Fc Receptor-Like 1 as a Promising Target for Immunotherapeutic Interventions of B-Cell-Related Disorders.
Conclusions: These results suggest that FCRL1 can play a key role in the activation of human B-cell responses and has the potential to serve as a target for immunotherapy of FCRL1 positive B-cell-related disorders. PMID: 31798301 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - December 6, 2019 Category: General Medicine Authors: Yousefi Z, Sharifzadeh S, Yar-Ahmadi V, Andalib A, Eskandari N Tags: Biomark Insights Source Type: research

Serum Potassium, Sodium, and Chloride Levels in Sickle Cell Disease Patients and Healthy Controls: A Case-Control Study at Korle-Bu Teaching Hospital, Accra.
This study evaluated the serum levels of sodium, potassium, and chloride in patients with SCD. The study was a case-control cross-sectional study involving 120 SCD patients in the steady state and 48 'healthy' controls. The SCD patients were made up of 69 HbSS patients and 41 HbSC patients. Serum electrolyte levels (Na+, K+, and Cl-) were measured using a Flame Atomic Absorption Spectrometer (Variant 240FS; Varian Australia Pty Ltd). Serum sodium levels were significantly lower in the sickle cell patients, compared with their 'healthy' counterparts (P = .0001). Although the study found significantly higher se...
Source: Biomarker Insights - September 18, 2019 Category: General Medicine Authors: Antwi-Boasiako C, Kusi-Mensah YA, Hayfron-Benjamin C, Aryee R, Dankwah GB, Abla KL, Owusu Darkwa E, Botchway FA, Sampene-Donkor E Tags: Biomark Insights Source Type: research

Serum Metabolomic Profiles of Rheumatoid Arthritis Patients With Acute-Onset Diffuse Interstitial Lung Disease.
Conclusion: It was demonstrated that metabolomic profiling was useful to generate better biomarkers in AoDILD. PMID: 31488947 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - September 7, 2019 Category: General Medicine Authors: Furukawa H, Oka S, Shimada K, Hashimoto A, Komiya A, Matsui T, Fukui N, Tohma S Tags: Biomark Insights Source Type: research

Circulatory Levels of RANKL, OPG, and Oxidative Stress Markers in Postmenopausal Women With Normal or Low Bone Mineral Density.
Conclusions: These data provide insights into the possible roles of OPG, RANKL, and oxidative stress in the pathogenesis of postmenopausal osteoporosis. However, the lack of association between these markers and BMD indicates that osteoporosis is complex and multivariate. PMID: 31452599 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - August 29, 2019 Category: General Medicine Authors: Azizieh FY, Shehab D, Jarallah KA, Gupta R, Raghupathy R Tags: Biomark Insights Source Type: research

A Comparison of Death Domain-Associated Protein 6 in Different Endometrial Carcinomas Histotypes.
Conclusions: We demonstrate that loss of nuclear DAXX is present in low-grade endometrial carcinomas and the differentiated components in UEC/DDEC, but not in high-grade ones, suggesting DAXX's role in tumor progression and its potential as a therapeutic target in high-grade endometrial carcinomas. PMID: 31384126 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - August 8, 2019 Category: General Medicine Authors: Jin C, Hacking S, Komforti MK, Nasim M Tags: Biomark Insights Source Type: research

Prognostic Value of MMP-9 -1562 C/T Gene Polymorphism in Patients With Sepsis.
Conclusions: We concluded that there was no association between the SNP MMP9 -1562 C/T and sepsis or between the SNP MMP9 -1562 C/T and sepsis mortality in the Northeastern Colombian septic patient cohort. Further research is needed to clarify the correlation among sepsis, genetic factors with allele T and MMP-9 plasma concentration. PMID: 31205414 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - June 19, 2019 Category: General Medicine Authors: Bermúdez-Mejía C, Torres-Cordón MF, Becerra-Bayona S, Páez CM, Vargas CI, Cárdenas ME, Serrano SE, Baquero I, Martínez-Vega R, Schulz R, Ilarraza R, Pazin Filho A, Torres-Dueñas D Tags: Biomark Insights Source Type: research

Neutrophil to Lymphocyte Ratio is a Predictive Factor of Malignant Potential for Intraductal Papillary Mucinous Neoplasms of the pancreas.
Abstract Intraductal papillary mucinous neoplasms (IPMNs) are cystic neoplasms with the potential for progression to pancreatic cancer. Accurate prediction of the malignant potential is challenging and a proper treatment strategy has not been well established. Preoperative neutrophil-to-lymphocyte ratio (NLR) is a biomarker of the malignant potential in patients with several types of malignancy. We explored malignant potential in patients with IPMN. The present study included 56 patients aged of 73 ± 9 years (mean ± standard deviation) who underwent curative resection for IPMN fr...
Source: Biomarker Insights - June 19, 2019 Category: General Medicine Authors: Ohno R, Kawamoto R, Kanamoto M, Watanabe J, Fujii M, Ohtani H, Harada M, Kumagi T, Kawasaki H Tags: Biomark Insights Source Type: research

Cerebral Expression of Glial Fibrillary Acidic Protein, Ubiquitin Carboxy-Terminal Hydrolase-L1, and Matrix Metalloproteinase 9 After Traumatic Brain Injury and Secondary Brain Insults in Rats.
Abstract Glial fibrillary acidic protein (GFAP), ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1), and matrix metalloproteinase 9 (MMP-9) are potential biomarkers of traumatic brain injury (TBI) but also of secondary insults to the brain. The aim of this study was to describe the cerebral distribution of GFAP, UCH-L1, and MMP-9 in a rat model of diffuse TBI associated with standardized hypoxia-hypotension (HH). Adult male Sprague-Dawley rats were allocated to Sham (n = 10), TBI (n = 10), HH (n = 10), and TBI+HH (n = 10) groups. After 4 hours, brains were rapi...
Source: Biomarker Insights - June 19, 2019 Category: General Medicine Authors: Mrozek S, Delamarre L, Capilla F, Al-Saati T, Fourcade O, Constantin JM, Geeraerts T Tags: Biomark Insights Source Type: research

Oncogenic Roles and Inhibitors of DNMT1, DNMT3A, and DNMT3B in Acute Myeloid Leukaemia.
Abstract Epigenetic alteration has been proposed to give rise to numerous classic hallmarks of cancer. Impaired DNA methylation plays a central role in the onset and progression of several types of malignancies, and DNA methylation is mediated by DNA methyltransferases (DNMTs) consisting of DNMT1, DNMT3A, and DNMT3B. DNMTs are frequently implicated in the pathogenesis and aggressiveness of acute myeloid leukaemia (AML) patients. In this review, we describe and discuss the oncogenic roles of DNMT1, DNMT3A, and DNMT3B in AML. The clinical response predictive roles of DNMTs in clinical trials utilising hypomethylatin...
Source: Biomarker Insights - May 22, 2019 Category: General Medicine Authors: Wong KK, Lawrie CH, Green TM Tags: Biomark Insights Source Type: research

Chemotherapy-Induced Hair Loss: The Use of Biomarkers for Predicting Alopecic Severity and Treatment Efficacy.
This article describes the potential utility of a combination of biomarkers in assessing drug-induced alopecia and the protective potential of existing or new treatments. A greater understanding of the precise mechanisms of anti-CIA therapies through biomarker analysis would enhance the rationale, use, and development of such treatments. PMID: 31037027 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - May 2, 2019 Category: General Medicine Authors: Haslam IS, Smart E Tags: Biomark Insights Source Type: research

Identification of Urinary CD44 and Prosaposin as Specific Biomarkers of Urinary Tract Infections in Children With Neurogenic Bladders.
Conclusion: Urinary CD44 and prosaposin are candidate markers that may assist with the diagnosis of UTI in CIC-dependent children. PMID: 30906192 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - March 26, 2019 Category: General Medicine Authors: Forster CS, Haffey WD, Bennett M, Greis KD, Devarajan P Tags: Biomark Insights Source Type: research

Methylenetetrahydrofolate Reductase C677T Gene Polymorphism as Risk Factor for Psoriasis in Saudis.
Abstract Methylenetetrahydrofolate reductase (MTHFR) has been linked with the etiopathogenesis of psoriasis with inconsistent results. Methylenetetrahydrofolate reductase C677T polymorphism was evaluated in 106 Saudi psoriasis vulgaris patients and 280 matched healthy controls using PCR-RFLP (restriction fragment length plymorphism) technique. The cardiovascular risk factors were also compared in cases and controls. Allele T and genotypes CT and TT were found to be increased while allele C and genotype CC significantly decreased in psoriasis patients as compared with controls (P 
Source: Biomarker Insights - March 6, 2019 Category: General Medicine Authors: Huraib GB, Harthi FA, Arfin M, Khlaiwi AA, Rizvi S, Al-Asmari A Tags: Biomark Insights Source Type: research

The Possibility of Systematic Research Fraud Targeting Under-Studied Human Genes: Causes, Consequences, and Potential Solutions.
We describe how fraudulent papers may be produced in series by paper mills using what we have described as a 'theme and variations' model, which could also be considered a form of salami slicing. We describe features of these single-gene knockdown publications that may allow them to evade detection by journal editors, peer reviewers, and readers. We then propose a number of approaches to facilitate their detection, including improved awareness of the features of publications constructed in series, broader requirements to post submitted manuscripts to preprint servers, and the use of semi-automated literature screening tool...
Source: Biomarker Insights - February 21, 2019 Category: General Medicine Authors: Byrne JA, Grima N, Capes-Davis A, Labbé C Tags: Biomark Insights Source Type: research

Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitorTM Assay.
Abstract The standard of care in oncology has been genomic profiling of tumor tissue biopsies for the treatment and management of disease, which can prove to be quite challenging in terms of cost, invasiveness of procedure, and potential risk for the patient. As the number of available drugs in oncology continues to increase, so too does the demand for technologies and testing applications that can identify genomic alterations targetable by these new therapies. Liquid biopsies that use a blood draw from the diseased patient may offset the many disadvantages of the invasive procedure. However, as with any new techn...
Source: Biomarker Insights - February 14, 2019 Category: General Medicine Authors: Sisson BA, Uvalic J, Kelly K, Selvam P, Hesse AN, Ananda G, Chandok H, Bergeron D, Holinka L, Reddi HV Tags: Biomark Insights Source Type: research

The Fraction Exhaled Nitric Oxide as a Biomarker of Asthma Control.
Conclusions: FeNO level could be helpful in determining asthma control as>30 ppb was associated with uncontrolled asthma. PMID: 30728712 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - February 10, 2019 Category: General Medicine Authors: de Abreu FC, da Silva Júnior JLR, Rabahi MF Tags: Biomark Insights Source Type: research

Peripheral Biomarker for Vascular Disorders.
Abstract Atherosclerosis is the underlying cause of most myocardial infarction (MI) and ischaemic stroke episodes. An early sign of atherosclerosis is hypertrophy of the arterial wall. It is known that increased intima media thickness (IMT) is a non-invasive marker of arterial wall alteration, which can easily be assessed in the carotid arteries by high-resolution B-mode ultrasound. Similarly, the other key element of MI and ischaemic strokes is the N-methyl-D-aspartate (NMDA) receptor which is an ionotropic glutamate receptor that mediates the vast majority of excitatory neurotransmission in the brain. NMDA activ...
Source: Biomarker Insights - December 15, 2018 Category: General Medicine Authors: Shad KF, Luqman N, Simpson AM, Lal S Tags: Biomark Insights Source Type: research

Integration of Biomarkers Into a Signature Profile of Persistent Traumatic Brain Injury Involving Autoimmune Processes Following Water Hammer Injury From Repetitive Head Impacts.
Conclusions: An assessment of an individuals' predisposition to persistent TBI with delayed cognitive deficits and behavioral changes requires an understanding of host vulnerability (genetic factors and brain structure) and external stressors (force and torque of impact as well as repetitive head injury and time interval between impacts). An algorithm that has utility in predicting vulnerability to TBI will include qualitative and quantitative measures of the host factors weighted against post impact markers of neural injury. Implementation of the resulting "Signature" of vulnerability at early stages of injury w...
Source: Biomarker Insights - November 8, 2018 Category: General Medicine Authors: Kornguth S, Rutledge N Tags: Biomark Insights Source Type: research

MicroRNAs as Biomarkers of B-cell Lymphoma.
ie CH Abstract B-cell lymphomas represent a diverse group of neoplasms classified primarily by histopatholgy and are often challenging to accurately diagnose. Despite having been recognized less than 20 years ago, microRNAs (miRNAs) have emerged as one of the most promising class of cancer molecular biomarkers and are particularly attractive as they can be readily detected in formalin-fixed paraffin-embedded biopsy material and biological fluids such as blood. Many of the identified B-cell lymphoma miRNA biomarkers also play crucial regulatory roles in normal B-cell development. Below we consider the identi...
Source: Biomarker Insights - October 24, 2018 Category: General Medicine Authors: Solé C, Arnaiz E, Lawrie CH Tags: Biomark Insights Source Type: research

Identifying Reliable Diagnostic/Predictive Biomarkers for Rheumatoid Arthritis.
Conclusions: The data from this study suggest the use of HSPA6, MMP1, MMP13, and TNFSF10 gene expression profiles as potential diagnostic biomarkers. In addition, these gene profiles can help in predicting the therapeutic efficacy of MTX. PMID: 30262983 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - September 30, 2018 Category: General Medicine Authors: Shervington L, Darekar A, Shaikh M, Mathews R, Shervington A Tags: Biomark Insights Source Type: research

Cytokine Measurements for Diagnosing and Characterizing Leukemoid Reactions and Immunohistochemical Validation of a Granulocyte Colony-Stimulating Factor and CXCL8-Producing Renal Cell Carcinoma.
Conclusions: In suspected paraneoplastic leukocytosis, multiplex serum cytokine analyses may facilitate diagnosis and provide an understanding of the mechanisms for the reaction. In the index patient, combined G-CSF and CXCL8 protein expression by renal carcinoma cells was uniquely documented. A rapidly fatal course was detected in all 3 cases, congruent with the concept that autocrine/paracrine growth signaling in renal carcinoma cells may induce an aggressive tumor phenotype. Immune profiling studies could improve our understanding for possible targets when choosing therapies for patients with metastatic renal cell carci...
Source: Biomarker Insights - August 28, 2018 Category: General Medicine Authors: Åström M, Tajeddinn W, Karlsson MG, Linder O, Palmblad J, Lindblad P Tags: Biomark Insights Source Type: research

Is Procalcitonin Useful in Pediatric Critical Care Patients?
cia I Abstract This review examines the use of procalcitonin in different clinical situations in the pediatric patient, with special emphasis on those requiring intensive care. We review the latest articles on its potency as a biomarker in both infectious processes at diagnosis and on the response to treatment. PMID: 30093797 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - August 11, 2018 Category: General Medicine Authors: Bobillo-Perez S, Rodríguez-Fanjul J, Jordan Garcia I Tags: Biomark Insights Source Type: research

Development of a Novel Proteomic Risk-Classifier for Prognostication of Patients With Early-Stage Hormone Receptor-Positive Breast Cancer.
Abstract Use of proteomic strategies to identify a risk classifier that estimates probability of distant recurrence in early-stage hormone receptor (HR)-positive breast cancer is relevant to physiological cellular function and therefore to intrinsic tumor biology. We used a 298-sample retrospective training set to develop an immunohistochemistry-based novel risk classifier called CanAssist-Breast (CAB) which combines 5 prognostically relevant biomarkers and 3 clinico-pathological parameters to arrive at probability of distant recurrence within 5 years from diagnosis. Five selected biomarkers, namely, CD44, ...
Source: Biomarker Insights - August 8, 2018 Category: General Medicine Authors: Ramkumar C, Buturovic L, Malpani S, Kumar Attuluri A, Basavaraj C, Prakash C, Madhav L, Doval DC, Mehta A, Bakre MM Tags: Biomark Insights Source Type: research

Heart Rate Variability as a Biomarker for Predicting Stroke, Post-stroke Complications and Functionality.
Conclusions: Current research provides good evidence that HRV parameters may have utility as a biomarker for stroke and for post-stroke complications and/or functionality. Future research would benefit from the integration of non-linear, and novel parameters, the hybridisation of HRV parameters, and the expansion of the utilisation of predictive regression and hazard modelling. PMID: 30038486 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - July 25, 2018 Category: General Medicine Authors: Lees T, Shad-Kaneez F, Simpson AM, Nassif NT, Lin Y, Lal S Tags: Biomark Insights Source Type: research

Multiple Endocrine Neoplasia Syndromes from Genetic and Epigenetic Perspectives.
Abstract Multiple endocrine neoplasia (MEN) syndromes are infrequent inherited disorders in which more than one endocrine glands develop noncancerous (benign) or cancerous (malignant) tumors or grow excessively without forming tumors. There are 3 famous and well-known forms of MEN syndromes (MEN 1, MEN 2A, and MEN 2B) and a newly documented one (MEN4). These syndromes are infrequent and occurred in all ages and both men and women. Usually, germ line mutations that can be resulted in neoplastic transformation of anterior pituitary, parathyroid glands, and pancreatic islets in addition to gastrointestinal tract can ...
Source: Biomarker Insights - July 19, 2018 Category: General Medicine Authors: Khatami F, Tavangar SM Tags: Biomark Insights Source Type: research

Nattokinase: A Promising Alternative in Prevention and Treatment of Cardiovascular Diseases.
Abstract Cardiovascular disease (CVD) is the leading cause of death in the world and our approach to the control and management of CVD mortality is limited. Nattokinase (NK), the most active ingredient of natto, possesses a variety of favourable cardiovascular effects and the consumption of Natto has been linked to a reduction in CVD mortality. Recent research has demonstrated that NK has potent fibrinolytic activity, antihypertensive, anti-atherosclerotic, and lipid-lowering, antiplatelet, and neuroprotective effects. This review covers the major pharmacologic effects of NK with a focus on its clinical relevance ...
Source: Biomarker Insights - July 19, 2018 Category: General Medicine Authors: Chen H, McGowan EM, Ren N, Lal S, Nassif N, Shad-Kaneez F, Qu X, Lin Y Tags: Biomark Insights Source Type: research

High Frequency of Extractable Nuclear Autoantibodies in Wheat-Related Disorders.
Conclusions: High proportions of wheat-related disease subjects carry ENA antibodies that are important specific biomarkers of autoimmunity. PMID: 29977112 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - July 8, 2018 Category: General Medicine Authors: Yang Y, Krishna K, Deshpande P, Ranganathan V, Jayaraman V, Wang T, Bei K, Krishnamurthy H Tags: Biomark Insights Source Type: research

Cytokine Gene Polymorphisms in Saudi Patients With Atopic Dermatitis: A Case-Control Study.
Abstract The cause of atopic dermatitis (AD) is multifactorial and a number of genes including cytokines have been involved. We genotyped 315 subjects for polymorphisms in TNF-α and TNF-β and IL-10 genes. Patients had significantly higher frequency of GA genotype of TNF-α (-308 G/A) than healthy controls. Patients with AD and controls had similar distribution of A and G alleles. Genotype AA was found in 7.11% of controls while completely absent in cases. The frequencies of genotypes GG and AA of TNF-β (+252 A/G) polymorphism were higher whereas the frequency of genotype GA was significantly l...
Source: Biomarker Insights - June 12, 2018 Category: General Medicine Authors: Bin Huraib G, Al Harthi F, Arfin M, Al-Sugheyr M, Rizvi S, Al-Asmari A Tags: Biomark Insights Source Type: research

Impaired Cerebral Perfusion in Multiple Sclerosis: Relevance of Endothelial Factors.
Abstract Magnetic resonance imaging techniques measuring in vivo brain perfusion and integrity of the blood-brain barrier have developed rapidly in the past decade, resulting in a wide range of available methods. This review first discusses their principles, possible pitfalls, and potential for quantification and outlines clinical application in neurological disorders. Then, we focus on the endothelial cells of the blood-brain barrier, pointing out their contribution in regulating vascular tone by production of vasoactive substances. Finally, the role of these substances in brain hypoperfusion in multiple sclerosi...
Source: Biomarker Insights - June 1, 2018 Category: General Medicine Authors: Monti L, Morbidelli L, Rossi A Tags: Biomark Insights Source Type: research

The Therapeutic Potential of Blocking Galectin-3 Expression in Acute Myocardial Infarction and Mitigating Inflammation of Infarct Region: A Clinical Outcome-Based Translational Study.
Conclusions: Galectin-3 is directly associated with early myocardial inflammation post-MI and may represent a potential target for therapeutic inhibition. PMID: 29769800 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - May 19, 2018 Category: General Medicine Authors: Mosleh W, Chaudhari MR, Sonkawade S, Mahajan S, Khalil C, Frodey K, Shah T, Dahal S, Karki R, Katkar R, Blankesteijn WM, Page B, Pokharel S, Kim M, Sharma UC Tags: Biomark Insights Source Type: research

Lipidomic Profiling of Plasma and Erythrocytes From Septic Patients Reveals Potential Biomarker Candidates.
Conclusions: These results suggest that lipidome profiling has great potential in discovering potential clinical biomarkers for sepsis and helping to understand its underlying mechanisms. PMID: 29623000 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - April 8, 2018 Category: General Medicine Authors: Mecatti GC, Fernandes Messias MC, Sant'Anna Paiola RM, Figueiredo Angolini CF, da Silva Cunha IB, Eberlin MN, de Oliveira Carvalho P Tags: Biomark Insights Source Type: research

Technical Validation of a Reverse-Transcription Quantitative Polymerase Chain Reaction In Vitro Diagnostic Test for the Determination of MiR-31-3p Expression Levels in Formalin-Fixed Paraffin-Embedded Metastatic Colorectal Cancer Tumor Specimens.
, Gaston-Mathé Y Abstract MiR-31-3p expression has been shown to be a predictive biomarker for response to anti-epithelial growth factor receptor therapy in patients with RAS wild-type metastatic colorectal cancer (mCRC). To aid in the quantification of miR-31-3p expression in formalin-fixed paraffin-embedded (FFPE) primary tumor samples from patients with mCRC, a reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay was developed and validated. Assay development included the identification of a microRNA reference standard and the determination of an appropriate relative quantificatio...
Source: Biomarker Insights - March 27, 2018 Category: General Medicine Authors: Ramon L, David C, Fontaine K, Lallet E, Marcaillou C, Martin-Lannerée S, Decaulne V, Vazart C, Gélibert AH, Abdelali RB, Costa JM, Rousseau F, Thiébaut R, Yost L, Gaston-Mathé Y Tags: Biomark Insights Source Type: research

Reactive Oxygen Species: A Key Constituent in Cancer Survival.
Conclusions: This review focuses the role of ROS in cancer survival pathways and importance of targeting the ROS signal involved in cancer development, which is a new strategy in cancer treatment. PMID: 29449774 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - February 18, 2018 Category: General Medicine Authors: Kumari S, Badana AK, G MM, G S, Malla R Tags: Biomark Insights Source Type: research

Prion Proteins Without the Glycophosphatidylinositol Anchor: Potential Biomarkers in Neurodegenerative Diseases.
bec V Abstract Prion protein (PrP) is a biomolecule that is involved in neuronal signaling, myelinization, and the development of neurodegenerative diseases. In the cell, PrP is shed by the ADAM10 protease. This process generates PrP molecules that lack glycophosphatidylinositol anchor, and these molecules incorporate into toxic aggregates and neutralize toxic oligomers. Due to this dual role, these molecules are important biomarkers for neurodegenerative diseases. In this review, we present shed PrP as a potential biomarker, with a focus on PrP226*, which may be the main biomarker for predicting neurodegenerative...
Source: Biomarker Insights - February 18, 2018 Category: General Medicine Authors: Kovač V, Čurin Šerbec V Tags: Biomark Insights Source Type: research

Nkx2-3-A Slippery Slope From Development Through Inflammation Toward Hematopoietic Malignancies.
;, Balogh P Abstract The development of peripheral lymphoid tissues from the mesoderm is the result of a complex convergence combining lymphohematopoietic differentiation with the local specification of nonhematopoietic mesenchymal components. Although the various transcriptional regulators with fate-determining effects in diversifying the mobile leukocyte subsets have been thoroughly studied and identified, the tissue-specific determinants promoting the regional differentiation of resident mesenchyme are less understood. Of these factors, various members of the NK-class Nkx paralogues have emerged as key regulato...
Source: Biomarker Insights - February 18, 2018 Category: General Medicine Authors: Vojkovics D, Kellermayer Z, Kajtár B, Roncador G, Vincze Á, Balogh P Tags: Biomark Insights Source Type: research

A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W.
Abstract Objective: Using compound W (a 3,3'-diiodothyronine sulfate [T2S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T2S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection ...
Source: Biomarker Insights - February 18, 2018 Category: General Medicine Authors: Huang B, Yu H, Bao J, Zhang M, Green WL, Wu SY Tags: Biomark Insights Source Type: research

Evaluation of a Serum Lung Cancer Biomarker Panel.
Conclusions: This study validates the accuracy of a panel of proteins and an autoantibody in a population relevant to lung cancer detection and suggests a benefit to combining clinical features with the biomarker results. PMID: 29371783 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - January 28, 2018 Category: General Medicine Authors: Mazzone PJ, Wang XF, Han X, Choi H, Seeley M, Scherer R, Doseeva V Tags: Biomark Insights Source Type: research

Kinetics of Procalcitonin in Pediatric Patients on Extracorporeal Membrane Oxygenation.
Conclusions: Procalcitonin does not seem to be modified by ECMO and could be a good biomarker of evolution. PMID: 29343939 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - January 19, 2018 Category: General Medicine Authors: Bobillo S, Rodríguez-Fanjul J, Solé A, Moreno J, Balaguer M, Esteban E, Cambra FJ, Jordan I Tags: Biomark Insights Source Type: research

Plasma Cytochrome c Detection Using a Highly Sensitive Electrochemiluminescence Enzyme-Linked Immunosorbent Assay.
Abstract Background: Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury. Methods and results: We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad dynamic range (2-1200 ng/mL in humans and 5-500 ng/mL in rat) and high assay reproducibility (inter-assay coefficient
Source: Biomarker Insights - December 27, 2017 Category: General Medicine Authors: Radhakrishnan J, Origenes R, Littlejohn G, Nikolich S, Choi E, Smite S, Lamoureux L, Baetiong A, Shah M, Gazmuri RJ Tags: Biomark Insights Source Type: research

Inhaled Steroids and Active Smoking Drive Chronic Obstructive Pulmonary Disease Symptoms and Biomarkers to a Greater Degree Than Airflow Limitation.
Abstract RATIONALE: Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease, and development of novel therapeutics requires an understanding of pathophysiologic phenotypes. OBJECTIVES: The purpose of the Airways Disease Endotyping for Personalized Therapeutics (ADEPT) study was to correlate clinical features and biomarkers with molecular characteristics in a well-profiled COPD cohort. METHODS: A total of 67 COPD subjects (forced expiratory volume in the first second of expiration [FEV1]: 45%-80% predicted) and 63 healthy smoking and nonsmoking controls underwent multiple assessments inclu...
Source: Biomarker Insights - October 1, 2017 Category: General Medicine Authors: Silkoff PE, Singh D, FitzGerald JM, Eich A, Ludwig-Sengpiel A, Chupp GC, Backer V, Porsbjerg C, Girodet PO, Dransfield MT, Baribaud F, Susulic VS, Loza MJ Tags: Biomark Insights Source Type: research

Changes in Alternative Splicing as Pharmacodynamic Markers for Sudemycin D6.
CONCLUSIONS: Exon skipping in the DUSP11 and SRRM1 pre-mRNAs are pharmacodynamic markers for sudemycin D6 treatment and show effects beginning at 9 hours after treatment. PMID: 28932105 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - September 23, 2017 Category: General Medicine Authors: Thurman M, van Doorn J, Danzer B, Webb TR, Stamm S Tags: Biomark Insights Source Type: research

Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1 Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation?
us VB Abstract Monocyte chemoattractant protein-1 (MCP-1) overproduction from inflamed adipose tissue is a major contributor to obesity-related metabolic syndromes. 3T3-L1 embryonic fibroblasts were cultured and differentiated into adipocytes using an established protocol. Adipocytes were treated with lipopolysaccharide (LPS) to induce inflammation and thus MCP-1 release. At the same time, varying concentrations of chondroitin sulfate (CS) were added in a physiologically relevant range (10-200 µg/mL) to determine its impact on MCP-1 release. Chondroitin sulfate, a natural glycosaminoglycan of connecti...
Source: Biomarker Insights - September 13, 2017 Category: General Medicine Authors: Stabler TV, Montell E, Vergés J, Huebner JL, Kraus VB Tags: Biomark Insights Source Type: research

Prognostic Impact of Urokinase Plasminogen Activator Receptor Expression in Pancreatic Cancer: Malignant Versus Stromal Cells.
This study suggests a role for uPAR as a biomarker to single out higher risk subgroups of patients with pancreatic cancer. PMID: 28690396 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - July 13, 2017 Category: General Medicine Authors: de Geus SW, Baart VM, Boonstra MC, Kuppen PJ, Prevoo HA, Mazar AP, Bonsing BA, Morreau H, van de Velde CJ, Vahrmeijer AL, Sier CF Tags: Biomark Insights Source Type: research

Making Meaningful Clinical Use of Biomarkers.
Abstract This review discusses the current state of biomarker discovery for the purposes of diagnostics and therapeutic monitoring. We underscore relevant challenges that have defined the gap between biomarker discovery and meaningful clinical use. We highlight recent advancements in and propose a way to think about future biomarker development. PMID: 28659713 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - July 1, 2017 Category: General Medicine Authors: Selleck MJ, Senthil M, Wall NR Tags: Biomark Insights Source Type: research

Data-Independent Acquisition and Parallel Reaction Monitoring Mass Spectrometry Identification of Serum Biomarkers for Ovarian Cancer.
Abstract A data-independent acquisition (DIA)/parallel reaction monitoring (PRM) workflow was implemented to identify improved ovarian cancer biomarkers. Data-independent acquisition on ovarian cancer versus control sera and literature searches identified 50 biomarkers and indicated that apolipoprotein A-IV (ApoA-IV) is the most significantly differentially regulated protein. Parallel reaction monitoring with Targeted Ovarian Cancer Proteome Assay validated differential ApoA-IV expression and quantified 9 other biomarkers. Random Forest (RF) analyses achieved 92.3% classification accuracy and confirmed ApoA-IV as ...
Source: Biomarker Insights - June 16, 2017 Category: General Medicine Authors: Rauniyar N, Peng G, Lam TT, Zhao H, Mor G, Williams KR Tags: Biomark Insights Source Type: research

Influence of Circulating Endothelin-1 and Asymmetric Dimethylarginine on Whole Brain Circulation Time in Multiple Sclerosis.
Abstract Blood-brain barrier (BBB) breakdown, inflammatory and immune cell activation, and chronic cerebral hypoperfusion are features of multiple sclerosis (MS). The aim is to determine the influence of endothelin-1 (ET1) and asymmetric dimethylarginine (ADMA) on cerebral circulation time (CCT) in patients with MS. In all, 64 patients with MS (39 relapsing-remitting [RR]-MS; 25 secondary progressive [SP]-MS subtype) and 37 controls (C) were studied. Cerebral circulation time was obtained by angiography. Plasmatic ET1 and ADMA were measured by enzyme-linked immunosorbent assay. Lesion load (LL) and brain volume (B...
Source: Biomarker Insights - June 16, 2017 Category: General Medicine Authors: Monti L, Morbidelli L, Bazzani L, Rossi A Tags: Biomark Insights Source Type: research

Soluble P-selectin as a Biomarker for Infection and Survival in Patients With a Systemic Inflammatory Response Syndrome on the Intensive Care Unit.
CONCLUSIONS: This study shows that in patients with SIRS not admitted for infection, sP-selectin levels are significantly related to the subsequent development of infection. Furthermore, patients with higher sP-selectin levels in the first 2 days of admission had higher 30-day mortality, although this association is not statistically significant. Therefore, we conclude that sP-selectin is a potential future biomarker for both mortality and infection in patients with SIRS, but more research is needed to confirm its prognostic role. PMID: 28469394 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - May 6, 2017 Category: Journals (General) Authors: Schrijver IT, Kemperman H, Roest M, Kesecioglu J, de Lange DW Tags: Biomark Insights Source Type: research

Cytochrome P-450 1B1 Leu432Val Polymorphism Does Not Show Association With Breast Cancer in Northern Iranian Women With a History of Infertility.
Abstract The Cytochrome P-4501B1 (CYP1B1) Leu432Val polymorphism has been previously shown to be associated with some types of cancer and affects CYP1B1-mediated metabolism of various infertility drugs. To establish the frequency of CYP1B1 Leu432Val polymorphism among women with a history of infertility drug use, we studied the genotypes of 147 patients with breast cancer with a history of infertility and 150 cancer-free, infertile women (control group) in Northern Iran. A polymerase chain reaction-based restriction fragment length polymorphism assay was used to detect GG (Val/Val), CG (Leu/Val), and CC (Leu/Leu) ...
Source: Biomarker Insights - May 6, 2017 Category: Journals (General) Authors: Andarieh MG, Zabihi E, Moslemi D, Delavar MA, Haji-Ahmadi M, Monfared AS, Jorsaraei SGA, Ghasemi M, Esmaeilzadeh S Tags: Biomark Insights Source Type: research

Expression of Reactive Oxygen Species-Related Transcripts in Egyptian Children With Autism.
Abstract The molecular basis of the pathophysiological role of oxidative stress in autism is understudied. Herein, we used polymerase chain reaction (PCR) array to analyze transcriptional pattern of 84 oxidative stress genes in peripheral blood mononuclear cell pools isolated from 32 autistic patients (16 mild/moderate and 16 severe) and 16 healthy subjects (each sample is a pool from 4 autistic patients or 4 controls). The PCR array data were further validated by quantitative real-time PCR in 80 autistic children (55 mild/moderate and 25 severe) and 60 healthy subjects. Our data revealed downregulation in GCLM, S...
Source: Biomarker Insights - May 6, 2017 Category: Journals (General) Authors: Meguid NA, Ghozlan SAS, Mohamed MF, Ibrahim MK, Dawood RM, Din NGBE, Abdelhafez TH, Hemimi M, Awady MKE Tags: Biomark Insights Source Type: research

Leptomeningeal Carcinomatosis in Gastric Cancer: A Therapeutical Challenge.
We report a case of primary manifestation of LC due to metastatic GC with rapid deterioration and refractory course to conventional and intrathecal chemotherapy. We review the literature and discuss the therapeutic challenges. PMID: 28469397 [PubMed] (Source: Biomarker Insights)
Source: Biomarker Insights - May 6, 2017 Category: Journals (General) Authors: Vergoulidou M Tags: Biomark Insights Source Type: research

An Integrative Genomics Approach for Associating Genome-Wide Association Studies Information With Localized and Metastatic Prostate Cancer Phenotypes.
Abstract High-throughput genotyping has enabled discovery of genetic variants associated with an increased risk of developing prostate cancer using genome-wide association studies (GWAS). The goal of this study was to associate GWAS information of patients with primary organ-confined and metastatic prostate cancer using gene expression data and to identify molecular networks and biological pathways enriched for genetic susceptibility variants involved in the 2 disease states. The analysis revealed gene signatures for the 2 disease states and a gene signature distinguishing the 2 patient groups. In addition, the an...
Source: Biomarker Insights - May 6, 2017 Category: Journals (General) Authors: Hicks C, Ramani R, Sartor O, Bhalla R, Miele L, Dlamini Z, Gumede N Tags: Biomark Insights Source Type: research