Deciphering the metabolic heterogeneity of hematopoietic stem cells with single-cell resolution
Cell Metab. 2024 Jan 2;36(1):209-221.e6. doi: 10.1016/j.cmet.2023.12.005.ABSTRACTMetabolic status is crucial for stem cell functions; however, the metabolic heterogeneity of endogenous stem cells has never been directly assessed. Here, we develop a platform for high-throughput single-cell metabolomics (hi-scMet) of hematopoietic stem cells (HSCs). By combining flow cytometric isolation and nanoparticle-enhanced laser desorption/ionization mass spectrometry, we routinely detected >100 features from single cells. We mapped the single-cell metabolomes of all hematopoietic cell populations and HSC subpopulations with differ...
Source: Cell Metabolism - January 3, 2024 Category: Cytology Authors: Jing Cao Qi Jason Yao Jiao Wu Xiaonan Chen Lin Huang Wanshan Liu Kun Qian Jing-Jing Wan Bo O Zhou Source Type: research
Mitochondrial-derived vesicles in metabolism, disease, and aging
Cell Metab. 2024 Jan 2;36(1):21-35. doi: 10.1016/j.cmet.2023.11.014.ABSTRACTMitochondria are central hubs of cellular metabolism and are tightly connected to signaling pathways. The dynamic plasticity of mitochondria to fuse, divide, and contact other organelles to flux metabolites is central to their function. To ensure bona fide functionality and signaling interconnectivity, diverse molecular mechanisms evolved. An ancient and long-overlooked mechanism is the generation of mitochondrial-derived vesicles (MDVs) that shuttle selected mitochondrial cargoes to target organelles. Just recently, we gained significant insight i...
Source: Cell Metabolism - January 3, 2024 Category: Cytology Authors: Tim K önig Heidi M McBride Source Type: research
The DEAD Box Protein Mrh4 Functions in the Assembly of the Mitochondrial Large Ribosomal Subunit
Cell Metab. 2024 Jan 2;36(1):222. doi: 10.1016/j.cmet.2023.11.016.NO ABSTRACTPMID:38171336 | DOI:10.1016/j.cmet.2023.11.016 (Source: Cell Metabolism)
Source: Cell Metabolism - January 3, 2024 Category: Cytology Authors: Dasmanthie De Silva Flavia Fontanesi Antoni Barrientos Source Type: research
H < sub > 2 < /sub > S serves as the immunoregulatory essence of apoptotic cell death
Cell Metab. 2024 Jan 2;36(1):3-5. doi: 10.1016/j.cmet.2023.12.006.ABSTRACTApoptosis supports tissue homeostasis and prevents immune disorders by removing damaged and functionally aberrant cells. Here, Ou et al. utilized genetic, pharmacological, and proteomic approaches focused on sulfur amino acid catabolism to discover that hydrogen sulfide (H2S) release during apoptosis suppresses Th17 cell differentiation, thus providing therapeutic targets for autoimmune diseases.PMID:38171337 | DOI:10.1016/j.cmet.2023.12.006 (Source: Cell Metabolism)
Source: Cell Metabolism - January 3, 2024 Category: Cytology Authors: Christopher Hine Andr ás K Ponti Mar ía Ángeles Cáliz-Molina Alejandro Mart ín-Montalvo Source Type: research
Show MERCI on mobile mitochondria
Cell Metab. 2024 Jan 2;36(1):5-7. doi: 10.1016/j.cmet.2023.12.017.ABSTRACTThere is emerging evidence that mitochondria can move between cells, particularly from immune cells into cancers. Recent work from Zhang et al. in Cancer Cell employs single-cell RNA- and mitochondrial DNA-sequencing in co-culture experiments and patient tumor samples to detect mitochondrial transfer. However, the mechanisms, scale, and implications remain uncertain.PMID:38171338 | DOI:10.1016/j.cmet.2023.12.017 (Source: Cell Metabolism)
Source: Cell Metabolism - January 3, 2024 Category: Cytology Authors: Jelle van den Ameele Patrick F Chinnery Source Type: research
What we talk about when we talk about spinal cord aging
Cell Metab. 2024 Jan 2;36(1):7-9. doi: 10.1016/j.cmet.2023.12.002.ABSTRACTSpinal cord-associated disorders are common in the elderly population; however, the mechanisms underlying spinal aging remain elusive. In a recent Nature paper, Sun et al. systemically analyzed aged spines in nonhuman primates and identified a new cluster of CHIT1-positive microglia that drives motor neuron senescence and subsequent spine aging.PMID:38171339 | DOI:10.1016/j.cmet.2023.12.002 (Source: Cell Metabolism)
Source: Cell Metabolism - January 3, 2024 Category: Cytology Authors: Xianhong Ji Jiajia Zhang Xiaoqiang Tang Hou-Zao Chen Source Type: research
Repression of latent NF- κB enhancers by PDX1 regulates β cell functional heterogeneity
Cell Metab. 2024 Jan 2;36(1):90-102.e7. doi: 10.1016/j.cmet.2023.11.018.ABSTRACTInteractions between lineage-determining and activity-dependent transcription factors determine single-cell identity and function within multicellular tissues through incompletely known mechanisms. By assembling a single-cell atlas of chromatin state within human islets, we identified β cell subtypes governed by either high or low activity of the lineage-determining factor pancreatic duodenal homeobox-1 (PDX1). β cells with reduced PDX1 activity displayed increased chromatin accessibility at latent nuclear factor κB (NF-κB) enhancers. Pdx1 ...
Source: Cell Metabolism - January 3, 2024 Category: Cytology Authors: Benjamin J Weidemann Biliana Marcheva Mikoto Kobayashi Chiaki Omura Marsha V Newman Yumiko Kobayashi Nathan J Waldeck Mark Perelis Louise Lantier Owen P McGuinness Kathryn Moynihan Ramsey Roland W Stein Joseph Bass Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2024 Jan 2;36(1):62-77.e8. doi: 10.1016/j.cmet.2023.11.013. Epub 2023 Dec 21.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing the creatine...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2023 Dec 14:S1550-4131(23)00445-X. doi: 10.1016/j.cmet.2023.11.013. Online ahead of print.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2023 Dec 14:S1550-4131(23)00445-X. doi: 10.1016/j.cmet.2023.11.013. Online ahead of print.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2023 Dec 14:S1550-4131(23)00445-X. doi: 10.1016/j.cmet.2023.11.013. Online ahead of print.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2023 Dec 14:S1550-4131(23)00445-X. doi: 10.1016/j.cmet.2023.11.013. Online ahead of print.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2023 Dec 14:S1550-4131(23)00445-X. doi: 10.1016/j.cmet.2023.11.013. Online ahead of print.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2023 Dec 14:S1550-4131(23)00445-X. doi: 10.1016/j.cmet.2023.11.013. Online ahead of print.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth
Cell Metab. 2023 Dec 14:S1550-4131(23)00445-X. doi: 10.1016/j.cmet.2023.11.013. Online ahead of print.ABSTRACTGlioblastoma (GBM) is a malignancy dominated by the infiltration of tumor-associated myeloid cells (TAMCs). Examination of TAMC metabolic phenotypes in mouse models and patients with GBM identified the de novo creatine metabolic pathway as a hallmark of TAMCs. Multi-omics analyses revealed that TAMCs surround the hypoxic peri-necrotic regions of GBM and express the creatine metabolic enzyme glycine amidinotransferase (GATM). Conversely, GBM cells located within these same regions are uniquely specific in expressing...
Source: Cell Metabolism - December 22, 2023 Category: Cytology Authors: Aida Rashidi Leah K Billingham Andrew Zolp Tzu-Yi Chia Caylee Silvers Joshua L Katz Cheol H Park Suzi Delay Lauren Boland Yuheng Geng Steven M Markwell Crismita Dmello Victor A Arrieta Kaylee Zilinger Irene M Jacob Aurora Lopez-Rosas David Hou Brandyn Cas Source Type: research
