Steroid Receptor Coactivator-1 Expression in Pheochromocytoma: Clinicopathologic Correlation and Potential Diagnostic Pitfall
Pheochromocytoma is a relatively uncommon tumor, and the histomorphologic and biochemical features that may portend malignant behavior have poor overall consensus across various proposed classification systems. Steroid receptor coactivator-1 (SRC-1) is a nuclear protein that mediates transcriptional activity. Current diagnostic applications of SRC-1 are limited, and include distinguishing adrenocortical carcinoma (ACC) from renal cell carcinoma, and other mimickers. SRC-1 expression in pheochromocytoma has not been previously studied. Pheochromocytoma cases were retrieved from our Urological Pathology database and expert c...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Prognostic and Predictive Role of CXC Chemokine Receptor 4 in Metastatic Colorectal Cancer Patients
Background: Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide. About 30% of patients present with metastatic disease involving predominantly the liver and a similar percentage will develop distant metastases later after removal of the primary tumor. In metastatic CRC, chemotherapies and biological drugs have prolonged survival for up to 30 months. However, there is a great need for biomarkers predictive of response and prognosis to optimize treatments. CXC chemokine receptor 4 (CXCR4) is a chemokine receptor; it binds to CXCL12 and plays a central role in colon cancer cells’ growth ...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

PD-L1 Expression Is a Favorable Prognostic Marker in Gastric Carcinoma
This study aims to evaluate the expression of PD-L1 in GC samples from Jordanian patients and assess its prognostic role as well as its correlation with clinicopathologic variables. Gastrectomy samples from 96 patients diagnosed with gastric adenocarcinoma were included in the study. Immunohistochemistry assay was employed for PD-L1 testing, and the scoring was based on a combined positive score (CPS). It was found that 66.7% of the study samples were positive for PD-L1 (CPS≥1). The expression of PD-L1 was not significantly associated with any of the assessed clinicopathologic variables; however, it was found to be an in...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Prognostic and Predictive Role of Excision Repair Cross-complementation Group 1 and Thymidylate Synthase in Colorectal Carcinoma Patients Received FOLFOX Chemotherapy: An Immunohistochemical Study
Conclusions: Our results showed that both ERCC-1 and TS are predictive factors for early treatment failure in CRC patients. TS protein is a prognostic factor for disease-free survival rates. This supports the theory that both ERCC-1 and TS can be used to improve chemotherapeutic outcomes in CRC patients. High expression of TS and ERCC-1 helps in the identification of cases that will get fewer benefits from FOLFOX chemotherapy. As an innovative strategy, in these cases, we can use alternative chemotherapeutic regimens or add an extra agent. In addition, Dukes’ classification and lymph node metastasis are predictive fa...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Comparison of the Lymph2Cx Assay and Hans Algorithm in Determining the Cell-of-Origin of Diffuse Large B-Cell Lymphomas, Not Otherwise Specified
In the era of precision medicine, accurate and reproducible assignment of cell-of-origin (COO) in diffuse large B-cell lymphoma patients has become important. The Lymph2Cx assay is accurately determining COO by analyzing RNA expression of 20 selected genes while the Hans algorithm based on immunohistochemistry is the most popular method for routine daily diagnosis. However, there are discrepancies between the 2 methods, which need to be evaluated for better correlation. We prospectively analyzed 156 cases of diffuse large B-cell lymphoma, not otherwise specified to analyze the characteristics of discrepancy groups of COO d...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

EBV-Positive Primary Large B-Cell Lymphoma: The Role of Immunohistochemistry and XPO1 in the Diagnosis of Mediastinal Lymphomas
We present a 19-year-old man with mediastinal mass morphologically consistent with PMBL. The tumor expressed classic immunophenotype, including positivity for CD20, CD19, MAL, OCT2, BOB1, BCL6, CD79a, and subset positivity for CD30. However, the tumor was EBV-positive by in situ hybridization. Next-generation sequencing detected somatic mutations in XPO1 (E571K), SMARCB1 (L356fs), and MYCC (T73A). Although the immunophenotype and XPO1 mutation are characteristic of PMBL, EBV expression is uncommon. Since EBV positivity can occur in rare PMBLs, it should not be the deciding factor in the diagnosis. This is the first EBV-pos...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Collision Tumor of Urothelial Cell Carcinoma and Clear Cell Renal Cell Carcinoma: A Case Report and Review of the Contemporary Literature
We present a case of synchronous renal cell carcinoma and urothelial carcinoma. Our case was a true collision tumor and both components were high grade. Metastasis to the adrenal gland by the urothelial carcinoma was observed. The morphologic impression was confirmed with GATA3 expression by the urothelial carcinoma component and PAX8 expression by the renal cell carcinoma component. We have reviewed the contemporary literature of this unusual dual diagnosis. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Online Articles: Case Reports Source Type: research

Consecutive Immunohistochemical Staining on a Single Slide is Effective in Confirming Melanocytic Derivation
Conclusions: Consecutive staining of melanocytes with SOX-10 and MART-1 may improve diagnostic confidence of melanocyte identification, particularly in detection of single cell, micrometastases in sentinel lymph nodes or in situations where dual immunohistochemical stains may be unavailable. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Online Articles: Case Reports Source Type: research

EPR17341 and A7H6R pan-TRK Immunohistochemistry Result in Highly Different Staining Patterns in a Series of Salivary Gland Tumors
Patients with NTRK-rearranged tumors can be now treated using anti-TRK–targeted therapies making NTRK testing important for treatment choices in patients with advanced cancers. Pan-TRK immunohistochemistry (IHC) could be a valuable premolecular screening strategy in this field. The choice of 1 IHC method or another requires to investigate for intermethod comparison. A high frequency of pan-TRK positive tumors among salivary gland tumors makes these tumors particularly appropriate for such a technical study. In this work, we studied the intermethod agreement for 2 pan-TRK IHC methods (using A7H6R and EPR17341 clones) in a...
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Technical Article Source Type: research

Immunohistochemical Expression Analysis of EpCAM in Hepatoblastomas
The epithelial cell adhesion molecule (EpCAM) is involved in oncogenesis of hepatoblastomas (HBs). Prior genomic profiling studies showed higher EpCAM expression and worse prognosis in HBs containing primitive histotypes, however, this has not been fully addressed from an immunohistochemical perspective. Our goal is to characterize differential EpCAM immunohistochemistry (EpCAM-IHC) among HBs histotypes. We retrieved 62 HBs from 52 patients. EpCAM-IHC was performed (anti-MOC-31, 1:50 dilution; Cell Marque Corporation, Rocklin, CA) and graded in histotypes using the immunoreactive score. The median age of patients was 2 yea...
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Assessment of HPV Risk Type in H&E-stained Biopsy Specimens of the Cervix by Microscopy Image Analysis
Conclusions: Nuclei of HR-HPV types were of higher intensity, contained larger structures, had higher edges, were coarser, rougher, had higher contrast, were larger, and attained more irregular shapes. The proposed DSS indicates that discrimination of HPV risk type from images of H&E-stained biopsy material of the cervix is promising. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

p57 in Hydatidiform Moles: Evaluation of Antibodies and Expression in Various Cell Types
We present a method for evaluating p57 antibodies (Abs) in hydatidiform mole (HM) and demonstrate the results for 4 p57 Abs in various cell types. Five cases of complete HM, diploid with 2 paternal genome sets (CHM;PP), 5 cases of partial HM, triploid with 2 paternal and 1 maternal genome sets (PHM;PPM), and 5 cases of non-HM, with diploid biparental genomes (non-HM;PM) were stained with p57 Abs: 57P06, EP183, KP10, and KP39. Assessment of the fraction of nuclei stained, and the intensity of staining of the nuclei and cytoplasm was performed. For evaluation of the Abs, the observations in cytotrophoblasts, villous stromal ...
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Insulinoma-associated Protein 1 (INSM1) Expression in Small Cell Neuroendocrine Carcinoma of the Urinary Tract
This study examines immunohistochemical expression of INSM1 on whole tissue sections of urinary tract small cell neuroendocrine carcinoma and compares INSM1 expression with established neuroendocrine markers. Immunohistochemical stains for CD56, INSM1, synaptophysin, and chromogranin were performed on 32 cases of small cell neuroendocrine carcinoma of the bladder. Staining was scored for intensity (0: no staining; 1: weak; 2: moderate; 3: strong) and proportion of cells stained (0: 0%; 1:>0% to ≤25%; 2:>25% to ≤50%; 3:>50% to ≤75%; 4:>75% to 100%). INSM1 was positive (intensity 1 to 3 or proportion 1 to 4) in 87% (28...
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Involvement of CHP2 in the Development of Non–Small Cell Lung Cancer and Patients’ Poor Prognosis
In conclusion, the data demonstrated that CHP2 was highly expressed in NSCLC and that it could promote the development of NSCLC, suggesting its potential application for the therapy of NSCLC. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research

Round Robin Evaluation of MET Protein Expression in Lung Adenocarcinomas Improves Interobserver Concordance
Discussion: We found that the consistency of MET immunohistochemistry scoring is improved by continuous training and communication between pathologists. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2020 Category: Chemistry Tags: Research Articles Source Type: research