Diel variation of sesquiterpene elatol production: a chemical defense mechanism of the red seaweed Laurencia dendroidea
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Daniela Bueno Sudatti, Mutue Toyota Fujii, Silvana Vianna Rodrigues, Alexander Turra, Heitor Monteiro Duarte, Angélica Ribeiro Soares, Renato Crespo Pereira Variability in the production of secondary metabolites by seaweeds is commonly measured under natural conditions and is usually considered a response to environmental factors. However, it is not well known if levels of secondary metabolites can vary due to internal regulation of physiological processes. We measured the diel levels of elatol - the major defen...
Source: Biochemical Systematics and Ecology - January 11, 2016 Category: Biochemistry Source Type: research

Iridoids in Hydrangeaceae
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Chrysoula Gousiadou, Hong-Qing Li, Charlotte Gotfredsen, Søren Rosendal Jensen The content of glycosides in Kirengeshoma palmata and Jamesia americana (Hydrangeaceae) have been investigated. The former contains loganin and secoiridoids, including the alkaloid demethylalangiside. The latter contains no iridoids, but the known glucosides arbutin, picein and prunasin. In order to futher investigate the chemotaxonomy of the family Hydrangeaceae, the distribution of the iridoid and secoiridoid glucosides as well as the ...
Source: Biochemical Systematics and Ecology - December 23, 2015 Category: Biochemistry Source Type: research

Comparative genomics analysis of decapod shrimps in the Pancrustacea clade
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Jianbo Yuan, Xiaojun Zhang, Chengzhang Liu, Xiaoqing Sun, Elayaraja Sivaramasamy, Fuhua Li, Jianhai Xiang A reference genome is important for genomic and phylogenomic analysis, but except for a brachiopod Daphnia pulex, other crustacean genomes have not been completely sequenced. As one of the most economically important crustaceans, decapod shrimps have attracted great attentions worldwide. But the highly complexity block us from completing shrimp genomes. To explore the decapod shrimp genomes, we performed phylogenom...
Source: Biochemical Systematics and Ecology - December 23, 2015 Category: Biochemistry Source Type: research

Interspecific differences in chemical composition of femoral gland secretions between two closely related wall lizard species, Podarcis bocagei and Podarcis carbonelli
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Roberto García-Roa, Carlos Cabido, Pilar López, José Martín Chemical signals play an important role in intraspecific communication and social organization of many animals, but they also may be useful in interspecific recognition. In lizards, chemical signals are often contained in femoral gland secretions, of which composition may vary between species and populations. This may be especially important in recognition and reproductive isolation between closely related species. We analyzed by gas c...
Source: Biochemical Systematics and Ecology - December 20, 2015 Category: Biochemistry Source Type: research

Characterization of 15 microsatellite loci and genetic analysis of Heterodera schachtii (Nematoda: Heteroderidae) in South Korea
We report the development of fifteen polymorphic microsatellite markers and assess the genetic diversity and structure of three populations following a recent invasion of a previously unaffected region. Populations had low levels of heterozygosity, likely indicative of population structure, history, and inbreeding. Genetic diversity analysis suggested that the current infestation in South Korea may have come either from a single source population of mixed ancestry, or from multiple sources, indicating that implementing adequate prevention measures is still an unmet challenge. Much more work is needed on this species to ide...
Source: Biochemical Systematics and Ecology - December 18, 2015 Category: Biochemistry Source Type: research

Nucleotide polymorphism and phylogeographic history of an endangered conifer species Pinus bungeana
In this study, we detected the nucleotide polymorphism and phylogeographic history of P. bungeana based on three nuclear genes. We collected 16 natural populations and covered the entire distributional ranges of P. bungeana. We found that this endangered species have extremely low level of nucleotide diversity (π t = 0.00098, π sil = 0.00229) compared with that of other conifers. Meanwhile, hierarchical AMOVA revealed that genetic variations of two nuclear loci (010054_01 and 0_12329_02) mainly occurred among populations (54% and 55%), and another locus (0_14221_01) existed within populations (64%...
Source: Biochemical Systematics and Ecology - December 18, 2015 Category: Biochemistry Source Type: research

Genetic structure of wild rice Zizania latifolia and the implications for its management in the Sanjiang Plain, Northeast China
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Xiang-Rong Fan, Xu-Rui Ren, Yan-Ling Liu, Yuan-Yuan Chen The wild rice Zizania latifolia Turcz. is one of the dominant emergent species occurring in the wetlands of East Asia. Using sequence-related amplified polymorphism (SRAP) markers, genetic variation and population structure were assessed in nine Z. latifolia populations from the Sanjiang Plain, the second largest marsh in China. Five primer combinations produced a total of 344 unambiguous bands, of which 311 (90.4%) were polymorphic. A relatively low level of geneti...
Source: Biochemical Systematics and Ecology - December 18, 2015 Category: Biochemistry Source Type: research

Genetic diversity estimation and core collection construction of Sinojackia huangmeiensis based on novel microsatellite markers
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Jie Zhao, Yiqin Tong, Taiming Ge, Jiwen Ge Sinojackia huangmeiensis is a critically endangered tree species in the Styracaceae endemic to China. To create tools to better evaluate the genetic diversity of this species, we used a modified genomic library enrichment method, the PETUER (Probe Extension and TEACl Universal EnRichment) method, to develop genetic markers. The resulting 18 microsatellite loci had high polymorphism with 3–12 alleles per locus and showed negligible stutter. Observed (H O) heterozygosities we...
Source: Biochemical Systematics and Ecology - December 18, 2015 Category: Biochemistry Source Type: research

Chemical constituents from Ilex urceolatus
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): De-Qing Wang, Cheng-Nan Wu, Yang Song, Hai-Qiang Jiang, Hong-Lei Zhou, Chao Zhang, Hui-Fen Li, Xue-Lan Zhang, Yi Wu Twenty-four compounds were obtained from the extract of the leaves of I. urceolatus, which were divided into saturated fatty alcohols (1 and 2), triterpenoids (3–8 and 14–16), lignanoids (9, 20 and 22), coumarins (10 and 19), flavonoids (11–13, 21, 23 and 24) and others (17 and 18). Among them, compounds 1, 2, 17 and 18 were firstly obtained from the genus Ilex, others were isolated fr...
Source: Biochemical Systematics and Ecology - December 18, 2015 Category: Biochemistry Source Type: research

An analysis of genetic diversity in Marphysa sanguinea from different geographic populations using ISSR polymorphisms
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Huan Zhao, Yixiao Wang, Dazuo Yang, Xinda Zhao, Na Li, Yibing Zhou The genetic diversity of five wild populations of marine polychaete Marphysa sanguinea found in China was investigated using Inter-Simple Sequence Repeat-PCR (ISSR-PCR) polymorphisms. The results of the ISSR-PCR showed that 108 (90.8%) of the 119 ISSR loci tested were polymorphic. The Shannon's information index value was 0.4981, Nei's gene diversity was 0.3418, and the coefficient of gene differentiation (Gst) was 0.3671, which indicated that the among-...
Source: Biochemical Systematics and Ecology - December 11, 2015 Category: Biochemistry Source Type: research

Mitochondrial DNA and morphological analysis of hedgehogs (Eulipotyphla: Erinaceidae) in Algeria
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Louiza Derouiche, Rachid Bouhadad, Carlos Fernandes Algeria is the largest country in Africa and contains a rich and understudied faunal biodiversity. Two species of hedgehogs occur in the country: the North African hedgehog Atelerix algirus and the desert hedgehog Paraechinus aethiopicus. We investigated the genetic and phenotypic variation of the two species in Algeria using mitochondrial DNA and external morphological characters. The mitochondrial phylogenetic analysis identified two major clades corresponding to the tw...
Source: Biochemical Systematics and Ecology - December 9, 2015 Category: Biochemistry Source Type: research

The chemotaxonomic significance of phenylethanoid glycosides of Lathraea squamaria L. (Orobanchaceae)
In this study, we report the isolation and identification of two isomeric phenylethanoid glycosides, acteoside (1) and isoacteoside (2) that have not been previously described in this plant. Furthermore, unsubstituted benzoic acid (3) has been detected in the plant for the first time, and the presence of iridoid glycoside aucubin (4) was confirmed in all investigated plant parts. The chemotaxonomic significance of the determined compounds is discussed. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - December 2, 2015 Category: Biochemistry Source Type: research

Construction of a haustorium development associated SSH library in Thesium chinense and analysis of specific ESTs included by Imperata cylindrica
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Xiaoming Zhang, Bo Liu, Qiaosheng Guo, Lingshan Song, Lu Chen, Changlin Wang Thesium chinense Turcz. is a facultative hemiparasite that can invade host plants by inducing haustoria on neighboring host roots to access the water and nutrients of the host. Suppression subtractive hybridization (SSH) was performed to identify the differentially expressed genes during haustorial development. A total of 395 recombinants were randomly selected for sequencing. Two hundred seventy-six ESTs were retained after this screening proc...
Source: Biochemical Systematics and Ecology - December 1, 2015 Category: Biochemistry Source Type: research

Genetic structure and molecular divergence among samples of mandacaru (Cereus spp.; Cactaceae) as revealed by microsatellite markers
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Vanessa Neves de Azevedo Fernandes, Andréa Florindo das Neves, Paula Garcia Martin, Claudete Aparecida Mangolin, Maria de Fátima P.S. Machado Microsatellite transferability was used as a method to differentiate samples of mandacaru cultivated in the South (S), Southeast (SE), Northeast (NE) and Midwest (MW) regions of Brazil. Ten microsatellite primers designed from Polaskia chichipe (Pchi5, Pchi21, Pchi44 and Pchi47), Ariocarpus bravoanus (mAbR05, mAbR28 and mAbR86), Astrophytum asterias (AaB6 and AaD9), a...
Source: Biochemical Systematics and Ecology - December 1, 2015 Category: Biochemistry Source Type: research

Chemical composition of diaspores of the myrmecochorous plant Stemona tuberosa Lour
In conclusion, we suggest that hornets and ants obtain the essential nutrients from the elaiosomes of S. tuberosa and these rewards contribute to shape the hornet/ant-plant mutualism. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - December 1, 2015 Category: Biochemistry Source Type: research

Genetic and leaf-trait variability of Vinca minor at ancient and recent localities in Central Europe
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Petra Hlásná Čepková, Petr Karlík, Iva Viehmannová, Vladimíra Müllerová, Ladislav Šmejda, Michal Hejcman In Central Europe, Vinca minor has been planted for centuries as an ornamental, medicinal and ritual plant. We asked how variability in genetic and leaf traits of V. minor at ancient localities differs from that at recently established localities sampled mainly in the Czech Republic. Using selected ISSR primers, we obtained two clusters that correspond wel...
Source: Biochemical Systematics and Ecology - November 28, 2015 Category: Biochemistry Source Type: research

Preliminary analysis of the genetic diversity and population structure of mortiño (Vaccinium floribundum Kunth)
This study assessed the degree of genetic diversity and population structure of Ecuadorian V. floribundum as a preliminary step towards the establishment of effective conservation and sustainable-agriculture strategies. Mortiño individuals (126 in total) sampled from 3 regions in the northern highlands of Ecuador were characterized using 11 heterologous SSR markers originally developed for Vaccinium corymbosum. Expected heterozygosity (He = 0.49) revealed a moderate degree of genetic diversity for Ecuadorian mortiño, and pairwise F statistics between sampling regions (0.019≤ Fst ≤0.0...
Source: Biochemical Systematics and Ecology - November 28, 2015 Category: Biochemistry Source Type: research

Molecular phylogeny of the subfamily Schizothoracinae (Teleostei: Cypriniformes: Cyprinidae) inferred from complete mitochondrial genomes
In this study, we determined the complete mitochondrial genome of Schizopygopsis malacanthus. We also used the newly obtained sequence, together with 31 published schizothoracine mitochondrial genomes that represent eight schizothoracine genera and six outgroup taxa to reconstruct the phylogenetic relationships of the subfamily Schizothoracinae by different partitioned maximum likelihood and partitioned Bayesian inference at nucleotide and amino acid levels. The schizothoracine fishes sampled form a strongly supported monophyletic group that is the sister taxon to Barbus barbus. A sister group relationship between the prim...
Source: Biochemical Systematics and Ecology - November 27, 2015 Category: Biochemistry Source Type: research

Halogenated eudesmane derivatives and other terpenes from the marine red alga Laurencia pinnata and their chemotaxonomic significance
Publication date: February 2016 Source:Biochemical Systematics and Ecology, Volume 64 Author(s): Nai-Yun Ji, Xiao-Ming Li, Lan-Ping Ding, Bin-Gui Wang Chemical investigation of the marine red alga Laurencia pinnata collected from Nanji Island of China resulted in the isolation and identification of three new eudesmane (selinane) sesquiterpenes, 1β-bromoselin-11-en-4α-ol (1), 1β-bromo-4α,5α-epoxyselinane (2), and 1β-bromoselin-3,11-diene (3), and one new 6,8-cycloeudesmane sesquiterpene, 1β-bromo-6,8-cycloselin-4(15)-ene (4), together with one known eudesmane sesquiterpene, 1&beta...
Source: Biochemical Systematics and Ecology - November 27, 2015 Category: Biochemistry Source Type: research

Phylogeny of section Leuce (Populus, Salicaceae) inferred from 34 chloroplast DNA fragments
This study provides an important foundation for evolutionary studies and for the effective utilization of the wild germplasm resources of section Leuce. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - November 2, 2015 Category: Biochemistry Source Type: research

De novo assembly and characterization of transcriptome using Illumina sequencing and development of twenty five microsatellite markers for an endemic tree Juglans hopeiensis Hu in China
In this study, more than 44 million sequencing reads were generated using Illumina sequencing technology. De novo assembly yielded 93,822 unigenes with an average length of 731 bp. Based on sequence similarity search with known proteins, a total of 39,708 (42.3%) genes were identified. Searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG) indicated that 15,903 (17.0%) unigenes. To contribute to its conservation and management, twenty five microsatellite markers were identified in J. hopeiensis were screened for polymorphism across 27 Chinese walnut tree individuals from two locations. T...
Source: Biochemical Systematics and Ecology - November 2, 2015 Category: Biochemistry Source Type: research

Esters and other constituents of the foliar cuticular wax of a soybean variety
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): C.E.J. Palacios, G. Negri, A. Salatino The constituents of the foliar cuticular wax of the variety ‘MG/BR 46 Conquista’ were isolated and chemically characterized. N-Alkanes, esters and triterpenoids are major constituents of the cuticular wax. Esters, taraxerol and steroids seem to be valuable cuticular wax constituents for characterization and distinction among soybean varieties. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - November 2, 2015 Category: Biochemistry Source Type: research

Phylogeography of the temperate tree species Quercus acutissima in China: Inferences from chloroplast DNA variations
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Xingwang Zhang, Yao Li, Chengyuan Liu, Tao Xia, Qiang Zhang, Yanming Fang Quercus acutissima is one of the most widespread temperate deciduous tree species in China. To study its phylogeographical pattern and demographic history, three chloroplast DNA fragments (atpB-rbcL, psbA-trnH and trnS-trnG) from 401 individuals representing 30 populations were sequenced. A total of 19 haplotypes were identified, and these showed a weak phylogeographical structure (N ST = 0.689 > G ST = 0.630, ...
Source: Biochemical Systematics and Ecology - November 2, 2015 Category: Biochemistry Source Type: research

Development of EST-SSR markers and analysis of genetic diversity in natural populations of endemic and endangered plant Phoebe chekiangensis
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Yajun Ding, Junhong Zhang, Yunfeng Lu, Er'pei Lin, Luhuan Lou, Zaikang Tong Phoebe chekiangensis is a uniquely precious timber and ornamental tree species of Southern China that enjoys nationwide protection as an endangered species. In the present study, we identified a total of 22,985 SSR loci based on transcriptome sequencing data, and 41 polymorphic markers were chosen to analyze the genetic diversity of seven natural populations including 190 individuals. Results showed that a moderate level of genetic diversity was...
Source: Biochemical Systematics and Ecology - November 2, 2015 Category: Biochemistry Source Type: research

Microsatellite DNA reveals genetically different populations of Atlantic bonito Sarda sarda in the Mediterranean Basin
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Cemal Turan Microsatellites were used to investigate population genetic structure of Atlantic bonito Sarda sarda from the Black Sea, Marmara Sea, Aegean Sea, north-eastern Mediterranean Sea and Adriatic Sea. Overall average observed heterozygosity was high (0.93). Average observed heterozygosity per locus ranged from 0.79 to 0.98. Pairwise FST estimates for all loci between populations ranged from 0 to 0.07626, and significant FST values (P < 0.001) were detected between populations; the Blacks Sea and Marma...
Source: Biochemical Systematics and Ecology - October 27, 2015 Category: Biochemistry Source Type: research

Development of microsatellites for the genus Salamandrina: A tool to discriminate between northern and southern spectacled salamanders (Salamandrina perspicillata and Salamandrina terdigitata) and their hybrids
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Alessandra Ventura, Claudio Ciofi, Chiara Natali, Marco Mattoccia Ten microsatellite loci were developed for the northern spectacled salamander, Salamandrina perspicillata, and cross-amplification was obtained for the southern spectacled salamander, Salamandrina terdigitata. These two species are the sole representative of the genus Salamandrina, a threatened amphibian endemic to the Italian peninsula. The two salamanders show a parapatric distribution with a contact zone in Central Italy. All but one locus were polymorph...
Source: Biochemical Systematics and Ecology - October 27, 2015 Category: Biochemistry Source Type: research

Fifteen new polymorphic microsatellite loci for the meadow brown butterfly, Maniola jurtina
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Murielle Richard, Anne Villemey, Virginie M. Stevens, Gaelle Blanvillain, Sophie Dardenne, Michel Baguette We characterized fifteen microsatellite markers for the butterfly Maniola jurtina. For the six studied populations (96 samples) the total number of alleles per locus ranged from 3 to 55 and mean overall expected heterozygosity across all loci was 0.74. In spite of a high frequency of null alleles detected in part of the loci, a recurrent phenomenon in Lepidopteron, the estimation of pairwise FST seems rather insens...
Source: Biochemical Systematics and Ecology - October 27, 2015 Category: Biochemistry Source Type: research

Genetic diversity and population structure of Taxus cuspidata in the Changbai Mountains assessed by chloroplast DNA sequences and microsatellite markers
We examined one chloroplast DNA (cpDNA) region (petA-psbE) and 9 nuclear simple sequence repeats (SSRs) loci variations among seven populations in the Changbai Mountains of China to investigate the levels of genetic diversity and population structure. A moderate level of haplotype diversity (H T = 0.625), low nuclear microsatellite diversity (HE = 0.261 ± 0.028), significant genetic differences (F ST = 0.065) and substantial gene flow (Nm = 2.806) were observed. Most of the total genetic variation was partitioned within the population (87.8% and 94.0% for cpDNA and S...
Source: Biochemical Systematics and Ecology - October 27, 2015 Category: Biochemistry Source Type: research

Chemical constituents from the roots of Clerodendrum indicum and Clerodendrum villosum
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Pathom Somwong, Masataka Moriyasu, Rutt Suttisri Four triterpenoids (1–4), nine steroids and steroid glycosides (6–11, 13–15) and two flavonoids (17–18) were isolated from the roots of Clerodendrum indicum, whereas three triterpenoids (1, 4 and 5), six steroids and steroid glycosides (9–10, 12–14, 16) and one phenylethanoid glycoside (19) were obtained from the roots of Clerodendrum villosum. This is the first report of chemical constituents of C. villosum, and the first report of compou...
Source: Biochemical Systematics and Ecology - October 26, 2015 Category: Biochemistry Source Type: research

Temporal and spatial dynamics of phytoplankton diversity in the East China Sea near Jeju Island (Korea): A pyrosequencing-based study
In this study, the community structure and diversity of phytoplankton in the northern part of the ECS adjacent to Jeju Island were explored using small subunit ribosomal RNA (SSU) pyrosequencing. We analysed samples collected from four stations from the surface and at 30-m and 50-m depths during April and September 2011. We observed spatial and temporal variations in the phytoplankton community. Among phytoplankton, diatoms and dinoflagellates constituted a major portion at all stations (60–90%). However, comparison of the April and September samples showed seasonal variation and shifts in the dispersion of diatom an...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Population structure of large yellow croaker (Larimichthys crocea) revealed by single nucleotide polymorphisms
In this study, Sequenom MassARRAY was used to analyze 120 individuals from three geographic populations in the East and South China Seas and a cultured stock. Forty-four of 60 single nucleotide polymorphism (SNP) loci were ascertained. Eleven loci, scf117, scf708, scf247, scf595, scf100, scf166, scf305, scf664, scf117, scf187 and scf511 each showed population-specific genotypes. A total of 127 alleles were detected. Averagely, the effective number of alleles was between 1.328 and 1.341, expected heterozygosity and observed heterozygosity ranged from 0.273 to 0.320, and 0.190 to 0.235, respectively, Pic value ranged from 0....
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Characterization of microsatellites and repetitive flanking sequences (ReFS) from the topmouth culter (Culter alburnus Basilewsky)
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Shi-Li Liu, Zhi-Min Gu, Jin-Liang Zhao, Yong-Yi Jia, Wen-Ping Jiang, Jian-Lin Guo, Qian Li The topmouth culter (Culter alburnus) is an economically important freshwater fish in China. We obtained 159 microsatellite containing sequences (MCSs) from genomic DNA in this species enriched by (CAA)8 and (GAA) 8 probes. Careful examination of these sequences revealed the existence of cryptic repeated elements on presumed unique flanking regions. These cryptic elements can be grouped into three families, with the MCSs of the e...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

From population ecology to metabolism: growth of Trypanosoma evansi, and implications of glucose depletion, in a live host
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): S. Andrea Moreno, Jesús Molinari, Mayerly Nava Little attention has been paid to the potential top-down effects of population ecology on metabolism. Because it is capable of a fast growth that dramatically modifies its blood environment, the parasitic protozoan, Trypanosoma evansi, is a promising model for the study of density-dependent metabolic processes. We assess the in vivo growth rate, doubling time, biomass yield, glycolytic flux, and enzyme expression of T. evansi. Then, we explore the metabolic changes...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Genetic diversity and population structure analysis in wild strawberry (Fragaria nubicola L.) from Motuo in Tibet Plateau based on simple sequence repeats (SSRs)
In this study, we assessed genetic diversity of seventy wild strawberry germplasms from different geographical regions using simple sequence repeat (SSR) markers. The genetic diversity of wild strawberry was demonstrated by 189 polymorphic SSR-PCR bands obtained using ten selective primers. Additionally, the average polymorphic information content (PIC), total gene diversity and population diversity were 0.941, 0.331 and 0.214, respectively. At the population level, variation of strawberry accessions among population was higher than that within population. Based on arithmetic mean (UPGMA) dendrogram method, all samples wer...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Characterization of 10 new tetranucleotide microsatellite markers for the European eagle owl, Bubo bubo: Useful tools for conservation strategies
We report on the isolation and development of 10 new microsatellites for the Eagle owl, B. bubo. All loci (10 tetra-nucleotide) are characterized by high polymorphism levels. Number of alleles ranged from 5 to 13 and expected heterozygosity varied from 0.733 to 0.840. These microsatellites would be very useful to assess the genetic diversity, connectivity patterns and parentage of B. bubo. This information will allow to establish new conservation strategies and improve the management of the species. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Temperature niche conservatism and strong genetic structure are involved in the trans-Panamanian colonization of Matudaea (Hamamelidaceae) to Andean forests
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Gustavo Adolfo Silva-Arias, Favio González, Sonia Tinjacá, María Isabel Chacón Sánchez Matudaea is the only genus of the Hamamelidaceae found in South America. The genus is composed by two extant species, M. trinervia, from Mexico and Costa Rica, and Matudaea colombiana, from the Colombian Andes; additional fossil records are present in Central Europe. Population genetics, molecular phylogenetics and niche modelling approaches were applied to explain processes related with the trans-Pana...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Ultra-performance liquid chromatography fingerprint combined with chemometrics as an effective strategy for Dianbaizhu species discrimination
The objective was to develop a precise and reproducible method to differentiate among these species. A method was developed by using ultra-performance liquid chromatography in combination with chemometric. The method was validated with relative standard deviations of less than 1.96%. Graphical abstract (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Taxonomy, phylogenetics and biogeography of Chesneya (Fabaceae), evidenced from data of three sequences, ITS, trnS-trnG, and rbcL
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Ming-Li Zhang, Zhi-Bin Wen, Xiao-Li Hao, Vyacheslav V. Byalt, Alexander P. Sukhorukov, Stewart C. Sanderson Plants of Central Asia have played a significant role in the origin of floras of Eurasia and the Northern Hemisphere. Chesneya, a small leguminous genus occurring in Central Asia, western Asia, and Tibet, is used to establish phylogenetic relationships and discuss the evolutionary and biogeographical history based on sequence data of ITS and trnS-trnG and rbcL. We employed BEAST Bayesian inference for dating, and ...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Changes in antioxidant defense system in gills of Capoeta umbla caught from Uzuncayir Dam Lake, Turkey
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Durali Danabas, Nuran Cikcikoglu Yildirim, Numan Yildirim, Ayten Oztufekci Onal, Gulsad Uslu, Erhan Unlu, Seval Danabas, Cemil Ergin, Nilgun Tayhan The aim of this work was to determine the seasonal changes in the activities of selected biomarkers in Capoeta umbla (Heckel, 1843) caught from Uzuncayir Dam Lake (Tunceli, Turkey) and to evaluate the effects of environmental factors on these activities. Fish were sampled on seasonal basis, and superoxide dismutase, catalase, glutathione peroxidase activities and levels o...
Source: Biochemical Systematics and Ecology - October 23, 2015 Category: Biochemistry Source Type: research

Phenylpropanoids from Paspalum atratum (Poaceae)
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Luciano da Silva Pinto, Francisco Humberto Dübbern de Souza, Isabele Rodrigues Nascimento, Lucia Maria Xavier LopesGraphical abstract (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - October 9, 2015 Category: Biochemistry Source Type: research

Genetic diversity of Lotus corniculatus in relation to habitat type, species composition and species diversity
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Eleni M. Abraham, Ioannis Ganopoulos, Panagiota Giagourta, Maslin Osathanunkul, Irene Bosmali, Athanasios Tsaftaris, Athanassios Papaioannou, Panagiotis Madesis The genetic variability of Lotus corniculatus, a common and important fodder legume, was studied in relation to habitat type and to species diversity at a local level. The study was conducted in Cholomontas mountain, northern Greece, at altitudes of 760–870 m. Genetic material was selected from four forested, at the edge of forest, and four open gra...
Source: Biochemical Systematics and Ecology - October 9, 2015 Category: Biochemistry Source Type: research

Enhanced emissions of floral volatiles by Diplotaxis erucoides (L.) in response to folivory and florivory by Pieris brassicae (L.)
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Gerard Farré-Armengol, Iolanda Filella, Joan Llusia, Clara Primante, Josep Peñuelas The main function of floral emissions of volatile organic compounds (VOCs) in entomophilous plants is to attract pollinators. Floral blends, however, can also contain volatile compounds with defensive functions. These defensive volatiles are specifically emitted when plants are attacked by pathogens or herbivores. We characterized the changes in the floral emissions of Diplotaxis erucoides induced by folivory and florivory b...
Source: Biochemical Systematics and Ecology - October 9, 2015 Category: Biochemistry Source Type: research

Molecular dating of a marine species, Eriocheir japonica (Decapoda: Grapsidae), corroborates cenozoic tectonic events and sea level fluctuation
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Daizhen Zhang, Ge Ding, Baoming Ge, Huabin Zhang, Boping Tang Changes of coastal topography for Cenozoic Himalayan orogeny complicated the phylogeographical structure of marine species and deepened their divergences. To test the association between divergence and Cenozoic tectonic events, we analyzed the phylogeographical structure of Eriocheir japonica by combining molecular data and geographical environment events. The four distinct lineages obtained through phylogenetic reconstruction and network analysis demonstrated...
Source: Biochemical Systematics and Ecology - October 8, 2015 Category: Biochemistry Source Type: research

Analysis of genetic relations and evaluation of medicinal value among Hedera plants in China by sequence related amplified polymorphism markers and high performance liquid chromatography detection
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Hua-peng Sun, Fang Li, Xiong-jun Cao, Qin-mei Ruan, Xiao-hong Zhong Hedera plants are ornamental vines which are regarded as traditional medicinal plants. These plants are widely cultivated worldwide; moreover, new species and varieties have been discovered. For the first time, molecular markers based on sequence-related amplified polymorphism (SRAP) were used in this study to classify species and to analyse the genetic relationship of 21 Hedera plant varieties which are commonly cultivated in China. We also employed hig...
Source: Biochemical Systematics and Ecology - October 2, 2015 Category: Biochemistry Source Type: research

Diterpenes and kawalactone from the rhizomes of Amomum uliginosum J.Koenig
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Wannaporn Chate, Nuchnipa Nuntawong Seven compounds, including three labdane diterpenes (1, 4, 6), one bisnorlabdane diterpene (2), one steroid (3), one sesquiterpene (5) and one kawalactone (7) were isolated from the rhizomes of Amomum uliginosum J.Koenig. Compounds 1–4, 6 and 7 were firstly isolated from the genus Amomum. This work represented the first phytochemical study on this plant. The chemotaxonomic significance of these compounds was summarized. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - October 2, 2015 Category: Biochemistry Source Type: research

Genetic diversity and genetic structure of Corydalis tomentella Franch. (Papaveraceae), an endangered herb species from Central China
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Zhong-Xin Zhang, Hong-Yu Niu, Xuan Guo, Dong Wang, William D. Eaton Corydalis tomentella Franch. (Papaveraceae), a species endemic to Central China, occurs within specialized habitats of limestone cliffs. To date, extensive harvesting by local people for medical use has led to a sharp decline in wild populations of this plant. To investigate the genetic diversity and genetic structure of the species, differences in the composition of one nuclear DNA (nDNA) locus (NADPH) and two chloroplast DNA (cpDNA) fragments (trnH-psb...
Source: Biochemical Systematics and Ecology - October 2, 2015 Category: Biochemistry Source Type: research

Development of EST-SSR markers for Taxus cuspidata from publicly available transcriptome sequences
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Saneyoshi Ueno, Yafeng Wen, Yoshihiko Tsumura Simple sequence repeats (SSRs) from a public database were surveyed to identify potential EST-SSR markers for Taxus cuspidata. A total of 5.4 gigabase pairs of RNA-sequencing data were downloaded and assembled into 179,687 contigs, 7404 (4.1%) of which contained SSR sequences. One hundred and fifty PCR primer pairs were designed for SSRs with repeat numbers ≥9 and screened for variation in 18 T. cuspidata trees. Eighty markers showed length variation, with numbers of alleles...
Source: Biochemical Systematics and Ecology - September 30, 2015 Category: Biochemistry Source Type: research

Cadinane-type sesquiterpenoids from Schisandra bicolor
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Yinning Chen, Heying Zhou, Xinhong Liu, Zhifang Xu, Samuel X. Qiu, Riming Huang The phytochemical investigation on the stems of Schisandra bicolor led to the isolation of seven cadinane-type sesquiterpenoids, 2-hydroxy-11,12-dehydrocalamenene (1), ent-T-muurolol (2), (+)-ent-epicubenol (3), (1S,4S)-7,8-dihydroxy-11,12-dehydrocalamenene (4), cadinane-T-cadinol (5), (−)-cadin-4,10(15)-dien-11-oic acid (6), and cadina-4,10(15)-dien-3-one (7). Their structures were determined by extensive analysis of their spectroscop...
Source: Biochemical Systematics and Ecology - September 27, 2015 Category: Biochemistry Source Type: research

Transcriptome derived microsatellite markers of Tetraclita kuroshioensis and cross amplification among Tetraclita spp.
In this study, eight microsatellite markers were isolated from Tetraclita kuroshioensis through transcriptome sequencing. A total of three to eight alleles were revealed from each locus. The expected and observed heterozygosities ranged from 0.433 to 0.933 and from 0.505 to 0.762, respectively. Cross amplification among five Tetraclita barnacle species found in the Indo-West Pacific was successful with all eight microsatellite markers, although success rate varied among species. Therefore, the microsatellite markers developed in this study can be useful tools in future genetic studies with tetraclitid barnacles. (Source: B...
Source: Biochemical Systematics and Ecology - September 27, 2015 Category: Biochemistry Source Type: research

Assessment of genetic diversity of Czech sweet cherry cultivars using microsatellite markers
Publication date: December 2015 Source:Biochemical Systematics and Ecology, Volume 63 Author(s): Kamal Sharma, Haibo Xuan, Petr Sedlák We analyzed 24 sweet and wild cherry genotypes collected in Czech Republic to determine genetic variation, using previously described 16 SSR primers to adapt a fast, reliable method for preliminary screening and comparison of sweet cherry germplasm collections. All SSRs were polymorphic and they were able all together to distinguish unambiguously the genotypes. These SSR primers generated 70 alleles; the number of alleles per primer ranged from 2 to 7, with a mean of 4.4 putativ...
Source: Biochemical Systematics and Ecology - September 27, 2015 Category: Biochemistry Source Type: research