Titanium Dioxide Nanoparticles Induce Cell Cycle Arrest and  Apoptosis through Inhibiting PI3K/AKT/mTOR Pathway in Spermatogonia
Biol Trace Elem Res. 2023 Dec 11. doi: 10.1007/s12011-023-03984-7. Online ahead of print.ABSTRACTTitanium dioxide nanoparticles (TiO2 NPs) can result in the reduction of sperm numbers, but the mechanisms have not been well elucidated. The purpose of this study was to investigate the effects of TiO2 NPs on cell cycle and apoptosis in spermatogonia and to explore the role of PI3K/AKT/mTOR signaling pathway in this process. The mouse spermatogonia cell line (GC-1) was treated with TiO2 NPs at different concentrations (0, 25, 50, 75 and 100 μg/mL) for 24 h to detect cell viability, cell cycle, apoptosis, and key proteins rela...
Source: Biological Trace Element Research - December 11, 2023 Category: Biology Authors: Ruoyun Dong Xiaojia Meng Hongmei Chang Yuzhu Lei Yunhua Hu Yizhong Yan Guanling Song Source Type: research

Does the Presence of Heavy Metals Influence the Gene Expression and Oxidative Stress in Bladder Cancer?
Biol Trace Elem Res. 2023 Dec 11. doi: 10.1007/s12011-023-03950-3. Online ahead of print.ABSTRACTHeavy metal toxicity is associated with cancer progression. Studies have reported the relation between some metal ions and bladder cancer (BC). Direct influence of such agents in bladder carcinogenesis is still needed. Total 49 BC patients were included in the study. Level of Pb, Cr, Hg and Cd, oxidative stress markers, and gene expression of Bcl-2, Bax, IL-6, AKT, and P38 genes were detected in cancer and non-cancerous tissues obtained from bladder cancer patients. Concentrations of Pb, Cr, and Cd were significantly elevated i...
Source: Biological Trace Element Research - December 10, 2023 Category: Biology Authors: Eslam El-Agrody Hassan Abol-Enein Wael I Mortada Amira Awadalla Heba H Tarabay Om-Ali Elkhawaga Source Type: research

Does the Presence of Heavy Metals Influence the Gene Expression and Oxidative Stress in Bladder Cancer?
Biol Trace Elem Res. 2023 Dec 11. doi: 10.1007/s12011-023-03950-3. Online ahead of print.ABSTRACTHeavy metal toxicity is associated with cancer progression. Studies have reported the relation between some metal ions and bladder cancer (BC). Direct influence of such agents in bladder carcinogenesis is still needed. Total 49 BC patients were included in the study. Level of Pb, Cr, Hg and Cd, oxidative stress markers, and gene expression of Bcl-2, Bax, IL-6, AKT, and P38 genes were detected in cancer and non-cancerous tissues obtained from bladder cancer patients. Concentrations of Pb, Cr, and Cd were significantly elevated i...
Source: Biological Trace Element Research - December 10, 2023 Category: Biology Authors: Eslam El-Agrody Hassan Abol-Enein Wael I Mortada Amira Awadalla Heba H Tarabay Om-Ali Elkhawaga Source Type: research

Low-Dose Hexavalent Chromium Exposure Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Rat Liver
This study investigated the toxic effects of low-dose hexavalent chromium (Cr(VI)) on rat liver. Male specific pathogen-free (SPF) Sprague-Dawley (SD) rats (4-5 weeks of age) were randomly divided into groups: saline, 0.05 mg/kg Cr(VI), and 0.25 mg/kg Cr(VI). The rats were subjected to intratracheal instillation of K2Cr2O7 suspensions or saline once weekly, for a total of five times. The results showed that the accumulation of Cr(VI) in the blood of the 0.25 mg/kg K2Cr2O7 group was significantly higher than that in the saline group. Transmission electron microscopy (TEM) showed that exposure to hexavalent chromium caused e...
Source: Biological Trace Element Research - December 8, 2023 Category: Biology Authors: Xiaoying Li Ningning Li Xiuzhi Zhang Lixia Zhang Guang Jia Shanfa Yu Source Type: research

Microchemistry of Single Hair Strands Below and Above the Scalp: Impacts of External Contamination on Cuticle and Cortex Layers
Biol Trace Elem Res. 2023 Dec 8. doi: 10.1007/s12011-023-03973-w. Online ahead of print.ABSTRACTExternal contamination of hair is the most significant challenge to it becoming an accepted matrix for monitoring endogenous metal exposure and nutritional deficiency. Here we use laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to quantify elemental concentrations in hair strands below and above the scalp in the cuticle and cortex layers to determine the extent of external contamination in a reference population. Evidence of hair strand contamination occurred for barium, calcium, iron, magnesium, and stro...
Source: Biological Trace Element Research - December 8, 2023 Category: Biology Authors: Jennie R Christensen Geriene O LaBine Source Type: research

Effects of Zinc-Gluconate Levels on Growth Performance, Whole-Body Composition, and Mineral and Enzyme Activities of Juvenile Mori (Cirrhinus mrigala)
Biol Trace Elem Res. 2023 Dec 8. doi: 10.1007/s12011-023-03983-8. Online ahead of print.ABSTRACTZinc (Zn) is an important dietary nutrient for the optimum growth performance, feed efficiency, normal metabolism, and fish health. Unfortunately, Zn requirement for mori (Cirrhinus mrigala) is not available. This experiment was designed to assess the impacts of Zn-gluconate levels on growth performance, feed utilization, mineral composition, and enzyme activities of mori and determines the optimum requirement of Zn-gluconate for this species. For this purpose, seven isonitrogenous (29.18%) and isolipidic (10.71%) purified diets...
Source: Biological Trace Element Research - December 8, 2023 Category: Biology Authors: Zainab Hamid Syed Zakir Hussain Shah Mahroze Fatima None Maryam Hafsa Nadeem Wazir Ali Source Type: research

Low-Dose Hexavalent Chromium Exposure Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Rat Liver
This study investigated the toxic effects of low-dose hexavalent chromium (Cr(VI)) on rat liver. Male specific pathogen-free (SPF) Sprague-Dawley (SD) rats (4-5 weeks of age) were randomly divided into groups: saline, 0.05 mg/kg Cr(VI), and 0.25 mg/kg Cr(VI). The rats were subjected to intratracheal instillation of K2Cr2O7 suspensions or saline once weekly, for a total of five times. The results showed that the accumulation of Cr(VI) in the blood of the 0.25 mg/kg K2Cr2O7 group was significantly higher than that in the saline group. Transmission electron microscopy (TEM) showed that exposure to hexavalent chromium caused e...
Source: Biological Trace Element Research - December 8, 2023 Category: Biology Authors: Xiaoying Li Ningning Li Xiuzhi Zhang Lixia Zhang Guang Jia Shanfa Yu Source Type: research

Microchemistry of Single Hair Strands Below and Above the Scalp: Impacts of External Contamination on Cuticle and Cortex Layers
Biol Trace Elem Res. 2023 Dec 8. doi: 10.1007/s12011-023-03973-w. Online ahead of print.ABSTRACTExternal contamination of hair is the most significant challenge to it becoming an accepted matrix for monitoring endogenous metal exposure and nutritional deficiency. Here we use laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to quantify elemental concentrations in hair strands below and above the scalp in the cuticle and cortex layers to determine the extent of external contamination in a reference population. Evidence of hair strand contamination occurred for barium, calcium, iron, magnesium, and stro...
Source: Biological Trace Element Research - December 8, 2023 Category: Biology Authors: Jennie R Christensen Geriene O LaBine Source Type: research

Effects of Zinc-Gluconate Levels on Growth Performance, Whole-Body Composition, and Mineral and Enzyme Activities of Juvenile Mori (Cirrhinus mrigala)
Biol Trace Elem Res. 2023 Dec 8. doi: 10.1007/s12011-023-03983-8. Online ahead of print.ABSTRACTZinc (Zn) is an important dietary nutrient for the optimum growth performance, feed efficiency, normal metabolism, and fish health. Unfortunately, Zn requirement for mori (Cirrhinus mrigala) is not available. This experiment was designed to assess the impacts of Zn-gluconate levels on growth performance, feed utilization, mineral composition, and enzyme activities of mori and determines the optimum requirement of Zn-gluconate for this species. For this purpose, seven isonitrogenous (29.18%) and isolipidic (10.71%) purified diets...
Source: Biological Trace Element Research - December 8, 2023 Category: Biology Authors: Zainab Hamid Syed Zakir Hussain Shah Mahroze Fatima None Maryam Hafsa Nadeem Wazir Ali Source Type: research

Regulating Enzyme Catalysis by Tailored Silver Nanocrystals Fabricated with Holigarna arnottiana-Synthesis, Characterization, and Performance Optimization
Biol Trace Elem Res. 2023 Dec 7. doi: 10.1007/s12011-023-03981-w. Online ahead of print.ABSTRACTModification of catalytic expression of enzymes and regulating their in vivo activity are the goals of novel treatment strategies. A green synthetic nanostructured silver with potent trypsin inhibitory properties has not yet been developed, despite the fact that silver nanoparticles possess unique properties that allow them to efficiently block enzymes. The present study demonstrates for the first time a facile, safe, economic, and eco-friendly synthetic route for silver nanoparticles using an aqueous extract of Holigarna arnott...
Source: Biological Trace Element Research - December 7, 2023 Category: Biology Authors: Neethu George D Gayathri Devi Source Type: research

Chronic Iodine Intake Excess Damages the Structure of Articular Cartilage and Epiphyseal Growth Plate
This study aimed to explore the influence of excess iodine on the articular cartilage and epiphyseal growth plate in rats. Wistar rats (n = 200) were randomly divided into five groups with 40 rats in each: normal iodine (NI), 5-fold high iodine group (5HI), 10-fold high iodine group (10HI), 50-fold high iodine group (50HI), and 100-fold high iodine group (100HI). The rats were executed in 6 and 12 months. 24-h urinary iodine concentration (UIC) was monitored by arsenic-cerium catalytic spectrophotometry. The chemiluminescence method was used to determine the thyroid function. The pathological changes in the epiphyseal plat...
Source: Biological Trace Element Research - December 7, 2023 Category: Biology Authors: Ying Zhang Xin Zhao Le Shan Miao Liu Zixuan Zhang Zeji Wang Xinbao Zhang Haohao Meng Yan Song Wanqi Zhang Zhongna Sang Source Type: research

Regulating Enzyme Catalysis by Tailored Silver Nanocrystals Fabricated with Holigarna arnottiana-Synthesis, Characterization, and Performance Optimization
Biol Trace Elem Res. 2023 Dec 7. doi: 10.1007/s12011-023-03981-w. Online ahead of print.ABSTRACTModification of catalytic expression of enzymes and regulating their in vivo activity are the goals of novel treatment strategies. A green synthetic nanostructured silver with potent trypsin inhibitory properties has not yet been developed, despite the fact that silver nanoparticles possess unique properties that allow them to efficiently block enzymes. The present study demonstrates for the first time a facile, safe, economic, and eco-friendly synthetic route for silver nanoparticles using an aqueous extract of Holigarna arnott...
Source: Biological Trace Element Research - December 7, 2023 Category: Biology Authors: Neethu George D Gayathri Devi Source Type: research

Chronic Iodine Intake Excess Damages the Structure of Articular Cartilage and Epiphyseal Growth Plate
This study aimed to explore the influence of excess iodine on the articular cartilage and epiphyseal growth plate in rats. Wistar rats (n = 200) were randomly divided into five groups with 40 rats in each: normal iodine (NI), 5-fold high iodine group (5HI), 10-fold high iodine group (10HI), 50-fold high iodine group (50HI), and 100-fold high iodine group (100HI). The rats were executed in 6 and 12 months. 24-h urinary iodine concentration (UIC) was monitored by arsenic-cerium catalytic spectrophotometry. The chemiluminescence method was used to determine the thyroid function. The pathological changes in the epiphyseal plat...
Source: Biological Trace Element Research - December 7, 2023 Category: Biology Authors: Ying Zhang Xin Zhao Le Shan Miao Liu Zixuan Zhang Zeji Wang Xinbao Zhang Haohao Meng Yan Song Wanqi Zhang Zhongna Sang Source Type: research

Effects of Zinc Compounds on Lysozyme, Peroxidase, and α-Amylase from the Perspective of Oral Health: a Scoping Review
In conclusion, for zinc to be used as an effective topical therapeutic agent for oral health, further studies on the activity of human salivary enzymes are warranted, and additional information regarding the type and concentration of effective zinc compounds is also required.PMID:38057484 | DOI:10.1007/s12011-023-03972-x (Source: Biological Trace Element Research)
Source: Biological Trace Element Research - December 6, 2023 Category: Biology Authors: Moon-Jong Kim Jeong-Hyun Kang Hong-Seop Kho Source Type: research

Strontium Attenuates LPS-Induced Inflammation via TLR4/MyD88/NF- κB Pathway in Bovine Ruminal Epithelial Cells
Biol Trace Elem Res. 2023 Dec 7. doi: 10.1007/s12011-023-03992-7. Online ahead of print.ABSTRACTSubacute ruminal acidosis (SARA) is a common nutritional metabolic disease in ruminants that causes significant economic losses to dairy farming. Strontium (Sr) is known to be involved in bone metabolism and exhibits potent anti-inflammatory effects. To evaluate the effect of Sr on inflammation in bovine ruminal epithelial cells, a model of LPS-induced inflammation was established in this study, and the cell viability of bovine ruminal epithelial cells was measured using CCK-8. The production of pro-inflammatory cytokines was me...
Source: Biological Trace Element Research - December 6, 2023 Category: Biology Authors: Panpan Tan Jiaqi Yang Fanxuan Yi Linshan Mei Yazhou Wang Chenxu Zhao Baoyu Zhao Jianguo Wang Source Type: research