Treatment with zolpidem after ethanol administration potentiates the expression of ethanol-induced behavioral sensitization in mice.
Authors: Brandão NRN, Libarino-Santos M, Marinho EAV, Oliveira TS, Borges ALN, Oliveira AP, Oliveira-Campos D, Azevedo-Souza N, Santos VFL, Berro LF, Oliveira-Lima AJ Abstract Contradictory findings suggest that the behavioral and abuse-related effects of ethanol are mediated by its action at α1 subunit-containing GABAA (α1GABAA) receptors. In the present study, we investigated the effects of a sub-chronic post-ethanol administration treatment with zolpidem, an α1-preferring positive allosteric modulator at GABAA receptors, on the subsequent expression of ethanol-induced behavioral sensitiz...
Source: Brazilian Journal of Medical and Biological Research - July 3, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Long non-coding RNA LINC01268 promotes cell growth and inhibits cell apoptosis by modulating miR-217/SOS1 axis in acute myeloid leukemia.
This study offers a novel molecular mechanism for a better understanding of the pathology of AML. LINC01268 could be considered as a potential biomarker for the therapy and diagnosis of AML. PMID: 32609259 [PubMed - as supplied by publisher] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - July 3, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Predictors of chest drainage of pneumothorax in neonates.
Authors: Tan YL, Zhan Y, Geng J, Chen W, Guo WL Abstract This is a retrospective, single-center observational study to explore the predictors of chest drainage for neonatal pneumothorax. A total of 183 neonates (age ≤28 days) who presented to the Children's Hospital of Soochow University between January 1, 2015 and December 31, 2018 for pneumothorax or developed pneumothorax during a hospital stay were included. Demographic data, clinical presentation, and imaging characteristics of neonatal pneumothorax were collected and analyzed. We used univariate and multivariate logistic regression analyses to determine si...
Source: Brazilian Journal of Medical and Biological Research - July 3, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

A method to assess heart rate variability in neonate rats: validation in normotensive and hypertensive animals.
In conclusion, the ECG setup here described may be used to record RR intervals to assess HRV in neonate rats, thus detecting early impairment of HRV in hypertensive newborns. PMID: 32609261 [PubMed - as supplied by publisher] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - July 3, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Hyaluronic acid versus dexamethasone for the treatment of recurrent aphthous stomatitis in children: efficacy and safety analysis.
The objective of this study was to compare the safety and efficacy of 0.2% hyaluronic acid (HA) topical gel and dexamethasone topical ointment in the treatment of recurrent aphthous ulcers (RAU) in children. This retrospective observational study included 104 patients who had more than two episodes of oral aphthous ulcers per year and were treated with HA (n=52) or dexamethasone (n=52) from August 15, 2014 to September 3, 2018. Therapy efficacy was evaluated based on the ulcer size and pain score before versus 7 days after either therapy. The paired t-test, chi-squared test, and independent t-test were utilized for statist...
Source: Brazilian Journal of Medical and Biological Research - July 3, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Biomechanical and biochemical investigation of erythrocytes in late stage human leptospirosis.
Authors: Silva JAX, Albertini AVP, Fonseca CSM, Silva DCN, Carvalho VCO, Lima VLM, Fontes A, Costa EVL, Nogueira RA Abstract Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira, which can cause lipid changes in the erythrocyte membrane. Optical tweezers were used to characterize rheological changes in erythrocytes from patients with leptospirosis in the late stage. Biochemical methods were also used for quantification of plasma lipid, erythrocyte membrane lipid, and evaluation of liver function. Our data showed that the mean elastic constant of erythrocytes from patients with leptospirosi...
Source: Brazilian Journal of Medical and Biological Research - June 25, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Subjective time under altered states of consciousness in ayahuasca users in shamanistic rituals involving music.
Authors: Campagnoli APS, Pereira LAS, Bueno JLO Abstract Ayahuasca is described as a hallucinogenic substance whose property is to alter the subjective experience of time and impair the perception of the passage of time during stimuli of more than two to three seconds. The dose-dependent effects of two concentrations of ayahuasca in the ritualistic context were investigated employing temporal reproduction tasks in participants experienced in shamanistic ayahuasca rituals. The study was conducted on nine healthy volunteers who ingested two doses of ayahuasca at two times during a ritual session. The doses of each se...
Source: Brazilian Journal of Medical and Biological Research - June 25, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Modulation of Wnt/ β-catenin signaling in IL-17A-mediated macrophage polarization of RAW264.7 cells.
This study thus revealed a novel function of Wnt/β-catenin signaling in regulating IL-17A-altered macrophage polarization. PMID: 32578719 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - June 25, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Low expression of microRNA-328 can predict sepsis and alleviate sepsis-induced cardiac dysfunction and inflammatory response.
This study investigated the clinical value of microRNA-328 (miR-328) in sepsis and its role in cardiac dysfunction and inflammation caused by sepsis. The expression level of miR-328 in the serum of the subjects was detected by qRT-PCR. Receiver operating characteristic (ROC) curve measured the diagnostic value of miR-328 in sepsis. Rat sepsis model was established to detect left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and maximal rate of increase/decrease of left ventricular pressure (±dp/dtmax). Myocardial injury markers serum cardiac troponin I (cTnI), myocardial kina...
Source: Brazilian Journal of Medical and Biological Research - June 25, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Temporomandibular inflammation mobilizes parvalbumin and FosB/deltaFosB neurons of amygdala and dorsal raphe.
In conclusion, altered activity of the amygdala and DR was detected during persistent inflammatory nociception in the temporomandibular joint. These regions may be essential for both sensory and affective dimensions of orofacial pain. PMID: 32578721 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - June 25, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

lncRNA PCGEM1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma.
This study demonstrated the anti-inflammatory and lung-protective effects of montelukast sodium on CVA, which was strengthened by overexpression of PCGEM1. Findings in this study highlighted a potential anti-asthmatic target of montelukast sodium. PMID: 32520202 [PubMed - as supplied by publisher] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Detoxification effects of long-chain versus a mixture of medium- and long-chain triglyceride-based fat emulsion on propafenone poisoning.
Authors: An X, Mei Y, Sun H, Zhang J Abstract In the present study, we aimed to compare the detoxifying effects of two fat emulsions containing either long-chain triglyceride or a mixture of medium-chain and long-chain triglycerides in the propafenone-poisoned rat model. Rats were randomly divided into 3 groups according to the fat emulsions used: long-chain triglyceride-based fat emulsion (LL) group; medium-chain and long-chain triglyceride-based fat emulsion (ML) group; normal saline (NS) group. Propafenone was continuously pumped (velocity=70 mg/kg per h) until the mean blood pressure dropped to 50% of basal lev...
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Heart failure with preserved ejection fraction: an update on pathophysiology, diagnosis, treatment, and prognosis.
Authors: Ma C, Luo H, Fan L, Liu X, Gao C Abstract Heart failure (HF) with preserved ejection fraction (HFpEF) is a clinical syndrome in which patients have symptoms and signs of HF with normal or near-normal left ventricular ejection fraction (LVEF ≥50%). Roughly half of all patients with HF worldwide have an LVEF ≥50% and nearly half have an LVEF
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Reaching task performance is associated to neuromuscular junction adaptations in rats with induced diabetes mellitus.
In conclusion, induced DM caused NMJ retraction and muscular atrophy in muscles involved in reaching task performance. Induced DM caused significantly lower motor performance, especially in the final moments of evaluation, when DM compromised the tropism of the muscular tissue. PMID: 32520205 [PubMed - as supplied by publisher] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

MicroRNA-424-5p inhibits the proliferation, migration, and invasion of nasopharyngeal carcinoma cells by decreasing AKT3 expression.
This study examined the expression and potential mechanism of microRNA (miRNA)-424-5p in nasopharyngeal carcinoma (NPC). NPC tissues were collected from 40 patients who were enrolled in the study, and skin samples were collected from 26 healthy subjects during plastic surgery as controls. We performed various in vitro assays using miR-424-5p to examine its function in primary NPC-1 cells. Bioinformatics was employed to analyze potential target genes and signaling pathways of miR-424-5p. We found that miR-424-5p expression in NPC tissues is downregulated and negatively correlated with lymph node metastasis and clinical stag...
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Relationship between PI3K/mTOR/RhoA pathway-regulated cytoskeletal rearrangements and phagocytic capacity of macrophages.
The objective of this study was to investigate the relationship between PI3K/mTOR/RhoA signaling regulated cytoskeletal rearrangements and phagocytic capacity of macrophages. RAW264.7 macrophages were divided into four groups; blank control, negative control, PI3K-RNAi, and mTOR-RNAi. The cytoskeletal changes in the macrophages were observed. Furthermore, the phagocytic capacity of macrophages against Escherichia coli is reported as mean fluorescence intensity (MFI) and percent phagocytosis. Transfection yielded 82.1 and 81.5% gene-silencing efficiencies against PI3K and mTOR, respectively. The PI3K-RNAi group had lower mR...
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Knocking down LSD1 inhibits the stemness features of colorectal cancer stem cells.
In this study, our objectives included: 1) to isolate pure CD133+ and CD133- cells from SW620 cell line; 2) to investigate the effect of LSD1 on the characteristics of CD133+ stem cancer cells by knocking down the target gene. Results suggested that the SW620 cell line had both CD133+ and CD133- subsets. The CD133+ subset exhibited more CSC-like characteristics compared with the CD133- subset with higher viability, colony formation rate, migration and invasion rate, resistance to anti-cancer drugs, and apoptosis in vitro. The CD133+ also induced faster tumor formation and larger tumors in vivo. In the LSD1-knockdown CD133+...
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Ophiopogonin D of Ophiopogon japonicus ameliorates renal function by suppressing oxidative stress and inflammatory response in streptozotocin-induced diabetic nephropathy rats.
Authors: Qiao Y, Jiao H, Wang F, Niu H Abstract Ophiopogonin D (OP-D) is the principal pharmacologically active ingredient from Ophiopogon japonicas, which has been demonstrated to have numerous pharmacological activities. However, its protective effect against renal damage in streptozotocin (STZ)-induced diabetic nephropathy (DN) rats remains unclear. The present study was performed to investigate the protective effect of OP-D in the STZ-induced DN rat model. DN rats showed renal dysfunction, as evidenced by decreased serum albumin and creatinine clearance, along with increases in serum creatinine, blood urea nitr...
Source: Brazilian Journal of Medical and Biological Research - June 11, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Early and late neuropathological features of meningoencephalitis associated with Maraba virus infection.
Authors: Maia-Farias A, Lima CM, Freitas PSL, Diniz DG, Rodrigues APD, Quaresma JAS, Diniz CWP, Diniz JA Abstract Maraba virus is a member of the genus Vesiculovirus of the Rhabdoviridae family that was isolated in 1983 from sandflies captured in the municipality of Maraba, state of Pará, Amazônia, Brazil. Despite 30 years having passed since its isolation, little is known about the neuropathology induced by the Maraba virus. Accordingly, in this study the histopathological features, inflammatory glial changes, cytokine concentrations, and nitric oxide activity in the encephalon of adult mice subjected...
Source: Brazilian Journal of Medical and Biological Research - April 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Acute effect of passive cycle-ergometry and functional electrical stimulation on nitrosative stress and inflammatory cytokines in mechanically ventilated critically ill patients: a randomized controlled trial.
The objective of this study was to assess the effect of functional electrical stimulation (FES) and passive cycle ergometry (PCE) on the nitrous stress and inflammatory cytometry in critically ill patients. This was a controlled, randomized, open clinical trial carried out in a 16-bed intensive care unit. The patients were randomized into four groups: Control group (n=10), did not undergo any therapeutic intervention during the study; PCE group (n=9), lower-limb PCE for 30 cycles/min for 20 min; FES group (n=9), electrical stimulation of quadriceps muscle for 20 min; and FES with PCE group (n=7), patients underwent PCE and...
Source: Brazilian Journal of Medical and Biological Research - April 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition.
In this study, CCK-8, EdU immunofluorescence, colony formation, wound healing, and migration assays were used to detect the effects of puerarin on HCC cells. We further analyzed the relationship between puerarin and miR-21/PTEN/EMT markers in HCC cell lines. Our results showed that HCC cell proliferation, migration, invasion, tumor formation, and metastasis were reduced by puerarin in vitro and in vivo. Additionally, puerarin inhibited the EMT process of HCC by affecting the expression of Slug and Snail. Moreover, oncogenic miR-21 was inhibited by puerarin, coupled with an increase in the tumor suppressor gene PTEN. Increa...
Source: Brazilian Journal of Medical and Biological Research - April 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

VPA/PLGA microfibers produced by coaxial electrospinning for the treatment of central nervous system injury.
In this study, VPA was encapsulated in poly(lactic-co-glycolic acid) (PLGA) microfibers by the coaxial electrospinning technique. Fibers showed continuous and cylindrical morphology, randomly oriented fibers, and compatible morphological and mechanical characteristics for application in SCI. Drug-release analysis indicated a rapid release of VPA during the first day of the in vitro test. The coaxial fibers containing VPA supported adhesion, viability, and proliferation of PC12 cells. In addition, the VPA/PLGA microfibers induced the reduction of PC12 cell viability, as has already been described in the literature. The biom...
Source: Brazilian Journal of Medical and Biological Research - April 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

A poor prognosis in human hepatocellular carcinoma is associated with low expression of DPP4.
This study aimed to explore the prognostic role of dipeptidyl peptidase 4 (DPP4) expression in hepatocellular carcinoma (HCC). DPP4 expression was measured in formalin-fixed paraffin-embedded specimens that were gathered from 327 HCC patients. Immunohistochemistry analyses were utilized to examine DPP4 expression characteristics and prognostic values (overall survival (OS) and time to recurrence) of DDP4 in HCC tissues. In addition, a patient-derived xenograft (PDX) model was used to assess the correlation between DPP4 expression and tumor growth in vivo. DPP4 was expressed in low levels in HCC tissues in contrast to paire...
Source: Brazilian Journal of Medical and Biological Research - April 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

lncRNA ZEB1-AS1 inhibits high glucose-induced EMT and fibrogenesis by regulating the miR-216a-5p/BMP7 axis in diabetic nephropathy.
Authors: Meng Q, Zhai X, Yuan Y, Ji Q, Zhang P Abstract Diabetic nephropathy (DN) is one of the leading causes of mortality in diabetic patients. Long non-coding RNA zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) plays a crucial role in the development of various diseases, including DN. However, the molecular mechanism of ZEB1-AS1 in DN pathogenesis remains elusive. An in vitro DN model was established by treating HK-2 cells with high glucose (HG). Quantitative polymerase chain reaction (qRT-PCR) was utilized to detect the expression levels of ZEB1-AS1, microRNA-216a-5p (miR-216a-5p), and bone morphoge...
Source: Brazilian Journal of Medical and Biological Research - April 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Prognostic value of increased expression of RBM8A in gastric cancer.
This study was designed to investigate the expression of RBM8A protein in patients with gastric cancer (GC) and to explore its correlation with clinical pathological features as well as prognosis. One hundred pairs of gastric carcinoma tissues and adjacent tissues from patients undergoing gastrectomy for GC were included in this study. The protein expression level of RBM8A was determined by immunohistochemistry using tissue microarrays. We also detected the mRNA expression level of RBM8A in 16 pairs of gastric carcinoma tissues and adjacent tissues. Meanwhile, we predicted the potential correlation between RBM8A and tumor ...
Source: Brazilian Journal of Medical and Biological Research - April 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

SAD-B modulates epileptic seizure by regulating AMPA receptors in patients with temporal lobe epilepsy and in the PTZ-induced epileptic model.
Authors: Li R, He M, Wu B, Zhang P, Zhang Q, Chen Y Abstract α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors are the predominant mediators of glutamate-induced excitatory neurotransmission. It is widely accepted that AMPA receptors are critical for the generation and spread of epileptic seizure activity. Dysfunction of AMPA receptors as a causal factor in patients with intractable epilepsy results in neurotransmission failure. Brain-specific serine/threonine-protein kinase 1 (SAD-B), a serine-threonine kinase specifically expressed in the brain, has been shown to regulate AMPA receptor-m...
Source: Brazilian Journal of Medical and Biological Research - April 9, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Ingestion of a moderate dose of alcohol enhances physical exercise-induced changes in blood lactate concentration.
Authors: Teixeira-Coelho F, Santos DFC, Santos GA, Sousa TF, Moreira SR, Souza MVC, Wanner SP Abstract The consumption of alcoholic beverages influences carbohydrate and lipid metabolism, although it is not yet clear whether metabolism during physical exercise at different intensities is also affected. This was the objective of the present study. Eight young and healthy volunteers performed a treadmill test to identify the running speed corresponding to a lactate concentration of 4 mM (S4mM). At least 48 h later, they were subjected to two experimental trials (non-alcohol or alcohol) in which they performed two 1-k...
Source: Brazilian Journal of Medical and Biological Research - April 9, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effects of Rab7 gene up-regulation on renal fibrosis induced by unilateral ureteral obstruction.
Authors: Xu Q, Liu L, Yang Y, Wang Z, Cai Y, Hong T, Chen P Abstract Rab7, an important member of the Rab family, is closely related to autophagy, endocytosis, apoptosis, and tumor suppression but few studies have described its association with renal fibrosis. In the early stage, our group studied the effects of Rab7 on production and degradation of extracellular matrix in hypoxic renal tubular epithelial cells. Because cell culture in vitro is different from the environment in vivo, it is urgent to understand the effects in vivo. In our current study, we established a renal fibrosis model in Rab7-knock-in mice (pr...
Source: Brazilian Journal of Medical and Biological Research - April 9, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Critical role of metabotropic glutamate receptor 4 in bone marrow-derived dendritic cells in the Th17 cell differentiation and the melanogenesis of B16 cells.
Authors: Zhao G, Zhou W, Liu Y, Wang Y, Li Z, Song Z Abstract Vitiligo is an acquired pigmentary disorder resulting from selective destruction of melanocytes. Emerging studies have suggested that T helper cell 17 (Th17) is potentially implicated in vitiligo development and progression. It was recently discovered that metabotropic glutamate receptor 4 (mGluR4) can modulate Th17-mediated adaptive immunity. However, the influence of mGluR4 on melanogenesis of melanocytes has yet to be elucidated. In the present study, we primarily cultured mouse bone marrow-derived dendritic cells (BMDC) and then knocked down and over...
Source: Brazilian Journal of Medical and Biological Research - April 9, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Manual and semiautomatic segmentation of bone sarcomas on MRI have high similarity.
Authors: Dionísio FCF, Oliveira LS, Hernandes MA, Engel EE, Rangayyan RM, Azevedo-Marques PM, Nogueira-Barbosa MH Abstract The aims of this study were to evaluate the intra- and interobserver reproducibility of manual segmentation of bone sarcomas in magnetic resonance imaging (MRI) studies and to compare manual and semiautomatic segmentation methods. This retrospective study included twelve osteosarcoma and eight Ewing sarcoma MRI studies performed prior to any therapeutic intervention. All cases were histopathologically confirmed. Three radiologists used 3D-Slicer software to perform manual segmentation of...
Source: Brazilian Journal of Medical and Biological Research - February 6, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

The contribution of Latin American research to HPV epidemiology and natural history knowledge.
Authors: Sichero L, Picconi MA, Villa LL Abstract Our aim was to review the major contributions of studies conducted in different Latin American (LA) countries to the field of human papillomavirus (HPV) epidemiology, natural history, risk of disease, and prevention strategies, mainly in the uterine cervix. Although cytological screening is established in several countries in LA, incidence and mortality rates from cervical cancer (CC) are still extremely high. Finally, data from large cohort studies conducted in LA countries provided seminal data to propose primary and secondary prevention modalities: the HPV vaccin...
Source: Brazilian Journal of Medical and Biological Research - February 6, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Inhibition of miR-383 suppresses oxidative stress and improves endothelial function by increasing sirtuin 1.
Authors: Hu B, Gong Z, Bi Z Abstract Previous research has shown that suppression of miR-383 can prevent inflammation of the endothelium, as well as postpone the development of atherosclerosis. However, the role of miR-383 in endothelial cell apoptosis in diabetes remains unclear. The aim of this study was to investigate the function of miR-383 in high glucose-induced apoptosis and oxidative stress in endothelial cells. A series of experiments involving qualitative polymerase chain reaction, cell transfection, luciferase assay, assessment of cell death, detection of catalase and superoxide dismutase concentrations,...
Source: Brazilian Journal of Medical and Biological Research - January 30, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

A review on edible vaccines and their prospects.
This article focused on the conception of edible vaccines highlighting the various ways by which vaccines can be delivered. PMID: 31994600 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - January 30, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Aliskiren attenuates cardiac dysfunction by modulation of the mTOR and apoptosis pathways.
Authors: Zhao Z, Liu H, Guo D Abstract Aliskiren (ALS) is well known for its antihypertensive properties. However, the potential underlying the molecular mechanism and the anti-hypertrophic effect of ALS have not yet been fully elucidated. The aim of the present study was to investigate the role of ALS in mammalian target of rapamycin (mTOR) and apoptosis signaling using in vivo and in vitro models of cardiac hypertrophy. A rat model of cardiac hypertrophy was induced by isoproterenol treatment (5 mg·kg-1·day-1) for 4 weeks, with or without ALS treatment at 20 mg·kg-1·day-1. The expressi...
Source: Brazilian Journal of Medical and Biological Research - January 30, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Anti-placental growth factor antibody ameliorates hyperoxia-mediated impairment of lung development in neonatal rats.
In conclusion, PGF overexpression impairs lung development in newborn rats while its inhibition using an anti-PGF antibody ameliorates the same. These results provided new insights for the clinical management of bronchopulmonary dysplasia in premature infants. PMID: 31994602 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - January 30, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

miR-26a-5p protects against myocardial ischemia/reperfusion injury by regulating the PTEN/PI3K/AKT signaling pathway.
Authors: Xing X, Guo S, Zhang G, Liu Y, Bi S, Wang X, Lu Q Abstract Reperfusion strategies in acute myocardial infarction (AMI) can cause a series of additional clinical damage, defined as myocardial ischemia/reperfusion (I/R) injury, and thus there is a need for effective therapeutic methods to attenuate I/R injury. miR-26a-5p has been proven to be an essential regulator for biological processes in different cell types. Nevertheless, the role of miR-26a-5p in myocardial I/R injury has not yet been reported. We established an I/R injury model in vitro and in vivo. In vitro, we used cardiomyocytes to simulate I/R in...
Source: Brazilian Journal of Medical and Biological Research - January 30, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Retraction notice for: "Risk factors for early-onset ventilator-associated pneumonia in aneurysmal subarachnoid hemorrhage patients" [Braz J Med Biol Res (2018) 51(7): e6830].
Retraction notice for: "Risk factors for early-onset ventilator-associated pneumonia in aneurysmal subarachnoid hemorrhage patients" [Braz J Med Biol Res (2018) 51(7): e6830]. Braz J Med Biol Res. 2020 Jan 13;53(1):e6830retraction Authors: Abstract [This retracts the article doi: 10.1590/1414-431X20176830]. PMID: 31939596 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - January 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer.
Authors: Song L, Wang L, Pan X, Yang C Abstract Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarifi...
Source: Brazilian Journal of Medical and Biological Research - January 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents.
The objective was the extension of the evaluation of protein fractions in the field up to 230 kDa to permit possible identification of some fractions that are insufficiently studied. The gel capillary electrophoresis method on the chip was performed using an Agilent 2100 bioassay with the 80 and 230-LabChip Protein kits. Interpretation of electrophoresis was performed using the Protein 2100 expert (Agilent) test software as follows: a) Protein 80 (peak size scale): 1.60, 3.5, 6.50, 15.00, 28.00, 46.00, 63.00, 95.00 kDa; b) Protein 230 (peak size scale): 4.50, 7.00, 15.00, 28.00, 46.00, 63.00, 95.00, 150.00, 240.00 kDa. The...
Source: Brazilian Journal of Medical and Biological Research - January 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Enhanced external counterpulsation improves cardiac function in Beagles after cardiopulmonary resuscitation.
Authors: Xiong J, Zhang W, Wei H, Li X, Dai G, Hu C Abstract The aim of this study was to investigate the influence of enhanced external counterpulsation (EECP) on the cardiac function of beagle dogs after prolonged ventricular fibrillation. Twenty-four adult male beagles were randomly divided into control and EECP groups. Ventricular fibrillation was induced in the animals for 12 min, followed by 2 min of cardiopulmonary resuscitation. They then received EECP therapy for 4 h (EECP group) or not (control group). The hemodynamics was monitored using the PiCCO2 system. Blood gas and hemorheology were assessed at base...
Source: Brazilian Journal of Medical and Biological Research - January 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Co-transfection of hepatocyte growth factor and truncated TGF- β type II receptor inhibit scar formation.
Co-transfection of hepatocyte growth factor and truncated TGF-β type II receptor inhibit scar formation. Braz J Med Biol Res. 2020;53(1):e9144 Authors: Xu JH, Zhao WY, Fang QQ, Wang XF, Zhang DD, Hu YY, Zheng B, Tan WQ Abstract Wound scarring remains a major challenge for plastic surgeons. Transforming growth factor (TGF)-β plays a key role in the process of scar formation. Previous studies have demonstrated that truncated TGF-β type II receptor (t-TGF-βRII) is unable to continue signal transduction but is still capable of binding to TGF-β, thereby blocking the TGF-β signa...
Source: Brazilian Journal of Medical and Biological Research - January 16, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Chlorophyll treatment combined with photostimulation increases glycolysis and decreases oxidative stress in the liver of type 1 diabetic rats.
Authors: Wunderlich ALM, Azevedo SCSF, Yamada LA, Bataglini C, Previate C, Campanholi KSS, Pereira PCS, Caetano W, Kaplum V, Nakamura CV, Nakanishi ABS, Comar JF, Pedrosa MMD, Godoi VAF Abstract Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dy...
Source: Brazilian Journal of Medical and Biological Research - December 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes.
In this study, the inflammatory phase of cutaneous lesions of hyperglycemic mice was modulated by the use of CAM, mostly regarding the cytokines IL-1α, IL-1β, TNF-α, G-CSF, and IL-10, resulting in better collagen III deposition. However, further studies are needed to better understand the healing stages associated with CAM use. PMID: 31859909 [PubMed - in process] (Source: Brazilian Journal of Medical and Biological Research)
Source: Brazilian Journal of Medical and Biological Research - December 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Feasibility of non-intubated anesthesia and regional block for thoracoscopic surgery under spontaneous respiration: a prospective cohort study.
Authors: Li H, Huang D, Qiao K, Wang Z, Xu S Abstract Data about the feasibility and safety of thoracoscopic surgery under non-intubated anesthesia and regional block are limited. In this prospective study, 57 consecutive patients scheduled for thoracoscopic surgery were enrolled. Patients were sedated with dexmedetomidine and anesthetized with propofol and remifentanil. Ropivacaine was used for intercostal nerve and paravertebral block. Lidocaine was used for vagal block. The primary outcomes were mean arterial pressure (MAP), heart rate (HR), oxygen saturation, and end-tidal carbon dioxide partial pressure (ETCO2...
Source: Brazilian Journal of Medical and Biological Research - December 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Use of flash glucose-sensing technology in patients with type 2 diabetes treated with liraglutide combined with CSII: a pilot study.
This study randomly assigned poorly controlled type 2 diabetes mellitus patients treated with metformin and multiple daily injections of insulin (n=60) to either continuous subcutaneous insulin infusion (CSII) treatment or CSII in combination with liraglutide (CSII+Lira) treatment for 14 days during hospitalization. GV was assessed using a FGM system; weight and cardiometabolic biomarkers were also evaluated. The coefficient of variation was significantly reduced in the CSII+Lira group (P10 mmol/L, P=0.028). The changes in mean of daily differences, interquartile range, and percentage of time in hypoglycemia (13.9 mmol/L) ...
Source: Brazilian Journal of Medical and Biological Research - December 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice.
Authors: Araújo JMD, Silva LAS, Felix FB, Camargo EA, Grespan R Abstract Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 recep...
Source: Brazilian Journal of Medical and Biological Research - December 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effect of fasudil on experimental autoimmune neuritis and its mechanisms of action.
This study aimed to investigate the therapeutic effect of fasudil on treating experimental autoimmune neuritis (EAN). Twenty-four EAN mice were randomly assigned to fasudil treatment (Fasudil group) or saline treatment (EAN model group) for 28 days. Clinical symptom score was evaluated every other day; inflammatory cell infiltration, demyelination, anti-myelin basic protein (MBP), inflammatory cytokines, inducible nitric oxide synthase (iNOS), and arginase-1 were detected in sciatic nerves at day 28. Th1, Th2, Th17, and Tregs proportions in splenocytes were detected at day 28. Clinical symptom score was found to be attenua...
Source: Brazilian Journal of Medical and Biological Research - December 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways.
Authors: Yang Z, Zhang H, An M, Bian M, Song M, Guo X, Liu Q, Qiu M Abstract Total Panax notoginseng saponin (TPNS) is the main bioactivity compound derived from the roots and rhizomes of Panax notoginseng (Burk.) F.H. Chen. The aim of this study was to investigate the effectiveness of TPNS in treating vascular neointimal hyperplasia in rats and its mechanisms. Male Sprague-Dawley rats were randomly divided into five groups, sham (control), injury, and low, medium, and high dose TPNS (5, 10, and 20 mg/kg). An in vivo 2F Fogarty balloon-induced carotid artery injury model was established in rats. TPNS significantly ...
Source: Brazilian Journal of Medical and Biological Research - December 21, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Retrospective analysis of efficacy, safety, and prognostic factors in a cohort of Chinese hepatocellular carcinoma patients treated with drug-eluting bead transarterial chemoembolization.
Authors: Chen S, Ji R, Shi X, Wang Z, Zhu D Abstract The aim of our study was to assess the efficacy, safety, and prognostic factors of drug-eluting bead transarterial chemoembolization (DEB-TACE) in Chinese hepatocellular carcinoma (HCC) patients. Patients (n=102) diagnosed as primary HCC were consecutively enrolled in this retrospective cohort study. Treatment responses were assessed following the modified Response Evaluation Criteria in Solid Tumors. Progression-free survival (PFS) and overall survival (OS) were evaluated, and adverse events (AEs) as well as liver function-related laboratory indexes of all DEB-T...
Source: Brazilian Journal of Medical and Biological Research - December 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Effects of physical exercise on skeletal muscles of rats with cerebral ischemia.
Authors: Melo RTR, Damázio LCM, Lima MC, Pereira VG, Okano BS, Monteiro BS, Natali AJ, Carlo RJD, Maldonado IRSC Abstract Physical exercise is a known preventive and therapeutic alternative for several cerebrovascular diseases. Therefore, the objective of the present study was to evaluate the motor performance and histomorphometry of the biceps brachii, soleus, and tibialis anterior muscles of rats submitted to a treadmill training program prior to the induction of cerebral ischemia via occlusion of the middle cerebral artery (OMCA). A total of 24 Wistar rats were distributed into four groups: Sham-Sed: sede...
Source: Brazilian Journal of Medical and Biological Research - December 6, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research