miR-101-2, miR-125b-2 and miR-451a act as potential tumor suppressors in gastric cancer through regulation of the PI3K/AKT/mTOR pathway
Conclusions Our expression analyses and in vitro functional assays suggest that miR-101-2, miR-125b-2 and miR-451a act as potential tumor suppressors in primary GCs as well as in GC-derived AGS cells. (Source: Cellular Oncology)
Source: Cellular Oncology - October 12, 2015 Category: Cancer & Oncology Source Type: research

Inhibition of Ras-mediated signaling pathways in CML stem cells
Conclusions A closer investigation of the influence of Ras-mediated signaling pathways on CML progression to blast crisis is warranted to uncover new directions for targeted therapies, particularly in cases that are resistant to current tyrosine kinase inhibitors. (Source: Cellular Oncology)
Source: Cellular Oncology - October 12, 2015 Category: Cancer & Oncology Source Type: research

Methylation biomarkers for pleomorphic lobular breast cancer - a short report
Conclusion This is the first report to identify TP73, RASSF1A, MLH1 and BRCA1 as possible biomarkers to distinguish pleomorphic ILC from classic ILC and IDC. (Source: Cellular Oncology)
Source: Cellular Oncology - September 21, 2015 Category: Cancer & Oncology Source Type: research

Decreased CDK10 expression correlates with lymph node metastasis and predicts poor outcome in breast cancer patients - a short report
Abstract Background Cyclin-dependent kinase 10 (CDK10) has recently been identified as a tumor suppressor and, concordantly, its encoding gene has frequently been found to be inactivated in various human cancers. Here, we examined the expression status of CDK10 in a panel of primary human breast cancers and evaluated its correlation with clinicopathological parameters and clinical outcome. Methods Western blotting was used to assess CDK10 protein levels in 20 paired breast cancer tissues and adjacent noncancerous tissues. In addition, immunohistochemistr...
Source: Cellular Oncology - September 21, 2015 Category: Cancer & Oncology Source Type: research

Identification of miR-10b, miR-26a, miR-146a and miR-153 as potential triple-negative breast cancer biomarkers
Conclusion Our work provides evidence for the involvement of specific miRNAs in triple-negative breast cancer development through regulating BRCA1 expression. (Source: Cellular Oncology)
Source: Cellular Oncology - September 21, 2015 Category: Cancer & Oncology Source Type: research

Consensus reference gene(s) for gene expression studies in human cancers: end of the tunnel visible?
Conclusions Based on the analysis of the data at hand, we firstly recommend that in each study the suitability of candidate reference gene(s) should carefully be evaluated in order to yield reliable differential gene expression data. Secondly, we recommend that a combination of PPIA and either GAPDH, ACTB, HPRT and TBP, or appropriate combinations of two or three of these genes, should be employed in future studies, to ensure that results from different studies on different human cancers can be harmonized. This approach will ultimately increase the depth of our understanding of gene expression signatures across h...
Source: Cellular Oncology - September 18, 2015 Category: Cancer & Oncology Source Type: research

Role of JNK and NF-κB in mediating the effect of combretastatin A-4 and brimamin on endothelial and carcinoma cells
Conclusions Our data indicate that cellular JNK and Rho kinase activities are crucial for the cytotoxic and cytoskeleton reorganizing effects of brimamin on endothelial cells. In addition, we found that in resistant carcinoma cells and xenografts brimamin can induce down-regulation of anti-apoptotic NF-κB expression and signaling. Its chemical properties and efficacy against clinically relevant cancer entities make brimamin a promising candidate vascular-disrupting agent. (Source: Cellular Oncology)
Source: Cellular Oncology - September 10, 2015 Category: Cancer & Oncology Source Type: research

DNA methylation patterns in EBV-positive and EBV-negative Hodgkin lymphomas
Conclusions Our results indicate that epigenetic changes frequently occur in both EBV-positive and EBV-negative HL. The rates of these changes were found to vary according to clinico-pathological parameters. These observations probably reflect the multitude of factors involved in HL development and the complexity of their interactions with genetic and/or hormonal factors. (Source: Cellular Oncology)
Source: Cellular Oncology - September 8, 2015 Category: Cancer & Oncology Source Type: research

The carboxy-terminal domain of connexin 43 (CT-Cx43) modulates the expression of p53 by altering miR-125b expression in low- grade human breast cancers
Conclusions Our data suggest a mechanism by which CT-Cx43 may regulate cell proliferation. Targeting of CT-Cx43 and/or miR-125b may be instrumental for therapeutic intervention in human breast cancer. (Source: Cellular Oncology)
Source: Cellular Oncology - September 3, 2015 Category: Cancer & Oncology Source Type: research

NF-κB signaling in cancer stem cells: a promising therapeutic target?
Conclusion Although still preliminary, recent evidence shows that such targeted strategies may be useful in adjuvant chemo-preventive settings. (Source: Cellular Oncology)
Source: Cellular Oncology - August 29, 2015 Category: Cancer & Oncology Source Type: research

MYCN concurrence with SAHA-induced cell death in human neuroblastoma cells
Conclusions Our results suggest that SAHA may be used as a single-drug treatment option for neuroblastomas with an amplified MYCN gene, and as an adjuvant treatment option for all neuroblastomas. (Source: Cellular Oncology)
Source: Cellular Oncology - August 26, 2015 Category: Cancer & Oncology Source Type: research

Prognostic value of cytokeratin-7 mRNA expression in peripheral whole blood of advanced lung adenocarcinoma patients
Conclusions Our data show that KRT7 mRNA expression measured by RT-qPCR serves as a sensitive approach for the molecular detection of KRT7-positive CTC-resembling A549 cells in peripheral whole blood. The KRT7 mRNA levels measured were not significantly associated with the response to chemotherapy or the survival of patients with advanced lung AC. Additional studies are required to establish the possible clinical significance of KRT7 mRNA expression in whole blood after chemotherapy. (Source: Cellular Oncology)
Source: Cellular Oncology - August 26, 2015 Category: Cancer & Oncology Source Type: research

Manipulation of tumor metabolism for therapeutic approaches: ovarian cancer-derived cell lines as a model system
Conclusion A combination of metabolic inhibitors and reactive oxygen species-generating therapies, such as irradiation, may effectively enhance the therapeutic response in particularly metabolically highly active (ovarian) tumors. (Source: Cellular Oncology)
Source: Cellular Oncology - August 19, 2015 Category: Cancer & Oncology Source Type: research

Metformin and epithelial ovarian cancer therapeutics
Conclusions Our data indicate that metformin can induce apoptosis in both primary ovarian cancer cells and in SKOV-3 cells. When metformin was combined with carboplatin or paclitaxel, an increased apoptotic activity was observed, implicating a chemo-adjuvant potential. (Source: Cellular Oncology)
Source: Cellular Oncology - August 12, 2015 Category: Cancer & Oncology Source Type: research

Expression of the microRNA regulators Drosha, Dicer and Ago2 in non-small cell lung carcinomas
Conclusions Our results point at a role of Drosha, Dicer and Ago2 in the development of NSCLC and suggest that Dicer may be implicated in the progression of these tumors to advanced stages. (Source: Cellular Oncology)
Source: Cellular Oncology - July 31, 2015 Category: Cancer & Oncology Source Type: research

Anti-tumor effect of emodin on gynecological cancer cells
Conclusions Our results suggest that emodin exhibits an anti-tumor effect on gynecological cancer-derived cells, possibly through multiple mechanisms including the induction of apoptosis and autophagy, the arrest of the cell cycle, and the inhibition of angiogenesis. Our findings may provide a basis for the design of potential emodin-based strategies for the treatment of gynecological tumors. (Source: Cellular Oncology)
Source: Cellular Oncology - July 11, 2015 Category: Cancer & Oncology Source Type: research

RRM1 expression is associated with the outcome of gemcitabine-based treatment of non-small cell lung cancer patients–a short report
Conclusions The meta-analysis reported here indicates that RRM1 expression is associated with the response rate and overall survival rate of advanced NSCLC patients treated with gemcitabine-based chemotherapy. Additional phase III randomized trials are required to confirm our current findings. (Source: Cellular Oncology)
Source: Cellular Oncology - June 20, 2015 Category: Cancer & Oncology Source Type: research

The bone marrow metastasis niche in retinoblastoma
Conclusions The various BM metastasis-related molecular changes identified to date may be instrumental for a better diagnosis, prognosis and classification of Rb patients, as well as for the development of novel comprehensive (targeted) therapies. (Source: Cellular Oncology)
Source: Cellular Oncology - June 11, 2015 Category: Cancer & Oncology Source Type: research

Myoepithelial and luminal breast cancer cells exhibit different responses to all-trans retinoic acid
Conclusions Our results show that the luminal epithelial (LEP) and myoephitelial (MEP) mammary LM38-P subpopulations respond differently to ATRA, i.e., the LEP subpopulation responds with increased cell cycle arrest and apoptosis and the MEP subpopulation responds with increased senescence and adhesion, thereby decreasing its invasive capacity. Finally, we identified a third subpopulation with stem/progenitor cell characteristics within the LM38-LP mammary adenocarcinoma cell line, which appears to be non-responsive to ATRA. (Source: Cellular Oncology)
Source: Cellular Oncology - June 5, 2015 Category: Cancer & Oncology Source Type: research

Human renal carcinoma cells respond to Newcastle disease virus infection through activation of the p38 MAPK/NF-κB/IκBα pathway
Conclusion Our data provide evidence for involvement of the p38 MAPK/NF-κB/IκBα pathway in NDV infection and subsequent induction of apoptosis in ccRCC cells. (Source: Cellular Oncology)
Source: Cellular Oncology - May 1, 2015 Category: Cancer & Oncology Source Type: research

HAUSP regulates c-MYC expression via de-ubiquitination of TRRAP
Conclusions From our results we conclude that HAUSP may act as an oncogenic protein that can modulate c-MYC expression via TRRAP. Our results provide a new context in which HAUSP may play a role in cancer cell signalling. (Source: Cellular Oncology)
Source: Cellular Oncology - April 30, 2015 Category: Cancer & Oncology Source Type: research

Chromosome 17 copy number changes in male breast cancer
Conclusions In MBC chromosome 17 shows less complex rearrangements and fewer copy number changes compared to FBC. Frequent gains of 17q, encompassing two distinct amplicons, and losses of 17p were observed, but no whole chromosome 17 polyploidies. Only NEUROD2 gains seem to have an independent prognostic impact. These results suggest different roles of chromosome 17 aberrations in male versus female breast carcinogenesis. (Source: Cellular Oncology)
Source: Cellular Oncology - April 24, 2015 Category: Cancer & Oncology Source Type: research

KLF15 in breast cancer: a novel tumor suppressor?
Conclusions Our results indicate that nuclear KLF15 expression suppresses breast cancer cell proliferation at least partially through p21 up-regulation and subsequent cell cycle arrest. This is a first study addressing the role of KLF15 in breast cancer development. (Source: Cellular Oncology)
Source: Cellular Oncology - April 13, 2015 Category: Cancer & Oncology Source Type: research

Anti-proliferative, apoptotic and signal transduction effects of hesperidin in non-small cell lung cancer cells
Conclusions Our results indicate that hesperidin elicits an in vitro growth inhibitory effect on NSCLC cells by modulating immune response-related pathways that affect apoptosis. When confirmed in vivo, hesperidin may serve as a novel anti-proliferative agent for non-small cell lung cancer. (Source: Cellular Oncology)
Source: Cellular Oncology - April 10, 2015 Category: Cancer & Oncology Source Type: research

Associations between polymorphisms in the SYK promoter and susceptibility to sporadic colorectal cancer in a Southern Han Chinese population - a short report
Conclusions The SYK −803 A> T genotypes TA and TT are independent risk factors for CRC development in Han Chinese in Southern China, and an association with TA + TT genotypes appears predominant among younger patients, male patients, patients with a high BMI, and patients who smoke or drink alcohol. (Source: Cellular Oncology)
Source: Cellular Oncology - April 1, 2015 Category: Cancer & Oncology Source Type: research

PI3K/AKT pathway-mediated regulation of p27 Kip1 is associated with cell cycle arrest and apoptosis in cervical cancer
Conclusion Our data suggest that p27Kip1 down-regulation in cervical cancer cells is primarily regulated through PI3K/AKT-mediated proteasomal degradation. The observed synergistic effect of the MG132 and LY294002 inhibitors may form a basis for the design of novel cervical cancer therapies. (Source: Cellular Oncology)
Source: Cellular Oncology - March 28, 2015 Category: Cancer & Oncology Source Type: research

Aberrant SOX11 promoter methylation is associated with poor prognosis in gastric cancer
Conclusions Our results indicate that aberrant SOX11 gene promoter methylation may underlie its down-regulation in GC. SOX11 gene promoter hyper-methylation may serve as a biomarker to predict the clinical outcome of GC. (Source: Cellular Oncology)
Source: Cellular Oncology - March 24, 2015 Category: Cancer & Oncology Source Type: research

Personalized cancer medicine: next steps in the genomic era
(Source: Cellular Oncology)
Source: Cellular Oncology - February 27, 2015 Category: Cancer & Oncology Source Type: research

ECRG4 acts as a tumor suppressor and as a determinant of chemotherapy resistance in human nasopharyngeal carcinoma
Conclusion Our data suggest that methylation-mediated suppression of the ECRG4 gene occurs frequently in NPC and that restoration of its expression may have therapeutic benefits. (Source: Cellular Oncology)
Source: Cellular Oncology - February 24, 2015 Category: Cancer & Oncology Source Type: research

Cross-resistance to clinically used tyrosine kinase inhibitors sunitinib, sorafenib and pazopanib
Conclusions Our data indicate that tumor cells can develop (cross-) resistance to TKIs, and that such resistance includes increased intracellular drug accumulation accompanied by increased lysosomal storage. Transient (cross-) resistance was found to occur for several of the TKIs tested, but not for everolimus, indicating that switching from a TKI to a mTOR inhibitor may be an attractive therapeutic option. (Source: Cellular Oncology)
Source: Cellular Oncology - February 11, 2015 Category: Cancer & Oncology Source Type: research

MicroRNA-138-5p regulates pancreatic cancer cell growth through targeting FOXC1
Abstract Purpose The prognosis of pancreatic cancer ranks among the worst of all cancer types, which is primarily due to the fact that during the past decades little progress has been made in its diagnosis and treatment. Here, we set out to investigate the role of microRNA 138 (miR-138-5p) in the regulation of pancreatic cancer cell growth and to assess its role as putative therapeutic target. Methods qRT-PCR was used to examine the expression of miR-138-5p in 8 pancreatic cancer cell lines and 18 primary human pancreatic cancer samples. A lentivirual ve...
Source: Cellular Oncology - February 10, 2015 Category: Cancer & Oncology Source Type: research

Intrinsic cancer subtypes-next steps into personalized medicine
Abstract Recent technological advances have significantly improved our understanding of tumor biology by means of high-throughput mutation and transcriptome analyses. The application of genomics has revealed the mutational landscape and the specific deregulated pathways in different tumor types. At a transcriptional level, multiple gene expression signatures have been developed to identify biologically distinct subgroups of tumors. By supervised analysis, several prognostic signatures have been generated, some of them being commercially available. However, an unsupervised approach is required to discover a pr...
Source: Cellular Oncology - January 14, 2015 Category: Cancer & Oncology Source Type: research

Germline oncopharmacogenetics, a promising field in cancer therapy
Abstract Pharmacogenetics (PGx) is the study of the relationship between inter-individual genetic variation and drug responses. Germline variants of genes involved in drug metabolism, drug transport, and drug targets can affect individual response to medications. Cancer therapies are characterized by an intrinsically high toxicity; therefore, the application of pharmacogenetics to cancer patients is a particularly promising method for avoiding the use of inefficacious drugs and preventing the associated adverse effects. However, despite continuing efforts in this field, very few labels include information about ge...
Source: Cellular Oncology - January 9, 2015 Category: Cancer & Oncology Source Type: research

Anti-tumor activity of the TGF-β receptor kinase inhibitor galunisertib (LY2157299 monohydrate) in patient-derived tumor xenografts
Conclusions From our data we conclude that intrinsic, tumor-dependent TGF-β signaling does not fully explain the anti-tumor effect of galunisertib. Hence, in vivo xenograft models may be more appropriate than in vitro clonogenic assays to assess the anti-tumor activity of TGF-β inhibitors such as galunisertib. (Source: Cellular Oncology)
Source: Cellular Oncology - January 9, 2015 Category: Cancer & Oncology Source Type: research

Molecular basis of chronic lymphocytic leukemia diagnosis and prognosis
Conclusions Specific chromosomal abnormalities and gene mutations may serve as diagnostic and prognostic indicators for disease progression and survival. The identification of these anomalies by state-of-the-art molecular (cyto)genetic techniques such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), single nucleotide polymorphism (SNP) microarray-based genomic profiling and next-generation sequencing (NGS) can be of paramount help for the clinical management of these patients, including optimal treatment design. The efficacy of novel therapeutics should to be tested according...
Source: Cellular Oncology - January 7, 2015 Category: Cancer & Oncology Source Type: research

Mitochondrial induction as a potential radio-sensitizer in lung cancer cells - a short report
Conclusion Mitochondrial induction by DCA may serve as a radio-sensitizer in non-small cell lung cancer. (Source: Cellular Oncology)
Source: Cellular Oncology - January 7, 2015 Category: Cancer & Oncology Source Type: research

Chlorogenic acid inhibits hypoxia-induced angiogenesis via down-regulation of the HIF-1α/AKT pathway
Conclusions Our data indicate that CGA plays a role in the suppression of angiogenesis via inhibition of the HIF-1α/AKT pathway. CGA may represent a novel therapeutic option for the treatment of (lung) cancer. (Source: Cellular Oncology)
Source: Cellular Oncology - January 6, 2015 Category: Cancer & Oncology Source Type: research

Synergy of leptin/STAT3 with HER2 receptor induces tamoxifen resistance in breast cancer cells through regulation of apoptosis-related genes
Conclusions Our study provides novel evidence indicating that synergy between the leptin/Ob-Rb/STAT3 signalling pathway and the HER2 receptor protects tamoxifen-treated HER2 over-expressing cells from the inhibitory effect of tamoxifen through differential regulation of apoptosis-related genes. (Source: Cellular Oncology)
Source: Cellular Oncology - December 25, 2014 Category: Cancer & Oncology Source Type: research

Epidermal growth factor receptor mutations in East European non-small cell lung cancer patients
In this study 1427 NSCLC samples were included. DNA was extracted from either formalin-fixed paraffin embedded (FFPE) tissues or cytology specimens and analyzed for the presence of 29 recurrent EGFR gene mutations using SARMS PCR. Results EGFR gene mutations were found to occur significantly more often in female than in male patients (19.4 % vs. 5.4 %; p 
Source: Cellular Oncology - December 23, 2014 Category: Cancer & Oncology Source Type: research

Somatic mutations of amino acid metabolism-related genes in gastric and colorectal cancers and their regional heterogeneity - a short report
Conclusions Our data indicate that genes known to be involved in amino acid metabolism recurrently acquire somatic mutations in MSH-H GCs and MSH-H CRCs and that, in addition, mutation ITH does occur in at least some of these tumors. Together, these data suggest that metabolic reprogramming may play a role in the etiology of MSI-H GCs and CRCs. Our data also suggest that ultra-regional mutation analysis is required for a more comprehensive evaluation of the mutation status in these tumors. (Source: Cellular Oncology)
Source: Cellular Oncology - December 2, 2014 Category: Cancer & Oncology Source Type: research

Apoptosis induction by an analog of curcumin (BDMC-A) in human laryngeal carcinoma cells through intrinsic and extrinsic pathways
Conclusions From our results we conclude that BDMC-A can induce apoptosis in Hep-2 laryngeal carcinoma cells more effectively than curcumin, and that this activity can be attributed to the presence of a hydroxyl group at the ortho position within this compound. (Source: Cellular Oncology)
Source: Cellular Oncology - December 1, 2014 Category: Cancer & Oncology Source Type: research

Relationship between therapeutic efficacy of doxorubicin-transferrin conjugate and expression of P-glycoprotein in chronic erythromyeloblastoid leukemia cells sensitive and resistant to doxorubicin
Conclusion Our data suggest that the newly developed DOX-TRF conjugate is a less P-gp dependent substrate than free DOX and, consequently, may be used in a clinical setting to increase treatment efficacy in resistant human tumors. (Source: Cellular Oncology)
Source: Cellular Oncology - November 20, 2014 Category: Cancer & Oncology Source Type: research

2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD) inhibits human ovarian cancer cell proliferation
Conclusion Our data indicate that TCDD can suppress human ovarian cancer cell proliferation via the AhR signaling pathway and that TCDD exhibits an anti-proliferative activity in at least a subset of human ovarian cancer cells. (Source: Cellular Oncology)
Source: Cellular Oncology - November 18, 2014 Category: Cancer & Oncology Source Type: research

E4BP4 is a repressor of epigenetically regulated SOSTDC1 expression in breast cancer cells
Conclusion From our results we conclude that the transcriptional repressor E4BP4 plays a role in repressing epigenetically regulated SOSTDC1 expression in breast cancer cells, which can be reverted by E4BP4 silencing. (Source: Cellular Oncology)
Source: Cellular Oncology - October 22, 2014 Category: Cancer & Oncology Source Type: research

Deoxyhypusine synthase (DHPS) inhibitor GC7 induces p21/Rb-mediated inhibition of tumor cell growth and DHPS expression correlates with poor prognosis in neuroblastoma patients
Abstract Purpose Neuroblastoma (NB) is an aggressive pediatric malignancy that typically occurs in infants and children under the age of 5 years. High-stage tumors relapse frequently even after aggressive multimodal treatment, resulting in therapy resistance and eventually in patient death. Clearly, new biologically-targeted drugs are needed that more efficiently suppress tumor growth and prevent relapse. We and others previously showed that polyamines such as spermidine play an essential role in NB tumorigenesis and that DFMO, an inhibitor of the central polyamine synthesis gene ODC, is ...
Source: Cellular Oncology - October 15, 2014 Category: Cancer & Oncology Source Type: research

Erratum to: Long non-coding RNAs in cancer: implications for personalized therapy
(Source: Cellular Oncology)
Source: Cellular Oncology - October 6, 2014 Category: Cancer & Oncology Source Type: research

Molecular imaging of targeted therapies with positron emission tomography: the visualization of personalized cancer care
Conclusion Molecular imaging with radiolabelled targeted anticancer drugs has great potential for the improvement of personalized cancer care. The non-invasive quantification of drug accumulation in tumours and normal tissues provides understanding of the biodistribution in relation to therapeutic and toxic effects. (Source: Cellular Oncology)
Source: Cellular Oncology - September 24, 2014 Category: Cancer & Oncology Source Type: research

Short hairpin RNA-mediated down-regulation of CENP-A attenuates the aggressive phenotype of lung adenocarcinoma cells
Conclusion Based on our results we conclude that down-regulation of CENP-A may attenuate the aggressive phenotype of lung adenocarcinoma cells. As such, CENP-A may serve as a promising therapeutic target for lung adenocarcinoma. (Source: Cellular Oncology)
Source: Cellular Oncology - September 17, 2014 Category: Cancer & Oncology Source Type: research

Gefitinib induces cytoplasmic translocation of the CDK inhibitor p27 and its binding to a cleaved intermediate of caspase 8 in non-small cell lung cancer cells
Conclusion Based on our results, we conclude that gefitinib-induced apoptosis is mediated by the interaction of p27 and caspase 8 in NSCLC cells carrying an activating EGFR mutation. (Source: Cellular Oncology)
Source: Cellular Oncology - September 17, 2014 Category: Cancer & Oncology Source Type: research

BMP9 regulates cross-talk between breast cancer cells and bone marrow-derived mesenchymal stem cells
Conclusions From our data we conclude that BMP9 inhibits the migration and invasion of breast cancer cells, and promotes the osteoblastic differentiation and proliferation of bone marrow-derived mesenchymal stem cells by regulating cross-talk between these two types of cells through the RANK/RANKL signaling axis. (Source: Cellular Oncology)
Source: Cellular Oncology - September 11, 2014 Category: Cancer & Oncology Source Type: research