Red blood cell distribution width and haemoglobin are associated with hospital admission in patients with acute allergic reactions.
This study aimed to investigate whether RDW value at admission may predict the need of hospitalisation in patients presenting to the emergency department (ED) with acute allergic reactions. MATERIALS AND METHODS: The study population consisted of adult patients (aged > 17) admitted to the ED for acute allergic reactions. RESULTS: One hundred and thirty-two subjects were included, 12 of whom (9%) required hospital admission for severity of symptoms. Patients who needed hospital admission displayed significantly lower values of haemoglobin and significantly higher values of RDW-coefficient of variation (...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Reduced penicillin susceptibility of group B Streptococcus: an assessment of emergence in Grampian, Scotland.
Authors: Cooper K, Abbott F, Gould IM PMID: 27182673 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Fine needle aspiration cytology for lymph nodes: a three-year study.
Authors: Zhou J, Li F, Meng L, Hao F, Liu X, Zhao C, Zhang K, Dong A Abstract BACKGROUND AND AIMS: The cytologic patterns of lymph node fine needle aspirations (FNAs) exhibit a wide variation in different diseases and in different ethnic groups in various geographical locations. Knowledge of lymphadenopathy patterns in a given geographical region is essential for making a confident diagnosis of suspected disease in that location. In the present study, we assessed the cytologic patterns of lymph node aspirations in patients in the Huangdao region of China. METHODS: A three-year retrospective study design was con...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Value of Ki-67 and computed tomography in the assessment of peripheral lung adenocarcinoma.
This study was designed to determine whether proliferation antigen Ki-67 and/or a computed tomography (CT) value could be used to evaluate the clinical-pathological features of peripheral lung adenocarcinoma. MATERIALS AND METHODS: A total of 116 eligible lung cancer patients were enrolled. Nodule size, lymph node metastasis, differentiation, Ki-67 expression and CT findings were assessed. The relationship between clinic parameters and the CT feature was analysed statistically. RESULTS: The percentage of lesions that had ground-glass opacity or localised air bronchogram was significantly greater in low CT value group (
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Intrafamilial transmission of Helicobacter pylori: genotyping of faecal samples.
CONCLUSIONS: In conclusion, mother-to-child transmission is the main route of intrafamilial transmission of H. pylori in Iranian families. Molecular typing of H. pylori can be useful in identifying a high-risk population. PMID: 27182676 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Antifungal effect of Echinophora platyloba on expression of CDR1 and CDR2 genes in fluconazole-resistant Candida albicans.
Authors: Khajeh E, Hosseini Shokouh SJ, Rajabibazl M, Roudbary M, Rafiei S, Aslani P, Farahnejad Z Abstract BACKGROUND: Several studies examined the effect of the Echinophora platyloba extract in treatment of azole-resistant Candida albicans clinical isolates. OBJECTIVE: We investigated the effect of E. platyloba extract on expression of CDR1 and CDR2 genes in fluconazole-resistant clinical isolates of C. albicans using real-time PCR. MATERIALS AND METHODS: The crude extract of E. platyloba was obtained using percolation method. Using serial dilution method, different concentrations of extract were achieved...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Letter to the Editor: Antimicrobial properties of mucus from the brown garden snail Helix aspersa.
Authors: Bortolotti D, Trapella C, Bernardi T, Rizzo R PMID: 27182678 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Effects of serum indices interference on hormonal results from the Abbott Architect i2000 immunoassay analyser.
In this study, we assess the impact of interferences from exogenous serum indices on various endocrine assays performed on the Abbott Architect i2000 system. The pool of 20 serum samples was derived from a hospitalised population. The diluted serum samples were spiked with red cell haemolysate, Intralipid and bilirubin. The interferences were studied at baseline; 12.5%, 25%, 50%, 75% and 100% of 5.0 g/L haemoglobin; 1% of 20% Intralipid; and 0.342 mmol/L of bilirubin according to the EP7-A2 guideline (Interference Testing in Clinical Chemistry; CLSI, USA). Aliquots were analysed in duplicate and/or triplicate for various h...
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Pentraxin-3 and nitric oxide as indicators of disease severity in alcoholic cirrhosis.
Authors: Nandeesha H, Rajappa M, Manjusha J, Ananthanarayanan PH, Kadhiravan T, Harichandrakumar KT Abstract Recent studies have indicated that pentraxin-3 can be used as a marker to assess the severity of hepatic fibrosis in non-alcoholic steatohepatitis. The present study was designed to assess pentraxin-3, nitric oxide and tumour necrosis factor-α (TNFα) in alcoholic cirrhosis and their association with disease severity. We enrolled 47 alcoholic cirrhosis cases and 32 controls. Serum pentraxin-3, nitric oxide (NO) and TNFα levels were estimated in both groups. Serum pentraxin-3, NO and TNF&alph...
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

No role for tri-iodothyronine (T3) testing in the assessment of levothyroxine (T4) over-replacement in hypothyroid patients.
This study investigated how informative T3 levels are in these patients. Our hypothesis is that T3 measurement would not add anything to the assessment of T4 over-replacement in primary hypothyroidism. Over a 15-week period, consecutive thyroid function test requests in patients on levothyroxine had T3 levels measured if thyroid-stimulating hormone (TSH) was below the reference range (RR;
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation of antioxidant status of female diabetic patients in Nnamdi Azikiwe University Teaching Hospital, Anambra State, Nigeria.
This study aims to evaluate the level of antioxidant status in type 2 diabetes mellitus (DM) female participants visiting the out-patient diabetic clinic of Nnamdi Azikiwe University Teaching Hospital (NAUTH), Nnewi, Anambra State, Nigeria. A total of 86 participants aged 51±10 years were recruited for this study. The test group consists of 43 already confirmed type 2 diabetes mellitus females, while the control group consists of 43 apparently healthy females. The test subjects were further subgrouped into good and poor glycaemic control groups, using a cut-off of
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

TNFAIP3 and TNIP1 polymorphisms confer psoriasis risk in South Indian Tamils.
Authors: Indhumathi S, Rajappa M, Chandrashekar L, Ananthanarayanan PH, Thappa DM, Negi VS Abstract Psoriasis is a chronic inflammatory skin disease with genetic and environmental factors having an important role in its aetiology. Several genome-wide association studies have reported the association of the genes of the TNFα signalling, tumour necrosis factor alpha-induced protein 3 (TNFAIP3), TNFAIP3-interacting protein 1 (TNIP1) with psoriasis in Western and Chinese populations. The aim of this study is to demonstrate whether the TNFAIP3 and TNIP1 genes contribute to the risk of psoriasis in the ethnically d...
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Antimicrobial properties of mucus from the brown garden snail Helix aspersa.
This study sought to investigate the antimicrobial properties of H. aspersa mucus. Mucus was collected from H. aspersa snails, diluted in phosphate-buffered saline (PBS), with the supernatant tested against a wide range of organisms in a disc-diffusion antimicrobial assay. This was followed with comparative experiments involving A. fulica, including bacteriophage assays. Mucus from both species of snail was passed through a series of protein size separation columns in order to determine the approximate size of the antimicrobial substance. Electrophoresis was also carried out on the H. aspersa mucus. Results indicated that ...
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Enterococcus faecalis as multidrug resistance strains in clinical isolates in Imam Reza Hospital in Kermanshah, Iran.
In conclusion, a high rate of resistance to antibiotics in Enterococcus is clearly problematic, and a novel strategy is needed to decrease resistance in Enterococcus. PMID: 26738400 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Audit and review for evidence-based red cell wastage reduction measures.
Authors: Smith GA, Gopal-Patel J, Joseph JV, Hobson A, Clarke K Abstract Stocks of red blood cells (RBC) are held to ideally match supply and demand; hold too great a stock and unnecessary wastage occurs; too low a stock results in delay or lack of blood for the patient. Blood is a precious resource and its supply needs to be managed effectively. The aim was to identify how RBC units are wasted and propose laboratory-based reduction measures that would not compromise the clinical requirements of the patient. Wastage of RBC was investigated using a 'dashboard' query of a laboratory information management system. By ...
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A post-antibiotic era looms: can plant natural product research fill the void?
Authors: Kenny CR, Furey A, Lucey B Abstract Antimicrobial resistance is increasing among certain pathogenic bacteria to the extent that treatment efficacy is no longer always assured. According to the CDC, as few as six new antibiotics have been released for use over the past 30 years. Resistance has already been observed to each of these. Eleven plant natural products have been approved for therapeutic use during the same period--none of them being antimicrobial agents. We have learned through experience that some microorganisms will inevitably overcome antibiotic treatment in certain situations, and then spread....
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Low serum creatine kinase activity in hospital patients.
Authors: Cobbold L, Curd R, Crook MA PMID: 26738403 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Bacteraemia caused by Fusobacterium necrophorum: a clinical approach to Lemierre's syndrome.
Authors: Lee JH, Yang TU, Lee HW, Yoon SY, Lim CS PMID: 26738404 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

How reliable are cough swabs for the detection of Pseudomonas aeruginosa in adult patients with cystic fibrosis?
Authors: Moore JE, McCaughan J, McNeilly J, Rendall JC, Downey DG PMID: 26738405 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 9, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Tissue fatty acid composition in human urothelial carcinoma.
Authors: Miryaghoubzadeh J, Darabi M, Madaen K, Shaaker M, Mehdizadeh A, Hajihosseini R Abstract Bladder cancer cells appear to have an altered lipid metabolism as evidenced by modulated lipogenic enzymes. The aim of this study is to investigate differences in tissue fat composition between malignant and adjacent normal urinary bladder tissue. Normal-appearing and malignant bladder tissues were collected from 31 patients with high-grade (Ta) urothelial carcinoma during transurethral resection (TUR). The fatty acid composition in the tissue was determined by gas liquid chromatography. In the bladder cancer tissue, l...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Brand of dipotassium EDTA vacuum tube as a new source of pre-analytical variability in routine haematology testing.
This study assesses the use of different dry K2 (dipotassium) EDTA vacuum tubes and whether or not they might represent a bias in haematological testing. Blood was collected in three dipotassium EDTA vacuum tubes from different manufacturers: Venosafe, Vacuette and Vacutainer. Samples were analysed on an Advia 2120i analyser. Significant differences among results and biases were compared with current quality specifications. Significant differences were found for haematocrit (HCT), mean corpuscular volume (MCV), white blood cell count (WBC) and platelet distribution width (PDW) when comparing Venosafe vs. Vacuette; for MCV,...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Clinical significance of autoantibodies directed against nuclear and cytoplasmic antigens in autoimmune liver disease.
This study aims to facilitate a consensus on these autoantibodies in an unselected patient population and their relationship to autoimmune liver disease (AILD). The study detected two autoantibodies that show reasonable specificity for primary biliary cirrhosis (PBC), anti-sp100 and anti-gp210, and it may be clinically useful to report any antimitochondrial antibody (AMA) detected, as this may be a sign of very early PBC. The inter-methodological differences in assays available for detection of the autoantibodies were also noted. Care must be taken when selecting methods to detect these autoantibodies. PMID: 23617092 [...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Construction, expression and characterisation of recombinant molecules of the urease gene operon from a urease-positive thermophilic Campylobacter (UPTC) isolate.
Authors: Nakanishi S, Nakajima T, Tazumi A, Matsubara K, Moore JE, Millar BC, Matsuda M Abstract A recombinant molecule of the full-length urease gene operon was constructed in vitro from the Japanese urease-positive thermophilic Campylobacter (UPTC) CF89-12 isolate and expressed in Escherichia coli cells. Several large deletion recombinant variants of urease subunit genes were also constructed and expressed in E. coli cells. A positive urease reaction with the log-phase cultured E. coli JM109 cells in the NiCl2-containing medium transformed with pGEM-T vector carrying the recombinant molecule of the full-length op...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The FUS1 gene inhibits EC109 cell growth mediated by a lentivirus vector.
Authors: Zhang B, Xu X, Qi Z, Peng L, Qiu B, Huo X Abstract The effects of the FUS1 gene on the oesophageal carcinoma cell line EC109 are investigated. The messenger RNA (mRNA) expression level of the FUS1 gene was detected by a reverse transcription polymerase chain reaction (RT-PCR) technique in the cell lines SHEE, SHEEC and EC109. The full length of the FUS1 gene was amplified using a PCR technique from the total RNA of umbilical mesenchymal stem cells. The FUS1 gene was cloned into a pSL6-IRES-EGFP vector and identified by PCR, digestion and sequencing. The recombinant pSL6-FUS1-IRES-EGFP plasmid was transfect...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Seroprevalence of hepatitis E and Helicobacter pylori in a low socioeconomic area of a metropolitan city in a developing country.
This study aims to determine the prevalence of coinfection of H. pylori and hepatitis E virus (HEV) in the paediatric age group in an urban slum area of Karachi and identify risk factors associated with co-infection. Five hundred and forty children aged one to 15 years were investigated. Blood samples were collected and questionnaires completed on socio-demographic characteristics. Anti-H. pylori, HEV IgG and IgM antibodies were analysed by enzyme immunoassays (EIAs). The seroprevalence of H. pylori antibody was 47.2%, while that of HEV IgG and IgM was 14.4% and 2.4%, respectively. 12.4% exhibited seroprevalence for both H...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

DNA damage repair and tolerance: a role in chemotherapeutic drug resistance.
Authors: Salehan MR, Morse HR Abstract A significant barrier to effective cancer therapy is the development of resistance to the drugs utilised. Standard chemotherapeutic regimens typically contain genotoxic agents, designed to damage DNA of existing tumour cells as well as prevent the synthesis of new DNA during proliferation. DNA damage in normal cells can be repaired efficiently or tolerated to preserve cellular and organ functionality. The mechanisms of DNA repair and tolerance are distinct for different types of lesion, but can be predicted if the mechanism of interaction of the drug with the DNA is known. The...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Comparative evaluation of chromID MRSA agar and Brilliance 2 MRSA agar for detection of MRSA in clinical samples.
Authors: McElhinney R, Millar C, Scopes E PMID: 23617097 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Observations on the number of saliva cotinine positives over a nine-year period.
Authors: Mortlock S, McLean F, Jones E, Willis S PMID: 23617098 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Role of carcinoembryonic antigen as a marker for colorectal liver metastases.
This study aims to determine the accuracy of CEA in the detection and resectability of colorectal liver metastases. Patients with primary colorectal cancer were divided into three groups: resectable hepatic metastases (group 1), unresectable metastases (group 2), and disease-free cases (group 3). The CEA concentration was recorded pre- and post-hepatectomy in group 1 and on radiological confirmation of disease state in the other groups. It was expressed as median (95% confidence interval [CI]), with predictors of concentration determined. Group 1 (n=141) had pre-operative CEA of 8.9 (4.6-13.1), with 38.1% of patients being...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Chlamydia trachomatis: TLR4-mediated recognition by human dendritic cells is impaired following oestradiol treatment.
Authors: Agrawal T, Bhengraj AR, Vats V, Mittal A Abstract Genital Chlamydia trachomatis infection creates a substantial reproductive health burden in women. The high incidence of asymptomatic infection often precludes timely antibiotic therapy to control the sequelae of infection, and therefore a vaccine is required. Dendritic cells (DC) are now being used as an adjuvant for vaccine development; however, the fate of C. trachomatis in human DC and differential regulation of cytokine secretion remains unclear. Hence, an in vitro study was performed using C. trachomatis (serovar D) elementary body (EB)-pulsed, monocy...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Exposure of vancomycin-sensitive Staphylococcus aureus to subinhibitory levels of vancomycin leads to upregulated capsular gene expression.
In this study, a vancomycin-susceptible S. aureus (VSSA) strain, which was methicillin-resistant (MRSA), was grown with and without subinhibitory levels of vancomycin, and the transcriptional profiles were determined by microarray analysis. Thirty-six genes were upregulated and 42 genes were down-regulated by more than two-fold (P
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Molecular test to determine toxigenic capabilities in GDH-positive, toxin-negative samples: evaluation of the Portrait toxigenic C. difficile assay.
This study aims to assess the introduction of the Portrait C. difficile assay as the third step to identify the presence of the toxigenic C. difficile B (tcdB) gene and thus determine toxigenic capability. Stool samples with a GDH-positive, toxin-negative result were tested using the Portrait analyser to detect the presence of tcdB. A retrospective evaluation was performed, assessing the clinical course of patients who were isolated as a result of the current algorithm using GDH enzyme immunoassay (EIA) and toxin EIA. Of the stool samples tested, 40% carried the tcdB gene. Four tcdB-positive stool samples initially toxin A...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation of the QBC Star centrifugal three-part differential haematology system.
Authors: Erhabor O, Richardson G, Mohammed I, Thornton C, Bark J, Hurst M, Hamer D, Kinsella P Abstract The QBC Star haematology system includes the QBC Star centrifugal analytical analyser and the QBC Star tube system. Together, they are capable of producing a haematology profile on venous or capillary whole blood. The aim of this study is to compare full blood count (FBC) including differential white cell count performance between the QBC Star analyser and a gold standard Sysmex XE-2100 haematology analyser. The FBC performance was evaluated according to the National Committee for Clinical Laboratory Standards (N...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Glutathione in multiple sclerosis.
Authors: Ferreira B, Mendes F, Osório N, Caseiro A, Gabriel A, Valado A Abstract Multiple sclerosis is a chronic inflammatory disease of the central nervous system, characterised mainly as an autoimmune neurodegenerative disorder. Its cause is unknown but multifactorial; however, some studies suggest that oxidative stress may be one of the sources, or a consequence of the disease, from loss of oxidant/antioxidant balance. This review studies glutathione, one of the most important agents of the endogenous antioxidant defence system, protecting cells from damage caused by oxidative stress. It evaluates glutath...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Efficacy of the biocide Steri-7 against the common Gram-negative bacterial pathogens (Burkholderia cenocepacia, Burkholderia gladioli, Burkholderia multivorans, Pseudomonas aeruginosa and Stenotrophomonas maltophilia) associated with cystic fibrosis (CF).
Authors: Moore JE, Rao JR PMID: 23888610 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Antibiotic resistance reversal (ARR) in Gram-negative and Gram-positive pathogens employing electric fields.
Authors: Nakanishi S, Rao JR, Matsuda M, Goldsmith CE, Coulter WA, Moore JE PMID: 23888611 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

OPathPaed service model for expanded newborn screening in Hong Kong SAR, China.
Authors: Mak CM, Lam C, Siu W, Law C, Chan W, Lee HC, Chong Y, Chen SP, Ching C, Wong C, Lee M, Chow K, Lee K, Chan W, Chan K, Lee SY, Chan YW PMID: 23888612 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Prevalence of Salmonella species among asymptomatic food handlers in Khartoum State, Sudan.
Authors: Ibrahim SA, Asakir SF, Idris AA, Martinez-Urtaza J, Hag Elsafi HE PMID: 23888613 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Detecting methicillin resistance in Staphylococcus aureus: comparison of different phenotypic methods and the polymerase chain reaction.
Authors: Ahmad S Abstract Cefoxitin is a more potent inducer of the mecA regulatory system compared to oxacillin in Staphylococcus aureus. It has been recommended for the detection of methicillin-resistant Staphylococcus aureus (MRSA) when using disk-diffusion testing. The aim of this study is to compare the results of cefoxitin and oxacillin disk-diffusion methods against the polymerase chain reaction (PCR) for the detection of MRSA. A total of 75 strains of S. aureus isolated from different clinical specimens (e.g., pus, blood, wound swabs, tracheal aspirates, eye swabs and cerebrospinal fluid [CSF]) were screene...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Arterial blood gas analysers: accuracy in determining haemoglobin, glucose and electrolyte concentrations in critically ill adult patients.
This study aims to assess the concordance of ABG machines in analysing haemoglobin, glucose and electrolyte concentrations compared to standard venous analysers as the gold standard. Results from 100 patients undergoing ABG analysis and simultaneous venous sampling without therapeutic intervention between sampling were compared. Differences in haemoglobin, glucose, sodium and potassium concentrations were determined and analysed using statistical software (Statview). There was a significant difference (P
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Cerebrospinal fluid ferritin in children with viral and bacterial meningitis.
Authors: Rezaei M, Mamishi S, Mahmoudi S, Pourakbari B, Khotaei G, Daneshjou K, Hashemi N Abstract Despite the fact that the prognosis of bacterial meningitis has been improved by the influence of antibiotics, this disease is still one of the significant causes of morbidity and mortality in children. Rapid differentiation between bacterial and aseptic meningitis, and the need for immediate antibiotic treatment in the former, is crucial in the prognosis of these patients. Ferritin is one of the most sensitive biochemical markers investigated in cerebrospinal fluid (CSF) for the early diagnosis of bacterial meningiti...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Cytokine gene polymorphisms of TNFα, IL-6, IL-10, TGFβ and IFNγ in the Saudi population.
Cytokine gene polymorphisms of TNFα, IL-6, IL-10, TGFβ and IFNγ in the Saudi population. Br J Biomed Sci. 2013;70(3):104-9 Authors: Alhamad EH, Cal JG, Shakoor Z, Almogren A Abstract Studies have demonstrated associations between cytokine gene polymorphisms and ethnicity. In the present work the authors examine polymorphisms in the genes encoding interleukin-6 (IL-6), IL-10, interferon-gamma (IFNgamma), tumour necrosis factor-alpha (TNFalpha) and transforming growth factor-beta (TGFbeta1) using the polymerase chain reaction sequence-specific primer (PCR-SSP) method in 150 healthy unrel...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Multiple myeloma: the bone marrow microenvironment and its relation to treatment.
Authors: Andrews SW, Kabrah S, May JE, Donaldson C, Morse HR Abstract Multiple myeloma is the most common haematological malignancy yet currently it remains incurable. For decades the mainstay in therapy has been non-targeted approaches including genotoxic agents and immunosuppressants. With myeloma predominantly affecting an elderly population, who are vulnerable to aggressive therapy, these non-specific approaches have resulted in poor survival. However, in recent years an explosion of collaborative research into myeloma has identified molecular interactions between myeloma cells and the bone marrow microenvironm...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Herpes simplex: patterns of infection in patients attending a genitourinary medicine clinic, 2007-2012.
Authors: Daniels D, Mortlock S PMID: 24273898 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Examination of factors that influence residual chlorine concentration in chlorine-based sanitising solutions: implications for ward disinfection.
Authors: Moore JE, Thompson I, Buchanan J, Loughrey A PMID: 24273899 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Detecting HIV antibodies in oral fluid: validation of a commercial antigen-antibody assay.
Authors: Mortlock S, McLean F, Pickford C, Willis S PMID: 24273900 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Identification of Clostridium difficile: evaluation of genotypic, phenotypic and proteomic methods.
Authors: Jain A, Pope C, Wilks M, Planche T PMID: 24273901 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Reprocessing bloodstained ThinPrep Papanicolaou cervical cytology samples using glacial acetic acid increases the satisfactory rate in previously unsatisfactory smears.
Authors: Power Y, Bowes C PMID: 24273902 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Molecular cloning and characterisation of the methionine sulphoxide reductase A (msrA) gene locus in Campylobacter lari organisms.
Authors: Nakajima T, Matsubara K, Moore JE, Murayama T, Matsuda M Abstract The methionine sulphoxide reductase A (msrA) gene and its adjacent genetic loci from urease-negative (UN) Campylobacter lari RM2100 and urease-positive thermophilic Campylobacter (UPTC)CF89-12 strains appear to be composed of a msrA structure gene (507 base pairs [bp]) and another five-gene cluster (approximately 6300 bp) in the same strand and direction. A primer pair (F1/R4-msrA) for polymerase chain reaction (PCR) amplification was designed to generate a product of approximately 900 bp of the msrA gene, including its adjacent genetic loci...
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry: rapid identification of bacteria isolated from patients with cystic fibrosis.
This study shows that MALDI-TOF MS is superior to standard techniques in providing cheap, rapid and accurate identification of CF sputum isolates. PMID: 24400425 [PubMed - indexed for MEDLINE] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 2, 2015 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research