Phytoconstituents from the leaves of Dracaena cochinchinensis (Lour.) S. C. Chen
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Lin Hu, Fang-Fang Wang, Xing-Hong Wang, Qing-Song Yang, Yong Xiong, Wen-Xing Liu Five flavonoids, four feruloyl amide derivatives, pinoresinol, lanost-9-en-3β-ol and three steroids from the leaves of Dracaena cochinchinensis (Lour.) S. C. Chen. Of these, compound 4 was identified as a new homoisoflavanone. This publication is the first reported purification of compounds 3, 4, 6–12 from D. cochinchinensis. We also indicate the chemotaxonomic importance of these metabolites. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - September 27, 2015 Category: Biochemistry Source Type: research

Genetic diversity of cultured populations of giant freshwater prawn (Macrobrachium rosenbergii) in China using mtDNA COI and 16S rDNA markers
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Hai Nguyen Thanh, Qigen Liu, Liangjie Zhao, Hong Zhang, Jun Liu, Dang Nguyen Hai Partial sequences of mitochondrial DNA 16S rDNA and COI genes (395 bp and 498 bp respectively) were sequenced from samples of ten cultured populations of Macrobrachium rosenbergii (Giant Freshwater Prawn – GFP) in Zhejiang, Guangdong and Guangxi Provinces in China and two wild populations of GFP from Mekong River and Dongnai River in Viet Nam. Five haplotypes of 16S rDNA were identified in the 360 samples. The wild population...
Source: Biochemical Systematics and Ecology - September 25, 2015 Category: Biochemistry Source Type: research

MSAP-based analysis of DNA methylation diversity in tobacco exposed to different environments and at different development phases
In this study, we employed methylation-sensitive amplification polymorphism (MSAP) technology to investigate the extent and patterns of methylation in tobacco planted in five different areas of Dali County, Yunnan Province. All of the samples were collected during the vegetative and reproductive growth phases. The data obtained from the MSAP analysis showed that full-methylation and non-methylation modifications were predominant and that the hemi-methylation modification ratio was markedly lower. The PCA and UPGMA analysis results indicated that the samples collected from different areas during the vegetative and reproduct...
Source: Biochemical Systematics and Ecology - September 23, 2015 Category: Biochemistry Source Type: research

The complete mitochondrial genome of Eriocheir japonica sinensis (Decapoda: Varunidae) and its phylogenetic analysis
In this study, the complete mitogenome of Eriocheir japonica sinensis (Decapoda: Varunidae) was determined to be 16,378 bp, including 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and a D-loop region. The AT skew of the E. j. sinensis mitogenome was slightly negative (−0.016), indicating a higher number of T compared with A nucleotides. The nucleotide composition of the mitogenome was also biased toward A + T nucleotides (71.6%). All PCGs were initiated by ATN codons. Eight of the 13 PCGs harbored the incomplete termination codon by T, or TA. All other tRNA genes displayed a typical clo...
Source: Biochemical Systematics and Ecology - September 21, 2015 Category: Biochemistry Source Type: research

Chemical constituents from the tubers of Pinellia ternata (Araceae) and their chemotaxonomic interest
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Ying-Ying Wu, Xiao-Xiao Huang, Meng-Yu Zhang, Le Zhou, Dan-Qi Li, Zhuo-Yang Cheng, Ling-Zhi Li, Ying Peng, Shao-jiang Song This work describes the isolation and characterization of twenty compounds from the tubers of Pinellia ternata (Thunb.) Breit, including 17 lignanoids (1–17) and three alkaloids (18–20). All structures were established based on analysis of their spectroscopic data. It is the first report of compounds 3–11 and 13–18 from the genus Pinellia, and the first report of compounds ...
Source: Biochemical Systematics and Ecology - September 19, 2015 Category: Biochemistry Source Type: research

Evaluation of the geographical pattern of genetic diversity of Glycine soja and Glycine max based on four single copy nuclear gene loci: For conservation of soybean germplasm
This study suggests that native populations of G. soja from different geo-ecological regions should be protected to conserve the genetic diversity. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - September 19, 2015 Category: Biochemistry Source Type: research

Initial diversification, glacial survival, and continuous range expansion of Gentiana straminea (Gentianaceae) in the Qinghai–Tibet Plateau
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Zhiqiang Lu, Ping Chen, Xiaotao Bai, Jianmei Xu, Xiaodong He, Zhimin Niu, Dongshi Wan The Qinghai–Tibet Plateau (QTP) has been considered as one of the most sensitive regions to climate change on Earth, and the growth and distribution of alpine species on this plateau have been suggested to depend greatly on their ability to survive within a small range of temperatures. However, the responses of most species in the QTP to the Quaternary climatic oscillation remain largely unknown. We sequenced two cpDNA fragments ...
Source: Biochemical Systematics and Ecology - September 19, 2015 Category: Biochemistry Source Type: research

Inter-regional variation on leaf surface defenses in native and non-native Centaurea solstitialis plants
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Gastón J. Sotes, Lohengrin A. Cavieres, Daniel Montesinos, António Xavier Pereira Coutinho, Walter José Peláez, Susana M.M. Lopes, Teresa M.V.D. Pinho e Melo Plant chemical defenses can be qualitative (toxins) to face generalist herbivores and quantitative (digestibility reducers) to specialists. Trichomes can produce chemicals, but also acts as a quantitative defense and in water loss. The shifting defense hypothesis (SDH) poses that invasive plants reduce the production of qualitative defen...
Source: Biochemical Systematics and Ecology - September 18, 2015 Category: Biochemistry Source Type: research

Chemical constituents from Euphorbia hirta
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): En-Tao Li, Kuan-Hui Liu, Ming-Hui Zang, Xue-Lan Zhang, Hai-Qiang Jiang, Hong-Lei Zhou, De-Yun Wang, Jia-Guo Liu, Yuan-Liang Hu, Yi Wu Thirty-one compounds, including fourteen triterpenoids (1–14), seven coumarins (15–21), four lignans (22–25), and six diterpenes (26–31) were isolated from the aerial part of Euphorbia hirta. The structures were elucidated by spectroscopic methods and by comparison of their reported spectroscopic data. These compounds have shown the chemical relationships betwee...
Source: Biochemical Systematics and Ecology - September 18, 2015 Category: Biochemistry Source Type: research

Diversity in local cultivars of Pisum sativum collected from home gardens in Sweden
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Svein Øivind Solberg, Agnese Kolodinska Brantestam, Kerstin Olsson, Matti Wiking Leino, Jens Weibull, Flemming Yndgaard Although one would assume that finding any local cultivars in home gardens in a modern society such as Sweden is unlikely, such cultivars were in fact found. More than 170 seed accessions of vegetables, pulses and other seed-propagated garden crops maintained in home gardens and dating back at least to the 1950s have been assembled following the nationwide ‘Seed Call’. Of these, 32 ga...
Source: Biochemical Systematics and Ecology - September 18, 2015 Category: Biochemistry Source Type: research

Phylogenetic relationships among sections Vicia, Sepium and Lathyroides of Vicia subgenus Vicia: isozyme evidence
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Vello Jaaska Variation of 16 isozymes in V icia barbazitae and in two couples of morphologically similar vetch species, V icia grandiflora – Vicia qatmensis and Vicia lathyroides – V icia cuspidata, in comparison with seven other species of sections Vicia and Sepium is described in order to get new evidence about their differentiation, phylogenetic and sectional position. The cladistic parsimony analysis of the isozyme data using Vicia bithynica of section Pseudolathyrus as an outgroup placed V. barbazitae, V. qat...
Source: Biochemical Systematics and Ecology - September 13, 2015 Category: Biochemistry Source Type: research

COI gene sequence analysis for testing cyclical mating in securing genetic diversity of Macrobrachium rosenbergii
In this study we analyzed sequences of the COI gene to investigate the genetic structure and diversity in four populations of the Giant Malaysian Prawn, Macrobrachium rosenbergii, and to test the effectiveness of cyclical mating as means of securing genetic diversity. Forty-three haplotypes were generated for the parental populations; most of these were unique, with moderate genetic and low nucleotide diversities. However Tajima's neutrality test indicated a significant (p < 0.05) departure of the Negeri Sembilan population from the mutation–drift equilibrium, suggesting that it may be losing genetic...
Source: Biochemical Systematics and Ecology - September 9, 2015 Category: Biochemistry Source Type: research

A chemotaxonomic study of phytochemicals in Hedyotis corymbosa
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Hongquan Li, Chun Li, Bohou Xia, Yamin Zhou, Limei Lin, Duanfang Liao An investigation of Hedyotis corymbosa resulted in the isolation of eighteen compounds, including six iridoids (1–6), six monocyclic phenols (7–12), one triterpene (13), two sterols (14–15), one anthranquinone (16), one oxylipin (17) and one coumarin (18). The compounds were identified as scandoside methyl ester (1), deacetyl asperulosidic acid (2), deacetyl asperulosidic acid methyl ester (3), daphylloside (4), 10-O-benzoyl deacetyl ...
Source: Biochemical Systematics and Ecology - September 4, 2015 Category: Biochemistry Source Type: research

Localization of 45S and 5S rDNA sites and karyotype of Chrysanthemum and its related genera by fluorescent in situ hybridization
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Xiangyu Qi, Fei Zhang, Zhiyong Guan, Haibin Wang, Jiafu Jiang, Sumei Chen, Fadi Chen The phylogenetic relationships among Chrysanthemum and its related genera (Anthemideae, Asteraceae) is poorly understood. In the present study, these relationships were investigated using 45S and 5S ribosomal DNA (rDNA)-targeted fluorescent in situ hybridization. The results showed that there were two 45S rDNA signals present in Crossostephium chinense, four 45S rDNA signals in Cercidiphyllum japonicum, Artemisia sieversiana, Artemisia ...
Source: Biochemical Systematics and Ecology - August 30, 2015 Category: Biochemistry Source Type: research

Tetranucleotide microsatellites for the barnacle Megabalanus coccopoma (Darwin, 1854)
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): A.M. Reigel, J.S. Harrison, D.F. Gleason The barnacle Megabalanus coccopoma is indigenous to the tropical Pacific Ocean, but was recently introduced to the coastal waters of the southeastern U.S.A. As part of a larger effort to investigate the population dynamics of this introduction, we designed 13 microsatellite primers specific to M. coccopoma and developed the accompanying polymerase chain reaction (PCR) conditions. We tested these primers on 42 individuals of M. coccopoma collected from two sampling locations in coasta...
Source: Biochemical Systematics and Ecology - August 29, 2015 Category: Biochemistry Source Type: research

Chemical constituents of Chroogomphus rutilus (Schaeff.) O.K. Mill
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Jie Luo, Wei Zhou, Shuo Cao, Heming Zhu, Changhao Zhang, Mei Jin, Gao Li This work describes the isolation of thirty-two compounds from Chroogomphus rutilus, including eight sterols (1–8), five phenols (9–13), three nucleosides (14–16), two monosaccharide derivatives (17–18) and fourteen coumarins (19–32). The chemical structures of the compounds were elucidated on the basis of spectral data (1H and 13C NMR) and by comparisons of spectroscopic data with reported values in the literatures. Tw...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Assessing genetic diversity in Ziziphus jujuba ‘Jinsixiaozao’ using morphological and microsatellite (SSR) markers
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Zhendong Zhang, Jiao Gao, Decang Kong, Aihua Wang, Shizhe Tang, Yingyue Li, Xiaoming Pang Ziziphus jujuba ‘Jinsixiaozao’ is one of the most elite Chinese jujube variety with a long cultivation history. There are many different types and newly developed accessions of ‘Jinsixiaozao’ reported, however the names are in chaotic. For the accurate identification of the accessions and estimating the genetic diversity, the diversity and relationships of the 45 ‘Jinsixiaozao’ accessions were eva...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Molecular genetic diversity of curcuminoid genes in Curcuma amada: Curcuminoid variation, consideration on species boundary and polyploidy
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Syed Abdullah Gilani, Akira Kikuchi, Takayoshi Shimazaki, Noladhi Wicaksana, Wunna, Kazuo N. Watanabe Experiments were undertaken to test the hypothesis that in Curcuma amada the curcuminoid content is related to ploidy level and sequence variations in the genes related to curcuminoid biosynthesis. Eight curcumin-containing accessions of C. amada and six curcumin-free accessions were analyzed. Flow cytometry revealed that the ploidy level was higher in curcumin-containing accessions than in curcumin-free accessions (avera...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Assessment of genetic diversity among androdioecious ancient Osmanthus fragrans trees by SSR markers
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): YiFan Duan, Meng Li, Xian Gui Yi, HuaChen Wang, XianRong Wang, QiBai Xiang The well known ornamental plant, Osmanthus fragrans, is one of the rare androdioecious species in nature. Determining the genetic diversity of this species may assist in its conservation and genetic improvement. Data of 10 ancient tree populations of O. fragrans were used to assess its genetic diversity and population structure with microsatellite markers. A high level of genetic diversity was observed with POPGENE. As anticipated the heterozygosit...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Characterization of new microsatellite loci from the razor clam (Sinonovacula constricta) and transferability to related species
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Xueping Wu, Yanwei Feng, Haitao Ma, Ying Pan, Xiangquan Liu Simple sequence repeats (SSRs) have been widely used in marine animals, but were seldom used directly to test intra- and inter-specific transferability in the populations of related species. 19 new pairs of microsatellite primers were obtained from Sinonovacula constricta. The allele number of these markers ranged from 3 to 15. The observed and expected heterozygosities changed from 0.033 to 1.000 and from 0.033 to 0.912, respectively. Three loci (SC3-8, SC3-19, a...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Chemical constituents of the rhizomes and roots of Gentiana scabra (Gentianaceae)
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Wei Li, Wei Zhou, Sang Hee Shim, Young Ho KimGraphical abstract (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Molecular identification and allopatric divergence of the white pine species in China based on the cytoplasmic DNA variation
In this study, we examined these possibilities in a group of white pines in China. We sampled 449 individuals from 60 natural populations of seven species from sect. Quinquefoliae subsect. Strobus. We sequenced four chloroplast DNA regions (around 3100 bp in length) and two mitochondrial DNAs (around 1000 bp in length). We identified 21 chlorotypes and 10 mitotypes. Both chlorotypes and mitotypes recovered from four species with long disjunction and restricted distributions in northern or northwestern China, Pinus sibirica, Pinus koraiensis, Pinus wallichiana and Pinus pumila are species-specific, suggesting that...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Chemical constituents from leaves of Cinnamomum subavenium
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Xincai Hao, Jing Chen, Yongji Lai, Ming Sang, Guangmin Yao, Yongbo Xue, Zengwei Luo, Geng Zhang, Yonghui Zhang A new eudesmane sesquiterpene glycoside, 1α,6β-dihydroxy-5,10-bis-epi-eudesm-15-carboxaldehyde-6-O-β-d-Glucopyranoside (1), together with eleven known compounds (2–12) were isolated from the leaves of Cinnamomum subavenium Miq. Their structures were elucidated by a combination of spectroscopic data analysis and comparison with literature data. All compounds were isolated from C. subaveniu...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Highly efficient identification of thousands of microsatellite loci in the pearl oyster (Pinctada martensii) from RNA-Seq
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Yu-Song Guo, Xue-Ying Wang, Zhong-Duo Wang, Xiao-Xia Zhao, Qing-Heng Wang, Yue-Wen Deng, Xiao-Dong Du High-throughput RNA-Seq affords a cost and time effective means of obtaining large numbers of genetic markers for aquatic genomics. Here, we present thousands of novel microsatellite loci developed for the pearl oyster, Pinctada martensii from the Illumina HiSeq™ 2000 library of the pearl sac. Free user-friendly bioinformatics tools were employed to screen for microsatellite loci and design appropriate primers in 1...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Correlation analyses between molecular perspective and phytochemical variations in Coptis chinensis Franch
In this study, we determined 59 samples from 12 different habits. Other than the samples from Zhenping, the content of six selected alkaloids in C. chinensis did not differ significantly among the habits. Furthermore, the results of the genetic analysis showed that the genetic diversity and the genetic distance among the samples were low, suggesting that the C. chinensis samples from different habits had the same genetic characteristics. These results would suggest that the quality of the drugs are not influenced by the habitats the plant is growing in. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Chemical constituents from Cynanchum paniculatum (Bunge) Kitag
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Ya-Le Niu, Xi Chen, Yi Wu, Hai-Qiang Jiang, Xue-Lan Zhang, En-Tao Li, You-Ying Li, Hong-Lei Zhou, Jia-Guo Liu, De-Yun Wang Twenty-nine compounds, including five acetophenone derivatives (1–5), three phenanthroindolizidine alkaloids (12–14), seven pentacyclic triterpenoids (15–21) and five C21 steroidal sapogenins (22–26), were isolated from the root of Cynanchum paniculatum (Bunge) Kitag. Their structures were determined by spectroscopic methods and comparison with reported data. Moreover, the ...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Effect of allelopathic potential from selected aquatic macrophytes on algal interaction in the polluted water
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Shengpeng Zuo, Zhangshun Fang, Shanyun Yang, Kun Wan, Yajuan Han Pollutants and macrophyte allelopathy can drive the change of algal interactions in aquatic ecosystems. Eutrophication, Pb and Polychlorinated biphenyls could induce a positive interaction between two green algae or blue algae in the stationary and death phases, as well as a negative interaction between these algae in the lag and exponential phases. Similarly, the negative interaction between a green alga and a blue alga always occurred in whether polluted co...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Genetic structure of Pinus henryi and Pinus tabuliformis: Natural landscapes as significant barriers to gene flow among populations
In this study, we analyzed genetic differentiation and the geographic boundary between Pinus henryi and Pinus tabuliformis using chloroplast microsatellite markers. Our data show high levels of among-population differentiation, consistent with the effects of historical demographic bottlenecks, local adaptation and climate effects. Three main geographic boundaries coinciding with mountain systems indicate natural landscapes, such as large rivers, and habitat loss caused by anthropogenic deforestation, are significant barriers to genetic exchange among populations. The divergence between populations in the eastern and wester...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Isolation and characterization of 19 polymorphic microsatellite loci from Neosalanx taihuensis, a rapidly invasive and adaptative species
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Hong-Yan Liu, Cun-Yao Li, Fei Xiong Neosalanx taihuensis can very rapidly adapt to new environmental conditions and quickly becomes the dominant species when introduced to lakes and reservoirs. To better understand the genetic mechanism responsible for this remarkable ability to invade and adapt to diverse ecosystems, 19 polymorphic microsatellite loci were isolated from the genomic DNA of N. taihuensis. These microsatellite loci were identified from 24 individuals from an invasive population sampled in Fuxian Lake, Yunnan P...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Chemical constituents from Cremanthodium brunneo-pilosum S. W. Liu
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Zhi-Zhou He, Li-Sheng Ding, Rui-Hong Xu, Bo Wan, Shu-Lin Peng, Hao-Huai Liu, Yuan Zeng Fourteen compounds were isolated from Cremanthodium brunneo-pilosum, including two steroids (1 and 14), five sesquiterpenoids (2–6), six flavonoids (7–12) and one isoflavone (13). Graveolide (2) was obtained from a plant for the first time. 5,7-Dihydroxy-3,6,3′,4′-tetramethoxy-flavone (9), centaureidin (10) and tectoridin (13) have not been isolated from the family Compositae. The chemotaxonomic data support the...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

The chemical defenses of millipedes (diplopoda): Biochemistry, physiology and ecology
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): William A. Shear Millipedes are some of the earliest examples of terrestrial animals, and fossils from the early Carboniferous Period indicate they were also some of the earliest prey. These fossils record ozopores, the openings of chemical defense glands, occurring along the length of the body. The secretions of these glands may consist of topical irritants, repellents, antifeedants, or, in the case of the large and widespread Order Polydesmida, hydrogen cyanide (HCN) gas that can be fatal to other arthropods or even small ve...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Genetic diversity and structure of Pinus dabeshanensis revealed by expressed sequence tag-simple sequence repeat (EST-SSR) markers
Publication date: August 2015 Source:Biochemical Systematics and Ecology, Volume 61 Author(s): Xiao-yan Xiang, Zhong-xin Zhang, Ren-yan Duan, Xiao-ping Zhang, Gan-lin Wu Assessing patterns of genetic variation in rare endangered species is critical for developing both in situ and ex situ conservation strategies. Pinus dabeshanensis Cheng et Law is an endangered species endemic to the Dabieshan Mountains of eastern China. To obtain fundamental information of genetic diversity, population history, effective population size, and gene flow in this species, we explored patterns of genetic variation of natural populations...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Isolation and characterization of nine tetranucleoide microsatellite loci for the secretive limbless lizards of the genus Anniella (Anguidae)
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Guinevere O.U. Wogan, Julie Kapelke, Kevin A. Feldheim, Theodore J. Papenfuss, Rauri C.K. Bowie Limbless lizards of the genus Anniella are found in the western United States and Mexico. Until recently only two species were known, but four new species have since been described. Since these lizards are fossorial, not much is known about the nature of gene flow within species, or if gene flow occurs across species boundaries in regions of overlap. Since these lizards are of conservation interest, we isolated and developed ni...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Flavanone derivatives from Macaranga tanarius
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Yana M. Syah, Emilio L. Ghisalberti A new flavanone, epoxynymphaeol C (1), was isolated from the acetone extract of the leaves of Macaranga tanarius, together with two known compounds, nymphaeols B (2) and C (3). The structure of the new compound was determined by NMR and mass spectral data. Chemotaxonomic significance for the presence of the flavanone derivatives in M. tanarius is discussed. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Population genetic structure in wild and hatchery populations of white cloud mountain minnow (Tanichthys albonubes): Recommendations for conservation
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Jin-Zhen Luo, Hung-Du Lin, Fei Yang, Zu-Sheng Yi, Bosco-Puilok Chan, Jun Zhao We used mitochondrial (mt) cytochrome b gene (cyt b) to compare the genetic variability in three hatchery broodstocks of white cloud mountain minnow with the variability in six wild populations sampled in two river drainages. A total of 43 haplotypes in 102 specimens were observed, with no haplotype shared between wild and hatchery populations. The nucleotide diversity of the wild samples (0.048) was significantly higher than that of the hatche...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Isolation and characterisation of 26 microsatellite loci from a widespread tropical hydrozoan, Macrorhynchia phoenicea (Leptothecata, Aglaopheniidae), and cross-amplification in closely related species
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): B. Postaire, D. Aurelle, C.A.F. Bourmaud, J.H. Bruggemann, H. Magalon We isolated and characterized 26 microsatellite loci for Macrorhynchia phoenicea (Busk, 1852), a rather common tropical hydrozoan from the Indo-Pacific. The number of alleles per locus ranged from 4 to 24. The observed heterozygosity ranged from 0.000 to 0.970 and the expected heterozygosity from 0.029 to 0.833. Ten loci were at Hardy–Weinberg equilibrium. No pair of loci presented linkage disequilibrium. Transferability of up to 18 loci was posit...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Discovery of latitudinal gradient of triidothyronine concentrations in ectotherms as revealed from a cyprinid fish, the common roach Rutilus rutilus
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Boris A. Levin, Aleksey A. Bolotovskiy Important life history traits in groups of ectotherms have been shown to vary along a latitudinal axis. Despite sustained interest to this phenomenon, the underlying physiological mechanisms of latitudinal adaptation remain poorly understood. Thyroid hormones (THs) are key regulators of metabolism, development, and growth, and are involved in shaping adult phenotypes in lower vertebrates, fishes and amphibians. We tested the hypothesis that concentrations of triiodothyronine (T3), the m...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Phylogenetic analyses of the harvest mouse, Micromys minutus (Rodentia: Muridae) based on the complete mitogenome sequences
In this study, we determined the complete mitochondrial (mt) genome sequences of M. minutus, and used the complete mitochondrial genome sequences constructed the phylogenetic tree of Muroidea. The size of the genome is 16,232 bp in length and has a base composition of 33.6% A, 29.1% T, 24.8% C, and 12.5% G. The mitogenome structure was similar to that of typical vertebrate and other rodents' mitochondrial genomes, includes 13 protein-coding genes, 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, and 1 control region. We suggested a new initiation codon for ND5 (NADH dehydrogenase subunit), which has been never rep...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Genetic diversity and phylogenetic relationships among five endemic Pinus taxa (Pinaceae) of China as revealed by SRAP markers
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Qing Xie, Zhi-hong Liu, Shu-hui Wang, Zhou-qi Li The genetic diversity and phylogenetic relationships among five endemic Pinus taxa of China (Pinus tabulaeformis, P. tabulaeformis var. mukdensis, P. tabulaeformis f. shekanensis, Pinus massoniana and Pinus henryi) were studied by SRAP markers. Using 10 SRAP primer pairs, 247 bands were generated. The percent of polymorphic bands (94.8%), Nei's genetic diversity (0.2134), and Shannon's information index (0.3426) revealed a high level of genetic diversity at the genus-level. ...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Genetic diversity and the population structure at two ploidy levels of Lycoris radiata as revealed by SCoT analysis
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Yu-Xiang Liu, Tao Xia, Yu-hong Zheng, Yong-qi Zhi, Jian Zhou Ploidy differences can occur between and within species. To decipher the genetic structure of a species with different ploidy levels, we investigated an area of China where diploid and triploid individuals of Lycoris radiata co-occur. Twenty-six populations of L. radiata were sampled across its natural range, and SCoT markers were used to assess the genetic diversity and population structure. Chromosome counts revealed that 10 populations were diploid and 16 wer...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Analysis of genetic relationship on Amygdalus mira (koehne) Ricker with other peach species using simple sequence repeat (SSR)
In conclusion, A. mira maintains high genetic variation levels. This research will be potentially useful to aid breeding and enhance the economic and ornamental value of this wild peach. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

DNA barcoding and haplotyping in different species of Andrographis
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Rajender Goud Arolla, Neeraja Cherukupalli, Venkateswara Rao Khareedu, Dashavantha Reddy Vudem The genus Andrographis, belonging to the family Acanthaceae, contains several species of medicinal importance. Species, such as Andrographis alata, Andrographis echioides, Andrographis glandulosa, Andrographis lineata, Andrographis nallamalayana and Andrographis paniculata, with several bio-active compounds are being extensively used in folk medicine. However, difference of opinion exists with regard to inclusion of the species e...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Volatile constituents as complementary tools to characterize seven sardinian Genista species
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Alessandra Bertoli, Maria Cecilia Loi, Cecilia Noccioli, Lucia Maxia, Anna-Camilla Moonen, Luisa Pistelli The aroma fingerprints of seven endemic Sardinian populations of Genista species (G enista bocchierii, Genista arbusensis, Genista cadasonensis, Genista corsica, Genista morisii, Genista pichisermolliana, Genista sulcitana) were defined for the first time in order to investigate the relationship between their chemotaxonomic classification and discriminating volatiles. It is the first time that the inter-species compo...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Phylogenetic analysis of the genus Pseudoroegneria and the Triticeae tribe using the rbcL gene
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Jacques Gamache, Genlou Sun The Pseudoroegneria species are perennial grasses in the Triticeae tribe, whose St genome has been linked to several important polyploid species. Due to frequent hybridization and complex genetic mechanism, the relationships within Pseudoroegneria, and within the Triticeae have been heavily disputed. Using the chloroplast rbcL gene we estimated the nucleotide diversity of 8 Pseudoroegneria species. We also examined the phylogenetic relationships within Pseudoroegneria and of Pseudoroegneria within...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Isolation and characterization of microsatellite markers for funnel-eared bats Natalus mexicanus (Chiroptera: Natalidae) and cross-amplification using next-generation sequencing
This study presents the characterization of a series of 10 microsatellite loci developed for the funnel-eared bat Natalus mexicanus. A total of 35 pairs of primers, in 32 exemplars of N. mexicanus collected at two localities in Mexico, were selected by pyrosequencing for testing via cross-amplification in other bat species (Corynorhinus townsendii, Natalus major, Natalus tumidirostris, Nyctinomops laticaudatus and Tadarida brasiliensis). All 10 loci of this species amplified positively with medium and high resolution of genetic variation (2–15 alleles per locus, mean HE 0.7217, mean HO 0.6074). We consider that these...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

A novel sex-linked microsatellite marker for molecular sexing in rock bream fish Oplegnathus fasciatus
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Dongdong Xu, Bao Lou, Sanli Li, Xinxu Sun, Wei Zhan, Ruiyi Chen, Guomin Mao Sex-specific DNA markers can serve as efficient tools for molecular sex identification and thus provide important information for ecological and evolutionary studies, as well as for fishery management. In the present study, microsatellite markers were employed to identify sex-linked markers in the rock bream (Oplegnathus fasciatus). A microsatellite marker, designated as Oplfa16, displayed a male-specific genotype in rock bream. The male-specifi...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

The patterns of nitrogen and phosphorus stoichiometry across communities along altitudinal gradients in Qilian Mountains, China
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Shi-Jian Xu, Xiao-Yong Fan, Li-Long Wang, Xin-Fang Zhang, Li-Zhe An Unraveling the distribution patterns of plant nitrogen (N), phosphorus (P) stoichiometry along the elevational gradients has been attracting growing scientific interests of ecologists. However, little is known about patterns of plant root N, P stoichiometry along elevation and the relationship between foliar and root. Here we determined the foliar and root N, P concentrations and clarified the patterns of N:P stoichiometry across functional groups and com...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Population genetic structure and germplasm conservation of Stipa purpurea on Qinghai-Tibetan Plateau under grazing
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Xin-Ying Zhai, Yu-Xia Guo, Fu-Jiang Hou, Yang Liu, Deng-Tan Ma, Wei Wang, Xue-Bing Yan At what intensity the grazing should be practiced on the Qinghai-Tibetan Plateau in China is always confusing farmers, scientists and policy makers for a long time owing to its geographical exception. In order to develop a strategy for sustainable grazing management, we used the dominant Stipa purpurea as a model species to detect genetic diversity and fine spatial structure under different grazing intensities. Intra-population neutra...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

DNA barcode assessment of Mediterranean mayflies (Ephemeroptera), benchmark data for a regional reference library for rapid biomonitoring of freshwaters
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): Simone Cardoni, Roberta Tenchini, Irene Ficulle, Roberta Piredda, Marco Cosimo Simeone, Carlo Belfiore Accurate identification of aquatic species is fundamental to freshwater research. In this paper, we targeted Ephemeroptera, a key taxonomic insect group for biomonitoring of water bodies and present an overview on the efficacy of the DNA barcoding approach to document species identity in the Mediterranean region. We sequenced the mitochondrial cytochrome c oxidase (COI) in 39 nominal species. Sample discrimination and s...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research

Genetic analysis of the threatened American hart's-tongue fern (Asplenium scolopendrium var. americanum [Fernald] Kartesz and Gandhi): Insights into its mating system and implications for conservation
Publication date: October 2015 Source:Biochemical Systematics and Ecology, Volume 62 Author(s): D.D. Fernando, J.J. Discenza, J.R. Bouchard, D.J. Leopold American hart's-tongue fern (AHTF) is one of the rarest ferns in the United States and concern over its conservation and management has highlighted the need for genetic analysis. Genetic analysis also provides insights into the species' mating system which contributes to our understanding of its rarity and persistence. We analyzed 88 individuals from 11 populations in NY and MI based on variations in 108 loci as revealed through ISSR markers using Nei's gene diversi...
Source: Biochemical Systematics and Ecology - August 28, 2015 Category: Biochemistry Source Type: research