2,4 ‑Di‑tert‑butylphenol, a potential HDAC6 inhibitor, induces senescence and mitotic catastrophe in human gastric adenocarcinoma AGS cells
Publication date: May 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 5 Author(s): Yeon Woo Song, Yoongho Lim, Somi Kim Cho The natural product 2,4‑di‑tert‑butylphenol (DTBP) has a wide spectrum of biological functions, including anticancer activities, although the underlying mechanisms are poorly understood. Here, we found that DTBP induces senescence in human gastric adenocarcinoma AGS cells as evidenced by upregulation of p21 and Rb and increased β‑galactosidase activity. DTBP also induces mitotic catastrophe and generates multinucleated cells, which is accompa...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 23, 2018 Category: Molecular Biology Source Type: research

Similarities and differences between the responses induced in human phagocytes through activation of the medium chain fatty acid receptor GPR84 and the short chain fatty acid receptor FFA2R
In this study, we have characterized the GPR84 activation profile and regulation mechanism in human phagocytes, using two recently developed small molecules that specifically target GPR84 agonistically (ZQ16) and antagonistically (GLPG1205), respectively. Compared to our earlier characterization of the short chain fatty acid receptor FFA2R which is functionally expressed in neutrophils but not in monocytes, GPR84 is expressed in both cell types and in monocyte-derived macrophages. In neutrophils, the GPR84 agonist had an activation profile very similar to that of FFA2R. The GPR84-mediated superoxide release was low in na&i...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 22, 2018 Category: Molecular Biology Source Type: research

RACK1/TRAF2 regulation of modulator of apoptosis (MOAP-1)
In this study, we demonstrate robust association with RACK1, a versatile scaffolding protein that responds to activation of protein kinase C. Furthermore, we can demonstrate that RACK1 functions to bring the E3 ligase, TRAF2, to MOAP-1 in order to undergo a K63-dependent ubiquitination. Furthermore, RACK1 associates with MOAP-1 via electrostatic associations similar to those observed between MOAP-1/RASSF1A and MOAP-1/TNF-R1. These events illustrate the complex nature of MOAP-1 regulation and characterizes the important role of the scaffolding protein, RACK1, in influencing MOAP-1 biology. (Source: Biochimica et Biophysica ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 19, 2018 Category: Molecular Biology Source Type: research

Low shear stress induces vascular eNOS uncoupling via autophagy-mediated eNOS phosphorylation
Publication date: Available online 18 February 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Jun-Xia Zhang, Xin-Liang Qu, Peng Chu, Du-Jiang Xie, Lin-Lin Zhu, Yue-Lin Chao, Li Li, Jun-Jie Zhang, Shao-Liang Chen Uncoupled endothelial nitric oxide synthase (eNOS) produces O2 − instead of nitric oxide (NO). Earlier, we reported rapamycin, an autophagy inducer and inhibitor of cellular proliferation, attenuated low shear stress (SS) induced O2 − production. Nevertheless, it is unclear whether autophagy plays a critical role in the regulation of eNOS uncoupling. Therefore, thi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 18, 2018 Category: Molecular Biology Source Type: research

Reciprocal regulation of the Cadherin-11/Stat3 axis by caveolin-1 in mouse fibroblasts and lung carcinoma cells
Publication date: Available online 16 February 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): M. Geletu, R. Mohan, R. Arulanandam, A. Berger-Becvar, I.R. Nabi, P.T. Gunning, L. Raptis Caveolin-1 (Cav1) is an integral plasma membrane protein and a complex regulator of signal transduction. The Signal Transducer and Activator of Transcription-3 (Stat3) is activated by a number of receptor and non-receptor tyrosine kinases and is positively implicated in cancer. Despite extensive efforts, the relationship between Cav1 and Stat3 has been a matter of controversy. We previously demonstrated ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 16, 2018 Category: Molecular Biology Source Type: research

Protein Kinase C- ζ stimulates colorectal cancer cell carcinogenesis via PKC-ζ/Rac1/Pak1/β-Catenin signaling cascade
In this study, the role of atypical Protein Kinase C (aPKC) on CRC was investigated by using two inhibitors of that protein class: 1) ζ-Stat (8-hydroxynaphthalene-1,3,6-trisulfonic acid) is a specific inhibitor of PKC-ζ and 2) ICA-I 5-amino-1-(2,3-dihydroxy-4-hydroxymethyl)cyclopentyl)-1H-imidazole-4-carboxamide) is a specific inhibitor of PKC-ι. The cell lines tested were CCD18CO normal colon epithelial and LOVO metastatic CRC cells. The inhibition of aPKCs did not bring any significant toxicity on CCD18CO normal colon cell line. Although PKC-ι is an oncogene in many cancers, we found the overexpressio...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 10, 2018 Category: Molecular Biology Source Type: research

Nontypeable Haemophilus influenzae DNA stimulates type I interferon expression via STING signaling pathway
In this study, we found expression of IFN-β and IFN stimulated gene CXCL10 in host cells was forcefully elevated after treating with NTHI and NTHI DNA. Interestingly, we tested increased level of STING in NTHI infected mice lung. Meanwhile, STING expression in lung of mimic COPD murine model was higher than healthy mice after NTHI infection. Importantly, knockout of STING or overexpression of STING, TBK1 and IRF3 respectively impaired or enhanced IFN-β and CXCL10 expression during treating with NTHI and NTHI DNA. NTHI and NTHI DNA-induced I-IFN response appeared to be mediated by cGAS. Collectively, we suggested ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 5, 2018 Category: Molecular Biology Source Type: research

Ras-Raf-MAPK signaling promotes nuclear localization of FOXA transcription factor SGF1 via Ser91 phosphorylation
In conclusion, Ras-Raf-MAPK signaling promotes SGF1 nuclear localization for transactivation via Ser91 phosphorylation in silkworms, showing that FOXA transcription factors are regulated via MAPK phosphorylation in animals. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 4, 2018 Category: Molecular Biology Source Type: research

GRP78 protects CHO cells from ribosylation
In this study, we found that cellular AGEs accumulate in Chinese hamster ovary (CHO) cells with increased Rib concentration and administration time. Mass spectrum analysis of isolated AGE-modified proteins from cell lysates showed that glucose-regulated protein 78 kD (GRP78) is one of the main ribosylated proteins. Co-immunoprecipitation assays further confirmed the interaction between AGEs and GRP78. Compared with d-glucose (Glc), Rib produced much more AGEs in cells. In kinetic studies, the first order rate constant of LDH released from CHO cells incubated with Rib was nearly 8-fold higher than that of Glc, suggesting th...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 3, 2018 Category: Molecular Biology Source Type: research

In vitro identification of mitochondrial oxidative stress production by time-resolved fluorescence imaging of glioma cells
Publication date: Available online 2 February 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Silvia Tomkova, Matus Misuth, Lenka Lenkavska, Pavol Miskovsky, Veronika Huntosova Oxidative phosphorylation and glycolysis are important features, by which cells could bypass oxidative stress. The level of oxidative stress, and the ability of cells to promote oxidative phosphorylation or glycolysis, significantly determined proliferation or cell demise. In the present work, we have employed selective mitochondrial probe MitoTracker™ Orange CMTM/Ros (MTO) to estimate the level of oxidati...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 2, 2018 Category: Molecular Biology Source Type: research

The epigenetic factor KDM2B regulates cell adhesion, small rho GTPases, actin cytoskeleton and migration in prostate cancer cells
We report here that KDM2B is functionally expressed in DU-145 prostate cancer cells, activated by FGF-2 and regulates EZH2. KDM2B knockdown induced potent up-regulation of gene transcription and protein expression of the epithelial markers E-cadherin and ZO-1, while KDM2B overexpression down-regulated the levels of both markers, suggesting control of cell adhesion by KDM2B. RhoA and RhoB protein expression and activity were diminished upon KDM2B-knockdown and upregulated in KDM2B-overexpressing cell clones. In accordance, actin reorganization with formation of stress fibers became evident in KDM2B-overexpressing cells and ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 2, 2018 Category: Molecular Biology Source Type: research

Heparan sulfate mediates cell uptake of αB-crystallin fused to the glycoprotein C cell penetration peptide
Publication date: Available online 31 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Steven Droho, Mitchell E. Keener, Niklaus H. Mueller (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 1, 2018 Category: Molecular Biology Source Type: research

Junctional adhesion molecule C (JAM-C) dimerization aids cancer cell migration and metastasis
Publication date: Available online 31 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Sarah Garrido-Urbani, Alain Vonlaufen, Jimmy Stalin, Maria De Grandis, Patricia Ropraz, Stéphane Jemelin, Florence Bardin, Holger Scheib, Michel Aurrand-Lions, Beat A. Imhof Most cancer deaths result from metastasis, which is the dissemination of cells from a primary tumor to distant organs. Metastasis involves changes to molecules that are essential for tumor cell adhesion to the extracellular matrix and to endothelial cells. Junctional Adhesion Molecule C (JAM-C) localizes at intercel...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 31, 2018 Category: Molecular Biology Source Type: research

Corrigendum to “Distorted leukocyte migration, angiogenesis, wound repair and metastasis in Tspan8 and Tspan8/CD151 double knockout mice indicate complementary activities of Tspan8 and CD51” [Biochim. Biophys. Acta 1865(2) (2018) 379–391]
Publication date: Available online 30 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Kun Zhao, Ulrike Erb, Thilo Hackert, Margot Zöller, Shijing Yue (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 30, 2018 Category: Molecular Biology Source Type: research

Helicobacter pylori induces direct activation of the lymphotoxin beta receptor and non-canonical nuclear factor-kappa B signaling
Publication date: Available online 9 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Michael Hartmut Feige, Michael Vieth, Olga Sokolova, Christian Täger, Michael Naumann The pathogen Helicobacter pylori, which infects half of the world's population, is a major risk factor for the development of gastric diseases including chronic gastritis and gastric cancer. Among H. pylori's virulence factors is the cytotoxin-associated gene pathogenicity island (cagPAI), which encodes for a type IV secretion system (T4SS). The T4SS induces fast canonical nuclear factor-kappa B (NF-&kapp...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 22, 2018 Category: Molecular Biology Source Type: research

Corrigendum to “Bcl xL deamidation and cancer: Charting the fame trajectories of legitimate child and hidden siblings.” [Biochim. Biophys. Acta 1864/10 (2017) 1734–1745]
Publication date: Available online 10 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Florian Beaumatin, Mohamad El Dhaybi, Claude Bobo, Mireille Verdier, Muriel Priault (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 22, 2018 Category: Molecular Biology Source Type: research

Retinoic acid promotes stem cell differentiation and embryonic development by transcriptionally activating CFTR
Publication date: Available online 9 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Xiaofeng Li, Kin Lam Fok, Jinghui Guo, Yan Wang, Zhenqing Liu, Ziyi Chen, Chengdong Wang, Ye Chun Ruan, Sidney Siubun Yu, Hui Zhao, Ji Wu, Xiaohua Jiang, Hsiao Chang Chan Retinoic acid (RA) plays a pivotal role in many cellular processes; however, the signaling mechanisms mediating the effect of RA are not fully understood. Here, we show that RA transcriptionally upregulates cystic fibrosis transmembrane conductance regulator (Cftr) by promoting the direct binding of its receptor RARα to ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 9, 2018 Category: Molecular Biology Source Type: research

Insulin-induced translocation of IR to the nucleus in insulin responsive cells requires a nuclear translocation sequence
In this study we explored the mechanism by which insulin induces IR translocation to the cell nucleus. Experiments were performed cultured L6 myoblasts, AML liver cells and 3T3-L1 adipocytes. Insulin treatment induced a rapid increase in nuclear IR protein levels within 2 to 5min. Treatment with WGA, an inhibitor of nuclear import, reduced insulin-induced increases nuclear IR protein; IR was, however, translocated to a perinuclear location. Bioinformatics tools predicted a potential nuclear localization sequence (NLS) on IR. Immunofluorescence staining showed that a point mutation on the predicted NLS blocked insulin-induc...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 6, 2018 Category: Molecular Biology Source Type: research

Meiotic development initiation in the fungus Podospora anserina requires the peroxisome receptor export machinery
Publication date: Available online 4 January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Fernando Suaste-Olmos, Claudia Zirión-Martínez, Harumi Takano-Rojas, Leonardo Peraza-Reyes Peroxisomes are versatile organelles essential for diverse developmental processes. One such process is the meiotic development of Podospora anserina. In this fungus, absence of the docking peroxin PEX13, the RING-finger complex peroxins, or the PTS2 co-receptor PEX20 blocks sexual development before meiocyte formation. However, this defect is not seen in the absence of the receptors PEX5 an...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 4, 2018 Category: Molecular Biology Source Type: research

TRAIL induces apoptosis but not necroptosis in colorectal and pancreatic cancer cells preferentially via the TRAIL-R2/DR5 receptor
Publication date: March 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 3 Author(s): Zuzana Nahacka, Jan Svadlenka, Martin Peterka, Marie Ksandrova, Simona Benesova, Jiri Neuzil, Ladislav Andera Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytokine that can trigger apoptosis in many types of human cancer cells via engagement of its two pro-apoptotic receptors TRAIL-R1 (DR4) and TRAIL-R2 (DR5). TRAIL can also activate several other signaling pathways such as activation of stress kinases, canonical NF-κB signaling and necroptosis. Though both recept...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 4, 2018 Category: Molecular Biology Source Type: research

The putative flippase Apt1 is required for intracellular membrane architecture and biosynthesis of polysaccharide and lipids in Cryptococcus neoformans
In this study, we analyzed the role of C. neoformans Apt1 in intracellular membrane architecture and synthesis of polysaccharide and lipids. Analysis of wild type (WT), apt1Δ (mutant) and apt1Δ::APT1 (complemented) strains by transmission electron microscopy revealed that deletion of APT1 resulted in the formation of irregular vacuoles. Disorganization of vacuolar membranes in apt1Δ cells was accompanied by a significant increase in the amounts of intra-vacuolar and pigment-containing vesicles. Quantitative immunogold labeling of C. neoformans cells with a monoclonal antibody raised to a major capsular co...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 29, 2017 Category: Molecular Biology Source Type: research

TRAIL-induces apoptosis but not necroptosis in colorectal and pancreatic cancer cells preferentially via the TRAIL-R2/DR5 receptor
Publication date: Available online 24 December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Zuzana Nahacka, Jan Svadlenka, Martin Peterka, Marie Ksandrova, Simona Benesova, Jiri Neuzil, Ladislav Andera Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytokine that can trigger apoptosis in many types of human cancer cells via engagement of its two pro-apoptotic receptors TRAIL-R1 (DR4) and TRAIL-R2 (DR5). TRAIL can also activate several other signaling pathways such as activation of stress kinases, canonical NF-κB signaling and necroptosis. Though both recep...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 24, 2017 Category: Molecular Biology Source Type: research

Calmodulin as a protein linker and a regulator of adaptor/scaffold proteins
Publication date: Available online 13 December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Antonio Villalobo, Hiroaki Ishida, Hans J. Vogel, Martin W. Berchtold Calmodulin (CaM) is a universal regulator for a huge number of proteins in all eukaryotic cells. Best known is its function as a calcium-dependent modulator of the activity of enzymes, such as protein kinases and phosphatases, as well as other signaling proteins including membrane receptors, channels and structural proteins. However, less well known is the fact that CaM can also function as a Ca2+-dependent adaptor protein,...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 13, 2017 Category: Molecular Biology Source Type: research

Elevation of sensitivity to anticancer agents of human lung adenocarcinoma A549 cells by knockdown of claudin-2 expression in monolayer and spheroid culture models
Publication date: Available online 13 December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Ryohei Maruhashi, Risa Akizuki, Tomonari Sato, Toshiyuki Matsunaga, Satoshi Endo, Masahiko Yamaguchi, Yasuhiro Yamazaki, Hideki Sakai, Akira Ikari Claudins, tight junctional proteins, regulate the paracellular permeability of ions and small molecules. Claudin-2 is highly expressed in human lung adenocarcinoma cells and is involved in the up-regulation of cell proliferation. However, the effect of claudin-2 on cellular sensitivity to anticancer agents has not been clarified. The cytotoxicity o...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 13, 2017 Category: Molecular Biology Source Type: research

The SNP rs4252548 (R112H) which is associated with reduced human height compromises the stability of IL-11
In this study, we show by molecular replacement that Arg-112 does not participate in binding of IL-11 to its receptors IL-11R and glycoprotein 130 (gp130). Recombinant IL-11 R112H expressed in E. coli displays a correct four-helix-bundle folding topology, and binds with similar affinity to IL-11R and the IL-11/IL-11R/gp130 complex. IL-11 R112H induces cell proliferation and phosphorylation of the downstream transcription factor STAT3 indistinguishable from IL-11. However, IL-11 R112H fails to support the survival of osteoclast progenitor cells and is less thermally stable, which is caused by the loss of the positive charge...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 10, 2017 Category: Molecular Biology Source Type: research

PUMA dependent mitophagy by Abrus agglutinin contributes to apoptosis through ceramide generation
In this study, we report mitophagy inducing potential of PUMA triggered by phytolectin Abrus agglutinin (AGG) in U87MG glioblastoma cells and established AGG-induced ceramide acts as the chief mediator of mitophagy dependent cell death through activation of both mitochondrial ROS as well as ER stress. Importantly, AGG upregulates PUMA expression in U87MG cells with the generation of dysfunctional mitochondria, and gain and loss of function of PUMA is shown to alter mitophagy induction. At the molecular level, our study identified that the LC3 interacting region (LIR) located at the C-terminal end of PUMA interacts with LC3...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 8, 2017 Category: Molecular Biology Source Type: research

Fine-tuning of store-operated calcium entry by fast and slow Ca2+-dependent inactivation: Involvement of SARAF
Publication date: Available online 6 December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Isaac Jardín, Letizia Albarran, Ginés M. Salido, Jose J. López, Stewart O. Sage, Juan A. Rosado Abstract Store-operated Ca2+ entry (SOCE) is a functionally relevant mechanism for Ca2+ influx present in electrically excitable and non-excitable cells. Regulation of Ca2+ entry through store-operated channels is essential to maintain an appropriate intracellular Ca2+ homeostasis and prevent cell damage. Calcium-release activated channels exhibit Ca2+-dependent inactivation med...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 6, 2017 Category: Molecular Biology Source Type: research

Vesicular transport protein Arf6 modulates cytoskeleton dynamics for polar body extrusion in mouse oocyte meiosis
Publication date: Available online 6 December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Xing Duan, Hao-Lin Zhang, Meng-Hao Pan, Yu Zhang, Shao-Chen Sun Arf6 (ADP-ribosylation factor 6) is known to play important roles in membrane dynamics through the regulation of actin filament reorganization for multiple cellular processes such as cytokinesis, phagocytosis, cell migration and tumor cell invasion. However, the functions of Arf6 in mammalian oocyte meiosis have not been clarified. In present study we showed that Arf6 expressed in mouse oocytes and was mainly distributed around th...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 6, 2017 Category: Molecular Biology Source Type: research

Heterochromatin protein (HP)1 γ is not only in the nucleus but also in the cytoplasm interacting with actin in both cell compartments
Publication date: Available online 6 December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Nancy L. Charó, Natalia M. Galigniana, Graciela Piwien-Pilipuk Confocal and electron microscopy images, and WB analysis of cellular fractions revealed that HP1γ is in the nucleus but also in the cytoplasm of C2C12 myoblasts, myotubes, skeletal and cardiac muscles, N2a, HeLa and HEK293T cells. Signal specificity was tested with different antibodies and by HP1γ knockdown. Leptomycin B treatment of myoblasts increased nuclear HP1γ, suggesting that its nuclear export is Cr...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 6, 2017 Category: Molecular Biology Source Type: research

S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils
Publication date: Available online 2 December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Sheela Nagarkoti, Megha Dubey, Deepika Awasthi, Vikas Kumar, Tulika Chandra, Sachin Kumar, Madhu Dikshit Post-translational modifications (PTMs) induced conformational changes of proteins can cause their activation or inactivation. Neutrophils clear pathogen through phagocytosis and oxidative burst generation, while participate in inflammation through sustained and uncontrolled generation of ROS. In activated PMNs, cytosolic NOX-2 subunit p47phox following phosphorylation interacts with p67pho...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 2, 2017 Category: Molecular Biology Source Type: research

Control of nuclear β-dystroglycan content is crucial for the maintenance of nuclear envelope integrity and function
Publication date: February 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 2 Author(s): Griselda Vélez-Aguilera, Juan de Dios Gómez-López, Guadalupe E. Jiménez-Gutiérrez, Alejandra Vásquez-Limeta, Marco S. Laredo-Cisneros, Pablo Gómez, Steve J. Winder, Bulmaro Cisneros β-Dystroglycan (β-DG) is a plasma membrane protein that has ability to target to the nuclear envelope (NE) to maintain nuclear architecture. Nevertheless, mechanisms controlling β-DG nuclear localization and the physiological consequences of a failu...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 1, 2017 Category: Molecular Biology Source Type: research

Soft drug-resistant ovarian cancer cells migrate via two distinct mechanisms utilizing myosin II-based contractility
Publication date: February 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 2 Author(s): Aastha Kapoor, Amlan Barai, Bhushan Thakur, Alakesh Das, Sejal R. Patwardhan, Melissa Monteiro, Snehal Gaikwad, Amirali B. Bukhari, Pankaj Mogha, Abhijit Majumder, Abhijit De, Pritha Ray, Shamik Sen The failure of chemotherapeutic drugs in treatment of various cancers is attributed to the acquisition of drug resistance. However, the migration mechanisms of drug-resistant cancer cells remain incompletely understood. Here we address this question from a biophysical perspective by mapping the ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 28, 2017 Category: Molecular Biology Source Type: research

Selective inhibition reveals cyclin-dependent kinase 2 as another kinase that phosphorylates the androgen receptor at serine 81
Publication date: February 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 2 Author(s): Radek Jorda, Zuzana Bučková, Eva Řezníčková, Jan Bouchal, Vladimír Kryštof Several studies have revealed that cyclin-dependent kinases (CDK) can mediate phosphorylation of steroid receptors at multiple sites, including serine 81 of the androgen receptor (AR). Phosphorylation of S81 is required for AR nuclear translocation, an association with chromatin and also regulates endogenous AR-regulated transcription in response to hormones. Up to date, S81-phos...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 27, 2017 Category: Molecular Biology Source Type: research

A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic
Publication date: Available online 15 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Joana R. Lérias, Madalena C. Pinto, Hugo M. Botelho, Nikhil T. Awatade, Margarida C. Quaresma, Iris A.L. Silva, Podchanart Wanitchakool, Rainer Schreiber, Rainer Pepperkok, Karl Kunzelmann, Margarida D. Amaral An attractive possibility to treat Cystic Fibrosis (CF), a severe condition caused by dysfunctional CFTR, an epithelial anion channel, is through the activation of alternative (non-CFTR) anion channels. Anoctamin 1 (ANO1) was demonstrated to be a Ca2+-activated chloride channel (...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 17, 2017 Category: Molecular Biology Source Type: research

The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis
Publication date: Available online 16 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Isotta Lorenzi, Silke Oeljeklaus, Abhishek Aich, Christin Ronsör, Sylvie Callegari, Jan Dudek, Bettina Warscheid, Sven Dennerlein, Peter Rehling The three mitochondrial-encoded proteins, COX1, COX2, and COX3, form the core of the cytochrome c oxidase. Upon synthesis, COX2 engages with COX20 in the inner mitochondrial membrane, a scaffold protein that recruits metallochaperones for copper delivery to the CuA-Site of COX2. Here we identified the human protein, TMEM177 as a constituent of t...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 17, 2017 Category: Molecular Biology Source Type: research

An easy-to-use FRET protein substrate to detect calpain cleavage in vitro and in vivo
Publication date: February 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 2 Author(s): Christian-Scott E. McCartney, James A. MacLeod, Peter A. Greer, Peter L. Davies Calpain-1 and -2 are Ca2+-activated intracellular cysteine proteases that regulate a wide range of cellular functions through the cleavage of their protein substrates. Unlike degradative proteases, calpains make limited, transformative cleavages, typically in accessible sequences linking discrete subdomains, to irreversibly alter substrate functions. The biological roles of calpain and their interplay with calci...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 15, 2017 Category: Molecular Biology Source Type: research

Label-free discrimination analysis of de-differentiated vascular smooth muscle cells, mesenchymal stem cells and their vascular and osteogenic progeny using vibrational spectroscopy
This study established whether label-free vibrational spectroscopy can discriminate de-differentiated ‘synthetic’ SMCs from undifferentiated stem cells and their myogenic and osteogenic progeny in vitro, compared with conventional immunocytochemical and genetic analyses. TGF-β1- and Jagged1-induced myogenic differentiation of CD44 + mesenchymal stem cells was confirmed in vitro by immunocytochemical analysis of specific SMC differentiation marker expression (α-actin, calponin and myosin heavy chain 11), an epigenetic histone mark (H3K4me2) at the myosin heavy chain 11 locus, promoter transactivation ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 14, 2017 Category: Molecular Biology Source Type: research

Distorted leukocyte migration, angiogenesis, wound repair and metastasis in Tspan8 and Tspan8/CD151 double knockout mice indicate complementary activities of Tspan8 and CD51
Publication date: Available online 11 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Kun Zhao, Ulrike Erb, Thilo Hackert, Margot Zöller, Shijing Yue (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 13, 2017 Category: Molecular Biology Source Type: research

Distinct hypoxic regulation of preadipocyte factor-1 (Pref-1) in preadipocytes and mature adipocytes
This study suggests that Pref-1 is an endocrine factor which is synergistically increased by obesity and age. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 13, 2017 Category: Molecular Biology Source Type: research

RBM4-SRSF3-MAP4K4 splicing cascade modulates the metastatic signature of colorectal cancer cell
Publication date: Available online 11 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Jung-Chun Lin, Yuan-Chii Lee, Tse-Hua Tan, Yu-Chih Liang, Huai-Chia Chuang, Yang C. Fann, Kory R. Johnson, Ying-Ju Lin Alternative splicing (AS) of pre-messenger (m)RNA is a pivotal mechanism in expanding proteomic diversity, which determines the functions of mammalian cells. By conducting transcriptome analyses to profile splicing events in human colorectal cancer (CRC) tissues compared to adjacent normal counterparts, we noted differential splicing profiles of serine/arginine-rich splicing ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 12, 2017 Category: Molecular Biology Source Type: research

The inhibition of sphingomyelin synthase 1 activity induces collecting duct cells to lose their epithelial phenotype
Publication date: Available online 8 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Yamila Romina Brandán, Edith del Valle Guaytima, Nicolás Octavio Favale, Lucila Gisele Pescio, Norma B. Sterin-Speziale, María Gabriela Márquez Epithelial tissue requires that cells attach to each other and to the extracellular matrix by the assembly of adherens junctions (AJ) and focal adhesions (FA) respectively. We have previously shown that, in renal papillary collecting duct (CD) cells, both AJ and FA are located in sphingomyelin (SM)-enriched plasma membrane m...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 8, 2017 Category: Molecular Biology Source Type: research

Inhibition of poly(ADP-ribose) polymerase-1 alters expression of mitochondria-related genes in PC12 cells: Relevance to mitochondrial homeostasis in neurodegenerative disorders
Publication date: Available online 8 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Grzegorz A. Czapski, Magdalena Cieślik, Przemysław L. Wencel, Sylwia Wójtowicz, Robert P. Strosznajder, Joanna B. Strosznajder Alzheimer's disease (AD) is characterized by the release of amyloid beta peptides (Aβ) in the form of monomers/oligomers which may lead to oxidative stress, mitochondria dysfunction, synaptic loss, neuroinflammation and, in consequence, to overactivation of poly(ADP-ribose) polymerase-1 (PARP-1). However, Aβ peptides are also released in the brain is...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 8, 2017 Category: Molecular Biology Source Type: research

A heart-enriched antisense long non-coding RNA regulates the balance between cardiac and skeletal muscle triadin
Conclusion The lncRNA TRDN-AS regulates the balance between cardiac and skeletal isoforms of triadin. This finding may have implications for the treatment of heart failure. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 8, 2017 Category: Molecular Biology Source Type: research

Mechanical stretch increases L-type calcium channel stability in cardiomyocytes through a polycystin-1/AKT-dependent mechanism
Publication date: Available online 7 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): A. Córdova-Casanova, I. Olmedo, J.A. Riquelme, G. Barrientos, G. Sánchez, T.G. Gillette, S. Lavandero, M. Chiong, P. Donoso, Z. Pedrozo The L-type calcium channel (LTCC) is an important determinant of cardiac contractility. Therefore, changes in LTCC activity or protein levels could be expected to affect cardiac function. Several studies describing LTCC regulation are available, but only a few examine LTCC protein stability. Polycystin-1 (PC1) is a mechanosensor that regulates he...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 7, 2017 Category: Molecular Biology Source Type: research

Ectopic overexpression of MCPIP1 impairs adipogenesis by modulating microRNAs
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Magdalena Losko, Agata Lichawska-Cieslar, Maria Kulecka, Agnieszka Paziewska, Izabela Rumienczyk, Michal Mikula, Jolanta Jura Adipogenesis is a process of preadipocyte differentiation that requires action of numerous factors. Monocyte chemoattractant protein-1-induced protein 1 (MCPIP1) possesses the N-terminus of the PilT protein (PilT N-terminus or PIN domain) that has RNase properties. This protein degrades transcripts coding for inflammation and differentiation – related proteins. ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2017 Category: Molecular Biology Source Type: research

Characterization of gamma-tubulin filaments in mammalian cells
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Lisa Lindström, Maria Alvarado-Kristensson Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the &gam...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2017 Category: Molecular Biology Source Type: research

β-Arrestin2 directly or through GRK2 inhibits PKCβII activation in a ubiquitination-dependent manner
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Xiaohan Zhang, Mei Zheng, Ningning Sun, Kyeong-Man Kim The GRK/β-arrestin and PKC/PKA mediate the homologous and heterologous regulation of G protein-coupled receptors (GPCRs), respectively. Interaction between the two pathways is one of the most important issues in understanding the regulation of GPCRs. The present study investigated the regulatory effect of GRK2 and β-arrestins on PKC activation. The roles of GRK2 and β-arrestins in the functional regulation of PKC were asse...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2017 Category: Molecular Biology Source Type: research

Autophagy activation is required for influenza A virus-induced apoptosis and replication
Publication date: Available online 3 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): B. Yeganeh, S. Ghavami, Md.N. Rahim, T. Klonisch, A.J. Halayko, K.M. Coombs Autophagy and apoptosis are two major interconnected host cell responses to viral infection, including influenza A virus (IAV). Thus, delineating these events could facilitate the development of better treatment options and provide an effective anti-viral strategy for controlling IAV infection. We used A549 cells and mouse embryonic fibroblasts (MEF) to study the role of virus-induced autophagy and apoptosis, the cross...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 4, 2017 Category: Molecular Biology Source Type: research

Surface chemistry induces mitochondria-mediated apoptosis of breast cancer cells via PTEN/PI3K/AKT signaling pathway
Publication date: Available online 17 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Jing Zhang, Li Li, Yueting Peng, Yu Chen, Xiaoying Lv, Shun Li, Xiang Qin, Hong Yang, Chunhui Wu, Yiyao Liu Tumor cell can be significantly influenced by various chemical groups of the extracellular matrix proteins. However, the underlying molecular mechanisms involved in the interaction between cancer cells and functional groups in the extracellular matrix remain unknown. Using chemically modified surfaces with biological functional groups (CH3, NH2, OH), it was found that hydrophobic surface...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

α-Actinin-4 confers radioresistance coupled invasiveness in breast cancer cells through AKT pathway
Publication date: Available online 18 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Sejal Desai, Amlan Barai, Amirali B. Bukhari, Abhijit De, Shamik Sen Acquired radioresistance accompanied with increased metastatic potential is a major hurdle in effective radiotherapy of breast cancers. However, the nature of their inter-dependence and the underlying mechanism remains largely intangible. By employing radioresistant (RR) cell lines, we herein demonstrate that MCF-7 RR cells display phenotypic and molecular alterations evocative of epithelial to mesenchymal transition (EMT) wi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research