Biochimica et Biophysica Acta (BBA) Proteins and Proteomics This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases
Publication date: Available online 16 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Rhys Cutlan, Simone De Rose, Michail N. Isupov, Jennifer A. Littlechild, Nicholas J. HarmerAbstractBiocatalysis, the use of enzymes in chemical transformations, is an important green chemistry tool. Cascade reactions combine different enzyme activities in a sequential set of reactions. Cascades can occur within a living (usually bacterial) cell; in vitro in ‘one pot’ systems where the desired enzymes are mixed together to carry out the multi-enzyme reaction; or using microfluidic systems. M...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research

Quantitative proteomics indicate a strong correlation of mitotic phospho-/dephosphorylation with non-structured regions of substrates
In this study, we examined structural properties of mitotic phosphosites and neighboring residues to understand the role of heavy phosphorylation in non-structured domains. Quantitative mass spectrometry analysis of mitosis-arrested and non-arrested HeLa cells revealed>4100 and > 2200 residues either significantly phosphorylated or dephosphorylated, respectively, at mitotic entry. The calculated disorder scores of amino acid sequences of neighboring individual phosphosites revealed that>70% of dephosphorylated phosphosites exist in disordered regions, whereas 50% of phosphorylated sites exist in non-structured domains....
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 13, 2019 Category: Biochemistry Source Type: research

Thermophilic nucleoside phosphorylases: Their properties, characteristics and applications
Publication date: Available online 2 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Sarah Kamel, Isabel Thiele, Peter Neubauer, Anke WagnerAbstractNucleoside phosphorylases catalyze the reversible phosphorolysis of pyrimidine and purine nucleosides in the presence of phosphate. They are valuable catalysts in the synthesis of nucleosides and their analogues, which are often used as pharmaceuticals or their precursors. Thermostable nucleoside phosphorylases are promising biocatalysts, as they withstand harsh reaction conditions such as high pH or the addition of organic solvents....
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 3, 2019 Category: Biochemistry Source Type: research

Photochemical regeneration of flavoenzymes – An Old Yellow Enzyme case-study
Publication date: Available online 31 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): M.C.R. Rauch, M. Pesic, M.M.E. Huijbers, M. Pabst, C.E. Paul, M. Pesic, I.W.C.E. Arends, F. HollmannAbstractDirect, NAD(P)H-independent regeneration of Old Yellow Enzymes represents an interesting approach for simplified reaction schemes for the stereoselective reduction of conjugated C=C-double bonds. Simply by illuminating the reaction mixtures with blue light in the presence of sacrificial electron donors enables to circumvent the costly and unstable nicotinamide cofactors and a corresponding...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 1, 2019 Category: Biochemistry Source Type: research

Chemoenzymatic synthesis of ultralow and low-molecular weight heparins
Publication date: Available online 31 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Ting Wang, Li Liu, Josef VoglmeirAbstractHeparin is a naturally occurring glycosaminoglycan isolated from animal tissues and is medically used as an anticoagulant drug. Adulteration attempts of isolated heparin with chondroitin sulfate in the past resulted in great safety concerns. Also, increasing demands on batch-to-batch homogeneity for better evaluation and control of its pharmacodynamic and pharmacokinetic properties kindled the development of synthetic routes for the production of heparin ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 1, 2019 Category: Biochemistry Source Type: research

Ubiquitin folds via a flip-twist-lock mechanism
Publication date: Available online 30 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Manoj Mandal, Atanu Das, Chaitali MukhopadhyayAbstractTo perform specific functional activities, the majority of proteins should fold into their distinct three-dimensional conformations. However, the biologically active conformation of a protein is generally found to be marginally stable than the other conformations that the chain can adopt. How a protein finds its native conformation from its post-synthesis unfolded structure in a complex conformational landscape is the unsolved question that s...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Sequential oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid by an evolved aryl-alcohol oxidase
Publication date: Available online 30 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Javier Viña-Gonzalez, Angel T. Martinez, Victor Guallar, Miguel AlcaldeAbstractFuran-2,5-dicarboxylic acid (FDCA) is a building block of biodegradable plastics that can be used to replace those derived from fossil carbon sources. In recent years, much interest has focused on the synthesis of FDCA from the bio-based 5-hydroxymethylfurfural (HMF) through a cascade of enzyme reactions. Aryl-alcohol oxidase (AAO) and 5-hydroxymethylfurfural oxidase (HMFO) are glucose-methanol-choline flavoenzymes t...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Use of nucleoside phosphorylases for the preparation of 5-modified pyrimidine ribonucleosides
Publication date: Available online 30 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Cyril S. Alexeev, Mikhail S. Drenichev, Evgeniya O. Dorinova, Roman S. Esipov, Irina V. Kulikova, Sergey N. MikhailovAbstractEnzymatic transglycosylation, a transfer of the carbohydrate moiety from one heterocyclic base to another, is catalyzed by nucleoside phosphorylases (NPs) and is being actively developed and applied for the synthesis of biologically important nucleosides. Here, we report an efficient one-step synthesis of 5-substitited pyrimidine ribonucleosides starting from 7-methylguano...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Roles of the N-terminal domain and remote substrate binding subsites in activity of the debranching barley limit dextrinase
Publication date: Available online 30 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Susan Andersen, Birte Svensson, Marie Sofie MøllerAbstractBarley limit dextrinase (HvLD) of glycoside hydrolase family 13 is the sole enzyme hydrolysing α-1,6-glucosidic linkages from starch in the germinating seed. Surprisingly, HvLD shows 150- and 7-fold higher activity towards pullulan and β-limit dextrin, respectively, than amylopectin. This is investigated by mutational analysis of residues in the N-terminal CBM-21-like domain (Ser14Arg, His108Arg, Ser14Arg/His108Arg) and at the outer su...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Biochemical and biophysical characterization of the smallest pyruvate kinase from Entamoeba histolytica
Publication date: Available online 30 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Poonam Kumari, Danish Idrees, Pragyan Parimita Rath, Ramachandran Vijayan, Samudrala GourinathAbstractEntamoeba histolytica infection is highly prevalent in developing countries across the globe. The ATP synthesis in this pathogen is solely dependent on the glycolysis pathway where pyruvate kinase (Pyk) catalyzes the final reaction. Here, we have cloned, overexpressed and purified the pyruvate kinase (EhPyk) from E. histolytica. EhPyk is the shortest currently known Pyk till date as it contains ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Molecular characterization of an aggregation-prone variant of alpha-synuclein used to model synucleinopathies
Publication date: Available online 30 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Caterina Masaracchia, Annekatrin König, Ariel A. Valiente-Gabioud, Pablo Peralta, Filippo Favretto, Timo Strohäker, Diana F. Lázaro, Markus Zweckstetter, Claudio O. Fernandez, Tiago F. OuteiroAbstractThe misfolding and aggregation of alpha-synuclein (aSyn) are thought to be central events in synucleinopathies. The physiological function of aSyn has been related to vesicle binding and trafficking, but the precise molecular mechanisms leading to aSyn pathogenicity are still obscure. In cell mod...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Quantitative proteomics indicate a strong correlation of mitotic phospho−/dephosphorylation with non-structured regions of substrates
In this study, we examined structural properties of mitotic phosphosites and neighboring residues to understand the role of heavy phosphorylation in non-structured domains. Quantitative mass spectrometry analysis of mitosis-arrested and non-arrested HeLa cells revealed>4100 and > 2200 residues either significantly phosphorylated or dephosphorylated, respectively, at mitotic entry. The calculated disorder scores of amino acid sequences of neighboring individual phosphosites revealed that>70% of dephosphorylated phosphosites exist in disordered regions, whereas 50% of phosphorylated sites exist in non-structured domains....
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

On the catalytic mechanism of bacteriophage endolysins: Opportunities for engineering
Publication date: Available online 31 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Michael J. Love, Gayan S. Abeysekera, Andrew C. Muscroft-Taylor, Craig Billington, Renwick C.J. DobsonAbstractBacteriophage endolysins have the potential to be a long-term antibacterial replacement for antibiotics. The exogenous application of endolysins on some bacteria results in rapid cell lysis. The prospects for endolysins are furthered by the ability to engineer them; novel endolysins can be developed with optimised stability, specificity, and lytic function. But the success of endolysin e...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Characterization of alkaline phosphatase PhoK from Sphingomonas sp. BSAR-1 for phosphate monoester synthesis and hydrolysis
Publication date: Available online 31 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Michael Lukesch, Gábor Tasnádi, Klaus Ditrich, Mélanie Hall, Kurt FaberAbstractThe biocatalytic activity of a so far underexploited alkaline phosphatase, PhoK from Sphingomonas sp. BSAR-1, was extensively studied in transphosphorylation and hydrolysis reactions. The use of high-energy phosphate donors and oligophosphates as suitable phosphate donor was evaluated, as well as the hydrolytic activity on a variety of phosphate monoesters. While substrates bearing free hydroxy group displayed only...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 31, 2019 Category: Biochemistry Source Type: research

Purification and characterization of two forms of the homologously expressed lytic polysaccharide monooxygenase (PvLPMO9A) from Penicillium verruculosum
Publication date: Available online 28 October 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Margarita V. Semenova, Alexander V. Gusakov, Vadim D. Telitsin, Aleksandra M. Rozhkova, Elena G. Kondratyeva, Arkady P. SinitsynAbstractTwo forms of C1/C4-oxidizing lytic polysaccharide monooxygenase (PvLPMO9A) from Penicillium verruculosum (Talaromyces verruculosus) homologously expressed in P. verruculosum B1-537 auxotrophic strain were isolated in a homogeneous state using two-stage chromatography. The PvLPMO9A-hm form represented a full-size enzyme encoded by the intact lpmo1 gene, while the...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - October 29, 2019 Category: Biochemistry Source Type: research