Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat
We report age-related decline in expression and cellular localization of Long intergenic noncoding repeat-rich sense-antisense (LINC-RSAS) RNA in the testes and its major cell-types such as primary spermatocytes, Leydig cells and Sertoli cells during ageing of the rat. LINC-RSAS expression in testes increased from immature (4-weeks) to adult (16- and 44-weeks) and declined from adult (44-weeks) to nearly-old (70-weeks) rats. Genomic DNA methylation in the testes showed a similar pattern. Cell-type specific higher expression of LINC-RSAS was observed in primary spermatocytes (pachytene cells), Leydig cells and Sertoli cells...
Source: Biogerontology - February 14, 2024 Category: Geriatrics Authors: Ajay Kumar Danga Sukhleen Kour Anita Kumari Pramod C Rath Source Type: research

Renal aging and mitochondrial quality control
Biogerontology. 2024 Feb 13. doi: 10.1007/s10522-023-10091-6. Online ahead of print.ABSTRACTMitochondria are dynamic organelles that participate in different cellular process that control metabolism, cell division, and survival, and the kidney is one of the most metabolically active organs that contains abundant mitochondria. Perturbations in mitochondrial homeostasis in the kidney can accelerate kidney aging, and maintaining mitochondrial homeostasis can effectively delay aging in the kidney. Kidney aging is a degenerative process linked to detrimental processes. The significance of aberrant mitochondrial homeostasis in r...
Source: Biogerontology - February 13, 2024 Category: Geriatrics Authors: Xiuli Guo Jiao Wang Yinjie Wu Xinwang Zhu Li Xu Source Type: research

A CRISPR base editing approach for the functional assessment of telomere biology disorder-related genes in human health and aging
Biogerontology. 2024 Feb 4. doi: 10.1007/s10522-024-10094-x. Online ahead of print.ABSTRACTTelomere Biology Disorders (TBDs) are a group of rare diseases characterized by the presence of short and/or dysfunctional telomeres. They comprise a group of bone marrow failure syndromes, idiopathic pulmonary fibrosis, and liver disease, among other diseases. Genetic alterations (variants) in the genes responsible for telomere homeostasis have been linked to TBDs. Despite the number of variants already identified as pathogenic, an even more significant number must be better understood. The study of TBDs is challenging since identif...
Source: Biogerontology - February 4, 2024 Category: Geriatrics Authors: Gustavo Borges Yahya Benslimane Lea Harrington Source Type: research

Linking telomere dynamics to evolution, life history and environmental change: perspectives, predictions and problems
Biogerontology. 2024 Jan 22. doi: 10.1007/s10522-023-10081-8. Online ahead of print.ABSTRACTThis perspectives paper considers the value of studying telomere biology outside of a biomedical context. I provide illustrative examples of the kinds of questions that evolutionary ecologists have addressed in studies of telomere dynamics in non-model species, primarily metazoan animals, and what this can contribute to our understanding of their evolution, life histories and health. I also discuss why the predicted relationships between telomere dynamics and life history traits, based on the detailed cellular studies in humans and ...
Source: Biogerontology - January 22, 2024 Category: Geriatrics Authors: Pat Monaghan Source Type: research

Sustained exposure to high glucose induces differential expression of cellular senescence markers in murine macrophages but impairs immunosurveillance response to senescent cells secretome
This study evaluated the impact of chronic hyperglycemia on the induction of cellular senescence and subsequent immunosurveillance functions in RAW264.7 macrophages. Macrophages were cultured under normal glucose (NG; 5 mM), high glucose (HG; 20 mM), and very high glucose (VHG; 40 mM) conditions and assessed for markers of cellular senescence. Hyperglycemia induced strong upregulation of SA-β-gal activity, and loss of PCNA and Lamin B1 gene expression while markers of cell cycle arrest generally decreased. Non-significant changes in SASP-related proteins were observed while ROS levels slightly decreased and mitochondrial ...
Source: Biogerontology - January 19, 2024 Category: Geriatrics Authors: Bhawna Diwan Rahul Yadav Rohit Goyal Rohit Sharma Source Type: research

Seven knowledge gaps in modern biogerontology
Biogerontology. 2024 Feb;25(1):1-8. doi: 10.1007/s10522-023-10089-0.ABSTRACTAbout a year ago, members of the editorial board of Biogerontology were requested to respond to a query by the editor-in-chief of the journal as to what one question within their field of ageing research still needs to be asked and answered. This editorial is inspired by the wide range and variety of questions, ideas, comments and suggestions received in response to that query. The seven knowledge gaps identified in this article are arranged into three main categories: evolutionary aspects of longevity, biological survival and death aspects, and he...
Source: Biogerontology - January 11, 2024 Category: Geriatrics Authors: Suresh I S Rattan Source Type: research

Age and dietary restriction modulate mitochondrial quality in quadriceps femoris muscle of male mice
This study aimed to explore the effect of age and DR on the homeostasis of mitochondrial quality control in skeletal muscle. To study the effect of age on mitochondrial homeostasis, young (3 months old) male C57BL/6J mice were fed ad libitum (AL) until 7 (Young), 14 (Middle), and 19 months (Aged) of age. For the DR intervention, 60% of AL intake was given to the mice at 3 months of age until they reached 19 months of age (16 months). The quadriceps femoris muscle was collected for further analysis. Significant changes in the skeletal muscle were noticed during the transition between middle age and the elderly stages. An ac...
Source: Biogerontology - January 6, 2024 Category: Geriatrics Authors: Ting-Rui Zhang Chun-Hsien Chiang Tzu-Chieh Hsu Chih-Yun Wang Ching-Yi Chen Source Type: research