Label-free discrimination analysis of de-differentiated vascular smooth muscle cells, mesenchymal stem cells and their vascular and osteogenic progeny using vibrational spectroscopy
This study established whether label-free vibrational spectroscopy can discriminate de-differentiated ‘synthetic’ SMCs from undifferentiated stem cells and their myogenic and osteogenic progeny in vitro, compared with conventional immunocytochemical and genetic analyses. TGF-β1- and Jagged1-induced myogenic differentiation of CD44 + mesenchymal stem cells was confirmed in vitro by immunocytochemical analysis of specific SMC differentiation marker expression (α-actin, calponin and myosin heavy chain 11), an epigenetic histone mark (H3K4me2) at the myosin heavy chain 11 locus, promoter transactivation ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 14, 2017 Category: Molecular Biology Source Type: research

Distorted leukocyte migration, angiogenesis, wound repair and metastasis in Tspan8 and Tspan8/CD151 double knockout mice indicate complementary activities of Tspan8 and CD51
Publication date: Available online 11 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Kun Zhao, Ulrike Erb, Thilo Hackert, Margot Zöller, Shijing Yue (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 13, 2017 Category: Molecular Biology Source Type: research

Distinct hypoxic regulation of preadipocyte factor-1 (Pref-1) in preadipocytes and mature adipocytes
This study suggests that Pref-1 is an endocrine factor which is synergistically increased by obesity and age. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 13, 2017 Category: Molecular Biology Source Type: research

RBM4-SRSF3-MAP4K4 splicing cascade modulates the metastatic signature of colorectal cancer cell
Publication date: Available online 11 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Jung-Chun Lin, Yuan-Chii Lee, Tse-Hua Tan, Yu-Chih Liang, Huai-Chia Chuang, Yang C. Fann, Kory R. Johnson, Ying-Ju Lin Alternative splicing (AS) of pre-messenger (m)RNA is a pivotal mechanism in expanding proteomic diversity, which determines the functions of mammalian cells. By conducting transcriptome analyses to profile splicing events in human colorectal cancer (CRC) tissues compared to adjacent normal counterparts, we noted differential splicing profiles of serine/arginine-rich splicing ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 12, 2017 Category: Molecular Biology Source Type: research

The inhibition of sphingomyelin synthase 1 activity induces collecting duct cells to lose their epithelial phenotype
Publication date: Available online 8 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Yamila Romina Brandán, Edith del Valle Guaytima, Nicolás Octavio Favale, Lucila Gisele Pescio, Norma B. Sterin-Speziale, María Gabriela Márquez Epithelial tissue requires that cells attach to each other and to the extracellular matrix by the assembly of adherens junctions (AJ) and focal adhesions (FA) respectively. We have previously shown that, in renal papillary collecting duct (CD) cells, both AJ and FA are located in sphingomyelin (SM)-enriched plasma membrane m...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 8, 2017 Category: Molecular Biology Source Type: research

Inhibition of poly(ADP-ribose) polymerase-1 alters expression of mitochondria-related genes in PC12 cells: Relevance to mitochondrial homeostasis in neurodegenerative disorders
Publication date: Available online 8 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Grzegorz A. Czapski, Magdalena Cieślik, Przemysław L. Wencel, Sylwia Wójtowicz, Robert P. Strosznajder, Joanna B. Strosznajder Alzheimer's disease (AD) is characterized by the release of amyloid beta peptides (Aβ) in the form of monomers/oligomers which may lead to oxidative stress, mitochondria dysfunction, synaptic loss, neuroinflammation and, in consequence, to overactivation of poly(ADP-ribose) polymerase-1 (PARP-1). However, Aβ peptides are also released in the brain is...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 8, 2017 Category: Molecular Biology Source Type: research

A heart-enriched antisense long non-coding RNA regulates the balance between cardiac and skeletal muscle triadin
Conclusion The lncRNA TRDN-AS regulates the balance between cardiac and skeletal isoforms of triadin. This finding may have implications for the treatment of heart failure. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 8, 2017 Category: Molecular Biology Source Type: research

Mechanical stretch increases L-type calcium channel stability in cardiomyocytes through a polycystin-1/AKT-dependent mechanism
Publication date: Available online 7 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): A. Córdova-Casanova, I. Olmedo, J.A. Riquelme, G. Barrientos, G. Sánchez, T.G. Gillette, S. Lavandero, M. Chiong, P. Donoso, Z. Pedrozo The L-type calcium channel (LTCC) is an important determinant of cardiac contractility. Therefore, changes in LTCC activity or protein levels could be expected to affect cardiac function. Several studies describing LTCC regulation are available, but only a few examine LTCC protein stability. Polycystin-1 (PC1) is a mechanosensor that regulates he...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 7, 2017 Category: Molecular Biology Source Type: research

Ectopic overexpression of MCPIP1 impairs adipogenesis by modulating microRNAs
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Magdalena Losko, Agata Lichawska-Cieslar, Maria Kulecka, Agnieszka Paziewska, Izabela Rumienczyk, Michal Mikula, Jolanta Jura Adipogenesis is a process of preadipocyte differentiation that requires action of numerous factors. Monocyte chemoattractant protein-1-induced protein 1 (MCPIP1) possesses the N-terminus of the PilT protein (PilT N-terminus or PIN domain) that has RNase properties. This protein degrades transcripts coding for inflammation and differentiation – related proteins. ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2017 Category: Molecular Biology Source Type: research

Characterization of gamma-tubulin filaments in mammalian cells
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Lisa Lindström, Maria Alvarado-Kristensson Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the &gam...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2017 Category: Molecular Biology Source Type: research

β-Arrestin2 directly or through GRK2 inhibits PKCβII activation in a ubiquitination-dependent manner
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Xiaohan Zhang, Mei Zheng, Ningning Sun, Kyeong-Man Kim The GRK/β-arrestin and PKC/PKA mediate the homologous and heterologous regulation of G protein-coupled receptors (GPCRs), respectively. Interaction between the two pathways is one of the most important issues in understanding the regulation of GPCRs. The present study investigated the regulatory effect of GRK2 and β-arrestins on PKC activation. The roles of GRK2 and β-arrestins in the functional regulation of PKC were asse...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 6, 2017 Category: Molecular Biology Source Type: research

Autophagy activation is required for influenza A virus-induced apoptosis and replication
Publication date: Available online 3 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): B. Yeganeh, S. Ghavami, Md.N. Rahim, T. Klonisch, A.J. Halayko, K.M. Coombs Autophagy and apoptosis are two major interconnected host cell responses to viral infection, including influenza A virus (IAV). Thus, delineating these events could facilitate the development of better treatment options and provide an effective anti-viral strategy for controlling IAV infection. We used A549 cells and mouse embryonic fibroblasts (MEF) to study the role of virus-induced autophagy and apoptosis, the cross...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 4, 2017 Category: Molecular Biology Source Type: research

Surface chemistry induces mitochondria-mediated apoptosis of breast cancer cells via PTEN/PI3K/AKT signaling pathway
Publication date: Available online 17 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Jing Zhang, Li Li, Yueting Peng, Yu Chen, Xiaoying Lv, Shun Li, Xiang Qin, Hong Yang, Chunhui Wu, Yiyao Liu Tumor cell can be significantly influenced by various chemical groups of the extracellular matrix proteins. However, the underlying molecular mechanisms involved in the interaction between cancer cells and functional groups in the extracellular matrix remain unknown. Using chemically modified surfaces with biological functional groups (CH3, NH2, OH), it was found that hydrophobic surface...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

α-Actinin-4 confers radioresistance coupled invasiveness in breast cancer cells through AKT pathway
Publication date: Available online 18 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Sejal Desai, Amlan Barai, Amirali B. Bukhari, Abhijit De, Shamik Sen Acquired radioresistance accompanied with increased metastatic potential is a major hurdle in effective radiotherapy of breast cancers. However, the nature of their inter-dependence and the underlying mechanism remains largely intangible. By employing radioresistant (RR) cell lines, we herein demonstrate that MCF-7 RR cells display phenotypic and molecular alterations evocative of epithelial to mesenchymal transition (EMT) wi...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications
Publication date: Available online 31 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): M. Cecília Almadanim, Nuno M. Gonçalves, Margarida T.G. Rosa, Bruno M. Alexandre, André M. Cordeiro, Mafalda Rodrigues, Nelson J.M. Saibo, Cláudio M. Soares, Célia V. Romão, M. Margarida Oliveira, Isabel A. Abreu Plant calcium-dependent protein kinases (CDPKs) are key proteins implicated in calcium-mediated signaling pathways of a wide range of biological events in the organism. The action of each particular CDPK is strictly regulated by many mechanism...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Involvement of PpiD in sec-dependent protein translocation
Publication date: Available online 31 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Michaela Fürst, Yufan Zhou, Jana Merfort, Matthias Müller The periplasmic space in between the inner and outer membrane of Gram-negative bacteria contains numerous chaperones that are involved in the biogenesis and rescue of extra-cytosolic proteins. In contrast to most of those periplasmic chaperones, PpiD is anchored by an N-terminal transmembrane domain within the inner membrane of Escherichia coli. There it is located in close proximity to the SecY subunit of the SecYEG transloco...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Smurf2, an E3 ubiquitin ligase, interacts with PDE4B and attenuates liver fibrosis through miR-132 mediated CTGF inhibition
Publication date: Available online 31 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Yu Cai, Guanqun Huang, Lijie Ma, Ling Dong, She Chen, Xizhong Shen, Shuncai Zhang, Ruyi Xue, Deqiang Sun, Si Zhang We previously reported that Smad ubiquitin regulatory factor 2 (Smurf2) activity was decreased in human fibrotic livers. Here, we overexpressed Smurf2 in livers of transgenic mice and observed inhibited collagen deposition and hepatic stellate cell activation in fibrotic model induced by carbon tetrachloride treatment or bile duct ligation. Hepatic Smurf2 overexpression also inhib...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Disruption of adherens junction and alterations in YAP-related proliferation behavior as part of the underlying cell transformation process of alcohol-induced oral carcinogenesis
Publication date: Available online 2 November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Ayman Husari, Diana Hülter-Hassler, Thorsten Steinberg, Simon Daniel Schulz, Pascal Tomakidi Accumulating evidences indicate that alcohol might play a causative in oral cancer. Unfortunately, in vitro cell systems, uncovering the molecular background of the underlying cell transformation process, are rare. Therefore, this study was conducted, to identify molecular changes and characterize their putative cell behavioral consequences in epitheloid (EPI) and fibroblastoid (FIB) oral keratino...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

The Cks1/Cks2 axis fine-tunes Mll1 expression and is crucial for MLL-rearranged leukaemia cell viability
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): William Grey, Adam Ivey, Thomas A. Milne, Torsten Haferlach, David Grimwade, Frank Uhlmann, Edwige Voisset, Veronica Yu The Cdc28 protein kinase subunits, Cks1 and Cks2, play dual roles in Cdk-substrate specificity and Cdk-independent protein degradation, in concert with the E3 ubiquitin ligase complexes SCFSkp2 and APCCdc20. Notable targets controlled by Cks include p27 and Cyclin A. Here, we demonstrate that Cks1 and Cks2 proteins interact with both the MllN and MllC subunits of Mll1 (Mixe...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Human melanocortin 1 receptor-mediated ubiquitination of nonvisual arrestins. Role of Mahogunin Ring Finger 1 E3 ligase
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Marta Abrisqueta, Concepción Olivares, Cecilia Herraiz, María Castejón-Griñán, Julia Sirés-Campos, José C. García-Borrón, Celia Jiménez-Cervantes Signaling from the melanocortin 1 receptor (MC1R), a Gs protein-coupled receptor (GPCR) crucial for melanocyte proliferation and differentiation, is regulated by cytosolic β-arrestins (ARRBs). MC1R signaling is also negatively modulated by the E3-ubiquitin ligase Mahogunin R...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Translationally controlled tumor protein (TCTP) is required for TGF- β1 induced epithelial to mesenchymal transition and influences cytoskeletal reorganization
Publication date: January 2018 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1865, Issue 1 Author(s): Deepak Kumar Mishra, Pratibha Srivastava, Amod Sharma, Ramraj Prasad, Soubhagya Kumar Bhuyan, Rahuldev Malage, Pramod Kumar, Pramod Kumar Yadava Epithelial-mesenchymal transition (EMT) is a programed course of developmental changes resulting in the acquisition of invasiveness and mobility in cells. In cancer, this course is used by epithelial cells to attain movability. Translationally controlled tumor protein (TCTP) has been extensively characterized following the observation on tumor rever...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Nerve/glial antigen (NG) 2 is a crucial regulator of intercellular adhesion molecule (ICAM)-1 expression
In this study, we demonstrate that in pericytes and glioblastoma cells intercellular adhesion molecule (ICAM)-1, an essential protein for leukocyte adhesion and transmigration, underlies a NG2-dependent expression. As shown by flow cytometry, Western blot analysis and quantitative real-time polymerase chain reaction (qRT-PCR), silencing of NG2 in human placenta-derived pericytes increased the expression of ICAM-1. Pathway analyses revealed that this is mediated by extracellular-regulated-kinases (ERK) 1/2 signaling. Moreover, leukocyte adhesion to NG2 siRNA-treated pericytes was significantly enhanced when compared to scra...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Peptidyl-prolyl isomerase 1 (Pin1) preserves the phosphorylation state of tissue factor and prolongs its release within microvesicles
In conclusion, Pin1 is a fast-acting enzyme which may be utilised by cells to protect the phosphorylation state of TF in activated cells prolonging TF activity and release, and therefore ensuring adequate haemostasis. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research

Cathepsin D non-proteolytically induces proliferation and migration in human omental microvascular endothelial cell via activation of the ERK1/2 and PI3K/AKT pathways
We report, for the first time, that CathD significantly increases HOMEC proliferation and migration via a non-proteolytic mechanism resulting in activation of ERK1/2 and AKT. These data suggest that EOC cancer secreted CathD acts as an extracellular ligand and may play an important pro-angiogenic, and thus pro-metastatic, role by activating the omental microvasculature during EOC metastasis to the omentum. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 10, 2017 Category: Molecular Biology Source Type: research

Grb2 regulates the proliferation of hematopoietic stem and progenitors cells
Publication date: December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1864, Issue 12 Author(s): Catherine Frelin, Yishai Ofran, Julie Ruston, Michal Hayun, Yael Derdikman, Yasmine Khier, Kinneret Rozales, Benjamin Brenner, Norman Iscove, Tony Pawson, Igal Louria-Hayon Although Hematopoietic Stem and Progenitor Cell (HSPC) proliferation, survival and expansion have been shown to be supported by the cooperative action of different cytokines, little is known about the intracellular signaling pathways that are activated by cytokines upon binding to their receptors. Our study showed that ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 9, 2017 Category: Molecular Biology Source Type: research

Kinase-independent role of nuclear RIPK1 in regulating parthanatos through physical interaction with PARP1 upon oxidative stress
Publication date: Available online 7 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Ki-Hong Jang, Taeik Jang, Eunji Son, Soonjin Choi, Eunhee Kim Regulated necrosis occurs in various pathophysiological conditions under oxidative stress. Here, we report that receptor-interacting protein kinase 1 (RIPK1), a key player in one type of regulated necrosis (necroptosis), also participates in another type of poly (ADP-ribose) polymerase 1 (PARP1)-dependent regulated necrosis (parthanatos). Various biological signatures of parthanatos were significantly attenuated in Ripk1−/&minu...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 7, 2017 Category: Molecular Biology Source Type: research

Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein β degradation by adenosine receptor A1
In this study, we aimed to clarify the molecular mechanisms of the anti-adipogenic effect of theobromine in vitro and in vivo. ICR mice (4week-old) were administered with theobromine (0.1g/kg) for 7days. Theobromine administration attenuated gains in body and epididymal adipose tissue weights in mice and suppressed expression of adipogenic-associated genes in mouse adipose tissue. In 3T3-L1 preadipocytes, theobromine caused degradation of C/EBPβ protein by the ubiquitin-proteasome pathway. Pull down assay showed that theobromine selectively interacts with adenosine receptor A1 (AR1), and AR1 knockdown inhibited theobr...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 6, 2017 Category: Molecular Biology Source Type: research

The uL10 protein, a component of the ribosomal P-stalk, is released from the ribosome in nucleolar stress
Publication date: Available online 3 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Kamil Deryło, Barbara Michalec-Wawiórka, Dawid Krokowski, Leszek Wawiórka, Maria Hatzoglou, Marek Tchórzewski The ribosomal uL10 protein, formerly known as P0, is an essential element of the ribosomal GTPase-associated center responsible for the interplay with translational factors during various stages of protein synthesis. In eukaryotic cells, uL10 binds two P1/P2 protein heterodimers to form a pentameric P-stalk, described as uL10-(P1-P2)2, which represents the function...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2017 Category: Molecular Biology Source Type: research

Two mutations in mitochondrial ATP6 gene of ATP synthase, related to human cancer, affect ROS, calcium homeostasis and mitochondrial permeability transition in yeast
Publication date: Available online 3 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Katarzyna Niedzwiecka, Renata Tisi, Sara Penna, Malgorzata Lichocka, Danuta Plochocka, Roza Kucharczyk The relevance of mitochondrial DNA (mtDNA) mutations in cancer process is still unknown. Since the mutagenesis of mitochondrial genome in mammals is not possible yet, we have exploited budding yeast S. cerevisiae as a model to study the effects of tumor-associated mutations in the mitochondrial MTATP6 gene, encoding subunit 6 of ATP synthase, on the energy metabolism. We previously reported th...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2017 Category: Molecular Biology Source Type: research

AUF1 modulates TGF- β signal in renal tubular epithelial cells via post-transcriptional regulation of Nedd4L expression
Publication date: Available online 3 October 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Jing Yan, Feng Du, Sheng-Dong Li, Ye Yuan, Jing-Yi Jiang, Si Li, Xin-Yu Li, Zhen-Xian Du Posttranscriptional regulation process plays important roles in renal disease pathogenesis. AU-rich element RNA-binding protein (AUF1) interacts with and destabilizes mRNAs containing AU-rich elements (AREs) in their 3′UTR. The current study demonstrated that AUF1 was increased in unilateral ureteral obstruction (UUO) animal models. While proliferation and migration of HK2 cells was unaltered by AUF1 ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2017 Category: Molecular Biology Source Type: research

Hepatitis B core protein promotes liver cancer metastasis through miR-382-5p/DLC-1 axis
In conclusion, our findings revealed that HBc promoted HCC motility by regulating the miR-382-5p/DLC-1 axis, which might provide a novel target for clinical diagnosis and treatment. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2017 Category: Molecular Biology Source Type: research

Cold-sensing TRPM8 channel participates in circadian control of the brown adipose tissue
Publication date: December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1864, Issue 12 Author(s): Maria Nathália Moraes, Leonardo Vinicius Monteiro de Assis, Felipe dos Santos Henriques, Miguel Luiz Batista Jr, Ali D. Güler, Ana Maria de Lauro Castrucci Transient receptor potential (TRP) channels are known to regulate energy metabolism, and TRPM8 has become an interesting player in this context. Here we demonstrate the role of the cold sensor TRPM8 in the regulation of clock gene and clock controlled genes in brown adipose tissue (BAT). We investigated TrpM8 temporal profil...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2017 Category: Molecular Biology Source Type: research

Testosterone/bicalutamide antagonism at the predicted extracellular androgen binding site of ZIP9
Publication date: December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1864, Issue 12 Author(s): Ahmed Bulldan, Viveka Nand Malviya, Neha Upmanyu, Lutz Konrad, Georgios Scheiner-Bobis ZIP9 is a Zn2+ transporter, testosterone receptor, and mediator of signaling events through G-proteins. Despite these pivotal properties, however, its physiological and pathophysiological significance has not yet been comprehensively addressed. Using a cell line that lacks the classical androgen receptor we show that ZIP9-mediated phosphorylation of Erk1/2, CREB, or ATF-1 and expression of claudin-5 and ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2017 Category: Molecular Biology Source Type: research

Cell micropatterning reveals the modulatory effect of cell shape on proliferation through intracellular calcium transients
Publication date: December 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1864, Issue 12 Author(s): Jie Tong, Ying Qi, Xiangmiao Wang, Liyin Yu, Chang Su, Wenjun Xie, Jianbao Zhang The mechanism by which cell shape regulates the function of the cell is one of the most important biological issues, but it remains unclear. Here, we investigated the effect of the regulation of cell shape on proliferation by using a micropatterning approach to confine MC3T3-E1 cells into specific shapes. Our results show that the proliferation rate for rectangle-, triangle-, square- and circle-shaped osteobla...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 4, 2017 Category: Molecular Biology Source Type: research

AKAP-Lbc mediates protection against doxorubicin-induced cardiomyocyte toxicity
In conclusion, AKAP-Lbc emerges as a coordinator of signals that protect cardiomyocytes against the toxic effects of DOX. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 2, 2017 Category: Molecular Biology Source Type: research

Regions of MRAP2 required for the inhibition of orexin and prokineticin receptor signaling
In this study we demonstrate a new role of MRAP2 in the regulation of the orexin receptor 1 (OX1R) and identify the specific regions of MRAP2 required for the regulation of OX1R and PKR1. Importantly, like MC4R and PKRs, OX1R is predominately expressed in the brain where it regulates food intake. By demonstrating that MRAP2 modulates the activity of OX1R we further establish the critical role of MRAP2 in the control of energy homeostasis. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - October 2, 2017 Category: Molecular Biology Source Type: research

New intracellular activities of matrix metalloproteinases shine in the moonlight
This article is part of a Special Issue entitled: Matrix Metalloproteinases edited by Rafael Fridman. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 19, 2017 Category: Molecular Biology Source Type: research

Matrix metalloproteinases as regulators of inflammatory processes
This article is part of a Special Issue entitled: Matrix Metalloproteinases edited by Rafael Fridman. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 19, 2017 Category: Molecular Biology Source Type: research

The role of matrix metalloproteinases in aging: Tissue remodeling and beyond
Publication date: November 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1864, Issue 11, Part A Author(s): Sandra Freitas-Rodríguez, Alicia R. Folgueras, Carlos López-Otín Proteases are a set of enzymes that have been involved in multiple biological processes throughout evolution. Among them, extracellular matrix (ECM) remodeling has emerged as one of the most relevant functions exerted by these proteins, being essential in the regulation of critical events such as embryonic development or tissue homeostasis. Hence, it is not surprising that dysregulation in any pro...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 19, 2017 Category: Molecular Biology Source Type: research

Metalloproteinases in extracellular vesicles
This article is part of a Special Issue entitled: Matrix Metalloproteinases edited by Rafael Fridman. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 19, 2017 Category: Molecular Biology Source Type: research

Next generation matrix metalloproteinase inhibitors — Novel strategies bring new prospects
This article is part of a Special Issue entitled: Matrix Metalloproteinases edited by Rafael Fridman. (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 19, 2017 Category: Molecular Biology Source Type: research

Suspension state increases reattachment of breast cancer cells by up-regulating lamin A/C
In conclusion, it was demonstrated that suspension state promoted the reattachment of breast tumor cells by up-regulating lamin A/C via cytoskeleton disruption. These findings highlight the important role of suspension state for tumor cells in tumor metastasis. Graphical abstract (Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research)
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 15, 2017 Category: Molecular Biology Source Type: research

Transcription factor CUX1 is required for intestinal epithelial wound healing and targets the VAV2-RAC1 Signalling complex
Publication date: Available online 8 September 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Roxanne Latreille, Raphaëlle Servant, Mathieu Darsigny, Sébastien Marcoux, Christine Jones, Nathalie Perreault, François Boudreau Intestinal epithelial cells form a protective barrier in limiting gut luminal content potentially harmful to the host. Upon gut epithelium injury, several signals instruct epithelial cells to undergo a rapid healing process. Defects in this process induce inflammatory responses and can further evolve into chronic gut inflammatory diseases. We pre...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 9, 2017 Category: Molecular Biology Source Type: research

The ILK-MMP9-MRTF axis is crucial for EndMT differentiation of endothelial cells in a tumor microenvironment
Publication date: Available online 8 September 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Wojciech M. Ciszewski, Katarzyna Sobierajska, Marta E. Wawro, Wanda Klopocka, Natasza Chefczyńska, Angelika Muzyczuk, Kamil Siekacz, Agata Wujkowska, Jolanta Niewiarowska Increasing evidence indicates that the tumor microenvironment is a critical factor supporting cancer progression, chemoresistance and metastasis. Recently, cancer-associated fibroblasts (CAFs) have been recognized as a crucial tumor stromal component promoting cancer growth and invasiveness via modulation of the extracellul...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 9, 2017 Category: Molecular Biology Source Type: research

Chemicals or mutations that target mitochondrial translation can rescue the respiratory deficiency of yeast bcs1 mutants
Publication date: Available online 6 September 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): C. Panozzo, A. Laleve, D. Tribouillard-Tanvier, J. Ostojić, C. Sellem, G. Friocourt, A. Bourand-Plantefol, A. Burg, A. Delahodde, M. Blondel, G. Dujardin Bcs1p is a chaperone that is required for the incorporation of the Rieske subunit within complex III of the mitochondrial respiratory chain. Mutations in the human gene BCS1L (BCS1-like) are the most frequent nuclear mutations resulting in complex III-related pathologies. In yeast, the mimicking of some pathogenic mutations causes a respira...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 7, 2017 Category: Molecular Biology Source Type: research

Noradrenaline, oxymetazoline and phorbol myristate acetate induce distinct functional actions and phosphorylation patterns of α1A-adrenergic receptors
Publication date: Available online 6 September 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Rocío Alcántara-Hernández, Aurelio Hernández-Méndez, M. Teresa Romero-Ávila, Marco A. Alfonzo-Méndez, André S. Pupo, J. Adolfo García-Sáinz In LNCaP cells that stably express α1A-adrenergic receptors, oxymetazoline increased intracellular calcium and receptor phosphorylation, however, this agonist was a weak partial agonist, as compared to noradrenaline, for calcium signaling. Interestingly, oxymetazoline-induced rece...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 7, 2017 Category: Molecular Biology Source Type: research

LRRK2 functions as a scaffolding kinase of ASK1-mediated neuronal cell death
In this study, we showed that LRRK2 played an important role in neuronal cell death by directly phosphorylating and activating apoptosis signal-regulating kinase 1 (ASK1). LRRK2 phosphorylated ASK1 at Thr832 that is adjacent to Thr845, which serves as an autophosphorylation site. Moreover, results of binding and kinase assays showed that LRRK2 acted as a scaffolding protein by interacting with each components of the ASK1–MKK3/6–p38 MAPK pathway through its specific domains and increasing the proximity to downstream targets. Furthermore, LRRK2-induced apoptosis was suppressed by ASK1 inhibition in neuronal stem ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 7, 2017 Category: Molecular Biology Source Type: research

Quantification of volume and lipid filling of intracellular vesicles carrying the ABCA3 transporter
Publication date: Available online 6 September 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Stefanie Höppner, Susanna Kinting, Adriano A. Torrano, Ulrike Schindlbeck, Christoph Bräuchle, Ralf Zarbock, Thomas Wittman, Matthias Griese The ABCA3 lipid transporter is located in the limiting membrane of lamellar bodies (LBs) in type-II-pneumocytes. Mutations within the ABCA3 gene may functionally impair the transporter, causing lung diseases in newborns, children and adults. Assays to quantify volume and lipid filling of the LBs on the level of the vesicular structures and ther...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 6, 2017 Category: Molecular Biology Source Type: research

How to control an intracellular proteolytic system: Coordinated regulatory switches in the mycobacterial pup-proteasome system
Publication date: Available online 6 September 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Eyal Gur, Maayan Korman, Nir Hecht, Ofir Regev, Shai Schlussel, Nimrod Silberberg, Yifat Elharar Intracellular proteolysis is critical for the proper functioning of all cells, owing to its involvement in a wide range of processes. Because of the destructive nature of protein degradation, intracellular proteolysis is restricted by control mechanisms at almost every step of the proteolytic process. Understanding the coordination of such mechanisms is a challenging task, especially in systems as...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 6, 2017 Category: Molecular Biology Source Type: research

ErbB3 Interacts with Hrs and is sorted to lysosomes for degradation
We report that ErbB3 is internalized by clathrin-mediated endocytosis both in the presence and absence of heregulin. Moreover, we show that both proteasomal and lysosomal activity regulate ErbB3 degradation. Although steady-state expression of ErbB3 is regulated by proteasomal activity to a large extent, probably linked to a previously identified ER-localized quantity control, the results indicate that internalization, both constitutive and ligand-induced, causes lysosomal degradation of ErbB3. Furthermore, we show that ErbB3 interacts with the ESCRT-0 subunit Hrs both in the presence and absence of heregulin. This indicat...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - September 2, 2017 Category: Molecular Biology Source Type: research