Bioprinting of Glioma Stem Cells Improves Their Endotheliogenic Potential
In this study, the viability of cells after bioprinting was 86.27 ± 2.41%. Furthermore, compared with traditional suspension culture, the proliferation of 3D printed GSCs was more stable. Through the transmission electron microscopy (TEM), numerous long microvilli of cells cultured in 3D bioprinted scaffolds were observed. 3D bioprinted GSCs also have more abundant mitochondria and rough endoplasmic reticulum. Additionally, the stemness properties, the expression of tumor angiogenesis-related genes and vascularization potential of 3D bioprinted GSCs in vitro were higher than that of suspension cultured...
Source: Colloids and Surfaces B: Biointerfaces - August 8, 2018 Category: Biochemistry Source Type: research

Electrostatic and hydrophobic controlled self-assembly of PDMS-E grafted gelatin for self-cleaning application
Publication date: Available online 7 August 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zhen Zhang, Zhaosheng Hou, Congde Qiao, Cong Zhu, Kejun Zhou, Xinhai Xu, Tianduo Li, Jing XuAbstractUnderstanding the assembly mechanisms of supramolecular architectures in nature is essential for the design and synthesis of novel biomaterials. In the work, self–assembly of gelatin–mono epoxy terminated polydimethylsiloxane polymer (PGG) controlled by electrostatic and hydrophobic interactions between gelatin and sodium dodecyl sulfate (SDS) was investigated in suit. Confocal laser scanning microscopy, circu...
Source: Colloids and Surfaces B: Biointerfaces - August 8, 2018 Category: Biochemistry Source Type: research

Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y
In this study, we show that hydroperoxidation may be photoinduced when eosin Y is incorporated into Langmuir monolayers that serve as cell membrane models. This occurs for Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which have unsaturation in their hydrophobic chains. In contrast, light irradiation had no effect on monolayers of saturated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Evidence of hydroperoxidation was obtained from the area increase in eosin-containing DOPC and POPC monolayers upon irradiation, which was accompanie...
Source: Colloids and Surfaces B: Biointerfaces - August 6, 2018 Category: Biochemistry Source Type: research

Fabrication of Dihydroxyflavone-conjugated Hyaluronic Acid Nanogels for Targeted Antitumoral Effect
Publication date: Available online 6 August 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yu Ri Choi, Hyun-Jong Kim, Guk Young Ahn, Min Jeong Lee, Ju Ri Park, Dae-Ryong Jun, Tae-Kyoung Ryu, Joo Woong Park, Eunji Shin, Sung-Wook ChoiAbstractWe prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear ma...
Source: Colloids and Surfaces B: Biointerfaces - August 6, 2018 Category: Biochemistry Source Type: research

Linalool Bioactive Properties and Potential Applicability in Drug Delivery Systems
Publication date: Available online 3 August 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Irina Pereira, Patrícia Severino, Ana C. Santos, Amélia M. Silva, Eliana B. SoutoAbstractThe medicinal properties of essential oils from aromatic plants are known since antiquity. Currently, the technological innovation enabled the reinvention of the ancient plant knowledge leading to the identification and extraction of organic compounds present in essential oils. These organic compounds belong mainly to the terpene group and are accountable for the wide range of bioactive properties attributed to essenti...
Source: Colloids and Surfaces B: Biointerfaces - August 4, 2018 Category: Biochemistry Source Type: research

The characteristics of a preservative-free contact lens care solution on lysozyme adsorption and interfacial friction behavior
Publication date: Available online 3 August 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Chen-Ying Su, Chi-Chun Lai, Lung-Kun Yeh, Kuan-Yi Li, Bo-Wu Shih, Ching-Li Tseng, Hsu-Wei FangAbstractThe population of soft contact lens wearers is increasing, thus the issues of feeling comfortable and contact lens-induced clinical symptoms are concerned. Both lysozyme deposition and mechanical friction among contact lens and the eye can induce discomfort and eye conditions. Therefore, we characterized our developed preservative-free contact lens care solution on lysozyme adsorption and lubrication. Two distinct lens ...
Source: Colloids and Surfaces B: Biointerfaces - August 4, 2018 Category: Biochemistry Source Type: research

Synthesis, characterization and energy transfer studies of fluorescent dye-labeled metal-chelating polymers anchoring pendant thiol groups for surface modification of quantum dots and investigation on their application for pH-responsive controlled release of doxorubicin
We describe the synthesis of an end-functionalized fluorescent dye-labeled poly(N-(2-thiolethyl methacrylamide) as a metal chelating polymer with a very narrow size distribution and controllable molecular weights by reversible addition–fragmentation chain transfer polymerization. In following, we apply this water-soluble metal-chelating polymer for surface modification of cadmium telluride quantum dots (CdTe QDs) by ligand exchange method. The obtained poly(N-(2-thiolethyl methacrylamide)/CdTe QDs hybrid was fully characterized using Fourier transform infrared spectroscopy (FT-IR), thermo-gravimetric analysis (TG) an...
Source: Colloids and Surfaces B: Biointerfaces - August 3, 2018 Category: Biochemistry Source Type: research

Role of zein incorporation on hydrophobic drug-loading capacity and colloidal stability of phospholipid nanoparticles
Publication date: Available online 2 August 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Soon-Seok Hong, Raj Kumar Thapa, Jin-Hee Kim, Soo-Yeon Kim, Jong Oh Kim, Jin-Ki Kim, Han-Gon Choi, Soo-Jeong LimAbstractLiposome, phosphatidylcholine nanoparticle (PC-NP), is an attractive colloidal carrier of hydrophobic drugs but its clinical development is often limited by low drug-loading capacity and the physical instability. Zein is a water-insoluble amphiphilic protein obtained from the corn. We herein investigated a possibility to develop zein-phosphatidylcholine hybrid nanoparticle (Z/PC-NP) as an advanced hydr...
Source: Colloids and Surfaces B: Biointerfaces - August 2, 2018 Category: Biochemistry Source Type: research

Real-Time QCM-D monitoring of endothelial cells and macrophages adhering and spreading to SEMA4D/heparin surfaces
This study suggests that QCM-D combined with other appropriate methods can be utilized to explore the mechanisms for cellular interaction with biomaterial surfaces.Graphical AbstractThe adhering and spreading of endothelia cells (ECs) and macrophages (MAs) to SEMA4D/Heparin modified surface. (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - August 2, 2018 Category: Biochemistry Source Type: research

Immunotoxicity assessment of ordered mesoporous carbon nanoparticles modified with PVP/PEG
In conclusion, the ordered mesoporous carbon nanoparticles superficially modified by PVP or PEG perform well in immunological biocompatibility, and are likely to be a promising candidate as medicine carrier in pharmaceutics and clinic.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - August 1, 2018 Category: Biochemistry Source Type: research

Interaction of calmodulin with poly(acrylic acid) brushes: effects of high pressure, pH-value and ligand binding
Publication date: Available online 31 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Artem Levin, Claus CzeslikAbstractPoly(acrylic acid) (PAA) brushes are well-known to interact with proteins in an ionic strength-dependent way. Moreover, they provide a native-like environment that largely maintains the secondary structure and biological activity of adsorbed proteins. Recently, it has been shown that the application of high pressure can lead to a reduced protein adsorption at a PAA brush in the case of a positively charged protein. Here, we analyze the effect of pressure on the interactions between a pro...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

A Rational and Iterative Process for Targeted Nanoparticle Design and Validation
Publication date: Available online 31 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Laura Rodriguez-Lorenzo, Sarah D. Rafiee, Corine Reis, Ana Milosevic, Thomas L. Moore, Sandor Balog, Barbara Rothen-Rutishauser, Curzio Ruegg, Alke Petri-FinkABSTRACTThe lack of understanding of fundamental nano-bio interactions, and difficulties in designing particles stable in complex biological environments are major limitations to their translation into biomedical clinical applications. Here we present a multi-parametric approach to fully characterizing targeted nanoparticles, and emphasizes the significant effect th...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

A robust pH-sensitive unimolecular dendritic nanocarrier that enables targeted anti-cancer drug delivery via GLUT transporters
This study explores the potential of dendritic unimolecular nanoconstruct, PAMAM-Tryptophan-(N-acetylglucosamine) [PTN] as anti-cancer drug delivery system. The PAMAM dendrimers were modified with L-tryptophan and N-acetyl glucosamine (NAG) for higher drug loading and to utilize GLUT transporters, respectively. The nanocarriers were characterized by 1H NMR, DSC, and dynamic light scattering. Effect of doxorubicin (DOX)- loaded PTN was studied on MDA-MB-231 and HepG2 cells by cell viability assay. Further flow cytometry analysis was carried out to assess apoptosis. Pre-treatment with NAG was carried out to keep GLUT transpo...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

Shear stress regulated uptake of liposome-decorated microgels coated with a poly(dopamine) shell
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Maria Jose York-Duran, Pramod Kumar Ek, Maria Godoy-Gallardo, Leticia Hosta-RigauAbstractAdvanced multicompartment drug delivery platforms ensure the co-localization of several drugs within the same carrier, thus making it possible to achieve a more effective and safe therapeutic outcome. Herein, we report a novel multicompartment architecture by combining two intrinsically different systems, i.e., polymeric microgels and liposomes, with the aim to achieve different release kinetics for model compounds. We assemble poly(N-...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

Synergistic effects of titania nanotubes and silicon to enhance the osteogenic activity
In this study, titania nanotubes (TNTs) incorporating silicon (Si) were formed on Ti disks using anodization and electron beam evaporation (EBE) technology to improve the osteogenic activity. The amount of Si was exquisitely adjusted by controlling the duration of EBE to optimize the biofunctionality. As the Si was incorporated, the samples exhibited hydrophilic surfaces. Long lasting and controllable Si release was observed from the EBE-modified samples without cytotoxicity. Moreover, initial cell adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells were evaluated. The results showed a notab...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

Solid lipid nanoparticles optimized by 22 factorial design for skin administration: Cytotoxicity in NIH3T3 fibroblasts
Publication date: Available online 30 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Roberta Balansin Rigon, Maíra Lima Gonçalez, Patrícia Severino, Danilo Antonini Alves, Maria H.A. Santana, Eliana B. Souto, Marlus ChorilliAbstractThe present study focuses on the characterization of the cytotoxic profile on NIH3T3 mouse embryonic fibroblasts of solid lipid nanoparticles (SLN) optimized by a 22 full factorial design for skin administration. To build up the surface response charts, a design of experiments (DoE) based on 2 independent variables was used to obtain an optimized formulati...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

Improving Dispersive Property, Biocompatibility and Targeting Gene Transfection of Graphene Oxide by Covalent Attachment of Polyamidoamine Dendrimer and Glycyrrhetinic Acid
Publication date: Available online 30 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Feiyan Liu, Dan Yang, Yawen Liu, Qianqian Cao, Yulian Sun, Qiong Wang, Hao TangAbstractThe surface functional groups of GO have significant effects on the performances of GO-based gene delivery vector. In this work, the polyamidoamine (PAMAM) dendrimer and glycyrrhetinic acid (GA) were tethered onto the GO surface by one-step covalently cross-linking method. The micro-morphology, surface functional groups, and zeta potential of the obtained GO-PAMAM-GA hybrid were characterized and verified. The effects of GA amount in t...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

Preparation of self-assembled nanoparticles of ε-polylysine-sodium alginate: A sustained-release carrier for antigen delivery
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Jing Yuan, Lu Guo, Sijia Wang, Dan Liu, Xia Qin, Lili Zheng, Chunlian Tian, Xiaohu Han, Ran Chen, Ronghuan YinAbstractLow immunogenicity prohibits the widespread use of subunit vaccine against infectious diseases and cancers. Hence, a new generation of adjuvants and delivery systems is indispensable for more potent antigen-specific immune responses. Predominantly, nanoparticles formulated from biodegradable polymers are being widely explored as carriers of novel vaccines owing to their outstanding natural properties. We fa...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities
This study aimed to develop an environmentally friendly and cost-effective approach to synthesize green silver nanoparticles (AgNPs) from silver precursors. Green synthesis of AgNPs was accomplished using the aqueous extract of turmeric powder, in which plant biomaterials were used as a reducing as well as a capping agent. After 24 h of reaction, the yellow color of the extract was changed to dark brown-reddish due to the reduction of silver ions to AgNPs. AgNPs were characterized using UV–vis spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy (TEM), and energy dispersive X...
Source: Colloids and Surfaces B: Biointerfaces - July 31, 2018 Category: Biochemistry Source Type: research

Modification of gold surface by electrosynthesized mono aza crown ether substituted catechol-terminated alkane dithiol and its application as a new electrochemical sensor for trace detection of cadmium ions
In this study, an electroactive self-assembled monolayer (SAM) was developed by covalent attachment of a novel mono aza-crown ether substituted catechol-terminated hexane dithiol onto the gold surface. The electrochemical behavior of the fabricated SAM electrode was investigated using voltammetry techniques and electrochemical impedance spectroscopy (EIS). The obtained results from voltammetric experiments revealed that the crown ether moiety of SAM forms a selective complex with cadmium ion. Under optimal conditions, Cd2+ could be detected in the range of 15 µM to 65 µM with a detection limit of 4.5 µM. ...
Source: Colloids and Surfaces B: Biointerfaces - July 29, 2018 Category: Biochemistry Source Type: research

Construction of Ag-incorporated coating on Ti substrates for inhibited bacterial growth and enhanced osteoblast response
Publication date: Available online 29 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zhang Yuan, Peng Liu, Yansha Hao, Yao Ding, Kaiyong CaiAbstractIn orthopedic fields, effective anti-infection property and promotive biocompatibility on surface of titanium implants are two crucial factors for long-term successful implants. Herein, Ag nanoparticles (NPs) loaded TiO2 nanotubes (TNT) arrays were fabricated on Ti substrates with assistance of ultraviolet irradiation. Then, bioactive multilayer films of chitosan (CHI) and dialdehyde alginate (ADA) pair were deposited onto the Ag-loaded TNT arrays via a layer...
Source: Colloids and Surfaces B: Biointerfaces - July 29, 2018 Category: Biochemistry Source Type: research

Calcium phosphate particles stimulate exosome secretion from phagocytes for the enhancement of drug delivery
This study highlights the potential for the efficient production of exosomes using CaP particles for drug delivery.Graphical abstract (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - July 29, 2018 Category: Biochemistry Source Type: research

Lipids mediating the interaction of metronidazole with cell membrane models at the air-water interface
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Jefferson Carnevalle Rodrigues, Luciano CaseliAbstractIn this paper, metronidazole, a known compound with microbicide effect, was incorporated in Langmuir monolayers of selected phospholipids in order to investigate the interactions of this compound with cell membrane models by using tensiometric, spectroscopic and morphological techniques. Surface pressure-area isotherms showed that selected amounts of metronidazole condense DPPC monolayers, but expand DPPE monolayers. Vibrational spectroscopy pointed that metronidazole a...
Source: Colloids and Surfaces B: Biointerfaces - July 28, 2018 Category: Biochemistry Source Type: research

Mixed cationic liposomes for brain delivery of drugs by the intranasal route: The acetylcholinesterase reactivator 2-PAM as encapsulated drug model
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Tatiana N. Pashirova, Irina V. Zueva, Konstantin A. Petrov, Svetlana S. Lukashenko, Irek R. Nizameev, Natalya V. Kulik, Aleksandra D. Voloshina, Laszlo Almasy, Marsil K. Kadirov, Patrick Masson, Eliana B. Souto, Lucia Ya. Zakharova, Oleg G. SinyashinAbstractNew mixed cationic liposomes based on L-α-phosphatidylcholine and dihexadecylmethylhydroxyethylammonium bromide (DHDHAB) were designed to overcome the BBB crossing by using the intranasal route. Synthesis and self-assembly of DHDHAB were performed. A low critical ...
Source: Colloids and Surfaces B: Biointerfaces - July 28, 2018 Category: Biochemistry Source Type: research

Enzyme immobilization on protein-resistant PNIPAAm brushes: impact of biotin linker length on enzyme amount and catalytic activity
This report describes the functionalization of alkyne-PNIPAAm brushes with two biotin linkers of different poly(ethylene glycol) (PEG) spacer length via click chemistry and the subsequent immobilization of streptavidin-conjugated horseradish peroxidase (SA-HRP) by the strong interaction between biotin and streptavidin. Spectroscopic ellipsometry is used to quantify the biotin and the SA-HRP amount on the PNIPAAm brushes. Enzyme activities are determined by UV‑vis spectroscopy. A better accessibility to the alkyne-functionalized chain ends for the short biotin-PEG3 linker leads to a higher biotin amount on these PNIPAAm b...
Source: Colloids and Surfaces B: Biointerfaces - July 27, 2018 Category: Biochemistry Source Type: research

Molecular basis for RGD-containing peptides supporting adhesion and self-renewal of human pluripotent stem cells on synthetic surface
In this study, we focused on the Arg-Gly-Asp (RGD) sequence and integrin receptors, which constitute the major recognition system for cell adhesion. Several new RGD-containing peptides were designed by altering the amino acids surrounding the RGD sequence. We investigated the ability of these peptides to sustain hPSC survival, and identified the Ac-KGGPQVTRGDTYRAY sequence, which was capable of supporting cell reprogramming, long-term self-renewal and lineage differentiation. In addition, this report demonstrates that the introduction of mutations in the amino acids surrounding the RGD sequence is a good strategy to design...
Source: Colloids and Surfaces B: Biointerfaces - July 27, 2018 Category: Biochemistry Source Type: research

Mitoxantrone-loaded chitosan/hyaluronate polyelectrolyte nanoparticles decorated with amphiphilic PEG derivates for long-circulating effect
Publication date: Available online 26 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jiajia Wang, Sajid Asghar, Xin Jin, Zhipeng Chen, Lin Huang, Qineng Ping, Li Zong, Yanyu XiaoAbstractPoly (ethylene glycol) (PEG) and its derivatives are not only used to improve the stability of drug-loaded nanoparticles but also prolong their stay in blood for extended durations. We, hereby, report mitoxantrone loaded polyelectrolyte nanoparticles (MTO-PENPs) based on the hyaluronic acid (HA) and chitosan hydrochloride (HCS) complexed with amphiphilic PEG derivatives, carboxylated PEG (100) monostearate (PGMC, MTO-CPEN...
Source: Colloids and Surfaces B: Biointerfaces - July 27, 2018 Category: Biochemistry Source Type: research

Nanostructured electrochemical immunosensor for detection of serological alkaline phosphatase
In this study we present the development and analytical application of an impedimetric immunosensor based in gold nanoparticle-decorated CNT, which characteristics are desirable in implantable biosensors. The device effectively detects ALP within blood serum, a complex biological fluid where most expressed proteins can be found. Robustness and high sensitivity were attained by immobilizing covalently anti-ALP antibody as a specific probe towards ALP. Cyclic voltammetry, electrochemical impedance spectroscopy and atomic force microscopy were used to characterize the sensor system throughout mounting steps and real sample te...
Source: Colloids and Surfaces B: Biointerfaces - July 26, 2018 Category: Biochemistry Source Type: research

Study on the development of wax emulsion with liquid crystal structure and its moisturizing and frictional interactions with skin
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Ye Wang, Jiusheng Li, Yazhuo Shang, Xiangqiong ZengAbstractThe moisturizing and frictional interactions between emulsion and skin have a significant influence on the moisturizing efficacy and sensory properties of cosmetics. To deeply understand the interactions, we developed a kind of wax emulsion with liquid crystal structure and studied their interactions with dry, semi-moist and fully-moist skin. The moisturizing interaction was studied by using skin hydration test and tape stripping analysis, and it was found that wit...
Source: Colloids and Surfaces B: Biointerfaces - July 26, 2018 Category: Biochemistry Source Type: research

Preparation of self-assembled nanoparticles of ɛ-polylysine - sodium alginate: a sustained-release carrier for antigen delivery
Publication date: Available online 25 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Jing Yuan, Lu Guo, Sijia Wang, Dan Liu, Xia Qin, Lili Zheng, Chunlian Tian, Xiaohu Han, Ran Chen, Ronghuan YinAbstractLow immunogenicity prohibits the widespread use of subunit vaccine against infectious diseases and cancers. Hence, a new generation of adjuvants and delivery systems is indispensable for more potent antigen-specific immune responses. Predominantly, nanoparticles formulated from biodegradable polymers are being widely explored as carriers of novel vaccines owing to their outstanding natural properties. We ...
Source: Colloids and Surfaces B: Biointerfaces - July 26, 2018 Category: Biochemistry Source Type: research

Biological and Optical Properties of Sol–Gel Derived ZnO Using Different percentages of silver contents
Publication date: Available online 25 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zohra Nazir Kayani, Maryam Anwar, Zeb Saddiqe, Saira Riaz, Shahzad NaseemAbstractThe role of Ag dopant on ZnO thin films are studied. Ag doped ZnO thin films were deposited by sol gel dip coating on glass substrates. The X-ray diffraction analysis shows hexagonal wurtzite with preferred orientation along the (101) plane. The crystallite size decreases from 33.40 nm to 28.37 nm with increase in silver doping percentage. Optical examination shows that the band gap decrease with an increase in the Ag doping in...
Source: Colloids and Surfaces B: Biointerfaces - July 26, 2018 Category: Biochemistry Source Type: research

Exopolysaccharide from Bacillus cereus VK1: Enhancement, characterization and its potential application in heavy metal removal
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Ramaraju Kalpana, Maria Joseph Angelaalincy, Balaji Viswanath Kamatchirajan, Vairathevar Sivasamy Vasantha, Balasubramaniem Ashokkumar, Venkatachalam Ganesh, Perumal VaralakshmiAbstractIncreasing interests in industrialization invites acute or chronic toxicity into the environment resulting in serious ecological risks. This can be efficiently handled by bioremediation techniques. Hence in the present study, an exopolysaccharide (EPS) producing bacterium was isolated and identified as Bacillus cereus VK1 by 16S rRNA charact...
Source: Colloids and Surfaces B: Biointerfaces - July 26, 2018 Category: Biochemistry Source Type: research

Corrigendum to “An investigation of konjac glucomannan-keratin hydrogel scaffold loaded with Avena sativa extracts for diabetic wound healing” [Colloids Surf. B Biointerfaces 165 (2018) 92–102]
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Praveen Krishna Veerasubramanian, Ponrasu Thangavel, Ramya Kannan, Sudip Chakraborty, Balaji Ramachandran, Lonchin Suguna, Vignesh Muthuvijayan (Source: Colloids and Surfaces B: Biointerfaces)
Source: Colloids and Surfaces B: Biointerfaces - July 26, 2018 Category: Biochemistry Source Type: research

In-vitro evaluation of cytotoxic and antioxidant properties of drugs solubilized in EO-PO star block copolymer micelles
Publication date: Available online 24 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Urjita Sheth, Anita BahadurAbstractSelf-assembly of a biocompatible, nontoxic, commercially available star shaped polyethylene oxide-polypropylene oxide block copolymer, Tetronics®1107 (mol. wt. = 15000, % ethylene oxide = 70, and HLB 18-23) in aqueous solution was examined using surface tension, fluorescence, viscosity, cloud point and dynamic light scattering. The copolymer being very hydrophilic shows poor surface activity and forms thermo-, pH and salt responsive nanosize core-shell mi...
Source: Colloids and Surfaces B: Biointerfaces - July 25, 2018 Category: Biochemistry Source Type: research

Phase Behavior of DSPC/PEG40St Mixtures at Higher Emulsifier Contents
Publication date: Available online 24 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Sevgi Kilic, Elif Seniz BolukcuAbstractPhase behaviors of 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) and polyoxyethylene(40)stearate (PEG40St) were investigated with Langmuir monolayer isotherms and Brewster angle microscopy (BAM) imaging at DSPC/PEG40St molar ratios ranging from 9:1 to 5:5. Two plateaus were found in the Langmuir isotherms which were relatively shorter for the 9:1 mixture and extended significantly by increasing the PEG40St content, indicating that the PEG40St squeezed out whereas more emulsifier...
Source: Colloids and Surfaces B: Biointerfaces - July 24, 2018 Category: Biochemistry Source Type: research

Antimicrobial and biocompatible fluorescent hydroxyapatite-chitosan nanocomposite films for biomedical applications
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Somtirtha Banerjee, Biswajoy Bagchi, Suman Bhandary, Arpan Kool, Nur Amin Hoque, Prosenjit Biswas, Kunal Pal, Pradip Thakur, Kaustuv Das, Parimal Karmakar, Sukhen DasAbstractDevelopment of fluorescent erbium doped hydroxyapatite (eHAp)-chitosan nanocomposite film is reported. Nanocrystalline eHAp has been synthesized by hydrothermal assisted precipitation method using erbium (III) ions as dopant. Physico-chemical characterization by UV/Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (...
Source: Colloids and Surfaces B: Biointerfaces - July 24, 2018 Category: Biochemistry Source Type: research

Coaxial extrusion bioprinted shell-core hydrogel microfibers mimic glioma microenvironment and enhance the drug resistance of cancer cells
In this study, shell-glioma stem cell GSC23/core-glioma cell line U118 (G/U) hydrogel microfibers with high cell viability were constructed by coaxial extrusion bioprinting. It was found that core-U118 cells gradually proliferated to form fiber-like cell aggregates and the interactions between cell-cell and cell-extracellular matrix (ECM) were increased. Furthermore, compared with shell/core-U118 (U) hydrogel microfibers, the expressions of matrix metalloproteinase-2 (MMP2), MMP9, vascular endothelial growth factor receptor-2 (VEGFR2) and O6-methylguanine-DNA methyltransferase (MGMT) which are related to tumor invasion and...
Source: Colloids and Surfaces B: Biointerfaces - July 24, 2018 Category: Biochemistry Source Type: research

Effects of aligned and random fibers with different diameter on cell behaviors
Publication date: Available online 21 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Xuyan Li, Xiaofeng Wang, Dongshuang Yao, Jing Jiang, Xin Guo, Yanhong Gao, Qian Li, Changyu ShenAbstractTissue-engineered vascular grafts show promise as a treatment for patients with diseased or damaged arteries. The formation of a confluent endothelium lumen on scaffold is one of the key issues in vascular tissue engineering. The endothelial layer is composed of endothelial cells (ECs) aligned along the long axis of the vessel. The contact guidance effect is believed to fundamentally impact cell morphology, alignment, ...
Source: Colloids and Surfaces B: Biointerfaces - July 21, 2018 Category: Biochemistry Source Type: research

Interaction of green silver nanoparticles with model membranes: Possible role in the antibacterial activity
Publication date: Available online 21 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Anike P.V. Ferreyra Maillard, Pablo R. Dalmasso, Beatriz A. López de Mishima, Axel HollmannAbstractSilver nanoparticles (AgNPs) constitute a very promising approach for overcoming the emergence of antibiotic resistance bacteria. Although their mode of action could be related with membrane damage, the AgNPs-lipid membrane interaction is still unclear. In this sense, the present work investigated the interaction of model lipid membranes with AgNPs coated with different capping agents such as citrate (C-AgNPs) and ph...
Source: Colloids and Surfaces B: Biointerfaces - July 21, 2018 Category: Biochemistry Source Type: research

The effect of surface charge on the cytotoxicity and uptake of carbon quantum dots in human umbilical cord derived mesenchymal stem cells
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Junyan Yan, Shulan Hou, Yanzhen Yu, Yong Qiao, Tongqian Xiao, Yan Mei, Zhijun Zhang, Bin Wang, Chih-Ching Huang, Chia-Hua Lin, Guangli SuoAbstractCarbon quantum dots (CQDs) are emerging as an ideal agent for efficient stem cell labeling. In current study, we synthesized a series of CQDs carrying different surface charges by changing the mass ratio of diammonium citrate (DC) and spermidine (Spd), and evaluated the effects of different surface charges on the cytotoxicity, cellular uptake, stability in human umbilical cord de...
Source: Colloids and Surfaces B: Biointerfaces - July 21, 2018 Category: Biochemistry Source Type: research

Graphene oxide nanosheets increase Candida albicans killing by pro-inflammatory and reparative peritoneal macrophages
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Rosalía Diez-Orejas, María José Feito, Mónica Cicuéndez, Laura Casarrubios, José María Rojo, María Teresa PortolésAbstractGraphene oxide (GO) is a new nanomaterial with different potential biomedical applications due to its excellent physicochemical properties and ease of surface functionalization. Macrophages play key roles in the control of fungal infections preventing invasive candidiasis by both limiting the growth of the opportunistic fungal pathogen Candi...
Source: Colloids and Surfaces B: Biointerfaces - July 21, 2018 Category: Biochemistry Source Type: research

Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption
In this study, we present a comparison of the antioxidant activity of catalase immobilized on gold nanoparticles (AuNPs) by two methods: i) directly on the surface of AuNPs (non-specific immobilization), and ii) via chemical bonding using a linker (specific immobilization). Quantification of the enzyme amount adsorbed on the nanoparticle surface was determined by native-polyacrylamide gel electrophoresis (native-PAGE). Colloidal stability of AuNPs before and after the enzyme immobilization was monitored with dynamic light scattering (DLS) and UV-Vis spectroscopy. The size of the metallic core was determined by scanning-tra...
Source: Colloids and Surfaces B: Biointerfaces - July 21, 2018 Category: Biochemistry Source Type: research

Synthesis and characterization of fluorinated anatase nanoparticles and subsequent N-doping for efficient visible light activated photocatalysis
Publication date: Available online 20 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): I. Milošević, S. Rtimi, A. Jayaprakash, B. van Driel, B. Greenwood, A. Aimable, M. Senna, P. BowenAbstractFluorinated-titanium dioxide (TiO2-F) nanoparticles in a pure anatase polymorph was precipitated from solution by hydrolysis of titanium oxychloride, using urea and ammonia as precipitation agents and potassium fluoride as a source of fluorine anion. A further wet attrition milling in presence of glycine completed by a heat treatment allowed an additional nitrogen doping of TiO2 (TiO2-F&N-HT). The morphol...
Source: Colloids and Surfaces B: Biointerfaces - July 21, 2018 Category: Biochemistry Source Type: research

BSA-Based Cu2Se Nanoparticles with Multistimuli-Responsive Drug Vehicles for Synergistic Chemo-Photothermal Therapy
Publication date: Available online 19 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zhou Liu, Leung Chan, Xiaoting Ye, Yan Bai, Tianfeng ChenAbstractFunctionalized-nanoparticles have been developed as novel therapeutic delivery platform for simultaneous drug loading and therapy over the past decade. Rationally-designed biocompatible nanosystem simultaneously with multistimuli-responsive property and synergistic therapeutic potential are highly desirable for modern biological applications. Herein, Cu2Se nanoparticles (Cu2SeNPs) with suitable size have been functionalized by bull serum albumin (BSA) throu...
Source: Colloids and Surfaces B: Biointerfaces - July 20, 2018 Category: Biochemistry Source Type: research

Adhesion and Stiffness of Biotin-Superavidin Bonds
Publication date: Available online 19 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Bahman Farzi, Jan Scrimgeour, Cetin CetinkayaAbstractA non-invasive vibrational spectroscopy technique is introduced and utilized to characterize the average spring constant of a single Superavidin (SAv)-Biotin (Bi).polyethylene glycol (PEG) ligand receptor complex as well as the effective Young’s modulus and adhesion of a layer formed by the SAv-Bi.PEG ligand-receptors. In the reported experiments, SAv coated Polystyrene (PS) particles are deposited on a layer of Bi.PEG receptors, bound to a silicon (Si) substrate...
Source: Colloids and Surfaces B: Biointerfaces - July 20, 2018 Category: Biochemistry Source Type: research

Detailed characterization of POSS-poly(ethylene glycol) interaction with model phospholipid membrane at the air/water interface
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Marta Skrzypiec, Krystyna ProchaskaAbstractPolyhedral oligomeric silsesquioxanes (POSS) derivatives have been receiving remarkable attention due to their potential biomedical application and therefore understanding molecular mechanism of their interaction with cell membranes should be studied at molecular level. Here we investigate the binary mixture of an open silsesquioxane cage POSS-poly(ethylene glycol) (POSS-PEG) and 1,2-myristoyl-sn-glycero-3-phosphoethanolamine (DMPE) as a representative of phospholipid located in b...
Source: Colloids and Surfaces B: Biointerfaces - July 19, 2018 Category: Biochemistry Source Type: research

Fluorescent activatable gadofullerene nanoprobes as NIR-MR dual-modal in vivo imaging contrast agent
Publication date: 1 November 2018Source: Colloids and Surfaces B: Biointerfaces, Volume 171Author(s): Cheng Li, Huan Huang, Rongli Cui, Juan Li, Xihong Guo, Huanli Yao, Bing Liu, Binggang Xu, Ying Li, Shuaichao Liu, Jinquan Dong, Gengmei Xing, Baoyun SunAbstractDual mode imaging technology is widely developed to achieve the early-stage precision cancer diagnosis. Here we designed a dual-modal magnetic resonance/near infrared fluorescence optical imaging contrast agent (GdF-SS-NIR783) with the fluorescence activatable and safer gadofullerene. The nanoprobes were fabricated by conjugating the gadofullerene derivatives with a...
Source: Colloids and Surfaces B: Biointerfaces - July 19, 2018 Category: Biochemistry Source Type: research

Reduced inflammatory response by incorporating magnesium into porous TiO2 coating on titanium substrate
Publication date: Available online 17 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Xuezhong Li, Qianli Huang, Ling Liu, Weihong Zhu, Tarek A. Elkhooly, Yong Liu, Qingling Feng, Qingxiang Li, Shaoqiang Zhou, Yin Liu, Hong WuAbstractThe implant materials with proper anti-inflammatory and osteogenic properties may be promising for orthopedic applications. The inflammatory response induced by biomaterials has been regarded as one of the critical factors in determining in vivo fate of implants. Therefore, a novel bone biomaterial should have inflammation regulatory effects instead of being completely bio-in...
Source: Colloids and Surfaces B: Biointerfaces - July 18, 2018 Category: Biochemistry Source Type: research

The elucidation of surrounding alginate gels on the pollutants degradation by entrapped nanoscale zero-valent iron
Publication date: Available online 17 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Kexin Yi, Zhixuan Fan, Jinping Tang, Anwei Chen, Jihai Shao, Liang Peng, Qingru Zeng, Si LuoAbstractThe adsorption capacity of calcium alginate (Ca-alginate) beads was evaluated by measuring the removal of three organic compounds with different charges (malachite green, p-chlophenol and methyl orange). The diffusion was investigated in Ca-alginate hydrogel as a function of solute charge. It was found that an increased electrostatic attraction between the hydrogel and solute would improve the mobility of solute and hence ...
Source: Colloids and Surfaces B: Biointerfaces - July 18, 2018 Category: Biochemistry Source Type: research

Functionalization of Graphene Family Nanomaterials for Application in Cancer Therapy
Publication date: Available online 17 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Duarte de Melo-Diogo, Rita Lima-Sousa, Cátia G. Alves, Elisabete C. Costa, Ricardo O. Louro, Ilídio J. CorreiaAbstractGraphene family nanomaterials’ (GFN) ability to interact with near-infrared light has propelled their application in cancer photothermal therapy. Furthermore, the graphitic lattice of GFN can adsorb different types of molecules, which has motivated their use in cancer drug delivery. However, the direct application of GFN in cancer therapy is severely hindered by their poor colloidal st...
Source: Colloids and Surfaces B: Biointerfaces - July 18, 2018 Category: Biochemistry Source Type: research