2,000 y. From very crude experiments conducted by ancient scholars, animal research, as a science, was refined over hundreds of years to what we know it as today. However, the housing conditions of animals used for research did not improve significantly until less than 100 years ago when guidelines for housing research animals were first published. In addition, it was not until relatively recently that some extrinsic factors were recognized as a research variable, even when animals were housed under reco...
Source: Comparative Medicine - January 12, 2024 Category: Zoology Authors: Alfonso S Gozalo William R Elkins Source Type: research
