Biochimica et Biophysica Acta (BBA) Proteins and Proteomics This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Immobilization of lipase from Pseudomonas fluorescens on glyoxyl-octyl-agarose beads: Improved stability and reusability
Publication date: Available online 12 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Nathalia S. Rios, Carmen Mendez-Sanchez, Sara Arana-Peña, Nazzoly Rueda, Claudia Ortiz, Luciana R.B. Gonçalves, Roberto Fernandez-LafuenteAbstractThe lipase from Pseudomonas fluorescens (PFL) has been immobilized on glyoxyl-octyl agarose and compared to the enzyme immobilized on octyl-agarose. Thus, PFL was immobilized at pH 7 on glyoxyl-octyl support via lipase interfacial activation and later incubated at pH 10.5 for 20 h before reduction to get some enzyme-support covalent bonds. This pe...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 13, 2019 Category: Biochemistry Source Type: research

Pitfalls and opportunities in the characterization of unconventionally secreted proteins by secretome analysis
Publication date: Available online 12 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Jessica Schira-Heinen, Leonie Grube, Daniel M. Waldera-Lupa, Falk Baberg, Maike Langini, Omid Etemad-Parishanzadeh, Gereon Poschmann, Kai StühlerAbstractProteins are released from cells by different secretory pathways. The secretory pathway via the ER-Golgi route - realized by a signal sequence - is referred to as “classical secretion”. In contrast, alternative secretory pathways were summarized as “unconventional protein secretion”. Until now, unconventional protein secretion was lacking ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 13, 2019 Category: Biochemistry Source Type: research

Structural features of cold-adapted dimeric GH2 β-D-galactosidase from Arthrobacter sp. 32cB
Publication date: Available online 10 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Maria Rutkiewicz, Anna Bujacz, Grzegorz BujaczAbstractCrystal structures of cold-adapted β-D-galactosidase (EC 3.2.1.23) from the Antarctic bacterium Arthrobacter sp. 32cB (ArthβDG) have been determined in an unliganded form resulting from diffraction experiments conducted at 100 K (at resolution 1.8 Å) and at room temperature (at resolution 3.0 Å). A detailed comparison of those two structures of the same enzyme was performed in order to estimate differences in their molecular flexibilit...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 12, 2019 Category: Biochemistry Source Type: research

Bacterial functional amyloids: Order from disorder
Publication date: Available online 10 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Neha Jain, Matthew R. ChapmanAbstractThe discovery of intrinsic disorderness in proteins and peptide regions has given a new and useful insight into the working of biological systems. Due to enormous plasticity and heterogeneity, intrinsically disordered proteins or regions in proteins can perform myriad of functions. The flexibility in disordered proteins allows them to undergo conformation transition to form homopolymers of proteins called amyloids. Amyloids are highly structured protein aggregat...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 12, 2019 Category: Biochemistry Source Type: research

The cytoplasmic portion of the T3SS inner membrane ring components sort into distinct families based on biophysical properties
Publication date: Available online 10 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Meenakumari Muthuramalingam, C. Russell Middaugh, William D. PickingAbstractType III secretion systems are used by many Gram-negative bacteria to inject effector proteins into eukaryotic cells to subvert their normal activities. Structurally conserved portions of the type III secretion apparatus include a: basal body located within the bacterial envelope; an exposed needle with tip complex that delivers effectors across the target cell membrane; and cytoplasmic sorting platform that selects cargo a...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 12, 2019 Category: Biochemistry Source Type: research