Analytical and Bioanalytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Use of 3D printing to integrate microchip electrophoresis with amperometric detection
Anal Bioanal Chem. 2024 Apr 6. doi: 10.1007/s00216-024-05260-6. Online ahead of print.ABSTRACTThis paper describes the use of PolyJet 3D printing to fabricate microchip electrophoresis devices with integrated microwire electrodes for amperometric detection. The fabrication process involves 3D printing of two separate pieces, a channel layer and an electrode layer. The channel layer is created by 3D printing on a pre-fabricated mold with a T-intersection. For the electrode layer, a stencil design is printed directly on the printing tray and covered with a piece of transparent glass. Microwire electrodes are adhered over the...
Source: Analytical and Bioanalytical Chemistry - April 6, 2024 Category: Chemistry Authors: Major A Selemani R Scott Martin Source Type: research

Carbon dots with enhanced red emission for ratiometric sensing and encryption applications
Anal Bioanal Chem. 2024 Apr 6. doi: 10.1007/s00216-024-05252-6. Online ahead of print.ABSTRACTThe excitation-dependent emission properties of carbon dots (Cdots) are extensively reported, but their red emission is often weak, limiting their wider application. Here we introduce ethidium bromide, as a functional precursor with red emission, to enhance the red emission for Cdots, with comparable intensity at a broad wavelength range to multi-emission Cdots (M-Cdots). We found that Cdots prepared with ethidium bromide/ethylenediamine exhibited strong blue and red emission at 440 and 615 nm, with optimal excitation at 360 and 4...
Source: Analytical and Bioanalytical Chemistry - April 6, 2024 Category: Chemistry Authors: Sheng-Nan Zhang Lin-Lin Wang Ting-Ting Xiao Min Zhang Xue-Bo Yin Source Type: research

Multi-element analysis of unfiltered samples in river water monitoring-digestion and single-run analyses of 67 elements
In this study, we provide several digestion protocols for whole water samples from rivers and the subsequent multi-element analysis of 67 major, minor, and trace elements: Li, Be, B, Na, Mg, Al, Si, P, S, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, As, Se, Br, Rb, Sr, Y, Zr, Nb, Mo, Ru, Ag, Cd, In, Sn, Sb, Te, Cs, Ba, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Hf, Ta, W, Ir, Pt, Au, Hg, Tl, Pb, Bi, Th, U. In the absence of whole water reference materials for inorganic analytes, we introduce simulated whole water samples by suspending sediment reference materials as quality control measures. The appli...
Source: Analytical and Bioanalytical Chemistry - April 5, 2024 Category: Chemistry Authors: Nadine Belkouteb Henning Schroeder Jan G Wiederhold Thomas A Ternes Lars Duester Source Type: research

Assessment of monoclonal antibody glycosylation: a comparative study using HRMS, NMR, and HILIC-FLD
In this study, two products were compared using a panel of analytical methods. Two high-resolution mass spectrometry (HRMS) workflows were used to analyze N-glycans, while nuclear magnetic resonance (NMR) was used to generate monosaccharide fingerprints. These state-of-the-art techniques were compared to conventional analysis using hydrophilic interaction chromatography (HILIC) coupled with fluorescence detection (FLD). The advantages and disadvantages of each method are discussed along with a comparison of the identified glycan distributions. The results demonstrated agreement across all methods for major glycoforms, demo...
Source: Analytical and Bioanalytical Chemistry - April 5, 2024 Category: Chemistry Authors: Joshua Shipman Michael Karfunkle Hongbin Zhu You Zhuo Kang Chen Milani Patabandige Di Wu Mercy Oyugi Richard Kerr Kui Yang Sarah Rogstad Source Type: research

Multi-element analysis of unfiltered samples in river water monitoring-digestion and single-run analyses of 67 elements
In this study, we provide several digestion protocols for whole water samples from rivers and the subsequent multi-element analysis of 67 major, minor, and trace elements: Li, Be, B, Na, Mg, Al, Si, P, S, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Ge, As, Se, Br, Rb, Sr, Y, Zr, Nb, Mo, Ru, Ag, Cd, In, Sn, Sb, Te, Cs, Ba, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Hf, Ta, W, Ir, Pt, Au, Hg, Tl, Pb, Bi, Th, U. In the absence of whole water reference materials for inorganic analytes, we introduce simulated whole water samples by suspending sediment reference materials as quality control measures. The appli...
Source: Analytical and Bioanalytical Chemistry - April 5, 2024 Category: Chemistry Authors: Nadine Belkouteb Henning Schroeder Jan G Wiederhold Thomas A Ternes Lars Duester Source Type: research

Assessment of monoclonal antibody glycosylation: a comparative study using HRMS, NMR, and HILIC-FLD
In this study, two products were compared using a panel of analytical methods. Two high-resolution mass spectrometry (HRMS) workflows were used to analyze N-glycans, while nuclear magnetic resonance (NMR) was used to generate monosaccharide fingerprints. These state-of-the-art techniques were compared to conventional analysis using hydrophilic interaction chromatography (HILIC) coupled with fluorescence detection (FLD). The advantages and disadvantages of each method are discussed along with a comparison of the identified glycan distributions. The results demonstrated agreement across all methods for major glycoforms, demo...
Source: Analytical and Bioanalytical Chemistry - April 5, 2024 Category: Chemistry Authors: Joshua Shipman Michael Karfunkle Hongbin Zhu You Zhuo Kang Chen Milani Patabandige Di Wu Mercy Oyugi Richard Kerr Kui Yang Sarah Rogstad Source Type: research

Batch-to-batch reproducibility for the primary pH method for the example of NMIJ carbonate buffer solutions
Anal Bioanal Chem. 2024 Apr 4. doi: 10.1007/s00216-024-05276-y. Online ahead of print.ABSTRACTAt present, the National Metrology Institute of Japan provides six national primary pH buffers under the Japan Calibration Service System. Each batch of these buffers is certified by the primary pH method using a Harned cell. On the basis of these primary buffers, the designated laboratories supply the secondary and working pH standards using a high-precision pH meter. This paper provides an estimate of the batch-to-batch reproducibility of the primary pH standard production based on the history of the certification of primary car...
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Toshiaki Asakai Igor Maksimov Sachiko Onuma Source Type: research

Development of an innovative analytical method for forensic detection of cocaine, antidepressants, and metabolites in postmortem blood using magnetic nanoparticles
This study focused on developing an innovative analytical method for the determination of cocaine, antidepressants, and their related metabolites in postmortem blood samples, using unmodified commercial Fe3O4 nanoparticles as a sorbent for dispersive magnetic solid-phase extraction (m-d-SPE), coupled with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis. An aliquot of 100 µL of whole blood and 5 µL of the internal standard pool were added to 30 mg of nanoparticles. The nanoparticles were separated from the sample using a neodymium magnet inserted into a 3D-printed microtube rack. The liquid...
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Patricia de Souza Schwarz Bruno Pereira Dos Santos Let ícia Birk Sarah Eller Tiago Franco de Oliveira Source Type: research

Online monitoring of protein refolding in inclusion body processing using intrinsic fluorescence
Anal Bioanal Chem. 2024 Apr 4. doi: 10.1007/s00216-024-05249-1. Online ahead of print.ABSTRACTInclusion bodies (IBs) are protein aggregates formed as a result of overexpression of recombinant protein in E. coli. The formation of IBs is a valuable strategy of recombinant protein production despite the need for additional processing steps, i.e., isolation, solubilization and refolding. Industrial process development of protein refolding is a labor-intensive task based largely on empirical approaches rather than knowledge-driven strategies. A prerequisite for knowledge-driven process development is a reliable monitoring strat...
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Chika Linda Igwe Don Fabian M üller Florian Gisperg Jan Niklas Pauk Matthias Kierein Mohamed Elshazly Robert Klausser Julian Kopp Oliver Spadiut Eva P řáda Brichtová Source Type: research

Screening anabolic androgenic steroids in human urine: an application of the state-of-the-art gas chromatography-Orbitrap high-resolution mass spectrometry
This study provides a rationale for GC-Orbitrap-HRMS in drug metabolism and non-targeted screening.PMID:38573345 | DOI:10.1007/s00216-024-05272-2 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Ziyi Ji Lei Liao Yuqi Ge Mengpan Liu Xian Fang Haoyi Sun Siying Zheng Xiaojun Deng Source Type: research

Batch-to-batch reproducibility for the primary pH method for the example of NMIJ carbonate buffer solutions
Anal Bioanal Chem. 2024 Apr 4. doi: 10.1007/s00216-024-05276-y. Online ahead of print.ABSTRACTAt present, the National Metrology Institute of Japan provides six national primary pH buffers under the Japan Calibration Service System. Each batch of these buffers is certified by the primary pH method using a Harned cell. On the basis of these primary buffers, the designated laboratories supply the secondary and working pH standards using a high-precision pH meter. This paper provides an estimate of the batch-to-batch reproducibility of the primary pH standard production based on the history of the certification of primary car...
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Toshiaki Asakai Igor Maksimov Sachiko Onuma Source Type: research

Development of an innovative analytical method for forensic detection of cocaine, antidepressants, and metabolites in postmortem blood using magnetic nanoparticles
This study focused on developing an innovative analytical method for the determination of cocaine, antidepressants, and their related metabolites in postmortem blood samples, using unmodified commercial Fe3O4 nanoparticles as a sorbent for dispersive magnetic solid-phase extraction (m-d-SPE), coupled with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis. An aliquot of 100 µL of whole blood and 5 µL of the internal standard pool were added to 30 mg of nanoparticles. The nanoparticles were separated from the sample using a neodymium magnet inserted into a 3D-printed microtube rack. The liquid...
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Patricia de Souza Schwarz Bruno Pereira Dos Santos Let ícia Birk Sarah Eller Tiago Franco de Oliveira Source Type: research

Online monitoring of protein refolding in inclusion body processing using intrinsic fluorescence
Anal Bioanal Chem. 2024 Apr 4. doi: 10.1007/s00216-024-05249-1. Online ahead of print.ABSTRACTInclusion bodies (IBs) are protein aggregates formed as a result of overexpression of recombinant protein in E. coli. The formation of IBs is a valuable strategy of recombinant protein production despite the need for additional processing steps, i.e., isolation, solubilization and refolding. Industrial process development of protein refolding is a labor-intensive task based largely on empirical approaches rather than knowledge-driven strategies. A prerequisite for knowledge-driven process development is a reliable monitoring strat...
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Chika Linda Igwe Don Fabian M üller Florian Gisperg Jan Niklas Pauk Matthias Kierein Mohamed Elshazly Robert Klausser Julian Kopp Oliver Spadiut Eva P řáda Brichtová Source Type: research

Screening anabolic androgenic steroids in human urine: an application of the state-of-the-art gas chromatography-Orbitrap high-resolution mass spectrometry
This study provides a rationale for GC-Orbitrap-HRMS in drug metabolism and non-targeted screening.PMID:38573345 | DOI:10.1007/s00216-024-05272-2 (Source: Analytical and Bioanalytical Chemistry)
Source: Analytical and Bioanalytical Chemistry - April 4, 2024 Category: Chemistry Authors: Ziyi Ji Lei Liao Yuqi Ge Mengpan Liu Xian Fang Haoyi Sun Siying Zheng Xiaojun Deng Source Type: research

Development of a corn flour certified reference material for the accurate determination of zearalenone
Anal Bioanal Chem. 2024 Apr 3. doi: 10.1007/s00216-024-05265-1. Online ahead of print.ABSTRACTA certified reference material (CRM, KRISS 108-01-002) for zearalenone in corn flour was developed to assure reliable and accurate measurements in testing laboratories. Commercially available corn flour underwent freeze-drying, pulverization, sieving, and homogenization. The final product was packed in amber bottles, approximately 14 g per unit, and preserved at -70 °C. 13C18-Zearalenone was used as an internal standard (IS) for the certification of zearalenone by isotope-dilution liquid chromatography-tandem mass spectrometry (I...
Source: Analytical and Bioanalytical Chemistry - April 3, 2024 Category: Chemistry Authors: Yared Getachew Lijalem Mohamed A Gab-Allah Hyeonwoo Yu Kihwan Choi Byungjoo Kim Source Type: research