New insights into the neuraminidase-mediated hemagglutination activity of influenza A(H3N2) viruses
In this study, we examined the effect of natural amino acid polymorphism at position 150 on NA-mediated hemagglutination. Using the A/Puerto Rico/8/34 backbone, we generated a comprehensive panel of recombinant A(H3N2) viruses that have different NAs but shared an HA that displays poor binding to red blood cells (RBCs). None of the tested substitutions at 150 (C, H, L, R, and S) promoted NA-binding. However, we identified two new determinants of NA-binding, Q136K and T439R, that emerged during virus culturing. Similar to T148I, both Q136K and T439R reduced NA enzyme activity by 48-86% and inhibition (14- to 173-fold) by th...
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Rongyuan Gao Philippe Noriel Q Pascua Ha T Nguyen Anton Chesnokov Chloe Champion Vasiliy P Mishin Dave E Wentworth Larisa V Gubareva Source Type: research

Corrigendum to "Crystal structure and cap binding analysis of the methyltransferase of langat virus" [Antivir. Res. 208 (2022) 105459]
Antiviral Res. 2023 Sep 15:105717. doi: 10.1016/j.antiviral.2023.105717. Online ahead of print.NO ABSTRACTPMID:37718128 | DOI:10.1016/j.antiviral.2023.105717 (Source: Antiviral Research)
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Ruixue Li Ziping Niu Yujie Liu Xue Bai Deping Wang Chen Chen Source Type: research

New insights into the neuraminidase-mediated hemagglutination activity of influenza A(H3N2) viruses
In this study, we examined the effect of natural amino acid polymorphism at position 150 on NA-mediated hemagglutination. Using the A/Puerto Rico/8/34 backbone, we generated a comprehensive panel of recombinant A(H3N2) viruses that have different NAs but shared an HA that displays poor binding to red blood cells (RBCs). None of the tested substitutions at 150 (C, H, L, R, and S) promoted NA-binding. However, we identified two new determinants of NA-binding, Q136K and T439R, that emerged during virus culturing. Similar to T148I, both Q136K and T439R reduced NA enzyme activity by 48-86% and inhibition (14- to 173-fold) by th...
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Rongyuan Gao Philippe Noriel Q Pascua Ha T Nguyen Anton Chesnokov Chloe Champion Vasiliy P Mishin Dave E Wentworth Larisa V Gubareva Source Type: research

Corrigendum to "Crystal structure and cap binding analysis of the methyltransferase of langat virus" [Antivir. Res. 208 (2022) 105459]
Antiviral Res. 2023 Sep 15:105717. doi: 10.1016/j.antiviral.2023.105717. Online ahead of print.NO ABSTRACTPMID:37718128 | DOI:10.1016/j.antiviral.2023.105717 (Source: Antiviral Research)
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Ruixue Li Ziping Niu Yujie Liu Xue Bai Deping Wang Chen Chen Source Type: research

New insights into the neuraminidase-mediated hemagglutination activity of influenza A(H3N2) viruses
In this study, we examined the effect of natural amino acid polymorphism at position 150 on NA-mediated hemagglutination. Using the A/Puerto Rico/8/34 backbone, we generated a comprehensive panel of recombinant A(H3N2) viruses that have different NAs but shared an HA that displays poor binding to red blood cells (RBCs). None of the tested substitutions at 150 (C, H, L, R, and S) promoted NA-binding. However, we identified two new determinants of NA-binding, Q136K and T439R, that emerged during virus culturing. Similar to T148I, both Q136K and T439R reduced NA enzyme activity by 48-86% and inhibition (14- to 173-fold) by th...
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Rongyuan Gao Philippe Noriel Q Pascua Ha T Nguyen Anton Chesnokov Chloe Champion Vasiliy P Mishin Dave E Wentworth Larisa V Gubareva Source Type: research

Corrigendum to "Crystal structure and cap binding analysis of the methyltransferase of langat virus" [Antivir. Res. 208 (2022) 105459]
Antiviral Res. 2023 Sep 15:105717. doi: 10.1016/j.antiviral.2023.105717. Online ahead of print.NO ABSTRACTPMID:37718128 | DOI:10.1016/j.antiviral.2023.105717 (Source: Antiviral Research)
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Ruixue Li Ziping Niu Yujie Liu Xue Bai Deping Wang Chen Chen Source Type: research

New insights into the neuraminidase-mediated hemagglutination activity of influenza A(H3N2) viruses
In this study, we examined the effect of natural amino acid polymorphism at position 150 on NA-mediated hemagglutination. Using the A/Puerto Rico/8/34 backbone, we generated a comprehensive panel of recombinant A(H3N2) viruses that have different NAs but shared an HA that displays poor binding to red blood cells (RBCs). None of the tested substitutions at 150 (C, H, L, R, and S) promoted NA-binding. However, we identified two new determinants of NA-binding, Q136K and T439R, that emerged during virus culturing. Similar to T148I, both Q136K and T439R reduced NA enzyme activity by 48-86% and inhibition (14- to 173-fold) by th...
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Rongyuan Gao Philippe Noriel Q Pascua Ha T Nguyen Anton Chesnokov Chloe Champion Vasiliy P Mishin Dave E Wentworth Larisa V Gubareva Source Type: research

Corrigendum to "Crystal structure and cap binding analysis of the methyltransferase of langat virus" [Antivir. Res. 208 (2022) 105459]
Antiviral Res. 2023 Sep 15:105717. doi: 10.1016/j.antiviral.2023.105717. Online ahead of print.NO ABSTRACTPMID:37718128 | DOI:10.1016/j.antiviral.2023.105717 (Source: Antiviral Research)
Source: Antiviral Research - September 17, 2023 Category: Virology Authors: Ruixue Li Ziping Niu Yujie Liu Xue Bai Deping Wang Chen Chen Source Type: research

Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase
Antiviral Res. 2023 Sep 9;218:105716. doi: 10.1016/j.antiviral.2023.105716. Online ahead of print.ABSTRACTSangivamycin (S) is an adenosine (A) nucleoside analog with low nanomolar antiviral activity against SARS-CoV-2 in vitro. Previously, low nanomolar antiviral efficacy was revealed when tested against multiple viral variants in several cell types. SARS-CoV-2 RNA isolated from live virus infected cells and the virions released from these cells was analyzed by mass spectrometry (MS) for S incorporation. Dose-dependent incorporation occurred up to 1.8 S per 1,000 nucleotides (49 S per genome) throughout the viral genomes i...
Source: Antiviral Research - September 10, 2023 Category: Virology Authors: Ryan P Bennett Yasemin Yolu ç Jason D Salter Alexander Ripp Henning J Jessen Stefanie M Kaiser Harold C Smith Source Type: research

Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase
Antiviral Res. 2023 Sep 8:105716. doi: 10.1016/j.antiviral.2023.105716. Online ahead of print.ABSTRACTSangivamycin (S) is an adenosine (A) nucleoside analog with low nanomolar antiviral activity against SARS-CoV-2 in vitro. Previously, low nanomolar antiviral efficacy was revealed when tested against multiple viral variants in several cell types. SARS-CoV-2 RNA isolated from live virus infected cells and the virions released from these cells was analyzed by mass spectrometry (MS) for S incorporation. Dose-dependent incorporation occurred up to 1.8 S per 1,000 nucleotides (49 S per genome) throughout the viral genomes isola...
Source: Antiviral Research - September 10, 2023 Category: Virology Authors: Ryan P Bennett Yasemin Yolu ç Jason D Salter Alexander Ripp Henning J Jessen Stefanie M Kaiser Harold C Smith Source Type: research

Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase
Antiviral Res. 2023 Sep 8:105716. doi: 10.1016/j.antiviral.2023.105716. Online ahead of print.ABSTRACTSangivamycin (S) is an adenosine (A) nucleoside analog with low nanomolar antiviral activity against SARS-CoV-2 in vitro. Previously, low nanomolar antiviral efficacy was revealed when tested against multiple viral variants in several cell types. SARS-CoV-2 RNA isolated from live virus infected cells and the virions released from these cells was analyzed by mass spectrometry (MS) for S incorporation. Dose-dependent incorporation occurred up to 1.8 S per 1,000 nucleotides (49 S per genome) throughout the viral genomes isola...
Source: Antiviral Research - September 10, 2023 Category: Virology Authors: Ryan P Bennett Yasemin Yolu ç Jason D Salter Alexander Ripp Henning J Jessen Stefanie M Kaiser Harold C Smith Source Type: research

Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase
Antiviral Res. 2023 Sep 8:105716. doi: 10.1016/j.antiviral.2023.105716. Online ahead of print.ABSTRACTSangivamycin (S) is an adenosine (A) nucleoside analog with low nanomolar antiviral activity against SARS-CoV-2 in vitro. Previously, low nanomolar antiviral efficacy was revealed when tested against multiple viral variants in several cell types. SARS-CoV-2 RNA isolated from live virus infected cells and the virions released from these cells was analyzed by mass spectrometry (MS) for S incorporation. Dose-dependent incorporation occurred up to 1.8 S per 1,000 nucleotides (49 S per genome) throughout the viral genomes isola...
Source: Antiviral Research - September 10, 2023 Category: Virology Authors: Ryan P Bennett Yasemin Yolu ç Jason D Salter Alexander Ripp Henning J Jessen Stefanie M Kaiser Harold C Smith Source Type: research

Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase
Antiviral Res. 2023 Sep 8:105716. doi: 10.1016/j.antiviral.2023.105716. Online ahead of print.ABSTRACTSangivamycin (S) is an adenosine (A) nucleoside analog with low nanomolar antiviral activity against SARS-CoV-2 in vitro. Previously, low nanomolar antiviral efficacy was revealed when tested against multiple viral variants in several cell types. SARS-CoV-2 RNA isolated from live virus infected cells and the virions released from these cells was analyzed by mass spectrometry (MS) for S incorporation. Dose-dependent incorporation occurred up to 1.8 S per 1,000 nucleotides (49 S per genome) throughout the viral genomes isola...
Source: Antiviral Research - September 10, 2023 Category: Virology Authors: Ryan P Bennett Yasemin Yolu ç Jason D Salter Alexander Ripp Henning J Jessen Stefanie M Kaiser Harold C Smith Source Type: research

Sangivamycin is preferentially incorporated into viral RNA by the SARS-CoV-2 polymerase
Antiviral Res. 2023 Sep 8:105716. doi: 10.1016/j.antiviral.2023.105716. Online ahead of print.ABSTRACTSangivamycin (S) is an adenosine (A) nucleoside analog with low nanomolar antiviral activity against SARS-CoV-2 in vitro. Previously, low nanomolar antiviral efficacy was revealed when tested against multiple viral variants in several cell types. SARS-CoV-2 RNA isolated from live virus infected cells and the virions released from these cells was analyzed by mass spectrometry (MS) for S incorporation. Dose-dependent incorporation occurred up to 1.8 S per 1,000 nucleotides (49 S per genome) throughout the viral genomes isola...
Source: Antiviral Research - September 10, 2023 Category: Virology Authors: Ryan P Bennett Yasemin Yolu ç Jason D Salter Alexander Ripp Henning J Jessen Stefanie M Kaiser Harold C Smith Source Type: research

Inhibitors of mpox VP39 2'-O methyltransferase efficiently inhibit the monkeypox virus
In this study, we employed plaque assays and cytopathic effect-based assays to evaluate the effectiveness of these compounds. All tested compounds demonstrated antiviral activity against MpxV, with EC50 values ranging from 0.06 to 2.7 μM. Nevertheless, some of these compounds also exhibited cytotoxicity in HeLa cells, while others showed no toxicity. Notably, the non-toxic compounds featured a large aromatic substituent at the 7-deaza position, whereas the toxic compounds had a small substituent at the same position. These findings suggest that VP39 represents a bona fide target for the development of antiviral drugs agai...
Source: Antiviral Research - September 9, 2023 Category: Virology Authors: Michala Zgarbov á Tomas Otava Jan Silhan Radim Nencka Jan Weber Evzen Boura Source Type: research