Bupleurum polysaccharides ameliorated renal injury in diabetic mice associated with suppression of HMGB1-TLR4 signaling
This study suggested that the protection provided by BPs might affect through the interruption of HMGB1-TLR4 pathway, leading to the inhibition of renal inflammation and fibrotic process. (Source: Chinese Journal of Natural Medicines)
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection
In this study, we found that GGD had moderate anti-IAV activity in vitro. GGD was more effective when given before the viral infection and targeted the viral attachment and replication stages rather than the internalization stage. In vivo, GGD treatment reduced thevirus titers of lung tissue significantly and improved the survival rate, lung index, and pulmonary histopathological changes in H1N1-infected mice. We observed the changes in several key immuno-related indexes in GGD administrated H1N1-infected mice with anti-IAV drug oseltamivir phosphate as the control. GGD treatment decreased the expression of TNF-α and impr...
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

Pyridocarbazole alkaloids from Ochrosia borbonica: lipid-lowering agents inhibit the cell proliferation and adipogenesis of 3T3-L1 adipocyte via intercalating into supercoiled DNA
Publication date: September 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 9Author(s): Yao-Hao XU, Wei LI, Yong RAO, Zhi-Shu HUANG, Sheng YINAbstractBioassay-guided fractionation of an ethanolic extract of Ochrosia borbonica led to the isolation of two known pyridocarbazole alkaloids, ellipticine (1) and 9-methoxyellipticine (2), and six known monoterpenoid indole alkaloids (3–8). Lipid-lowering assay in 3T3-L1 cell model revealed that 1 and 2 could significantly inhibit the lipid droplet formation (EC50 = 0.41 and 0.92 μmol·L−1, respectively) and lower triglyceride levels by 50%–60% at the conc...
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

Potential hepatic and renal toxicity induced by the biflavonoids from Ginkgo biloba
Publication date: September 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 9Author(s): Yun-Ying LI, Xiao-Yan LU, Jia-Li SUN, Qing-Qing WANG, Yao-Dan ZHANG, Jian-Bing ZHANG, Xiao-Hui FANAbstractEvidence continues to grow on potential health risks associated with Ginkgo biloba and its constituents. While biflavonoid is a subclass of the flavonoid family in Ginkgo biloba with a plenty of pharmacological properties, the potential toxicological effects of biflavonoids remains largely unknown. Thus, the aim of this study was to investigate the in vitro and in vivo toxicological effects of the biflavonoids fro...
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

The inhibition mechanism of the uptake of lamivudine via human organic anion transporter 1 by Stellera chamaejasme L. extracts
Publication date: September 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 9Author(s): Lan-Ying PAN, Kui ZENG, Li LI, Yan LOU, Su ZENGAbstractStellera chamaejasme L. is a traditional Chinese medicine with a long history to treat stubborn skin ulcer, and it also has antiviral and antitumor effects. Neochamaejasmine B (NCB), Neochamaejasmine A (NCA) and Chamaechromone (CMC) are the major components in dried roots of Stellera chamaejasme L.. Our studies suggested that NCB, NCA and CMC are inhibitors of Organic anion transporter 1 (OAT1). OAT1 is encoded by solute carrier family 22 member 6 gene (SLC22A6) i...
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

Role of kaempferol to increase bioavailability and pharmacokinetics of nifedipine in rats
Publication date: September 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 9Author(s): Ji-Won PARK, Jin-Seok CHOIAbstractHerein, the purpose of this study is to evaluate the effects of kaempferol on bioavailability and pharmacokinetics of nifedipine and its metabolite dehydronifedipine in rats. The experimental design is based on with or without kaempferol in the oral and intravenous administration of nifedipine in rats. Moreover, the pharmacokinetic parameters including nifedipine and dehydronifedipine were evaluated in rats.The in vitro studies ofkaempferol were investigated on P-glycoprotein (P-gp) a...
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

New aporphine alkaloids with selective cytotoxicity against glioma stem cells from Thalictrum foetidum
Publication date: September 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 9Author(s): Cai-Feng DING, Zhi DAI, Hao-Fei YU, Xu-Dong ZHAO, Xiao-Dong LUOAbstractSeven new isoquinoline alkaloids, 9-(2′-formyl-5′, 6′-dimethoxyphenoxy)-1, 2, 3, 10-tetramethoxy dehydroaporphine (1), 9-(2′-formyl-5′, 6′-dimethoxyphenoxy)-1, 2, 3, 10-tetramethoxy oxoaporphine (2), 3-methoxy-2′-formyl oxohernandalin (3), (−)-9-(2′-methoxycarbonyl-5′, 6′-dimethoxyphenoxy)-1, 2, 3, 10-tetramethoxy aporphine (4), (−)-2′-methoxycarbonyl thaliadin (5), (−)-9-(2′-methoxyethyl-5′, 6′-dimethoxyphenoxy)-1...
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

3, 4-seco-Labdane diterpenoids from the leaves of Callicarpa nudiflora with anti-inflammatory effects
Publication date: September 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 9Author(s): Hong-Gang WANG, Fu-Kang LUO, Xiang LEI, Yun-Da YAO, Guo-Chao LIAO, Zhong-Qiu LIU, Zhi-Hong JIANG, Hua ZHOU, Peng WUAbstractFour new 3, 4-seco-labdane diterpenoids, nudiflopenes J-M, were isolated from the leaves of Callicarpa nudiflora along with six known compounds. The structures of these diterpenoids were determined by comprehensive spectroscopic analysis. All the isolated compounds were evaluated for their inhibitory effects on NO production in LPS-stimulated RPMs and RAW264.7 cells. The results suggest that nudif...
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

Traditional Chinese medicine extraction method by ethanol delivers drug-like molecules
In this study, we selected 6 common TCMs that are rich in chemistry to investigate whether the TCM extraction methods deliver molecules with drug-like physical chemical properties. Six TCM herbal materials were extracted by water, 95% ethanol, and sequential hexane, dichloromethane and methanol. The extracts were analyzed by HPLC and 1H NMR. Isolation on one of the extracts yielded 32 compounds, their physical chemical properties were analyzed by Instant JChem. Our results showed that ethanol extraction, which mimics TCM wine tincture, delivered compounds with physical chemical properties compliant to Lipinski's rule of 5....
Source: Chinese Journal of Natural Medicines - September 14, 2019 Category: Complementary Medicine Source Type: research

Prediction of the globally ecological suitability of Panax quinquefolius by the geographic information system for global medicinal plants (GMPGIS)
In this study, the areas with potential ecological suitability for P. quinquefolius were predicted using the geographic information system for global medicinal plants (GMPGIS) based on 476 occurrence points and 19 bioclimatic variables. The results indicate that the new ecologically suitable areas for P. quinquefolius are East Asia and the mid-eastern Europe, which are mainly distributed in China, Russia, Japan, Ukraine, Belarus, North Korean, South Korea, andRomania. Under global climate change scenarios, the suitable planting areas for P. quinquefolius would be increased by 9.16%–30.97%, and expandingnorth and west ove...
Source: Chinese Journal of Natural Medicines - September 11, 2019 Category: Complementary Medicine Source Type: research

An in vitro study on interaction of anisodine and monocrotaline with organic cation transporters of the SLC22 and SLC47 families
Publication date: July 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 7Author(s): Jia-Yin CHEN, Jürgen Brockmöller, Mladen V. Tzvetkov, Li-Jun WANG, Xi-Jing CHENABSTRACTCurrent study systematically investigated the interaction of two alkaloids, anisodine and monocrotaline, with organic cation transporter OCT1, 2, 3, MATE1 and MATE2-K by using in vitro stably transfected HEK293 cells. Both anisodine and monocrotaline inhibited the OCTs and MATE transporters. The lowest IC50 was 12.9 µmol·L−1 of anisodine on OCT1 and the highest was 1.8 mmol·L−1 of monocrotaline on OCT2. Anisodine was a substrate...
Source: Chinese Journal of Natural Medicines - September 11, 2019 Category: Complementary Medicine Source Type: research

XingNaoJing injections protect against cerebral ischemia/reperfusion injury and alleviate blood-brain barrier disruption in rats, through an underlying mechanism of NLRP3 inflammasomes suppression
Publication date: July 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 7Author(s): Xiao-Yu QU, Yue-Ming ZHANG, Li-Na TAO, Huan GAO, Jing-Hui ZHAI, Jing-Meng SUN, Yan-Qing SONG, Si-Xi ZHANGABSTRACTThe aim of this study was to explore the neuroprotective effect and mechanism of XingNaoJing injections (XNJ) on cerebral ischemia injury and blood-brain barrier (BBB) disruption. Middle cerebral artery occlusion (MCAO) method was applicated to establish the model of cerebral ischemia/reperfusion (I/R) injury in rats. BBB permeability after I/R injury was assessed with the leaking amount of Evans Blue and the ex...
Source: Chinese Journal of Natural Medicines - September 11, 2019 Category: Complementary Medicine Source Type: research

Catharmus tinctorius volatile oil promote the migration of mesenchymal stem cells via ROCK2/Myosin light chain signaling
This study indicated that CTVO promotes MSC migration through enhancing ROCK2 mRNA and protein expressions. MSC migration induced by CTVO was blunted by ROCK2 inhibitor, which also decreased myosin light chain (MLC) phosphorylation. Meanwhile, the siRNA for ROCK2 inhibited the effect of CTVO on MSC migration ability and attenuated MLC phosphorylation, suggesting that CTVO may promote BMSC migration via the ROCK2/MLC signaling. Taken together, this study indicates that C. tinctorius volatile oil could enhance MSC migration via ROCK2/MLC signaling in vitro. C. tinctorius volatile oil-targeted therapy could be a beneficial st...
Source: Chinese Journal of Natural Medicines - September 11, 2019 Category: Complementary Medicine Source Type: research

A hepatoprotection study of Radix Bupleuri on acetaminophen-induced liver injury based on CYP450 inhibition
Publication date: July 2019Source: Chinese Journal of Natural Medicines, Volume 17, Issue 7Author(s): Yu-Xin WANG, Yi DU, Xia-Fei LIU, Fang-Xiu YANG, Xiao WU, Li TAN, Yi-Hong LU, Jing-Wei ZHANG, Fang ZHOU, Guang-Ji WANGABSTRACTWe investigated the potential hepatoprotective effect of Radix Bupleuri (RB) by inducing acute liver injury (ALI) in an animal model using acetaminophen (APAP) after pretreatment with RB aqueous extract for three consecutive days. Compared to those of the APAP group, the biochemical and histological results of the RB pretreatment group showed lower serumaspartate transaminase (AST) and alanine transa...
Source: Chinese Journal of Natural Medicines - September 11, 2019 Category: Complementary Medicine Source Type: research

Chinese medicine Yu-Ping-Feng-San attenuates allergic inflammation by regulating epithelial derived pro-allergic cytokines
This study aimed to investigate the mechanisms of Yu-Ping-Feng-San (YPFS) on attenuating allergic inflammation in the initial stage of atopic dermatitis (AD). AD mouse model was established with fluorescein isothiocyanate (FITC) sensitization and elicitation. Epithelial barrier structure was observed with transmission electron microscope. The populations of dendritic cells (DCs) and group 2 innate lymphoid cells (ILC2s) were detected by flow cytometry. Human immortalized keratinocyte (HaCaT) cells were stimulated with Poly(I:C)/TNF-α in vitro to assessthymic stromal lymphopoietin (TSLP), interleukin (IL)-33 and nuclear fa...
Source: Chinese Journal of Natural Medicines - September 11, 2019 Category: Complementary Medicine Source Type: research