Interaction between immunoglobulin G and peroxidase-like iron oxide nanoparticles: Physicochemical and structural features of the protein
Publication date: Available online 29 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Anna V. Bychkova, Mariia V. Lopukhova, Luybov A. Wasserman, Pavel G. Pronkin, Yevgeniy N. Degtyarev, Alexander I. Shalupov, Alexandra D. Vasilyeva, Lyubov’ V. Yurina, Alexander L. Kovarski, Alexey S. Kononikhin, Evgene N. NikolaevAbstractThe study is devoted to the oxidative modification of immunoglobulin G (IgG) on the surface of peroxidase-like iron oxide magnetic nanoparticles (MNPs) under conditions of induced reactive oxygen species (ROS) generation and without them. A pronounced change o...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 29, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: December 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1867, Issue 12Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 26, 2019 Category: Biochemistry Source Type: research

Effects of lithospermic acid on hIAPP aggregation and amyloid-induced cytotoxicity by multiple analytical methods
In this study, electrospray ionization coupled with ion mobility–mass spectrometry, thioflavin T fluorescence assay, Congo red binding assay, Nile red fluorescence assay, circular dichroism spectroscopy, transmission electron microscopy, cell toxicity, lactate dehydrogenase assay (LDH) assay and molecular docking were combined to explore the influence of LA on hIAPP aggregation. Results showed that LA had favorable binding affinity to hIAPP and formed hIAPP–LA complexes, which could alter the relative abundance of the compact and extended conformers and promoted the transition of extended structures to compact conforme...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 15, 2019 Category: Biochemistry Source Type: research

The impact of physiological stress conditions on protein structure and trypsin inhibition of serine protease inhibitor Kazal type 1 (SPINK1) and its N34S variant
Publication date: Available online 13 September 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Ina Buchholz, Felix Nagel, Annelie Klein, Preshit R. Wagh, Ujjwal M. Mahajan, Andreas Greinacher, Markus M. Lerch, Julia Mayerle, Mihaela DelceaAbstractOne of the most common mutations in the serine protease inhibitor Kazal type 1 (SPINK1) gene is the N34S variant which is strongly associated with chronic pancreatitis. Although it is assumed that N34S mutation constitutes a high-risk factor, the underlying pathologic mechanism is still unknown. In the present study, we investigated the impact ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 14, 2019 Category: Biochemistry Source Type: research

Structural studies of the Hsp70/Hsp90 organizing protein of Plasmodium falciparum and its modulation of Hsp70 and Hsp90 ATPase activities
Publication date: Available online 13 September 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Noeli S.M. Silva, Dayane E. Bertolino-Reis, Paulo R. Dores-Silva, Fátima B. Anneta, Thiago V. Seraphim, Leandro R.S. Barbosa, Júlio C. BorgesAbstractHOP is a cochaperone belonging to the foldosome, a system formed by the cytoplasmic Hsp70 and Hsp90 chaperones. HOP acts as an adapter protein capable of transferring client proteins from the first to the second molecular chaperone. HOP is a modular protein that regulates the ATPase activity of Hsp70 and Hsp90 to perform its function. To obtain ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 14, 2019 Category: Biochemistry Source Type: research

Editorial Board
Publication date: November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1867, Issue 11Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 12, 2019 Category: Biochemistry Source Type: research

Publisher's note
Publication date: November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1867, Issue 11Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 12, 2019 Category: Biochemistry Source Type: research

A new sensitive spectrofluorimetric method for measurement of activity and kinetic study of cholinesterases
Publication date: Available online 10 September 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Aliya R. Mukhametgalieva, Irina V. Zueva, Aliya R. Aglyamova, Sofya V. Lushchekina, Patrick MassonAbstractA new spectrofluorimetric method more sensitive than the Ellman method was developed for determination of both acetylcholinesterase and butyrylcholinesterase activity and for kinetic analysis of these enzymes and their mutants. Two selected mutants of human butyrylcholinesterase (E197Q and E197G) were included in this work. As for the Ellman's method, substrates are thiocholine esters, but...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 11, 2019 Category: Biochemistry Source Type: research

Unconventional protein secretion: The hidden pathways
Publication date: Available online 6 September 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Kai Stühler, Kerstin Schipper, Gereon Poschmann (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 7, 2019 Category: Biochemistry Source Type: research

Conventional and non-conventional applications of β-galactosidases
This article reviews the main applications of β-galactosidase, going from its conventional use related to its hydrolytic activity to the ongoing non-conventional applications in the synthesis of high added-value oligosaccharides based on its transgalactosylation activity.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 6, 2019 Category: Biochemistry Source Type: research

Expression and role of CYP505A1 in pathogenicity of Fusarium oxysporum f. sp. lactucae
ConclusionsThe ability of CYP505A1 to oxidize fatty acids present in the cortical cell membranes together with its differential expression in its Fusarium antagonistic form point out to the possibility that this enzyme is associated with Fusarium pathogenicity in lettuce.General significanceThe CYP505 clan is present in pathogenic fungal phyla, making CYP505A1 enzyme a putative candidate as a new target for the development of novel antifungal molecules.Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 4, 2019 Category: Biochemistry Source Type: research

Potential complementation effects of two disease-associated mutations in tetrameric glutaryl-CoA dehydrogenase is due to inter subunit stability-activity counterbalance
Publication date: Available online 3 September 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Joana V. Ribeiro, Tânia G. Lucas, Peter Bross, Cláudio M. Gomes, Bárbara J. HenriquesAbstractGlutaric Aciduria Type I (GA-I), is an autosomal recessive neurometabolic disease caused by mutations in the GCDH gene that encodes for glutaryl-CoA dehydrogenase (GCDH), a flavoprotein involved in the metabolism of tryptophan, lysine and hydroxylysine. Although over 200 disease mutations have been reported a clear correlation between genotype and phenotype has been difficult to establish. To contrib...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - September 4, 2019 Category: Biochemistry Source Type: research

Chaperone potential of erythroid spectrin: Effects of hemoglobin interaction, macromolecular crowders, phosphorylation and glycation
Publication date: Available online 27 August 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Dipayan Bose, Abhijit ChakrabartiAbstractSpectrin, the major protein component of the erythrocyte membrane skeleton has chaperone like activity and is known to bind membrane phospholipids and hemoglobin. We have probed the chaperone activity of spectrin in presence of hemoglobin and phospholipid SUVs of different compositions to elucidate the effect of phospholipid/hemoglobin binding on chaperone function. It is seen that spectrin displays a preference for hemoglobin over other substrates leading...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - August 29, 2019 Category: Biochemistry Source Type: research

Mapping of the binding site for FcμR in human IgM-Fc
Publication date: Available online 23 August 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Rosemary A. Nyamboya, Brian J. Sutton, Rosaleen A. CalvertAbstractFcμR is a high-affinity receptor for the Fc portion of human IgM. It participates in B cell activation, cell survival and proliferation, but the full range of its functions remains to be elucidated. The receptor has an extracellular immunoglobulin (Ig)-like domain homologous to those in Fcα/μR and pIgR, but unlike these two other IgM receptors which also bind IgA, FcμR exhibits a binding specificity for only IgM-Fc. Previous st...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - August 25, 2019 Category: Biochemistry Source Type: research

Early mechanisms of amyloid fibril nucleation in model and disease-related proteins
Publication date: Available online 19 August 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Bertrand Morel, Francisco Conejero-LaraABSTRACTProtein amyloid aggregation is a hallmark in neuropathologies and other diseases of tremendous impact such as Alzheimer’s or Parkinson’s diseases. During the last decade, it has become increasingly evident that neuronal death is mainly induced by proteinaceous oligomers rather than the mature amyloid fibrils. Therefore, the earliest molecular events occurring during the amyloid aggregation cascade represent a growing interest of study.Important b...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - August 20, 2019 Category: Biochemistry Source Type: research