DNA copy number alterations, gene expression changes and disease-free survival in patients with colorectal cancer: a 10 year follow-up
AbstractBackgroundDNA copy number alterations (CNAs) and gene expression changes have amply been encountered in colorectal cancers (CRCs), but the extent at which CNAs affect gene expression, as well as their relevance for tumor development, are still poorly defined. Here we aimed at assessing the clinical relevance of these parameters in a 10  year follow-up study.MethodsTumors and normal adjacent colon mucosa, obtained at primary surgery from 21 CRC patients, were subjected to (i) high-resolution array CGH (a-CGH) for the detection of CNAs and (ii) microarray-based transcriptome profiling for the detection of gene e...
Source: Cellular Oncology - October 5, 2016 Category: Cancer & Oncology Source Type: research

Identification of genetic variation in the lncRNA HOTAIR associated with HPV16-related cervical cancer pathogenesis
ConclusionsOur data indicate that rs2366152C not only has the potential to serve as a marker for singling out CaCx cases lacking metastatic molecular signatures, but also to explain the functional inactivation of HOTAIR in these cases, including the mechanism of its down-regulation. (Source: Cellular Oncology)
Source: Cellular Oncology - September 28, 2016 Category: Cancer & Oncology Source Type: research

Novel strategies for targeting leukemia stem cells: sounding the death knell for blood cancer
ConclusionsA growing body of evidence indicates that the selective targeting of LSCs represents a promising approach to improve disease outcome. Beyond doubt, the CSC hypothesis has added a new dimension to the area of anticancer research, thereby paving the way for shaping a new trend in cancer therapy. (Source: Cellular Oncology)
Source: Cellular Oncology - September 27, 2016 Category: Cancer & Oncology Source Type: research

Are disseminated tumor cells in bone marrow and tumor-stroma ratio clinically applicable for patients undergoing surgical resection of primary colorectal cancer? The Leiden MRD study
ConclusionsOur data indicate that the presence of DTCs in the BM of CRC patients is not associated with disease outcome. The TSR was, however, found to be associated with a worse overall survival, which indicates that for CRC the tumor microenvironment plays an important role in its behavior and prognosis. (Source: Cellular Oncology)
Source: Cellular Oncology - September 9, 2016 Category: Cancer & Oncology Source Type: research

Estrogen receptor expression and gene promoter methylation in non-small cell lung cancer - a short report
ConclusionsTaken together, our results indicate that decreased ER expression mediated by DNA methylation may play a role in NSCLC development. (Source: Cellular Oncology)
Source: Cellular Oncology - August 29, 2016 Category: Cancer & Oncology Source Type: research

NF κB activation demarcates a subset of hepatocellular carcinoma patients for targeted therapy
ConclusionFrom our data we conclude that NF κB signaling is activated in precancerous cirrhosis tissues and in a subset of HCCs. We found that ornithogalum exhibits NFκB targeting and stress relieving activities. NFκB inhibitors, including the active component of ornithogalum, may serve as putative preventive and targeted therapeutic agent s for at least a subset of HCCs in which the NFκB pathway is activated. These latter notions require further investigation in a translational context. (Source: Cellular Oncology)
Source: Cellular Oncology - August 25, 2016 Category: Cancer & Oncology Source Type: research

WT1, MSH6 , GATA5 and PAX5 as epigenetic oral squamous cell carcinoma biomarkers - a short report
ConclusionsOur data highlight the importance of epigenetically assessing OSCCs to identify key genes that may serve as diagnostic and prognostic biomarkers and, potentially, as candidate therapeutic targets. (Source: Cellular Oncology)
Source: Cellular Oncology - August 4, 2016 Category: Cancer & Oncology Source Type: research

Inactivation of the LKB1-AMPK signaling pathway does not contribute to salivary gland tumor development - a short report
ConclusionIn contrast to many other types of cancer, inactivation or downregulation of the LKB1/AMPK pathway does not substantially contribute to the pathogenesis of salivary gland tumors. (Source: Cellular Oncology)
Source: Cellular Oncology - August 1, 2016 Category: Cancer & Oncology Source Type: research

A network including PU.1, Vav1 and miR-142-3p sustains ATRA-induced differentiation of acute promyelocytic leukemia cells - a short report
ConclusionsOur results suggest the existence of a Vav1/PU.1/miR-142-3p network that supports ATRA-induced differentiation in APL-derived cells. Since selective regulation of miRNAs may play a role in the future treatment of hematopoietic malignancies, our results may provide a basis for the development of new therapeutic strategies to restore the expression of miR-142-3p. (Source: Cellular Oncology)
Source: Cellular Oncology - August 1, 2016 Category: Cancer & Oncology Source Type: research

The functional status of DNA repair pathways determines the sensitization effect to cisplatin in non-small cell lung cancer cells
Conclusions Our results indicate that the functional status of DNA repair pathways determine the sensitivity of NSCLC cells to cisplatin. Direct targeting of the pathway that is involved in cisplatin resistance may be an effective strategy to surmount cisplatin resistance in NSCLC. (Source: Cellular Oncology)
Source: Cellular Oncology - July 29, 2016 Category: Cancer & Oncology Source Type: research

Ovarian cancer-derived ascitic fluids induce a senescence-dependent pro-cancerogenic phenotype in normal peritoneal mesothelial cells
Conclusion Our results indicate that malignant ascitic fluids may contribute to ovarian cancer progression by accelerating the senescence of HPMCs. (Source: Cellular Oncology)
Source: Cellular Oncology - July 21, 2016 Category: Cancer & Oncology Source Type: research

Hypoxia-inducible microRNA-488 regulates apoptosis by targeting Bim in osteosarcoma
Conclusions Our data suggest that miRNA-488 may serve as a predictor of response to chemotherapy and as a therapeutic target in human osteosarcomas. (Source: Cellular Oncology)
Source: Cellular Oncology - July 4, 2016 Category: Cancer & Oncology Source Type: research

Nitric oxide increases the migratory activity of non-small cell lung cancer cells via AKT-mediated integrin αv and β1 upregulation
Conclusion Our data suggest a novel role of NO in the regulation of integrin expression and, concomitantly, the migratory capacity of NSCLC cells. (Source: Cellular Oncology)
Source: Cellular Oncology - July 4, 2016 Category: Cancer & Oncology Source Type: research

MGMT DNA repair gene promoter/enhancer haplotypes alter transcription factor binding and gene expression
Conclusions The data generated support our hypothesis that promoter haplotypes alter the binding of TFs to the MGMT P/E and, subsequently, affect their regulatory function on MGMT promoter activity and expression level. (Source: Cellular Oncology)
Source: Cellular Oncology - June 15, 2016 Category: Cancer & Oncology Source Type: research

Hypoxia can impair doxorubicin resistance of non-small cell lung cancer cells by inhibiting MRP1 and P-gp expression and boosting the chemosensitizing effects of MRP1 and P-gp blockers
Conclusions From our data we conclude that MRP1 and P-gp play critical roles in the DOX resistance of the NSCLC cells tested. MRP1 and P-gp targeted therapy using MK571, Verapamil, CoCl2 or ambient hypoxia appeared to be promising in abolishing the DOX efflux and DOX resistance of the NSCLC cells. The putative therapeutic efficacies of MRP1 and/or P-gp blockers on NSCLC cells are worthy of note. (Source: Cellular Oncology)
Source: Cellular Oncology - June 15, 2016 Category: Cancer & Oncology Source Type: research

The genetic heterogeneity of colorectal cancer predisposition - guidelines for gene discovery
Conclusions The picture emerges that the genetic predisposition to CRC is heterogeneous, involving complex interplays between common and rare (inter)genic variants with different penetrances. It is anticipated, however, that the use of large clinically well-defined patient and control datasets, together with improved functional and technical possibilities, will yield enough power to unravel this complex interplay and to generate accurate individualized estimates for the risk to develop CRC. (Source: Cellular Oncology)
Source: Cellular Oncology - June 9, 2016 Category: Cancer & Oncology Source Type: research

Weighted gene co-expression network analysis reveals key genes involved in pancreatic ductal adenocarcinoma development
Conclusions Using this new systems biology approach, we identified several genes that appear to be critical to PDAC development. As such, they may represent potential diagnostic biomarkers as well as therapeutic targets with clinical utility. (Source: Cellular Oncology)
Source: Cellular Oncology - May 30, 2016 Category: Cancer & Oncology Source Type: research

The role of lymphangiogenesis and angiogenesis in tumor metastasis
Conclusions Lymphangiogenesis and angiogenesis are important phenomena involved in the spread of cancer cells and they are associated with a poor prognosis. It is anticipated that new discoveries and advancing knowledge on these phenomena will allow an improvement in the treatment of cancer patients. (Source: Cellular Oncology)
Source: Cellular Oncology - April 28, 2016 Category: Cancer & Oncology Source Type: research

High glucose and insulin enhance uPA expression, ROS formation and invasiveness in breast cancer-derived cells
Conclusions From our data we conclude that hyperglycemia and hyperinsulinemia can promote breast cancer cell proliferation, migration and invasion. We found that these features were associated with increased expression of the mesenchymal markers vimentin and fibronectin, as well as increased uPA expression and activation through a mechanism mediated by ROS. (Source: Cellular Oncology)
Source: Cellular Oncology - April 22, 2016 Category: Cancer & Oncology Source Type: research

Non-coding RNAs in pancreatic cancer: challenges and opportunities for clinical application
Conclusions Although the number of reports addressing the role of ncRNAs in PDAC virtually grows by the day, there are still many steps to be taken before the application of ncRNA-based therapies will become reality in clinical practice. (Source: Cellular Oncology)
Source: Cellular Oncology - April 8, 2016 Category: Cancer & Oncology Source Type: research

TNF- α promotes breast cancer cell migration and enhances the concentration of membrane-associated proteases in lipid rafts
Conclusions From our results we conclude that TNF- α-induced activation of the MAPK/ERK signaling pathway may promote breast cancer cell migration via both upregulation of MMP9, CD26 and FAP-α and concentration of these proteases, as also MT1-MMP and MMP2, in the lipid rafts. TNF-α may serve as a potential therapeutic target in breast cancers sus ceptible to TNF-α stimulation. (Source: Cellular Oncology)
Source: Cellular Oncology - April 4, 2016 Category: Cancer & Oncology Source Type: research

TNF-α promotes breast cancer cell migration and enhances the concentration of membrane-associated proteases in lipid rafts
Conclusions From our results we conclude that TNF-α-induced activation of the MAPK/ERK signaling pathway may promote breast cancer cell migration via both upregulation of MMP9, CD26 and FAP-α and concentration of these proteases, as also MT1-MMP and MMP2, in the lipid rafts. TNF-α may serve as a potential therapeutic target in breast cancers susceptible to TNF-α stimulation. (Source: Cellular Oncology)
Source: Cellular Oncology - April 4, 2016 Category: Cancer & Oncology Source Type: research

Inactivation of CYLD in intestinal epithelial cells exacerbates colitis-associated colorectal carcinogenesis - a short report
Conclusions Our findings underscore a critical tumor-suppressing role for functional intestinal epithelial CYLD in colitis-associated carcinogenesis. CYLD expression and its associated pathways in intestinal tumors may be exploited for future prognostic and therapeutic purposes. (Source: Cellular Oncology)
Source: Cellular Oncology - April 4, 2016 Category: Cancer & Oncology Source Type: research

Giganteaside D induces ROS-mediated apoptosis in human hepatocellular carcinoma cells through the MAPK pathway
Conclusions From our results we conclude that GD can induce ROS-mediated apoptosis in HCC-derived cells through the MAPK pathway. This observation may open up avenues to explore the future use of GD as a HCC chemotherapeutic agent. (Source: Cellular Oncology)
Source: Cellular Oncology - March 25, 2016 Category: Cancer & Oncology Source Type: research

Schedule-dependent cytotoxicity of sunitinib and TRAIL in human non-small cell lung cancer cells with or without EGFR and KRAS mutations
Conclusion From our data we conclude that administration of sunitinib followed by TRAIL, as well as a simultaneous administration of both agents, serve as favorable treatment regimens for NSCLC-derived cells, irrespective of their EGFR and/or KRAS mutation status. (Source: Cellular Oncology)
Source: Cellular Oncology - March 25, 2016 Category: Cancer & Oncology Source Type: research

Altered primary chromatin structures and their implications in cancer development
Conclusions The primary chromatin structure is regulated by a variety of epigenetic mechanisms that may be deregulated through gene mutations and/or gene expression alterations. In recent years, it has become evident that changes in chromatin structure may coincide with the occurrence of cancer hallmarks. The functional interrelationships between such epigenetic alterations and cancer development are just becoming manifest and, therefore, the oncology community should continue to explore the molecular mechanisms governing the primary chromatin structure, both in normal and in cancer cells, in order to improv...
Source: Cellular Oncology - March 23, 2016 Category: Cancer & Oncology Source Type: research

Prognostic implications of securin expression and sub-cellular localization in human breast cancer
Abstract Purpose Securin belongs to a class of cell cycle regulators that prevent metaphase-to-anaphase transition until sister chromatid separation is complete. Evidence is accumulating that securin has a prognostic impact on a variety of malignancies but, thus far, the role and regulation of securin expression and its sub-cellular localization have not been systematically addressed in breast cancer. Methods In total 470 breast cancer specimens with follow-up data for up to 22 years were included. Immunohistochemical staining and immunofluorescence...
Source: Cellular Oncology - March 16, 2016 Category: Cancer & Oncology Source Type: research

Cytotoxicity of allitinib, an irreversible anti-EGFR agent, in a large panel of human cancer-derived cell lines: KRAS mutation status as a predictive biomarker
Conclusions The current study represents the largest in vitro assessment of allitinib cytotoxicity performed to date. Through this study, we identified cancer types that could potentially benefit from this drug. Additionally, our findings suggest that prevalent KRAS mutations constitute potential predictive biomarkers for allitinib response. (Source: Cellular Oncology)
Source: Cellular Oncology - February 26, 2016 Category: Cancer & Oncology Source Type: research

A novel curcumin-like dienone induces apoptosis in triple-negative breast cancer cells
Conclusion Our findings indicate that dienone 2c uses the mitochondrial/intrinsic pathway to inflict apoptosis in triple negative MDA-MB-231 breast cancer-derived cells. This observation warrants further assessment of dienone 2c as a potential anti-breast cancer drug. (Source: Cellular Oncology)
Source: Cellular Oncology - February 26, 2016 Category: Cancer & Oncology Source Type: research

Tumor-specific promoter-driven adenoviral therapy for insulinoma
Conclusions INSM1 promoter-driven conditionally replicating adenoviruses may serve as a new tool for the treatment of insulinoma and may provide clinicians with additional options to combat this disease. (Source: Cellular Oncology)
Source: Cellular Oncology - February 22, 2016 Category: Cancer & Oncology Source Type: research

Small-molecule inhibitors of FGFR, integrins and FAK selectively decrease L1CAM-stimulated glioblastoma cell motility and proliferation
Conclusions We here show for the first time that small-molecule inhibitors of FGFR, integrins and FAK effectively and selectively abolish L1-stimulated migration and proliferation of glioblastoma-derived cells. Our results suggest that these inhibitors have the potential to reduce the aggressiveness of high-grade gliomas expressing L1. (Source: Cellular Oncology)
Source: Cellular Oncology - February 16, 2016 Category: Cancer & Oncology Source Type: research

Melanoma associated antigen (MAGE)-A3 promotes cell proliferation and chemotherapeutic drug resistance in gastric cancer
Conclusions From our results we conclude that MAGE-A3 expression is regulated epigenetically by promoter methylation, and that its expression contributes to gastric cell proliferation and drug sensitivity. This study underscores the potential implications of MAGE-A3 as a therapeutic target and prognostic marker in gastric cancer patients. (Source: Cellular Oncology)
Source: Cellular Oncology - February 11, 2016 Category: Cancer & Oncology Source Type: research

Biomarkers for the identification of precursor polyps of colorectal serrated adenocarcinomas
Conclusion Based on the shared presence of protein and molecular biomarkers, especially FSCN1 expression, SSA may serve as a precursor lesion of SAC. Biomarker assessment may help in discerning colorectal carcinogenic routes with distinct prognostic implications. (Source: Cellular Oncology)
Source: Cellular Oncology - February 1, 2016 Category: Cancer & Oncology Source Type: research

PSA and beyond: alternative prostate cancer biomarkers
Conclusions With the advent of advanced genomic and proteomic technologies, we have in recent years seen an enormous spurt in prostate cancer biomarker research with several promising alternative biomarkers being discovered that show an improved sensitivity and specificity over PSA. The new generation of biomarkers can be tested via serum, urine, or tissue-based assays that have either received regulatory approval by the US Food and Drug Administration or are available as Clinical Laboratory Improvement Amendments-based laboratory developed tests. Additional emerging novel biomarkers for prostate cancer, includin...
Source: Cellular Oncology - January 20, 2016 Category: Cancer & Oncology Source Type: research

Simultaneous detection of circulating immunological parameters and tumor biomarkers in early stage breast cancer patients during adjuvant chemotherapy
Conclusions From our results we conclude that adjuvant chemotherapy suppresses immune function in early stage breast cancer patients. In addition, we conclude that the presence of circulating tumor cells, defined as pan-cytokeratin+, CD326+, CD45− cells, may serve as an important indicator of a patient’s immune status. Further investigations are needed to firmly define circulating tumor cells as a predictor for the success of breast cancer adjuvant chemotherapy. (Source: Cellular Oncology)
Source: Cellular Oncology - January 14, 2016 Category: Cancer & Oncology Source Type: research

Alternative splicing within the Wnt signaling pathway: role in cancer development
Conclusions Splice isoforms of components of the Wnt signaling pathway play distinct roles in cancer development. Isoforms of the same component may function in a tissue- and/or cancer-specific manner. Splice isoform expression analyses along with deregulated Wnt signaling pathway analyses may be of help to design efficient diagnostic and therapeutic strategies. (Source: Cellular Oncology)
Source: Cellular Oncology - January 13, 2016 Category: Cancer & Oncology Source Type: research

MiR-613 induces cell cycle arrest by targeting CDK4 in non-small cell lung cancer
Conclusions From our results we conclude that miR-613 may act as a tumor suppressor in NSCLC and may serve as a tool for miRNA-based NSCLC therapy. (Source: Cellular Oncology)
Source: Cellular Oncology - January 7, 2016 Category: Cancer & Oncology Source Type: research

Altered Lamin A/C splice variant expression as a possible diagnostic marker in breast cancer
Conclusions From our data we conclude that the Lamin C : Lamin A mRNA ratio is increased in breast cancer and that this mRNA ratio may be of diagnostic use in all clinical stages of breast cancer and, possibly, also in liver, lung, thyroid, colon, ovary and prostate cancers. (Source: Cellular Oncology)
Source: Cellular Oncology - January 5, 2016 Category: Cancer & Oncology Source Type: research

Human IGF1 pro-forms induce breast cancer cell proliferation via the IGF1 receptor
Conclusions Our data indicate that IGF1 pro-forms can induce breast cancer cell proliferation via the IGF1R, independent from the mature IGF1 form. These results underline the importance of an accurate assessment of the presence of IGF1 pro-forms within the breast cancer microenvironment. (Source: Cellular Oncology)
Source: Cellular Oncology - December 23, 2015 Category: Cancer & Oncology Source Type: research

Treatment of LS174T colorectal cancer stem-like cells with n-3 PUFAs induces growth suppression through inhibition of survivin expression and induction of caspase-3 activation
Conclusion From our data we conclude that PUFAs induce growth inhibition via targeting survivin expression in LS174T cells, which serve as a model for CCSCs. (Source: Cellular Oncology)
Source: Cellular Oncology - December 15, 2015 Category: Cancer & Oncology Source Type: research

HepG2 cells acquire stem cell-like characteristics after immune cell stimulation
Conclusions We conclude that the immune system may have a double-edge effect on cancer development. On one hand, immune cells such as B lymphocytes and macrophages may recognize, attack and eliminate cancer cells, whereas on the other hand, they may promote a subset of cancer cells to acquire stem cell-like characteristics. (Source: Cellular Oncology)
Source: Cellular Oncology - December 9, 2015 Category: Cancer & Oncology Source Type: research

Identification of miR-139-5p as a saliva biomarker for tongue squamous cell carcinoma: a pilot study
Conclusions From our results we conclude that saliva can be used as a feasible source for routine TSCC diagnostics and that miR-139-5p may serve as a potential biomarker for early TSCC detection. (Source: Cellular Oncology)
Source: Cellular Oncology - December 9, 2015 Category: Cancer & Oncology Source Type: research

Down-regulated ECRG4 expression in breast cancer and its correlation with tumor progression and poor prognosis - A short Report
Conclusion Our data suggest that loss of ECRG4 protein expression may be involved in tumor progression and may serve as a prognostic biomarker for breast cancer. (Source: Cellular Oncology)
Source: Cellular Oncology - December 2, 2015 Category: Cancer & Oncology Source Type: research

Efficacy of doxorubicin-transferrin conjugate in apoptosis induction in human leukemia cells through reactive oxygen species generation
Conclusions Our data indicate that DOX-TRF is considerably more cytotoxic to human leukemia cells than free DOX. In addition, we show that DOX-TRF can effectively produce free radicals, which are directly involved in apoptosis induction. (Source: Cellular Oncology)
Source: Cellular Oncology - November 26, 2015 Category: Cancer & Oncology Source Type: research

Efficacy of sorafenib correlates with Memorial Sloan-Kettering Cancer Center (MSKCC) risk classification and bone metastasis in Chinese patients with metastatic renal cell carcinoma
The objective of this single center, single arm retrospective study was to examine the efficacy and safety of sorafenib and its related prognostic clinico-pathologic factors in Chinese mRCC patients. Methods One hundred thirty four mRCC patients were enrolled. All patients received 400 mg of sorafenib orally twice daily. The dose was subsequently adjusted in the event of treatment-induced toxicity. Tumor response, progression-free survival (PFS), overall survival (OS) and adverse events (AEs) were determined. Results The median PFS and OS ...
Source: Cellular Oncology - November 23, 2015 Category: Cancer & Oncology Source Type: research

PDCD2 and NCoR1 as putative tumor suppressors in gastric gastrointestinal stromal tumors
Conclusions Our data indicate that both PDCD2 and NCoR1 may act as tumor suppressors in GIST cells through the Smad signaling pathway. (Source: Cellular Oncology)
Source: Cellular Oncology - November 20, 2015 Category: Cancer & Oncology Source Type: research

Down-regulation of osteopontin mediates a novel mechanism underlying the cytostatic activity of TGF-β
Conclusions Our results indicate that the OPN gene is a direct target of Smad-mediated TGF-β signaling, implying that OPN expression inhibition serves as a novel mechanism underlying the cytostatic activity of TGF-β. (Source: Cellular Oncology)
Source: Cellular Oncology - November 19, 2015 Category: Cancer & Oncology Source Type: research

Growth-suppressive effect of suberoylanilide hydroxamic acid (SAHA) on human oral cancer cells
Conclusions Our results indicate that the ERK signaling pathway may constitute a critical denominator of SAHA-induced apoptosis in OSCC-derived cells and that SAHA may have therapeutic potential for OSCC. (Source: Cellular Oncology)
Source: Cellular Oncology - November 18, 2015 Category: Cancer & Oncology Source Type: research

Lamin A/C deficiency is an independent risk factor for cervical cancer
Conclusion Lamin A/C deficiency may serve as an independent risk factor for CIN development and as an indicator for preventive therapy in cervical cancer. (Source: Cellular Oncology)
Source: Cellular Oncology - November 4, 2015 Category: Cancer & Oncology Source Type: research

DBD-F induces apoptosis in gastric cancer-derived cells through suppressing HIF2α expression
Conclusions From our findings we conclude that DBD-F (i) is cytotoxic to gastric cancer-derived cells and (ii) can induce apoptosis in these cells via the MEK/ERK signaling pathway. In addition, our findings strongly indicate that DBD-F can inhibit HIF2α expression by affecting the phosphorylation status of MEK/ERK in gastric cancer-derived cells. (Source: Cellular Oncology)
Source: Cellular Oncology - November 2, 2015 Category: Cancer & Oncology Source Type: research