Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research

Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research

Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research

Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research

Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research

Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research

Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research

Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research

Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research

Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research

Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research

Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research

Evaluation of Uveitis Induced in Rats by a Type I Collagen Peptide as a Model for Childhood Arthritis-associated Uveitis
In this study, we evaluated MAA-induced uveitis in rats as a potential model for JIA-uveitis. We characterizedMAA-induced uveitis by assessing its relationship to age and sex; tracking the occurrence of arthritis, enthesitis, and ANApositivity; and measuring vitreous fluid inflammatory biomarkers. Juvenile and adult and male and female Lewis rats (Rattusnorvegicus) were inoculated with MAA. Slit-lamp biomicroscopy, indirect ophthalmoscopy, and joint examinations wereperformed 3 times weekly. Rats were euthanized at 4 wk after MAA inoculation, and plasma ANA testing, vitreous inflammatorybiomarker assays, and globe histopat...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Stephanie C Osinchuk Bruce H Grahn Tracy D Wilson Brooke N Thompson David A Hart Kim D Harrison David Ml Cooper Arash Panahifar Alan M Rosenberg Source Type: research

Seasonal Variation of Laboratory Animals as a Consideration for Research Reproducibility
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000033. Online ahead of print.ABSTRACTLaboratory rodents are generally maintained under standardized conditions in order to control the effects of extrinsic factorson research. However, despite attempts to standardize conditions, variability can nonetheless confound efforts directed towardresearch reproducibility. Here we explore some of the existing literature on the potential impact of seasonal variability as anextrinsic factor that can potentially impact research results. We discuss the influence of seasonal changes in association withan internal clock mechanism that might...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Mark A Suckow No é Tirado-Muñiz Source Type: research

Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Tupaia belangeri)
Comp Med. 2023 Aug 7. doi: 10.30802/AALAS-CM-23-000043. Online ahead of print.ABSTRACTTree shrews display obvious reproductive cycles, and sexually mature male tree shrews produce little or no sperm withextremely low motility during the nonreproductive season; the mechanism underlying this phenomenon remains unknown.Because testis-specific serine/threonine kinases (TSSK) are specifically expressed in the testis and male germ cells of mammals,we hypothesized that they may have an important role in spermatogenesis or sperm function regulation in tree shrews.In addition, the expression, distribution, subcellular localization,...
Source: Comparative Medicine - August 7, 2023 Category: Zoology Authors: Xia Tan Xin Zhang Xiang Li Minghua Yang Source Type: research