3-morpholinosydnonimine (SIN-1)-induced oxidative stress leads to necrosis in hypertrophic chondrocytes in vitro
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Ying He, Ying Zhang, Dan Zhang, Meng Zhang, Mengying Wang, Zhuocheng Jiang, Miguel Otero, Jinghong ChenAbstractChondrocyte is targeted for disruption in Osteoarthritis (OA) and Kashin-Beck Disease (KBD), and chondrocyte death in cartilage may contribute to the progression of OA and KBD. Oxidative stress leads to increased risk for OA. Previous work in our laboratory implicates oxidative stress as a potential mediator in children with KBD. While these studies suggest a role for oxidative stress in the modulation of OA and KBD, the ...
Source: Biomedicine and Pharmacotherapy - July 30, 2018 Category: Drugs & Pharmacology Source Type: research

Long noncoding RNA VPS9D1-AS1 overexpression predicts a poor prognosis in non-small cell lung cancer
In this study, we aimed to investigate its expression pattern and clinical significance and further evaluate its prognostic value for NSCLC. VPS9D1-AS1 expression was examined in 184 NSCLC patients using a highly sensitive in situ hybridization protocol (RNAscope), and the expression values were correlated with the clinicopathological features. Another cohort including 12 NSCLC patients was used to validate the differential expression of VPS9D1-AS1 by qRT-PCR. TCGA datasets were further used to validate the main findings. We found that the levels of VPS9D1-AS1 were significantly higher in cancer tissues than in paired norm...
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

Overexpression of MEG3 sensitizes colorectal cancer cells to oxaliplatin through regulation of miR-141/PDCD4 axis
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Hongjian Wang, Hui Li, Lei Zhang, Desheng YangAbstractThe existence of drug resistance is the main reason for chemotherapeutic failure in malignancies. Long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) is implicated with tumorigenesis and chemoresistance. In the present study, we aimed to identify the role of MEG3 in oxaliplatin-resistant colorectal cancer (CRC) and its potential mechanisms. MEG3 was down-regulated in oxaliplatin-resistant CRC tissues and cell lines. Low MEG3 expression was correlated with poor progn...
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

Modulatory effect of eugenol on arginase, nucleotidase, and adenosine deaminase activities of platelets in a carrageenan-induced arthritis rat model: A possible anti-arthritic mechanism of eugenol
This study investigated the effect of eugenol on arginase, nucleotidase and adenosine deaminase activities in platelets of carrageenan-induced arthritic rat model to explain a possible anti-arthritic mechanism of eugenol. Fifty adult female rats (140–250 g) were divided into ten (10) groups (n = 5). Group I received oral administration of corn oil, group II received 2.50 mg/kg of eugenol, group III and IV rats received oral administration of 5.0 and 10.0 mg/kg of eugenol respectively, group V received 0.20 mg/kg of dexamethasone orally, group VI rats was injected with 1% carr...
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

Insights of Leishmania (Viannia) braziliensis infection in golden hamster (Mesocricetus auratus) intestine
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Amanda Gubert Alves dos Santos, Lainy Leiny de Lima, Camila Alves Mota, Marcelo Biondaro Gois, Andrea Claudia Bekner Silva Fernandes, Thaís Gomes Verzignassi Silveira, Debora de Mello Gonçales Sant’Ana, Gessilda de Alcântara Nogueira de MeloAbstractAimThe present study compared and evaluated morphological and quantitative alterations in the ileum of hamsters infected by two L. (V.) braziliensis strains isolated from patients with different lesion aspects and treatment responses.Main methodsHamsters were i...
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

miR-516b functions as a tumor suppressor by directly modulating CCNG1 expression in esophageal squamous cell carcinoma
ConclusionThe results demonstrated miR-516b functions as a tumor suppressor by directly modulating CCNG1 expression in ESCC cells, and may be a novel therapeutic and prognostic biomarker for ESCC.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

LncRNA SNHG16 reverses the effects of miR-15a/16 on LPS-induced inflammatory pathway
This study aims to explore a lncRNA can bind with miR-15a/16 in neonatal sepsis. Microarray analysis helped us found top ten lncRNAs which were downregulated in neonatal sepsis serum. Among these ten lncRNAs, SNHG16 was uncovered to significantly downregulated both miR-15a and miR-16. According to the result of subcellular fractionation assay, SNHG16 was mainly located in the cytoplasm of RAW264.7 cell, indicating the potential ceRNA role of SNHG16. Mechanism investigations revealed that SNHG16 could act as a ceRNA to upregulate TLR4 which is the target mRNA of miR-15a/16 cluster. At last, rescue assays demonstrated that S...
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

Crocin improves endometriosis by inhibiting cell proliferation and the release of inflammatory factors
This study was designed to assess the potential effects of crocin on endometriosis. We established the mice model of endometriosis and administrated crocin to the mice. We monitored the endometriotic lesion growth, PCNA and VEGF expression in the lesion. We tested the serum levels of inflammatory cytokines in crocin-treated endometriosis mice. Finally we tested the effect of crocin on endothelial cell apoptosis and proliferation, and cytokine production in LPS-stimulated human monocyte. Crocin inhibited lesion growth in endometriosis mice and prevented PCNA and VEGF expression in the lesions. Crocin decreased the levels of...
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

Anticonvulsant effect of anacardic acid in murine models: Putative role of GABAergic and antioxidant mechanisms
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Antonio Luiz Gomes, Jana Dimitrova Tchekalarova, Milena Atanasova, Keylla da Conceição Machado, Maria Alexsandra de Sousa Rios, Márcia Fernanda Paz Jardim, Mihnea-Alexandru Găman, Amelia Maria Găman, Santosh Yele, Manik Chandra Shill, Ishaq N. Khan, Md. Amirul Islam, Eunüs S. Ali, Siddhartha K. Mishra, Muhammad Torequl Islam, Mohammad S. Mubarak, Luciano da Silva Lopes, Ana Amélia de Carvalho Melo-CavalcanteAbstractEpilepsy is a neurological disease affecting people of all ages worldwide. Side ef...
Source: Biomedicine and Pharmacotherapy - July 29, 2018 Category: Drugs & Pharmacology Source Type: research

Ursolic acid loaded intra nasal nano lipid vesicles for brain tumour: Formulation, optimization, in-vivo brain/plasma distribution study and histopathological assessment
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Karishma Khan, Mohd Aqil, Syed Sarim Imam, Abdul Ahad, Thasleem Moolakkadath, Yasmin Sultana, Mohd MujeebAbstractThe aim was to formulate an optimized ursolic acid (UA) loaded lipid vesicle using formulation by design approach (FbD) for improving the drug targeting by nasal route for brain tumor. Three factors were evaluated at three different levels using anethole (terpene) (A), ethanol (B) and phospholipid90 G (C) as independent variables and their individual and combined effects were observed for PDI (Y1), vesicle size (...
Source: Biomedicine and Pharmacotherapy - July 28, 2018 Category: Drugs & Pharmacology Source Type: research

Intestinal absorption mechanisms of araloside A in situ single-pass intestinal perfusion and in vitro Caco-2 cell model
In this study, the absorption mechanisms of araloside A were investigated in a Caco-2 cell monolayer and in a single-pass intestinal perfusion in situ model with Sprague-Dawley rats. The effects of basic parameters, such as compound concentration, time, temperature, paracellular pathway, different intestinal segments were analyzed, and the susceptibility of araloside A absorption process to treatment with various inhibitors, such as the P-gp inhibitor verapamil, the multidrug resistance protein2 inhibitors (MRP2) MK571 and indomethacin, the breast cancer resistance protein (BCRP) inhibitors Ko143 and reserpine, and endocyt...
Source: Biomedicine and Pharmacotherapy - July 27, 2018 Category: Drugs & Pharmacology Source Type: research

Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Lijun Yang, Kaiyan Sun, Jie Chu, Yunhui Qu, Xue Zhao, Huiqing Yin, Liang Ming, Junhu Wan, Fucheng HeAbstractAims:Recent research showed that Long non-protein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in the course of tumor formation. The present study was aimed to explore its role in esophageal squamous cell carcinoma (ESCC).Main methodsQuantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to examine the expression levels of FTH1P3, mRNA SP1 and NF-kB in ESCC samples a...
Source: Biomedicine and Pharmacotherapy - July 27, 2018 Category: Drugs & Pharmacology Source Type: research

β-elemene against Burkitt’s lymphoma via activation of PUMA mediated apoptotic pathway
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Tonglin Hu, Yu GaoAbstractBurkitt’s lymphoma is a type of highly aggressive Non-Hodgkin's lymphoma. Although advanced Burkitt’s lymphoma is responsive to high-intensity chemotherapy regimens, increasing systemic toxicity, tumor recurrence and metastasis significantly reduce the patient survival. Thus, it is important to investigate novel antitumor agents with safety and effectiveness. β-elemene shows anti-proliferative effect on cancer cells by triggering apoptosis through regulating several molecular signaling pa...
Source: Biomedicine and Pharmacotherapy - July 27, 2018 Category: Drugs & Pharmacology Source Type: research

Phytochemicals, withaferin A and carnosol, overcome pancreatic cancer stem cells as c-Met inhibitors
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Shima Aliebrahimi, Shideh Montasser Kouhsari, Seyed Shahriar Arab, Amir Shadboorestan, Seyed Nasser OstadAbstractReceptor tyrosine kinases (RTKs) are pharmaceutically attractive targets due to their fundamental role in tumor formation. The hallmark of pancreatic cancer is its high mortality rate attributed to the existence of cancer stem cell (CSC) subpopulations which result in therapy resistance and recurrence. c-Met is a known pancreatic CSC marker that belongs to the family of RTKs. To surmount the hurdles related to ligand-in...
Source: Biomedicine and Pharmacotherapy - July 26, 2018 Category: Drugs & Pharmacology Source Type: research

In vitro and in vivo anticancer efficacy potential of Quercetin loaded polymeric nanoparticles
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Ruma Baksi, Devendra Pratap Singh, Swapnil P. Borse, Rita Rana, Vipin Sharma, Manish NivsarkarAbstractQuercetin (QCT) is a flavonoid, abundantly present in plants and has gained considerable interest for its antioxidant property and chemo preventive activity. Bioavailability of QCT is very low due to its poor aqueous solubility and instability. Researchers are working on the application of nanotechnology to target chemotherapeutic drugs to the tumour site. The aim of the present study was to develop quercetin loaded chitosan nanop...
Source: Biomedicine and Pharmacotherapy - July 25, 2018 Category: Drugs & Pharmacology Source Type: research

Long noncoding RNA OIP5-AS1 accelerates CDK14 expression to promote osteosarcoma tumorigenesis via targeting miR-223
In conclusion, our study found the critical regulation of OIP5-AS1/miR-223/CDK14 axis on osteosarcoma tumorigenesis, indicating the tumor promoting role of OIP5-AS1 for osteosarcoma.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Histamine H1 type receptor antagonist loratadine ameliorates oxidized LDL induced endothelial dysfunction
In this study, we revealed that loratadine, a histamine H1 type receptor specific antagonist, possesses a protective effect by relieving ox-LDL-induced endothelial inflammation. Treatment of endothelial cells with ox-LDL induces expression of the H1 receptor. The presence of loratadine in endothelial culture efficiently suppressed ox-LDL-induced attachment of monocytes to endothelial cells, production of ROS and vascular adhesion molecules, and induction cytokines including VCAM-1, E-selectin, TNF-α, IL-6 and IL-8. Mechanistically, we show that loratadine potently blocks ox-LDL-induced JNK activation as well as the A...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Long non-coding RNA NORAD upregulate SIP1 expression to promote cell proliferation and invasion in cervical cancer
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Huican Huo, Jun Tian, Ruyi Wang, Yanyun Li, Changping Qu, Ning WangAbstractLong non-coding RNAs (lncRNAs) play important roles in tumor progression. Recently, increasing evidence showed that lncRNA NORAD could be used as an important regulator in tumor progression. However, the roles and underlying mechanism of NORAD in cervical cancer (CC) remain unclear. In the present study, we found that NORAD expression was significantly up-regulated in CC tissues and cell lines. High NORAD expression was correlated with advanced FIGO stage, ...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Novel docetaxel chitosan-coated PLGA/PCL nanoparticles with magnified cytotoxicity and bioavailability
In conclusion, CS-decorated PLGA NPs are a considerable DTX-delivery carrier with magnificent antitumor efficacy.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Prunetin inhibits lipopolysaccharide-induced inflammatory cytokine production and MUC5AC expression by inactivating the TLR4/MyD88 pathway in human nasal epithelial cells
In conclusion, prunetin inhibited LPS-induced inflammatory cytokine production and MUC5 AC expression and secretion by inactivating the TLR4/MyD88 pathway in human nasal epithelial cells. These results suggested that prunetin might be a useful agent in the treatment of AR.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

ADMA, homocysteine and redox status improvement affected by 7-nitroindazole in spontaneously hypertensive rats
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Ima Dovinová, Eva Hrabárová, Eugene Jansen, Miroslava Kvandová, Miroslava Majzúnová, Andrea Berenyiová, Miroslav BarančíkAbstractInhibition of nitric oxide (NO) production can influence blood pressure regulation and increase hypertension. Asymmetric dimethylarginine, ADMA, an analogue of L-arginine, can inhibit NO synthesis, impair endothelial function, and is a risk marker of cardiovascular diseases. Homocysteine (Hcy) level affects oxidative stress production of reactive o...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Neuroprotective role of fucoxanthin against cerebral ischemic/reperfusion injury through activation of Nrf2/HO-1 signaling
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Lixun Hu, Weimei Chen, Feng Tian, Chao Yuan, Hongjuan Wang, Hongmei YueAbstractIn the present study, an attempt was made to determine whether administration of fucoxanthin could attenuate cerebral ischemic/reperfusion (I/R) injury and its possible mechanisms using an in vivo middle cerebral artery occlusion (MCAO) model and an in vitro oxygen-glucose deprivation and reoxygenation (OGD/R) model. Fucoxanthin was intragastrically administrated in different doses (30 mg/kg, 60 mg/kg, and 90 mg/kg, respectively) to...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

The potential hepatoprotective effect of royal jelly against cadmium chloride-induced hepatotoxicity in mice is mediated by suppression of oxidative stress and upregulation of Nrf2 expression
This study was aimed at investigating the possible protective mechanism of royal jelly (RJ) against hepatotoxicity induced by cadmium chloride (CdCl2). The study included four groups: the control group received saline (0.9% sodium chloride), CdCl2 group received 6.5 mg/kg CdCl2 for seven days, RJ group received 85 mg/kg standardized RJ containing 6% 10-hydroxy-2-decenoic acid equivalent to 250 mg crude RJ, and finally, the fourth group received RJ 2 h before CdCl2 injection daily for 7 days. Oxidant/antioxidant markers of liver function estimation and histopathology were determined. The results revealed that RJ sig...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Novel endodontic sealers induced satisfactory tissue response in mice
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Lea Assed Bezerra da Silva, Amanda Silva Bertasso, Carolina Maschietto Pucinelli, Raquel Assed Bezerra da Silva, Katharina Morant Holanda de Oliveira, Manoel Damião Sousa-Neto, Alberto ConsolaroAbstractObjectivesThe aim of this study was to evaluate the subcutaneous response induced by Roeko Guttaflow2 (RG), Sealapex Xpress (SX), AH Plus (AHP) sealers.Methods100 BALB/c mice received implants in the subcutaneous tissue with the tested materials (10 animals per period for each evaluated sealer) and were evaluated after differ...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Xuebijing injection induces anti-inflammatory-like effects and downregulates the expression of TLR4 and NF-κB in lung injury caused by dichlorvos poisoning
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Fei He, Jun Wang, Yao Liu, Xiaojing Wang, Nan Cai, Chao Wu, Qingling GaoAbstractBackgroundThe mechanism in lung injury caused by acute organophosphate pesticide poisoning (AOPP) and an effective treatment remains unclear. We aim to clarify how the inflammatory lung injury caused by AOPP might be modulated by Xuebijing (XBJ) injection.MethodsAOPP-induced lung injury model was induced by dichlorvos (DDVP) subcutaneous administration in rats and XBJ injection was administered by intraperitoneal injection after DDVP challenge. The eff...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

MAPK8 mediates resistance to temozolomide and apoptosis of glioblastoma cells through MAPK signaling pathway
ConclusionMAPK8 promoted the resistance to TMZ, accelerated cell proliferation and inhibited the apoptosis of glioblastoma cells via activating MAPK signaling pathway. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Withaferin-A attenuates multiple low doses of Streptozotocin (MLD-STZ) induced type 1 diabetes
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Sravani Tekula, Amit Khurana, Pratibha Anchi, Chandraiah GoduguAbstractType 1 diabetes mellitus (T1DM) is one of the major metabolic disorders with life-long dependence on insulin. The present study was designed to evaluate the antioxidant and anti-diabetic potential of Withaferin A (WA), the active constituent of Withania somnifera in multiple low doses of Streptozotocin (MLD-STZ) induced T1DM. STZ (40 mg/Kg) was administered intraperitoneally (i.p.) for 5 consecutive days to male Swiss albino mice to induce T1DM. Mice wer...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Theabrownin suppresses in vitro osteoclastogenesis and prevents bone loss in ovariectomized rats
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Titi Liu, Zemin Xiang, Fei Chen, Dan Yin, Yewei Huang, Jing Xu, Lihong Hu, Huanhuan Xu, Xuanjun Wang, Jun ShengAbstractDrinking tea exhibits beneficial effects on bone health and may protect against osteoporosis, particularly in postmenopausal women. Theabrownin (TB) is the main component responsible for the biological activities of Pu-erh tea, but whether it possesses anti-osteoporotic potential remains unknown. Here we investigated the in vitro and in vivo anti-osteoporotic effects of TB in the RAW 264.7 cell line and ovariectom...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Down-regulation of Rictor enhances cell sensitivity to PI3K inhibitor LY294002 by blocking mTORC2-medicated phosphorylation of Akt/PRAS40 in esophageal squamous cell carcinoma
The objective of this study is to evaluate the effects of Rictor knockdown on cell sensitivity to PI3K inhibitor LY294002 in ESCC cells and ESCC xenografts as well as its mechanisms. We found LY294002 obviously restrained cell proliferation in dose-dependent and time-dependent manners by inhibiting PI3K/Akt/mTOR/p70S6K signaling pathway, whereas triggered mTORC2-medicated phosphorylation of Akt (Ser473)/PRAS40 (Thr246) in ECa109 and EC9706 cells. Stable knockdown of Rictor by shRNA enhanced the inhibitory effects of LY294002 on cell proliferative, migration and colony formation, as well as promoted its effects on cell cycl...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

MiR-1294 confers cisplatin resistance in ovarian Cancer cells by targeting IGF1R
ConclusionOur results indicated that epigenetic regulation of IGF1R via miR-1294 was essential for cisplatin resistance in OC and provide a new avenue for OC treatment. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Exendin-4 inhibits glioma cell migration, invasion and epithelial-to-mesenchymal transition through GLP-1R/sirt3 pathway
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Zhi-Jun Nie, Yin-Gang Zhang, Yan-Hai Chang, Quan-Yi Li, Yue-Lin ZhangAbstractGLP-1 analogue exendin-4, a glucagon-like peptide 1 receptor (GLP-1R) agonist which shares 53% sequence with GLP-1, plays an essential role in human tumors. However, the function and mechanisms underlying the effects of exendin-4 on glioma cell migration, invasion and epithelial-to-mesenchymal transition are still obscure. Firstly, we demonstrated that GLP-1R was expressed in all glioma cell lines including U87, U251, U373 and A172. Exendin-4 treatment in...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

MiR-92a promotes tumorigenesis of colorectal cancer, a transcriptomic and functional based study
In conclusion, miR-92a acts as an oncomir and directly targets Wnt/β-catenin, PTEN/Akt/FoxO, and BMP/Smads related genes, thus participates in CRC progression.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Anti-hypertrophy effect of atorvastatin on myocardium depends on AMPK activation-induced miR-143-3p suppression via Foxo1
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Bo Yu, Dongna Liu, Hongli Zhang, Di Xie, Wei Nie, Kaiyao Shi, Ping YangAbstractLeft ventricular hypertrophy (LVH) is a pathological characteristic shared by distinct heart disorders. Atorvastatin is employed as a lipid lowering agent and its heart protection effect has been recently reported as well. Thus, the current study attempted to validate the anti-hypertrophy effect of atorvastatin as well as the associated mechanism. Hypertrophic feature was induced in rats using transverse aortic constriction (TAC) method and in cardiomyo...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Silencing of cytoskeleton-associated protein 2 represses cell proliferation and induces cell cycle arrest and cell apoptosis in osteosarcoma cells
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Shuwei Zhang, Yi Wang, Shuzhen Chen, Jingfeng LiAbstractOsteosarcoma is the most common primary bone malignancy, mainly occurring in children and adolescents. Cytoskeleton-associated protein 2 (CKAP2), which plays important roles in cell proliferation, has been reported to be overexpressed in diverse human cancers. In the present study, we aimed at exploring the expression and functions of CKAP2 in osteosarcoma. The mRNA and protein expression of CKAP2 was analyzed on collected osteosarcoma and control bone cyst tissues. The resul...
Source: Biomedicine and Pharmacotherapy - July 24, 2018 Category: Drugs & Pharmacology Source Type: research

Apocynin inhibited NLRP3/XIAP signalling to alleviate renal fibrotic injury in rat diabetic nephropathy
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Rui Xin, Xuhong Sun, Ziying Wang, Wendan Yuan, Weidong Jiang, Lin Wang, Yanxiao Xiang, Hongqin Zhang, Xiaoyan Li, Yun Hou, Wangnan Sun, Pengchao DuAbstractAimsIn this animal study, we tried to test the hypothesis that apocynin could play an anti-inflammation role by inhibiting NLRP3/X-linked inhibitor of apoptosis protein (XIAP) signalling and have an effect on antifibrosis in rats with diabetic nephropathy.Main methodsDiabetic nephropathy rats were induced by tail-vein injection of streptozotocin at 60 mg/kg body weight in sodi...
Source: Biomedicine and Pharmacotherapy - July 22, 2018 Category: Drugs & Pharmacology Source Type: research

Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity
In this study, the effect and mechanism of YGL in reducing liver fibrosis was demonstrated in vivo. Our results showed that liver fibrosis biomarkers collagen IV (Col IV), type III precollagen (PCIII), hyaluronuc acid (HA) and laminin (LN), were increased after CCl4 treatment and decreased by YGL. Among the liver fibrosis indicators, α-smooth muscle actin (α-SMA) was decreased by YGL in the CCl4-treated rats, while MMP2 and MMP9 was upregulated followed by TIMP1 downregulation. Proteins involved in liver fibrosis such as p-Smad2, p-Smad3 and Smad4 were down-regulated, while Smad7 protein was up-regulated by YGL...
Source: Biomedicine and Pharmacotherapy - July 22, 2018 Category: Drugs & Pharmacology Source Type: research

Synergistic antitumour effects of triptolide plus gemcitabine in bladder cancer
ConclusionThe combination of GEM plus TPL showed significantly enhanced anticancer effects compared to those of GEM or TPL alone. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 21, 2018 Category: Drugs & Pharmacology Source Type: research

Ximenia americana heteropolysaccharides ameliorate inflammation and visceral hypernociception in murine caerulein-induced acute pancreatitis: Involvement of CB2 receptors
ConclusionsTPL-Xa contains heteropolysaccharides that inhibit inflammation and hypernociception in the experimental model of caerulein-induced AP, by a mechanism involving type CB2 receptors.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 21, 2018 Category: Drugs & Pharmacology Source Type: research

The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Jinchun Wang, Lei Wang, Jie Zhou, Aiping Qin, Zhujing ChenAbstractThe present study was aimed to elucidate the pharmacological effect of Formononetin (FMN) treatment on STZ-induced diabetic cognitive dysfunction. The diabetic model was induced by an intraperitoneally injection of 180 mg/kg STZ. The animals were randomly divided into five groups: control group, streptozocin (STZ, 180 mg/kg) group, STZ + metformin (Met, 200 mg/kg) group, STZ + FMN (25 mg/kg) group, STZ + FMN (50 mg/kg) group. The mice were intr...
Source: Biomedicine and Pharmacotherapy - July 21, 2018 Category: Drugs & Pharmacology Source Type: research

Sevoflurane reduced functional connectivity of excitatory neurons in prefrontal cortex during working memory performance of aged rats
This study aimed to evaluate the effects of sevoflurane on working memory of rats and to characterize functional connectivity between excitatory neurons in the prefrontal cortex (PFC) during working memory performance.Methods18-month-old and 3-month-old male SD rats were implanted with a multielectrode array in the PFC. After recovery from the surgery, the aged and adult rats were divided into sevoflurane group and control group, respectively. Rats in sevoflurane group were exposed to 1 minimum alveolar concentration (MAC) or 1.5 MAC sevoflurane for two hours. At day1, 3, and 7 after exposure, the effects of sevoflurane an...
Source: Biomedicine and Pharmacotherapy - July 21, 2018 Category: Drugs & Pharmacology Source Type: research

Tauroursodeoxycholic acid alleviates hepatic ischemia reperfusion injury by suppressing the function of Kupffer cells in mice
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Xuesong Xu, Menghao Wang, Jin-zheng Li, Si-Dong Wei, Hao Wu, Xing Lai, Ding Cao, Zhi-bing Ou, Jianping GongAbstractThe aim of this study is to investigate the protective effect and the mechanism of tauroursodeoxycholic acid (TUDCA) against hepatic ischemia reperfusion (IR) injury. Male Balb/c mice were intraperitoneally injected with tauroursodeoxycholic acid (400 mg/kg) or saline solution, once per day for 3 days before surgery, and then the model of hepatic I/R injury was established. Blood and liver samples were collected fro...
Source: Biomedicine and Pharmacotherapy - July 21, 2018 Category: Drugs & Pharmacology Source Type: research

Xanthohumol induces apoptosis via caspase activation, regulation of Bcl-2, and inhibition of PI3K/Akt/mTOR-kinase in human gastric cancer cells
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Dongli Guo, Baogui Zhang, Shiqi Liu, Meng JinAbstractWe assessed the effect of xanthohumol (XN) on gastric cancer (GC) in vitro and in vivo. XN reduced the viability of SGC-7901, SNU216, and SNU668 cells, but not GES-1 non-tumorigenic human gastric epithelial cells. XN induced apoptosis in SGC-7901 cells in a concentration-dependent manner by enhancing the numbers of late and early apoptotic cells. XN also downregulated the anti-apoptotic proteins Bcl-XL and Bcl-2 and upregulated the pro-apoptotic proteins Bax, Bid, PARP, and casp...
Source: Biomedicine and Pharmacotherapy - July 21, 2018 Category: Drugs & Pharmacology Source Type: research

Determination of gliclazide minimum concentration in type 2 diabetes mellitus patients
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Andrzej Czyrski, Matylda Resztak, Tadeusz HermannAbstractType 2 diabetes mellitus is a worldwide health problem. Many drugs can be used in its treatment. One of them is gliclazide – the sulfonylurea derivative. It is dosed in modified release tablets. The study aimed to determine the minimum steady-state concentration of gliclazide at patients taking modified release tablets. Fasting plasma glucose, insulin level, and glycated hemoglobin were also assayed in this study. Ten patients of the primary care physician clinic took ...
Source: Biomedicine and Pharmacotherapy - July 21, 2018 Category: Drugs & Pharmacology Source Type: research

LncRNA THOR acts as a retinoblastoma promoter through enhancing the combination of c-myc mRNA and IGF2BP1 protein
In conclusion, this study makes clear that lncRNA THOR is up-regulated in retinoblastoma, and its over-expression significantly enhances the malignant phenotype transformation of retinoblastoma cells through up-regulating c-myc expression via enhancing its combination with TGF2BP1 protein. Overall, our study illustrates that lncRNA THOR/c-myc molecular cascade might be another potent target for retinoblastoma treatment. (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 20, 2018 Category: Drugs & Pharmacology Source Type: research

LncRNA FOXD2-AS1 regulates chondrocyte proliferation in osteoarthritis by acting as a sponge of miR-206 to modulate CCND1 expression
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Lei Cao, Yang Wang, Qiugen Wang, Jianhua HuangAbstractRecently, accumulating evidence demonstrated that the long non-coding RNAs (lncRNAs) play important roles in osteoarthritis (OA) progression. However, the role of lncRNA FOXD2-AS1 on OA is still unclear. In the present study, qRT-PCR showed that expression of FOXD2-AS1 and Cyclin D1 (CCND1) was upregulated in OA cartilage tissues, while miR-206 expression was significantly decreased. CCK-8 and colony formation assays showed that FOXD2-AS1 could promote chondrocytes viability. F...
Source: Biomedicine and Pharmacotherapy - July 20, 2018 Category: Drugs & Pharmacology Source Type: research

Puerarin promoted proliferation and differentiation of dopamine-producing cells in Parkinson’s animal models
ConclusionThe results suggest that PR promote DA neuron survival, proliferation and differentiation.Graphical abstract (Source: Biomedicine and Pharmacotherapy)
Source: Biomedicine and Pharmacotherapy - July 20, 2018 Category: Drugs & Pharmacology Source Type: research

Antioxidant and apoptotic effects of Callistemon lanceolatus leaves and their compounds against human cancer cells
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Kamal Ahmad, Zubair Bin Hafeez, Abdul Roof Bhat, Moshahid Alam Rizvi, Sonu C. Thakur, Amir Azam, Fareeda AtharAbstractCallistemon lanceolatus (Myrtaceae) has been utilized in folk medicine and its pharmacological properties are widely studied. Phytochemicals are effectively recognized as bases of pharmacologically potent drugs for the development of anticancer therapeutics. The free radical scavenging potential of numerous extracts of C. lanceolatus leaves, Hexane leaf extract (HLE), Chloroform leaf extract (CLE), Ethyl acetate le...
Source: Biomedicine and Pharmacotherapy - July 20, 2018 Category: Drugs & Pharmacology Source Type: research

Royal jelly increases peripheral circulation by inducing vasorelaxation through nitric oxide production under healthy conditions
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Yaoyue Liang, Satomi Kagota, Kana Maruyama, Yuri Oonishi, Shino Miyauchi-Wakuda, Yoshihiko Ito, Shizuo Yamada, Kazumasa ShinozukaAbstractAimsRoyal jelly (RJ) has a variety of reported biological activities, including vasorelaxation and blood pressure–lowering effects. Although functional foods are positively used for health, the effects of RJ on the cardiovascular system in healthy individuals have not been well studied. Therefore, we investigated the mechanisms underlying the vasorelaxation effects of RJ in healthy control ...
Source: Biomedicine and Pharmacotherapy - July 20, 2018 Category: Drugs & Pharmacology Source Type: research

Protective effect of Rotula aquatica Lour against gentamicin induced oxidative stress and nephrotoxicity in Wistar rats
Publication date: October 2018Source: Biomedicine & Pharmacotherapy, Volume 106Author(s): Vysakh A., Abhilash S., Jayesh Kuriakose, Sebastian Jose Midhun, Mathew Jyothis, M.S. LathaAbstractGentamicin is an aminoglycoside antibiotic widely used for the treatment of life-threatening infections caused by Gram-negative bacteria. The use of gentamicin was limited due to its ototoxic and nephrotoxic adverse effects. The current study was designed to evaluate the protective effect of ethyl acetate fraction from Rotula aquatica (EFRA) against gentamicin induced nephrotoxicity. The antioxidant enzymes status, lipid peroxidation...
Source: Biomedicine and Pharmacotherapy - July 19, 2018 Category: Drugs & Pharmacology Source Type: research

Clerodendrum volubile inhibits key enzymes linked to type 2 diabetes but induces cytotoxicity in human embryonic kidney (HEK293) cells via exacerbated oxidative stress and proinflammation
In this study, the antidiabetic properties of the dichloromethane (DCM) fraction of C. volubile leaves were investigated in vitro. Its cytotoxic effect and mechanism of toxicity were also investigated in Human Embryonic Kidney (HEK293) cells. The fraction was subjected to in vitro antioxidant assays using the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) scavenging and Ferric reducing antioxidant power (FRAP) protocols. Its enzyme-inhibitory properties were investigated on α-glucosidase and α-amylase activities. Gas Chromatography Mass Spectroscopy (GCMS) and Fourier Transform Infrared (FTIR) spectroscopic analys...
Source: Biomedicine and Pharmacotherapy - July 18, 2018 Category: Drugs & Pharmacology Source Type: research