Biochimica et Biophysica Acta (BBA) Proteins and Proteomics Biochimica et Biophysica Acta (BBA) Proteins and Proteomics RSS feedThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Monomeric and homotrimeric solution structures of truncated human peroxidasin 1 variants
Publication date: Available online 8 July 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Martina Paumann-Page, Rupert Tscheliessnig, Benjamin Sevcnikar, Romy-Sophie Katz, Irene Schwartz, Stefan Hofbauer, Vera Pfanzagl, Paul G. Furtmüller, Christian ObingerAbstractHuman peroxidasin 1 is a multidomain peroxidase situated in the basement membrane. The iron enzyme with covalently bound heme oxidizes bromide to hypobromous acid which facilitates the formation of distinct sulfilimine cross-links in the collagen IV network and therefore contributes to its mechanical stability. Additional to t...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - July 10, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: September 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1867, Issue 9Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - July 10, 2019 Category: Biochemistry Source Type: research

Constant domain-exchanged Fab enables specific light chain pairing in heterodimeric bispecific SEED-antibodies
ConclusionIncorporation of a constant domain-exchanged Fab fragment into a SEED antibody yields functional molecules with favorable biophysical properties.General significanceOur results show that the novel engineered bispecific SEED antibody scaffold with an incorporated Fab fragment with CH3-exchanged constant domains is a promising tool for the generation of complete heterodimeric bispecific antibodies with correct light chain pairing.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - July 9, 2019 Category: Biochemistry Source Type: research

Glycosylation effects on the structure and dynamics of a full-length Cel7A cellulase
We report here evidence that targeting Cel7A linker flexibility by point mutations including modification of glycosylation sites could be a promising design strategy to improve cellulase activity.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - July 5, 2019 Category: Biochemistry Source Type: research

Foreword
Publication date: Available online 2 July 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - July 3, 2019 Category: Biochemistry Source Type: research

A comprehensive comparison of the metazoan tryptophan degrading enzymes
Publication date: Available online 2 July 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Hajime Julie YuasaAbstractTryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) have an independent origin; however, they have distinctly evolved to catalyze the same reaction. In general, TDO is a single-copy gene in each metazoan species, and TDO enzymes demonstrate similar enzyme activity regardless of their biological origin. In contrast, multiple IDO paralogues are observed in many species, and they display various enzymatic properties. Similar to vertebrate IDO2, invertebrate ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - July 3, 2019 Category: Biochemistry Source Type: research

Editorial on intrinsically disordered proteins: Amyloid formation and phase separation
Publication date: Available online 25 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Samrat Mukhopadhyay (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 30, 2019 Category: Biochemistry Source Type: research

Inhibition of Plasmodium falciparum cysteine protease falcipain-2 by a human cross-class inhibitor serpinB3: A mechanistic insight
This study explored the possibility of inhibition of FP2 by SPB3. It turned out that general proteolytic activities as well as specific hemoglobinolytic activity of FP2 have been inhibited by SPB3. Furthermore, studies have been designed to investigate and characterize the mechanism of inhibition in comparison with proteases Cathepsin L (CTSL) and papain. The Ki value of inhibition for FP2, measured against its specific substrate (VLK-pNA), is 338.11 nM and stoichiometry (I/E ratio) of inhibition is 1. These values are comparable to CTSL and papain. Analytical gel filtration profile and CD spectroscopy data confirm FP2-S...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 25, 2019 Category: Biochemistry Source Type: research

Guest Editorial
Publication date: Available online 25 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 25, 2019 Category: Biochemistry Source Type: research

Characterization of the 20S proteasome of the lepidopteran, Spodoptera frugiperda
In this report, several forms of 20S proteasomes from extracts of Spodoptera frugiperda (Sf9) cells were separated using electrophoresis in a native polyacrylamide gel and examined for proteolytic activity in the gel and by Western blotting. Distinct proteasome bands isolated from the gel were subjected to liquid chromatography-tandem mass spectrometry and identified as free core particles (CP) and complexes of CP with one or two dimers of assembly chaperones PAC1-PAC2 and activators PA28γ or PA200. In contrast to the activators PA28γ and PA200 that regulate the access of protein substrates to the internal proteolytic ch...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 20, 2019 Category: Biochemistry Source Type: research

Assembly of α-synuclein aggregates on phospholipid bilayers
Publication date: Available online 19 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Zhengjian Lv, Mohtadin Hashemi, Siddhartha Banerjee, Karen Zagorski, Jean-Christophe Rochet, Yuri L. LyubchenkoAbstractThe spontaneous self-assembly of α-synuclein (α-syn) into aggregates of different morphologies is associated with the development of Parkinson's disease. However, the mechanism behind the spontaneous assembly remains elusive. The current study shows a novel effect of phospholipid bilayers on the assembly of the α-syn aggregates. Using time-lapse atomic force microscopy, it was d...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 19, 2019 Category: Biochemistry Source Type: research

Structure-based reconstruction of a Mycobacterium hypothetical protein into an active Δ5–3-ketosteroid isomerase
Publication date: Available online 19 June 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Fei Peng, Xiyao Cheng, Hongwei Wang, Shikui Song, Tian Chen, Xin Li, Yijun He, Yongqi Huang, Sen Liu, Fei Yang, Zhengding SuAbstractProtein engineering based on structure homology holds the potential to engineer steroid-transforming enzymes on demand. Based on the genome sequencing analysis of industrial Mycobacterium strain HGMS2 to produce 4-androstene-3,17-dione (4-AD), three hypothetical proteins were predicted as putative Δ5–3-ketosteroid isomerases (KSIs) to catalyze an intramolecular prot...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 19, 2019 Category: Biochemistry Source Type: research

Structural characterization of the N-terminal kinase-interacting domain of an Hsp90-cochaperone Cdc37 by CD and solution NMR spectroscopy
We examined the structural and dynamic properties of several fragment proteins corresponding to the N-terminal region of Cdc37 by circular dichroism and solution NMR spectroscopy. We found that the N-terminal domain of Cdc37 exhibits highly dynamic structure, and it exists in the equilibrium between α-helical and more disordered structures. We also found that phosphorylation at Ser13 did not significantly change the overall structure of N-terminal fragment protein of Cdc37. The results suggested that more complicated mechanisms might be necessary to explain the phosphorylation-activated interaction of Cdc37 with various k...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 19, 2019 Category: Biochemistry Source Type: research

Cleavage C-terminal to Asp leads to covalent crosslinking of long-lived human proteins
In this study, proteomics was used to characterize sites of covalent protein-protein crosslinking and identify a novel mechanism of protein-protein crosslinking in the adult human lens. In this mechanism, Lys residues are crosslinked to C-terminal Asp residues that are formed by non-enzymatic protein truncation. Ten different crosslinks were identified in lens major proteins such as αA-crystallin, αB-crystallin and AQP0. Crosslinking in AQP0 increased significantly with age and also increased significantly in cataract lenses compared with normal lenses. Using model peptides, a mechanism of formation of the Lys-Asp crossl...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 19, 2019 Category: Biochemistry Source Type: research

SIRT 1 binding with PKM and NSE and modulate their acetylation and activities
In this study, we aim to identify those novel interacting partners of SIRT1 in rat brain tissue. By using a pre-clear GST-Pull down assay followed by the LC-MS/MS analysis, we've identified potential SIRT1's interacting partners, which function annotation by GO and KEGG analysis indicating some metabolic pathways are among the most enriched. Then we confirmed two candidates Enolase-1 (and NSE (Neuron-Specific Enolase) in brain) and PKM (Pyruvate Kinase Muscle) are associated with SIRT1 in brain tissue lysis by co-immunoprecipitation. Furthermore, increase or decrease the SIRT1 enzyme activity by its agonist SRT1720 or anta...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - June 15, 2019 Category: Biochemistry Source Type: research