Development and evaluation of a novel score for prediction of large oesophageal varices in patients with hepatitis c virus-induced liver cirrhosis.
CONCLUSION: PAP scores a non-invasive, inexpensive and simple score that could predict the presence of large oesophageal varices reducing the need of endoscopy. The PAP score has a superior AUC score than other scores, suggesting improved clinical value. PMID: 28504002 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 17, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Fluorescent PCR detection of mecA in drug resistant MRSA: a methodological study.
Authors: Cao Y, Hao Y, Yang D, Pang B, Wang L PMID: 28493763 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 13, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Expression of WT1 and PAX8 in the epithelial tumours of Malaysian women with ovarian cancer.
Authors: Rhodes A, Vallikkannu N, Jayalakshmi P Abstract BACKGROUND: Ovarian cancer is particularly lethal due to late stage at presentation. The subtypes behave differently with respect to their biology and response to treatment. Two recent markers reported to be useful in assisting in the diagnosis are WT1 and PAX8. Malaysia, with its multi-ethnic population provides an opportunity to study the expression of these biomarkers in ovarian cancer in the three most populous ethnicities in Asia and ascertain their usefulness in the diagnosis of ovarian carcinoma. MATERIALS AND METHODS: Tissues from ovarian epitheli...
Source: British Journal of Biomedical Science - April 5, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Circulating levels of collagen III and MMP-1 in patients with chronic hepatitis C co-infected with hepatitis B virus.
CONCLUSION: HCV/HBV co-infected and HCV- mono-infected patients had similar clinical characteristics and there is no effect of HBV co-infection on the progression rates of liver fibrosis in chronic hepatitis C patients. PMID: 28367737 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 5, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Comparison of the fast track diagnostics respiratory 21 and Seegene Allplex multiplex polymerase chain reaction assays for the detection of respiratory viruses.
CONCLUSION: Although the performance of the assays were similar, the Seegene assay had the advantage of simultaneous detection of two gene targets for each of the common Influenza A subtypes, improved throughput of 30 samples per run and automated result analysis. The FTD assay could only test 17 samples per run but validation for use on several different real-time thermal cyclers made it easier to integrate into an existing laboratory system. Both assays were cost effective compared to in-house multiplex PCR respiratory virus screening. PMID: 28367738 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 5, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Towards understanding clinical campylobacter infection and its transmission: time for a different approach?
Authors: Casey E, Fitzgerald E, Lucey B Abstract Campylobacter spp. are among the most commonly diagnosed causes of human infection. Methods for detection of the 29 campylobacter species have mainly focused on cultivation of the thermophilic species. More than 99% of clinical campylobacter isolates notified in the UK in the recent past have been from faecal samples and associated with gastroenteritis. Campylobacter enteritis notifications in temperate zones show a seasonal increase during the summer months with a sharp decrease in the winter months, a pattern which remains incompletely understood. The striking seas...
Source: British Journal of Biomedical Science - April 5, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

miR-199a-3p downregulation in thyroid tissues is associated with invasion and metastasis of papillary thyroid carcinoma.
CONCLUSIONS: Our data suggest that downregulation of miR-199a-3p in thyroid tissues is linked to invasion and metastasis of PTC and may be a potential target for therapeutic intervention. PMID: 28367740 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - April 5, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Integron occurrence is linked to reduced biocide susceptibility in multidrug resistant Pseudomonas aeruginosa.
CONCLUSIONS: The current study reveals the presence of different classes of integrons, with class I being predominant. Class I integron may be responsible for generating MDR P. aeruginosa isolates with reduced susceptibility to biocides. This linkage between integrons and biocide resistance in MDR-Pseudomonas isolates is notable and could be clinically important. Strict antibiotic prescription policies and the adequate use of biocides could help in controlling this problem. PMID: 28281934 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - March 12, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

British Journal of Biomedical Science in 2016. What have we learned?
Authors: Blann A Abstract In 2016, the British Journal of Biomedical Science published 36 reports outlining specific advances in each of the various disciplines within biomedical science. These were one review, 25 original articles, 9 'In Brief' reports and one letter to the Editor. Of these, the majority were in blood science (5 in biochemistry, 7 in haematology and 2 in immunology) and infection science (8 in microbiology, 2 in virology) with a smaller number in cellular sciences (6 in cellular pathology and 2 in cytopathology). Three reports considered both biochemistry and immunology, while another reported an ...
Source: British Journal of Biomedical Science - February 17, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Diagnostic virology and patient care: from vaguely interesting to vitally important.
Authors: Pitt SJ, Phillips DI Abstract The existence of pathogenic viruses was inferred by experiments at the turn of the twentieth century. Key developments in detection of viruses, including electron microscopy and monolayer cell culture, were made in the middle of that century. However, in terms of patient care, the results from the virology laboratory often arrived the patient was 'better or dead'. The advent of molecular techniques, particularly polymerase chain reaction and more recently whole genome sequencing made timely and accurate diagnosis of viral infections feasible. A range of approaches have been ta...
Source: British Journal of Biomedical Science - February 17, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The relationship between Multidrug Resistance Protein 1(rs1045642) and Cholesterol 24-hydroxylase (rs754203) genes polymorphism with type 2 diabetes mellitus.
Authors: Rizvi S, Raza ST, Mehdi SR, Siddiqi Z, Eba A, Mahdi F Abstract BACKGROUND: The involvement of genetic factors like gene polymorphisms has been found to contribute significantly to the development and progression of type 2 diabetes (T2DM). Thousands of single nucleotide polymorphisms in various genes have been found to be associated with risk of T2DM. The present study was aimed to investigate association of Multidrug resistance 1 (MDR1) (rs1045642) and CYP46A1 (rs754203) genes polymorphism with T2DM. SUBJECTS & METHODS: Study includes 333 subjects, 183 T2DM cases and 150 healthy controls. Single nu...
Source: British Journal of Biomedical Science - February 17, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Fasting urinary calcium to creatinine ratio for the evaluation of calcium nephrolithiasis in adults.
Authors: Sun L, Guo Z, Shan J, Jiang G PMID: 28102104 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - January 21, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Potential route of Th17/Treg cell dynamics in targeting type 1 diabetes and rheumatoid arthritis: an autoimmune disorder perspective.
Authors: Karri SK, Sheela A Abstract Cytokines, small secreted proteins, have a specific effect on the interactions and communications between cells. They play a pivotal role in the pathogenesis of autoimmune diseases. Factors in the breakdown of self-tolerance and the subsequent events leading to the induction of pathogenic responses remain unclear for most of the autoimmune diseases. Large numbers of studies have revealed a general scheme in which pro-inflammatory cytokines contribute to the initiation and propagation of autoimmune inflammation, whereas anti-inflammatory cytokines facilitate the regression of inf...
Source: British Journal of Biomedical Science - January 12, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Assessment of semi-automated nucleic acid testing programme in a Regional Blood Transfusion Centre.
CONCLUSION: The semi-automated combined system for NAT screening assays is robust, sensitive, reproducible, and this gives an additional layer of safety with affordable cost. PMID: 27996693 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 22, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A breath ammonia analyser for monitoring patients with end-stage renal disease on haemodialysis.
Authors: Limeres J, Garcez JF, Marinho JS, Loureiro A, Diniz M, Diz P Abstract OBJECTIVE: Breath ammonia measurement has attracted increasing interest for clinical diagnosis and metabolic status monitoring. This pilot study aims to evaluate a simple detection method to measure breath ammonia levels in haemodialysis patients. MATERIALS AND METHODS: The study group comprised 44 adults undergoing haemodialysis and a control group of 44 age- and sex-matched individuals with a glomerular filtration rate>90 mL/min. To measure breath ammonia concentration, we designed a device based on that used to monitor atm...
Source: British Journal of Biomedical Science - December 16, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Multiplex PCR for detection of Helicobacter pylori infection in gastric biopsies with lower inflammatory score.
CONCLUSION: PCR was able to detect the highest numbers of positive cases although the lowest average scores were recorded in the activity, inflammatory and H. pylori density. PMID: 27922429 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 8, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Downregulation of miR-124 predicts poor prognosis in pancreatic ductal adenocarcinoma patients.
Authors: Sun B, Liu X, Gao Y, Li L, Dong Z Abstract BACKGROUND AND AIMS: miRNA-124 (miR-124) expression was known to be downregulated in patients with pancreatic ductal adenocarcinoma (PDAC). Downregulation of miR-124 was significantly associated with poor prognosis in patients with PDAC. Recent studies have shown that circulating miRNAs could be the potential biomarkers for invasive diagnostic as well as prognostic purposes. The purpose of the current study was to characterise the serum miR-124 levels and assess the clinical significance of serum miR-124 in patients with PDAC. METHODS: Using quantitative rever...
Source: British Journal of Biomedical Science - December 8, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A multicentre study of the precision and accuracy of the TruSlice and TruSlice Digital histological dissection devices.
Authors: Orchard GE, Shams M, Nwokie T, Fernando P, Bulut C, Quaye CJ, Gabriel J, Ramji Z, Georgaki A, Watt M, Cole Z, Stewart K, McTaggart V, Padayachy S, Long AM, Ogden A, Andrews C, Birchall A, Shams F, Neesam H, Haine N Abstract BACKGROUND: Five key factors enabling a good surgical grossing technique include a flat uniformly perpendicular specimen cutting face, appropriate immobilisation of the tissue specimen during grossing, good visualisation of the cutting tissue face, sharp cutting knives and the grossing knife action. TruSlice and TruSlice Digital are new innovative tools based on a guillotine configurati...
Source: British Journal of Biomedical Science - December 8, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

miR-21 predicts poor prognosis in patients with osteosarcoma.
Authors: Ren X, Shen Y, Zheng S, Liu J, Jiang X Abstract BACKGROUND AND AIMS: miR-21 has been demonstrated to play an important role in tumour progression. The aim of the present study was to analyse the correlation between miR-21 expression level and clinicopathologic features, as well as to assess the prognostic significance of miR-21 in osteosarcoma. METHODS: Eighty-four pairs of osteosarcoma and corresponding non-cancerous bone tissues were obtained, and miR-21 expression levels were detected using quantitative real-time PCR (qRT-PCR). A χ(2) test was used to assess the relationship betwe...
Source: British Journal of Biomedical Science - December 8, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

MicroRNAs: the future of genomic science?
Authors: PMID: 27922433 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - December 8, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

miR-146a gene polymorphism and susceptibility to gastric cancer.
Authors: Yadegari ZS, Akrami H, Hosseini SV, Erfani N PMID: 27762791 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - October 21, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Platelet-rich plasma for tissue regeneration can be stored at room temperature for at least five days.
CONCLUSION: Although platelet integrity and PDGF-BB fell over time, the intermittently agitated non-oxygen permeable container appeared to retain better platelet integrity and function, and PDGF-BB release, than other storage conditions, with potential for clinical use for 5-8 days. PMID: 27718888 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - October 13, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

ABCB1 polymorphisms and steroid treatment in children with idiopathic nephrotic syndrome.
CONCLUSION: Our results suggested that C1236T polymorphism in ABCB1 gene was associated with steroid resistance. A higher proportion of SR children had C1236T TT genotype and T allele, these patients may require other therapeutic strategies. PMID: 27719329 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - October 13, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Neutrophil to lymphocyte ratio and platelet to lymphocyte ratio as diagnostic markers for pneumonia severity.
Authors: Lee JH, Song S, Yoon SY, Lim CS, Song JW, Kim HS PMID: 27723441 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - October 13, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum CX3CL1/fractalkine concentrations are positively associated with disease severity in postmenopausal osteoporotic patients.
This study aimed to investigate the link between serum CX3CL1/FKN levels with disease progression of postmenopausal osteoporotic patients. METHODS: A total of 53 women with postmenopausal osteoporosis (PMOP group), 51 postmenopausal non-osteoporotic female patients (PMNOP group) and 50 premenopausal non-osteoporotic healthy women of childbearing age (control group) were enrolled in the study. The bone mineral density (BMD) for all subjects was determined via dual-energy X-ray absorptiometry of the lumbar spine, femoral neck, internal trochanter, total hip, greater trochanter and Ward's triangle. The levels of FKN in th...
Source: British Journal of Biomedical Science - August 3, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Immunophenotyping of T cells in the peripheral circulation in psoriasis.
CONCLUSION: Our results suggest an immune-dysregulation in psoriasis associated with a predominance of Th1/Th17 phenotype, especially with increasing severity of the disease. PMID: 27477596 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - August 3, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Seasonal variation of whole blood copper levels in children aged 0-14  years.
Seasonal variation of whole blood copper levels in children aged 0-14 years. Br J Biomed Sci. 2016 Aug 1;:1-4 Authors: Zhang M, Zhai R, Liu J, Guang H, Li B, Chen D Abstract BACKGROUND: Both Cu deficiency and excess, high and low Cu levels even within normal range are detrimental to human health. Therefore, our study aims to evaluate seasonal variation in whole blood copper (Cu) levels in children aged 0-14 years. METHODS: Whole blood samples were obtained from 3210 children who came for a routine health examination. Cu levels were determined by flame atomic absorption spectrometry. RESULT...
Source: British Journal of Biomedical Science - August 3, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Hypophosphataemia and parenteral nutrition; biochemical monitoring, incidence and outcomes.
Authors: Fung EC, Wickramasinghe SR, Panteli JV, Crook MA Abstract AIMS: The aim of this study was to assess the incidence and clinical associations of hypophosphataemia in patients undergoing parenteral nutrition (PN) and the importance of biochemical monitoring. METHODS: Patients (n = 57) on PN were identified from pharmacy and also dietician referral records. Access to patient case files and dietetics records provided details pertaining to diagnosis, indications for PN, the level of biochemistry monitoring, and any complications incurred. Hypophosphataemia
Source: British Journal of Biomedical Science - August 3, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation of some haemostatic parameters in falciparum malaria and HIV co-infection.
CONCLUSION: These results suggest that co-infected subjects with severe malaria have a higher risk of bleeding and would require greater monitoring. PMID: 27447335 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 26, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Laboratory monitoring of rivaroxaban and assessment of its bleeding risk.
CONCLUSIONS: Biophen DiXaI can be considered as a quantitative method to monitor the anticoagulation activity of rivaroxaban, and could be used to evaluate bleeding risk caused by rivaroxaban. The PT reagent (Thrombosis S) could be considered as a rough method to monitor the anticoagulation activity of rivaroxaban and evaluate bleeding risk caused by rivaroxaban. PMID: 27400196 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 13, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The immunomodulatory activity of secondary metabolites isolated from Streptomyces calvus on human peripheral blood mononuclear cells.
In this study, the immunomodulatory effects of S. calvus secondary metabolites on the expression of various cytokine genes by human peripheral blood mononuclear cells (PBMCs) were evaluated. METHODS: A bacterial sample was inoculated in Mueller Hinton Broth and secondary metabolites were extracted. PBMCs were isolated from venous blood and were treated with S. calvus secondary metabolites for 48 h. The cell proliferation was assessed by Methyl tetrazolium bromide (MTT) assay and quantitative real-time polymerase chain reaction (qRT-PCR) assays to survey mRNA expressions of selected pro-inflammatory and inhibitory ...
Source: British Journal of Biomedical Science - July 7, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Negative regulation of TIM-3 expression in AML cell line (HL-60) using miR-330-5p.
CONCLUSION: According to our results, miR-330-5p has a strong inhibitory effect on TIM-3 expression in AML cell line. Thus, the bioinformatics prediction of Mirwalk and Target Scan softwares for silencing effect of miR-330-5p on TIM-3 is confirmed. PMID: 27341144 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 26, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Immunostimulation by cytosine-phosphate-guanine oligodeoxynucleotides in combination with IL-2 can improve the success rate of karyotype analysis in chronic lymphocytic leukaemia.
CONCLUSION: CpG-ODN DSP30 combined with IL-2 is effective in improving the detection rate of chromosomal abnormalities in CLL cells. This combination with FISH analysis is conducive to increasing the detection rate of genetic abnormalities in CLL. PMID: 27327088 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 23, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Comparison of two new generation influenza rapid diagnostic tests with instrument-based digital readout systems for influenza virus detection.
CONCLUSIONS: Digital-based readout systems for the detection of the influenza virus can be applied for more sensitive diagnosis in clinical settings than conventional RDTs. PMID: 27327199 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 23, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Automated measurement of 25-OH Vitamin D on the LUMIPULSE(®) G1200: analytical verification and method comparison.
Automated measurement of 25-OH Vitamin D on the LUMIPULSE(®) G1200: analytical verification and method comparison. Br J Biomed Sci. 2016 Jun 16;:1-6 Authors: Parra M, Foj L, Filella X Abstract BACKGROUND: Because of its potential value in several pathologies, clinical interest in 25-hydroxy Vitamin D (25OH-D) is increasing. However, the standardisation of assays remains a significant problem. Our aim was to evaluate the performance of the novel Lumipulse G 25-OH Vitamin D assay (Fujirebio), comparing results with the Liaison (Diasorin) method. METHODS: Analytical verification of the Lumipulse G...
Source: British Journal of Biomedical Science - June 17, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Endothelial progenitor cells mobilisation after percutaneous coronary intervention: a pilot study.
CONCLUSIONS: Baseline EPC levels are related with the extension of the lesion and stenosis whereas the kinetics of EPC mobilization showed to be related with C-reactive protein concentration. Endothelial activation seems to occur in response to EPC mobilization or vascular damage by PCI. PMID: 27310267 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 17, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Proteomic profile of cerebrospinal fluid in patients with multiple sclerosis using two dimensional gel electrophoresis.
CONCLUSION: The results of this study support the presence of changes in the CSF proteome of MS patients. There is a need to investigate the role of these candidate CSF spots in a larger number of MS patients. PMID: 27254308 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 4, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum bilirubin: a simple routine surrogate marker of the progression of chronic kidney disease.
Authors: Moolchandani K, Priyadarssini M, Rajappa M, Parameswaran S, Revathy G Abstract BACKGROUND: Studies suggest that Chronic Kidney Disease (CKD) is a global burden health associated with significant comorbid conditions. Few biochemical parameters have gained significance in predicting the disease progression. The present work aimed to study the association of the simple biochemical parameter of serum bilirubin level with the estimated glomerular filtration rate (eGFR), and to assess their association with the co-morbid conditions in CKD. METHODS: We recruited 188 patients with CKD who attended a Nephrology...
Source: British Journal of Biomedical Science - May 29, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Calcitriol prevents inflammatory gene expression in macrovascular endothelial cells.
Authors: Gonzalez-Curiel I, Marin-Luevano P, Trujillo V, Enciso-Moreno JA, Gonzalez-Castillo C, Rivas-Santiago B Abstract BACKGROUND: Calcitriol (vitamin D) supplementation has been proposed for therapeutical use in vascular diseases due to its immunomodulatory activity, preventing inflammation and promoting angiogenesis. In the present study, we hypothesised whether calcitriol downregulates pro-inflammatory gene expression without affecting angiogenesis and anti-inflammatory gene expression in LPS-induced endothelial cells. METHOD: In order to evaluate the effect of calcitriol in suppressing inflammatory gene ...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Preliminary feasibility and modelling of a liquid matrix Dictyostelium discoideum virulence assay for Pseudomonas aeruginosa.
Authors: Champion AC, Houston NK, Bradbury RS, Reid DW Abstract OBJECTIVES: To develop and determine the feasibility of using a liquid matrix adaptation of the Dictyostelium discoideum bacterial virulence assay by testing on well-characterised clinical and environmental isolates of Pseudomonas aeruginosa. MATERIALS AND METHODS: Axenic AX2 D. discoideum were co-cultured with clinical and environmental isolates of P. aeruginosa in costar 24-well tissue culture plates for 24 h. A P. aeruginosa PAO1 positive control was tested in biological quintuplicate. Wells were then inspected using an inverted microscope ...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Serum TGF-β1 in patients with acute myocardial infarction.
Serum TGF-β1 in patients with acute myocardial infarction. Br J Biomed Sci. 2016 Apr 28;:1-4 Authors: Liu Z, Zhang K, Wang Q, Li Y, Zhang L PMID: 27181170 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Thyroid and parathyroid hormones in benign prostatic hyperplasia.
Authors: Eldhose A, Nandeesha H, Dorairajan LN, Sreenivasulu K, Arul Vijaya Vani S PMID: 27181171 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Molecular identification and characterisation of catalase and catalase-like protein genes in urease-positive thermophilic Campylobacter (UPTC).
CONCLUSIONS: This study demonstrates the presence of catalase/catalase-like protein genes in UPTC organisms. These findings are significant in that they suggest that UPTC organisms have the protective genetic capability of helping protect the organisms from toxic oxygen stress, which may help them to survive in physiologically harsh environments, both within human and animal hosts, as well as in the natural environment. PMID: 27181172 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Identification of minor chromosomal defects causing abnormal foetus and spontaneous abortions.
CONCLUSION: Therefore, we conclude that use of a high-density SNP platform in diagnosis can give better understanding of molecular causes of pregnancy loss and foetal abnormalities. PMID: 27181173 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Antimicrobial resistance in cystic fibrosis isolates of Haemophilus influenzae.
Authors: Atkinson CT, Tristram SG PMID: 27181174 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Aseptic meningitis after measles-mumps-rubella (MMR) vaccination.
Authors: Mamishi S, Sarkardeh M, Pourakbari B, Keshavarz Valian S, Sabouni F, Mahmoudi S PMID: 27181175 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Cervical lymph node thyroglobulin measurement in washout of fine-needle aspirates for diagnosis of papillary thyroid cancer metastases.
Authors: Li J, Zhang K, Liu X, Hao F, Liu Z, Wang Z Abstract BACKGROUND: Patients with papillary thyroid cancer (PTC) and enlarged cervical lymph nodes (CLNs) are usually assessed by fine-needle aspiration biopsy cytology (FNAB-C). Thyroglobulin (Tg) is frequently detected in washout of fine-needle aspirates (FNA) of these lymph nodes. The aim of this study was to evaluate the accuracy of the measurement of FNAB-Tg in the washout of FNAB in combination with FNAB-C to detect CLN metastases in PTC. METHODS: We retrospectively evaluated 163 surgically proven CLNs. Ultrasound-guided FNAB-C and FNAB-Tg measurements ...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The rolling evolution of biomedical science as an essential tool in modern clinical practice.
Authors: Blann A Abstract The British Journal of Biomedical Science is committed to publishing high-quality original research that represents a clear advance in the practice of biomedical science, and reviews that summarise recent advances in the field of biomedical science. The overall aim of the Journal is to provide a platform for the dissemination of new and innovative information on the diagnosis and management of disease that is valuable to the practicing laboratory scientist. The Editorial that follows describes the Journal and provides a perspective of its aims and objectives. PMID: 27182669 [PubM...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

British Journal of Biomedical Science in 2015: what have we learned?
Authors: Blann A, Nation B Abstract In 2015, the British Journal of Biomedical Science published 47 reports on topics relating to the various disciplines within biomedical science. Of these, the majority were in infection science (15 in microbiology and two in virology) and blood science (seven in biochemistry, four in haematology, three in immunology and one in transplantation), with a smaller number in cellular sciences (four reports) and with one review across disciplines. The present report will summarise key aspects of these publications that are of greatest relevance to laboratory scientists. PMID: 27182...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Platelet function testing: from routine to specialist testing.
Authors: Gurney D Abstract Platelets have many functions within the haemostatic system, and when these actions are diminished for whatever reason, a bleeding tendency can manifest. Unravelling the reason(s) for this bleeding can be complex due to the multiple roles platelets perform. This review seeks to explain each level of platelet testing moving from those performed at local hospital laboratories to those performed by specialist centres and university research departments. It will examine the testing available and discuss when to move on to additional testing. PMID: 27182671 [PubMed - in process] (Source: ...
Source: British Journal of Biomedical Science - May 20, 2016 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research