The acute toxicity of Novichok's degradation products using quantitative and qualitative toxicology in silico methods
This study focuses on the degradation products of Novichok agents, particularly their potential toxic effects on biological systems. Traditional in vivo methods for toxicity evaluation face ethical and practical constraints, prompting a shift toward in silico toxicology research. In this context, we conducted a comprehensive qualitative and quantitative analysis of acute oral toxicity (AOT) for Novichok degradation products, using various in silico methods, including TEST, CATMoS, ProTox-II, ADMETlab, ACD/Labs Percepta, and QSAR Toolbox. Adopting these methodologies aligns with the 3Rs principle, emphasising Replacement, R...
Source: Archives of Toxicology - March 5, 2024 Category: Toxicology Authors: Maciej Noga Agata Michalska Kamil Jurowski Source Type: research
In vitro and in vivo protective potential of quercetin-3-glucuronide against lipopolysaccharide-induced pulmonary injury through dual activation of nuclear factor-erythroid 2 related factor 2 and autophagy
In this study, the effect of Q3G on antioxidant and autophagy were further investigated. Noncytotoxic doses of Q3G abolished the LPS-caused cell injury, and reactive oxygen species (ROS) generation with inductions in Nrf2-antioxidant signaling. Moreover, Q3G treatment repressed Nrf2 ubiquitination, and enhanced the association of Keap1 and p62 in the LPS-treated cells. Q3G also showed potential in inducing autophagy, as demonstrated by formation of acidic vesicular organelles (AVOs) and upregulation of autophagy factors. Next, the autolysosomes formation and cell survival were decreased by Q3G under pre-treatment with a ly...
Source: Archives of Toxicology - March 4, 2024 Category: Toxicology Authors: Pei-Rong Yu Chiao-Yun Tseng Cheng-Chin Hsu Jing-Hsien Chen Hui-Hsuan Lin Source Type: research
Integrated data from intravital imaging and HPLC-MS/MS analysis reveal large interspecies differences in AFB < sub > 1 < /sub > metabolism in mice and rats
In conclusion, major differences between mice and rats were observed, concerning the nuclear persistence, formation of AFB1-lysine adducts, and the AFB1-guanine adducts.PMID:38436695 | DOI:10.1007/s00204-024-03688-4 (Source: Archives of Toxicology)
Source: Archives of Toxicology - March 4, 2024 Category: Toxicology Authors: Reham Hassan Andrea Gerdemann Benedikt Cramer Zaynab Hobloss Maiju Myllys Daniela Gonz ález Wiebke Albrecht Jannik Veerkamp Adrian Friebel Stefan Hoehme Melanie Esselen Gisela H Degen Hans-Ulrich Humpf Jan G Hengstler Ahmed Ghallab Source Type: research
Genetic variants in m5C modification genes are associated with survival of patients with HBV-related hepatocellular carcinoma
Arch Toxicol. 2024 Mar 4. doi: 10.1007/s00204-024-03687-5. Online ahead of print.ABSTRACTHepatocellular carcinoma (HCC) is one of the most common malignant tumors with a high mortality rate. The 5-methylcytosine (m5C), a type of RNA modification, plays crucial regulatory roles in HCC carcinogenesis, metastasis, and prognosis. However, a few studies have investigated the effect of genetic variants in m5C modification genes on survival of patients with hepatitis B virus (HBV)-related HCC. In the present study, we evaluated associations between 144 SNPs in 15 m5C modification genes and overall survival (OS) in 866 patients wi...
Source: Archives of Toxicology - March 4, 2024 Category: Toxicology Authors: Bowen Chen Moqin Qiu Rongbin Gong Yingchun Liu Zihan Zhou Qiuping Wen Xiaoxia Wei Xiumei Liang Yanji Jiang Peiqin Chen Yuying Wei Qiongguang Huang Qiuyan Mo Qiuling Lin Hongping Yu Source Type: research
Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells
This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (< 2.5 µm) were collected using a high-volume sampler during combustion of traditional Ethiopian biomass fuels: cow dung, eucalyptus wood and eucalyptus charcoal. Diesel exhaust particles (DEP, NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal (136 ng/mg) and DEP (118 ng/mg) (GC-MS). BEAS-2B bronchial epithelial cells and THP-1 derived macro...
Source: Archives of Toxicology - March 1, 2024 Category: Toxicology Authors: Sarah McCarrick Mathilde N Delaval Ulrike M Dauter Annette M Krais Anastasiia Snigireva Asmamaw Abera Karin Broberg Axel C Eriksson Christina Isaxon Anda R Gliga Source Type: research
Effect of cell treatment procedures on in vitro genotoxicity assessment
In conclusion, in the HepaRP cell line, there are some important differences between a one-day acute and a three-day repeated treatment protocol, indicating that different cell treatment procedures may differentiate chemical genotoxic mechanisms of action more efficiently.PMID:38427119 | DOI:10.1007/s00204-024-03690-w (Source: Archives of Toxicology)
Source: Archives of Toxicology - March 1, 2024 Category: Toxicology Authors: Cynthia Recoules Gladys Mirey Marc Audebert Source Type: research
Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells
This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (< 2.5 µm) were collected using a high-volume sampler during combustion of traditional Ethiopian biomass fuels: cow dung, eucalyptus wood and eucalyptus charcoal. Diesel exhaust particles (DEP, NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal (136 ng/mg) and DEP (118 ng/mg) (GC-MS). BEAS-2B bronchial epithelial cells and THP-1 derived macro...
Source: Archives of Toxicology - March 1, 2024 Category: Toxicology Authors: Sarah McCarrick Mathilde N Delaval Ulrike M Dauter Annette M Krais Anastasiia Snigireva Asmamaw Abera Karin Broberg Axel C Eriksson Christina Isaxon Anda R Gliga Source Type: research
Effect of cell treatment procedures on in vitro genotoxicity assessment
In conclusion, in the HepaRP cell line, there are some important differences between a one-day acute and a three-day repeated treatment protocol, indicating that different cell treatment procedures may differentiate chemical genotoxic mechanisms of action more efficiently.PMID:38427119 | DOI:10.1007/s00204-024-03690-w (Source: Archives of Toxicology)
Source: Archives of Toxicology - March 1, 2024 Category: Toxicology Authors: Cynthia Recoules Gladys Mirey Marc Audebert Source Type: research
Excavatolide C/cisplatin combination induces antiproliferation and drives apoptosis and DNA damage in bladder cancer cells
Arch Toxicol. 2024 Feb 29. doi: 10.1007/s00204-024-03699-1. Online ahead of print.ABSTRACTExcavatolide C (EXCC), a marine coral-derived compound, exhibits an antiproliferation effect on bladder cancer cells. The present study evaluated the improvement in the antiproliferation ability of EXCC by co-treatment with cisplatin in bladder cancer cells. EXCC/cisplatin (12.5 and 1 μg/mL) showed higher antiproliferation effects on bladder cancer cells than single treatments (EXCC or cisplatin alone) in the 48 h ATP assay. EXCC/cisplatin also enhanced the increase in subG1, annexin V-mediated apoptosis, and activation of poly (ADP-...
Source: Archives of Toxicology - February 29, 2024 Category: Toxicology Authors: Tsu-Ming Chien Che-Wei Yang Chia-Hung Yen Bi-Wen Yeh Wen-Jeng Wu Jyh-Horng Sheu Hsueh-Wei Chang Source Type: research
Excavatolide C/cisplatin combination induces antiproliferation and drives apoptosis and DNA damage in bladder cancer cells
Arch Toxicol. 2024 Feb 29. doi: 10.1007/s00204-024-03699-1. Online ahead of print.ABSTRACTExcavatolide C (EXCC), a marine coral-derived compound, exhibits an antiproliferation effect on bladder cancer cells. The present study evaluated the improvement in the antiproliferation ability of EXCC by co-treatment with cisplatin in bladder cancer cells. EXCC/cisplatin (12.5 and 1 μg/mL) showed higher antiproliferation effects on bladder cancer cells than single treatments (EXCC or cisplatin alone) in the 48 h ATP assay. EXCC/cisplatin also enhanced the increase in subG1, annexin V-mediated apoptosis, and activation of poly (ADP-...
Source: Archives of Toxicology - February 29, 2024 Category: Toxicology Authors: Tsu-Ming Chien Che-Wei Yang Chia-Hung Yen Bi-Wen Yeh Wen-Jeng Wu Jyh-Horng Sheu Hsueh-Wei Chang Source Type: research
Effects of marine biotoxins on drug-metabolizing cytochrome P450 enzymes and their regulation in mammalian cells
Arch Toxicol. 2024 Feb 28. doi: 10.1007/s00204-024-03694-6. Online ahead of print.ABSTRACTMarine biotoxins are a heterogenous group of natural toxins, which are able to trigger different types of toxicological responses in animals and humans. Health effects arising from exposure to marine biotoxins are ranging, for example, from gastrointestinal symptoms to neurological effects, depending on the individual toxin(s) ingested. Recent research has shown that the marine biotoxin okadaic acid (OA) can strongly diminish the expression of drug-metabolizing cytochrome P450 (CYP) enzymes in human liver cells by a mechanism involvin...
Source: Archives of Toxicology - February 28, 2024 Category: Toxicology Authors: Leonie T D W ürger Jimmy Alarcan Albert Braeuning Source Type: research
Effects of marine biotoxins on drug-metabolizing cytochrome P450 enzymes and their regulation in mammalian cells
Arch Toxicol. 2024 Feb 28. doi: 10.1007/s00204-024-03694-6. Online ahead of print.ABSTRACTMarine biotoxins are a heterogenous group of natural toxins, which are able to trigger different types of toxicological responses in animals and humans. Health effects arising from exposure to marine biotoxins are ranging, for example, from gastrointestinal symptoms to neurological effects, depending on the individual toxin(s) ingested. Recent research has shown that the marine biotoxin okadaic acid (OA) can strongly diminish the expression of drug-metabolizing cytochrome P450 (CYP) enzymes in human liver cells by a mechanism involvin...
Source: Archives of Toxicology - February 28, 2024 Category: Toxicology Authors: Leonie T D W ürger Jimmy Alarcan Albert Braeuning Source Type: research
ATF4 in cellular stress, ferroptosis, and cancer
Arch Toxicol. 2024 Feb 21. doi: 10.1007/s00204-024-03681-x. Online ahead of print.ABSTRACTActivating transcription factor 4 (ATF4), a member of the ATF/cAMP response element-binding (CREB) family, plays a critical role as a stress-induced transcription factor. It orchestrates cellular responses, particularly in the management of endoplasmic reticulum stress, amino acid deprivation, and oxidative challenges. ATF4's primary function lies in regulating gene expression to ensure cell survival during stressful conditions. However, when considering its involvement in ferroptosis, characterized by severe lipid peroxidation and pr...
Source: Archives of Toxicology - February 21, 2024 Category: Toxicology Authors: Hu Tang Rui Kang Jiao Liu Daolin Tang Source Type: research
ATF4 in cellular stress, ferroptosis, and cancer
Arch Toxicol. 2024 Feb 21. doi: 10.1007/s00204-024-03681-x. Online ahead of print.ABSTRACTActivating transcription factor 4 (ATF4), a member of the ATF/cAMP response element-binding (CREB) family, plays a critical role as a stress-induced transcription factor. It orchestrates cellular responses, particularly in the management of endoplasmic reticulum stress, amino acid deprivation, and oxidative challenges. ATF4's primary function lies in regulating gene expression to ensure cell survival during stressful conditions. However, when considering its involvement in ferroptosis, characterized by severe lipid peroxidation and pr...
Source: Archives of Toxicology - February 21, 2024 Category: Toxicology Authors: Hu Tang Rui Kang Jiao Liu Daolin Tang Source Type: research
ATF4 in cellular stress, ferroptosis, and cancer
Arch Toxicol. 2024 Feb 21. doi: 10.1007/s00204-024-03681-x. Online ahead of print.ABSTRACTActivating transcription factor 4 (ATF4), a member of the ATF/cAMP response element-binding (CREB) family, plays a critical role as a stress-induced transcription factor. It orchestrates cellular responses, particularly in the management of endoplasmic reticulum stress, amino acid deprivation, and oxidative challenges. ATF4's primary function lies in regulating gene expression to ensure cell survival during stressful conditions. However, when considering its involvement in ferroptosis, characterized by severe lipid peroxidation and pr...
Source: Archives of Toxicology - February 21, 2024 Category: Toxicology Authors: Hu Tang Rui Kang Jiao Liu Daolin Tang Source Type: research
