Veterinary Management of Harderian Gland Tumors in Cancer Rainbow (crainbow) HER2-Positive Mice
We describe a range of interventions, including close monitoring, topical palliative care, and surgical bilateral enucleation. Based on our data and previous reports in the literature, the overexpression of HER2 in Harderian gland tissue and subsequent tumor formation likely was driven by MMTV-Cre expression in the Harderian gland.PMID:36744508 | PMC:PMC9827610 | DOI:10.30802/AALAS-CM-22-000061 (Source: Comparative Medicine)
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Angela Garner Joshua D Ginzel Joshua C Snyder Jeffrey I Everitt Chelsea D Landon Source Type: research
A Clinical Scoring Systems for the Evaluation of < em > Corynebacterium bovis < /em > -associated Disease in NSG Mice
Comp Med. 2022 Dec 1;72(6):386-393. doi: 10.30802/AALAS-CM-22-000098.ABSTRACTClinical signs of Corynebacterium bovis infections are well-known in athymic nude mice. However, C. bovis can also infect and cause clinical signs in many hirsute, immunocompromised mouse strains such as NSG (NOD. Cg-Prkdcscid Il2rgtm1Wgl/SzJ). Typically, the clinical assessment of C. bovis-infected mice begins when overt clinical signs are initially observed and thus the early course of infection has not been thoroughly described. The goal of this study was to characterize the clinical progression of C. bovis infection in NSG mice under experimen...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Christopher A Manuel Emily C Pearson Umarani Pugazhenthi Michael K Fink Lauren M Habenicht Derek L Fong Jori K Leszczynski Michael J Schurr Source Type: research
Comparing Variability in Measurement of Subcutaneous Tumors in Mice Using 3D Thermal Imaging and Calipers
Comp Med. 2022 Dec 1;72(6):364-375. doi: 10.30802/AALAS-CM-22-000033.ABSTRACTRepeatable tumor measurements are key to accurately assessing tumor growth and treatment efficacy. A preliminary study that we conducted showed that a novel 3D and thermal imaging system (3D-TI) for measuring subcutaneous tumors in rodents significantly reduced interoperator variability across 3 in vivo efficacy studies. Here we further studied this reduction in interoperator variability across a much larger dataset. A dataset consisting of 6,532 paired 3D-TI and caliper interoperator measurements was obtained from tumor scans and measurements in ...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Daniel W Brough Jake T Murkin Hope E Amos Andrew I Smith Karl D Turley Source Type: research
Melioidosis in Cynomolgus Macaques ( < em > Macaca Fascicularis < /em > ) Imported to the United States from Cambodia
Comp Med. 2022 Dec 1;72(6):394-402. doi: 10.30802/AALAS-CM-22-000024.ABSTRACTMelioidosis, a potentially fatal infectious disease of humans and animals, including nonhuman primates (NHPs), is caused by the high-consequence pathogen Burkholderia pseudomallei. This environmental bacterium is found in the soil and water of tropical regions, such as Southeast Asia, where melioidosis is endemic. The global movement of humans and animals can introduce B. pseudomallei into nonendemic regions of the United States, where environmental conditions could allow establishment of the organism. Approximately 60% of NHPs imported into the U...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Sara J Taetzsch Erin M Swaney Jay E Gee Pablo M Hidalgo Kelly R Broussard Roosecelis B Martines David D Blaney G Gale Galland Christopher A Gulvik Chung K Marston Lindy Liu Mindy G Elrod Marlene DeLeon-Carnes Ronald D Tyler William A Bower Julu Bhatnager Source Type: research
Infectivity and Shedding of Mouse Kidney Parvovirus After Oronasal Inoculation of C57BL/6, CD1, and NSG Mice
Comp Med. 2022 Dec 1;72(6):376-385. doi: 10.30802/AALAS-CM-22-000066.ABSTRACTMouse kidney parvovirus (MKPV), the etiology of murine inclusion body nephropathy, has been identified globally in mice used for research, with an estimated prevalence of 10% in academic colonies. In immunodeficient strains, MKPV causes significant morbidity and mortality, and severe renal pathology. In contrast, in immunocompetent mice, the infection is subclinical and causes minimal pathology. We investigated viral infectivity and shedding in inbred C57BL/6NCrl (B6), outbred Crl:CD1(ICR) (CD1), and highly immunocompromised NOD. Cg - Prkdc scid I...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Mandy L Kain Rodolfo Ricart J Arbona Kenneth S Henderson Rajeev Dhawan Sebastien Monette Neil S Lipman Source Type: research
Characterization and Prevention of Hypovitaminosis C in Chimeric Mice with Humanized Livers
Comp Med. 2022 Dec 1;72(6):355-363. doi: 10.30802/AALAS-CM-22-000027.ABSTRACTHumanized liver chimeric mice (PXB-mice) are generated by the transplantation of human hepatocytes into mice that have severe combined immunodeficiency and express an albumin-promoted urokinase-type plasminogen activator (cDNA-uPA/SCID) transgene. Human hepatocytes cannot synthesize ascorbic acid (AA; commonly called vitamin C) and humans require supplementation to prevent vitamin C deficiency. PXB-mouse livers contain up to approximately 95% human hepatocytes, which likely affects AA synthesis. To determine whether dietary AA supplementation prev...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Erin K Touchette Maria C Bates Mitch C Johnson Tracy C O'Brien Roger J Melton Kelly R Long Masakazu Kakuni Matthew Baginski Daniel R Radiloff John E Sagartz Source Type: research
Eliminating Potential Effects of Other Infections during Selection of Nonhuman Primates for COVID-19 Research
Comp Med. 2023 Jan 4;73(1):45-57. doi: 10.30802/AALAS-CM-21-000086. Online ahead of print.ABSTRACTThe study of nonhuman primates (NHP) can provide significant insights into our understanding numerous infectious agents. The etiological agent of COVID-19, SARS-CoV-2 virus, first emerged in 2019 and has so far been responsible for the deaths of over 4 million people globally. In the frenzied search to understand its pathogenesis and immunology and to find measures for prevention and control of this pandemic disease, NHP, particularly macaques, are the preferred model because they manifest similar clinical signs and immunologi...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: M árcia Cr Andrade B árbara Rp Lemos Larissa M Silva Jerilyn K Pecotte Source Type: research
Animal Models to Study Neurologic Manifestations of COVID-19
Comp Med. 2023 Jan 19;73(1):91-103. doi: 10.30802/AALAS-CM-22-000073. Online ahead of print.ABSTRACTSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the worldwide coronavirus (COVID-19) pandemic, has infected an estimated 525 million people with over 6 million deaths. Although COVID-19 is primarily a respiratory disease, an escalating number of neurologic symptoms have been reported in humans. Some neurologic symptoms, such as loss of smell or taste, are mild. However, other symptoms, such as meningoencephalitis or stroke, are potentially fatal. Along with surveys and postmortem evaluations on hum...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Kelsey C Carpenter Jibing Yang Jiajie Jessica Xu Source Type: research
Veterinary Management of Harderian Gland Tumors in Cancer Rainbow (crainbow) HER2-Positive Mice
We describe a range of interventions, including close monitoring, topical palliative care, and surgical bilateral enucleation. Based on our data and previous reports in the literature, the overexpression of HER2 in Harderian gland tissue and subsequent tumor formation likely was driven by MMTV-Cre expression in the Harderian gland.PMID:36744508 | PMC:PMC9827610 | DOI:10.30802/AALAS-CM-22-000061 (Source: Comparative Medicine)
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Angela Garner Joshua D Ginzel Joshua C Snyder Jeffrey I Everitt Chelsea D Landon Source Type: research
A Clinical Scoring Systems for the Evaluation of < em > Corynebacterium bovis < /em > -associated Disease in NSG Mice
Comp Med. 2022 Dec 1;72(6):386-393. doi: 10.30802/AALAS-CM-22-000098.ABSTRACTClinical signs of Corynebacterium bovis infections are well-known in athymic nude mice. However, C. bovis can also infect and cause clinical signs in many hirsute, immunocompromised mouse strains such as NSG (NOD. Cg-Prkdcscid Il2rgtm1Wgl/SzJ). Typically, the clinical assessment of C. bovis-infected mice begins when overt clinical signs are initially observed and thus the early course of infection has not been thoroughly described. The goal of this study was to characterize the clinical progression of C. bovis infection in NSG mice under experimen...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Christopher A Manuel Emily C Pearson Umarani Pugazhenthi Michael K Fink Lauren M Habenicht Derek L Fong Jori K Leszczynski Michael J Schurr Source Type: research
Comparing Variability in Measurement of Subcutaneous Tumors in Mice Using 3D Thermal Imaging and Calipers
Comp Med. 2022 Dec 1;72(6):364-375. doi: 10.30802/AALAS-CM-22-000033.ABSTRACTRepeatable tumor measurements are key to accurately assessing tumor growth and treatment efficacy. A preliminary study that we conducted showed that a novel 3D and thermal imaging system (3D-TI) for measuring subcutaneous tumors in rodents significantly reduced interoperator variability across 3 in vivo efficacy studies. Here we further studied this reduction in interoperator variability across a much larger dataset. A dataset consisting of 6,532 paired 3D-TI and caliper interoperator measurements was obtained from tumor scans and measurements in ...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Daniel W Brough Jake T Murkin Hope E Amos Andrew I Smith Karl D Turley Source Type: research
Melioidosis in Cynomolgus Macaques ( < em > Macaca Fascicularis < /em > ) Imported to the United States from Cambodia
Comp Med. 2022 Dec 1;72(6):394-402. doi: 10.30802/AALAS-CM-22-000024.ABSTRACTMelioidosis, a potentially fatal infectious disease of humans and animals, including nonhuman primates (NHPs), is caused by the high-consequence pathogen Burkholderia pseudomallei. This environmental bacterium is found in the soil and water of tropical regions, such as Southeast Asia, where melioidosis is endemic. The global movement of humans and animals can introduce B. pseudomallei into nonendemic regions of the United States, where environmental conditions could allow establishment of the organism. Approximately 60% of NHPs imported into the U...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Sara J Taetzsch Erin M Swaney Jay E Gee Pablo M Hidalgo Kelly R Broussard Roosecelis B Martines David D Blaney G Gale Galland Christopher A Gulvik Chung K Marston Lindy Liu Mindy G Elrod Marlene DeLeon-Carnes Ronald D Tyler William A Bower Julu Bhatnager Source Type: research
Infectivity and Shedding of Mouse Kidney Parvovirus After Oronasal Inoculation of C57BL/6, CD1, and NSG Mice
Comp Med. 2022 Dec 1;72(6):376-385. doi: 10.30802/AALAS-CM-22-000066.ABSTRACTMouse kidney parvovirus (MKPV), the etiology of murine inclusion body nephropathy, has been identified globally in mice used for research, with an estimated prevalence of 10% in academic colonies. In immunodeficient strains, MKPV causes significant morbidity and mortality, and severe renal pathology. In contrast, in immunocompetent mice, the infection is subclinical and causes minimal pathology. We investigated viral infectivity and shedding in inbred C57BL/6NCrl (B6), outbred Crl:CD1(ICR) (CD1), and highly immunocompromised NOD. Cg - Prkdc scid I...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Mandy L Kain Rodolfo Ricart J Arbona Kenneth S Henderson Rajeev Dhawan Sebastien Monette Neil S Lipman Source Type: research
Characterization and Prevention of Hypovitaminosis C in Chimeric Mice with Humanized Livers
Comp Med. 2022 Dec 1;72(6):355-363. doi: 10.30802/AALAS-CM-22-000027.ABSTRACTHumanized liver chimeric mice (PXB-mice) are generated by the transplantation of human hepatocytes into mice that have severe combined immunodeficiency and express an albumin-promoted urokinase-type plasminogen activator (cDNA-uPA/SCID) transgene. Human hepatocytes cannot synthesize ascorbic acid (AA; commonly called vitamin C) and humans require supplementation to prevent vitamin C deficiency. PXB-mouse livers contain up to approximately 95% human hepatocytes, which likely affects AA synthesis. To determine whether dietary AA supplementation prev...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: Erin K Touchette Maria C Bates Mitch C Johnson Tracy C O'Brien Roger J Melton Kelly R Long Masakazu Kakuni Matthew Baginski Daniel R Radiloff John E Sagartz Source Type: research
Eliminating Potential Effects of Other Infections during Selection of Nonhuman Primates for COVID-19 Research
Comp Med. 2023 Jan 4;73(1):45-57. doi: 10.30802/AALAS-CM-21-000086. Online ahead of print.ABSTRACTThe study of nonhuman primates (NHP) can provide significant insights into our understanding numerous infectious agents. The etiological agent of COVID-19, SARS-CoV-2 virus, first emerged in 2019 and has so far been responsible for the deaths of over 4 million people globally. In the frenzied search to understand its pathogenesis and immunology and to find measures for prevention and control of this pandemic disease, NHP, particularly macaques, are the preferred model because they manifest similar clinical signs and immunologi...
Source: Comparative Medicine - February 6, 2023 Category: Zoology Authors: M árcia Cr Andrade B árbara Rp Lemos Larissa M Silva Jerilyn K Pecotte Source Type: research
