Biochemical approaches to assess the impact of post-translational modifications on pathogenic tau conformations using recombinant protein
Biochem Soc Trans. 2024 Feb 13:BST20230596. doi: 10.1042/BST20230596. Online ahead of print.ABSTRACTTau protein is associated with many neurodegenerative disorders known as tauopathies. Aggregates of tau are thought of as a main contributor to neurodegeneration in these diseases. Increasingly, evidence points to earlier, soluble conformations of abnormally modified monomers and multimeric tau as toxic forms of tau. The biological processes driving tau from physiological species to pathogenic conformations remain poorly understood, but certain avenues are currently under investigation including the functional consequences o...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Mohammed M Alhadidy Nicholas M Kanaan Source Type: research
Backbone interactions and secondary structures in phase separation of disordered proteins
Biochem Soc Trans. 2024 Feb 13:BST20230618. doi: 10.1042/BST20230618. Online ahead of print.ABSTRACTIntrinsically disordered proteins (IDPs) are one of the major drivers behind the formation and characteristics of biomolecular condensates. Due to their inherent flexibility, the backbones of IDPs are significantly exposed, rendering them highly influential and susceptible to biomolecular phase separation. In densely packed condensates, exposed backbones have a heightened capacity to interact with neighboring protein chains, which might lead to strong coupling between the secondary structures and phase separation and further...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Shanlong Li Yumeng Zhang Jianhan Chen Source Type: research
The molecular machinery of meiotic recombination
Biochem Soc Trans. 2024 Feb 13:BST20230712. doi: 10.1042/BST20230712. Online ahead of print.ABSTRACTMeiotic recombination, a cornerstone of eukaryotic diversity and individual genetic identity, is essential for the creation of physical linkages between homologous chromosomes, facilitating their faithful segregation during meiosis I. This process requires that germ cells generate controlled DNA lesions within their own genome that are subsequently repaired in a specialised manner. Repair of these DNA breaks involves the modulation of existing homologous recombination repair pathways to generate crossovers between homologous...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Linda Chen John R Weir Source Type: research
S-acylation of Ca2+ transport proteins: molecular basis and functional consequences
Biochem Soc Trans. 2024 Feb 13:BST20230818. doi: 10.1042/BST20230818. Online ahead of print.ABSTRACTCalcium (Ca2+) regulates a multitude of cellular processes during fertilization and throughout adult life by acting as an intracellular messenger to control effector functions in excitable and non-excitable cells. Changes in intracellular Ca2+ levels are driven by the co-ordinated action of Ca2+ channels, pumps, and exchangers, and the resulting signals are shaped and decoded by Ca2+-binding proteins to drive rapid and long-term cellular processes ranging from neurotransmission and cardiac contraction to gene transcription a...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Rapha ël Néré Sana Kouba Amado Carreras-Sureda Nicolas Demaurex Source Type: research
DDX41: exploring the roles of a versatile helicase
Biochem Soc Trans. 2024 Feb 13:BST20230725. doi: 10.1042/BST20230725. Online ahead of print.ABSTRACTDDX41 is a DEAD-box helicase and is conserved across species. Mutations in DDX41 have been associated with myeloid neoplasms, including myelodysplastic syndrome and acute myeloid leukemia. Though its pathogenesis is not completely known, DDX41 has been shown to have many cellular roles, including in pre-mRNA splicing, innate immune sensing, ribosome biogenesis, translational regulation, and R-loop metabolism. In this review, we will summarize the latest understandings regarding the various roles of DDX41, as well as highligh...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Lacey Winstone Yohan Jung Yuliang Wu Source Type: research
Biochemical approaches to assess the impact of post-translational modifications on pathogenic tau conformations using recombinant protein
Biochem Soc Trans. 2024 Feb 13:BST20230596. doi: 10.1042/BST20230596. Online ahead of print.ABSTRACTTau protein is associated with many neurodegenerative disorders known as tauopathies. Aggregates of tau are thought of as a main contributor to neurodegeneration in these diseases. Increasingly, evidence points to earlier, soluble conformations of abnormally modified monomers and multimeric tau as toxic forms of tau. The biological processes driving tau from physiological species to pathogenic conformations remain poorly understood, but certain avenues are currently under investigation including the functional consequences o...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Mohammed M Alhadidy Nicholas M Kanaan Source Type: research
Backbone interactions and secondary structures in phase separation of disordered proteins
Biochem Soc Trans. 2024 Feb 13:BST20230618. doi: 10.1042/BST20230618. Online ahead of print.ABSTRACTIntrinsically disordered proteins (IDPs) are one of the major drivers behind the formation and characteristics of biomolecular condensates. Due to their inherent flexibility, the backbones of IDPs are significantly exposed, rendering them highly influential and susceptible to biomolecular phase separation. In densely packed condensates, exposed backbones have a heightened capacity to interact with neighboring protein chains, which might lead to strong coupling between the secondary structures and phase separation and further...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Shanlong Li Yumeng Zhang Jianhan Chen Source Type: research
The molecular machinery of meiotic recombination
Biochem Soc Trans. 2024 Feb 13:BST20230712. doi: 10.1042/BST20230712. Online ahead of print.ABSTRACTMeiotic recombination, a cornerstone of eukaryotic diversity and individual genetic identity, is essential for the creation of physical linkages between homologous chromosomes, facilitating their faithful segregation during meiosis I. This process requires that germ cells generate controlled DNA lesions within their own genome that are subsequently repaired in a specialised manner. Repair of these DNA breaks involves the modulation of existing homologous recombination repair pathways to generate crossovers between homologous...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Linda Chen John R Weir Source Type: research
S-acylation of Ca2+ transport proteins: molecular basis and functional consequences
Biochem Soc Trans. 2024 Feb 13:BST20230818. doi: 10.1042/BST20230818. Online ahead of print.ABSTRACTCalcium (Ca2+) regulates a multitude of cellular processes during fertilization and throughout adult life by acting as an intracellular messenger to control effector functions in excitable and non-excitable cells. Changes in intracellular Ca2+ levels are driven by the co-ordinated action of Ca2+ channels, pumps, and exchangers, and the resulting signals are shaped and decoded by Ca2+-binding proteins to drive rapid and long-term cellular processes ranging from neurotransmission and cardiac contraction to gene transcription a...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Rapha ël Néré Sana Kouba Amado Carreras-Sureda Nicolas Demaurex Source Type: research
DDX41: exploring the roles of a versatile helicase
Biochem Soc Trans. 2024 Feb 13:BST20230725. doi: 10.1042/BST20230725. Online ahead of print.ABSTRACTDDX41 is a DEAD-box helicase and is conserved across species. Mutations in DDX41 have been associated with myeloid neoplasms, including myelodysplastic syndrome and acute myeloid leukemia. Though its pathogenesis is not completely known, DDX41 has been shown to have many cellular roles, including in pre-mRNA splicing, innate immune sensing, ribosome biogenesis, translational regulation, and R-loop metabolism. In this review, we will summarize the latest understandings regarding the various roles of DDX41, as well as highligh...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Lacey Winstone Yohan Jung Yuliang Wu Source Type: research
Biochemical approaches to assess the impact of post-translational modifications on pathogenic tau conformations using recombinant protein
Biochem Soc Trans. 2024 Feb 13:BST20230596. doi: 10.1042/BST20230596. Online ahead of print.ABSTRACTTau protein is associated with many neurodegenerative disorders known as tauopathies. Aggregates of tau are thought of as a main contributor to neurodegeneration in these diseases. Increasingly, evidence points to earlier, soluble conformations of abnormally modified monomers and multimeric tau as toxic forms of tau. The biological processes driving tau from physiological species to pathogenic conformations remain poorly understood, but certain avenues are currently under investigation including the functional consequences o...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Mohammed M Alhadidy Nicholas M Kanaan Source Type: research
Backbone interactions and secondary structures in phase separation of disordered proteins
Biochem Soc Trans. 2024 Feb 13:BST20230618. doi: 10.1042/BST20230618. Online ahead of print.ABSTRACTIntrinsically disordered proteins (IDPs) are one of the major drivers behind the formation and characteristics of biomolecular condensates. Due to their inherent flexibility, the backbones of IDPs are significantly exposed, rendering them highly influential and susceptible to biomolecular phase separation. In densely packed condensates, exposed backbones have a heightened capacity to interact with neighboring protein chains, which might lead to strong coupling between the secondary structures and phase separation and further...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Shanlong Li Yumeng Zhang Jianhan Chen Source Type: research
The molecular machinery of meiotic recombination
Biochem Soc Trans. 2024 Feb 13:BST20230712. doi: 10.1042/BST20230712. Online ahead of print.ABSTRACTMeiotic recombination, a cornerstone of eukaryotic diversity and individual genetic identity, is essential for the creation of physical linkages between homologous chromosomes, facilitating their faithful segregation during meiosis I. This process requires that germ cells generate controlled DNA lesions within their own genome that are subsequently repaired in a specialised manner. Repair of these DNA breaks involves the modulation of existing homologous recombination repair pathways to generate crossovers between homologous...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Linda Chen John R Weir Source Type: research
S-acylation of Ca2+ transport proteins: molecular basis and functional consequences
Biochem Soc Trans. 2024 Feb 13:BST20230818. doi: 10.1042/BST20230818. Online ahead of print.ABSTRACTCalcium (Ca2+) regulates a multitude of cellular processes during fertilization and throughout adult life by acting as an intracellular messenger to control effector functions in excitable and non-excitable cells. Changes in intracellular Ca2+ levels are driven by the co-ordinated action of Ca2+ channels, pumps, and exchangers, and the resulting signals are shaped and decoded by Ca2+-binding proteins to drive rapid and long-term cellular processes ranging from neurotransmission and cardiac contraction to gene transcription a...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Rapha ël Néré Sana Kouba Amado Carreras-Sureda Nicolas Demaurex Source Type: research
DDX41: exploring the roles of a versatile helicase
Biochem Soc Trans. 2024 Feb 13:BST20230725. doi: 10.1042/BST20230725. Online ahead of print.ABSTRACTDDX41 is a DEAD-box helicase and is conserved across species. Mutations in DDX41 have been associated with myeloid neoplasms, including myelodysplastic syndrome and acute myeloid leukemia. Though its pathogenesis is not completely known, DDX41 has been shown to have many cellular roles, including in pre-mRNA splicing, innate immune sensing, ribosome biogenesis, translational regulation, and R-loop metabolism. In this review, we will summarize the latest understandings regarding the various roles of DDX41, as well as highligh...
Source: Biochemical Society Transactions - February 13, 2024 Category: Biochemistry Authors: Lacey Winstone Yohan Jung Yuliang Wu Source Type: research
