Soy isoflavone genistein attenuates the efficacy of immune checkpoint therapy in C57BL/6 mice inoculated with B16F1 melanoma and a high PD-L1 expression level reflects tumor resistance
In this study, we examined the effect of genistein on immune checkpoint blockade therapy against B16F1 melanoma tumors. Mice treated with genistein or anti-programmed death (PD)-1 antibody showed a significant decrease in tumor growth. However, treatment with genistein had no effect on or attenuated the efficacy of immune checkpoint therapy. The percentages of T cell receptor (TCR)β+CD4+ and TCRβ+CD8+ cells and the concentrations of interferon-γ and tumor necrosis factor-α in tumor tissue were not different among the experimental groups. A significant difference was also not found in microbe composition. Interestingly,...
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: Mako Yamamoto Yuko Tanaka Risako Takeda Akiko Nakamoto Mariko Nakamoto Hideo Yagita Tohru Sakai Source Type: research
Erratum
J Clin Biochem Nutr. 2024 Jan;74(1):90. doi: 10.3164/jcbn.22-105_Erratum. Epub 2023 Nov 3.ABSTRACT[This corrects the article DOI: 10.3164/jcbn.22-105.].PMID:38292120 | PMC:PMC10822761 | DOI:10.3164/jcbn.22-105_Erratum (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Source Type: research
Single oral administration of quercetin glycosides prevented acute hyperglycemia by promoting GLUT4 translocation in skeletal muscles through the activation of AMPK in mice
In conclusion, single oral administration of quercetin glycosides prevented a blood sugar spike by promoting glucose transporter 4 translocation through activating the CAMKKβ/AMPK signaling pathway.PMID:38292121 | PMC:PMC10822753 | DOI:10.3164/jcbn.23-30 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: Yoko Yamashita Hao Jiang Fukiko Okada Tomoya Kitakaze Yasukiyo Yoshioka Hitoshi Ashida Source Type: research
The role of fat indices as factors leading to sarcopenia in older adults residing in underpopulated areas
J Clin Biochem Nutr. 2024 Jan;74(1):70-73. doi: 10.3164/jcbn.23-33. Epub 2023 Aug 1.ABSTRACTSimplifying the diagnostic criteria for sarcopenia is key to establishing effective interventions. Herein, we aimed to clarify novel diagnostic factors. We calculated novel fat indices [total fat index (TFI) and limb fat index (LFI)] and clarified factors leading to pre-sarcopenia and sarcopenia in 594 enrolled older adults. Physical measurements [height, weight, body mass index (BMI), gait speed, grip strength, and skeletal muscle mass] were performed. Sarcopenia was determined using established diagnostic criteria (pre-sarcopenia,...
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: Yasutaka Mizukami Hidenori Onishi Yuta Mifuku Masafumi Kubota Ryouko Ikeda Hiroyuki Hayashi Osamu Yamamura Source Type: research
Background factors of idiopathic peptic ulcers and optimal treatment methods: a multicenter retrospective Japanese study
This study investigated the trends in idiopathic peptic ulcers, examined the characteristics of refractory idiopathic peptic ulcer, and identified the optimal treatment. The characteristics of 309 patients with idiopathic peptic ulcer were examined. We allocated idiopathic peptic ulcers that did not heal after 8 weeks' treatment (6 weeks for duodenal ulcers) to the refractory group and those that healed within this period to the healed group. The typical risk factors for idiopathic peptic ulcer (atherosclerosis-related underlying disease or liver cirrhosis complications) were absent in 46.6% of patients. Absence of gastric...
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: Noriyuki Nakajima Toshihisa Takeuchi Ryota Hokari Kazuyuki Narimatsu Katsunori Iijima Shigeto Koizumi Kunio Kasugai Masahide Ebi Akihito Nagahara Tsutomu Takeda Toshihiko Tomita Shinichiro Shinzaki Kazuhiro Mizukami Kazunari Murakami Nobuaki Yagi Rieko Mu Source Type: research
Effect of fat ingestion on postprandial oxidative status in healthy young women: a pilot study
This study offers an insight into the acute effects of fat ingestion on leukocyte activity and provides a methodology for monitoring postprandial oxidative status.PMID:38292124 | PMC:PMC10822754 | DOI:10.3164/jcbn.23-50 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: Kozo Takeuchi Kimiko Kazumura Kaori Kuzawa Yukiko Hatano Masashi Nagai Michitaka Naito Source Type: research
Dietary antioxidants and flavonoids intake, and their association with inflammation and oxidative stress parameters in asthmatic women: a case-control study
This study aimed to compare the dietary intake of antioxidants in asthmatic and healthy women, evaluating their potential associations with inflammation and oxidative stress. This study analyzed 30 asthmatic and 30 healthy women's lung function, anthropometry, biochemical parameters, and dietary antioxidant intake using a 161-itemized semi-quantitative food frequency questionnaire. Additionally, the study explored connections between serum inflammatory markers and oxidative stress indicators in relation to dietary intake of antioxidant nutrients and flavonoids. Asthmatic women exhibited higher serum IL-6 levels and lower t...
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: G ülen Suna Olgun Keskin İkbal Özen Küçükçetin Aylin Ayaz Source Type: research
Enhancement of cytotoxic effects with ALA-PDT on treatment of radioresistant cancer cells
In this study, we attempted to clear this mechanism using X-ray resistant cancer cells. X-ray resistant cells produce high amounts of mitochondria-derived ROS, which enhanced nuclear translocation of NF-κB, resulting in increased NO production. Moreover, the expression of PEPT1 that imports 5-aminolevulinic acid, the precursor of photosensitizers, was upregulated in X-ray resistant cancer cells. This was accompanied by an increase in intracellular 5-aminolevulinic acid-derived porphyrin accumulation, resulting in enhancement of PDT-induced cytotoxicity. Therefore, effective accumulation of photosensitizers induced by ROS ...
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: Takafumi Ikeda Hiromi Kurokawa Hiromu Ito Kiichiro Tsuchiya Hirofumi Matsui Source Type: research
Vitamin D insufficiency and disease risk in the elderly
J Clin Biochem Nutr. 2024 Jan;74(1):9-16. doi: 10.3164/jcbn.23-59. Epub 2023 Aug 26.ABSTRACTVitamin D insufficiency, milder than deficiency, is common, and a risk of various diseases. Since vitamin D exert diverse actions, both skeletal and non-skeletal, its insufficiency is a risk of various diseases including osteoporosis, sarcopenia, cardiovascular disease, cancer, and even mortality. Regarding the association of vitamin D status and disease risk, a marked discrepancy exists between the results from the observational studies and intervention studies, mostly yielding the positive and negative results in the former and la...
Source: Clinical Biochemistry - January 31, 2024 Category: Biochemistry Authors: Kiyoshi Tanaka Misora Ao Junko Tamaru Akiko Kuwabara Source Type: research
Preparing for another Ebola Outbreak: The impact of viral inactivation methods on commonly measured biochemistry analytes in plasma and urine
CONCLUSION: Reliable results for commonly measured electrolytes, enzymes and proteins can be obtained after viral inactivation by Triton X-100 treatment at room temperature. However, heat inactivation has significant negative impact on routine biochemistry enzymes and alternative testing processes should be explored.PMID:38242342 | DOI:10.1016/j.clinbiochem.2024.110718 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - January 19, 2024 Category: Biochemistry Authors: Janet Zhou Franceska Sevilleno Fari Rokhforooz Jennifer Taher Source Type: research
Homeodomain Transcription Factors Nkx2.2 and Pax6 as Novel Biomarkers for Meningioma Tumor Treatment
Indian J Clin Biochem. 2024 Jan;39(1):47-59. doi: 10.1007/s12291-022-01085-1. Epub 2022 Aug 30.ABSTRACTMeningioma is a common brain tumour which has neither a specific detection nor treatment method. The Sonic hedgehog (Shh) cell signaling pathway is a crucial regulatory pathway of mammalian organogenesis and tumorigenesis including meningioma. Shh cell signalling pathway cascade function by main transcription factor Gli1 and which further regulates in its downstream to Pax6 and Nkx2.2. This current study is aimed to explore the regulation of the Sonic hedgehog-Gli1 cell signaling pathway and its potential downstream targe...
Source: Clinical Biochemistry - January 15, 2024 Category: Biochemistry Authors: Shirin Farheen Mubeena Mariyath Pm Suhailur Rehman Md Fakhrul Hoda Yakhlesh Gupta Asif Ali Kunzang Chosdol Mehdi H Shahi Source Type: research
Heparin Binding Protein in Early Differential Diagnosis of Bacterial Meningitis
Indian J Clin Biochem. 2024 Jan;39(1):118-123. doi: 10.1007/s12291-022-01066-4. Epub 2022 Aug 9.ABSTRACTHeparin-binding protein is a serine protease that is mobilized rapidly from emigrating polymorphonuclear leukocytes that acts as a chemoattractant activator of monocyte and macrophages. We investigated the potential role and efficacy of serum and cerebrospinal fluid heparin binding protein in differentiating bacterial meningitis from tuberculosis and viral meningitis. A case diagnosed with acute bacterial meningitis (n:37), viral meningitis (n:30) and tuberculous meningitis (n:30) was included in this study. The diagnosi...
Source: Clinical Biochemistry - January 15, 2024 Category: Biochemistry Authors: Emine Siber Namiduru Mustafa Namiduru İlkay Karaoğlan Enes Erba ğci Source Type: research
Role of Supply Chain Management in Clinical Laboratory
Indian J Clin Biochem. 2024 Jan;39(1):1-2. doi: 10.1007/s12291-023-01178-5. Epub 2023 Dec 28.NO ABSTRACTPMID:38223002 | PMC:PMC10784235 | DOI:10.1007/s12291-023-01178-5 (Source: Clinical Biochemistry)
Source: Clinical Biochemistry - January 15, 2024 Category: Biochemistry Authors: Prasenjit Mitra Shruti Gupta Praveen Sharma Source Type: research
Evaluation of Bone Turnover Markers Such as Osteoprotegerin, Sclerostin and Dickkopf-1 in Subclinical Hyperthyroidism
In this study, it was aimed to assess effects of subclinical hyperthyroidism (SH) on bone metabolism using osteoprotegerin (OPG), sclerostin, Dickkopf-1 (DKK1) and biochemical parameters. This cross-sectional prospective study included 40 patients with SH and 40 euthyroid controls. Serum OPG, sclerostin, DKK-1, type-1 procollagen, C-terminal polypeptide (CTx) and 24-hours urine N-terminal telopeptide (NTx) were measures using ELISA kit. Bone mineral density measurements were performed using dual energy X-ray absorptiometry (DEXA). Risk for 10-years hip and major fracture was estimated by Turkish version of FRAX. No signifi...
Source: Clinical Biochemistry - January 15, 2024 Category: Biochemistry Authors: Ay şe Elverdi Özbek H üseyin Korkmaz Mehmet S özen S üleyman Hilmi İpekçi Sedat Abu şoğlu Cem Onur K ıraç Ali Ünlü Levent Kebap çılar Source Type: research
The 'Insertion/Deletion' Polymorphism, rs4340 and Diabetes Risk: A Pilot Study from a Hospital Cohort
Indian J Clin Biochem. 2024 Jan;39(1):124-129. doi: 10.1007/s12291-022-01076-2. Epub 2022 Jul 26.ABSTRACTThe insertion/deletion, I/D polymorphism, in the gene encoding Angiotensin Converting Enzyme, ACE is a popular genetic marker for cardiovascular disease, CVD. With alarming rise in diabetes, the risk of CVD among Indian subjects is further enhanced. The present study explored the role of ACE I/D polymorphism, rs4340 as a genetic marker and its association with diabetes. Genomic DNA, isolated from a cohort of 410 urban subjects attending our hospital, was genotyped using polymerase chain reaction followed by electrophore...
Source: Clinical Biochemistry - January 15, 2024 Category: Biochemistry Authors: Manali Shah Anjali Gupta Mitali Talekar Krishna Chaaithanya Priyanka Doctor Sandra Fernandes Rahul Doctor A Rosalind Marita Source Type: research
