Chloroplast phylogenomic analyses maternal relationships among sections in the genus Populus
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Limei Zhang, Ce Shang, Fang K. Du, Fengbin Zhao, Biao Xiong, Zhixiang Zhang The genus Populus L. has been divided into five sections based on morphological characters, but the phylogenetic relationships among sections remain uncertain. Topological discrepancies have been reported between trees obtained using nuclear and plastid sequences. We selected nine chloroplast genomes from all five sections, including two new sequenced species in this study for analyses of maternal phylogenetic relationships in the genus Populus at the ...
Source: Biochemical Systematics and Ecology - November 19, 2016 Category: Biochemistry Source Type: research

Identification of species-specific microsatellite markers in three Siniperca species by RNA-Seq
In this study, a total of 991 SSR primer pairs were identified in 8923 SSR-containing sequences (transcripts >1 kb) by RNA-Seq. Then, 566 loci were randomly selected for primer synthesis and 394 of which were successfully amplified in S. chuatsi. All the non-polymorphic loci (308) were evaluated among three Siniperca species, and 18 species-specific microsatellite markers were finally validated among three Siniperca species. These species-specific microsatellite markers would not only easily discriminate these three Siniperca species at juvenile stage, but also effectively monitor the statuses of hybridization ...
Source: Biochemical Systematics and Ecology - November 17, 2016 Category: Biochemistry Source Type: research

The complete mitochondrial genome of Japanese sparrowhawk (Accipiter gularis) and the phylogenetic relationships among some predatory birds
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Gang Liu, Chao Li, Youwen Du, Xiaoying Liu Phylogenetic relationships among raptors, especially various groups are rather complex and controversial. We determined the complete mtDNA of Japanese sparrowhawk, and estimated phylogenetic trees based on the complete mtDNA alignment of it and 36 other raptor species, to clarify raptor phylogenetics. Phylogenetic trees were also estimated using a multiple sequence alignment of 12S rRNA and 16S rRNA from 81 typical species in GenBank, to further clarify the phylogenetic relationships ...
Source: Biochemical Systematics and Ecology - November 17, 2016 Category: Biochemistry Source Type: research

Altitudinal changes in secondary metabolite contents of Hypericum androsaemum and Hypericum polyphyllum
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Cuneyt Cirak, Jolita Radusiene, Valdas Jakstas, Liudas Ivanauskas, Fatih Seyis, Fatih Yayla The genus Hypericum (Hypericaceae) has attracted scientific interest as its members have yielded many bioactive compounds. In the present study we investigated the content of hypericin, pseudohypericin, hyperforin, adhyperforin, chlorogenic acid, neochlorogenic acid, caffeic acid, 2,4-dihydroxybenzoic acid, 13,II8-biapigenin, hyperoside, isoquercitrin, quercitrin, quercetin, avicularin, rutin, (+)-catechin and (−)-epicatechin in a...
Source: Biochemical Systematics and Ecology - November 13, 2016 Category: Biochemistry Source Type: research

Molecular phylogenetic status of the Bulgarian marbled polecat (Vormela peregusna, Mustelidae, Carnivora), revealed by Y chromosomal genes and mitochondrial DNA sequences
In this study, we investigated molecular phylogenetic status of the marbled polecat (Vormela peregusna) from Bulgaria, using sequences of two Y-chromosomal genes (SRY and ZFY). The phylogenetic tree inferred using combined sequences of both genes indicated that the marbled polecat was split from genera Lutra, Neovison and Mustela after genus Martes was diverged in family Mustelidae. In addition, we analyzed molecular phylogeography of the Bulgarian population of the marbled polecat, using cytochrome b and control region sequences of mitochondrial DNA (mtDNA). The phylogenetic tree of cytochrome b indicated that the haploty...
Source: Biochemical Systematics and Ecology - November 12, 2016 Category: Biochemistry Source Type: research

Chemical constituents from the leaves of Cinnamomum parthenoxylon (Jack) Meisn. (Lauraceae)
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Xuan Wei, Guo-Hui Li, Xiao-Ling Wang, Ji-Xiang He, Xiao-Ning Wang, Dong-Mei Ren, Hong-Xiang Lou, Tao Shen Phytochemical investigation of the ethanolic extract from the leaves of Cinnamomum parthenoxylon (Jack) Meisn. led to the isolation of (3R, 4R, 3′R, 4′R)-6,6′-dimethoxy-3, 4, 3′, 4′-tetrahydro-2H, 2′H-[3, 3′]bichromenyl-4, 4′-diol (1), 4-hydroxybenzaldehyde (2), 1,2,4-trihydroxybenzene (3), kaempferol-3-O-α-l-rhamnoside (4), herbacetin (5), quercetin-3-O-α-l-rhamn...
Source: Biochemical Systematics and Ecology - November 12, 2016 Category: Biochemistry Source Type: research

Molecular phylogeny of the genus Capoeta (Teleostei: Cyprinidae) in Anatolia, Turkey
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Yusuf Bektas, Davut Turan, Ismail Aksu, Yılmaz Ciftci, Oguzhan Eroglu, Gokhan Kalayci, Ali Osman Belduz Phylogeny of Capoeta genus distributed in Anatolia were carried out by analysing mitochondrial cytochrome b gene (1140 bp) sequences from 332 samples representing 59 populations of 15 species across their geographical distribution. Haplotype network and phylogenetic analysis (neighbor-joining, maximum-likelihood, maximum parsimony, and bayesian inference) of the 103 cytochrome b haplotypes detected in Capoeta species r...
Source: Biochemical Systematics and Ecology - November 12, 2016 Category: Biochemistry Source Type: research

Isozyme analysis of genetic variability and population structure of Lactuca L. germplasm
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Mohamed A. El-Esawi, Abd El-Zaher Mustafa, Salwa Badr, Reda Sammour Isozymes were used to investigate the genetic variability, population structure, and relationships of Lactuca germplasm. The isozyme systems revealed 16 putative loci of a total of 31 alleles. Out of these 16 loci, 11 were polymorphic. The average values of expected heterozygosity (H e), observed heterozygosity (H o), mean number of alleles per locus (A) and effective number of alleles per locus (A e) were 0.2227, 0.266, 1.3005 and 1.369, respectively. The ave...
Source: Biochemical Systematics and Ecology - November 11, 2016 Category: Biochemistry Source Type: research

Phytochemical study of Joannesia princeps Vell. (Euphorbiaceae) leaves
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): César Muñoz Camero, Abeer Temraz, Alessandra Braca, Marinella De Leo A phytochemical study of chloroform-methanol and methanol extracts of Joannesia princeps Vell. Leaves led to the isolation of twenty eight compounds, including two α-ionones (2, 5), three glycosylated monoterpenes (1, 3, 4), eight phenolic compounds (6, 8, 9, 12, 14, 17, 18, 24), two gallotannins (10, 11), twelve flavonoids (7, 15, 16, 19, 20–23, 25–28), and one lignan (13). The structural characterization of the isolated compo...
Source: Biochemical Systematics and Ecology - November 11, 2016 Category: Biochemistry Source Type: research

Bioinformatic analysis of gene encoding odorant binding protein (OBP) 1, OBP2, and chemosensory proteins in Grapholita molesta
In conclusion, GmolOBP1 and GmolOBP2 shared some common properties with other OBPs. Additionally, GmolOBP1, GmolOBP2, and GmolCSP may have evolved independently. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - November 7, 2016 Category: Biochemistry Source Type: research

Morphological comparison and DNA barcoding of four closely related species in the genera Misgurnus and Paramisgurnus (Cypriniformes: Cobitidae)
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Shaokui Yi, Jia Zhong, Songqian Huang, Sai Wang, Weimin Wang To evaluate the feasibility of morphological and genetic identification of the closely related species in the genera Misgurnus and Paramisgurnus, the morphological characters of four species in these genera and DNA barcoding of five loaches (P. dabryanus, M. anguillicaudatus, M. bipartitus, M. mohoity, and Barbatula toni) were investigated. Twelve morphological characters were measured in 542 individuals to perform the comparative analysis. Among these characters, on...
Source: Biochemical Systematics and Ecology - November 5, 2016 Category: Biochemistry Source Type: research

Complete mitochondrial genome of ring-necked pheasant  Phasianus colchicus alaschanicus from China's Helan Mountains and description of its phylogenetic relationships in Galliformes
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Chang Zhao, Hui Gao, Yujiao Sun, Zhensheng Liu, Liwei Teng Phasianus colchicus alaschanicus, Phasianidae, Galliformes is known only from the Helan Mountains of Ningxia and Inner Mongolia Autonomous Regions, China. Due to few studies on this subspecies, the taxonomic status of Phasianus colchicus alaschanicus in Galliformes is unclear. We sequenced the mitochondrial genome of one P. c. alaschanicus and downloaded 36 species of 22 genera from GeneBank to construct a phylogenetic tree based on the Maximum Likelihood (ML) method. ...
Source: Biochemical Systematics and Ecology - November 5, 2016 Category: Biochemistry Source Type: research

3D chemoecology and chemotaxonomy of corals using fatty  acid biomarkers: Latitude, longitude and depth
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Cátia Figueiredo, Miguel Baptista, Inês C. Rosa, Ana Rita Lopes, Gisela Dionísio, Rui J.M. Rocha, Igor C.S. Cruz, Ruy K.P. Kikuchi, Nuno Simões, Miguel Costa Leal, Inês Tojeira, Narcisa Bandarra, Ricardo Calado, Rui Rosa With the objective of uncovering differences in the fatty acid (FA) composition of hexa- and octocorals from different climatic zones (equatorial, subtropical and tropical) and distinct habitats (e.g. rock and coral reefs; intertidal to deep-sea environments), the FA compositio...
Source: Biochemical Systematics and Ecology - November 5, 2016 Category: Biochemistry Source Type: research

Molecular cloning reveals co-occurring species behind red  tide blooms of the harmful dinoflagellate Cochlodinium polykrikoides
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Ponmani Thangaraj, Tae Gyu Park, Jang-Seu Ki Red tides caused by the marine dinoflagellate Cochlodinium polykrikoides Margalef pose significant environmental problems worldwide. Recently, the existence of severe blooms attributable to a single Cochlodinium Schütt species has been questioned by many researchers. Herein we investigated the dinoflagellate composition of harmful algal blooms (HABs) attributed to C. polykrikoides in Korean coastal waters at nine different stations (St.). The component species of Cochlodinium b...
Source: Biochemical Systematics and Ecology - November 5, 2016 Category: Biochemistry Source Type: research

Low population differentiation revealed in the highly threatened elongate loach (Leptobotia elongata, Bleeker), a  species endemic to the fragmented upper reaches of the Yangtze River
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Hong-Yan Liu, Fei Xiong, Xin-Bin Duan, Hui-Wu Tian, Shao-Ping Liu, Da-Qing Chen The elongate loach (Leptobotia elongata) is a typical migratory fish that was formerly found in abundance throughout the upper and middle reaches of the Yangtze River but is now classified as a highly threatened fish on the China Species Red List. Here, a comparative study of microsatellite loci was performed to evaluate the genetic diversity and analyze the population structure in seven locations in the upper reaches of the Yangtze River, China. T...
Source: Biochemical Systematics and Ecology - November 4, 2016 Category: Biochemistry Source Type: research

Chemical variability in volatile composition between several Italian accessions of Siler montanum (S. montanum subsp. montanum and S. montanum subsp. siculum)
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Filippo Maggi, Fabrizio Bartolucci, Fabio Conti Laserpitium siler, currently recognized as Siler montanum, is a polymorphic species belonging to the Apiaceae family and distributed in mountains of Southern Europe. In the present work we have analysed five accessions from Italy (Piemonte, Valle d'Aosta and Abruzzo) belonging to S. montanum subsp. montanum and subsp. siculum for the essential oil composition with the aim to find correlations between chemical data and taxonomic relationships. Results, obtained by gas chromatograp...
Source: Biochemical Systematics and Ecology - November 4, 2016 Category: Biochemistry Source Type: research

Chemotaxonomic value of flavonoids in Chromolaena congesta (Asteraceae)
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): Josiane Aline Monteiro de Oliveira, Darlon Irineu Bernardi, Rodolfo Bento Balbinot, Alexandre da Silva Avíncola, Eduardo Pilau, Marta Regina Barrotto do Carmo, Maria Helena Sarragiotto, Debora Cristina Baldoqui The phytochemical investigation on the aerial parts of Chromolaena congesta led to the isolation of nine flavonoids, known in the literature as genkwanin (1) kumatakenin (2) acacetin (3), kaempferol 3-methyl ether (4), apigenin (5), apigenin 5,7-dimethyl ether (6), apigenin 5-methyl ether (7), luteolin (8) and ka...
Source: Biochemical Systematics and Ecology - November 4, 2016 Category: Biochemistry Source Type: research

Genetic diversity of golden takin (Budorcas taxicolor bedfordi) population from Qinling Mountains in China revealed by sequence analysis of mitochondrial DNA control region
Publication date: February 2017 Source:Biochemical Systematics and Ecology, Volume 70 Author(s): H. Feng, C.L. Feng, L. Wang, Y. Huang The golden takin is an endangered species, listed as a First Grade Protected animal and found only in the Qinling Mountains in China. A great deal of research on the golden takin's living habitat, population size, and home range has been conducted. Here, we employed sequence analysis of the mitochondrial DNA control region to study the genetic diversity of the golden takin from three separate nature reserve parks in the Qinling Mountains. We also compared the results of our study with prev...
Source: Biochemical Systematics and Ecology - November 4, 2016 Category: Biochemistry Source Type: research

Leaf and stem galls of Schinus polygamus (Cav.) Cabr (Anacardiaceae): Anatomical and chemical implications
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Lubia M. Guedes, Narciso Aguilera, José Becerra, Víctor Hernández, Rosy M. dos Santos Isaias Galling insects commonly induce anatomical and metabolic changes in their host plant tissues, which is true for Schinus polygamus (Cav.) Cabr. (Anacardiaceae) in Chile. Currently, anatomical and chemical changes induced by galling insects in stems and leaves of S. polygamus were analyzed. Methanolic extracts of non-galled and galled tissues were analyzed by gas chromatography–mass spectrometry (GC-MS). Differ...
Source: Biochemical Systematics and Ecology - October 30, 2016 Category: Biochemistry Source Type: research

Chemical constituents from the fruits of Rhus typhina L. and their chemotaxonomic significance
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Da-Ren Qiu, Da-Cheng Wang, Sheng-Xiang Yang, Ya-Mei Zhang, Dong-Sheng Wei, Ming-Zhe Zhang, Jin-Zhu Sun, Jie Cong, Jie Guo, Shu-Liang He, Jian-Chun Qin This work describes the isolation and characterization of twenty-nine compounds from the fruits of Rhus typhina L., including eleven flavonoids (1–11), eleven phenols (12–22), two pentacyclic triterpenes (23–24), two organic acids (25–26), one lumichrome (27), one courmarin (28) and one pyrimidine (29) on the basis of their spectroscopic data. Compounds a...
Source: Biochemical Systematics and Ecology - October 30, 2016 Category: Biochemistry Source Type: research

Biosystematic study in the genus Cousinia Cass. (Asteraceae), section Cousinia
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Amir Abbas Minaeifar, Masoud Sheidai, Farideh Attar, Zahra Noormohammadi, Somayeh Ghasemzadeh-Baraki The genus Cousinia Cass. (Family Asteraceae) with about 600–700 species is distributed in central and western Asia. Iran has more than 200 Cousinia species that grow in mountainous areas. Cousinia is not monophyletic and with the genus Arctium, comprise the Arctium-Cousinia complex. The Cousinia species are distributed in 70 sections. The species relationship in the sect. Cousinia is not known, moreover, in few cases, int...
Source: Biochemical Systematics and Ecology - October 30, 2016 Category: Biochemistry Source Type: research

Transcriptional reprogramming of genes related to ascorbate and glutathione biosynthesis, turnover and translocation in aphid-challenged maize seedlings
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Hubert Sytykiewicz Aphids are pierce-sucking insects that severely affect redox homeostasis in the host tissues. Reduced ascorbate (AsA) and glutathione (GSH) serve as multifunctional molecules involved in constituting sophisticated plant defense responses to aphids' herbivory. This survey aimed to decipher transcriptional responses of forty-one genes associated with AsA and GSH biosynthesis, turnover and translocation in maize (Zea mays L.) seedlings infested with two hemipterans' species: bird cherry-oat aphid (Rhopalosiphum...
Source: Biochemical Systematics and Ecology - October 30, 2016 Category: Biochemistry Source Type: research

Characterization of the complete mitochondrial genome of Phodopus roborovskii (Rodentia: Cricetidae) and systematic implications for Cricetinae phylogenetics
This study reports its complete mitochondrial genome (mitogenome) for the first time. The complete sequence was 16,273 bp long, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and 1 major noncoding region. The base composition and codon usage were described. The putative origin of replication for the light strand (OL) of P. roborovskii was approximately 45 bp long and was highly conserved in the stem-loop and adjacent sequences, but the starting sequence of replication varied between genera among Rodentia. We analyzed the three domains of the D-loop region, and the results indicated that th...
Source: Biochemical Systematics and Ecology - October 30, 2016 Category: Biochemistry Source Type: research

Isoquinoline alkaloids from the leaves of Annona leptopetala (Annonaceae)
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Cristiane Maria Souza de Castro Rodrigues, Lívia Macedo Dutra, Andersson Barison, Emmanoel Vilaça Costa, Jackson Roberto Guedes da Silva Almeida The phytochemical investigation of the leaves of Annona leptopetala (R. E. Fr.) H. Rainer led to the characterization of tetrahydroprotoberberine corypalmine, and four aporphine (laurotetanine, anonaine, norannuradhapurine and nornuciferine) alkaloids. The structures were established after analysis of their NMR spectral data including 2D NMR experiments. This is the firs...
Source: Biochemical Systematics and Ecology - October 30, 2016 Category: Biochemistry Source Type: research

Phylogenetic relationships among species of Tamarix (Tamaricaceae) in China
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Likun Sun, Ruiqi Yang, Baogui Zhang, Gaosen Zhang, Xiukun Wu, Wei Zhang, Binlin Zhang, Tuo Chen, Guangxiu Liu Tamarix is a genus in family Tamaricaceae. The present study inferred phylogenetic trees for sixteen Tamarix species from China, whose status has remained unclear, using the nuclear internal transcribed spacer (ITS) region and four chloroplast DNA regions. Thirty ITS sequences from the GenBank database were added for expanded ITS analysis. The molecular phylogenies divide the Tamarix species into eight clades. Tamarix ...
Source: Biochemical Systematics and Ecology - October 30, 2016 Category: Biochemistry Source Type: research

Population genetic structure and demographic history of Medicago ruthenica (Fabaceae) on the Qinghai-Tibetan Plateau based on nuclear ITS and chloroplast markers
In this study, nuclear internal transcribed spacer (ITS) and chloroplast (psbA-trnH) DNA regions were used to evaluate the genetic diversity, structure and population dynamics of 12 wild M. ruthenica populations distributed on the QTP and Inner Mongolia. Although the chloroplast psbA-trnH region was more variable than nuclear ITS, both markers indicated high interpopulation genetic differentiation, with all populations clustered into two groups based on neighbor joining clustering. Mismatch distribution and neutrality tests revealed local or small-scale demographic expansion. As inferred by morphology and genetic structure...
Source: Biochemical Systematics and Ecology - October 15, 2016 Category: Biochemistry Source Type: research

Characterization and phylogenetic analysis of the complete mitogenome of Allactaga sibirica (Rodentia: Dipodidae)
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Li Ding, Guangjie Luo, Wenjia Li, Jicheng Liao Allactaga sibirica (Dipodidae) is widely distributed in the northwestern arid regions of China. The complete mitochondrial genome (mitogenome) of A. sibirica was 16,685 bp in length; included 13 protein-coding genes, 2 ribosome genes, 22 tRNA genes, and one control region; and had a structure that was typical of vertebrates. The base composition and codon usage of the mitogenome are described, and the structure of the non-coding sequence in the A. sibirica is reported for the...
Source: Biochemical Systematics and Ecology - October 15, 2016 Category: Biochemistry Source Type: research

Using the ITS2 sequence-structure as a DNA mini-barcode: A  case study in authenticating the traditional medicine“Fang Feng”
In this study, we selected 135 individuals that represented 97 species from 21 genera to explore the possibility of employing the short ITS2 fragment as a barcode to identify the traditional medicine “Fang Feng” using sequence and structure information. The results revealed that all of the ITS2 sequences had a common four-helix folding structure and some shared motifs. This conserved structure facilitated the alignment of ambiguous sequences from highly divergent taxa. The sequence-structure data yielded a maximum parsimony (MP) tree in which the species referred to as “Fang Feng” was successfully i...
Source: Biochemical Systematics and Ecology - October 15, 2016 Category: Biochemistry Source Type: research

Genetic relatedness and taxonomy in closely related species of Hedysarum (Fabaceae)
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Natalia S. Zvyagina, Olga V. Dorogina, Pilar Catalan A multidisciplinary study, engaging morphological, carpological and molecular data, has been performed to investigate the genetic relatedness and taxonomic boundaries of the close species Hedysarum gmelinii, H. setigerum and H. chaiyrakanicum (Fabaceae) with overlapped distribution areas in southern Siberia. The diagnostic features of these legume species are analyzed and discussed, including their macro- and micromorphological characteristics, seed coat ornamentation and in...
Source: Biochemical Systematics and Ecology - October 14, 2016 Category: Biochemistry Source Type: research

GC-MS analysis of the essential oils of Juniperus communis L.  berries growing wild in the Molise region: Seasonal variability and in vitro antifungal activity
We examined the berries in different phases of the biological cycle for 1 year (at six ripening stages). Hydrodistilled essential oils from the fresh berries were analyzed by gas chromatography–flame ionization detection (GC-FID), gas chromatography–mass spectrometry (GC-MS) and principal component analysis (PCA). A total of 90 components were detected, and remarkable qualitative and quantitative differences were observed in the chemical components during the ripening stages, from the green unripe berries to the bluish-black berries harvested at full maturity. The essential oils were an α-pinene (13.43&nd...
Source: Biochemical Systematics and Ecology - October 12, 2016 Category: Biochemistry Source Type: research

Transferability of microsatellite markers across six Dalbergia (Fabaceae) species and their characterization for Dalbergia miscolobium
In this study, 18 microsatellite markers previously developed and characterized for two Dalbergia species (Dalbergia nigra and Dalbergia monticola) were tested for six other species of the genus: Dalbergia decipularis, Dalbergia ecastaphyllum, Dalbergia elegans, Dalbergia frutescens, Dalbergia miscolobium, and Dalbergia sissoo. All species except D. sissoo are native to Brazil, and some have high-quality timber. Among the tested loci, 14 showed successful amplification in at least two species, and polymerase chain reaction (PCR) conditions were optimized for each of 8–12 transferred markers. Nine polymorphic loci wer...
Source: Biochemical Systematics and Ecology - October 1, 2016 Category: Biochemistry Source Type: research

Genetic diversity and population structure analysis of Qinghai-Tibetan plateau schizothoracine fish (Gymnocypris dobula) based on mtDNA D-loop sequences
In this study, genetic diversity and intraspecific population differentiation were examined by using mitochondrial DNA D-loop sequences in 97 individuals sampled from three localities (Pali, Lasa and Yanghu) in the Tibetan plateau. Two hundred and fourteen polymorphic sites and 50 haplotypes were defined among the three localities. Genetic diversity analysis showed that the highest genetic diversity level was found in Pali population. Phylogenetic relationships analysis results indicated that closer phylogenetic relationships were found between the Yanghu and Lasa populations. Genetic population differentiation analysis in...
Source: Biochemical Systematics and Ecology - September 18, 2016 Category: Biochemistry Source Type: research

Genetic diversity and structure among natural populations of Sindora glabra in Hainan Island, China as revealed by ISSR markers
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Jin-chang Yang, Qiong-Qiong Li, Niu Yu, Guang-tian Yin, Zhong-feng Wu, Rong-sheng Li, Wen-tao Zou Sindora glabra, one of the second-class national protective plants in China, has important ecological and commercial values. To understand the genetic diversity and structure of S. glabra, eleven natural populations from four areas of Hainan Island in China were analyzed by inter-simple sequence repeat (ISSR) markers. Thirteen primers were screened out and used to amplify 157 DNA samples. A total of 122 bands were obtained, among ...
Source: Biochemical Systematics and Ecology - September 15, 2016 Category: Biochemistry Source Type: research

Comparative studies on the life history characteristics of two Brachionus calyciflorus strains belonging to the same cryptic species
In this study, the phylogenetic relationships of Brachionus calyciflorus collecting from two climatic zones were reconstructed, and two clones of the same potential cryptic species were selected as representatives of two populations. The life history characteristics of the two B. calyciflorus strains were comparatively studied at four different temperatures (20 °C, 24 °C, 28 °C and 32 °C). The results showed that the responses of the life history traits of B. calyciflorus to temperature were strain-dependent. The Xishuangbanna strain is more suitable for growth at the higher temperature ...
Source: Biochemical Systematics and Ecology - September 15, 2016 Category: Biochemistry Source Type: research

Genetic diversity of Rheum tanguticum (Polygonaceae), an endangered species on Qinghai-Tibetan Plateau
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Ailan Wang, Weiwei Li Rheum tanguticum (Polygonaceae), a plant species used in both traditional Chinese and Tibetan medicine, has been listed as endangered species because its distribution in the Tibetan Plateau has rapidly decreased in recent years. We estimated the genetic diversity of R. tanguticum using the cpDNA trnL-F region for 95 individuals from nine populations. The results demonstrated high genetic diversity in this species (Ht = 0.632), primarily due to variation among populations (68.4%, GST = ...
Source: Biochemical Systematics and Ecology - September 15, 2016 Category: Biochemistry Source Type: research

Complete mitochondrial genome of Orthetrum dragonflies and molecular phylogeny of Odonata
We report here the complete mitogenome of O. chrysis, O. glaucum, O. sabina and O. testaceum and their phylogenetic relationships with other taxa of Libellulidae as well as Epiophlebiidae, Anisoptera and Zygoptera. The whole mitogenomes of these four species possessed 37 genes (13 protein-coding genes – PCGs, 2 rRNA and 22 tRNA genes) and a non-coding region. Molecular phylogeny based on 13 PCGs was concordant with 15 mitochondrial genes (13 PCGs and 2 rRNA genes). The Libellulidae (Anisoptera) was monophyletic with two lineages: (Orthetrum) + (Brachythemis&...
Source: Biochemical Systematics and Ecology - September 11, 2016 Category: Biochemistry Source Type: research

A multigene approach to determine the molecular phylogeography of Argiope mangal and Argiope dang (Araneae: Araneidae) and their genetic relationships with the Argiope aetherea species group
In this study for the first time, genetic data of A. mangal were reported for specimens collected from its type locality, Singapore. Four new distribution records were also reported: A. dang in Peninsular Malaysia and Cambodia; A. modesta in Bali, Indonesia; and A. jinghongensis in Peninsular Malaysia. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - September 11, 2016 Category: Biochemistry Source Type: research

Genders matters: Sexual differences in chemical signals of Liolaemus wiegmannii lizards (Iguania, Liolaemidae)
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Roberto García-Roa, Santiago Carreira, Pilar López, José Martín Males have traditionally been the focus of study when analyzing traits involved in communication. However, intraspecific interactions often imply both sexes as signals senders. Researches have shown the relevant role that chemical communication plays in lizards. However, the knowledge about the female scents is scarce. The aim of this paper is to characterize, by using gas chromatography-mass spectrometry (GC-MS), the lipophilic fractio...
Source: Biochemical Systematics and Ecology - September 10, 2016 Category: Biochemistry Source Type: research

The mitogenomes of three beetles (Coleoptera: Polyphaga: Cucujiformia): New gene rearrangement and phylogeny
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Xuejuan Li, Jing Ou, Zhaoming Wei, Yaxiao Li, Yingfang Tian The mitochondrial genome (mitogenome) has been extensively used for studying phylogenetic relationships at different taxonomic levels. Several molecular analyses have been performed, but the phylogenetic relationships among infraorders in Polyphaga have not been well resolved. In this work, three nearly complete mitogenomes of Coleoptera, Sitophilus oryzae, Oryzaephilus surinamensis and Callosobruchus chinensis, were determined. The O. surinamensis and S. oryzae mitog...
Source: Biochemical Systematics and Ecology - September 3, 2016 Category: Biochemistry Source Type: research

Studies on genetic diversity and phylogenetic relationships of limpograss (Hemarthria altissima) and related species based on combined chloroplast DNA intergenic spacer data
This study is to use three cpDNA intergenic spacers (trnL-F, trnC-ycf6 and psbC-trnS) to obtain phylogenetic information in 36 Hemarthria samples including four Hemarthria species: Hemarthria altissima (Poir.) Stapf et C. E. Hubb., Hemarthria compressa (L. f.) R. Br., Hemarthria uncinata R. Br., and Hemarthria japonica (Hack.) Roshev. Data analysis revealed that non-significant genetic diversity existed in our samples, which was implied by nucleotide sequences information and the results of haplotypic and nucleotide diversity. The results of phylogenetic trees based on maximum likelihood (ML) and Bayesian inference (BI) re...
Source: Biochemical Systematics and Ecology - September 1, 2016 Category: Biochemistry Source Type: research

Complete mitochondrial genome of Pycnonotus xanthorrhous (Passeriformes, Pycnonotidae) and phylogenetic consideration
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Longying Wen, Fei Liao The complete mitochondrial genome (mitogenome) of Pycnonotus xanthorrhous was sequenced via next generation sequencing. The full length of the circular genome is 16,952 bp. It consists of 37 typical animal mitochondrial genes including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) and 2 ribosomal RNA (rRNA) genes. P. xanthorrhous also contains one control region (CR) and one pseudo-control region, and shares the identical gene arrangements with sequenced Pycnonotus spp. which differs from the ty...
Source: Biochemical Systematics and Ecology - September 1, 2016 Category: Biochemistry Source Type: research

Fragmented habitat drives significant genetic divergence in the Chinese endemic plant, Urophysa henryi (Ranuculaceae)
In this study, ISSR markers were used to assess the genetic diversity and population structure throughout its entire distribution areas. Twelve primers revealed high genetic diversity at the species level (PPB = 95.6%; H = 0.3441; I = 0.5111), as well as high level of genetic differentiation (F ST = 0.659, p < 0.001; G ST = 0.677) and restricted gene flow (Nm = 0.239) among populations. According to the UPGMA and PCoA analysis, the 9 populations were clustered into three main groups, which were roughly in accordance with their geographical regions...
Source: Biochemical Systematics and Ecology - September 1, 2016 Category: Biochemistry Source Type: research

Genetic diversity of Meconopsis integrifolia (Maxim.) Franch. In the East Himalaya –Hengduan Mountains inferred from fluorescent amplified fragment length polymorphism analysis
In this study, the genetic diversity and structure of M. integrifolia, represented by 183 individuals from 10 populations, were studied using 6 pairs of fluorescent Amplified Fragment Length Polymorphisms primers. Our results showed a relatively high genetic variation at the species level (PPB = 82.0%, H E  = 0.2356 and I = 0.3695). In contrast, population diversity was extremely low, as measured by Nei's genetic diversity index and Shannon's diversity index (H E  = 0.0317, I = 0.0480). Analyses of molecular variance (AMOVA) showed that among and within populations, the gen...
Source: Biochemical Systematics and Ecology - August 31, 2016 Category: Biochemistry Source Type: research

Mitochondrial genetic diversity of Rhinogobius giurinus (Teleostei: Gobiidae) in East Asia
In this study, R. giurinus from Japan, Taiwan and mainland China were used to evaluate the population genetic structure using mitochondrial DNA cytochrome b gene sequences (1122 bp). In total, 123 sequences were analyzed from seventeen populations. All 44 mtDNA haplotypes were identified as belonging to ten haplogroups, and four haplogroups were only distributed in the upstream of the Yangtze River and Hainan Island. The phylogeny and geography did not have a significant relationship. Our results (1) found that the mtDNA genetic diversity of this species was less than that of the freshwater goby; (2) showed that the lack o...
Source: Biochemical Systematics and Ecology - August 28, 2016 Category: Biochemistry Source Type: research

Low levels of genetic diversity in Crocodylus acutus in Oaxaca and Guerrero, Mexico, and molecular-morphological evidence of the presence of C. moreletii
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Samuel S. Serrano-Gómez, Luis M. Guevara-Chumacero, Irene D.L.A. Barriga-Sosa, Raúl Ullóa-Arvízu, Sofía González-Guzmán, Carlos G. Vázquez-Peláez The genetic variation in C. acutus from the Pacific coast of Mexico was investigated using mitochondrial DNA data. Overall, the localities sampled included three in the State of Guerrero and three in the State of Oaxaca, Mexico. We inferred phylogenetic and genealogical relationships, and genetic diversity was determined,...
Source: Biochemical Systematics and Ecology - August 26, 2016 Category: Biochemistry Source Type: research

Sequencing and phylogenetic analysis of the chloroplast genome of Pseudosasa japonica f. Akebonosuji
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Mingbing Zhou, Miaomiao An, Xiangwan Xia, Haiyun Yang, Minmin Cheng, Kaili Wang, Wei Fang Pseudosasa japonica f. Akebonosuji H. Okamura is a bamboo species with variable leaf colors, including albino, green, and green-white stripes. To determine whether variation in leaf color is due to mutations in the chloroplast genome, we sequenced the chloroplast genomes of green and albino leaves of P. japonica f. Akebonosuji. The results indicated that the chloroplast genome included 86 protein-coding genes, seven ribosomal RNA genes, a...
Source: Biochemical Systematics and Ecology - August 26, 2016 Category: Biochemistry Source Type: research

Molecular phylogeny and systematics of the Barbinae (Teleostei: Cyprinidae) in China inferred from mitochondrial DNA sequences
In this study, partial sequences of four mitochondrial genes (cyt b, COI, ND4 and 16S rRNA) from 75 Barbinae species and 38 outgroup species were used to investigate the taxonomy and phylogeny within the Barbinae in China. The monophyly of Neolissochilus, Sikukia and Tor are not supported. Neolissochilus benasi might represent a new genus, and Tor hemispinus and Tor qiaojiensis should be moved into Neolissochilus. Sikukia flavicaudata is not Sikukia species. Puntius paucimaculatus might be a synonym of Puntius semifasciolatus. Puntius semifasciolatus does not belong to Puntius. Onychostoma barbatum might consist of more th...
Source: Biochemical Systematics and Ecology - August 24, 2016 Category: Biochemistry Source Type: research

Hematological parameters of three freshwater stingray species (Chondrichthyes: Potamotrygonidae) in the middle Rio Negro, Amazonas state
In conclusion, the development stage is an important factor for differentiating hematological properties in the cururu stingray, while this has not been observed in P. motoro and P. aiereba stingrays. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - August 24, 2016 Category: Biochemistry Source Type: research

Assessment of genetic diversity and population differentiation of Achyranthes bidentata (Amaranthaceae) in Dao Di and its surrounding region based on microsatellite markers
This study thus provides an important case for over-collecting within limited ranges in affecting population diversity and bioclimate variables for different producing area in driving population differentiation. (Source: Biochemical Systematics and Ecology)
Source: Biochemical Systematics and Ecology - August 22, 2016 Category: Biochemistry Source Type: research

Phylogenetic relationships and genetic diversity of badgers from the Korean Peninsula: Implications for the taxonomic status of the Korean badger
Publication date: December 2016 Source:Biochemical Systematics and Ecology, Volume 69 Author(s): Mu-Yeong Lee, Seon-Mi Lee, Eui-Geun Song, Jung Hwa An, Inna Voloshina, Jong Royl Chong, Warren E. Johnson, Mi-Sook Min, Hang Lee Accurate taxonomic classification of wildlife species is crucial for guiding biological research and for developing effective management and conservation programs. The taxonomic status of Eurasian badgers from South Korea remains poorly resolved. Here we assessed the phylogenetic relationships and genetic variation of Eurasia badgers using partial mitochondrial fragments to elucidate the evolutionary...
Source: Biochemical Systematics and Ecology - August 22, 2016 Category: Biochemistry Source Type: research