Log in to search using one of your social media accounts:

 

Targets and Receptor of Sexual Transmission of Zika Virus
No abstract available (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Online Articles: Letter to the Editor Source Type: research

Primary Epithelioid Angiosarcoma of Finger Masquerading as Epithelioid Hemangioma: Report of a Case and Analysis of Mutational Pattern in Epithelioid Hemangiomas and Angiosarcomas by Next-generation Sequencing
Conclusions: We present an epithelioid AS mimicking EH. We report novel somatic variants in EHs and AS. Benign variants may not be associated with development of these tumors. Whereas, deleterious variants, especially PTPN11 c.226G>A, may be linked to tumorigenesis of AS. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Online Articles: Research Articles Source Type: research

Cell Polarity Reversal Distinguishes True Micropapillary Growth From Retraction Artifact in Invasive Urothelial Carcinoma
Focal micropapillary features in invasive urothelial carcinoma is sometimes difficult to distinguish from retraction artifact morphologically. Cell polarity reversal has been demonstrated in micropapillary tumors by epithelial membrane antigen (EMA) immunostaining. We have previously described the use of E-cadherin as a cell polarity marker in ovarian micropapillary serous borderline tumors. The aim of this study was to evaluate the utility of immunohistochemistry for EMA and E-cadherin in differentiating micropapillary urothelial carcinoma from retraction artifact. We identified 29 invasive urothelial carcinomas with micr...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Online Articles: Research Articles Source Type: research

Usage of DNA Fingerprinting Technology for Quality Control in Molecular Lab Bench Work
One of the major quality assurance (QA) goals in many molecular laboratories is to avoid sample pipetting errors on the lab bench; especially when pipetting into multiwell plates. A pipetting error can cause a switch in patient samples, which can lead to recording the wrong results for the patient samples involved. Such pipetting errors are difficult to identify when it happens in lab bench work. DNA fingerprinting is a powerful tool in determining sample identities. Our laboratory has explored the usage of this technology in our QA process and successfully established that DNA fingerprinting can be used to monitor possibl...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Technical Article Source Type: research

A Comparative Immunohistochemical Study of Epithelial Membrane Antigen and NHERF1/EBP50 in the Diagnosis of Ependymomas
Ependymomas are gliomas that recapitulate normal ependymal cells. The epithelial membrane antigen (EMA) shows “dot-like” and “ring-like” staining patterns, highlighting “microlumens” or intracytoplasmic rosettes, a pathognomonic ultrastructural feature. NHERF1/EBP50, an adaptor protein localized at the apical plasma membrane of human epithelia, has been found to localize to these microlumens. We aimed to analyze the staining patterns of EMA and EBP50 in ependymomas and other tumors, and thereby compare their diagnostic utility. Sixty-three ependymomas of different grades and 44 nonependy...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

The Expression Pattern of p120-Catenin is Associated With Acquired Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Non–Small Cell Lung Cancer
Conclusions: Therefore, this study demonstrates that the expression pattern of p120ctn is associated with acquired resistance to EGFR-TKIs in lung cancer, providing information toward addressing the problem of drug resistance in patients with non–small cell lung cancer. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

New MYC IHC Classifier Integrating Quantitative Architecture Parameters to Predict MYC Gene Translocation in Diffuse Large B-Cell Lymphoma
A new automated MYC IHC classifier based on bivariate logistic regression is presented. The predictor relies on image analysis developed with the open-source ImageJ platform. From a histologic section immunostained for MYC protein, 2 dimensionless quantitative variables are extracted: (a) relative distance between nuclei positive for MYC IHC based on euclidean minimum spanning tree graph and (b) coefficient of variation of the MYC IHC stain intensity among MYC IHC-positive nuclei. Distance between positive nuclei is suggested to inversely correlate MYC gene rearrangement status, whereas coefficient of variation is suggeste...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

Malignant Melanoma Presenting as Thoracic Midline Malignancy: Clinicopathologic and Molecular Features
In this study, we provide a detailed evaluation of 6 cases of MM presenting as thoracic midline malignancy. The tumors occurred in 6 white patients (5 males and 1 female; medium age, 71.5 y; range, 55 to 81 y). At presentation, the disease was confined to the chest (6/6) with 4 cases subsequently showing brain metastasis. All 6 tumors featured epithelioid and 1 case also spindle cell morphology. Three of 6 tumors had melanin and all 6 expressed melanocytic markers. Molecular characterization showed 2 of 6 tumors with BRAF“hot spot” genetic alterations (both with BRAFV600K) and 1 with NF1mutation. All 6 patients...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

SP174 Antibody Lacks Specificity for NRAS Q61R and Cross-Reacts With HRAS and KRAS Q61R Mutant Proteins in Malignant Melanoma
HRAS, KRAS, and NRAS, highly homologous proteins, are often mutationally activated in cancer. Usually, mutations cluster in codons 12, 13, and 61 and are detected by molecular genetic testing of tumor DNA. Recently, immunohistochemistry with SP174 antibody has been introduced to detect NRAS Q61R-mutant protein. Studies on malignant melanomas showed that such an approach could be a viable alternative to molecular genetic testing. This investigation was undertaken to evaluate the value of SP174 immunohistochemistry for detection of NRAS Q61R-mutant isoform. Two hundred ninety-two malignant melanomas were evaluated using Leic...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

HER2 Status in Gastroesophageal Adenocarcinomas: Correlation Between Immunohistochemistry and Fluorescence In Situ Hybridization Methodologies
Semiquantitative immunohistochemistry (IHC) is commonly used in combination with fluorescence in situ hybridization (FISH) to detect HER2 amplification in gastroesophageal adenocarcinomas. Most laboratories apply these tests in a sequential algorithm, using IHC as a frontline test and reserving FISH for IHC-equivocal cases. To gain a better understanding of the concordance of IHC and FISH results at our institution, we identified all gastroesophageal adenocarcinomas at our institution tested for HER2 (n=125). Matched IHC and FISH were available for 116 cases (94%). Cases consisted of adenocarcinoma of the distal esophagus ...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

The Use of a Novel Immunohistochemical Triple Cocktail in the Subclassification of Resected Non–Small Cell Lung Carcinomas: A Comparative Study With Morphology and Traditional Immunohistochemistry
Therapy for non–small cell lung carcinoma (NSCLC) is currently determined by histologic subtype and the presence or absence of actionable mutations. Accurate subclassification is therefore essential for appropriate selection of cases for molecular studies and guiding treatment. The gold standard for subclassification of NSCLC is identification of differentiating morphologic features in correlation with diagnostic immunohistochemistry (IHC) in cases of poorly differentiated carcinoma. Whereas Napsin A, TTF1, and p40 antibodies have been used individually for the subtyping of NSCLC, few studies have examined the 3 in c...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

Performance of 4 Immunohistochemical Phosphohistone H3 Antibodies for Marking Mitotic Figures in Breast Cancer
Background: Phosphohistone H3 (PHH3) has been suggested to facilitate and improve mitotic activity assessment in breast cancer and other tumor entities, but the reliability of respective immunohistochemical antibodies has not yet been compared for routine purposes. Our aim was to test the performance of 4 different PHH3 antibodies on a series of highly proliferating breast cancers with good preservation of morphology. Methods: Four commercially available PHH3 antibodies were tested on 9 grade 3 invasive breast cancers processed in the same batch. We analyzed the number of antibody stained and nonstained mitotic figure...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

Expression Patterns of Biomarkers in Primary Tumors and Corresponding Metastases in Breast Cancer
Tumor heterogeneity has been shown for several cancers including breast cancer (BC). Despite the fact that expression of tumor markers may change throughout the metastatic process, rebiopsies at the time of recurrence are still not performed routinely at all institutions. The aims of the study were to evaluate changes in biomarker profiles during the metastatic process and to investigate whether previous anthracycline or endocrine therapy given in the adjuvant setting could affect the biomarker profile in metastatic lesions. We investigated the expression pattern of ER, HER2, TOP2a, TOP1, p53, Bcl-2, and Ki-67 in 110 paire...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Review Article Source Type: research

Development of a Diagnostic Programmed Cell Death 1-Ligand 1 Immunohistochemistry Assay for Nivolumab Therapy in Melanoma
We report validation of PD-L1 IHC 28-8 pharmDx for PD-L1 detection on formalin-fixed, paraffin-embedded human melanoma specimens using Autostainer Link 48. A prevalence assessment of 104 melanoma specimens indicated that PD-L1 was detected across the full expression level range (0% to 100% of tumor cells). Assay robustness and precision studies were conducted at Agilent Technologies, with additional reproducibility studies performed at 3 external laboratories. Precision studies evaluated at ≥1% and ≥5% expression levels revealed a range of average negative agreement from 89.5%, 95% CI (83.2, 93.6) to 100%, 95% CI (97...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

Expression of GHRH-R, a Potentially Targetable Biomarker, in Triple-negative Breast Cancer
In this study, we investigated the expression of GHRH-R in triple-negative breast cancers (TNBC). As TNBCs are morphologically and immunophenotypically heterogenous, the staining results were also correlated with the histologic subtypes of these tumors. Materials and Methods: On the basis of histomorphology and immunophenotype, 134 cases of primary TNBCs were further subdivided into medullary, metaplastic, apocrine, and invasive ductal carcinomas of no special type (IDC-NST). Immunohistochemistry for GHRH-R was performed on paraffin sections and the staining results were assessed semiquantitatively as negative, low expr...
Source: Applied Immunohistochemistry and Molecular Morphology - January 1, 2018 Category: Chemistry Tags: Research Articles Source Type: research

Targets and Receptor of Sexual Transmission of Zika Virus
No abstract available (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Online Articles: Letter to the Editor Source Type: research

Primary Epithelioid Angiosarcoma of Finger Masquerading as Epithelioid Hemangioma: Report of a Case and Analysis of Mutational Pattern in Epithelioid Hemangiomas and Angiosarcomas by Next-generation Sequencing
Conclusions: We present an epithelioid AS mimicking EH. We report novel somatic variants in EHs and AS. Benign variants may not be associated with development of these tumors. Whereas, deleterious variants, especially PTPN11 c.226G>A, may be linked to tumorigenesis of AS. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Online Articles: Research Articles Source Type: research

Cell Polarity Reversal Distinguishes True Micropapillary Growth From Retraction Artifact in Invasive Urothelial Carcinoma
Focal micropapillary features in invasive urothelial carcinoma is sometimes difficult to distinguish from retraction artifact morphologically. Cell polarity reversal has been demonstrated in micropapillary tumors by epithelial membrane antigen (EMA) immunostaining. We have previously described the use of E-cadherin as a cell polarity marker in ovarian micropapillary serous borderline tumors. The aim of this study was to evaluate the utility of immunohistochemistry for EMA and E-cadherin in differentiating micropapillary urothelial carcinoma from retraction artifact. We identified 29 invasive urothelial carcinomas with micr...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Online Articles: Research Articles Source Type: research

Usage of DNA Fingerprinting Technology for Quality Control in Molecular Lab Bench Work
One of the major quality assurance (QA) goals in many molecular laboratories is to avoid sample pipetting errors on the lab bench; especially when pipetting into multiwell plates. A pipetting error can cause a switch in patient samples, which can lead to recording the wrong results for the patient samples involved. Such pipetting errors are difficult to identify when it happens in lab bench work. DNA fingerprinting is a powerful tool in determining sample identities. Our laboratory has explored the usage of this technology in our QA process and successfully established that DNA fingerprinting can be used to monitor possibl...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Techincal Article Source Type: research

A Comparative Immunohistochemical Study of Epithelial Membrane Antigen and NHERF1/EBP50 in the Diagnosis of Ependymomas
Ependymomas are gliomas that recapitulate normal ependymal cells. The epithelial membrane antigen (EMA) shows “dot-like” and “ring-like” staining patterns, highlighting “microlumens” or intracytoplasmic rosettes, a pathognomonic ultrastructural feature. NHERF1/EBP50, an adaptor protein localized at the apical plasma membrane of human epithelia, has been found to localize to these microlumens. We aimed to analyze the staining patterns of EMA and EBP50 in ependymomas and other tumors, and thereby compare their diagnostic utility. Sixty-three ependymomas of different grades and 44 nonependy...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

The Expression Pattern of p120-Catenin is Associated With Acquired Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Non–Small Cell Lung Cancer
Conclusions: Therefore, this study demonstrates that the expression pattern of p120ctn is associated with acquired resistance to EGFR-TKIs in lung cancer, providing information toward addressing the problem of drug resistance in patients with non–small cell lung cancer. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

New MYC IHC Classifier Integrating Quantitative Architecture Parameters to Predict MYC Gene Translocation in Diffuse Large B-Cell Lymphoma
A new automated MYC IHC classifier based on bivariate logistic regression is presented. The predictor relies on image analysis developed with the open-source ImageJ platform. From a histologic section immunostained for MYC protein, 2 dimensionless quantitative variables are extracted: (a) relative distance between nuclei positive for MYC IHC based on euclidean minimum spanning tree graph and (b) coefficient of variation of the MYC IHC stain intensity among MYC IHC-positive nuclei. Distance between positive nuclei is suggested to inversely correlate MYC gene rearrangement status, whereas coefficient of variation is suggeste...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Malignant Melanoma Presenting as Thoracic Midline Malignancy: Clinicopathologic and Molecular Features
In this study, we provide a detailed evaluation of 6 cases of MM presenting as thoracic midline malignancy. The tumors occurred in 6 white patients (5 males and 1 female; medium age, 71.5 y; range, 55 to 81 y). At presentation, the disease was confined to the chest (6/6) with 4 cases subsequently showing brain metastasis. All 6 tumors featured epithelioid and 1 case also spindle cell morphology. Three of 6 tumors had melanin and all 6 expressed melanocytic markers. Molecular characterization showed 2 of 6 tumors with BRAF“hot spot” genetic alterations (both with BRAFV600K) and 1 with NF1mutation. All 6 patients...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

SP174 Antibody Lacks Specificity for NRAS Q61R and Cross-Reacts With HRAS and KRAS Q61R Mutant Proteins in Malignant Melanoma
HRAS, KRAS, and NRAS, highly homologous proteins, are often mutationally activated in cancer. Usually, mutations cluster in codons 12, 13, and 61 and are detected by molecular genetic testing of tumor DNA. Recently, immunohistochemistry with SP174 antibody has been introduced to detect NRAS Q61R-mutant protein. Studies on malignant melanomas showed that such an approach could be a viable alternative to molecular genetic testing. This investigation was undertaken to evaluate the value of SP174 immunohistochemistry for detection of NRAS Q61R-mutant isoform. Two hundred ninety-two malignant melanomas were evaluated using Leic...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

HER2 Status in Gastroesophageal Adenocarcinomas: Correlation Between Immunohistochemistry and Fluorescence In Situ Hybridization Methodologies
Semiquantitative immunohistochemistry (IHC) is commonly used in combination with fluorescence in situ hybridization (FISH) to detect HER2 amplification in gastroesophageal adenocarcinomas. Most laboratories apply these tests in a sequential algorithm, using IHC as a frontline test and reserving FISH for IHC-equivocal cases. To gain a better understanding of the concordance of IHC and FISH results at our institution, we identified all gastroesophageal adenocarcinomas at our institution tested for HER2 (n=125). Matched IHC and FISH were available for 116 cases (94%). Cases consisted of adenocarcinoma of the distal esophagus ...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

The Use of a Novel Immunohistochemical Triple Cocktail in the Subclassification of Resected Non–Small Cell Lung Carcinomas: A Comparative Study With Morphology and Traditional Immunohistochemistry
Therapy for non–small cell lung carcinoma (NSCLC) is currently determined by histologic subtype and the presence or absence of actionable mutations. Accurate subclassification is therefore essential for appropriate selection of cases for molecular studies and guiding treatment. The gold standard for subclassification of NSCLC is identification of differentiating morphologic features in correlation with diagnostic immunohistochemistry (IHC) in cases of poorly differentiated carcinoma. Whereas Napsin A, TTF1, and p40 antibodies have been used individually for the subtyping of NSCLC, few studies have examined the 3 in c...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Performance of 4 Immunohistochemical Phosphohistone H3 Antibodies for Marking Mitotic Figures in Breast Cancer
Background: Phosphohistone H3 (PHH3) has been suggested to facilitate and improve mitotic activity assessment in breast cancer and other tumor entities, but the reliability of respective immunohistochemical antibodies has not yet been compared for routine purposes. Our aim was to test the performance of 4 different PHH3 antibodies on a series of highly proliferating breast cancers with good preservation of morphology. Methods: Four commercially available PHH3 antibodies were tested on 9 grade 3 invasive breast cancers processed in the same batch. We analyzed the number of antibody stained and nonstained mitotic figure...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Expression Patterns of Biomarkers in Primary Tumors and Corresponding Metastases in Breast Cancer
Tumor heterogeneity has been shown for several cancers including breast cancer (BC). Despite the fact that expression of tumor markers may change throughout the metastatic process, rebiopsies at the time of recurrence are still not performed routinely at all institutions. The aims of the study were to evaluate changes in biomarker profiles during the metastatic process and to investigate whether previous anthracycline or endocrine therapy given in the adjuvant setting could affect the biomarker profile in metastatic lesions. We investigated the expression pattern of ER, HER2, TOP2a, TOP1, p53, Bcl-2, and Ki-67 in 110 paire...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Review Article Source Type: research

Development of a Diagnostic Programmed Cell Death 1-Ligand 1 Immunohistochemistry Assay for Nivolumab Therapy in Melanoma
We report validation of PD-L1 IHC 28-8 pharmDx for PD-L1 detection on formalin-fixed, paraffin-embedded human melanoma specimens using Autostainer Link 48. A prevalence assessment of 104 melanoma specimens indicated that PD-L1 was detected across the full expression level range (0% to 100% of tumor cells). Assay robustness and precision studies were conducted at Agilent Technologies, with additional reproducibility studies performed at 3 external laboratories. Precision studies evaluated at ≥1% and ≥5% expression levels revealed a range of average negative agreement from 89.5%, 95% CI (83.2, 93.6) to 100%, 95% CI (97...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Reserch Articles Source Type: research

Expression of GHRH-R, a Potentially Targetable Biomarker, in Triple-negative Breast Cancer
In this study, we investigated the expression of GHRH-R in triple-negative breast cancers (TNBC). As TNBCs are morphologically and immunophenotypically heterogenous, the staining results were also correlated with the histologic subtypes of these tumors. Materials and Methods: On the basis of histomorphology and immunophenotype, 134 cases of primary TNBCs were further subdivided into medullary, metaplastic, apocrine, and invasive ductal carcinomas of no special type (IDC-NST). Immunohistochemistry for GHRH-R was performed on paraffin sections and the staining results were assessed semiquantitatively as negative, low expr...
Source: Applied Immunohistochemistry and Molecular Morphology - December 6, 2017 Category: Chemistry Tags: Reserch Articles Source Type: research

Clear Cell Variant of Calcifying Epithelial Odontogenic Tumor: A Case Report and Preliminary Immunohistochemical Study of the SHH Pathway
No abstract available (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Online Articles: Letters to the Editor Source Type: research

The Family of β-Catenin Ovarian Tumors
No abstract available (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Online Articles: Letters to the Editor Source Type: research

Multifocal Supra and Infratentorial Medulloblastoma in an Adult: Histologic, Immunohistochemical, and Molecular Evaluation of a Rare Case and Review of the Literature
Multifocal medulloblastomas (MMBs) in adults are exceedingly rare with only 5 reported cases to date. Medulloblastoma in adult differ from its childhood counterpart by being more often lateral in location, desmoplastic in morphology, and better in clinical prognosis. Little is known, however, about the characteristic features of MMB. This is particularly true for their molecular profiles. To date, molecular characteristics of multifocal medulloblastoma have been reported only once. Here, we present the second case of multifocal medulloblastoma along with its detailed morphology, imaging features, and molecular profiles wit...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Online Articles: Case Report Source Type: research

Evaluation of Mast Cell Density in the Tumor Microenvironment in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma
The objective of this study was to compare mast cell density (MCD) in oral epithelial dysplasias (OED) and oral squamous cell carcinoma (OSCC) and determine its correlation with clinical and histopathologic parameters and the degree of tumor differentiation. Thirty OSCC samples, 14 OED samples, and 4 non-neoplastic oral mucosa samples were analyzed by immunohistochemistry to determine MCD based on the expression of MC tryptase. In addition, MCs were categorized morphologically into degranulated and granulated cells. MCD was significantly higher in OSCC lesions with a greater degree of differentiation (P=0.04). No significa...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Online Articles: Research Article Source Type: research

Paraffin-embedded Tissue Fragment Suspension (PETFS): A Novel Method for Quality Control Preparation in Immunohistochemistry
Immunohistochemistry (IHC) is one of the most important adjunctive techniques in surgical pathology. Quality controls are essential for staining interpretation. The most common controls are cut from the formalin-fixed, paraffin-embedded tissue blocks in advance. In contrast, we developed paraffin-embedded tissue fragment suspension (PETFS), a novel method in liquid form, for quality control preparation. The liquid form controls were cut from the donor formalin-fixed, paraffin-embedded paraffin blocks, stored in the 4°C fridge easily, and added to the top and bottom of the test slide directly by pipetting. The tissue fr...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Hepatocyte-like Versus Mesenchymal Stem Cells in CCl4-induced Liver Fibrosis
It is still a matter of debate as to whether in vitro mesenchymal stem cell (MSC)-derived hepatocytes may efficiently repopulate a host liver to provide adequate functional substitution. The aim of this study is to assess the efficacy and consistency of in vitro hepatic differentiation from Wharton jelly–derived MSCs, and to validate their therapeutic potential in experimentally induced liver fibrosis compared with nondifferentiated MSCs. Forty adult male albino rats were divided into 4 main groups: (I) normal control group; (II) carbon tetrachloride (CCl4)-treated group (injected CCl4 solution twice a week for 8 wk)...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Parafibromin, APC, and MIB-1 Are Useful Markers for Distinguishing Parathyroid Carcinomas From Adenomas
Background: Differentiation of parathyroid carcinoma (PC) from parathyroid adenoma (PA) relies solely on the pathologic determination of invasion of surrounding structures and/or distant metastasis. Parathyroid lesions with atypical histologic features with no demonstration of invasion or metastasis present a diagnostic dilemma. Different authors report a parafibromin and adenomatous polyposis coli (APC) loss or reduction in PC cases. High proliferative activity of MIB-1 and increased galectin 3 expression are reported in PC. There is no clear cutoff for the sensitivity, specificity, or predictive value for all these mar...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Interleukin 18 -607 A/C Gene Polymorphism is Associated With Susceptibility to IgA Nephropathy in a Chinese Han Population
The association between a single-nucleotide polymorphism (SNP; -607A/C) in the promoter region of the IL-18 gene with IL-18 promoter transcription activity and the level of IL-18 protein production has been observed repeatedly. However, the association between the IL-18-607A/C (rs1946518) promoter polymorphism and IgA nephropathy (IgAN) in a Chinese Han population is still unclear. A total of 166 patients with renal biopsy-proven IgAN and 198 healthy controls were recruited for this study. The SNP was genotyped by the polymerase chain reaction and the high-resolution melting method. Clinical characteristics and pathology g...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Invasive Duct Carcinoma of the Breast With Dominant Signet-Ring Cell Differentiation: A Microsatellite Stable Tumor With Aggressive Behavior
Conclusions: Signet-ring cell differentiation in IDC, NST, is rare and associated with a high histologic grade. Lymph node metastasis and distant metastasis are common, indicating an aggressive clinical behavior. Thus, they should be recognized separately as they may warrant aggressive management. However, these are microsatellite-stable tumors in contrast to signet-ring cell tumors of other organs. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Application of MDM2 Fluorescence In Situ Hybridization and Immunohistochemistry in Distinguishing Dedifferentiated Liposarcoma From Other High-grade Sarcomas
Distinguishing dedifferentiated liposarcoma (DDLPS) from other high-grade spindle and pleomorphic sarcomas is important because of better prognosis in case of DDLPS. MDM2 amplification, a genetic abnormality of well-differentiated liposarcoma, is known to be present not only in DDLPS, but also in some other sarcomas. To differentiate DDLPS, we investigated MDM2 amplification and expression in high-grade spindle sarcomas. Eighty-five cases of nonlipogenic high-grade sarcomas, diagnosed between 2008 and 2011, were investigated. Tissue microarray, immunohistochemistry, and fluorescence in situ hybridization for MDM2 were perf...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

The Diagnostic Role of SPINK1 in Differentiating Hepatocellular Carcinoma From Nonmalignant Lesions
Conclusions: SPINK1 can be used to differentiate between a WD-HCC and a HGDN with high diagnostic validity. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

A Selected Immunohistochemical Panel Aids in Differential Diagnosis and Prognostic Stratification of Subtypes of High-grade Endometrial Carcinoma: A Clinicopathologic and Immunohistochemical Study at a Single Institution
This study aimed to investigate whether a selected immunohistochemical panel (estrogen receptor, p53, ARID1A, PPP2R1A, HNF-1β) could contribute to the diagnostic process of high-grade endometrial carcinomas (HG-ECs). We also aimed to analyze the correlation of these immunohistochemical results with several morphologic variables and survival data. After revising the diagnosis of 78 HG-ECs, immunohistochemical analysis was performed for each case. After immunohistochemical analysis, a specific diagnosis of prototypic HG-EC was established in most of the cases that were uncertain due to morphologic ambiguity. In the univ...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

New Robust and Reproducible Stereological IHC Ki67 Breast Cancer Proliferative Assessment to Replace Traditional Biased Labeling Index
There is a pressing need for an objective decision tool to guide therapy for breast cancer patients that are estrogen receptor positive and HER2/neu negative. This subset of patients contains a mixture of luminal A and B tumors with good and bad outcomes, respectively. The 2 main current tools are on the basis of immunohistochemistry (IHC) or gene expression, both of which rely on the expression of distinct molecular groups that reflect hormone receptors, HER2/neu status, and most importantly, proliferation. Despite the success of a proprietary molecular test, definitive superiority of any method has not yet been demonstra...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Cellular Targets and Receptor of Sexual Transmission of Zika Virus
Study Question: What is the mechanism of sexual transmission of Zika virus (ZIKV)? Summary Answer: By utilizing exquisite reverse transcriptase–initiated in situ polymerase chain reaction (RT-in situ PCR), which enables an improved visualization of spermatozoa’s subcellular compartment, we precisely localized the mid-piece of sperm that carry receptors for ZIKV. What is Already Known: ZIKV is transmitted sexually and recent studies have verified ZIKV presence in semen of previously Zika-infected patients for>6-month postinfection when ZIKV had disappeared from blood, saliva, and urine. Strong serial ...
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

NordiQC Assessments of Low Molecular Weight Keratin 8/18 Immunoassays
This paper is number 2 in a series developed through a partnership between ISIMM and NordiQC for the purpose of reporting research assessing the performance characteristics of immunoassays in an external proficiency testing program. (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Research Articles Source Type: research

Controls, Fit-for-purpose Assays, Verification Versus Validation, and Tissue Tools for IHC: Announcing a Workshop From the International Society for Immunohistochemistry and Molecular Morphology, Held at the 12th Annual Retreat for Applied Immunohistochemistry and Molecular Morphology, February 4, 2018
No abstract available (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - November 1, 2017 Category: Chemistry Tags: Editorial Source Type: research

Clinicopathologic Correlates of Helicobacter pylori in Gastric Mucosa
No abstract available (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2017 Category: Chemistry Tags: Online Articles: Letters to the Editor Source Type: research

Sporadic Burkitt Lymphoma Presenting as Intestinal Polyposis in a Child
No abstract available (Source: Applied Immunohistochemistry and Molecular Morphology)
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2017 Category: Chemistry Tags: Online Articles: Letters to the Editor Source Type: research

Qualitative Comparison Between Carrier-based and Classical Tissue Microarrays
Tissue microarrays (TMAs) are commonly used in biomarker research. To enhance the efficacy of TMAs and to avoid floating or folding of tissue cores, various improvements such as the application of carriers and melting techniques have been proposed. Compared with classical TMAs (cTMAs), carrier-based TMAs (cbTMAs) have been shown to have several advantages including sample handling and sectioning. Up to now, little is known about the efficacy and quality of cbTMAs compared with cTMAs. Thus, we set out to compare both types systematically. We constructed 5 spleen-based TMAs and 5 cTMAs with 10×10 different tissue types...
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2017 Category: Chemistry Tags: Online Articles: Research Article Source Type: research

False ALK Rearrangement Signals in Inflammatory Cells: A Pitfall in the Interpretation of ALK Fluorescence In Situ Hybridization in Non–Small Cell Lung Cancer
Searching for ALK rearrangements has now become mandatory for the treatment of patients with advanced non–small cell lung cancer with anti-ALK-targeted therapy. The fluorescence in situ hybridization test is considered the “gold standard” to diagnose ALK-rearranged tumors. Nevertheless, some technical pitfalls may cause false-positive signals mimicking ALK rearrangements. In this technical article, we point out the importance of taking into account both histopathologic and ALK immunohistochemical features to interpret ALK fluorescence in situ hybridization analyses in inflammatory and necrotic tumors. Thi...
Source: Applied Immunohistochemistry and Molecular Morphology - October 1, 2017 Category: Chemistry Tags: Technical Article Source Type: research