Insights on the epigenetic mechanisms underlying pulmonary arterial hypertension.
Authors: Luna RCP, de Oliveira Y, Lisboa JVC, Chaves TR, de Araújo TAM, de Sousa EE, Miranda Neto M, Pirola L, Braga VA, de Brito Alves JL Abstract Pulmonary arterial hypertension (PAH), characterized by localized increased arterial blood pressure in the lungs, is a slow developing long-term disease that can be fatal. PAH is characterized by inflammation, vascular tone imbalance, pathological pulmonary vascular remodeling, and right-sided heart failure. Current treatments for PAH are palliative and development of new therapies is necessary. Recent and relevant studies have demonstrated that epigenetic proces...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Cat ownership is associated with increased asthma prevalence and dog ownership with decreased spirometry values.
Authors: Simoneti CS, Ferraz E, Menezes MB, Icuma TR, Vianna EO Abstract The association between pet ownership and the development of allergic and respiratory diseases has been the aim of several studies, however, the effects of exposure in adults remain uncertain. The aims of the present study were to investigate the prevalence of asthma and lung function status among dog and cat owners. This cross-sectional study was performed at two universities with students and workers who were allocated into 3 groups according to pet ownership in the previous year: cat owners, dog owners, and no pets (control group). Subjects...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts.
In this study, we explored the effects and mechanism of miR-221 on the growth and migration of osteoblasts using MC3T3-E1 cells. The expression levels of miR-221 in the different groups were measured by qRT-PCR. Then, miR-221 mimic and inhibitor were transfected into MC3T3-E1 cells, and cell viability and migration were measured using the CCK-8 assay and the Transwell migration assay. Additionally, the expression levels of differentiation-related factors (Runx2 and Ocn) and ZFPM2 were measured by qRT-PCR. Western blot was used to measure the expression of cell cycle-related proteins, epithelial-mesenchymal transition (EMT)...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways.
This study aimed to investigate the effects of LIPUS on proliferation and migration of HaCaT cells as well as the regulatory mechanisms associated with signaling pathways. Human HaCaT cells were exposed or not to LIPUS, and cell proliferation and migration were measured by BrdU incorporation assay and Transwell assay, respectively. Expression of proteins associated with proliferation and migration was evaluated by western blot analysis. Expression of key kinases in the PI3K/AKT and JNK pathways was also evaluated by western blot analysis. Effects of LIPUS on the PI3K/AKT and JNK pathways, and whether LIPUS affected HaCaT c...
Source: Brazilian Journal of Medical and Biological Research - October 27, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway.
Authors: Zheng G, Qu H, Li F, Ma W, Yang H Abstract Previous studies have indicated that propofol has immunomodulatory and antioxidative properties. However, the renoprotection effect and the precise mechanisms of propofol in sepsis-induced renal injury remain unclear. The purpose of the present study was to investigate the role of miR-290-5p/CCL-2 signaling in septic mice treatment with propofol. Mice were treated with propofol (50 mg/kg) twice within 24 h. Survival outcome was monitored within 48 h. The mRNA and protein levels were assayed by qRT-PCR and western blotting, respectively. Mouse podocytes (MPC5) were...
Source: Brazilian Journal of Medical and Biological Research - October 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia.
This study was designed to provide laboratory evidence supporting the hematopoietic effect of Beta vulgaris (beet) leaf aqueous extract in phenylhydrazine-induced anemia model in albino rats. Extraction of the leaves/stalks was done by maceration in 30% hydro-ethanol for 48 h. An intraperitoneal injection of 20 mg/kg phenylhydrazine was applied for two consecutive days to develop hemolytic anemia on the 4th day after the 1st injection in 24 of 30 male albino rats. The animals were divided into 5 groups and received the following treatments: standard (ferrous ascorbate + folic acid; 13.5 + 0.135 mg/kg), B. vulgaris extract ...
Source: Brazilian Journal of Medical and Biological Research - October 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Cardiorespiratory and metabolic determinants during moderate and high resistance exercise intensities until exhaustion using dynamic leg press: comparison with critical load.
The objective of this study was to assess cardiovascular, respiratory, and metabolic responses during a commonly used dynamic leg press resistance exercise until exhaustion (TEx) at different intensities and compare with critical load (CL). This was a prospective, cross-sectional, controlled, and crossover study. Twelve healthy young men (23±2.5 years old) participated. The subjects carried out three bouts of resistance exercise in different percentages of 1 repetition maximum (60, 75, and 90% 1RM) until TEx. CL was obtained by means of hyperbolic model and linearization of the load-duration function. During all bou...
Source: Brazilian Journal of Medical and Biological Research - October 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Icariin reduces human colon carcinoma cell growth and metastasis by enhancing p53 activities.
Authors: Tian M, Yang S, Yan X Abstract Icariin has been reported to possess high anticancer activity. Colon carcinoma is one of the leading causes of cancer-related mortality worldwide. Here, the anticancer activity of icariin against HCT116 colon carcinoma cells and the possible underlying mechanism were studied. The trypan blue staining assay, wound healing assay, clonogenic assay, CCK-8 assay, and Annexin V-FITC/PI double staining method were carried out to determine the changes of HCT116 cell growth and migration. mRNA and protein expressions were determined by quantitative real-time PCR and western blot, resp...
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF- κB and MAPK pathways.
In conclusion, BAI had anti-inflammatory and anti-oxidative effects on TIN patients and LPS-induced RPTECs by down-regulating NF-κB and MAPK pathways. PMID: 30088539 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Adrenergic receptor β3 is involved in the memory consolidation process in mice.
Adrenergic receptor β3 is involved in the memory consolidation process in mice. Braz J Med Biol Res. 2018 Aug 06;51(10):e7564 Authors: Souza-Braga P, Lorena FB, Nascimento BPP, Marcelino CP, Ravache TT, Ricci E, Bernardi MM, Ribeiro MO Abstract Attention and emotion have a positive impact on memory formation, which is related to the activation of the noradrenergic system in the brain. The hippocampus and amygdala are fundamental structures in memory acquisition, which is modulated by noradrenaline through the noradrenergic receptors. Pharmacological studies suggest that memory acquisition depends ...
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Saquinavir plus methylprednisolone ameliorates experimental acute lung injury.
This study demonstrated that SQV prevented experimental ALI and improved glucocorticoid insensitivity by modulating the HMGB1/TLR4 pathway. PMID: 30088541 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - August 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Transplantation of rat-derived microglial cells promotes functional recovery in a rat model of spinal cord injury.
This study evaluated the effect of microglia transplantation on neurological functional recovery in rats subjected to traumatic spinal cord injury (SCI). The rat model of SCI was established using a weight drop device. Forty SCI rats were randomly divided into the microglia group and the saline group. Then, rat-derived microglial cells or normal saline was injected into the injured site 7 days after surgery. The Basso-Beattie-Bresnahan (BBB) score, inclined plate test, and motor-evoked potentials (MEPs) were applied to assess the recovery of motor function. Hematoxylin and eosin (H&E) staining was used to assess the th...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Omphaloceles in combination with a self-made hemispherical cushion: a report of 12 cases.
Authors: Huang G, Ma L, Wu Y Abstract The aim of this study was to discuss the curative effect of applying "capsule-reserved normal saline bag and self-made hemi-spherical cushion oppression" for treating giant omphaloceles. Twelve patients with giant omphaloceles who were admitted to our hospital between January 2008 and June 2016 were selected for treatment as follows: a capsule-reserved normal saline bag was used to promote the gradual return of the abdominal contents into the abdominal cavity in phase I, and a self-made hemi-spherical cushion was used for compression combined with a local dressing cha...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Comparison of endoplasmic reticulum stress and mitochondrial biogenesis responses after 12 weeks of treadmill running and ladder climbing exercises in the cardiac muscle of middle-aged obese rats.
Authors: Kim K, Ahn N, Jung S Abstract The purpose of the present study was to compare the influence of aerobic exercise (AE) lasting 12 weeks to that of resistance exercise (RE) of the same duration on endoplasmic reticulum (ER) stress and mitochondrial biogenesis in the cardiac muscle of middle-aged obese rats. Obesity was induced in thirty 50-week-old male Sprague Dawley rats over 6 weeks by administration of a high-fat diet. The rats were then subjected to treadmill-running (AE) and ladder-climbing (RE) exercises 3 times per week for 12 weeks. Rats in the AE group showed significantly lower increases in body we...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Transcriptomic differential lncRNA expression is involved in neuropathic pain in rat dorsal root ganglion after spared sciatic nerve injury.
Authors: Mao P, Li CR, Zhang SZ, Zhang Y, Liu BT, Fan BF Abstract Dorsal root ganglia (DRG) neurons regenerate spontaneously after traumatic or surgical injury. Long noncoding RNAs (lncRNAs) are involved in various biological regulation processes. Conditions of lncRNAs in DRG neuron injury deserve to be further investigated. Transcriptomic analysis was performed by high-throughput Illumina HiSeq2500 sequencing to profile the differential genes in L4-L6 DRGs following rat sciatic nerve tying. A total of 1,228 genes were up-regulated and 1,415 down-regulated. By comparing to rat lncRNA database, 86 known and 26 novel...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Intestinal cell migration damage induced by enteropathogenic Escherichia coli strains.
This study aimed to investigate the effects of EPEC infection on intestinal epithelial cell migration in vitro, as well as the involvement of type III secretion system (T3SS) and Rho GTPases. Crypt intestinal epithelial cells (IEC-6) were infected with EPEC strains (E2348/69, ΔescF, and the LDI001 strain isolated from a malnourished Brazilian child) and commensal E. coli HS. Wound migration and cell death assays were performed at different time-points. Transcription and expression of Rho GTPases were evaluated using real-time PCR and western blotting. Overall, EPEC E2348/69 reduced migration and increased apoptosis a...
Source: Brazilian Journal of Medical and Biological Research - August 3, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Up-regulated expression of CD147 gene in malignant bone tumor and the possible induction mechanism during osteoclast formation.
In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h, which suggested that RANKL could promote the formation of osteoclast, indicating that both CD147 and RANKL play important roles in the formation of osteoclasts. Furthermore, the expressions of four osteoclast specific expression genes, including TRACP, ...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Plasma lipid abnormalities in Pakistani population: trends, associated factors, and clinical implications.
Authors: Zaid M, Hasnain S Abstract Previous studies have reported increased prevalence of coronary heart disease (CHD) in Indians and South Asian settlers in North America. This increased burden of CHD among South Asians is mainly caused by dyslipidemia. To the best of our knowledge, none of the previous works has studied the patterns and prevalence of dyslipidemia in the Pakistani population. The present work aimed to study the plasma lipid trends and abnormalities in a population-based sample of urban and rural Pakistanis. The study included 238 participants (108 males,130 females). Plasma lipid profiles of the ...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Decreased muscle strength is associated with proinflammatory cytokines but not testosterone levels in men with diabetes.
Authors: Ferreira JP, Leal AMO, Vasilceac FA, Sartor CD, Sacco ICN, Soares AS, Salvini TF Abstract The aim of this study was to compare muscle strength in male subjects with type 2 diabetes mellitus (DM2) with and without low plasma testosterone levels and assess the relationship between muscle strength, testosterone levels, and proinflammatory cytokines. Males (75) aged between 18 and 65 years were divided into 3 groups: control group that did not have diabetes and had a normal testosterone plasma level (>250 ng/dL), DnormalTT group that had DM2 with normal testosterone levels, and the DlowTT group that had DM2...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Mechanistic effect of the human GJB6 gene and its mutations in HaCaT cell proliferation and apoptosis.
Authors: Lu Y, Zhang R, Wang Z, Zhou S, Song Y, Chen L, Chen N, Liu W, Ji C, Wu W, Zhang L Abstract We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses were used to determine the levels of cell proliferation and apoptosis. Western blot analyses were performed to analyze the relevant clinical indicators of hidrotic ectodermal dysplasia and markers of apoptosis in transfec...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer.
In this study, we assessed the prognostic value of CHD 9 in patients with colorectal cancer (CRC). We screened for CHD 9 expression using immunohistochemical analysis in 87 surgical CRC specimens and found that the expression was upregulated in 81.5% of the cases, while 7.4% were decreased; in the remaining 11.1% of the cases, levels were not altered. Kaplan-Meier analysis showed that patients with high CHD 9 expression had better prognosis than those with low CHD 9 expression (54.5 vs 32.1%, P=0.034). Subsequently, Cox multi-factor survival regression analysis revealed that expression of CHD 9 protein was an independent p...
Source: Brazilian Journal of Medical and Biological Research - July 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin.
Authors: Bracesco N, Sosa V, Blanc L, Contreras V, Candreva EC, Salvo VA, Hocart S, Mechoso B, Nunes E Abstract DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. E...
Source: Brazilian Journal of Medical and Biological Research - July 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

NLRP3 inflammasome signaling as an early molecular response is negatively controlled by miR-186 in CFA-induced prosopalgia mice.
In this study, a post-translational mechanism of microRNA (miR)-186 via regulating the expression of NLRP3 in the complete Freund's adjuvant (CFA)-treated mice was investigated. The injection of CFA was used to induce trigeminal neuropathic pain in mice. miRs microarray chip assay was performed in trigeminal ganglions (TGs). CFA treatment significantly increased the mRNA expression of NLRP3, interleukin (IL)-1β, and IL-18 in TGs compared to the control group. Moreover, 26 miRs were differentially expressed in TGs from trigeminal neuropathic pain mice, and the expression of miR-186 showed the lowest level of all the mi...
Source: Brazilian Journal of Medical and Biological Research - July 19, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Imbalance of γδT17/γδTreg cells in the pathogenesis of allergic asthma induced by ovalbumin.
Imbalance of γδT17/γδTreg cells in the pathogenesis of allergic asthma induced by ovalbumin. Braz J Med Biol Res. 2018 Jul 10;51(9):e7127 Authors: Yang X, Zhang JH, Deng WS, Li CQ Abstract We aimed to explore the imbalance between the T helper 17 γδT cells (γδT17) and the regulatory γδT cells (γδTreg) in asthmatic mice. Male Balb/c mice were randomly divided into the normal control group and the asthmatic model group. The asthmatic model group mice were intraperitoneally injected with the mixture of ovalbumin (OVA)/Al(OH)3 and then...
Source: Brazilian Journal of Medical and Biological Research - July 12, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Strontium ranelate inhibits wear particle-induced aseptic loosening in mice.
Authors: Geng T, Sun S, Yu H, Guo H, Zheng M, Zhang S, Chen X, Jin Q Abstract The imbalance between bone formation and osteolysis plays a key role in the pathogenesis of aseptic loosening. Strontium ranelate (SR) can promote bone formation and inhibit osteolysis. The aim of this study was to explore the role and mechanism of SR in aseptic loosening induced by wear particles. Twenty wild-type (WT) female C57BL/6j mice and 20 sclerostin-/- female C57BL/6j mice were used in this study. Mice were randomly divided into four groups: WT control group, WT SR group, knockout (KO) control group, and KO SR group. We found tha...
Source: Brazilian Journal of Medical and Biological Research - July 12, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Mechanisms involved in anti-aging effects of guarana (Paullinia cupana) in Caenorhabditis elegans.
This study demonstrated that GEE acts through antioxidant activity, DAF-16, HSF-1, and SKN-1 pathways, and human adenosine receptor ortholog (ADOR-1) to extend lifespan. GEE also downregulated skn-1, daf-16, sir-2.1 and hsp-16.2 in 9-day-old C. elegans, which might reflect less need to activate these protective genes due to direct antioxidant effects. Our results contribute to the comprehension of guarana effects in vivo, which might be helpful to prevent or treat aging-associated disorders, and also suggest purinergic signaling as a plausible therapeutic target for longevity studies. PMID: 29972429 [PubMed - in proces...
Source: Brazilian Journal of Medical and Biological Research - July 5, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Establishment of a rat model for uterine leiomyomas based on Western and traditional Chinese medicine theories.
In this study, for the first time, traditional Chinese medicine and Western medicine were combined to establish an animal model of ULs in rats. In order to evaluate the function and value of the novel model, it was compared with other models. The long-term and short-term rat models for ULs were established using progesterone and diethylstilbestrol. Rats in Qi stagnation and blood stasis group were injected with epinephrine hydrochloride and received chronic unpredictable stress for two weeks. Rats in combining disease with syndrome group (CDWSG) received not only epinephrine hydrochloride injection and chronic unpredictabl...
Source: Brazilian Journal of Medical and Biological Research - July 5, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2.2.15 cells by targeting Lin28a/let-7a axis.
Authors: Qiu Z, Zhou J, Zhang C, Cheng Y, Hu J, Zheng G Abstract An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induce...
Source: Brazilian Journal of Medical and Biological Research - May 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Efficacy, acceptability, and tolerability of antidepressant treatments for patients with post-stroke depression: a network meta-analysis.
Authors: Qin B, Chen H, Gao W, Zhao LB, Zhao MJ, Qin HX, Chen W, Chen L, Yang MX Abstract The aim of this study was to investigate the efficacy, acceptability, and tolerability of antidepressants in treating post-stroke depression (PSD) by performing a network meta-analysis of randomized controlled trials of the current literature. Eligible studies were retrieved from online databases, and relevant data were extracted. The primary outcome was efficacy as measured by the mean change in overall depressive symptoms. Secondary outcomes included discontinued treatment for any reason and specifically due to adverse event...
Source: Brazilian Journal of Medical and Biological Research - May 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Epoetin alfa resistance in hemodialysis patients with chronic kidney disease: a longitudinal study.
This study aimed to investigate the factors associated with resistance to recombinant human erythropoietin therapy with epoetin alfa (αEPO). We performed a prospective, longitudinal study of hemodialysis patients receiving treatment with αEPO at our reference hospital from July 2015 to June 2016. Clinical data was collected, and the response to αEPO treatment was evaluated using the erythropoietin resistance index (ERI). The ERI was defined as the weekly weight-adjusted αEPO dose (U/kg per week)/hemoglobin level (g/dL). A longitudinal linear regression model was fitted with random effects to verify ...
Source: Brazilian Journal of Medical and Biological Research - May 10, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

LncRNA HULC mediates radioresistance via autophagy in prostate cancer cells.
Authors: Chen C, Wang K, Wang Q, Wang X Abstract Prostate cancer (PCa) is the second leading cause of cancer death in men. Irradiation is one of the available options for treatment of PCa, however, approximately 10-45% of PCa are resistant to irradiation. We aimed to explore the role of long non-coding RNA highly upregulated in liver cancer (HULC) in the sensitivity of PCa cells to irradiation. Survival rate, cell apoptosis, cycle, expressions of related proteins, and caspase-3 activity were assessed to explore the effects of HULC on sensitivity of PCa cells to irradiation. Expression of HULC in DU-145, PC3, LNCaP,...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Neonatal tobacco smoke reduces thermogenesis capacity in brown adipose tissue in adult rats.
Authors: Peixoto TC, Moura EG, Oliveira E, Younes-Rapozo V, Soares PN, Rodrigues VST, Santos TR, Peixoto-Silva N, Carvalho JC, Calvino C, Conceição EPS, Guarda DS, Claudio-Neto S, Manhães AC, Lisboa PC Abstract Maternal smoking is a risk factor for progeny obesity. We have previously shown, in a rat model of neonatal tobacco smoke exposure, a mild increase in food intake and a considerable increase in visceral adiposity in the adult offspring. Males also had secondary hyperthyroidism, while females had only higher T4. Since brown adipose tissue (BAT) hypofunction is related to obesity, here we ...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Influence of expiratory positive airway pressure on cardiac autonomic modulation at rest and in submaximal exercise in COPD patients.
Authors: da L Goulart C, San Martin EA, Mansour KMK, Schneiders PB, da Silva ALG Abstract The aim of this study was to evaluate the effect of expiratory positive airway pressure (EPAP) on heart rate variability (HRV) indices at rest and during 6-min walk test (6MWT) in chronic obstructive pulmonary disease (COPD) patients. Fifteen moderate to severe COPD patients were randomized and evaluated with and without (Non-EPAP) a 5 cmH2O EPAP device. Respiratory rate (RR) was collected at rest (5 min), during the 6MWT (5 min), and at recovery (5 min). Indices of HRV were computed in the time domain, in the frequency domain...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

TNF- α inhibits SCF, ghrelin, and substance P expressions through the NF-κB pathway activation in interstitial cells of Cajal.
In conclusion, TNF-α down-regulated the expressions of SCF, ghrelin, and substance P via the activation of the NF-κB pathway in ICC. PMID: 29694505 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Dietary intake and eating behavior in depot medroxyprogesterone acetate users: a systematic review.
Authors: Silva P, Qadir S, Fernandes A, Bahamondes L, Peipert JF Abstract Because of weight gain, women often discontinue hormonal contraception, especially depot medroxyprogesterone acetate (DMPA). Our objective was to conduct a systematic review of studies describing dietary intake or eating behavior in DMPA users to understand whether the use of DMPA is associated with changes in dietary habits and behaviors leading to weight gain. We searched the PubMed, POPLINE, CENTRAL Cochrane, Web of Science, and EMBASE databases for reports published in English between 1980 and 2017 examining dietary intake or eating behav...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Protective effect of yerba mate intake on the cardiovascular system: a post hoc analysis study in postmenopausal women.
Authors: da Veiga DTA, Bringhenti R, Copes R, Tatsch E, Moresco RN, Comim FV, Premaor MO Abstract The prevalence of cardiovascular and metabolic diseases is increased in postmenopausal women, which contributes to the burden of illnesses in this period of life. Yerba mate (Ilex paraguariensis) is a native bush from Southern South America. Its leaves are rich in phenolic components, which may have antioxidant, vasodilating, hypocholesterolemic, and hypoglycemic proprieties. This post hoc analysis of the case-control study nested in the Obesity and Bone Fracture Cohort evaluated the consumption of yerba mate and the p...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Andrographolide protects mouse astrocytes against hypoxia injury by promoting autophagy and S100B expression.
Authors: Du J, Zhang C, Na X, Li A, Zhang Q, Li K, Ding Y Abstract Andrographolide (ANDRO) has been studied for its immunomodulation, anti-inflammatory, and neuroprotection effects. Because brain hypoxia is the most common factor of secondary brain injury after traumatic brain injury, we studied the role and possible mechanism of ANDRO in this process using hypoxia-injured astrocytes. Mouse cortical astrocytes C8-D1A (astrocyte type I clone from C57/BL6 strains) were subjected to 3 and 21% of O2 for various times (0-12 h) to establish an astrocyte hypoxia injury model in vitro. After hypoxia and ANDRO administratio...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPAR γ expression.
In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to eva...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Exploration of gene functions for esophageal squamous cell carcinoma using network-based guilt by association principle.
Authors: Wu W, Huang B, Yan Y, Zhong ZQ Abstract Gene networks have been broadly used to predict gene functions based on guilt by association (GBA) principle. Thus, in order to better understand the molecular mechanisms of esophageal squamous cell carcinoma (ESCC), our study was designed to use a network-based GBA method to identify the optimal gene functions for ESCC. To identify genomic bio-signatures for ESCC, microarray data of GSE20347 were first downloaded from a public functional genomics data repository of Gene Expression Omnibus database. Then, differentially expressed genes (DEGs) between ESCC patients an...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury.
In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. H9c2 cells and human cardiomyocytes (HCM) were subjected to either hypoxia/reoxygenation (H/R), I/R or normal conditions (normoxia). The expression levels of lncRNA-ROR were detected in serum of myocardial I/R injury patients, H9c2 cells, and HCM by qRT-PCR. Then, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were measured by kits. Cell viability, apoptosis, apoptosis-associated factors, and p38/MAPK pathway were examine...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Quality of life on hemodialysis and inflammation: a descriptive analysis.
Authors: Bacci MR, Adami F, Figueiredo FWS, Alves BCA, da Veiga GL, Fonseca FLA Abstract Chronic kidney disease (CKD) is highly prevalent worldwide. Patients with CKD on hemodialysis are more likely to present behavioral changes and worse quality of life as a result of their routine and complications. They also have higher levels of cytokines. The aim of this study is to assess the relationship between the inflammatory profile and quality of life measured by KDOQL-SF36 in hemodialysis outpatients. Patients older than 21 years of age and on routine hemodialysis for at least 6 months with treatment on a regular weekl...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

miR-27a protects human mitral valve interstitial cell from TNF- α-induced inflammatory injury via up-regulation of NELL-1.
This study aimed to investigate the functional impacts of miR-27a on TNF-α-induced inflammatory injury in human mitral valve interstitial cells (hMVICs). hMVICs were subjected to 40 ng/mL TNF-α for 48 h, before which the expressions of miR-27a and NELL-1 in hMVICs were altered by stable transfection. Trypan blue staining, BrdU incorporation assay, flow cytometry detection, ELISA, and western blot assay were performed to detect cell proliferation, apoptosis, and the release of proinflammatory cytokines. We found that miR-27a was lowly expressed in response to TNF-α exposure in hMVICs. Overexpression of miR...
Source: Brazilian Journal of Medical and Biological Research - April 26, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Advancement in liver laparoscopic resection - development of a new surgical device.
Authors: Vavra P, Karnik L, Skrobankova M, Jurcikova J, Ihnat P, Zonca P, Peteja M, El-Gendi A, Czudek S Abstract Liver resection is the standard treatment for any liver lesion. Laparoscopic liver resection is associated with lower intra-operative blood loss and fewer complications than open resection. Access to the posterior part of the right liver lobe is very uncomfortable and difficult for surgeons due the anatomic position, especially when employing laparoscopic surgery. Based on these experiences, a new laparoscopic device was developed that is capable of bending its long axis and allowing the application of ...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells.
Authors: Rolim PM, Fidelis GP, Padilha CEA, Santos ES, Rocha HAO, Macedo GR Abstract Melon (Cucumis melo L.) has high economic value and in recent years, its production has increased; however, part of the fruit is wasted. Usually, inedible parts such as peel and seeds are discarded during processing and consumption. Extracts of melon residues were prepared and their phenolic compounds, antioxidants and antiproliferative activities were evaluated. Total phenolic compounds were found in hydroethanolic, hydromethanolic, and aqueous extracts, especially for melon peel (1.016 mg gallic acid equivalent/100 g). Flavonoids...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Urinary semaphorin 3A as an early biomarker to predict contrast-induced acute kidney injury in patients undergoing percutaneous coronary intervention.
This study enrolled 168 patients with stable angina undergoing elective PCI. Serial urine samples, obtained at baseline and 2, 6, 12, 24, 36, and 48 h post-PCI were analyzed by semaphorin 3A and neutrophil gelatinase-associated lipocalin (NGAL) ELISA kit. AKI was defined as an increase in serum creatinine beyond 50% according to the RIFLE classification system. Receiver operator characteristic (ROC) curve analyses identified optimal semaphorin 3A and NGAL values for diagnosing CI-AKI. CI-AKI occurred in 20 of 168 patients. There were no significant differences in the baseline clinical characteristics and angiographic findi...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Inflammatory biomarkers responses after acute whole body vibration in fibromyalgia.
In conclusion, a single acute session of mild and short WBV can improve the inflammatory status in patients with FM, reaching values close to those of matched HW at their basal status. The neuroendocrine mechanism seems to be an exercise-induced modulation towards greater adaptation to stress response in these patients. PMID: 29513791 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Polydatin inhibits cell proliferation, invasion and migration, and induces cell apoptosis in hepatocellular carcinoma.
Authors: Jiao Y, Wu Y, Du D Abstract Polydatin, a small molecule from Polygonum cuspidatum, has many biological functions, particularly anti-cancer effects. However, the anti-cancer effects of polydatin in hepatocellular carcinoma (HCC) have not been examined yet. In the present study, MTT assay, BrdU assay, transwell invasion assay, and wound healing assay were performed to determine cell proliferation, invasion and migration. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Quantitative real-time PCR and western blotting assays were used to determine mRNA and protein expression levels. Xenograf...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Tea polyphenols induce S phase arrest and apoptosis in gallbladder cancer cells.
Authors: Wang J, Pan Y, Hu J, Ma Q, Xu Y, Zhang Y, Zhang F, Liu Y Abstract Gallbladder cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-cancer effect of TPs in human gallbladder cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

The hepatic ectonucleotide pyrophosphatase/phosphodiesterase 1 gene mRNA abundance is reduced by insulin and induced by dexamethasone.
In conclusion, we demonstrated for the first time that hepatic Enpp1 is regulated in the cycle of fasting and refeeding, a process that might be attributed to insulin-reduced Enpp1 expression. This insulin-reduced Enpp1 expression might play a role in the development of complications in diabetic patients. PMID: 29513794 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research

Structural heart disease as the cause of syncope.
We described the clinical evolution of patients with structural heart disease presenting at the emergency room with syncope. Patients were stratified according to their syncope etiology and available scores for syncope prognostication. Cox proportional hazard models were used to investigate the relationship between etiology of the syncope and event-free survival. Of the 82,678 emergency visits during the study period, 160 (0.16%) patients were there due to syncope, having a previous diagnosis of structural heart disease. During the median follow-up of 33.8±13.8 months, mean age at the qualifying syncope event was 68...
Source: Brazilian Journal of Medical and Biological Research - March 9, 2018 Category: Research Tags: Braz J Med Biol Res Source Type: research