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Daily thermal variability does not modify long-term gene expression relative to stable thermal environments: A case study of a tropical fish
Comp Biochem Physiol A Mol Integr Physiol. 2023 Oct 8:111532. doi: 10.1016/j.cbpa.2023.111532. Online ahead of print.ABSTRACTGlobal warming is leading to an increase in the frequency and intensity of extreme weather events, magnifying the breadth of temperatures faced by ectotherms across days and seasons. Despite the importance and ecological relevance of diurnal thermal variability, the vast majority of knowledge on gene expression patterns and physiology stems from animals acclimated to constant temperatures or in the early stages of exposure to a new temperature regime. If heterothermal environments modulate responses ...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - October 10, 2023 Category: Physiology Authors: Hanna Scheuffele Erica V Todd John A Donald Timothy D Clark Source Type: research

Molecular cloning and tissue distribution of glucokinase and glucose-6-phosphatase catalytic subunit paralogs in largemouth bass Micropterus salmoides: Regulation by dietary starch levels and a glucose load
In this study, the full length cDNA sequences of genes encoding glucokinase (Gck, catalyzing glucose phosphorylation) and glucose-6-phosphatase catalytic subunit (G6pc, catalyzing glucose dephosphorylation) were cloned by the RACE method from the liver of LMB. Subsequently, the distribution of g6pc and gck as well as their transcriptional regulation by dietary starch levels and a glucose load were investigated. Only one gck gene was identified, while the tandem duplication of g6pca.1 gene was named as g6pca.2 in LMB. The full cDNA sequences of g6pca.1, g6pca.2 and gck in LMB were 1585, 1813 and 2115 bp in length, encoding ...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - October 6, 2023 Category: Physiology Authors: Ru Xia Hong-Kang Liu Xi-Feng Liu Xin Deng Chuan-Jie Qin Yuan-Fa He Shi-Mei Lin Yong-Jun Chen Source Type: research

HoTDAM! An easy-to-use automated assay expands the inducible thermotolerance phenotype in Drosophila melanogaster: Heat hardening reduces motility
In this study, the locomotion response of Drosophila melanogaster to relatively high temperature (39 and 40.5 °C) was quantified using the TriKinetics Drosophila Activity Monitor (DAM2 system). The absence of locomotion was defined as the state of physiological collapse resulting from extended exposure to high temperature. An easy-to-use executable application that allows the user to automatically extract individual TKD from the activity data was developed. For validation, manual TKD assays were performed in parallel to automated assays across multiple factors, including sex, hardening, recovery time after hardening, and ...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - September 24, 2023 Category: Physiology Authors: Blase Rokusek Sunayn Cheku Matthew Rokusek Christopher J Waples Lawrence Harshman Kimberly A Carlson Source Type: research