Accuracy and prognostic ability of the SARC-F questionnaire and Ishii's score in the screening of sarcopenia in geriatric inpatients.
This study aimed to identify the cutoff values of the SARC-F questionnaire and Ishii's score using the variables age, grip strength, and calf circumference in older inpatients in China to compare the accuracy of the two methods and to explore their predictive ability for adverse outcomes (rehospitalization, falls, fracture, and death). Hospitalized patients (n=138) aged ≥60 years were included. The accuracy of the two tools was evaluated using the reference diagnosis recommended by the Asian Working Group on Sarcopenia (assessing patients with measurements of muscle mass, handgrip strength, and usual gait speed). Follow...
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Molecular detection of Leishmania spp in Lutzomyia longipalpis in the city of Lavras, Minas Gerais, Brazil.
Authors: Castro JC, Bueno LL, Milagres TF, Rêgo FD, Gontijo CMF, Peconick AP, Andrade AJ, Barçante TA, Barçante JMP Abstract Leishmaniasis is a neglected disease that affects a large part of the world population. Knowing the sand fly fauna of a region is of fundamental importance for guiding health surveillance actions related to the prevention and control of leishmaniasis. A total of 86 specimens of sand flies (60 females and 26 males) were collected. Using the classification proposed by Galati (2003), the following species were identified: Lutzomyia longipalpis (Lutz & Neiva, 1912), Migone...
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Validation of the two-minute step test in obese with comorbibities and morbidly obese patients.
Authors: Ricci PA, Cabiddu R, Jürgensen SP, André LD, Oliveira CR, Di Thommazo-Luporini L, Ortega FP, Borghi-Silva A Abstract Cardiopulmonary fitness assessment is a valuable resource to obtain quantitative indicators of an individual's physical performance. The cardiopulmonary exercise test (CPX), considered the gold standard test for this evaluation, is costly and difficult to be accessed by the general population. In order to make this evaluation more accessible, and to better reflect the performance of daily life activities, alternative tests were proposed. Morbidly obese patients present limitation...
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

The E3 ligase MuRF2 plays a key role in the functional capacity of skeletal muscle fibroblasts.
Authors: Silvestre JG, Baptista IL, Silva WJ, Cruz A, Silva MT, Miyabara EH, Labeit S, Moriscot AS Abstract Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might funct...
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Evaluation of volume responsiveness by pulse pressure variability and inferior vena cava dispensability index at different tidal volumes by mechanical ventilation.
This study investigated the effects of tidal volume (TV) on the diagnostic value of pulse pressure variation (PPV) and the inferior vena cava dispensability index (IVC-DI) for volume responsiveness during mechanical ventilation. In patients undergoing elective surgery with mechanical ventilation, different TVs of 6, 9, and 12 mL/kg were given for two min. The left ventricular outflow tract velocity-time integral (VTI) was measured by transthoracic echocardiography. The IVC-DI was measured at sub-xyphoid transabdominal long axis. The PPV was measured via the radial artery and served as baseline. Index measurements were repe...
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology.
This article presents the results of discussions within the Department of Biochemistry, University of São Paulo, on this subject. PMID: 31482979 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Local treatment with ascorbic acid accelerates recovery of post-sutured Achilles tendon in male Wistar rats.
Authors: Souza M, Moraes SAS, de Paula DR, Maciel AA, Batista EJO, Silva DGF, Bahia CP, Oliveira KRHM, Herculano AM Abstract Tendon rupture is a very frequent accident involving average people and high-performance athletes. Clinical studies describe tendon recovery as a painful and slow process involving different biochemical and histological events. Ascorbic acid (AA) is a potent antioxidant as well as an important cofactor for collagen synthesis. In the current study, we evaluated if local treatment with AA is able to promote tendon repair in tenotomized rats. Animals were submitted to Achilles tendon rupture fol...
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Pulmonary vein parameters are similar or better predictors than left atrial diameter for paroxysmal atrial fibrillation after cryoablation.
Authors: Li B, Ma H, Guo H, Liu P, Wu Y, Fan L, Cao Y, Jian Z, Sun C, Li H Abstract Left atrial diameter (LAD) has been considered an independent risk factor for atrial fibrillation (AF) relapse after pulmonary vein isolation (PVI). However, whether LAD or other factors are more predictive of late recurrence in patients with paroxysmal AF remains unclear. We aimed to evaluate the value of pulmonary vein (PV) parameters for predicting AF relapse 1 year after patients underwent cryoablation for paroxysmal AF. Ninety-seven patients with paroxysmal AF who underwent PVI successfully were included. PV parameters were mea...
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

A newly designed intensive caregiver education program reduces cognitive impairment, anxiety, and depression in patients with acute ischemic stroke.
In conclusion, ICEP effectively reduced cognitive impairment, anxiety, and depression in AIS patients. PMID: 31483000 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - September 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Diagnostic value of circulating lncRNA ANRIL and its correlation with coronary artery disease parameters.
This study aimed to detect the expression of the long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) and evaluate its correlation with disease risk, stenosis degree, inflammation, as well as overall survival (OS) in coronary artery disease (CAD) patients. A total of 230 patients who underwent diagnostic coronary angiography were consecutively recruited and assigned to CAD group (n=125) or control group (n=105) according to presence or absence of CAD. Gensini score was calculated to assess the severity of coronary artery damage. Plasma samples were collected and the expression ANRIL was detected ...
Source: Brazilian Journal of Medical and Biological Research - August 15, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts.
Authors: Braghirolli DI, Caberlon B, Gamba D, Petry J, Dias ML, Pranke P Abstract Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate-co-L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios ...
Source: Brazilian Journal of Medical and Biological Research - August 15, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effects of natural aging and gender on pro-inflammatory markers.
In conclusion, the natural aging process increased IL-6 and hsCRP levels, which is consistent with the inflammaging theory; however, women presented stronger correlations compared to men (IL-6 and hsCRP) and the 51-60 age range seems to be a key point for these increases. These findings are important because they indicate that early preventive measures may minimize the increase in these inflammatory markers in natural human aging. PMID: 31411315 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - August 15, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Ginsenoside Rd inhibits IL-1 β-induced inflammation and degradation of intervertebral disc chondrocytes by increasing IL1RAP ubiquitination.
In this study, chondrocytes were isolated from the nucleus pulposus tissues, and the cells were treated with ginsenoside compounds and IL-1β, alone and in combination. Cell viability and death rate were assessed by CCK-8 and flow cytometry methods, respectively. PCR, western blot, and immunoprecipitation assays were performed to determine the mRNA and protein expression, and the interactions between proteins, respectively. Monomeric component of ginsenoside Rd had no toxicity at the tested range of concentrations. Furthermore, Rd suppressed the inflammatory response of chondrocytes to interleukin (IL)-1β by suppr...
Source: Brazilian Journal of Medical and Biological Research - August 15, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Downregulation of lncRNA CCDC26 contributes to imatinib resistance in human gastrointestinal stromal tumors through IGF-1R upregulation.
In this study, we investigated the association between lncRNA CCDC26 and IGF-1R in GIST and their involvement in drug resistance. Considering the key role of lncRNAs in drug resistance in cancer, we hypothesized that IGF-1R is regulated by lncRNAs. The expression of a series of reported drug resistance-related lncRNAs, including CCDC26, ARF, H19, NBR2, NEAT1, and HOTAIR, in GIST cells treated with imatinib H19 was examined at various time-points by qRT-PCR. Based on our results and published literature, CCDC26, a strongly down-regulated lncRNA following imatinib treatment, was chosen as our research target. GIST cells with...
Source: Brazilian Journal of Medical and Biological Research - June 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effect of intraoperative lung-protective mechanical ventilation on pulmonary oxygenation function and postoperative pulmonary complications after laparoscopic radical gastrectomy.
This study aimed to observe the effects of lung-protective ventilation (LPV) on oxygenation index (OI) and postoperative pulmonary complications (PPCs) after laparoscopic radical gastrectomy in middle-aged and elderly patients. A total of 120 patients who were scheduled to undergo laparoscopic radical gastrectomy with an expected time of>3 h were randomly divided into conventional ventilation (CV group) with tidal volume (TV) of 10 mL/kg without positive end-expiratory pressure (PEEP), and lung-protective ventilation (PV group) with 7 mL/kg TV and personal level of PEEP with regular recruitment maneuver every 30 min. Me...
Source: Brazilian Journal of Medical and Biological Research - June 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Are experienced and high-level race walking athletes able to match pre-programmed with executed pacing?
The objective of this study was to verify the agreement between pre-programmed and executed pacing during race walking and whether level of the athletes experience and performance influenced this relationship. Twenty-nine national and international race walkers participated in this study (14 males, 24.0±7.1 years old, and 15 females, 23.3±7.3 years old). Pre-programmed pacing for 10- and 20-km official walking races was self-selected via demonstrative pacing charts prior to races, while executed pacing was analyzed by a specialist investigator via an individual plot of current velocity versus distance. There ...
Source: Brazilian Journal of Medical and Biological Research - June 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Expression of myo-inositol cotransporters in the sciatic nerve and dorsal root ganglia in experimental diabetes.
Authors: Farias VX, Uchoa PN, Aquino CP, Britto LRG, Fonteles MC, Leal-Cardoso JH, Silva-Alves KS, Havt A, Prata MMG, Heimark DB, Nascimento NRF, Santos CF Abstract The transport of myo-inositol is the main mechanism for the maintenance of its high intracellular levels. We aimed to measure the mRNA and protein levels of myo-inositol cotransporters in the sciatic nerve (SN) and dorsal root ganglia (DRG) during experimental diabetes. Streptozotocin-induced (STZ; 4, 8, and 12 weeks; 65 mg/kg; ip) diabetic rats (DB) and age-matched euglycemic (E) rats were used for the analysis of mRNA and protein levels of sodium myo-...
Source: Brazilian Journal of Medical and Biological Research - June 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Cardiac function and intracellular Ca2+ handling proteins are not impaired by high-saturated-fat diet-induced obesity.
In conclusion, the hypothesis was not confirmed, since the high-saturated-fat diet-induced obese rats did not present cardiac dysfunction or impaired intracellular Ca2+ handling proteins. PMID: 31141087 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - May 30, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma.
Authors: Yu CH, Zhao JS, Zhao H, Peng T, Shen DC, Xu QX, Li Y, Webb RC, Wang MH, Shi XM, Peng C, Ding WJ Abstract The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determin...
Source: Brazilian Journal of Medical and Biological Research - May 30, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Pancreatic β-cell function is inhibited by miR-3666 in type 2 diabetes mellitus by targeting adiponectin.
In conclusion, our results revealed that miR-3666 inhibited pancreatic cell proliferation, reduced insulin sensitivity, and promoted apoptosis by targeting ADIPOQ. PMID: 31141089 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - May 30, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

CCAAT/enhancer-binding protein alpha (CEBPA) gene haploinsufficiency does not alter hematopoiesis or induce leukemia in Lck-CALM/AF10 transgenic mice.
Authors: Lange AP, Almeida LY, Araújo Silva CL, Scheucher PS, Chahud F, Krause A, Bohlander SK, Rego EM Abstract Although rare, CALM/AF10 is a chromosomal rearrangement found in immature T-cell acute lymphoblastic leukemia (T-ALL), acute myeloid leukemia, and mixed phenotype acute leukemia of T/myeloid lineages with poor prognosis. Moreover, this translocation is detected in 50% of T-ALL patients with gamma/delta T cell receptor rearrangement, frequently associated with low expression of transcription factor CCAAT/enhancer-binding protein alpha (CEBPA). However, the relevance of CEBPA low expression for CALM...
Source: Brazilian Journal of Medical and Biological Research - May 30, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Linderae radix ethanol extract attenuates alcoholic liver injury via attenuating inflammation and regulating gut microbiota in rats.
This study aimed to explore the influence of gut microbiota alterations induced by Linderae radix ethanol extract (LREE) on alcoholic liver disease (ALD) in rats and to study the anti-inflammatory effect of LREE on ALD through the lipopolysaccharide (LPS) toll-like receptor 4 (TLR4)-nuclear factor kappa B (NF-κB) pathway. ALD rat models were established by intragastric liquor [50% (v/v) ethanol] administration at 10 mL/kg body weight for 20 days. Rats were divided into six groups: normal group (no treatment), model group (ALD rats), Essentiale group (ALD rats fed with Essentiale, 137 mg/kg), and LREE high/moderate/lo...
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Moderate-intensity exercise allows enhanced protection against oxidative stress-induced cardiac dysfunction in spontaneously hypertensive rats.
This study aimed to investigate whether moderate-intensity exercise (MIE) could improve cardiac function and oxidative stress in spontaneously hypertensive rats (SHRs). Eight-week-old male SHRs and age-matched male Wistar-Kyoto rats were randomly assigned to exercise training (treadmill running at a speed of 20 m/min for 1 h continuously) or kept sedentary for 16 weeks. Cardiac function was monitored by polygraph; cardiac mitochondrial structure was observed by scanning electron microscope; tissue free radical production was measured using dihydroethidium staining. Expression levels of SIRT3 and SOD2 protein were measured ...
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Evaluation of the effects of Uncaria rhynchophylla alkaloid extract on LPS-induced preeclampsia symptoms and inflammation in a pregnant rat model.
Authors: Wu LZ, Xiao XM Abstract Excessive pro-inflammatory cytokines result in adverse pregnancy outcomes, including preeclampsia-like phenotypes, and fetal growth restriction. Anti-inflammation might be an effective therapy. The aim of this research was to investigate whether Uncaria rhynchophylla alkaloid extract (URE), a highly safe anti-inflammation constituent of the herb, can inhibit inflammation and improve clinical characteristics of preeclampsia in a lipopolysaccharide (LPS)-induced preeclampsia rat model. The rat model was established by daily administration of LPS (1 μg/kg body weight per day) from g...
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Antinociceptive and anti-inflammatory activities of a triterpene-rich fraction from Himatanthus drasticus.
The objective of this study was to investigate a triterpene-rich fraction (FJNB) from H. drasticus latex on acute models of nociception and inflammation and to clarify its mechanisms of action. Wistar rats or Swiss mice were subjected to the carrageenan-induced paw edema test or the formalin test, respectively, after the acute oral treatment with FJNB. The inflamed paws from the carrageenan-induced paw edema and formalin tests were processed for histological and immunohistochemical assays, respectively. The results were analyzed by ANOVA and considered significant at P
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Segmental resection combined with anoplasty for the treatment of circumferential mixed hemorrhoids.
Authors: Wu J, Yu K, Lv C, Lu W, He H Abstract Circumferential mixed hemorrhoids are very difficult to treat non-surgically. Therefore, it is important to explore the surgical methods for its complete resolution as well as maintenance of normal anal anatomy and function. The present study was designed to evaluate the effect of segmented and plastic hemorrhoidectomy (SPH) on patients with circumferential mixed hemorrhoids. A total of 300 patients with circumferential mixed hemorrhoids were divided into experimental group (n=150) undergoing SPH and control group (n=150) undergoing Milligan-Morgan hemorrhoidectomy. Th...
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Impact of enhanced recovery after surgery on postoperative rehabilitation, inflammation, and immunity in gastric carcinoma patients: a randomized clinical trial.
Authors: Wang WK, Tu CY, Shao CX, Chen W, Zhou QY, Zhu JD, Xu HT Abstract We determined the effects of enhanced recovery after surgery (ERAS) in patients undergoing radical surgery for gastric carcinoma. Sixty patients undergoing radical gastrectomy for gastric carcinoma in Lishui Hospital between March and October 2016 were randomized to receive either ERAS (30 patients) or conventional care (30 patients, controls). Clinical, economic, and laboratory indices were analyzed. ERAS patients showed faster recovery and shorter postoperative hospital stays than the controls (P
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

The ERBB2 gene polymorphisms rs2643194, rs2934971, and rs1058808 are associated with increased risk of gastric cancer.
Authors: Vázquez-Ibarra KC, Bustos-Carpinteyro AR, García-Ruvalcaba A, Magaãa-Torres MT, Gutiérrez-Aguilar R, Marín-Contreras ME, Santiago-Luna E, Sánchez-López JY Abstract Gastric cancer (GC) is the third most lethal type of cancer worldwide. Single nucleotide polymorphisms (SNPs) in regulatory sites or coding regions can modify the expression of genes involved in gastric carcinogenesis, as ERBB2, which encodes for the tyrosine-kinase receptor HER-2. The aim of this work was to analyze the association of the polymorphisms: rs2643194, rs2517951, rs2643195, rs2934971,...
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Two new mixed-ligand coordination polymers based on multi-N chelating ligand inhibit YAP expression and induce caspase-mediated spinal tumor cell apoptosis.
Authors: Sun YF, Shao LW, Chen Q, Gao X, Li F, Wu CY Abstract Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6'-bis-(1H-benzoimidazol-2-yl)-2,2'-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The c...
Source: Brazilian Journal of Medical and Biological Research - May 23, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Association between tumor necrosis factor polymorphisms and rheumatoid arthritis as well as systemic lupus erythematosus: a meta-analysis.
Authors: Chen L, Huang Z, Liao Y, Yang B, Zhang J Abstract Tumor necrosis factor-alpha (TNF-α) plays an important role in autoimmune diseases. Previous studies have investigated the association of TNF-α-238G/A (rs361525) and -308G/A (rs1800629) polymorphisms with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). However, no agreed conclusion had been made. Therefore, this meta-analysis was conducted to assess the associations of TNF-α-238G/A and -308G/A polymorphisms with RA and SLE risk. A systematic search was conducted in commonly used databases. Meta-analysis was performed by S...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Prognostic factors in patients undergoing early-start peritoneal dialysis within 24 h after catheter insertion.
This study aimed to investigate the clinical characteristics, prognosis, and factors for survival of patients who underwent early-start peritoneal dialysis (PD) within 24 h after catheter insertion three years after PD. This study was conducted from January 1, 2013 to December 31, 2017. All adult patients who were diagnosed with end-stage renal disease (ESRD) and underwent PD for the first time within 24 h after catheter insertion in our hospital were included. All patients with PD were followed-up until they withdrew from PD, switching to hemodialysis, were transferred to other medical centers, underwent renal transplanta...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method.
This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse blood with K. pneumoniae were used for sensitivity analysis. The detection limit of the LAMP was 1 bacterium/reaction. The results showed t...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effects of artepillin C on model membranes displaying liquid immiscibility.
Authors: Pazin WM, Vilanova N, Voets IK, Soares AEE, Ito AS Abstract It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and giant unilamelar vesicles) composed of ternary lipid mixtures containing cholesterol, which display liquid-ordered (lo) and liquid-disordered (ld) phase coexistence. Specifically, we explored po...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Burden, access, and disparities in kidney disease.
This article was published in Kidney International volume 95, pages 242-248, https://doi.org/10.1016/j.kint.2018.11.007, Copyright World Kidney Day 2019 Steering Committee (2019) and is reprinted concurrently in several journals. The articles cover identical concepts and wording, but vary in minor stylistic and spelling changes, detail, and length of manuscript in keeping with each journal's style. Any of these versions may be used in citing this article. Note that all authors contributed equally to the conception, preparation, and editing of the manuscript. Kidney disease is a global public health problem, affecting over ...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Erratum for: "Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation" [Braz J Med Biol Res (2018) 51(10): e7439].
Erratum for: "Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation" [Braz J Med Biol Res (2018) 51(10): e7439]. Braz J Med Biol Res. 2019 02 25;52(2):e7439erratum Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20187439]. PMID: 30810603 [PubMed] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Observation of the effect of bone marrow mesenchymal stem cell transplantation by different interventions on cirrhotic rats.
Authors: Zhou X, Yang J, Liu Y, Li Z, Yu J, Wei W, Chen Q, Li C, Tang N Abstract Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte ...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Protective effect of dexmedetomidine against organ dysfunction in a two-hit model of hemorrhage/resuscitation and endotoxemia in rats.
Authors: Jiang Y, Xia M, Huang Q, Ding D, Li Y, Zhang Z, Zhang X Abstract Dexmedetomidine (DEX), a selective agonist of α2-adrenergic receptors, has anti-inflammation properties and potential beneficial effects against trauma, shock, or infection. Therefore, this study aimed to investigate whether DEX might protect against multiple-organ dysfunction in a two-hit model of hemorrhage/resuscitation (HS) and subsequent endotoxemia. Eighty Wistar rats were randomized into four groups: NS (normal saline), HS/L (HS plus lipopolysaccharide), HS/L+D (HS/L plus dexmedetomidine), and HS/L+D+Y (HS/L+D plus yohimbine). Si...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Emodin protects H9c2 cells from hypoxia-induced injury by up-regulating miR-138 expression.
Authors: Zhang X, Qin Q, Dai H, Cai S, Zhou C, Guan J Abstract Myocardial infarction (MI) is a common presentation for ischemic heart disease, which is a leading cause of death. Emodin is a Chinese herbal anthraquinone used in several diseases. However, the effect of emodin in hypoxia-induced injury in cardiomyocytes has not been clearly elucidated. Our study aimed to clarify the functions of emodin in hypoxia-induced injury in rat cardiomyocytes H9c2 and explore the underlying mechanism. The effects of emodin on cell viability and apoptosis were analyzed by the Cell counting kit-8 assay and flow cytometry assay, r...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Visual and automatic classification of the cyclic alternating pattern in electroencephalography during sleep.
Authors: Largo R, Lopes MC, Spruyt K, Guilleminault C, Wang YP, Rosa AC Abstract Cyclic alternating pattern (CAP) is a neurophysiological pattern that can be visually scored by international criteria. The aim of this study was to verify the feasibility of visual CAP scoring using only one channel of sleep electroencephalogram (EEG) to evaluate the inter-scorer agreement in a variety of recordings, and to compare agreement between visual scoring and automatic scoring systems. Sixteen hours of single-channel European data format recordings from four different sleep laboratories with either C4-A1 or C3-A2 channels and...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effect of risperidone on proliferation and apoptosis of MC3T3-E1 cells.
Authors: Zheng L, Yang L, Zhao X, Long N, Li P, Wang Y Abstract This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprote...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Amifostine reduces inflammation and protects against 5-fluorouracil-induced oral mucositis and hyposalivation.
This study investigated the protective effect of amifostine on an experimental model of OM. Hamsters were divided into six groups: saline control group (5 mL/kg), mechanical trauma (scratches) of the right cheek pouch; 5-FU (60 and 40 mg/kg, ip, respectively, administered on days 1 and 2); amifostine (12.5, 25, or 50 mg/kg) + 5-FU + scratches. Salivation rate was assessed and the animals were euthanized on day 10 for the analysis of macroscopic and microscopic injury by scores. Tissue samples were harvested for the measurement of neutrophil infiltration and detection of inflammatory markers by ELISA and immunohistochemistr...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Cirrhotic cardiomyopathy: the liver affects the heart.
Authors: Carvalho MVH, Kroll PC, Kroll RTM, Carvalho VN Abstract Cirrhotic cardiomyopathy historically has been confused as alcoholic cardiomyopathy. The key points for diagnosis of cirrhotic cardiomyopathy have been well explained, however this entity was neglected for a long time. Nowadays the diagnosis of this entity has become important because it is a factor that contributes significantly to morbidity-mortality in cirrhotic patients. Characteristics of cirrhotic cardiomyopathy are a hyperdynamic circulatory state, altered diastolic relaxation, impaired contractility, and electrophysiological abnormalities, par...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Kaempferol suppresses human gastric cancer SNU-216 cell proliferation, promotes cell autophagy, but has no influence on cell apoptosis.
This study aimed to investigate the effects of kaempferol on gastric cancer SNU-216 cell proliferation, apoptosis, and autophagy, as well as underlying potential mechanisms. Viability, proliferation, and apoptosis of SNU-216 cells after kaempferol treatment were evaluated using cell counting kit-8 assay, 5-btomo-2'-deoxyuridine incorporation assay, and annexin V-FITC/PI staining, respectively. Quantitative reverse transcription PCR was performed to measure the mRNA expressions of cyclin D1 and microRNA-181a (miR-181a) in SNU-216 cells. Cell transfection was used to down-regulate the expression of miR-181a. The protein expr...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Serotonin induces or inhibits neuritic regeneration of leech CNS neurons depending on neuronal identity.
Authors: Vargas J, Alfaro-Rodríguez A, Perez-Orive J Abstract Recovery of motor function after central nervous system (CNS) injury is dependent on the regeneration capacity of the nervous system, which is a multifactorial process influenced, among other things, by the role of neuromodulators such as serotonin. The neurotransmitter serotonin can promote neuronal regeneration but there are also reports of it causing restriction, so it is important to clarify these divergent findings in order to understand the direct scope and side effects of potential pharmacological treatments. We evaluated the effect of sero...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples.
Authors: Santos MFM, Oliveira FCAC, Kishimoto RK, Borri D, Santos FPS, Campregher PV, Silveira PAA, Hamerschlak N, Mangueira CLP, Duarte FB, Crepaldi AH, Salvino MA, Velloso EDRP Abstract Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndro...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications.
In conclusion, the VOB formulation contributed to the improvement of current therapeutic strategies for cutaneous applications in skin care. PMID: 30785481 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Thyroid disorders in Brazil: the contribution of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil).
Authors: Bensenor IM Abstract Thyroid disorders are common diseases, both in Brazil and worldwide. The Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) is a prospective cohort study that investigates cardiovascular diseases, diabetes, and associated factors, including non-classical cardiovascular risk factors such as thyroid function. Thyroid function was classified according to thyrotropin stimulating hormone (TSH), free thyroxine (FT4), and use of medication to treat thyroid disorders, after excluding participants who reported use of any medication that could alter the results of the TSH and FT4 tests. ...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Erratum for: "Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II" [Braz J Med Biol Res (2018) 51(12): e7526].
Erratum for: "Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II" [Braz J Med Biol Res (2018) 51(12): e7526]. Braz J Med Biol Res. 2019 Jan 10;52(2):e7526erratum Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20187526]. PMID: 30726313 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - February 8, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

CD47 expression is decreased in hematopoietic progenitor cells in patients with myelofibrosis.
Authors: Nonino A, Nascimento JM, Mascarenhas CC, Mazzeu JF, Pereira RW, Jacomo RH Abstract Myelofibrosis (MF) is characterized by increased circulating hematopoietic progenitor cells (HPCs), abnormal cytokine levels, and the survival advantage of neoplastic progenitors over their normal counterparts, which leads to progressive disappearance of polyclonal hematopoiesis. CD47 is a surface glycoprotein with many functions, such as acting as a phagocytosis inhibitor of the expressing cell, that is increased in normal hematopoietic stem and progenitor cells mobilized into the blood and several human cancer-initiating c...
Source: Brazilian Journal of Medical and Biological Research - December 13, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Voluntary wheel running: patterns and physiological effects in mice.
Authors: Manzanares G, Brito-da-Silva G, Gandra PG Abstract Exercise can prevent and improve the pathophysiology of diseases and promote healthy aging. Thus, understanding the mechanisms that regulate the beneficial effects of exercise may lead to the development of new strategies to enhance quality of life and to counteract chronic diseases. Voluntary wheel running is an interesting model to study the effects of exercise in mice. Compared to forced treadmill exercise, voluntary wheel running presents several advantages such as: 1) running pattern is similar to natural running behavior of mice; 2) it is performed u...
Source: Brazilian Journal of Medical and Biological Research - December 13, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research