Accelerated detection of extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae.
Accelerated detection of extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae. Br J Biomed Sci. 2006 Jan;63(4):151-154 Authors: Kader AA, Kamath KA, Dass SM Abstract A prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Escherichia coli, Klebsiella pneumoniae and other Gram-negative bacteria. A modified double-disc test (MDDT) is incorporated in a Gram-negative template for routine susceptibility testing. The MDDT identified accurately ESBLs in all isolat...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Detection of Dientamoeba fragilis by culture.
Authors: Windsor JJ, Macfarlane L, Hughes-Thapa G, Jones SKA, Whiteside TM Abstract Symptoms associated with Dientamoeba fragilis include diarrhoea, abdominal pain, nausea, vomiting, epigastric pain and weight loss. A possible link between D. fragilis and irritable bowel syndrome (IBS)-like symptoms has been reported, and therefore the presence of this parasite should be excluded before making a diagnosis of IBS. Over a six-month period, 976 faecal samples were submitted to NPHS Microbiology Aberystwyth for routine microbiological analysis. All samples were also cultured for parasites using Robinson's xenic medium....
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Genetics of the lipoprotein lipase gene and hypertriglyceridaemia.
Authors: Mcdonnell MG, Young IS, Nicholls DP, Archbold GPR, Graham CA Abstract The aim of this study is to assess whether genetic variation at the lipoprotein lipase (LPL) gene is related to fasting triglyceride levels or to the presence of vascular disease. Hypertriglyceridaemic patients are genotyped for the N291S, G188E, and P207L variants and the HindIII and PvuII restriction fragment length polymorphisms of the LPL gene. Sequence analysis is carried out on exons 1-9 of the LPL gene for patients with severe hypertriglyceridaemia, to search for new gene variants. No differences were found between the patient and...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Screening for methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci: an evaluation of three selective media and Mastalex-MRSA latex agglutination.
Authors: Bowers KM, Wren MWD, Shetty NP Abstract Laboratory confirmation of MRSA is important for the implementation of infection control; conventional screening culture methods take up to five days for confirmation. The purpose of this study is to ascertain the efficiency of three selective media for growth of methicillin-resistant Staphylococcus aureus (MRSA) before and after enrichment in salt broth, and to evaluate the Mastalex-MRSA latex agglutination kit for detection of methicillin resistance. Screening swabs were collected from 63 patients, yielding125 S. aureus isolates and 40 coagulase-negative staphyloco...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Strategies for detecting toxoplasma immunity.
Authors: Ashburn D, Evans R, Chatterton JMW, Joss AWL, Ho-Yen DO Abstract A strategy for identifying toxoplasma immunity in pregnancy must provide good evidence of immunity but not falsely reassure; that for immunocompromised patients should identify immunity and also the risk of reactivated toxoplasmosis. Using sera from both of these patient groups, the performance of an in-house IgG EIA and two commonly used commercial assays (Abbott AxSYM Toxo-G and Eiken latex test) were compared with the dye test. False-positive results were obtained using the IgG enzyme immunoassay (EIA) and AxSYM Toxo-G, and false negatives...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Preventive and protective effects of wild basil in ethanol-induced liver toxicity in rats.
Authors: Chaturvedi P, George S, John A Abstract In the present study, preventive and protective effects of Ocimum gratissimum in ethanol-induced hepatotoxicity are assessed in albino rats. A methanol extract of O. gratissimum leaves is prepared, with a yield of 3.5% (w/w) of the dry weight of leaves. Graded doses of the extract (10, 20, 40 and 80 mg/kg body weight), together with ethanol (5 gm/kg body weight) are administered orally to experimental groups for 30 days. Normal control rats receive distilled water only, while rats in an alcohol control group (AC) receive ethanol only for 30 days. O. gratissimum reduc...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Quantitative analysis of human leucocyte antigen expression during culture of Epstein-Barr virustransformed cell lines using the Dako QIFIKIT.
Authors: Battle R, Clark B Abstract Pre-existing donor-specific human leucocyte antigen (HLA) antibodies in renal allograft recipients result in hyperacute and accelerated graft failure. These antibodies can be detected in flow cytometric assay systems using HLA-characterised Epstein-Barr virus (EBV)-transformed B-lymphocyte cell lines. Confident assay performance is predicated by the expression of HLAs on the EBV-transformed B-cell line surface. Surface HLA expression of three EBV-transformed B-cell lines that had previously been used as part of a potential organ recipient serum screening panel at St James' Univer...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Lack of horizontal gene transfer of methicillin-resistance genetic determinants from PBP2a-positive, coagulase-negative staphylococci to methicillin-sensitive Staphylococcus aureus using transcutaneous electrical nerve stimulation (TENS).
Authors: Wishart K, Loughrey A, Mcclurg RB, Goldsmith CE, Millar BC, Rao J, Sengupta B, Dooley JSG, Rooney PJ, Moore JE Abstract Previous research shows that approximately half of the coagulase-negative staphylococci (CNS) isolated from patients in the intensive care unit (ICU) at Belfast City Hospital were resistant to methicillin. The presence of this relatively high proportion of methicillin-resistance genetic material gives rise to speculation that these organisms may act as potential reservoirs of methicillin-resistance genetic material to methicillin-sensitive Staphylococcus aureus (MSSA). Mechanisms of horiz...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Cook DJ PMID: 28700891 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Combined use of nuclear phosphoprotein c-Myc and cellular phosphoprotein p53 for hepatocellular carcinoma detection in high-risk chronic hepatitis C patients.
Authors: Attallah AM, El-Far M, Abdelrazek MA, Omran MM, Attallah AA, Elkhouly AA, Elkenawy HM, Farid K Abstract BACKGROUND: Hepatocellular carcinoma (HCC) is a multistage process resulting from various genetic changes. We aimed to determine nuclear phosphoprotein c-Myc and cellular phosphoprotein p53 expression and to evaluate their importance in HCC diagnosis. METHODS: One hundred and twenty chronic hepatitis C (CHC) patients (60 non-HCC CHC patients and 60 HCC patients who had a single small (
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Anaplastic lymphoma kinase (ALK) mutations in patients with adenocarcinoma of the lung.
Authors: Mohamad N, Jayalakshmi P, Rhodes A, Liam CK, Tan JL, Yousoof S, Rajadurai P Abstract BACKGROUND: Non-small cell lung cancer (NSCLC) is a major cause of cancer-related death. Approximately 2-16% of NSCLC patients with wild-type epidermal growth factor receptor (EGFR) harbour anaplastic lymphoma kinase (ALK) mutations. Both EGFR and ALK mutations occur most commonly in Asian patients with NSCLC. As targeted therapy is available for NSCLC patients with these mutations, it is important to establish reliable assays and testing strategies to identify those most likely to benefit from this therapy. MATERIALS ...
Source: British Journal of Biomedical Science - July 15, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation of the mastascanelite image analysis system for measuring zones of inhibition in disc diffusion susceptibility tests.
Authors: Clarke REH, Winstanley TG, Ridgway EJ Abstract In this evaluation a mastascanelite image analysis system is compared with manual measurement of disc diffusion inhibition zones. Data for 213 diverse organisms and a total of 1679 organism/antibiotic combinations gave an overall correlation coefficient of 0.988. The mean difference between readings was +0.425 mm, with 95% confidence limits of ±2.94 mm, and the majority of scanned zones (97.51%) fell within ±3 mm of the manual measurement. The mastascanelite system forms part of a laboratory suite and was found to be objective, accurate and rapid...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

CD54 and CD62L expression by lymphoid cells in acute lymphoblastic leukaemia in children.
Authors: Hafez FM, Hassab H, Morad Z, Farag EAM Abstract Altered expression or function of adhesion molecules on leukaemic blasts may contribute to the evolution and biological behaviour of acute leukaemia. This work studies the expression of CD54 and CD62L by lymphoid cells and the serum level of the shed form of L-selectin (sL-selectin) in children with acute lymphoblastic leukaemia (ALL) at initial diagnosis and after first remission, and their relationship to disease activity and subtype. The study is conducted on 20 children (age range 2-10 years) newly diagnosed with ALL and admitted to Alexandria University ...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Determination of verocytotoxin and eae gene loci by multiplex PCR in Escherichia coli O157:H7 isolated from human faeces in Northern Ireland: a four-year study of trends, 1997-2000.
This study aims to determine the distribution and frequency of verocytotoxin genes in human faecal clinical isolates of Escherichia coli O157 in Northern Ireland during the period 1997-2000, using a special four-target multiplex polymerase chain reaction (PCR) assay. One hundred and thirty two isolates of E. coli O157:H7 cultured during the four-year period (1997 [n=28]; 1998 [n=25]); 1999 (n=43); 2000 [n=36]), representing approximately 79% of total E. coli O157 laboratory isolations throughout N. Ireland, are examined for the presence of verocytotoxin gene loci (VT1, VT2 and eae) using a multiplex PCR assay. These isolat...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Leucodepletion for transmissible spongiform encephalopathies.
Authors: Romaine CS, Hazlehurst G, Jewell AP Abstract Transmissible spongiform encephalopathies (TSEs) have been recognised around the world for many years. Creutzfeldt-Jakob disease (CJD), one of the human forms of TSE, has been studied widely and thus far has not proved a great threat to human health. The emergence of two new TSEs - bovine spongiform encephalopathy (BSE) in cattle and variant Creutzfeldt-Jakob disease (vCJD) in humans in the UK - has caused great concern. BSE has had an economic impact and vCJD is a threat to human health. It has been shown that these two diseases are caused by the same prion age...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Histopathology Specimens: Clinical, Pathological and Laboratory Aspects.
Authors: Orchard GE PMID: 28696866 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Biomedical Science in Brief.
Authors: Stanley DH, Wareing DRA, Murphy PG, Millar BC, Wilson T, Moore JE, Mcchlery SM, Kerrigan J, Clarke SC, Mortlock DH, Gibbs G, Bolam S, Adeiga A, Akinosho RO, Audu RA, Onyuewuche J, Oyewole O PMID: 28696867 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Seroprevalence of antibodies to conserved regions of Chlamydia trachomatis heat shock proteins60 and 10 in women in India.
In this study, 255 patients attending the gynaecology out-patient department (March 2004 to August 2005) of Safdarjung Hospital were enrolled. Of these patients, 107 were diagnosed with cervicitis while 52 had pelvic inflammatory disease (PID)/infertility. Chlamydia trachomatis infection in endocervical specimens is diagnosed by a direct fluorescence assay (DFA) and the polymerase chain reaction (PCR). In 75 (29.4%) of the C. trachomatis-positive women, 50 (66.7%) were ELISA positive for MOMP, 48 (64.0%) were positive for cHSP60 and 46 (61.3%) were positive for cHSP10. The anti-MOMP index correlated positively with anti-cHSP60 (R=0.522, P
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Overfield J, Webb SJ, Wilkinson M, Edirisinghe S PMID: 28696886 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Biomedical Science in Brief.
Authors: Millar BC, Xu J, Earle JAP, Evans J, Moore JE, Orchard GE, Guthrie JS, Mckenzie H, Ho-Yen DO, Hajeer AH, Knawy A, Alhaj-Hussein BT, Al-Rubiaan SD PMID: 28696887 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A six-month audit of the isolation of Fusobacterium necrophorum from patients with sore throat in a district general hospital.
This study was performed over a six-month period (October 2004 to March 2005) at the Eastbourne District General Hospital. All throat swabs received in the laboratory are cultured routinely for haemolytic group A streptococci and pathogenic Corynebacteria spp. During the study period an extra fastidious anaerobic blood agar plate with neomycin was inoculated, with a 30 µg vancomycin disc placed at the junction of the second and third streaks. This was examined after 48 h for the presence of F. necrophorum. A total of 1157 swabs were processed during the study period: 156 were positive for haemolytic group A streptoco...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Biomedical Science in Brief.
Authors: Banks M, Wilson G, Peel DA, Mabayoje VO, Oparinde DP, Akanni EO, Taiwo SS, Muhibi MA, Adebayo TO PMID: 28696889 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Modulation of the antioxidant defence in different developmental stages of Schistosoma mansoni by praziquantel and artemether.
Authors: El-Bassiouni EA, Helmy MH, Saad EI, Kamel EN, Abdel-Meguid E, Hussein HSE Abstract Human schistosomiasis is a chronic and debilitating parasitic disease caused by parasitic trematode worms (schistosomes). Praziquantel (PZQ) is the drug of choice as it is active against all Schistosoma species, can be administered easily, has high cure and egg reduction rates, with no or only mild side effects. Rapid re-infection following treatment and the concerns about PZQ resistance has led to the search for new drugs to treat schistosomiasis. Significant progress has been made with artemisinin derivatives (e.g., arteme...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Hb Q(India) and its interaction with β-thalassaemia: a study of 64 cases from India.
Hb Q(India) and its interaction with β-thalassaemia: a study of 64 cases from India. Br J Biomed Sci. 2007 Jan;64(4):160-163 Authors: Phanasgaonkar S, Colah R, Ghosh K, Mohanty D, Gupte S Abstract Haemoglobin Q (Hb Q), a relatively uncommon α-chain structural Hb variant, has been reported either in the heterozygous state or interacting with β-thalassaemia. Individuals inheriting Hb Q generally are asymptomatic and are diagnosed by chance during population screening or as a part of a family study. This paper represents the first large study from India of 64 cases of Hb Q, documenting the...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Antimicrobial activity of extracts of the lichens Cladonia furcata, Parmelia caperata, Parmelia pertusa, Hypogymnia physodes and Umbilicaria polyphylla.
Authors: Ranković B, Mišić M, Sukdolak S Abstract The antimicrobial activity of acetone, methanol and aqueous extracts of the lichens Cladonia furcata, Parmelia caperata, Parmelia pertusa, Hypogymnia physodes and Umbilicaria polyphylla is assessed. The extracts are tested on six species of bacteria and 10 species of fungi using the disk-diffusion method, and broth tube dilution is used to determine minimal inhibitory concentration (MIC). The tested bacteria were more sensitive than the tested fungi. Aqueous extracts of the investigated lichens showed no antimicrobial activity against any of the test organi...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Book Reviews.
Authors: Dawson M, Hardy S, Blann AD PMID: 28696893 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Use of bioinformatics and PCR in the search for ABC transporter homology among various bacteria.
Authors: Jauhangeer BR, Wren MWD, Macdonald RAC, Perry D, Greenwell P Abstract Bioinformatics databases and search tools are utilised to produce polymerase chain reaction (PCR) primers for the amplification of an ABC transporter gene from the clinically important anaerobe Finegoldia magna. On sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of Enterococcus faecalis. Little sequence data is available for F. magna and the newly isolated DNA could be a useful tool in the identification of this organism in clinical specimens. PMID: 28696898 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Prions. A challenge to Science, Medicine and the Public Health System.
Authors: Somerville RA PMID: 28696899 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Aetiological agents of vaginitis in Nigerian women.
This study focuses on the identification of aetiological agents of vaginitis in Nigerian women. Study subjects are drawn from patients presenting with lower abdominal pain, vaginal discharge and itching at the gynaecology clinic of Lagos University Teaching Hospital and at the Clinical Centre of the Nigerian Institute of Medical Research, Yaba, Lagos, between January 2001 and July 2002. A total of 250 patients gave informed consent to participate in the study. The patients also had pre- and post-test human immunodeficiency virus (HIV) counselling. Each patient completed a questionnaire in order to provide biographical data...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Impact of new AABB guidelines on hepatitis B and C testing among Saudi blood donors.
This study aims to compare the advantages and disadvantages of implementing the new American Association of Blood Banks (AABB) guidelines for hepatitis B and C against its old criteria for screening blood donors. Between July 1995 and December 2002, 63,368 consecutive blood donors were screening for hepatitis B and C according to the new guidelines. Cost and prevalence were analysed and compared with those found using the old AABB guidelines prior to July 1995. The overall percentage rate of deferred donors showed a significantly decrease to 19.3% in 2002, compared to 58.4% before July 1995 (P
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation of the neutralising capacity of Bactec medium for some antibiotics.
In this study, the ability of the medium to neutralise the effect of some antibiotics and to destroy leucocytes is investigated. Vancomycin, amoxicillin, chloramphenicol, penicillin, gentamicin, fungizone and amikacin at various concentrations were added to separate bottles of Bactec medium 9240. Escherichia coli (NCTC 10418), Pseudomonas aeruginosa (NCTC 10662), Staphylococcus aureus (NCTC 6571) and Candida albicans (ATCC 10231), each at 1x10(4) colony-forming units (CFU)/mL were added separately, depending on the type of antibiotic. The blood samples were incubated at 37˚C for seven days under manual and automated blood...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Human chronic fascioliasis: a possible cause of unexplained abnormal liver tests.
In this study, the frequency of hepatitis B (HBV) and/or hepatitis C (HCV) in cases of chronic human fascioliasis is studied. Egg count, indirect haemagglutination test (IHAT), haemoglobin level, total leucocyte and eosinophil counts, serum bilirubin, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and acid phosphatase (ACP) are performed. Serum γ-glutamyltransferase (GGT), arylsulphatase (ASA) and lipid peroxide levels are determined. Results showed that levels of the latter group of enzymes were increased significantly in cases of chronic fascioliasis. Therefore, d...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Induction of apoptosis in myeloid progenitors by granulocyte-macrophage colony-stimulating factor.
Authors: Alenzi FQB Abstract Spontaneous apoptosis of normal purified bone marrow CD34(+) cells induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) via the Fas pathway appears to be mediated by caspase-1 and caspase-8 activity. In seeking an alternative explanation for this observation, the present study examined CD34(+) cell growth with different cytokines, cytokine concentrations, caspase inhibitors, cell crowding and different media. Exposure of the normal CD34(+) cells to different concentrations of GM-CSF and granulocyte colony-stimulating factor (G-CSF) increased apoptosis at lower concentrat...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

ABC chromogenic agar: a cost-effective alternative to standard enteric media for Salmonella spp. isolation from routine stool samples.
Authors: O'Neill W, Cooke RPD, Plumb H, Kennedy P Abstract Salmonellosis is the second most common cause of bacterial gastroenteritis, yet the yield from routine stool culture is low. Commonly used selective enteric media have poor specificities for salmonella identification, resulting in a high laboratory workload. A special chromogenic medium, ABC agar, is a promising alternative but its cost-effectiveness has not been evaluated in diagnostic laboratories. A collaborative study is therefore undertaken in two district general hospitals laboratories. Routine stool samples (n=866) were subcultured onto ABC agar half...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Biomedical Science in Brief.
Authors: Clarke SC, Reid JA, Thom L, Diggle MA, Mcchlery S, Scott K, Edwards GFS, Mcknight AJ, Shaw A, Goldsmith CE, Clarke L, Millar BC, Mccaughan J, Elborn JE, Reid A, Moore JE, Wu FS, Juan CW, Huang SM, Chang YJ, Chou -L, Siebers R, Mitsuhashi N, Matsuda M, Murayama O, Millar BC, Moore JE, Memish ZA, Balkhy HH, Francis C, Cunningham G, Hajeer AH, Almuneef MA PMID: 28696915 [PubMed] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Infections with Corynebacterium diphtheriae: six years' experience at an inner London teaching hospital.
This study presents data on 85 isolates of C. diphtheriae over a six year period (1998-2003). Eighty were non-toxigenic isolates from patients with sore throat, and five (one toxigenic) were from cutaneous ulcers in travellers returning from endemic areas. When examined in relation to denominator data provided by the Health Protection Agency (HPA) for the whole of England and Wales, 1998-2002, 75% of all notifications of C. diphtheriae for England and Wales originated from the laboratories at University College London Hospitals (UCLH). In some years (1999 and 2001) 95-100% of isolates came from UCLH. We believe that nation...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Synthetic organic hard capsule colouring agents: in vitro effect on human true and pseudo-cholinesterases.
In this study, the effects of three colouring agents (sunset yellow, quinoline yellow and erythrosine) on true and pseudo-cholinesterases (ChE) are assessed in erythrocytes and plasma, respectively. Results indicated that the synthetic compounds affected both true and pseudo ChE activity. The concentration of sunset yellow which caused 50% inhibition (IC50) of true ChE was about 64% that of pseudo-ChE; for erythrosine, IC50 was approximately the same for both true and pseudo-ChE; and for quinoline yellow, IC50 for true ChE was 25% of pseudo-ChE, although its effect on both true and pseudo-ChE was greater than seen with the...
Source: British Journal of Biomedical Science - July 14, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Erratum.
Authors: PMID: 28664818 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 2, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Referees 2016.
Authors: PMID: 28664819 [PubMed - in process] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - July 2, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Uncertainty of measurement in andrology: UK best practice guideline from the association of biomedical andrologists.
Authors: Sanders D, Fensome-Rimmer S, Woodward B Abstract Uncertainty of measurement has become a paramount factor to consider in pathology. In the UK, consideration of uncertainty of measurement is mandatory for medical laboratories who apply to be accredited against ISO15189:2012 via the United Kingdom Accreditation Service. This guideline intends to help those working within diagnostic andrology to better understand the concept of uncertainty, and how it can be applied to semen analysis and post-vasectomy semen analysis. The various areas where uncertainty may exist are identified, and guidance is provided to mi...
Source: British Journal of Biomedical Science - June 29, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Therapeutic warfarin use and the extrahepatic functions of vitamin K-dependent proteins.
Authors: Donaldson CJ, Harrington DJ Abstract The impact of warfarin therapy on the functions of extrahepatic vitamin K-dependent proteins (VKDP) is less clearly understood and less widely recognised in clinical practice than that on the hepatic counterparts (clotting factors II, VII, IX and X). Warfarin inhibits osteocalcin, an abundant extrahepatic VKDP involved in the mineralisation and maturation of bone and thus, primarily by this mechanism, may have an adverse effect on bone health. Whilst some studies do link warfarin use to an increase in osteoporosis and fracture risk others have not. Warfarin also inhibit...
Source: British Journal of Biomedical Science - June 29, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Evaluation of the Xpert Norovirus assay for the rapid detection of norovirus genogroups I and II in faecal specimens within a routine laboratory setting.
Authors: Cleary O, McNerney R, Yandle Z, O'Sullivan CE PMID: 28636465 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 22, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Erratum.
Authors: PMID: 28636466 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 22, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Toxicology in clinical laboratories: challenging times.
Authors: Brown NW Abstract In recent years, there have been a number of significant developments in toxicology within clinical laboratories, both with the available instrumentation and in the range of compounds abused by the drug using communities. There have also been developments in the regulation of forensic science in the UK which may in time impact clinical toxicology. This review is designed to provide an update of these changes within toxicology to the more general pathology laboratory audience. For detailed information in specific areas, the reader is referred to the references in the text. In the preparati...
Source: British Journal of Biomedical Science - June 21, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

SEPP1 and SEP15 gene polymorphisms and susceptibility to breast cancer.
Authors: Mohammaddoust S, Salehi Z, Saeidi Saedi H PMID: 28598259 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - June 11, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A review of the use of human papilloma virus (HPV) in cervical screening.
Authors: Crossley B, Crossley J Abstract Using key words online databases were searched to identify relevant publications to review the use of Human papilloma virus (HPV) in cervical screening. The mode of cervical screening in the UK has been decided but implementation plans have yet to be announced. The protracted uncertainty surrounding the initial announcement to move to HPV primary screening together with the lack of a national steer has resulted in a flight of staff which threatens the provision of the current and future service. The transition will be a challenging time but analysis of data from more than 17...
Source: British Journal of Biomedical Science - June 8, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Association of heterogenicity of Helicobacter pylori cag pathogenicity island with peptic ulcer diseases and gastric cancer.
Authors: Khatoon J, Prasad KN, Prakash Rai R, Ghoshal UC, Krishnani N Abstract OBJECTIVE: To investigate the frequency and integrity of certain cag pathogenicity island genes (cagPAI) in Helicobacter pylori strains and their association with peptic ulcer disease (PUD) and gastric cancer. MATERIAL AND METHODS: We enrolled 240 adult patients [120 with functional dyspepsia (FD), 50 with PUD and 70 with gastric cancer] undergoing upper gastrointestinal endoscopy. H. pylori infection was diagnosed when either culture or any two of the three tests (rapid urease test, histopathology and specific ureA PCR) were positiv...
Source: British Journal of Biomedical Science - June 4, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

Pleural fluid DNA integrity index as a diagnostic marker of malignant pleural effusion.
Authors: Eltorgoman AE, Badr E, Kombr Y, Yousif M PMID: 28537492 [PubMed - as supplied by publisher] (Source: British Journal of Biomedical Science)
Source: British Journal of Biomedical Science - May 26, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

A magnetic nanoparticle-labeled immunoassay with europium and samarium for simultaneous quantification of serum pepsinogen I and II.
Authors: Fan J, Xiao H, Zhang J, Zhou B, Deng L, Zhang Y, Huang B Abstract OBJECTIVE: To develop a novel immunoassay for the simultaneous determination of serum pepsinogen I (PG I) and pepsinogen II (PG II) by combining established methods of time-resolved fluoroimmunoassay (TRFIA) and magnetic nanoparticles separation. MATERIALS AND METHODS: This new immunoassay method was characterised by immobilising primary antibodies on the surface of magnetic particles and labelled with stable fluorescent chelates of europium and samarium. RESULTS: Using magnetic nanoparticles, the TRFIA immunoassay exhibited broad dy...
Source: British Journal of Biomedical Science - May 20, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

The clinical value of Vav3 in peripheral blood for predicting lymphatic metastasis of gastric cancer.
Authors: Tan B, Li Y, Wang C, Tan M, Fan L, Zhao Q, Wang D, Jia N Abstract BACKGROUND: Overexpression of Vav3, a gene involved in signal transduction, promotes invasion and inhibits apoptosis in several cancers. The clinical value of the protein product of this gene, Vav3, in the peripheral blood of gastric cancer patients is unknown. We hypothesised increased serum Vav3 that related to tissue levels and lymph node metastases. In addition, we further explored its clinical value in respect of linked molecules Rac-1, MMP-7 and ICAM-1 Methods: 120 gastric cancer patients who had radical surgery were enrolled. Immunohi...
Source: British Journal of Biomedical Science - May 19, 2017 Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research