Biochimica et Biophysica Acta (BBA) Proteins and Proteomics This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Editorial Board
Publication date: January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1868, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 29, 2019 Category: Biochemistry Source Type: research

Reviewer Acknowledgement
Publication date: January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1868, Issue 1Author(s): (Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics)
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 29, 2019 Category: Biochemistry Source Type: research

Characterization of pyranose oxidase variants for bioelectrocatalytic applications
In this study, enzyme variants with improved electron acceptor turnover and reduced oxygen turnover were characterized as potential anode biocatalysts. Pre-steady-state kinetics of the oxidative half-reaction of ToPOx variants T166R, Q448H, L545C, and L547R with these alternative electron acceptors were evaluated using stopped-flow spectrophotometry. Higher kinetic constants were observed as compared to the wild-type ToPOx for some of the variants. Subsequently, the variants were immobilized on glassy carbon electrodes. Cyclic voltammetry measurements were performed to measure the electrochemical responses of these variant...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 28, 2019 Category: Biochemistry Source Type: research

On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective
Publication date: Available online 25 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Juan M. Bolivar, Bernd NidetzkyAbstractThe integration of enzymes with solid materials is important in many biotechnological applications, including the use of immobilized enzymes for biocatalytic synthesis. The development of functional enzyme-material composites is restrained by the lack of molecular-level insight into the behavior of enzymes in confined, surface-near environments. Here, we review recent advances in surface-sensitive spectroscopic techniques that push boundaries for the deter...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 26, 2019 Category: Biochemistry Source Type: research

New insights into human endometrial aminopeptidases in both implantation and menstruation
Publication date: Available online 23 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Shigehiko Mizutani, Kunio Matsumoto, Yukio Kato, Eita Mizutani, Hidesuke Mizutani, Akira Iwase, Kiyosumi ShibataAbstractThe endometrium cycle involves proliferation of endometrial epithelial cells in preparation for implantation of fertilized ovum. With ovulation, the endometrium secretes nutrients such as peptides and amino acids into the endometrial cavity. The histological evidence of ovulation in normal menstrual cycle includes subnuclear glycogen vacuoles surrounded by placental leucine am...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 23, 2019 Category: Biochemistry Source Type: research

The substrate of the glucose-6-phosphate dehydrogenase of Pseudomonas aeruginosa provides structural stability
Publication date: Available online 22 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Edaena Benítez-Rangel, Annia Rodríguez-Hernández, Roberto Velasco-GarcíaAbstractIn general, eukaryotic glucose-6-phosphate dehydrogenases (G6PDHs) are structurally stabilized by NADP+. Here we show by spectrofluorometric analysis, thermal and urea denaturation, and trypsin proteolysis, that a different mechanism stabilizes the enzyme from Pseudomonas aeruginosa (PaG6PDH) (EC 1.1.1.363). The spectrofluorometric analysis of the emission of 8-anilino-1-naphthalenesulfonic acid (ANS) indicates ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 22, 2019 Category: Biochemistry Source Type: research

Protein knots provide mechano-resilience to an AAA+ protease-mediated proteolysis with profound ATP energy expenses
In this study, we directly monitored the ClpXP-mediated degradation of UCH variants by electrophoresis and quantitative imaging analyses. We demonstrated that untying of the 52 knot in UCHL1 via N-terminal truncation (UCHL1Δ11) significantly reduces its mechanostability. We further quantified the ATP expenditures of degrading different UCH variants by ClpXP. The unknotted UCHL1Δ11 underwent accelerated ClpXP-dependent proteolysis, with a 30-fold reduction in ATP consumption compared to the knotted wild type. Unlike all other known ClpXP substrates, UCHL5, which is the most resilient substrate known to date, significantly...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 20, 2019 Category: Biochemistry Source Type: research

Metagenomics: Is it a powerful tool to obtain lipases for application in biocatalysis?
Publication date: Available online 20 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Janaina Marques de Almeida, Robson Carlos Alnoch, Emanuel Maltempi de Souza, David Alexander Mitchell, Nadia KriegerAbstractIn recent years, metagenomic strategies have been widely used to isolate and identify new enzymes from uncultivable components of microbial communities. Among these enzymes, various lipases have been obtained from metagenomic libraries from different environments and characterized. Although many of these lipases have characteristics that could make them interesting for app...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 20, 2019 Category: Biochemistry Source Type: research

Energy migration captures membrane-induced oligomerization of the prion protein
Publication date: Available online 15 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Aishwarya Agarwal, Debapriya Das, Tisya Banerjee, Samrat MukhopadhyayAbstractExcitation energy migration via homo-Förster resonance energy transfer (homo-FRET) can serve as an intermolecular proximity ruler within complex biomolecular assemblies. Here we present a unique case to demonstrate that energy migration can be a novel and sensitive readout to capture the membrane-mediated misfolding and oligomerization of the human prion protein (PrP), which is known to undergo an aberrant conformatio...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research

Rat cathepsin K: Enzymatic specificity and regulation of its collagenolytic activity
Publication date: Available online 15 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Fabien Lecaille, Thibault Chazeirat, Krzysztof K. Bojarski, Justine Renault, Ahlame Saidi, V. Gangadhara N.V. Prasad, Sergey Samsonov, Gilles LalmanachAbstractHuman cathepsin K (hCatK), which is highly expressed in osteoclasts, has the noteworthy ability to cleave type I and II collagens in their helical domain. Its collagenase potency depends strictly on the formation of an oligomeric complex with chondroitin 4-sulfate (C4-S). Accordingly, hCatK is a pivotal protease involved in bone resorptio...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research

Unbiased libraries in protein directed evolution
Publication date: Available online 15 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Victor Sayous, Paul Lubrano, Yanyan Li, Carlos G. Acevedo-RochaAbstractDirected evolution is a powerful approach to study the molecular basis of protein evolution and to engineer proteins for a wide range of applications in synthetic organic chemistry and biotechnology. There are many methods based on random or focused mutagenesis to engineer successfully any protein trait. Focused approaches such as site-directed and saturation mutagenesis have become methods of choice for improving protein ac...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research

A novel enzymatic tool for transferring GalNAc moiety onto challenging acceptors
We present here a novel production method of this enzyme in the eukaryotic host of Pichia pastoris, followed by elegant one-step purification to homogeneity. The resulting recombinant enzyme has improved biochemical and catalytic properties compared to the fungal wild type. Its good production yield (11 mg/400 mL cultivation media) greatly expands the scope of synthetic applications. We further demonstrate the synthetic utility and broad acceptor specificity of recombinant PoHex in the glycosylation of a series of challenging acceptors with varying structural architectures, namely secondary and tertiary hydroxyl, aldox...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research

Identification and application of threonine aldolase for synthesis of valuable α-amino, β-hydroxy-building blocks
Publication date: Available online 15 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Mathieu Ligibel, Charles Moore, Robert Bruccoleri, Radka SnajdrovaAbstractChiral β-hydroxy α-amino acid structural motifs are interesting and common synthons present in multiple APIs and drug candidates. To access these chiral building blocks either multistep chemical syntheses are required or the application of threonine aldolases, which catalyze aldol reactions between an aldehyde and glycine. Bioinformatics tools have been utilized to identify the gene encoding threonine aldolase from Vanr...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research

Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester-type plastics
Publication date: Available online 16 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Aneta K. Urbanek, Aleksandra M. Mirończuk, Alberto García-Martín, Ana Saborido, Isabel de la Mata, Miguel ArroyoAbstractApplication of polyester-degrading enzymes should be considered as an eco-friendly alternative to chemical recycling due to the huge plastic waste disposal nowadays. Many hydrolases from several fungi and bacteria have been discovered and successfully evaluated for their activity towards different aliphatic polyesters (PHA, PBS, PBSA, PCL, PLA), aromatic polyesters (PET, PB...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research

Xylanases from marine microorganisms: A brief overview on scope, sources, features and potential applications
Publication date: Available online 16 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Fatemeh Izadpanah Qeshmi, Ahmad Homaei, Pedro Fernandes, Roohullah Hemmati, Bauke W. Dijkstra, Khosro KhajehAbstractGlobal economic growth often leads to depletion of raw materials and generation of greenhouse gases, as industry manufactures goods at ever increasing levels to keep up with the demand. The currently implemented production processes mostly rely on non-renewable resources, they suffer from high energy consumption, and generate waste that often has a negative environmental impact. E...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - November 17, 2019 Category: Biochemistry Source Type: research