Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2.2.15 cells by targeting Lin28a/let-7a axis.
Authors: Qiu Z, Zhou J, Zhang C, Cheng Y, Hu J, Zheng G Abstract An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induce...
Source: Brazilian Journal of Medical and Biological Research - May 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Efficacy, acceptability, and tolerability of antidepressant treatments for patients with post-stroke depression: a network meta-analysis.
Authors: Qin B, Chen H, Gao W, Zhao LB, Zhao MJ, Qin HX, Chen W, Chen L, Yang MX Abstract The aim of this study was to investigate the efficacy, acceptability, and tolerability of antidepressants in treating post-stroke depression (PSD) by performing a network meta-analysis of randomized controlled trials of the current literature. Eligible studies were retrieved from online databases, and relevant data were extracted. The primary outcome was efficacy as measured by the mean change in overall depressive symptoms. Secondary outcomes included discontinued treatment for any reason and specifically due to adverse event...
Source: Brazilian Journal of Medical and Biological Research - May 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Epoetin alfa resistance in hemodialysis patients with chronic kidney disease: a longitudinal study.
This study aimed to investigate the factors associated with resistance to recombinant human erythropoietin therapy with epoetin alfa (αEPO). We performed a prospective, longitudinal study of hemodialysis patients receiving treatment with αEPO at our reference hospital from July 2015 to June 2016. Clinical data was collected, and the response to αEPO treatment was evaluated using the erythropoietin resistance index (ERI). The ERI was defined as the weekly weight-adjusted αEPO dose (U/kg per week)/hemoglobin level (g/dL). A longitudinal linear regression model was fitted with random effects to verify ...
Source: Brazilian Journal of Medical and Biological Research - May 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

LncRNA HULC mediates radioresistance via autophagy in prostate cancer cells.
Authors: Chen C, Wang K, Wang Q, Wang X Abstract Prostate cancer (PCa) is the second leading cause of cancer death in men. Irradiation is one of the available options for treatment of PCa, however, approximately 10-45% of PCa are resistant to irradiation. We aimed to explore the role of long non-coding RNA highly upregulated in liver cancer (HULC) in the sensitivity of PCa cells to irradiation. Survival rate, cell apoptosis, cycle, expressions of related proteins, and caspase-3 activity were assessed to explore the effects of HULC on sensitivity of PCa cells to irradiation. Expression of HULC in DU-145, PC3, LNCaP,...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Neonatal tobacco smoke reduces thermogenesis capacity in brown adipose tissue in adult rats.
Authors: Peixoto TC, Moura EG, Oliveira E, Younes-Rapozo V, Soares PN, Rodrigues VST, Santos TR, Peixoto-Silva N, Carvalho JC, Calvino C, Conceição EPS, Guarda DS, Claudio-Neto S, Manhães AC, Lisboa PC Abstract Maternal smoking is a risk factor for progeny obesity. We have previously shown, in a rat model of neonatal tobacco smoke exposure, a mild increase in food intake and a considerable increase in visceral adiposity in the adult offspring. Males also had secondary hyperthyroidism, while females had only higher T4. Since brown adipose tissue (BAT) hypofunction is related to obesity, here we ...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Influence of expiratory positive airway pressure on cardiac autonomic modulation at rest and in submaximal exercise in COPD patients.
Authors: da L Goulart C, San Martin EA, Mansour KMK, Schneiders PB, da Silva ALG Abstract The aim of this study was to evaluate the effect of expiratory positive airway pressure (EPAP) on heart rate variability (HRV) indices at rest and during 6-min walk test (6MWT) in chronic obstructive pulmonary disease (COPD) patients. Fifteen moderate to severe COPD patients were randomized and evaluated with and without (Non-EPAP) a 5 cmH2O EPAP device. Respiratory rate (RR) was collected at rest (5 min), during the 6MWT (5 min), and at recovery (5 min). Indices of HRV were computed in the time domain, in the frequency domain...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

TNF- α inhibits SCF, ghrelin, and substance P expressions through the NF-κB pathway activation in interstitial cells of Cajal.
In conclusion, TNF-α down-regulated the expressions of SCF, ghrelin, and substance P via the activation of the NF-κB pathway in ICC. PMID: 29694505 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Dietary intake and eating behavior in depot medroxyprogesterone acetate users: a systematic review.
Authors: Silva P, Qadir S, Fernandes A, Bahamondes L, Peipert JF Abstract Because of weight gain, women often discontinue hormonal contraception, especially depot medroxyprogesterone acetate (DMPA). Our objective was to conduct a systematic review of studies describing dietary intake or eating behavior in DMPA users to understand whether the use of DMPA is associated with changes in dietary habits and behaviors leading to weight gain. We searched the PubMed, POPLINE, CENTRAL Cochrane, Web of Science, and EMBASE databases for reports published in English between 1980 and 2017 examining dietary intake or eating behav...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Protective effect of yerba mate intake on the cardiovascular system: a post hoc analysis study in postmenopausal women.
Authors: da Veiga DTA, Bringhenti R, Copes R, Tatsch E, Moresco RN, Comim FV, Premaor MO Abstract The prevalence of cardiovascular and metabolic diseases is increased in postmenopausal women, which contributes to the burden of illnesses in this period of life. Yerba mate (Ilex paraguariensis) is a native bush from Southern South America. Its leaves are rich in phenolic components, which may have antioxidant, vasodilating, hypocholesterolemic, and hypoglycemic proprieties. This post hoc analysis of the case-control study nested in the Obesity and Bone Fracture Cohort evaluated the consumption of yerba mate and the p...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Andrographolide protects mouse astrocytes against hypoxia injury by promoting autophagy and S100B expression.
Authors: Du J, Zhang C, Na X, Li A, Zhang Q, Li K, Ding Y Abstract Andrographolide (ANDRO) has been studied for its immunomodulation, anti-inflammatory, and neuroprotection effects. Because brain hypoxia is the most common factor of secondary brain injury after traumatic brain injury, we studied the role and possible mechanism of ANDRO in this process using hypoxia-injured astrocytes. Mouse cortical astrocytes C8-D1A (astrocyte type I clone from C57/BL6 strains) were subjected to 3 and 21% of O2 for various times (0-12 h) to establish an astrocyte hypoxia injury model in vitro. After hypoxia and ANDRO administratio...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPAR γ expression.
In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to eva...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Exploration of gene functions for esophageal squamous cell carcinoma using network-based guilt by association principle.
Authors: Wu W, Huang B, Yan Y, Zhong ZQ Abstract Gene networks have been broadly used to predict gene functions based on guilt by association (GBA) principle. Thus, in order to better understand the molecular mechanisms of esophageal squamous cell carcinoma (ESCC), our study was designed to use a network-based GBA method to identify the optimal gene functions for ESCC. To identify genomic bio-signatures for ESCC, microarray data of GSE20347 were first downloaded from a public functional genomics data repository of Gene Expression Omnibus database. Then, differentially expressed genes (DEGs) between ESCC patients an...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury.
In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. H9c2 cells and human cardiomyocytes (HCM) were subjected to either hypoxia/reoxygenation (H/R), I/R or normal conditions (normoxia). The expression levels of lncRNA-ROR were detected in serum of myocardial I/R injury patients, H9c2 cells, and HCM by qRT-PCR. Then, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were measured by kits. Cell viability, apoptosis, apoptosis-associated factors, and p38/MAPK pathway were examine...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Quality of life on hemodialysis and inflammation: a descriptive analysis.
Authors: Bacci MR, Adami F, Figueiredo FWS, Alves BCA, da Veiga GL, Fonseca FLA Abstract Chronic kidney disease (CKD) is highly prevalent worldwide. Patients with CKD on hemodialysis are more likely to present behavioral changes and worse quality of life as a result of their routine and complications. They also have higher levels of cytokines. The aim of this study is to assess the relationship between the inflammatory profile and quality of life measured by KDOQL-SF36 in hemodialysis outpatients. Patients older than 21 years of age and on routine hemodialysis for at least 6 months with treatment on a regular weekl...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

miR-27a protects human mitral valve interstitial cell from TNF- α-induced inflammatory injury via up-regulation of NELL-1.
This study aimed to investigate the functional impacts of miR-27a on TNF-α-induced inflammatory injury in human mitral valve interstitial cells (hMVICs). hMVICs were subjected to 40 ng/mL TNF-α for 48 h, before which the expressions of miR-27a and NELL-1 in hMVICs were altered by stable transfection. Trypan blue staining, BrdU incorporation assay, flow cytometry detection, ELISA, and western blot assay were performed to detect cell proliferation, apoptosis, and the release of proinflammatory cytokines. We found that miR-27a was lowly expressed in response to TNF-α exposure in hMVICs. Overexpression of miR...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Advancement in liver laparoscopic resection - development of a new surgical device.
Authors: Vavra P, Karnik L, Skrobankova M, Jurcikova J, Ihnat P, Zonca P, Peteja M, El-Gendi A, Czudek S Abstract Liver resection is the standard treatment for any liver lesion. Laparoscopic liver resection is associated with lower intra-operative blood loss and fewer complications than open resection. Access to the posterior part of the right liver lobe is very uncomfortable and difficult for surgeons due the anatomic position, especially when employing laparoscopic surgery. Based on these experiences, a new laparoscopic device was developed that is capable of bending its long axis and allowing the application of ...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells.
Authors: Rolim PM, Fidelis GP, Padilha CEA, Santos ES, Rocha HAO, Macedo GR Abstract Melon (Cucumis melo L.) has high economic value and in recent years, its production has increased; however, part of the fruit is wasted. Usually, inedible parts such as peel and seeds are discarded during processing and consumption. Extracts of melon residues were prepared and their phenolic compounds, antioxidants and antiproliferative activities were evaluated. Total phenolic compounds were found in hydroethanolic, hydromethanolic, and aqueous extracts, especially for melon peel (1.016 mg gallic acid equivalent/100 g). Flavonoids...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Urinary semaphorin 3A as an early biomarker to predict contrast-induced acute kidney injury in patients undergoing percutaneous coronary intervention.
This study enrolled 168 patients with stable angina undergoing elective PCI. Serial urine samples, obtained at baseline and 2, 6, 12, 24, 36, and 48 h post-PCI were analyzed by semaphorin 3A and neutrophil gelatinase-associated lipocalin (NGAL) ELISA kit. AKI was defined as an increase in serum creatinine beyond 50% according to the RIFLE classification system. Receiver operator characteristic (ROC) curve analyses identified optimal semaphorin 3A and NGAL values for diagnosing CI-AKI. CI-AKI occurred in 20 of 168 patients. There were no significant differences in the baseline clinical characteristics and angiographic findi...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Inflammatory biomarkers responses after acute whole body vibration in fibromyalgia.
In conclusion, a single acute session of mild and short WBV can improve the inflammatory status in patients with FM, reaching values close to those of matched HW at their basal status. The neuroendocrine mechanism seems to be an exercise-induced modulation towards greater adaptation to stress response in these patients. PMID: 29513791 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Polydatin inhibits cell proliferation, invasion and migration, and induces cell apoptosis in hepatocellular carcinoma.
Authors: Jiao Y, Wu Y, Du D Abstract Polydatin, a small molecule from Polygonum cuspidatum, has many biological functions, particularly anti-cancer effects. However, the anti-cancer effects of polydatin in hepatocellular carcinoma (HCC) have not been examined yet. In the present study, MTT assay, BrdU assay, transwell invasion assay, and wound healing assay were performed to determine cell proliferation, invasion and migration. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Quantitative real-time PCR and western blotting assays were used to determine mRNA and protein expression levels. Xenograf...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Tea polyphenols induce S phase arrest and apoptosis in gallbladder cancer cells.
Authors: Wang J, Pan Y, Hu J, Ma Q, Xu Y, Zhang Y, Zhang F, Liu Y Abstract Gallbladder cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-cancer effect of TPs in human gallbladder cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

The hepatic ectonucleotide pyrophosphatase/phosphodiesterase 1 gene mRNA abundance is reduced by insulin and induced by dexamethasone.
In conclusion, we demonstrated for the first time that hepatic Enpp1 is regulated in the cycle of fasting and refeeding, a process that might be attributed to insulin-reduced Enpp1 expression. This insulin-reduced Enpp1 expression might play a role in the development of complications in diabetic patients. PMID: 29513794 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Structural heart disease as the cause of syncope.
We described the clinical evolution of patients with structural heart disease presenting at the emergency room with syncope. Patients were stratified according to their syncope etiology and available scores for syncope prognostication. Cox proportional hazard models were used to investigate the relationship between etiology of the syncope and event-free survival. Of the 82,678 emergency visits during the study period, 160 (0.16%) patients were there due to syncope, having a previous diagnosis of structural heart disease. During the median follow-up of 33.8±13.8 months, mean age at the qualifying syncope event was 68...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Morphological changes in rat rectus abdominis muscle induced by diabetes and pregnancy.
Authors: Vesentini G, Marini G, Piculo F, Damasceno DC, Matheus SMM, Felisbino SL, Calderon IMP, Hijaz A, Barbosa AMP, Rudge MVC Abstract The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore,...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Treatment with ascorbic acid and α-tocopherol modulates oxidative-stress markers in the spinal cord of rats with neuropathic pain.
This study investigated the effect of intraperitoneal administration of vit. E (15 mg·kg-1·day-1) and vit. C (30 mg·kg-1·day-1), given alone or in combination, on spinal cord oxidative-stress markers in CCI rats. Adult male Wistar rats weighing 200-250 g were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve), which received injections of vitamins or vehicle (saline co...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice.
This study investigated the analgesic effects of an ethanol extract of M. androsaceus (MAE) and its potential molecular mechanisms. Oral administration of MAE (50, 200, and 1000 mg/kg) had significant analgesic effects in an acid-induced writhing test, a formalin test, and a hot-plate test, with effectiveness similar to tramadol (the positive control drug). The autonomic activity test showed that MAE had no harmful effects on the central nervous system in mice. MAE resulted in significantly enhanced levels of noradrenalin and 5-hydroxytryptamine in serum but suppressed both of these neurotransmitters in the hypothalamus af...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Ginsenoside Rg1 inhibits dietary-induced obesity and improves obesity-related glucose metabolic disorders.
In conclusion, our observations suggested that ginsenoside Rg1 inhibited dietary-induced obesity and improved obesity-related insulin resistance and glucose intolerance by activation of the AMPK pathway. PMID: 29513799 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Estrogen and progesterone promote breast cancer cell proliferation by inducing cyclin G1 expression.
Authors: Tian JM, Ran B, Zhang CL, Yan DM, Li XH Abstract Breast cancer is the most common cause of cancer among women in most countries (WHO). Ovarian hormone disorder is thought to be associated with breast tumorigenesis. The present study investigated the effects of estrogen and progesterone administration on cell proliferation and underlying mechanisms in breast cancer MCF-7 cells. It was found that a single administration of estradiol (E2) or progesterone increased MCF-7 cell viability in a dose-dependent manner and promoted cell cycle progression by increasing the percentage of cells in the G2/M phase. A comb...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus.
Authors: Gonçalves FZ, Lizarte Neto FS, Novais PC, Gattas D, Lourenço LG, de Carvalho CAM, Tirapelli DPC, Molina CAF, Tirapelli LF, Tucci S Abstract Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submit...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Association between type of feeding at discharge from the hospital and nutritional status of very low birth weight preterm infants.
Authors: Martins-Celini FP, Gonçalves-Ferri WA, Aragon DC, Bernichi JP, Calixto C, Sacramento EMF, Santos MA, Martinez FE Abstract The ideal feeding for premature babies has been the source of extensive debate. The aim of this study was to assess the association between type of feeding at discharge and the nutritional status of very low birth weight infants. This was a retrospective cohort of preterm babies with birth weight ≤1500 g, born between January 2006 and December 2013. The infants were divided into 3 groups according to type of feeding at discharge: exclusive breast milk (group 1), mixed feeding ...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Identification of a novel UMOD mutation (c.163G > A) in a Brazilian family with autosomal dominant tubulointerstitial kidney disease.
Identification of a novel UMOD mutation (c.163G>A) in a Brazilian family with autosomal dominant tubulointerstitial kidney disease. Braz J Med Biol Res. 2018 Mar 01;51(3):e6560 Authors: Lopes LB, Abreu CC, Souza CF, Guimaraes LER, Silva AA, Aguiar-Alves F, Kidd KO, Kmoch S, Bleyer AJ, Almeida JR Abstract Autosomal dominant tubulointerstitial kidney disease (ADTKD) is characterized by autosomal dominant inheritance, progressive chronic kidney disease, and a bland urinary sediment. ADTKD is most commonly caused by mutations in the UMOD gene encoding uromodulin (ADTKD-UMOD). We herein report the first ...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Low density lipoprotein modified silica nanoparticles loaded with docetaxel and thalidomide for effective chemotherapy of liver cancer.
Authors: Ao M, Xiao X, Ao Y Abstract In the present study, we successfully developed a docetaxel (DTX) and thalidomide (TDD) co-delivery system based on low density lipoprotein (LDL) modified silica nanoparticles (LDL/SLN/DTX/TDD). By employing the tumor homing property of LDL and the drug-loading capability of silica nanoparticles, the prepared LDL/SLN/DTX/TDD was expected to locate and specifically deliver the loaded drugs (DTX and TDD) to achieve effective chemotherapy of liver cancer. In vitro analysis revealed that nano-sized LDL/SLN/DTX/TDD with decent drug loading capabilities was able to increase the delive...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Lipid core nanoparticles resembling low-density lipoprotein and regression of atherosclerotic lesions: effects of particle size.
In conclusion, within the 20-100 nm range, size is apparently not an important feature regarding the LDE nanoparticle system and perhaps other solid lipid-based systems. PMID: 29513883 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effects of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) release from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone.
Authors: Ren Q, Cai M, Zhang K, Ren W, Su Z, Yang T, Sun T, Wang J Abstract Multiple growth factors can be administered to mimic the natural process of bone healing in bone tissue engineering. We investigated the effects of sequential release of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone regeneration. To improve the double emulsion/solvent evaporation technique, VEGF was encapsulated in PELA microcapsules, to which BMP-2 was attached. The scaffold (BMP-2/PELA/VEGF) was then fused t...
Source: Brazilian Journal of Medical and Biological Research - December 7, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3'-untranslated region.
Authors: Piranda DN, Abreu RBV, Freitas-Alves DR, de Carvalho MA, Vianna-Jorge R Abstract The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3'-untranslated region (3'-UTR). Different PR (rs689465, rs689466, rs20417) and 3'-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 a...
Source: Brazilian Journal of Medical and Biological Research - December 7, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Circulating levels of adiponectin and extent of coronary artery disease in patients undergoing elective coronary angiography.
Authors: Souza RA, Alves CMR, de Oliveira CSV, Reis AF, Carvalho AC Abstract Adiponectin (APN), an adipose tissue-released adipokine with demonstrated anti-inflammatory and anti-atherogenic properties, is encoded by a gene whose polymorphisms are associated with presence of coronary artery disease (CAD). Serum APN levels are inversely related with presence and complexity of CAD. Within this context, we sought to compare levels of total APN and its high molecular weight form (HMW APN) according to clinical presentation and extent of CAD in patients undergoing elective cardiac catheterization. From March 2008 to June...
Source: Brazilian Journal of Medical and Biological Research - December 7, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Ilex paraguariensis extracts extend the lifespan of Drosophila melanogaster fed a high-fat diet.
This study improves the understanding of natural interventions that reduce stress-induced oxidative damage, which is fundamental in promoting healthy ageing. PMID: 29211252 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - December 7, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Fasting: a major limitation for resistance exercise training effects in rodents.
Authors: das Neves W, de Oliveira LF, da Silva RP, Alves CRR, Lancha AH Abstract Protocols that mimic resistance exercise training (RET) in rodents present several limitations, one of them being the electrical stimulus, which is beyond the physiological context observed in humans. Recently, our group developed a conditioning system device that does not use electric shock to stimulate rats, but includes fasting periods before each RET session. The current study was designed to test whether cumulative fasting periods have some influence on skeletal muscle mass and function. Three sets of male Wistar rats were used in...
Source: Brazilian Journal of Medical and Biological Research - December 2, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Bevacizumab and gefitinib enhanced whole-brain radiation therapy for brain metastases due to non-small-cell lung cancer.
Authors: Yang RF, Yu B, Zhang RQ, Wang XH, Li C, Wang P, Zhang Y, Han B, Gao XX, Zhang L, Jiang ZM Abstract Non-small-cell lung cancer (NSCLC) patients who experience brain metastases are usually associated with poor prognostic outcomes. This retrospective study proposed to assess whether bevacizumab or gefitinib can be used to improve the effectiveness of whole brain radiotherapy (WBRT) in managing patients with brain metastases. A total of 218 NSCLC patients with multiple brain metastases were retrospectively included in this study and were randomly allocated to bevacizumab-gefitinib-WBRT group (n=76), gefitinib-...
Source: Brazilian Journal of Medical and Biological Research - December 2, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Short-term effects of stored homologous red blood cell transfusion on cardiorespiratory function and inflammation: an experimental study in a hypovolemia model.
Authors: Biagini S, Dale CS, Real JM, Moreira ES, Carvalho CRR, Schettino GPP, Wendel S, Azevedo LCP Abstract The pathophysiological mechanisms associated with the effects of red blood cell (RBC) transfusion on cardiopulmonary function and inflammation are unclear. We developed an experimental model of homologous 14-days stored RBC transfusion in hypovolemic swine to evaluate the short-term effects of transfusion on cardiopulmonary system and inflammation. Sixteen healthy male anesthetized swine (68±3.3 kg) were submitted to controlled hemorrhage (25% of blood volume). Two units of non-filtered RBC from each...
Source: Brazilian Journal of Medical and Biological Research - December 2, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Safety and efficacy of regional citrate anticoagulation in continuous blood purification treatment of patients with multiple organ dysfunction syndrome.
Authors: Tuerdi B, Zuo L, Sun H, Wang K, Wang Z, Li G Abstract The aim of this study was to discuss the safety and efficacy of regional citrate anticoagulation (RCA) on continuous blood purification (CBP) during the treatment of multiple organ dysfunction syndrome (MODS). Thirty-five patients with MODS were divided into two groups: the local citrate anticoagulation (RCA) group, and the heparin-free blood purification (hfBP) group. The MODS severity was assessed according to Marshall's MODS score criteria. Blood coagulation indicators, blood pressure, filter lifespan, filter replacement frequency, anticoagulation in...
Source: Brazilian Journal of Medical and Biological Research - December 2, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Decellularization of placentas: establishing a protocol.
This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for further analyses using histology, scanning electron microscopy, immunofluorescence and DNA quantification. Sodium dodecyl sulfate (SDS) was the most effective detergent for cell removal. Freezing placentas before decellularization required longer periods of incubation in different detergents. Both perfusion and ...
Source: Brazilian Journal of Medical and Biological Research - December 2, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Behavioral cross-sensitization between testosterone and fenproporex in adolescent and adult rats.
Authors: Conceição CQ, Engi SA, Cruz FC, Planeta CS Abstract The abuse of psychoactive drugs is considered a global health problem. During the last years, a relevant number of studies have investigated the relationship between anabolic-androgenic steroids (AAS) and other psychoactive drugs. AAS, such as testosterone, can cause a dependence syndrome that shares many features with the classical dependence to psychoactive substances. Pre-clinical evidence shows that there are interactions between testosterone and psychoactive drugs, such as cocaine. However, few studies have been performed to investigate...
Source: Brazilian Journal of Medical and Biological Research - December 2, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Relationship between aerobic capacity and pelvic floor muscles function: a cross-sectional study.
The objective of this study was to evaluate the relationship between aerobic capacity and pelvic floor muscles (PFM) function in adult women. Women aged 18 or over and without urinary dysfunction or other chronic diseases were eligible to participate. They completed the habitual physical activity (HPA) questionnaire, underwent a PFM functional evaluation by palpation and perineometry, and performed a submaximal (between 75 and 85% of maximum heart rate) cardiopulmonary exercise (CPX) test to determine the ventilatory anaerobic threshold (VAT). Forty-one women were included (35±16 years, 75% physically active, 17% ve...
Source: Brazilian Journal of Medical and Biological Research - September 28, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis.
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis...
Source: Brazilian Journal of Medical and Biological Research - September 28, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effects of herbal medicine Sijunzi decoction on rabbits after relieving intestinal obstruction.
This study aimed to evaluate the effects of Sijunzi decoction on restoring the immune function and nutritional status after relieving the obstruction. Experimental rabbits (2.5±0.2 kg) were randomly divided into normal control group, 2-day intestinal obstruction group, 2-day natural recovery group, 4-day natural recovery group, 2-day treated group, and 4-day treated group. Sijunzi decoction was given twice a day to the treated groups. The concentration of markers was analyzed to evaluate the immune function and nutritional status. The concentration of interleukin-2, immunoglobulins and complement components of the t...
Source: Brazilian Journal of Medical and Biological Research - September 28, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Absorption mechanism of three curcumin constituents through in situ intestinal perfusion method.
This study aimed to investigate the absorption mechanism of three curcumin constituents in rat small intestines. Self-emulsification was used to solubilize the three curcumin constituents, and the rat in situ intestinal perfusion method was used to study factors on drug absorption, including drug mass concentration, absorption site, and the different types and concentrations of absorption inhibitors. Within the scope of experimental concentrations, three curcumin constituents were absorbed in rat small intestines through the active transport mechanism. PMID: 28953988 [PubMed - in process] (Source: Brazilian Journal of ...
Source: Brazilian Journal of Medical and Biological Research - September 28, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Collaborative care model improves self-care ability, quality of life and cardiac function of patients with chronic heart failure.
This study aimed to explore the effects of collaborative care model (CCM) on patients with CHF. A total of 114 CHF patients were enrolled in this study, and were randomly and equally divided into two groups: control and experimental. Patients in the two groups received either usual care or CCM for 3 continuous months. The impacts of CCM on the self-care ability and quality of life were assessed using self-care of heart failure index and short form health survey 12, respectively. Further, cardiac function was assessed by measuring left ventricular ejection fraction (LVEF) and the level of N-terminal pro-B-type natriuretic p...
Source: Brazilian Journal of Medical and Biological Research - September 28, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Metabolomics as a promising tool for early osteoarthritis diagnosis.
Authors: de Sousa EB, Dos Santos GC, Duarte MEL, Moura V, Aguiar DP Abstract Osteoarthritis (OA) is the main cause of disability worldwide, due to progressive articular cartilage loss and degeneration. According to recent research, OA is more than just a degenerative disease due to some metabolic components associated to its pathogenesis. However, no biomarker has been identified to detect this disease at early stages or to track its development. Metabolomics is an emerging field and has the potential to detect many metabolites in a single spectrum using high resolution nuclear magnetic resonance (NMR) techniques o...
Source: Brazilian Journal of Medical and Biological Research - September 28, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research

Candesartan, rather than losartan, improves motor dysfunction in thioacetamide-induced chronic liver failure in rats.
In conclusion, therapeutic antifibrotic effects of losartan and candesartan in thioacetamide-induced hepatic fibrosis in rats are possibly through angiotensin-II receptor blocking, antioxidant, and anti-inflammatory activities. Improved motor dysfunction by candesartan could be attributed to better brain penetration and slower "off-rate" from angiotensin-II receptors. Clinical trials are recommended. PMID: 28953991 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - September 28, 2017 Category: Research Tags: Braz J Med Biol Res Source Type: research