Development and validation of a human bronchial epithelial spheroid model to study respiratory toxicity in vitro
Arch Toxicol. 2023 Dec 26. doi: 10.1007/s00204-023-03619-9. Online ahead of print.ABSTRACTThe use of laboratory animals in research has been extensively criticized. While most of the critique has been centered around the ethical aspect, also the economic and scientific aspects have been frequently mentioned as points of concern. As a result, the use of alternative methods has gradually become more enticing. The most used alternatives to laboratory animals are the 2D monolayer cell cultures. However, the limited translatability of these monolayer cell cultures to in vivo has led to the development of 3D cell cultures that a...
Source: Archives of Toxicology - December 26, 2023 Category: Toxicology Authors: Thomas Celis Dominique M A Bullens Peter H M Hoet Manosij Ghosh Source Type: research

Hazard characterization of Alternaria toxins to identify data gaps and improve risk assessment for human health
Arch Toxicol. 2023 Dec 26. doi: 10.1007/s00204-023-03636-8. Online ahead of print.ABSTRACTFungi of the genus Alternaria are ubiquitous plant pathogens and saprophytes which are able to grow under varying temperature and moisture conditions as well as on a large range of substrates. A spectrum of structurally diverse secondary metabolites with toxic potential has been identified, but occurrence and relative proportion of the different metabolites in complex mixtures depend on strain, substrate, and growth conditions. This review compiles the available knowledge on hazard identification and characterization of Alternaria tox...
Source: Archives of Toxicology - December 26, 2023 Category: Toxicology Authors: Henriqueta Louro Ariane Vettorazzi Adela L ópez de Cerain Anastasia Spyropoulou Anita Solhaug Anne Straumfors Anne-Cathrin Behr Birgit Mertens Bojana Žegura Christiane Kruse F æste Dieynaba Ndiaye Eliana Spilioti Elisabeth Varga Estelle Dubreil Eszter Source Type: research

Review on novel toxicological effects and personalized health hazard in workers exposed to low doses of benzene
Arch Toxicol. 2023 Dec 24. doi: 10.1007/s00204-023-03650-w. Online ahead of print.ABSTRACTSeveral recent reports indicate health hazards for workers with below occupational limit exposure to benzene (BZ). Our updated review indicates that such low exposures induced traditional as well as novel toxicity/genotoxicity, e.g., increased mitochondria copy numbers, prolongation of telomeres, impairment of DNA damage repair response (DDRR), perturbations of expression in non-coding RNAs, and epigenetic changes. These abnormalities were associated with alterations of gene expression and cellular signaling pathways which affected he...
Source: Archives of Toxicology - December 24, 2023 Category: Toxicology Authors: Tongshuai Wang Yiyi Cao Zhaolin Xia David C Christiani William W Au Source Type: research

Review on novel toxicological effects and personalized health hazard in workers exposed to low doses of benzene
Arch Toxicol. 2023 Dec 24. doi: 10.1007/s00204-023-03650-w. Online ahead of print.ABSTRACTSeveral recent reports indicate health hazards for workers with below occupational limit exposure to benzene (BZ). Our updated review indicates that such low exposures induced traditional as well as novel toxicity/genotoxicity, e.g., increased mitochondria copy numbers, prolongation of telomeres, impairment of DNA damage repair response (DDRR), perturbations of expression in non-coding RNAs, and epigenetic changes. These abnormalities were associated with alterations of gene expression and cellular signaling pathways which affected he...
Source: Archives of Toxicology - December 24, 2023 Category: Toxicology Authors: Tongshuai Wang Yiyi Cao Zhaolin Xia David C Christiani William W Au Source Type: research

Analysis of vascular disruption in zebrafish embryos as an endpoint to predict developmental toxicity
Arch Toxicol. 2023 Dec 21. doi: 10.1007/s00204-023-03633-x. Online ahead of print.ABSTRACTInhibition of angiogenesis is an important mode of action for the teratogenic effect of chemicals and drugs. There is a gap in the availability of simple, experimental screening models for the detection of angiogenesis inhibition. The zebrafish embryo represents an alternative test system which offers the complexity of developmental differentiation of an entire organism while allowing for small-scale and high-throughput screening. Here we present a novel automated imaging-based method to detect the inhibition of angiogenesis in early ...
Source: Archives of Toxicology - December 22, 2023 Category: Toxicology Authors: Julia N öth Wibke Busch Tamara Tal Chih Lai Akhil Ambekar Tobias R Kie ßling Stefan Scholz Source Type: research

Analysis of vascular disruption in zebrafish embryos as an endpoint to predict developmental toxicity
Arch Toxicol. 2023 Dec 21. doi: 10.1007/s00204-023-03633-x. Online ahead of print.ABSTRACTInhibition of angiogenesis is an important mode of action for the teratogenic effect of chemicals and drugs. There is a gap in the availability of simple, experimental screening models for the detection of angiogenesis inhibition. The zebrafish embryo represents an alternative test system which offers the complexity of developmental differentiation of an entire organism while allowing for small-scale and high-throughput screening. Here we present a novel automated imaging-based method to detect the inhibition of angiogenesis in early ...
Source: Archives of Toxicology - December 22, 2023 Category: Toxicology Authors: Julia N öth Wibke Busch Tamara Tal Chih Lai Akhil Ambekar Tobias R Kie ßling Stefan Scholz Source Type: research

Analysis of vascular disruption in zebrafish embryos as an endpoint to predict developmental toxicity
Arch Toxicol. 2023 Dec 21. doi: 10.1007/s00204-023-03633-x. Online ahead of print.ABSTRACTInhibition of angiogenesis is an important mode of action for the teratogenic effect of chemicals and drugs. There is a gap in the availability of simple, experimental screening models for the detection of angiogenesis inhibition. The zebrafish embryo represents an alternative test system which offers the complexity of developmental differentiation of an entire organism while allowing for small-scale and high-throughput screening. Here we present a novel automated imaging-based method to detect the inhibition of angiogenesis in early ...
Source: Archives of Toxicology - December 22, 2023 Category: Toxicology Authors: Julia N öth Wibke Busch Tamara Tal Chih Lai Akhil Ambekar Tobias R Kie ßling Stefan Scholz Source Type: research

Physiologically-based pharmacokinetic model for evaluating gender-specific exposures of N-nitrosodimethylamine (NDMA)
Arch Toxicol. 2023 Dec 21. doi: 10.1007/s00204-023-03652-8. Online ahead of print.ABSTRACTN-nitrosodimethylamine (NDMA) is classified as a human carcinogen and could be produced by both natural and industrial processes. Although its toxicity and histopathology have been well-studied in animal species, there is insufficient data on the blood and tissue exposures that can be correlated with the toxicity of NDMA. The purpose of this study was to evaluate gender-specific pharmacokinetics/toxicokinetics (PKs/TKs), tissue distribution, and excretion after the oral administration of three different doses of NDMA in rats using a p...
Source: Archives of Toxicology - December 21, 2023 Category: Toxicology Authors: Dong Wook Kang Ju Hee Kim Go-Wun Choi Seok-Jin Cho Hea-Young Cho Source Type: research

Modeling the impact of heat stress on the toxicokinetics of toluene and acetone
Arch Toxicol. 2023 Dec 21. doi: 10.1007/s00204-023-03646-6. Online ahead of print.ABSTRACTMany workers can be exposed simultaneously to heat and volatile chemicals. In a controlled human exposure study, it was observed that an increase in ambient temperature was associated with increased blood concentrations for acetone and toluene. Based on the expected changes in physiological parameters that occur with an increase in ambient temperature, we aimed to develop a PBPK model for acetone and toluene that could account for the impact of temperature on the kinetics of these solvents. Changes in temperature-dependent physiologic...
Source: Archives of Toxicology - December 21, 2023 Category: Toxicology Authors: Axelle Marchand Jessie M énard Pierre Brochu Sami Haddad Source Type: research

Impact of perfluoroalkyl substances (PFAS) and PFAS mixtures on lipid metabolism in differentiated HepaRG cells as a model for human hepatocytes
Arch Toxicol. 2023 Dec 20. doi: 10.1007/s00204-023-03649-3. Online ahead of print.ABSTRACTPer- and polyfluoroalkyl substances (PFAS) are environmental contaminants with various adverse health effects in humans including disruption of lipid metabolism. Aim of the present study was to elucidate the molecular mechanisms of PFAS-mediated effects on lipid metabolism in human cells. Here, we examined the impact of a number of PFAS (PFOS, PFOA, PFNA, PFDA, PFHxA, PFBA, PFHxS, PFBS, HFPO-DA, and PMPP) and of some exposure-relevant PFAS mixtures being composed of PFOS, PFOA, PFNA and PFHxS on lipid metabolism in human HepaRG cells,...
Source: Archives of Toxicology - December 20, 2023 Category: Toxicology Authors: Faezeh Sadrabadi Jimmy Alarcan Heike Sprenger Albert Braeuning Thorsten Buhrke Source Type: research

An interactive tool for designing efficient toxicology experiments
Arch Toxicol. 2023 Dec 19. doi: 10.1007/s00204-023-03651-9. Online ahead of print.ABSTRACTThe design of dose-response experiments is an important part of toxicology research. Efficient design of these experiments requires choosing optimal doses and assigning the correct number of subjects to those doses under a given criterion. Optimal design theory provides the tools to find the most efficient experimental designs in terms of cost and statistical efficiency. However, the mathematical details can be distracting and make these designs inaccessible to many toxicologists. To facilitate use of these designs, we present an easy...
Source: Archives of Toxicology - December 19, 2023 Category: Toxicology Authors: William Gertsch Weng Kee Wong Source Type: research

Molecular mechanisms of artificial light at night affecting circadian rhythm disturbance
Arch Toxicol. 2023 Dec 16. doi: 10.1007/s00204-023-03647-5. Online ahead of print.ABSTRACTArtificial light at night (ALAN) pollution has been regarded as a global environmental concern. More than 80% of the global population is exposed to light pollution. Exacerbating this issue, artificially lit outdoor areas are growing by 2.2% per year, while continuously lit areas have brightened by 2.2% each year due to rapid population growth and expanding urbanization. Furthermore, the increasing prevalence of night shift work and smart device usage contributes to the inescapable influence of ALAN. Studies have shown that ALAN can d...
Source: Archives of Toxicology - December 16, 2023 Category: Toxicology Authors: Ting Lei Hui Hua Huiying Du Jie Xia Dandan Xu Wei Liu Yutong Wang Tianyao Yang Source Type: research

Molecular mechanisms of artificial light at night affecting circadian rhythm disturbance
Arch Toxicol. 2023 Dec 16. doi: 10.1007/s00204-023-03647-5. Online ahead of print.ABSTRACTArtificial light at night (ALAN) pollution has been regarded as a global environmental concern. More than 80% of the global population is exposed to light pollution. Exacerbating this issue, artificially lit outdoor areas are growing by 2.2% per year, while continuously lit areas have brightened by 2.2% each year due to rapid population growth and expanding urbanization. Furthermore, the increasing prevalence of night shift work and smart device usage contributes to the inescapable influence of ALAN. Studies have shown that ALAN can d...
Source: Archives of Toxicology - December 16, 2023 Category: Toxicology Authors: Ting Lei Hui Hua Huiying Du Jie Xia Dandan Xu Wei Liu Yutong Wang Tianyao Yang Source Type: research

Molecular mechanisms of artificial light at night affecting circadian rhythm disturbance
Arch Toxicol. 2023 Dec 16. doi: 10.1007/s00204-023-03647-5. Online ahead of print.ABSTRACTArtificial light at night (ALAN) pollution has been regarded as a global environmental concern. More than 80% of the global population is exposed to light pollution. Exacerbating this issue, artificially lit outdoor areas are growing by 2.2% per year, while continuously lit areas have brightened by 2.2% each year due to rapid population growth and expanding urbanization. Furthermore, the increasing prevalence of night shift work and smart device usage contributes to the inescapable influence of ALAN. Studies have shown that ALAN can d...
Source: Archives of Toxicology - December 16, 2023 Category: Toxicology Authors: Ting Lei Hui Hua Huiying Du Jie Xia Dandan Xu Wei Liu Yutong Wang Tianyao Yang Source Type: research

Molecular mechanisms of artificial light at night affecting circadian rhythm disturbance
Arch Toxicol. 2023 Dec 16. doi: 10.1007/s00204-023-03647-5. Online ahead of print.ABSTRACTArtificial light at night (ALAN) pollution has been regarded as a global environmental concern. More than 80% of the global population is exposed to light pollution. Exacerbating this issue, artificially lit outdoor areas are growing by 2.2% per year, while continuously lit areas have brightened by 2.2% each year due to rapid population growth and expanding urbanization. Furthermore, the increasing prevalence of night shift work and smart device usage contributes to the inescapable influence of ALAN. Studies have shown that ALAN can d...
Source: Archives of Toxicology - December 16, 2023 Category: Toxicology Authors: Ting Lei Hui Hua Huiying Du Jie Xia Dandan Xu Wei Liu Yutong Wang Tianyao Yang Source Type: research