Targeting malignant melanoma with physical plasmas
Publication date: June 2018Source: Clinical Plasma Medicine, Volume 10Author(s): Gabriella Pasqual-Melo, Rajesh Kumar Gandhirajan, Ingo Stoffels, Sander BekeschusAbstractMelanoma is the deadliest form of cutaneous neoplasia. With a five-year survival rate of only 5–19%, metastatic melanoma presents severe challenges in clinical therapies. In addition, palliation is often problematic due to large numbers of fast growing metastasis. This calls for new therapeutic avenues targeting highly aggressive melanoma in palliative patients. One recently suggested innovative approach for eradication of topical tumor lesions is the ap...
Source: Clinical Plasma Medicine - July 5, 2018 Category: Research Source Type: research
Connecting basic cold plasma technology to dermato-oncology
Publication date: June 2018Source: Clinical Plasma Medicine, Volume 10Author(s): Carsten Weishaupt, Steffen EmmertAbstractCold atmospheric plasma was intensively investigated for medical applications in the last decade and a new field of plasma medicine emerged. Basic components of cold plasma and their biomedical effects were deciphered and mainly relate to redox-biologic effects. With regard to wound treatment, cold plasma already reached standard medical care status. In terms of plasma effects in oncology, only preliminary research data are available. This emerging and highly dynamic field of plasma medicine in oncology...
Source: Clinical Plasma Medicine - July 5, 2018 Category: Research Source Type: research
Plasma-treated medium tunes the inflammatory profile in murine bone marrow-derived macrophages
Publication date: Available online 30 June 2018Source: Clinical Plasma MedicineAuthor(s): Sander Bekeschus, Lukas Scherwietes, Eric Freund, Kim Rouven Liedtke, Christine Hackbarth, Thomas von Woedtke, Lars-Ivo ParteckeAbstractMacrophages are essential drivers of tumor rejection as well as tumor promotion. Especially tumor-associated macrophages (TAM) phenotypically resemble tumor-supporting alternatively activated macrophages (M2). Targeting their phenotype has long been a matter of preclinical research in oncology. Cold physical plasma and plasma-treated medium has recently been recognized as a new possible interventional...
Source: Clinical Plasma Medicine - July 5, 2018 Category: Research Source Type: research
Plasma-treated medium tunes the inflammatory profile in murine bone marrow-derived macrophages
Publication date: Available online 30 June 2018 Source:Clinical Plasma Medicine Author(s): Sander Bekeschus, Lukas Scherwietes, Eric Freund, Kim Rouven Liedtke, Christine Hackbarth, Thomas von Woedtke, Lars-Ivo Partecke Macrophages are essential drivers of tumor rejection as well as tumor promotion. Especially tumor-associated macrophages (TAM) phenotypically resemble tumor-supporting alternatively activated macrophages (M2). Targeting their phenotype has long been a matter of preclinical research in oncology. Cold physical plasma and plasma-treated medium has recently been recognized as a new possible interventio...
Source: Clinical Plasma Medicine - June 30, 2018 Category: Research Source Type: research
Side effects in cold plasma treatment of advanced oral cancer —Clinical data and biological interpretation
Conclusion Understanding the lack of severe side effects in plasma medicine, the role of radical oxygen species (ROS) and radical nitrogen species (RNS) is discussed, proposing a model in which CAP is not a direct effector of antitumor action but rather triggers a singlet oxygen-mediated switch-on effect on the specific target, leading to reactivation of intercellular ROS/RNS-dependent apoptosis signaling in tumor cells. As these processes are strictly restricted to the specific targets and as normal tissue is devoid of the required target, it is neither harmed nor affected. (Source: Clinical Plasma Medicine)
Source: Clinical Plasma Medicine - May 11, 2018 Category: Research Source Type: research
Side effects in cold plasma treatment of advanced oral cancer – Clinical data and biological interpretation
Conclusion Understanding the lack of severe side effects in plasma medicine, the role of radical oxygen species (ROS) and radical nitrogen species (RNS) is discussed, proposing a model in which CAP is not a direct effector of antitumor action but rather triggers a singlet oxygen-mediated switch-on effect on the specific target, leading to reactivation of intercellular ROS/RNS-dependent apoptosis signaling in tumor cells. As these processes are strictly restricted to the specific targets and as normal tissue is devoid of the required target, it is neither harmed nor affected. (Source: Clinical Plasma Medicine)
Source: Clinical Plasma Medicine - April 27, 2018 Category: Research Source Type: research
Effect Of Plasma Pen Treatment And Plasma Activated Medium (PAM) On Cancer And Normal Cells
Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Dominika Sersenová, Helena Gbelcová, Adam Polakovič, Vanda Repiská, Zdenko Machala Non-thermal atmospheric pressure plasma has recently found an ever growing use in medicine; including development of new cancer treatments. The most significant factor, produced by plasma that influence cancer cells are reactive oxygen and nitrogen species (RONS). RONS react with the surrounding air, cellular aqueous media and with cells themselves; however, the exact mechanism of their interaction with the cells is not yet fully under...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Sensitivity Of Murine Melanoma Cells B16 To Plasma Needle Treatment
Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Jorge Humberto Serment Guerrero, Karina Giron Romero, Régulo López-Callejas, Rosendo Peña-Eguiluz Melanoma is the most dangerous form of skin cancer originated from the pigment-producing melanocytes in the basal layer of the epidermis. Its capability to produce metastasis, along with the development of quimio or radioresistance makes this kind of cancer very dangerous and hard to heal, so is important an early detection as well as the search of new treatments. B16 murine cell line has been used extensively as a model f...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Technical advance: High Content Imaging of 2D and 3D tumor spheroids co-cultured with THP-1 monocytes following Plasma Treatment
In this study, we aimed to determine the Toxicity Potency (TC50) of human melanoma derived spheroids (SK-Mel28) following cold plasma treatment (kINPen) under following conditions: a) plasma-treated tumor spheroid monoculture, and b) plasma-treated tumor spheroid/THP1 co-culture. Tumor spheroids toxicity was enumerated by dead cell staining and normalized to nuclear Hoechst stain following time-lapse microscopy in a high content confocal microscope (Operatta™, Perkin Elmer). Results indicate that cold plasma monotherapy significantly induces a dose-dependent toxicity in spheroids. This toxicity was enhanced upon co-cultu...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Effect Of Low-Temperature Plasma On Metastatic Processes Of Solid Tumors In Vitro
Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Angela Privat-Maldonado, Evelien Smits, Annemie Bogaerts Cancer metastasis is one of the leading causes of mortality in patients with solid tumors [1]. The complex metastatic process involves cell migration and remodeling of the tumor microenvironment, among other factors. The intrinsic limitations of conventional cancer therapies for solid tumors have motivated the development and application of alternative technologies. In this context, the use of low-temperature plasmas (LTPs) has been explored in vitro and in vivo with...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Plasma as adjuvant in melanoma treatment via mitochondrial targeting
Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Gabriella Pasqual-Melo, Rajesh Gandhirajan, Thomas von Woedtke, Sander Bekeschus Cutaneous melanoma is a highly aggressive malignancy and has rapidly increased over the past several decades [1]. Although the clinical biology and pathogenesis of melanoma are well understood, the prognosis remains poor with limited therapeutic options in the metastatic stage of the disease [2]. Reactive oxygen species (ROS) may be drivers of carcinogenesis and may cause oxidative damage to several cellular components, so it is generally con...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Microarry Analysis Identifies Activation Of Multiple Signalling Pathways In Primary Prostate Epithelial Cells By Low Temperature Plasma
Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): John Packer, Adam Hirst, Fiona Frame, Norman Maitland, Deborah O’Connell We have previously reported the killing effects of Low Temperature Plasma (LTP) on primary prostate epithelial cells, of both normal and cancerous origin, via necrosis [1] following initial DNA damage. However it was evident that a population of cells was resistant to LTP treatment. To discern possible survival mechanisms in treated cells; the gene expression of 6 samples (4 cancers and 2 matched normal) was analysed 2 hours post-treatment, on a t...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Influence of Cold Atmospheric Plasma on Cytokine Expression in Human Osteosarcoma Cells
Conclusion CAP treatment led to an attenuation of OS cell proliferation through the induction of apoptosis. Moreover, CAP influences cytokines expression in OS cells which further influences target cell proliferation, immunomodulation and chemotaxis. In addition, cytokines impact tumor angiogenesis and metastasis development. Our results confirm a modulating influence of CAP on cell-cell interactions and systemic processes. This makes CAP a promising therapy option, especially for chemoresistant entities. (Source: Clinical Plasma Medicine)
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Mitochondrial Complex IV Inhibition and Exogenous Oxidants Potently Synergize in Melanoma Cell Death Induction
In this study, we investigated murine (metastatic B16F10 and non-metastatic B16F0) as well as human (SK-Mel 28) melanoma cells, and compared them with murine (primary fibroblasts) and human (HaCaT keratinocytes) non-cancer cells. Mitochondrial complex IV inhibition was realized using sodium azide (NaN3) and potassium cyanide (KCN) and pharmacological inhibitor ADDA5. Exogenous oxidants were generated using cell culture medium that has been treated with the cold physical plasma effluent of an atmospheric pressure argon plasma jet (kINPen). This plasma-treated medium was subsequently applied to different cells. Metabolic act...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
Immunogenic Cell Death In Murine Colon-Carcinoma Cells Following Exposure To Cold Physical Plasma-Treated Saline Solution
Publication date: February 2018 Source:Clinical Plasma Medicine, Volume 9, Supplement Author(s): Eric Freund, Christine Hackbarth, Lars-Ivo Partecke, Sander Bekeschus Diffuse peritoneal metastasis of gastrointestinal tumors is a life-threatening complication in end-stage tumor patients. Standard of care is hyperthermic intraperitoneal chemotherapy (HIPEC) and/or radiation therapy, both associated with significant side effects [1]. Previous studies have suggested that cold physical plasma may present a new anti-tumor tool with few to none side effects [2]. Moreover, plasma-induced reactive species can be transferred t...
Source: Clinical Plasma Medicine - April 10, 2018 Category: Research Source Type: research
