Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research
Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research
Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research
Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research
Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research
Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research
Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research
Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research
Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research
Assessment of Risperidone Toxicity in Zebrafish (Danio rerio) Embryos
Comp Med. 2023 Aug 3. doi: 10.30802/AALAS-CM-22-000123. Online ahead of print.ABSTRACTRisperidone is an antipsychotic medication used in the treatment of conditions like autism and schizophrenia. The goalof the current study was to examine the effects of risperidone in zebrafish embryos (Danio rerio) with regard to survival, development, and cardiac and neural systems. The results showed that concentrations above 100 μM were associated with deaths, teratogenic effects, and cardiotoxic and neurotoxic effects. The findings support the utility of zebrafish for toxicological screening studies.PMID:37536933 | DOI:10.30802/AALA...
Source: Comparative Medicine - August 3, 2023 Category: Zoology Authors: Hirla Costa Silva Fukushima Ricardo Lacava Bailone Ricardo Carneiro Borra Source Type: research
Assessment of Risperidone Toxicity in Zebrafish (Danio rerio) Embryos
Comp Med. 2023 Aug 3. doi: 10.30802/AALAS-CM-22-000123. Online ahead of print.ABSTRACTRisperidone is an antipsychotic medication used in the treatment of conditions like autism and schizophrenia. The goalof the current study was to examine the effects of risperidone in zebrafish embryos (Danio rerio) with regard to survival, development, and cardiac and neural systems. The results showed that concentrations above 100 μM were associated with deaths, teratogenic effects, and cardiotoxic and neurotoxic effects. The findings support the utility of zebrafish for toxicological screening studies.PMID:37536933 | DOI:10.30802/AALA...
Source: Comparative Medicine - August 3, 2023 Category: Zoology Authors: Hirla Costa Silva Fukushima Ricardo Lacava Bailone Ricardo Carneiro Borra Source Type: research
Assessment of Risperidone Toxicity in Zebrafish (Danio rerio) Embryos
Comp Med. 2023 Aug 3. doi: 10.30802/AALAS-CM-22-000123. Online ahead of print.ABSTRACTRisperidone is an antipsychotic medication used in the treatment of conditions like autism and schizophrenia. The goalof the current study was to examine the effects of risperidone in zebrafish embryos (Danio rerio) with regard to survival, development, and cardiac and neural systems. The results showed that concentrations above 100 μM were associated with deaths, teratogenic effects, and cardiotoxic and neurotoxic effects. The findings support the utility of zebrafish for toxicological screening studies.PMID:37536933 | DOI:10.30802/AALA...
Source: Comparative Medicine - August 3, 2023 Category: Zoology Authors: Hirla Costa Silva Fukushima Ricardo Lacava Bailone Ricardo Carneiro Borra Source Type: research
Assessment of Risperidone Toxicity in Zebrafish (Danio rerio) Embryos
Comp Med. 2023 Aug 3. doi: 10.30802/AALAS-CM-22-000123. Online ahead of print.ABSTRACTRisperidone is an antipsychotic medication used in the treatment of conditions like autism and schizophrenia. The goalof the current study was to examine the effects of risperidone in zebrafish embryos (Danio rerio) with regard to survival, development, and cardiac and neural systems. The results showed that concentrations above 100 μM were associated with deaths, teratogenic effects, and cardiotoxic and neurotoxic effects. The findings support the utility of zebrafish for toxicological screening studies.PMID:37536933 | DOI:10.30802/AALA...
Source: Comparative Medicine - August 3, 2023 Category: Zoology Authors: Hirla Costa Silva Fukushima Ricardo Lacava Bailone Ricardo Carneiro Borra Source Type: research
Mitigation of Fibrosis after Myocardial Infarction in Rats by Using a Porcine Cholecyst Extracellular Matrix
In this study, we used an epicardial graft prepared of porcine cholecystic extracellular matrix to treat experimental nonfatal MI in rats. Graft-assisted healing was characterized by reduced fibrosis, with scanty deposition of type I collagen. Histologically, the tissue response was associated with a favorable regenerative reaction predominated by CD4-positive helper T lymphocytes, enhanced angiogenesis, and infiltration of proliferating cells. These observations indicate that porcine cholecystic extracellular matrix delayed the fibrotic reactionand support its use as a potential biomaterial for mitigating fibrosis after M...
Source: Comparative Medicine - August 1, 2023 Category: Zoology Authors: Reshma S Nair Praveen K Soghan Sachin J Shenoy Mukund A Prabhu Vikas Kumar Surya Ramachandran Thapasimuthu V Anilkumar Source Type: research
Mitigation of Fibrosis after Myocardial Infarction in Rats by Using a Porcine Cholecyst Extracellular Matrix
In this study, we used an epicardial graft prepared of porcine cholecystic extracellular matrix to treat experimental nonfatal MI in rats. Graft-assisted healing was characterized by reduced fibrosis, with scanty deposition of type I collagen. Histologically, the tissue response was associated with a favorable regenerative reaction predominated by CD4-positive helper T lymphocytes, enhanced angiogenesis, and infiltration of proliferating cells. These observations indicate that porcine cholecystic extracellular matrix delayed the fibrotic reactionand support its use as a potential biomaterial for mitigating fibrosis after M...
Source: Comparative Medicine - August 1, 2023 Category: Zoology Authors: Reshma S Nair Praveen K Soghan Sachin J Shenoy Mukund A Prabhu Vikas Kumar Surya Ramachandran Thapasimuthu V Anilkumar Source Type: research
