SPATA33 affects the formation of cell adhesion complex by interacting with CTNNA3 in TM4 cells
In this study, we found that SPATA33 and CTNNA3 were involved in this process. Quantitative real-time PCR and western blotting showed similar trend of expression of two proteins in the testis of mice of different ages. Subsequently, CRISPR-Cas9 technique was used to prepareSpata33 knockout cell lines with TM4 cells, cell wound scratch assay showed thatSpata33 gene knockout affected cell migration, and flow cytometry assay showed thatSpata33 knockout resulted in a decreased percentage of G1 phase cells in TM4 cell line. In addition, phalloidin staining assay showed thatSpata33 gene knockout disrupted the formation of F-acti...
Source: Cell and Tissue Research - May 10, 2022 Category: Cytology Source Type: research
Mesenchymal stem cell treatment for enteric neuropathy in the Winnie mouse model of spontaneous chronic colitis
AbstractInflammatory bowel disease (IBD) is a chronic gut inflammation with periods of acute flares and remission. Beneficial effects of a single dose of mesenchymal stem cell (MSC)-based treatment have been demonstrated in acute models of colitis. No studies investigated therapeutic effects of MSCs for the attenuation of enteric neuropathy in a chronic model of colitis. The short and long-term effects of MSC treatment in modulating inflammation and damage to the enteric nervous system (ENS) were studied in theWinnie mouse model of spontaneous chronic colitis highly representative of human IBD.Winnie mice received a single...
Source: Cell and Tissue Research - May 10, 2022 Category: Cytology Source Type: research
SPATA33 affects the formation of cell adhesion complex by interacting with CTNNA3 in TM4 cells
In this study, we found that SPATA33 and CTNNA3 were involved in this process. Quantitative real-time PCR and western blotting showed similar trend of expression of two proteins in the testis of mice of different ages. Subsequently, CRISPR-Cas9 technique was used to prepareSpata33 knockout cell lines with TM4 cells, cell wound scratch assay showed thatSpata33 gene knockout affected cell migration, and flow cytometry assay showed thatSpata33 knockout resulted in a decreased percentage of G1 phase cells in TM4 cell line. In addition, phalloidin staining assay showed thatSpata33 gene knockout disrupted the formation of F-acti...
Source: Cell and Tissue Research - May 10, 2022 Category: Cytology Source Type: research
Mesenchymal stem cell treatment for enteric neuropathy in the Winnie mouse model of spontaneous chronic colitis
AbstractInflammatory bowel disease (IBD) is a chronic gut inflammation with periods of acute flares and remission. Beneficial effects of a single dose of mesenchymal stem cell (MSC)-based treatment have been demonstrated in acute models of colitis. No studies investigated therapeutic effects of MSCs for the attenuation of enteric neuropathy in a chronic model of colitis. The short and long-term effects of MSC treatment in modulating inflammation and damage to the enteric nervous system (ENS) were studied in theWinnie mouse model of spontaneous chronic colitis highly representative of human IBD.Winnie mice received a single...
Source: Cell and Tissue Research - May 10, 2022 Category: Cytology Source Type: research
