Clinical characteristics of outpatients with influenza-B-associated pneumonia and molecular evolution of influenza B virus in Beijing, China, during the 2021 –2022 influenza season
AbstractWe analyzed the clinical characteristics of outpatients with influenza-B-associated pneumonia during the 2021 –2022 influenza season and analyzed the molecular epidemiology and evolution of influenza B virus. The presence of influenza B virus was confirmed by reverse transcription polymerase chain reaction (RT-PCR). Electronic medical records were used to collect and analyze data of outpatients. The HA an d NA genes were phylogenetically analyzed using ClustalW 2.10 and MEGA 11.0. Out of 1569 outpatients who tested positive for influenza B virus, 11.7% (184/1569) developed pneumonia, and of these, 19.0% (35/184) ...
Source: Archives of Virology - January 18, 2024 Category: Virology Source Type: research
The impact of PA/I38 substitutions and PA polymorphisms on the susceptibility of zoonotic influenza A viruses to baloxavir
AbstractGenetic reassortment of avian, swine, and human influenza A viruses (IAVs) poses potential pandemic risks. Surveillance is important for influenza pandemic preparedness, but the susceptibility of zoonotic IAVs to the cap-dependent endonuclease inhibitor baloxavir acid (BXA) has not been thoroughly researched. Although an amino acid substitution at position 38 in the polymerase acidic protein (PA/I38) in seasonal IAVs reduces BXA susceptibility, PA polymorphisms at position 38 are rarely seen in zoonotic IAVs. Here, we examined the impact of PA/I38 substitutions on the BXA susceptibility of recombinant A(H5N1) virus...
Source: Archives of Virology - January 12, 2024 Category: Virology Source Type: research
Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain
AbstractSenecavirus A (SVA) is an emerging virus that causes vesicular disease in pigs. Construction of a full-length SVA cDNA clone is crucial for understanding its replication and pathogenesis. Here, we successfully constructed a CMV-promoter-driven infectious cDNA clone of the SVA isolate SVA/GX/CH/2018, which we named rSVA GX01. Sequence comparison between the pSVA GX01 and the parental isolate (SVA/GX/CH/2018) revealed three single-nucleotide differences. Four-week-old piglets were experimentally infected with either the parental virus or the cloned virus. The results showed that the cloned rSVA GX01 displayed weak pa...
Source: Archives of Virology - January 12, 2024 Category: Virology Source Type: research
Identification of pigeon mitochondrial antiviral signaling protein (MAVS) and its role in antiviral innate immunity
This study not only sheds light on the function of piMAVS in innate immunity bu t also contributes to a more comprehensive understanding of the innate immunity system in poultry. Our data also provide unique insights into the differences in innate immunity between poultry and mammal. (Source: Archives of Virology)
Source: Archives of Virology - January 12, 2024 Category: Virology Source Type: research
Complete nucleotide sequence of chrysanthemum virus D, a polero-like virus
AbstractA putative new polerovirus, named “chrysanthemum virus D” (ChVD), was detected in aChrysanthemum morifolium plant in South Korea. The virus was identified by high-throughput sequencing and confirmed by reverse transcription polymerase chain reaction. The entire ChVD genome is composed of 5,963 nucleotides and contains seven open reading frames (ORF0-5 and ORF3a), which are arranged similarly to those of other poleroviruses. These ORFs encode the putative proteins P0-5 and P3a, respectively. Pairwise amino acid sequence comparisons showed that the ChVD P0-5 and P3a proteins have 30.45 –75% sequence identity to...
Source: Archives of Virology - January 12, 2024 Category: Virology Source Type: research
Complete sequence and genome characterization of miscanthus virus M, a new betaflexivirus from Miscanthus sp.
AbstractA novel betaflexivirus, tentatively named “miscanthus virus M” (MiVM), was isolated fromMiscanthus sp. The complete genome of MiVM is 7,388 nt in length (excluding the poly(A) tail). It contains five open reading frames and has a genome organization similar to those of members of the familiesAlphaflexiviridae andBetaflexiviridae (subfamilyQuinvirinae). The amino acid sequences of both the replicase and coat protein shared less than 45% identity with the corresponding sequences of members of either family. Phylogenetic analysis confirmed that MiVM belongs to the familyBetaflexiviridae and subfamilyQuinvirinae bu...
Source: Archives of Virology - January 12, 2024 Category: Virology Source Type: research
Complete genome sequence of a new putative member of the genus Pelarspovirus that infects Quercus aliena Blume in South Korea
AbstractThe complete nucleotide sequence of a newly discovered virus infectingQuercus aliena Blume, tentatively named “quercus leafroll virus” (QLRV), was determined through high-throughput and Sanger sequencing. The sequence comprises 3,940 nucleotides, has five open reading frames, and has a typical pelarspovirus genome organization, with neither 3' polyadenylation nor a 5' cap. The proteins encoded by QLRV s hare 17.9 to 44.2% amino acid sequence identity with known pelarspovirus proteins. The highest amino acid sequence identity values for the RNA-dependent RNA polymerase (RdRp) and coat protein were 67.5% and 55.2...
Source: Archives of Virology - January 11, 2024 Category: Virology Source Type: research
Construction of and evaluation of the immune response to two recombinant pseudorabies viruses expressing the B119L and EP364R proteins of African swine fever virus
In this study, two recombinant pseudorabies virus (PRV) strains, rGXGG-2016- ΔgI/ΔgE-EP364R and rGXGG-2016-ΔgI/ΔgE-B119L, expressing the EP364R and B119L protein, respectively, of ASFV, were constructed by homologous recombination technology. Western blotting and immunofluorescence analysis showed that these foreign proteins were expressed in cells infected with the reco mbinant strains. The strains showed good genetic stability and proliferative characteristics for 20 passages in BHK-21 cells. Both of these strains were immunogenic in mice, inducing the production of specific antibodies against the expressed ASFV prot...
Source: Archives of Virology - January 9, 2024 Category: Virology Source Type: research
Comparison of dye-based and probe-based RT-LAMP in detection of canine astrovirus
In this study, two real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays with high sensitivity, rapidity, and reliability were developed using fluorescence dye and FRET-based assimilating probes for real-time detection of CaAstV. These assays specifically amplified the ORF2 gene of CaAstV and did not amplify any sequences from canine enterovirus. The limit of detection (LOD) of both the probe-based and dye-based RT-LAMPs was 100 copies/ μL. Fluorescence signals were generated within 30 min for the lowest concentration of a standard RNA sample, which was significantly faster than that achi...
Source: Archives of Virology - January 9, 2024 Category: Virology Source Type: research
Genome sequence characterization of the unique recombinant vaccine-like lumpy skin disease virus strain Kurgan/2018
AbstractIn 2018, the molecular epidemiology of lumpy skin disease in Russia was characterized by a surge in novel recombinant vaccine-like strains causing outbreaks along the southern border, spreading in an easterly direction. Currently, five distinct novel recombinant vaccine-like lineages have been described, designated as clusters 2.1 to 2.5. Based on the complete genome sequence analysis of the causative lumpy skin disease virus (Kurgan/Russia/2018), obtained from an eponymous outbreak, the genome was shown to be composed of a Neethling vaccine strain virus as the dominant parental strain and KSGPO vaccine virus as it...
Source: Archives of Virology - January 9, 2024 Category: Virology Source Type: research
The unfolded protein response pathway as a possible link in the pathogenesis of COVID-19 and sepsis
This study provides a novel molecular perspective on the pathogenesis of sepsis and COVID-19 at the single-cell level and suggests that these two diseases may share a common mechanism. (Source: Archives of Virology)
Source: Archives of Virology - January 8, 2024 Category: Virology Source Type: research
Genomic variation in pepper vein yellows viruses in Australia, including a new putative variant, PeVYV-10
AbstractSince the first identification and full sequence of the polerovirus pepper vein yellows virus in Australia in 2016, virus surveys of crops and weeds have sporadically identified PeVYV in different hosts and locations. Genomic comparisons of 14 PeVYV-like isolates using RT-PCR products spanning the 3 ’ end of the RdRp region (ORF 2), the intergenic region, ORF 3a, ORF 4, and ORF 3 (1388 nt) showed that four of the PeVYV isolates might be a new variant or PeVYV-like virus. From six PeVYV-positive plants, eight PeVYV-like sequences were obtained by high-throughput sequencing, as two hosts, 5352 and 5634, contained t...
Source: Archives of Virology - January 5, 2024 Category: Virology Source Type: research
Complete genome sequence of a novel robigovirus infecting Mentha arvensis
AbstractThe complete genomic sequence of a novel robigovirus, provisionally named “Mentha arvensis robigovirus 1” (MARV1), was determined by combining next-generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR), and rapid amplification of cDNA ends (RACE) PCR. The complete genomic sequence of this new virus is 7617 nucleotides in length, excludi ng the 3′ poly(A) tail. The MARV1 genome encodes a putative replicase, “triple gene block” proteins, and a coat protein. Phylogenetic analysis demonstrated that MARV1 is a member of the genusRobigovirus, with closest relationships to African...
Source: Archives of Virology - January 5, 2024 Category: Virology Source Type: research
Pentavalent and poliomyelitis vaccines: a review of the vaccination coverage in Brazilian children in the last 10 years
AbstractThe pentavalent and poliomyelitis vaccines are provided to children through the National Immunization Program in Brazil. For the last few years, this program has been responsible for eradication of various diseases in the country. To describe the vaccination coverage of the pentavalent vaccine and the polio vaccine in Brazil from 2013 to 2022. This is a retrospective and descriptive study with a time series component in which we analyzed vaccination coverage rates of pentavalent and poliomyelitis in the national vaccination program of Brazil from 2013 to 2022. The collected data were arranged in spreadsheets, analy...
Source: Archives of Virology - January 4, 2024 Category: Virology Source Type: research
Using molecular methods to delineate norovirus outbreaks: a systematic review
This study was conducted to estimate the threshold value of genomic variations among related strains within norovirus outbreaks. We carried out a literature search in the PubMed and Web of Science databases. SNP rates were defined as the number of SNPs/sequence length (bp) × 100%. The Mann-Whitney U-test was used in comparisons of the distribution of SNP rates for different sequence regions, genogroups (GI and GII), transmission routes, and sequencing methods. A total of 25 articles reporting on 108 norovirus outbreaks were included. In 99.1% of the outbreaks, the SN P rates were below 0.50%, and in 89.8%, the SNP rates w...
Source: Archives of Virology - January 3, 2024 Category: Virology Source Type: research
