Association between tumor necrosis factor polymorphisms and rheumatoid arthritis as well as systemic lupus erythematosus: a meta-analysis.
Authors: Chen L, Huang Z, Liao Y, Yang B, Zhang J Abstract Tumor necrosis factor-alpha (TNF-α) plays an important role in autoimmune diseases. Previous studies have investigated the association of TNF-α-238G/A (rs361525) and -308G/A (rs1800629) polymorphisms with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). However, no agreed conclusion had been made. Therefore, this meta-analysis was conducted to assess the associations of TNF-α-238G/A and -308G/A polymorphisms with RA and SLE risk. A systematic search was conducted in commonly used databases. Meta-analysis was performed by S...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Prognostic factors in patients undergoing early-start peritoneal dialysis within 24 h after catheter insertion.
This study aimed to investigate the clinical characteristics, prognosis, and factors for survival of patients who underwent early-start peritoneal dialysis (PD) within 24 h after catheter insertion three years after PD. This study was conducted from January 1, 2013 to December 31, 2017. All adult patients who were diagnosed with end-stage renal disease (ESRD) and underwent PD for the first time within 24 h after catheter insertion in our hospital were included. All patients with PD were followed-up until they withdrew from PD, switching to hemodialysis, were transferred to other medical centers, underwent renal transplanta...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method.
This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse blood with K. pneumoniae were used for sensitivity analysis. The detection limit of the LAMP was 1 bacterium/reaction. The results showed t...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effects of artepillin C on model membranes displaying liquid immiscibility.
Authors: Pazin WM, Vilanova N, Voets IK, Soares AEE, Ito AS Abstract It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and giant unilamelar vesicles) composed of ternary lipid mixtures containing cholesterol, which display liquid-ordered (lo) and liquid-disordered (ld) phase coexistence. Specifically, we explored po...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Burden, access, and disparities in kidney disease.
This article was published in Kidney International volume 95, pages 242-248, https://doi.org/10.1016/j.kint.2018.11.007, Copyright World Kidney Day 2019 Steering Committee (2019) and is reprinted concurrently in several journals. The articles cover identical concepts and wording, but vary in minor stylistic and spelling changes, detail, and length of manuscript in keeping with each journal's style. Any of these versions may be used in citing this article. Note that all authors contributed equally to the conception, preparation, and editing of the manuscript. Kidney disease is a global public health problem, affecting over ...
Source: Brazilian Journal of Medical and Biological Research - March 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Erratum for: "Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation" [Braz J Med Biol Res (2018) 51(10): e7439].
Erratum for: "Gastrodin induced HO-1 and Nrf2 up-regulation to alleviate H2O2-induced oxidative stress in mouse liver sinusoidal endothelial cells through p38 MAPK phosphorylation" [Braz J Med Biol Res (2018) 51(10): e7439]. Braz J Med Biol Res. 2019 02 25;52(2):e7439erratum Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20187439]. PMID: 30810603 [PubMed] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Observation of the effect of bone marrow mesenchymal stem cell transplantation by different interventions on cirrhotic rats.
Authors: Zhou X, Yang J, Liu Y, Li Z, Yu J, Wei W, Chen Q, Li C, Tang N Abstract Bone marrow mesenchymal stem cells (BMSCs) transplantation has attracted attention for the treatment of liver cirrhosis and end-stage liver diseases. Therefore, in this study, we evaluated the effect of different methods of BMSCs transplantation in the treatment of liver cirrhosis in rats. Seventy-two male Sprague-Dawley rats were divided into 7 groups: 10 were used to extract BMSCs, 10 were used as normal group, and the remaining 52 rats were randomly divided into five groups for testing: control group, BMSCs group, BMSCs+granulocyte ...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Protective effect of dexmedetomidine against organ dysfunction in a two-hit model of hemorrhage/resuscitation and endotoxemia in rats.
Authors: Jiang Y, Xia M, Huang Q, Ding D, Li Y, Zhang Z, Zhang X Abstract Dexmedetomidine (DEX), a selective agonist of α2-adrenergic receptors, has anti-inflammation properties and potential beneficial effects against trauma, shock, or infection. Therefore, this study aimed to investigate whether DEX might protect against multiple-organ dysfunction in a two-hit model of hemorrhage/resuscitation (HS) and subsequent endotoxemia. Eighty Wistar rats were randomized into four groups: NS (normal saline), HS/L (HS plus lipopolysaccharide), HS/L+D (HS/L plus dexmedetomidine), and HS/L+D+Y (HS/L+D plus yohimbine). Si...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Emodin protects H9c2 cells from hypoxia-induced injury by up-regulating miR-138 expression.
Authors: Zhang X, Qin Q, Dai H, Cai S, Zhou C, Guan J Abstract Myocardial infarction (MI) is a common presentation for ischemic heart disease, which is a leading cause of death. Emodin is a Chinese herbal anthraquinone used in several diseases. However, the effect of emodin in hypoxia-induced injury in cardiomyocytes has not been clearly elucidated. Our study aimed to clarify the functions of emodin in hypoxia-induced injury in rat cardiomyocytes H9c2 and explore the underlying mechanism. The effects of emodin on cell viability and apoptosis were analyzed by the Cell counting kit-8 assay and flow cytometry assay, r...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Visual and automatic classification of the cyclic alternating pattern in electroencephalography during sleep.
Authors: Largo R, Lopes MC, Spruyt K, Guilleminault C, Wang YP, Rosa AC Abstract Cyclic alternating pattern (CAP) is a neurophysiological pattern that can be visually scored by international criteria. The aim of this study was to verify the feasibility of visual CAP scoring using only one channel of sleep electroencephalogram (EEG) to evaluate the inter-scorer agreement in a variety of recordings, and to compare agreement between visual scoring and automatic scoring systems. Sixteen hours of single-channel European data format recordings from four different sleep laboratories with either C4-A1 or C3-A2 channels and...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effect of risperidone on proliferation and apoptosis of MC3T3-E1 cells.
Authors: Zheng L, Yang L, Zhao X, Long N, Li P, Wang Y Abstract This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprote...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Amifostine reduces inflammation and protects against 5-fluorouracil-induced oral mucositis and hyposalivation.
This study investigated the protective effect of amifostine on an experimental model of OM. Hamsters were divided into six groups: saline control group (5 mL/kg), mechanical trauma (scratches) of the right cheek pouch; 5-FU (60 and 40 mg/kg, ip, respectively, administered on days 1 and 2); amifostine (12.5, 25, or 50 mg/kg) + 5-FU + scratches. Salivation rate was assessed and the animals were euthanized on day 10 for the analysis of macroscopic and microscopic injury by scores. Tissue samples were harvested for the measurement of neutrophil infiltration and detection of inflammatory markers by ELISA and immunohistochemistr...
Source: Brazilian Journal of Medical and Biological Research - February 28, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Cirrhotic cardiomyopathy: the liver affects the heart.
Authors: Carvalho MVH, Kroll PC, Kroll RTM, Carvalho VN Abstract Cirrhotic cardiomyopathy historically has been confused as alcoholic cardiomyopathy. The key points for diagnosis of cirrhotic cardiomyopathy have been well explained, however this entity was neglected for a long time. Nowadays the diagnosis of this entity has become important because it is a factor that contributes significantly to morbidity-mortality in cirrhotic patients. Characteristics of cirrhotic cardiomyopathy are a hyperdynamic circulatory state, altered diastolic relaxation, impaired contractility, and electrophysiological abnormalities, par...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Kaempferol suppresses human gastric cancer SNU-216 cell proliferation, promotes cell autophagy, but has no influence on cell apoptosis.
This study aimed to investigate the effects of kaempferol on gastric cancer SNU-216 cell proliferation, apoptosis, and autophagy, as well as underlying potential mechanisms. Viability, proliferation, and apoptosis of SNU-216 cells after kaempferol treatment were evaluated using cell counting kit-8 assay, 5-btomo-2'-deoxyuridine incorporation assay, and annexin V-FITC/PI staining, respectively. Quantitative reverse transcription PCR was performed to measure the mRNA expressions of cyclin D1 and microRNA-181a (miR-181a) in SNU-216 cells. Cell transfection was used to down-regulate the expression of miR-181a. The protein expr...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Serotonin induces or inhibits neuritic regeneration of leech CNS neurons depending on neuronal identity.
Authors: Vargas J, Alfaro-Rodríguez A, Perez-Orive J Abstract Recovery of motor function after central nervous system (CNS) injury is dependent on the regeneration capacity of the nervous system, which is a multifactorial process influenced, among other things, by the role of neuromodulators such as serotonin. The neurotransmitter serotonin can promote neuronal regeneration but there are also reports of it causing restriction, so it is important to clarify these divergent findings in order to understand the direct scope and side effects of potential pharmacological treatments. We evaluated the effect of sero...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples.
Authors: Santos MFM, Oliveira FCAC, Kishimoto RK, Borri D, Santos FPS, Campregher PV, Silveira PAA, Hamerschlak N, Mangueira CLP, Duarte FB, Crepaldi AH, Salvino MA, Velloso EDRP Abstract Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndro...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications.
In conclusion, the VOB formulation contributed to the improvement of current therapeutic strategies for cutaneous applications in skin care. PMID: 30785481 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Thyroid disorders in Brazil: the contribution of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil).
Authors: Bensenor IM Abstract Thyroid disorders are common diseases, both in Brazil and worldwide. The Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) is a prospective cohort study that investigates cardiovascular diseases, diabetes, and associated factors, including non-classical cardiovascular risk factors such as thyroid function. Thyroid function was classified according to thyrotropin stimulating hormone (TSH), free thyroxine (FT4), and use of medication to treat thyroid disorders, after excluding participants who reported use of any medication that could alter the results of the TSH and FT4 tests. ...
Source: Brazilian Journal of Medical and Biological Research - February 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Erratum for: "Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II" [Braz J Med Biol Res (2018) 51(12): e7526].
Erratum for: "Participation of hepatic α/β-adrenoceptors and AT1 receptors in glucose release and portal hypertensive response induced by adrenaline or angiotensin II" [Braz J Med Biol Res (2018) 51(12): e7526]. Braz J Med Biol Res. 2019 Jan 10;52(2):e7526erratum Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20187526]. PMID: 30726313 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - February 8, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

CD47 expression is decreased in hematopoietic progenitor cells in patients with myelofibrosis.
Authors: Nonino A, Nascimento JM, Mascarenhas CC, Mazzeu JF, Pereira RW, Jacomo RH Abstract Myelofibrosis (MF) is characterized by increased circulating hematopoietic progenitor cells (HPCs), abnormal cytokine levels, and the survival advantage of neoplastic progenitors over their normal counterparts, which leads to progressive disappearance of polyclonal hematopoiesis. CD47 is a surface glycoprotein with many functions, such as acting as a phagocytosis inhibitor of the expressing cell, that is increased in normal hematopoietic stem and progenitor cells mobilized into the blood and several human cancer-initiating c...
Source: Brazilian Journal of Medical and Biological Research - December 13, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Voluntary wheel running: patterns and physiological effects in mice.
Authors: Manzanares G, Brito-da-Silva G, Gandra PG Abstract Exercise can prevent and improve the pathophysiology of diseases and promote healthy aging. Thus, understanding the mechanisms that regulate the beneficial effects of exercise may lead to the development of new strategies to enhance quality of life and to counteract chronic diseases. Voluntary wheel running is an interesting model to study the effects of exercise in mice. Compared to forced treadmill exercise, voluntary wheel running presents several advantages such as: 1) running pattern is similar to natural running behavior of mice; 2) it is performed u...
Source: Brazilian Journal of Medical and Biological Research - December 13, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Kinetics of acid-base parameters in conventional hemodialysis.
Authors: Lugon JR, Pereira GRM, Strogoff-de-Matos JP, Peixoto AJ Abstract Details about the acid-base changes in hemodialysis are scarce in the literature but are potentially relevant to adequate management of patients. We addressed the acid-base kinetics during hemodialysis and throughout the interdialytic period in a cross-sectional study of adults undergoing conventional hemodialysis. Samples for blood gas analysis were obtained from the arterial limb of the arteriovenous fistula before the first session of the week (HD1), immediately at the end of HD1, and on sequential collections at 15, 30, 45, 60, and 120 mi...
Source: Brazilian Journal of Medical and Biological Research - December 13, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Adipose tissue is less responsive to food restriction anti-inflammatory effects than liver, muscle, and brain in mice.
Authors: Antunes MM, de Almeida-Souza CB, Godoy G, Crisma AR, Masi LN, Curi R, Bazotte RB Abstract High caloric intake promotes chronic inflammation, insulin resistance, and chronic diseases such as type-2 diabetes, which may be prevented by food restriction (FR). The effect of FR on expression of pro-inflammatory and anti-inflammatory genes in adipose tissue, liver, muscle, and brain was compared. Male Swiss mice were submitted to FR (FR group) or had free access to food (control group) during 56 days. The liver, gastrocnemius muscle, brain, and epididymal white adipose tissue (WAT) were collected for analysis of ...
Source: Brazilian Journal of Medical and Biological Research - December 13, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Analysis of the application values of different combination schemes of liquid-based cytology and high-risk human papilloma virus test in the screening of high-grade cervical lesions.
Authors: Wang J Abstract The aim of this study was to explore the value of different combination schemes of liquid-based cytology (LBC) and high-risk human papilloma virus (HPV) test in the screening of high-grade (≥CIN 2) cervical lesions. From 5727 women who had undergone examinations with LBC and high-risk HPV test, 1884 patients with positive results of either or both LBC and HPV test were included in this study and underwent cervical biopsy. Based on the pathological examination results, comparisons of the assessment indicators of all diagnostic tests were made, and the application values of LBC and high-ri...
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/ β-catenin pathway.
In conclusion, we demonstrated that GPNMB contributed to the tumorigenesis of cervical cancer, at least in part, by regulating MMP-2/MMP-9 activity in tumor cells via activation of canonical Wnt/β-catenin signaling. This might be a potential therapeutic target for treating human cervical cancer. PMID: 30484490 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression.
This study aimed to explore the function of HtrA1 in pancreatic cancer cell growth and its underlying mechanism. We found that the expression of HtrA1 was lower in pancreatic cancer tissue compared to the adjacent normal tissue. Consistently, HtrA1 levels were also decreased in two human pancreatic cancer cell lines, PANC-1 and BXPC-3. Moreover, enforced expression of HtrA1 inhibited cell viability and colony formation of PANC-1 and BXPC-3 cells. Overexpression of HtrA1 promoted apoptosis and suppressed migratory ability of tumor cells. On the contrary, siRNA-mediated knockdown of HtrA1 promoted the growth potential of pan...
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

FGFR1 is an independent prognostic factor and can be regulated by miR-497 in gastric cancer progression.
Authors: Xie G, Ke Q, Ji YZ, Wang AQ, Jing M, Zou LL Abstract Fibroblast growth factor receptor 1 (FGFR1) has been reported in gastric cancer to be a prognostic factor. However, miR-497-targeted FGFR1 has not been explored in the carcinogenesis of gastric cancer. The present study intended to revalidate the prognostic significance of FGFR1 in patients with gastric cancer, and the mechanism of miR-497-regulated FGFR1 was investigated in gastric cancer cell proliferation and apoptosis. The messenger RNA (mRNA) and protein levels were assayed by RT-qPCR and western blotting, respectively. The targeted genes were predi...
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Necroptosis occurs in osteoblasts during tumor necrosis factor- α stimulation and caspase-8 inhibition.
In conclusion, TNF-α induced preferentially apoptosis in osteoblast cell line and necroptosis played a decisive role when TNF-α-induced death was inhibited by the inhibitor of apoptosis. Combined treatment with Nec-1 and Z-IETD-FMK protected mouse osteoblasts from death induced by TNF-α. PMID: 30484493 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy.
Authors: Jin B, Zhu J, Shi HM, Wen ZC, Wu BW Abstract Yes-associated protein (YAP) is an important regulator of cellular proliferation and transdifferentiation. However, little is known about the mechanisms underlying myofibroblast transdifferentiation in dilated cardiomyopathy (DCM). We investigated the role of YAP in the pathological process of cardiac matrix remodeling. A classic model of DCM was established in BALB/c mice by immunization with porcine cardiac myosin. Cardiac fibroblasts were isolated from neonatal Sprague-Dawley rats by density gradient centrifugation. The expression levels of α-smooth mus...
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/ β-catenin signaling pathway.
In this study, we observed that malignant melanoma tissue showed NOP14 down-regulation compared to melanocytic nevi tissues. Moreover, we observed that NOP14 expression was significantly associated with melanoma tumor thickness and lymph node metastasis. NOP14 overexpression in melanoma cells suppressed proliferation, caused G1 phase arrest, promoted apoptosis, and inhibited melanoma cell migration and invasion. Further investigations revealed that NOP14 overexpression reduced the expression levels of Wnt3a, β-catenin, and GSK-3β of the Wnt/β-catenin pathway. In summary, we demonstrated that NOP14 inhibited ...
Source: Brazilian Journal of Medical and Biological Research - November 30, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Insights on the epigenetic mechanisms underlying pulmonary arterial hypertension.
Authors: Luna RCP, de Oliveira Y, Lisboa JVC, Chaves TR, de Araújo TAM, de Sousa EE, Miranda Neto M, Pirola L, Braga VA, de Brito Alves JL Abstract Pulmonary arterial hypertension (PAH), characterized by localized increased arterial blood pressure in the lungs, is a slow developing long-term disease that can be fatal. PAH is characterized by inflammation, vascular tone imbalance, pathological pulmonary vascular remodeling, and right-sided heart failure. Current treatments for PAH are palliative and development of new therapies is necessary. Recent and relevant studies have demonstrated that epigenetic proces...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Cat ownership is associated with increased asthma prevalence and dog ownership with decreased spirometry values.
Authors: Simoneti CS, Ferraz E, Menezes MB, Icuma TR, Vianna EO Abstract The association between pet ownership and the development of allergic and respiratory diseases has been the aim of several studies, however, the effects of exposure in adults remain uncertain. The aims of the present study were to investigate the prevalence of asthma and lung function status among dog and cat owners. This cross-sectional study was performed at two universities with students and workers who were allocated into 3 groups according to pet ownership in the previous year: cat owners, dog owners, and no pets (control group). Subjects...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts.
In this study, we explored the effects and mechanism of miR-221 on the growth and migration of osteoblasts using MC3T3-E1 cells. The expression levels of miR-221 in the different groups were measured by qRT-PCR. Then, miR-221 mimic and inhibitor were transfected into MC3T3-E1 cells, and cell viability and migration were measured using the CCK-8 assay and the Transwell migration assay. Additionally, the expression levels of differentiation-related factors (Runx2 and Ocn) and ZFPM2 were measured by qRT-PCR. Western blot was used to measure the expression of cell cycle-related proteins, epithelial-mesenchymal transition (EMT)...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways.
This study aimed to investigate the effects of LIPUS on proliferation and migration of HaCaT cells as well as the regulatory mechanisms associated with signaling pathways. Human HaCaT cells were exposed or not to LIPUS, and cell proliferation and migration were measured by BrdU incorporation assay and Transwell assay, respectively. Expression of proteins associated with proliferation and migration was evaluated by western blot analysis. Expression of key kinases in the PI3K/AKT and JNK pathways was also evaluated by western blot analysis. Effects of LIPUS on the PI3K/AKT and JNK pathways, and whether LIPUS affected HaCaT c...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway.
Authors: Zheng G, Qu H, Li F, Ma W, Yang H Abstract Previous studies have indicated that propofol has immunomodulatory and antioxidative properties. However, the renoprotection effect and the precise mechanisms of propofol in sepsis-induced renal injury remain unclear. The purpose of the present study was to investigate the role of miR-290-5p/CCL-2 signaling in septic mice treatment with propofol. Mice were treated with propofol (50 mg/kg) twice within 24 h. Survival outcome was monitored within 48 h. The mRNA and protein levels were assayed by qRT-PCR and western blotting, respectively. Mouse podocytes (MPC5) were...
Source: Brazilian Journal of Medical and Biological Research - October 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia.
This study was designed to provide laboratory evidence supporting the hematopoietic effect of Beta vulgaris (beet) leaf aqueous extract in phenylhydrazine-induced anemia model in albino rats. Extraction of the leaves/stalks was done by maceration in 30% hydro-ethanol for 48 h. An intraperitoneal injection of 20 mg/kg phenylhydrazine was applied for two consecutive days to develop hemolytic anemia on the 4th day after the 1st injection in 24 of 30 male albino rats. The animals were divided into 5 groups and received the following treatments: standard (ferrous ascorbate + folic acid; 13.5 + 0.135 mg/kg), B. vulgaris extract ...
Source: Brazilian Journal of Medical and Biological Research - October 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Cardiorespiratory and metabolic determinants during moderate and high resistance exercise intensities until exhaustion using dynamic leg press: comparison with critical load.
The objective of this study was to assess cardiovascular, respiratory, and metabolic responses during a commonly used dynamic leg press resistance exercise until exhaustion (TEx) at different intensities and compare with critical load (CL). This was a prospective, cross-sectional, controlled, and crossover study. Twelve healthy young men (23±2.5 years old) participated. The subjects carried out three bouts of resistance exercise in different percentages of 1 repetition maximum (60, 75, and 90% 1RM) until TEx. CL was obtained by means of hyperbolic model and linearization of the load-duration function. During all bou...
Source: Brazilian Journal of Medical and Biological Research - October 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Icariin reduces human colon carcinoma cell growth and metastasis by enhancing p53 activities.
Authors: Tian M, Yang S, Yan X Abstract Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay, and Annexin V-FITC/PI double staining method were carried out to determine the changes of HCT116 cell growth and migration. mRNA and protein expressions were determined by quantitative real-time PCR and western blot, resp...
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF- κB and MAPK pathways.
In conclusion, BAI had anti-inflammatory and anti-oxidative effects on TIN patients and LPS-induced RPTECs by down-regulating NF-κB and MAPK pathways. PMID: 30088539 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Adrenergic receptor β3 is involved in the memory consolidation process in mice.
Adrenergic receptor β3 is involved in the memory consolidation process in mice. Braz J Med Biol Res. 2018 Aug 06;51(10):e7564 Authors: Souza-Braga P, Lorena FB, Nascimento BPP, Marcelino CP, Ravache TT, Ricci E, Bernardi MM, Ribeiro MO Abstract Attention and emotion have a positive impact on memory formation, which is related to the activation of the noradrenergic system in the brain. The hippocampus and amygdala are fundamental structures in memory acquisition, which is modulated by noradrenaline through the noradrenergic receptors. Pharmacological studies suggest that memory acquisition depends ...
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Saquinavir plus methylprednisolone ameliorates experimental acute lung injury.
This study demonstrated that SQV prevented experimental ALI and improved glucocorticoid insensitivity by modulating the HMGB1/TLR4 pathway. PMID: 30088541 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Transplantation of rat-derived microglial cells promotes functional recovery in a rat model of spinal cord injury.
This study evaluated the effect of microglia transplantation on neurological functional recovery in rats subjected to traumatic spinal cord injury (SCI). The rat model of SCI was established using a weight drop device. Forty SCI rats were randomly divided into the microglia group and the saline group. Then, rat-derived microglial cells or normal saline was injected into the injured site 7 days after surgery. The Basso-Beattie-Bresnahan (BBB) score, inclined plate test, and motor-evoked potentials (MEPs) were applied to assess the recovery of motor function. Hematoxylin and eosin (H&E) staining was used to assess the th...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Omphaloceles in combination with a self-made hemispherical cushion: a report of 12 cases.
Authors: Huang G, Ma L, Wu Y Abstract The aim of this study was to discuss the curative effect of applying "capsule-reserved normal saline bag and self-made hemi-spherical cushion oppression" for treating giant omphaloceles. Twelve patients with giant omphaloceles who were admitted to our hospital between January 2008 and June 2016 were selected for treatment as follows: a capsule-reserved normal saline bag was used to promote the gradual return of the abdominal contents into the abdominal cavity in phase I, and a self-made hemi-spherical cushion was used for compression combined with a local dressing cha...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Comparison of endoplasmic reticulum stress and mitochondrial biogenesis responses after 12 weeks of treadmill running and ladder climbing exercises in the cardiac muscle of middle-aged obese rats.
Authors: Kim K, Ahn N, Jung S Abstract The purpose of the present study was to compare the influence of aerobic exercise (AE) lasting 12 weeks to that of resistance exercise (RE) of the same duration on endoplasmic reticulum (ER) stress and mitochondrial biogenesis in the cardiac muscle of middle-aged obese rats. Obesity was induced in thirty 50-week-old male Sprague Dawley rats over 6 weeks by administration of a high-fat diet. The rats were then subjected to treadmill-running (AE) and ladder-climbing (RE) exercises 3 times per week for 12 weeks. Rats in the AE group showed significantly lower increases in body we...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Transcriptomic differential lncRNA expression is involved in neuropathic pain in rat dorsal root ganglion after spared sciatic nerve injury.
Authors: Mao P, Li CR, Zhang SZ, Zhang Y, Liu BT, Fan BF Abstract Dorsal root ganglia (DRG) neurons regenerate spontaneously after traumatic or surgical injury. Long noncoding RNAs (lncRNAs) are involved in various biological regulation processes. Conditions of lncRNAs in DRG neuron injury deserve to be further investigated. Transcriptomic analysis was performed by high-throughput Illumina HiSeq2500 sequencing to profile the differential genes in L4-L6 DRGs following rat sciatic nerve tying. A total of 1,228 genes were up-regulated and 1,415 down-regulated. By comparing to rat lncRNA database, 86 known and 26 novel...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains.
This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis a...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Up-regulated expression of CD147 gene in malignant bone tumor and the possible induction mechanism during osteoclast formation.
In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h, which suggested that RANKL could promote the formation of osteoclast, indicating that both CD147 and RANKL play important roles in the formation of osteoclasts. Furthermore, the expressions of four osteoclast specific expression genes, including TRACP, ...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Plasma lipid abnormalities in Pakistani population: trends, associated factors, and clinical implications.
Authors: Zaid M, Hasnain S Abstract Previous studies have reported increased prevalence of coronary heart disease (CHD) in Indians and South Asian settlers in North America. This increased burden of CHD among South Asians is mainly caused by dyslipidemia. To the best of our knowledge, none of the previous works has studied the patterns and prevalence of dyslipidemia in the Pakistani population. The present work aimed to study the plasma lipid trends and abnormalities in a population-based sample of urban and rural Pakistanis. The study included 238 participants (108 males,130 females). Plasma lipid profiles of the ...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Decreased muscle strength is associated with proinflammatory cytokines but not testosterone levels in men with diabetes.
Authors: Ferreira JP, Leal AMO, Vasilceac FA, Sartor CD, Sacco ICN, Soares AS, Salvini TF Abstract The aim of this study was to compare muscle strength in male subjects with type 2 diabetes mellitus (DM2) with and without low plasma testosterone levels and assess the relationship between muscle strength, testosterone levels, and proinflammatory cytokines. Males (75) aged between 18 and 65 years were divided into 3 groups: control group that did not have diabetes and had a normal testosterone plasma level (>250 ng/dL), DnormalTT group that had DM2 with normal testosterone levels, and the DlowTT group that had DM2...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis.
Authors: Lu Y, Zhang R, Wang Z, Zhou S, Song Y, Chen L, Chen N, Liu W, Ji C, Wu W, Zhang L Abstract We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses were used to determine the levels of cell proliferation and apoptosis. Western blot analyses were performed to analyze the relevant clinical indicators of hidrotic ectodermal dysplasia and markers of apoptosis in transfec...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research